pycom04g19180

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012633
Physical Location & Seq
Forward (+)
20943498 .. 20947054
3557 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2076 bp
ATGTCGATCACCGTCGACAAAATGTCCGGCGACCTCCACATGACGGCGGAGCTCCGCAAATCGTTCAACCTCCTCAAGACGCACGCCGCCAAAGTTGCCAACTTTACCCTCCAATGGCAGGACGTCGAGGATCACTTCCACTCCATCGACGATTCCATCCAGTCCGAGCTCGACCAACTGCAAAAACAACAATCGGAAGAGACCCATCTCACTCCGGATCAGCAATCGGAACCCAAAGAACCCGAATTGAATTTCAAACCCAATTCTGAAGGACGCCAATTGAGTTCCGGTGGCGCCCAATTGAGTTCGAAAGAATCCCAACTCACCGCCACCCTCCAATCCTTGAAAACCCAATTCGATTCCGTCGAAACCCAAGTCAAAACCGATGAGCGTTTGCCGGAAATGTCAACCTGCAATGGCGTCCCGATAAATGACGGCAAGGCGTTGCTGTTGTATGTGAATGAGCATTTGAAAGAGAGGGATTCGGTGCGTGACCGTGTTTCGGATGCGCTTAAGGTTTCTGTGGATGCTGGGAAGCTAGTGCTTGAGGCATTGAGATGGTTTTATCCATCGGAGTTGAATAAGGGAGGTAAGGAGCTTGATGTCACTGTTACTAGGAAAAGTTGTGTGATTTTGTTACAAGAGCTGGTGAATGTGAAACCAGTGATTAAACCCGATGTCAGCAGAGAGGCACTGAAGCTGGCATTGGCTTGGAAGGTGAAGGTGATGATGACTCAAATGTCGAACTCTTTGGAGGTTTGGGCTTTTTTGCAGCTTTTGGATACTTTTCAGTTGGGTGGTGAGTTTGACAGTGATGAGATTTTGATGTTTGTTAGTTGCGTAGCGGAGCGGAATTATGCACCAGAGTTGTTTAGGTCTCTTGGTTTCACCGATAAAGCCTCTGATCTCATCCAAAAGCTTGTGTCAGAGAATAAGCGCCTTGATGCTGTTAGATTCAGTCATGCATTTGGACAGTTTGACAAGTTCCCCCCTGTACCCTTGCTTAAAGCACATTTAAAGTTCGTCACCAAAGATGCAAGGACAAGTTCCAAGAGGGGCCAAGAGCGTCTTAAAGCACAGGATGAGGCTAGAGACAAAGAAATAGCTGCTCTAAGAGGTATAATACGATGCATTGAGGCCTACAAGCTGCTTGTTGAACCACAATATTCTCCTGAGACCCTCAGAAAACGCATCAAGCAGCTGAGACGGCAGAGAAAAGAGAGGAAAGCAACTCTGACATCTCCTGGCCCTGAGGCTCAACCGCAATCCCTGAGTGGAAAGAAACGTACTGCCCCGCCTCAATCAAATAAGCAGAACAGAAATAAGCATCCTCGAACAGCTCCAGCTTTAGCTGCTCAAACTCTTTCTTTCAGAGGACCAGCCACAGTACGCTCTGTACAACCATCTTACATTCGGCAAGTAAGCTTGTATGACAGTCGAAGTGCAGAATACTTGTTATCATCCGCAGGAATGTCGGGACAAGCAACGACTCCTAATACTTCTTATGGCACCATTTCCACCTTGAACCCATTGCGTGCATCTCATCATCAGCAAGGAGGCTCATTCATGGGTCAAGGTGCAGAATACTCTGCTCGATACTATGATTCAGCGCACTACGCTCCTGACATTAATAATGACTTATCTGCTCGATCATATGACTTGGTGGGCTCCTCTCCTGGTACCCGTACCCAGCACTTGAACTTGACAGCTCGAGACTATGGATTGACGGCTCCGTCTCATGTACATACGAACTCAGCAATTGCATCTTATGGACTCGGGGGCAGTAGTCATGTAACTTCAGATATAAGCCCATTAGTCGCACGATACGGGGCAAGTATAGCTGCAAATGGAAGGACTGGACGGTTTGGATCGGCTGGAAGCCGTACACCTGTGCGCAATGCAAGCCCGAATAGTTCCATCCCTTATGCGTATAGGGACCATGCAAGCCCGAATAGTTCCATCCCTTATGCGTATAGGGATCCGGTGAGGGCACCTAACCACAACGACCGGTCAGTGCCTTCCAGCAGTGGCCATGGCGCGCCGTCTCAGCACCCCCCGGCCATTTATCACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

692

Amino Acids

76.45

Weight (kDa)

9.29

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 145 - 411 3.6e-74 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 739, 1732
AatII GACGTC 1 cut(s) 126
Acc16I TGCGCA 1 cut(s) 1895
Acc36I ACCTGC 1 cut(s) 419
Acc65I GGTACC 1 cut(s) 1679
AccB1I GGYRCC 4 cut(s) 293, 1508, 1679, 1990
AccBSI CCGCTC 1 cut(s) 850
AccI GTMKAC 1 cut(s) 15
AccII CGCG 1 cut(s) 2039
AccIII TCCGGA 1 cut(s) 214
AciI CCGC 9 cut(s) 47, 55, 87, 327, 845, 850, 1262, 1295, 1464
AclWI GGATC 5 cut(s) 138, 225, 1876, 1973, 1986
AcoI YGGCCR 2 cut(s) 2029, 2058
AcsI RAATTY 1 cut(s) 250
AcuI CTGAAG 3 cut(s) 288, 716, 1782
AcyI GRCGYC 4 cut(s) 123, 274, 294, 420
AfaI GTAC 8 cut(s) 996, 1288, 1389, 1398, 1681, 1687, 1743, 1885
AfiI CCNNNNNNNGG 4 cut(s) 43, 114, 118, 502
AflII CTTAAG 1 cut(s) 512
AgeI ACCGGT 1 cut(s) 2007
AgsI TTSAA 9 cut(s) 67, 250, 256, 346, 472, 580, 1157, 1525, 1699
AhdI GACNNNNNGTC 1 cut(s) 22
AjnI CCWGG 2 cut(s) 1243, 1675
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
Alw21I GWGCWC 2 cut(s) 54, 171
Alw26I GTCTC 8 cut(s) 194, 882, 1086, 1169, 1198, 1707, 1740, 2049
AlwI GGATC 5 cut(s) 138, 225, 1876, 1973, 1986
Ama87I CYCGRG 2 cut(s) 1710, 1775
Aor13HI TCCGGA 1 cut(s) 214
AoxI GGCC 5 cut(s) 1057, 1137, 1246, 2029, 2058
ApeKI GCWGC 6 cut(s) 772, 1106, 1147, 1198, 1352, 1841
ApoI RAATTY 1 cut(s) 250
ArsI GACNNNNNNTTYG 2 cut(s) 360, 392
AscI GGCGCGCC 1 cut(s) 2037
AseI ATTAAT 1 cut(s) 1629
AsiGI ACCGGT 1 cut(s) 2007
Asp718I GGTACC 1 cut(s) 1679
AspLEI GCGC 7 cut(s) 296, 511, 939, 1612, 1896, 2039, 2041
AspS9I GGNCC 4 cut(s) 1057, 1247, 1376, 1936
AsuC2I CCSGG 1 cut(s) 2057
AsuHPI GGTGA 9 cut(s) 316, 661, 730, 736, 812, 880, 1018, 1996, 2060
AsuII TTCGAA 1 cut(s) 308
AvaI CYCGRG 2 cut(s) 1710, 1775
AvaII GGWCC 2 cut(s) 1376, 1936
AxyI CCTNAGG 1 cut(s) 1251
BaeGI GKGCMC 1 cut(s) 1993
BalI TGGCCA 1 cut(s) 2031
BamHI GGATCC 1 cut(s) 1978
BanI GGYRCC 4 cut(s) 293, 1508, 1679, 1990
BanII GRGCYC 3 cut(s) 54, 171, 1670
Bbv12I GWGCWC 2 cut(s) 54, 171
BbvI GCAGC 6 cut(s) 784, 1093, 1134, 1210, 1339, 1828
BccI CCATC 8 cut(s) 152, 164, 213, 552, 577, 1411, 1925, 1967
BceAI ACGGC 6 cut(s) 60, 451, 1223, 1743, 1866, 2026
BcgI CGANNNNNNTGC 2 cut(s) 1455, 1489
BciT130I CCWGG 2 cut(s) 1245, 1677
BciVI GTATCC 1 cut(s) 775
BcnI CCSGG 1 cut(s) 2057
BcoDI GTCTC 8 cut(s) 194, 882, 1086, 1169, 1198, 1707, 1740, 2049
BfaI CTAG 3 cut(s) 539, 615, 1089
BfoI RGCGCY 2 cut(s) 297, 940
BfrI CTTAAG 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 419
BfuI GTATCC 1 cut(s) 775
BisI GCNGC 7 cut(s) 87, 773, 1107, 1148, 1199, 1353, 1842
BlsI GCNGC 7 cut(s) 88, 774, 1108, 1149, 1200, 1354, 1843
Bme1390I CCNGG 3 cut(s) 1245, 1677, 2057
Bme18I GGWCC 2 cut(s) 1376, 1936
BmeRI GACNNNNNGTC 1 cut(s) 22
BmeT110I CYCGRG 2 cut(s) 1710, 1775
BmgT120I GGNCC 4 cut(s) 1057, 1247, 1376, 1936
BmrFI CCNGG 3 cut(s) 1245, 1677, 2057
BmsI GCATC 9 cut(s) 496, 517, 934, 1024, 1118, 1200, 1336, 1547, 1772
BpmI CTGGAG 1 cut(s) 1326
Bpu14I TTCGAA 1 cut(s) 308
BpuEI CTTGAG 2 cut(s) 59, 566
BpuMI CCSGG 1 cut(s) 2057
BsaHI GRCGYC 4 cut(s) 123, 274, 294, 420
BsaI GGTCTC 3 cut(s) 194, 882, 1169
BsaJI CCNNGG 2 cut(s) 2032, 2055
BsaWI WCCGGW 4 cut(s) 214, 287, 1981, 2007
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 114, 118, 502
Bse118I RCCGGY 1 cut(s) 2007
Bse1I ACTGG 3 cut(s) 160, 662, 1861
Bse21I CCTNAGG 1 cut(s) 1251
Bse3DI GCAATG 3 cut(s) 421, 1529, 1903
BseAI TCCGGA 1 cut(s) 214
BseBI CCWGG 2 cut(s) 1245, 1677
BseDI CCNNGG 2 cut(s) 2032, 2055
BseGI GGATG 9 cut(s) 156, 511, 532, 909, 1087, 1327, 1460, 1917, 1959
BseLI CCNNNNNNNGG 4 cut(s) 43, 114, 118, 502
BseMI GCAATG 3 cut(s) 421, 1529, 1903
BseMII CTCAG 7 cut(s) 1164, 1193, 1195, 1242, 1262, 1767, 2060
BseNI ACTGG 3 cut(s) 160, 662, 1861
BsePI GCGCGC 1 cut(s) 2037
BseRI GAGGAG 2 cut(s) 62, 1660
BseSI GKGCMC 1 cut(s) 1993
BseXI GCAGC 6 cut(s) 784, 1093, 1134, 1210, 1339, 1828
BseYI CCCAGC 2 cut(s) 530, 1689
BsgI GTGCAG 2 cut(s) 1464, 1599
Bsh1236I CGCG 1 cut(s) 2039
Bsh1285I CGRYCG 1 cut(s) 2008
BshFI GGCC 5 cut(s) 1059, 1139, 1248, 2031, 2060
BshNI GGYRCC 4 cut(s) 293, 1508, 1679, 1990
BshTI ACCGGT 1 cut(s) 2007
BsiEI CGRYCG 1 cut(s) 2008
BsiHKAI GWGCWC 2 cut(s) 54, 171
BsiHKCI CYCGRG 2 cut(s) 1710, 1775
BsiSI CCGG 7 cut(s) 27, 215, 288, 398, 1982, 2008, 2057
BslFI GGGAC 3 cut(s) 407, 1491, 1949
BslI CCNNNNNNNGG 4 cut(s) 43, 114, 118, 502
BsmAI GTCTC 8 cut(s) 194, 882, 1086, 1169, 1198, 1707, 1740, 2049
BsmBI CGTCTC 3 cut(s) 1198, 1740, 2049
BsmFI GGGAC 3 cut(s) 407, 1491, 1949
BsnI GGCC 5 cut(s) 1059, 1139, 1248, 2031, 2060
Bso31I GGTCTC 3 cut(s) 194, 882, 1169
BsoBI CYCGRG 2 cut(s) 1710, 1775
Bsp119I TTCGAA 1 cut(s) 308
Bsp1286I GDGCHC 4 cut(s) 54, 171, 1670, 1993
Bsp13I TCCGGA 1 cut(s) 214
Bsp1407I TGTACA 2 cut(s) 1396, 1741
Bsp143I GATC 7 cut(s) 6, 130, 217, 904, 1649, 1868, 1978
Bsp19I CCATGG 1 cut(s) 2032
BspACI CCGC 9 cut(s) 47, 55, 87, 327, 845, 850, 1262, 1295, 1464
BspANI GGCC 5 cut(s) 1059, 1139, 1248, 2031, 2060
BspCNI CTCAG 7 cut(s) 1165, 1194, 1194, 1243, 1263, 1766, 2059
BspEI TCCGGA 1 cut(s) 214
BspFNI CGCG 1 cut(s) 2039
BspMI ACCTGC 1 cut(s) 419
BspPI GGATC 5 cut(s) 138, 225, 1876, 1973, 1986
BspT104I TTCGAA 1 cut(s) 308
BspT107I GGYRCC 4 cut(s) 293, 1508, 1679, 1990
BspTI CTTAAG 1 cut(s) 512
BspTNI GGTCTC 3 cut(s) 194, 882, 1169
BsrBI CCGCTC 1 cut(s) 850
BsrDI GCAATG 3 cut(s) 421, 1529, 1903
BsrFI RCCGGY 1 cut(s) 2007
BsrGI TGTACA 2 cut(s) 1396, 1741
BsrI ACTGG 3 cut(s) 160, 662, 1861
BssAI RCCGGY 1 cut(s) 2007
BssECI CCNNGG 2 cut(s) 2032, 2055
BssHII GCGCGC 1 cut(s) 2037
BssMI GATC 7 cut(s) 6, 130, 217, 904, 1649, 1868, 1978
BssNI GRCGYC 4 cut(s) 123, 274, 294, 420
BssT1I CCWWGG 1 cut(s) 2032
Bst2UI CCWGG 2 cut(s) 1245, 1677
Bst4CI ACNGT 8 cut(s) 13, 497, 610, 812, 975, 1387, 1436, 1863
Bst6I CTCTTC 1 cut(s) 192
BstACI GRCGYC 4 cut(s) 123, 274, 294, 420
BstAFI CTTAAG 1 cut(s) 512
BstAUI TGTACA 2 cut(s) 1396, 1741
BstBI TTCGAA 1 cut(s) 308
BstC8I GCNNGC 6 cut(s) 84, 702, 1536, 1903, 1945, 2039
BstDEI CTNAG 8 cut(s) 1112, 1173, 1181, 1202, 1251, 1271, 1753, 2046
BstDSI CCRYGG 1 cut(s) 2032
BstF5I GGATG 9 cut(s) 156, 511, 532, 909, 1087, 1327, 1460, 1917, 1959
BstFNI CGCG 1 cut(s) 2039
BstH2I RGCGCY 2 cut(s) 297, 940
BstHHI GCGC 7 cut(s) 296, 511, 939, 1612, 1896, 2039, 2041
BstKTI GATC 7 cut(s) 9, 133, 220, 907, 1652, 1871, 1981
BstMAI GTCTC 8 cut(s) 194, 882, 1086, 1169, 1198, 1707, 1740, 2049
BstMBI GATC 7 cut(s) 6, 130, 217, 904, 1649, 1868, 1978
BstMCI CGRYCG 1 cut(s) 2008
BstMWI GCNNNNNNNGC 7 cut(s) 95, 1207, 1352, 1616, 1838, 1902, 2047
BstNI CCWGG 2 cut(s) 1245, 1677
BstSCI CCNGG 3 cut(s) 1243, 1675, 2055
BstSLI GKGCMC 1 cut(s) 1993
BstUI CGCG 1 cut(s) 2039
BstV1I GCAGC 6 cut(s) 784, 1093, 1134, 1210, 1339, 1828
BstX2I RGATCY 1 cut(s) 1978
BstYI RGATCY 1 cut(s) 1978
Bsu36I CCTNAGG 1 cut(s) 1251
BsuI GTATCC 1 cut(s) 775
BsuRI GGCC 5 cut(s) 1059, 1139, 1248, 2031, 2060
BtgI CCRYGG 1 cut(s) 2032
BtsCI GGATG 9 cut(s) 156, 511, 532, 909, 1087, 1327, 1460, 1917, 1959
BtsI GCAGTG 1 cut(s) 2032
BtsIMutI CAGTG 6 cut(s) 606, 669, 692, 817, 2019, 2032
BveI ACCTGC 1 cut(s) 419
Cac8I GCNNGC 6 cut(s) 84, 702, 1536, 1903, 1945, 2039
CfoI GCGC 7 cut(s) 296, 511, 939, 1612, 1896, 2039, 2041
Cfr10I RCCGGY 1 cut(s) 2007
Cfr13I GGNCC 4 cut(s) 1057, 1247, 1376, 1936
CseI GACGC 4 cut(s) 88, 282, 409, 1055
Csp6I GTAC 8 cut(s) 995, 1287, 1388, 1397, 1680, 1686, 1742, 1884
CspAI ACCGGT 1 cut(s) 2007
CviAII CATG 7 cut(s) 40, 962, 1567, 1739, 1790, 1940, 2033
CviQI GTAC 8 cut(s) 995, 1287, 1388, 1397, 1680, 1686, 1742, 1884
DdeI CTNAG 8 cut(s) 1112, 1173, 1181, 1202, 1251, 1271, 1753, 2046
DinI GGCGCC 1 cut(s) 295
DpnI GATC 7 cut(s) 8, 132, 219, 906, 1651, 1870, 1980
DpnII GATC 7 cut(s) 6, 130, 217, 904, 1649, 1868, 1978
DraI TTTAAA 1 cut(s) 1017
DrdI GACNNNNNNGTC 2 cut(s) 739, 1732
DriI GACNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 2 cut(s) 739, 1732
EaeI YGGCCR 2 cut(s) 2029, 2058
Eam1104I CTCTTC 1 cut(s) 192
Eam1105I GACNNNNNGTC 1 cut(s) 22
EarI CTCTTC 1 cut(s) 192
EciI GGCGGA 1 cut(s) 62
Ecl136II GAGCTC 2 cut(s) 52, 169
Eco130I CCWWGG 1 cut(s) 2032
Eco147I AGGCCT 1 cut(s) 1139
Eco24I GRGCYC 3 cut(s) 54, 171, 1670
Eco31I GGTCTC 3 cut(s) 194, 882, 1169
Eco47I GGWCC 2 cut(s) 1376, 1936
Eco53kI GAGCTC 2 cut(s) 52, 169
Eco57I CTGAAG 3 cut(s) 288, 716, 1782
Eco81I CCTNAGG 1 cut(s) 1251
Eco88I CYCGRG 2 cut(s) 1710, 1775
EcoICRI GAGCTC 2 cut(s) 52, 169
EcoRII CCWGG 2 cut(s) 1243, 1675
EcoT14I CCWWGG 1 cut(s) 2032
EcoT22I ATGCAT 2 cut(s) 967, 1133
EcoT38I GRGCYC 3 cut(s) 54, 171, 1670
EgeI GGCGCC 1 cut(s) 295
EheI GGCGCC 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 2032
Esp3I CGTCTC 3 cut(s) 1198, 1740, 2049
FaeI CATG 7 cut(s) 43, 965, 1570, 1742, 1793, 1943, 2036
FalI AAGNNNNNCTT 2 cut(s) 1053, 1085
FaqI GGGAC 3 cut(s) 407, 1491, 1949
FatI CATG 7 cut(s) 39, 961, 1566, 1738, 1789, 1939, 2032
FauI CCCGC 1 cut(s) 1302
FauNDI CATATG 1 cut(s) 1654
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 7 cut(s) 87, 773, 1107, 1148, 1199, 1353, 1842
FokI GGATG 9 cut(s) 143, 518, 539, 896, 1094, 1314, 1447, 1904, 1946
FriOI GRGCYC 3 cut(s) 54, 171, 1670
Fsp4HI GCNGC 7 cut(s) 87, 773, 1107, 1148, 1199, 1353, 1842
FspBI CTAG 3 cut(s) 539, 615, 1089
FspI TGCGCA 1 cut(s) 1895
GlaI GCGC 7 cut(s) 295, 510, 938, 1611, 1895, 2038, 2040
GluI GCNGC 7 cut(s) 87, 773, 1107, 1148, 1199, 1353, 1842
GsaI CCCAGC 2 cut(s) 534, 1693
GsuI CTGGAG 1 cut(s) 1326
HaeII RGCGCY 2 cut(s) 297, 940
HaeIII GGCC 5 cut(s) 1059, 1139, 1248, 2031, 2060
HapII CCGG 7 cut(s) 27, 215, 288, 398, 1982, 2008, 2057
HgaI GACGC 4 cut(s) 88, 282, 409, 1055
HhaI GCGC 7 cut(s) 296, 511, 939, 1612, 1896, 2039, 2041
Hin1I GRCGYC 4 cut(s) 123, 274, 294, 420
Hin1II CATG 7 cut(s) 43, 965, 1570, 1742, 1793, 1943, 2036
Hin6I GCGC 7 cut(s) 294, 509, 937, 1610, 1894, 2037, 2039
HinP1I GCGC 7 cut(s) 294, 509, 937, 1610, 1894, 2037, 2039
HincII GTYRAC 2 cut(s) 16, 408
HindII GTYRAC 2 cut(s) 16, 408
HindIII AAGCTT 2 cut(s) 917, 1423
HinfI GANTC 9 cut(s) 152, 314, 359, 482, 733, 954, 1489, 1604, 1773
HpaII CCGG 7 cut(s) 27, 215, 288, 398, 1982, 2008, 2057
HphI GGTGA 9 cut(s) 316, 661, 730, 736, 812, 880, 1018, 1996, 2060
Hpy166II GTNNAC 3 cut(s) 16, 408, 1886
Hpy188III TCNNGA 7 cut(s) 76, 215, 424, 1172, 1476, 1622, 1712
Hpy8I GTNNAC 3 cut(s) 16, 408, 1886
Hpy99I CGWCG 4 cut(s) 17, 128, 152, 368
HpyAV CCTTC 5 cut(s) 263, 709, 715, 1845, 2028
HpyCH4III ACNGT 8 cut(s) 13, 497, 610, 812, 975, 1387, 1436, 1863
HpyCH4IV ACGT 2 cut(s) 123, 1285
HpyF10VI GCNNNNNNNGC 7 cut(s) 95, 1207, 1352, 1616, 1838, 1902, 2047
HpyF3I CTNAG 8 cut(s) 1112, 1173, 1181, 1202, 1251, 1271, 1753, 2046
HpySE526I ACGT 2 cut(s) 123, 1285
Hsp92I GRCGYC 4 cut(s) 123, 274, 294, 420
Hsp92II CATG 7 cut(s) 43, 965, 1570, 1742, 1793, 1943, 2036
HspAI GCGC 7 cut(s) 294, 509, 937, 1610, 1894, 2037, 2039
KasI GGCGCC 1 cut(s) 293
Kpn2I TCCGGA 1 cut(s) 214
KpnI GGTACC 1 cut(s) 1683
Kzo9I GATC 7 cut(s) 6, 130, 217, 904, 1649, 1868, 1978
LmnI GCTCC 8 cut(s) 49, 57, 595, 847, 1345, 1624, 1673, 1735
Lsp1109I GCAGC 6 cut(s) 784, 1093, 1134, 1210, 1339, 1828
LweI GCATC 9 cut(s) 496, 517, 934, 1024, 1118, 1200, 1336, 1547, 1772
MaeI CTAG 3 cut(s) 539, 615, 1089
MaeII ACGT 2 cut(s) 123, 1285
MaeIII GTNAC 6 cut(s) 491, 604, 610, 636, 1024, 1792
MalI GATC 7 cut(s) 8, 132, 219, 906, 1651, 1870, 1980
MbiI CCGCTC 1 cut(s) 850
MboI GATC 7 cut(s) 6, 130, 217, 904, 1649, 1868, 1978
MboII GAAGA 1 cut(s) 209
MfeI CAATTG 3 cut(s) 278, 299, 1758
MflI RGATCY 1 cut(s) 1978
MhlI GDGCHC 4 cut(s) 54, 171, 1670, 1993
MlsI TGGCCA 1 cut(s) 2031
MluCI AATT 8 cut(s) 245, 250, 262, 278, 299, 353, 853, 1758
MluNI TGGCCA 1 cut(s) 2031
Mly113I GGCGCC 1 cut(s) 294
MlyI GAGTC 3 cut(s) 727, 1483, 1767
Mox20I TGGCCA 1 cut(s) 2031
Mph1103I ATGCAT 2 cut(s) 967, 1133
MroI TCCGGA 1 cut(s) 214
MscI TGGCCA 1 cut(s) 2031
MseI TTAA 6 cut(s) 513, 669, 1005, 1016, 1071, 1629
MslI CAYNNNNRTG 1 cut(s) 556
Msp20I TGGCCA 1 cut(s) 2031
MspA1I CMGCKG 1 cut(s) 1201
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 7 cut(s) 27, 215, 288, 398, 1982, 2008, 2057
MspR9I CCNGG 3 cut(s) 1245, 1677, 2057
MunI CAATTG 3 cut(s) 278, 299, 1758
MvaI CCWGG 2 cut(s) 1245, 1677
MvnI CGCG 1 cut(s) 2039
MwoI GCNNNNNNNGC 7 cut(s) 95, 1207, 1352, 1616, 1838, 1902, 2047
NarI GGCGCC 1 cut(s) 294
NciI CCSGG 1 cut(s) 2057
NcoI CCATGG 1 cut(s) 2032
NdeI CATATG 1 cut(s) 1654
NdeII GATC 7 cut(s) 6, 130, 217, 904, 1649, 1868, 1978
NlaIII CATG 7 cut(s) 43, 965, 1570, 1742, 1793, 1943, 2036
NmuCI GTSAC 3 cut(s) 491, 604, 1024
NsbI TGCGCA 1 cut(s) 1895
NsiI ATGCAT 2 cut(s) 967, 1133
NspV TTCGAA 1 cut(s) 308
PaeR7I CTCGAG 1 cut(s) 1710
PalAI GGCGCGCC 1 cut(s) 2037
PauI GCGCGC 1 cut(s) 2037
PceI AGGCCT 1 cut(s) 1139
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PfeI GAWTC 6 cut(s) 152, 314, 359, 482, 954, 1604
PinAI ACCGGT 1 cut(s) 2007
PkrI GCNGC 7 cut(s) 88, 774, 1108, 1149, 1200, 1354, 1843
PleI GAGTC 3 cut(s) 727, 1483, 1767
PluTI GGCGCC 1 cut(s) 297
PpsI GAGTC 3 cut(s) 727, 1483, 1767
PshBI ATTAAT 1 cut(s) 1629
Psp124BI GAGCTC 2 cut(s) 54, 171
Psp6I CCWGG 2 cut(s) 1243, 1675
PspFI CCCAGC 2 cut(s) 530, 1689
PspGI CCWGG 2 cut(s) 1243, 1675
PspPI GGNCC 4 cut(s) 1057, 1247, 1376, 1936
PsuI RGATCY 1 cut(s) 1978
PteI GCGCGC 1 cut(s) 2037
PvuII CAGCTG 1 cut(s) 1201
RsaI GTAC 8 cut(s) 996, 1288, 1389, 1398, 1681, 1687, 1743, 1885
RsaNI GTAC 8 cut(s) 995, 1287, 1388, 1397, 1680, 1686, 1742, 1884
RseI CAYNNNNRTG 1 cut(s) 556
SacI GAGCTC 2 cut(s) 54, 171
SalI GTCGAC 1 cut(s) 14
SaqAI TTAA 6 cut(s) 513, 669, 1005, 1016, 1071, 1629
SatI GCNGC 7 cut(s) 87, 773, 1107, 1148, 1199, 1353, 1842
Sau3AI GATC 7 cut(s) 6, 130, 217, 904, 1649, 1868, 1978
Sau96I GGNCC 4 cut(s) 1057, 1247, 1376, 1936
SchI GAGTC 3 cut(s) 727, 1483, 1767
ScrFI CCNGG 3 cut(s) 1245, 1677, 2057
SduI GDGCHC 4 cut(s) 54, 171, 1670, 1993
SfaNI GCATC 9 cut(s) 496, 517, 934, 1024, 1118, 1200, 1336, 1547, 1772
SfoI GGCGCC 1 cut(s) 295
Sfr274I CTCGAG 1 cut(s) 1710
SfuI TTCGAA 1 cut(s) 308
SgsI GGCGCGCC 1 cut(s) 2037
SinI GGWCC 2 cut(s) 1376, 1936
SlaI CTCGAG 1 cut(s) 1710
SmiMI CAYNNNNRTG 1 cut(s) 556
SmlI CTYRAG 4 cut(s) 74, 512, 545, 1710
SmoI CTYRAG 4 cut(s) 74, 512, 545, 1710
Sse9I AATT 8 cut(s) 245, 250, 262, 278, 299, 353, 853, 1758
SseBI AGGCCT 1 cut(s) 1139
SsiI CCGC 9 cut(s) 47, 55, 87, 327, 845, 850, 1262, 1295, 1464
SspDI GGCGCC 1 cut(s) 293
SspI AATATT 1 cut(s) 1166
SspMI CTAG 3 cut(s) 539, 615, 1089
SstI GAGCTC 2 cut(s) 54, 171
StuI AGGCCT 1 cut(s) 1139
StyD4I CCNGG 3 cut(s) 1243, 1675, 2055
StyI CCWWGG 1 cut(s) 2032
TaaI ACNGT 8 cut(s) 13, 497, 610, 812, 975, 1387, 1436, 1863
TaiI ACGT 2 cut(s) 126, 1288
TasI AATT 8 cut(s) 245, 250, 262, 278, 299, 353, 853, 1758
TatI WGTACW 2 cut(s) 1396, 1741
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 6 cut(s) 152, 314, 359, 482, 954, 1604
Tru1I TTAA 6 cut(s) 513, 669, 1005, 1016, 1071, 1629
Tru9I TTAA 6 cut(s) 513, 669, 1005, 1016, 1071, 1629
TscAI CASTG 6 cut(s) 613, 669, 699, 817, 2019, 2032
TseFI GTSAC 3 cut(s) 491, 604, 1024
TseI GCWGC 6 cut(s) 772, 1106, 1147, 1198, 1352, 1841
Tsp45I GTSAC 3 cut(s) 491, 604, 1024
TspDTI ATGAA 1 cut(s) 1555
TspGWI ACGGA 2 cut(s) 352, 1722
TspRI CASTG 6 cut(s) 613, 669, 699, 817, 2019, 2032
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 2 cut(s) 1376, 1936
VspI ATTAAT 1 cut(s) 1629
XapI RAATTY 1 cut(s) 250
XhoI CTCGAG 1 cut(s) 1710
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 3 cut(s) 539, 615, 1089
ZraI GACGTC 1 cut(s) 124
Zsp2I ATGCAT 2 cut(s) 967, 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.