pycom05g00120

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013126
Physical Location & Seq
Reverse (-)
154212 .. 157248
3037 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 690 bp
ATGTACCAATTCTTGGTGTTTTGCGTCCCCATCCTGTTCACTTTTATCCTCCTTTTCGTCTTCTACTTGTTCTATTTTCGCCCTCGACGCCTCAATTGGTCCTCCCGCGGAACGACAACATCTCTTCCGACTCCAGGGAATCACACTCACATATTTGCCACTGTTGAGTTGGGGTTGAAGAAAGAGCTCAGGGAGATGCTGCCCATTATTGTATATAAGGAGAGCTTCTCCGTCAGAGATACACAATGCTCAGTATGCCTGGGTGACTATGTAGCGGAGGATAGACTTCAACAGATACCATCCTGTGGCCATACATTTCACATTGATTGCATTGACAGCTGGCTTTCTACCCACAGCACTTGTCCACTGTGCCGCTCATCCCTCCTTTCTGCCCATAAATATCAAAATGAATCGCCTGATGTACTAGTGGAACCAAGTCATGAATCTTCTGTTGCGGTGAATAGGAGTGTAACATCTCTTCTACAGATGCCTCAGGCTTGTGAAGAAACAAAAGCTACAGAGTTTTCTGGGTTGAGGAATGGAGAACCAACAAACGCCCAAAACAGTTCCAAAGAAGATGCAAGTTCTGAGTGGGTTGATTGCAAGAGAGGATTGAGGGATGCAGGAAATGCAACTCAAGTGCATCAGCATACTGGTGGATCATCTGATGGACTTGAATTGAGGCATTAG

Protein Analysis

230

Amino Acids

25.78

Weight (kDa)

6.04

Isoelectric Point (pI)

56.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 82 - 125 3e-14 Ring finger domain
zf-RING_11 PF17123 82 - 111 9.7e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 82 - 124 1.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 83 - 124 3.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 83 - 122 2.2e-06 RING-type zinc-finger
zf-rbx1 PF12678 98 - 125 7.5e-09 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33480 AT4G10150 AT4G10160
fragaria_vesca FvH4_2g00300
malus_domestica MD05G1006000.v1.1
prunus_persica Prupe.8G002900_v2.0.a1
pyrus_communis pycom05g00120
rosa_chinensis RchiOBHm_Chr6g0249581
rosa_laevigata RLG00000015560
rosa_multiflora Rmu_sc0006936.1_g000008 Rmu_sc0013555.1_g000005
rosa_roxburghii Rroxscaffold_7G00217530
rosa_rugosa Rorug05G0493800
rosa_samantha Rh6AG003600 Rh6BG006600 Rh6CG002700 Rh6DG002500
rosa_wichuraiana Rw6G000400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 13, 305
AccBSI CCGCTC 1 cut(s) 375
AccII CGCG 1 cut(s) 108
AciI CCGC 5 cut(s) 106, 108, 275, 373, 455
AclWI GGATC 1 cut(s) 667
AcoI YGGCCR 1 cut(s) 307
AcyI GRCGYC 1 cut(s) 88
AfaI GTAC 2 cut(s) 5, 423
AfiI CCNNNNNNNGG 3 cut(s) 13, 134, 305
AgsI TTSAA 3 cut(s) 178, 290, 677
AhlI ACTAGT 1 cut(s) 424
AjnI CCWGG 2 cut(s) 133, 258
AluBI AGCT 4 cut(s) 187, 225, 339, 515
AluI AGCT 4 cut(s) 187, 225, 339, 515
Alw21I GWGCWC 1 cut(s) 189
AlwI GGATC 1 cut(s) 667
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 1 cut(s) 199
AspS9I GGNCC 1 cut(s) 99
AsuHPI GGTGA 2 cut(s) 275, 469
AvaII GGWCC 1 cut(s) 99
AxyI CCTNAGG 1 cut(s) 492
BalI TGGCCA 1 cut(s) 309
BanII GRGCYC 1 cut(s) 189
BarI GAAGNNNNNNTAC 2 cut(s) 462, 494
BbsI GAAGAC 1 cut(s) 52
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 1 cut(s) 186
BccI CCATC 3 cut(s) 38, 307, 662
BciT130I CCWGG 2 cut(s) 135, 260
BcuI ACTAGT 1 cut(s) 424
BfaI CTAG 1 cut(s) 425
BfmI CTRYAG 2 cut(s) 482, 516
BisI GCNGC 2 cut(s) 200, 373
BlsI GCNGC 2 cut(s) 201, 374
Bme1390I CCNGG 2 cut(s) 135, 260
Bme18I GGWCC 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 99
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 2 cut(s) 135, 260
BmsI GCATC 5 cut(s) 186, 477, 568, 610, 652
BpiI GAAGAC 1 cut(s) 52
BplI GAGNNNNNCTC 2 cut(s) 212, 244
BpmI CTGGAG 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 621
BsaHI GRCGYC 1 cut(s) 88
BsaJI CCNNGG 3 cut(s) 106, 134, 259
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 134, 305
Bse1I ACTGG 1 cut(s) 658
Bse21I CCTNAGG 1 cut(s) 492
BseBI CCWGG 2 cut(s) 135, 260
BseDI CCNNGG 3 cut(s) 106, 134, 259
BseGI GGATG 4 cut(s) 30, 299, 377, 625
BseLI CCNNNNNNNGG 3 cut(s) 13, 134, 305
BseMII CTCAG 4 cut(s) 202, 264, 506, 579
BseNI ACTGG 1 cut(s) 658
BseXI GCAGC 1 cut(s) 186
Bsh1236I CGCG 1 cut(s) 108
BshFI GGCC 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 189
BslFI GGGAC 1 cut(s) 11
BslI CCNNNNNNNGG 3 cut(s) 13, 134, 305
BsmFI GGGAC 1 cut(s) 11
BsnI GGCC 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 1 cut(s) 659
BspACI CCGC 5 cut(s) 106, 108, 275, 373, 455
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 4 cut(s) 201, 263, 505, 580
BspFNI CGCG 1 cut(s) 108
BspHI TCATGA 1 cut(s) 439
BspLI GGNNCC 1 cut(s) 432
BspPI GGATC 1 cut(s) 667
BsrBI CCGCTC 1 cut(s) 375
BsrI ACTGG 1 cut(s) 658
BssECI CCNNGG 3 cut(s) 106, 134, 259
BssMI GATC 1 cut(s) 659
BssNI GRCGYC 1 cut(s) 88
Bst2UI CCWGG 2 cut(s) 135, 260
Bst4CI ACNGT 3 cut(s) 163, 369, 566
Bst6I CTCTTC 2 cut(s) 129, 483
BstACI GRCGYC 1 cut(s) 88
BstAPI GCANNNNNTGC 1 cut(s) 629
BstC8I GCNNGC 1 cut(s) 341
BstDEI CTNAG 4 cut(s) 188, 250, 492, 588
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 4 cut(s) 30, 299, 377, 625
BstFNI CGCG 1 cut(s) 108
BstKTI GATC 1 cut(s) 662
BstMBI GATC 1 cut(s) 659
BstMWI GCNNNNNNNGC 4 cut(s) 87, 255, 336, 629
BstNI CCWGG 2 cut(s) 135, 260
BstSCI CCNGG 2 cut(s) 133, 258
BstSFI CTRYAG 2 cut(s) 482, 516
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 1 cut(s) 186
BstV2I GAAGAC 1 cut(s) 52
Bsu36I CCTNAGG 1 cut(s) 492
BsuRI GGCC 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 4 cut(s) 30, 299, 377, 625
BtsIMutI CAGTG 2 cut(s) 159, 365
Cac8I GCNNGC 1 cut(s) 341
CciI TCATGA 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 99
Cfr42I CCGCGG 1 cut(s) 109
CseI GACGC 2 cut(s) 13, 96
Csp6I GTAC 2 cut(s) 4, 422
CspCI CAANNNNNGTGG 2 cut(s) 341, 376
CviAII CATG 1 cut(s) 440
CviJI RGCY 7 cut(s) 187, 225, 309, 339, 343, 497, 515
CviKI_1 RGCY 7 cut(s) 187, 225, 309, 339, 343, 497, 515
CviQI GTAC 2 cut(s) 4, 422
DdeI CTNAG 4 cut(s) 188, 250, 492, 588
DpnI GATC 1 cut(s) 661
DpnII GATC 1 cut(s) 659
EaeI YGGCCR 1 cut(s) 307
Eam1104I CTCTTC 2 cut(s) 129, 483
EarI CTCTTC 2 cut(s) 129, 483
Ecl136II GAGCTC 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 187
Eco81I CCTNAGG 1 cut(s) 492
EcoICRI GAGCTC 1 cut(s) 187
EcoRII CCWGG 2 cut(s) 133, 258
EcoT38I GRGCYC 1 cut(s) 189
FaeI CATG 1 cut(s) 443
FaiI YATR 9 cut(s) 152, 214, 216, 256, 270, 312, 396, 441, 651
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FaqI GGGAC 1 cut(s) 11
FatI CATG 1 cut(s) 439
FauI CCCGC 1 cut(s) 113
Fnu4HI GCNGC 2 cut(s) 200, 373
FokI GGATG 4 cut(s) 17, 286, 364, 632
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 2 cut(s) 200, 373
FspBI CTAG 1 cut(s) 425
GluI GCNGC 2 cut(s) 200, 373
GsuI CTGGAG 1 cut(s) 117
HaeIII GGCC 1 cut(s) 309
HgaI GACGC 2 cut(s) 13, 96
Hin1I GRCGYC 1 cut(s) 88
Hin1II CATG 1 cut(s) 443
HinfI GANTC 4 cut(s) 130, 139, 410, 443
HphI GGTGA 2 cut(s) 275, 469
Hpy166II GTNNAC 2 cut(s) 39, 365
Hpy188I TCNGA 4 cut(s) 129, 236, 589, 667
Hpy188III TCNNGA 1 cut(s) 440
Hpy8I GTNNAC 2 cut(s) 39, 365
Hpy99I CGWCG 1 cut(s) 90
HpyCH4III ACNGT 3 cut(s) 163, 369, 566
HpyCH4V TGCA 6 cut(s) 330, 581, 603, 623, 632, 643
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 255, 336, 629
HpyF3I CTNAG 4 cut(s) 188, 250, 492, 588
Hsp92I GRCGYC 1 cut(s) 88
Hsp92II CATG 1 cut(s) 443
KspI CCGCGG 1 cut(s) 109
Kzo9I GATC 1 cut(s) 659
Lsp1109I GCAGC 1 cut(s) 186
LweI GCATC 5 cut(s) 186, 477, 568, 610, 652
MaeI CTAG 1 cut(s) 425
MaeIII GTNAC 2 cut(s) 263, 469
MalI GATC 1 cut(s) 661
MbiI CCGCTC 1 cut(s) 375
MboI GATC 1 cut(s) 659
MboII GAAGA 7 cut(s) 52, 116, 190, 438, 470, 515, 587
MfeI CAATTG 1 cut(s) 94
MhlI GDGCHC 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 3 cut(s) 8, 94, 677
MluNI TGGCCA 1 cut(s) 309
MlyI GAGTC 1 cut(s) 124
MmeI TCCRAC 1 cut(s) 152
Mox20I TGGCCA 1 cut(s) 309
MscI TGGCCA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 654
Msp20I TGGCCA 1 cut(s) 309
MspA1I CMGCKG 2 cut(s) 108, 339
MspR9I CCNGG 2 cut(s) 135, 260
MunI CAATTG 1 cut(s) 94
MvaI CCWGG 2 cut(s) 135, 260
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 4 cut(s) 87, 255, 336, 629
NdeII GATC 1 cut(s) 659
NlaIII CATG 1 cut(s) 443
NlaIV GGNNCC 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 263
PagI TCATGA 1 cut(s) 439
PfeI GAWTC 3 cut(s) 139, 410, 443
PflMI CCANNNNNTGG 2 cut(s) 13, 305
PkrI GCNGC 2 cut(s) 201, 374
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp124BI GAGCTC 1 cut(s) 189
Psp6I CCWGG 2 cut(s) 133, 258
PspGI CCWGG 2 cut(s) 133, 258
PspN4I GGNNCC 1 cut(s) 432
PspPI GGNCC 1 cut(s) 99
PvuII CAGCTG 1 cut(s) 339
RsaI GTAC 2 cut(s) 5, 423
RsaNI GTAC 2 cut(s) 4, 422
RseI CAYNNNNRTG 1 cut(s) 654
SacI GAGCTC 1 cut(s) 189
SacII CCGCGG 1 cut(s) 109
SatI GCNGC 2 cut(s) 200, 373
Sau3AI GATC 1 cut(s) 659
Sau96I GGNCC 1 cut(s) 99
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 2 cut(s) 135, 260
SduI GDGCHC 1 cut(s) 189
SetI ASST 4 cut(s) 189, 227, 341, 517
SfaNI GCATC 5 cut(s) 186, 477, 568, 610, 652
SfcI CTRYAG 2 cut(s) 482, 516
Sfr303I CCGCGG 1 cut(s) 109
SgrBI CCGCGG 1 cut(s) 109
SinI GGWCC 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 1 cut(s) 636
SmoI CTYRAG 1 cut(s) 636
SpeI ACTAGT 1 cut(s) 424
Sse9I AATT 3 cut(s) 8, 94, 677
SsiI CCGC 5 cut(s) 106, 108, 275, 373, 455
SspMI CTAG 1 cut(s) 425
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 133, 258
TaaI ACNGT 3 cut(s) 163, 369, 566
TaqI TCGA 1 cut(s) 85
TasI AATT 3 cut(s) 8, 94, 677
TatI WGTACW 1 cut(s) 421
TauI GCSGC 1 cut(s) 375
TfiI GAWTC 3 cut(s) 139, 410, 443
TscAI CASTG 2 cut(s) 166, 372
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 1 cut(s) 199
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 2 cut(s) 423, 456
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 2 cut(s) 166, 372
Van91I CCANNNNNTGG 2 cut(s) 13, 305
VpaK11BI GGWCC 1 cut(s) 99
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XspI CTAG 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.