pycom05g00200

Sad1 / UNC-like C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003815
Physical Location & Seq
Reverse (-)
241483 .. 245440
3958 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1824 bp
ATGCAAAGATCACGTAGTGCTCTTTTGAACAGAAGAGCTTTAGCGATCAGCGGAAGAAGCCACTTGTATAAGGTGTCTCTGTCTTTCGTTTTTGTCCTCTGGGGGCTTGTCTTTCTATTCAGCTCATGGTTCAGTTGTGGACACGGATACAAAGATGGATCCACTGTATCTCCAGTTGGTATATCAACTTGGGACGAAGCTAAGCTGGACCGTGATGAACACTATGATTCTGTAAATATACAAAAAGAAACTGATTTTGTCTACTCTTCTGGAGGTGAATGCACAAATGGGATTGAAACTGGTGGTTTAAAGGGTGAGTTATCTGCTACGGAAAGAAGTAAACACCATGTCTCAGCTGAAGGAAGTCCACATCATGCCTCAGCTGAAGGAAGTATGTTTCATGACTCAGCTGTGGGTGGGCAACCTGAGGTGGTGAATGCTGGTTCAGGTGCAAAACTTGAAAATGATGCTCTAAAGAATGGTCGCTTGCCACGTGCTGTGCCTCGTGGTCTCGATGAATTCAAAAGCAAAACATTTAGTTCCAAAAGTAAATCTGGAAATGGTCAGGCTGAAGGCATAAAACATAGGGTGGAGCCTGGTGGTGCAGAGTACAATTATGCGTCAGCCGCAAAGGGAGCCAAGGTCTTGGCTTTTAACAAGGAAGCTAAGGGTGCCTCTAATATCTTAGGCAAAGACAAAGACAAGTACCTTCGCAATCCATGTTCTGCAGAAGAAAAGTTTGTCGATATAGAGCTTTCCGAAGAAACCTTAGTAGATTCAATTGAAATAGCTAATCTTGAGCACTATTCATCTAATTTAAAAGATTTTGAGGTGCTTGGCAGTTTGGCTTATCCAACTAATGAATGGGTCTTTCTTGGGAATGGTACTGCTGCAAATAATAAACTTGTACAAAGGTTTGTTCTTCAGCAACCCAAGTGGGTGAGATATATTAAGTTGAAACTTTTGAGCCATTATGGTTCAGAATTCTACTGCACGCTTAGTAGTATTGAACTCTATGGAGTGGATGCTGTTGAGCGAATGCTTGAGGATTTGATTTCTGTTGAAGGCGGCTCGTTTGTTACTTCGGGAGCAGCAGCTGACCAGAAACCTGTGCCCTCCCACCCGGACTCCCCTGAGGGTGAGGAATTGTGTCCTGATATGGTTAAGGAATTGGAACCCCAAGATGCAGCTGGAGGGTCTGAAGTGAATAATAATATGATGAATAGTGAAGTGCCTGATCCTGTCAAAGAAGTTCATCAACAAGTTAATAGGATGCCTGGGGACACTGTTCTAAAAATTTTAATGCAGAAAGTTCGTTCATTAGATTTTAGTTTATCAGTTCTGGAGCGATACCTGGAGGAATCAACTTCAAAATATGGCAGTATTTTCGGAGAATTTGACAAAGATTTGGGAGAGAAAGATATAGATTTAAAGAAGATCAGAGAAGACATAAGAAATCTTGTTCAAAGCCAGCAAGTCATTGCTAATGATGTTGACAATCTTATATCTTGGCAGTCCCTAGTTACCATGCAATTGGATAATCTAGTCAGGGACAATGCTATCCTCAGATCTGAGGTTGAAGGGGTGAGGGAGAAGCAGGCATCTGTAGATAATAAGGGTGTCATAATATTTCTTATATGCATAATTTTTTCATTGTTAGCTCTTGTGAGGCTTTTCACAGAAATGGCAGTTAGTGTTTGTATGATGGCATCGAGTGTTGATACGACGACAGAAAAGTCGAGGAAGTTTTGTTGGACGAGTTCTTCGTCCTGGATTTTTTTGCTTGTGAGCTGTATCCTTGTCCTATTTATATTATCATTATAA

Protein Analysis

608

Amino Acids

66.94

Weight (kDa)

5.33

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sad1_UNC PF07738 219 - 341 1.7e-32 Sad1 / UNC-like C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1822
AasI GACNNNNNNGTC 1 cut(s) 1735
AccB1I GGYRCC 1 cut(s) 671
AccI GTMKAC 1 cut(s) 261
AciI CCGC 3 cut(s) 51, 627, 1068
AclWI GGATC 3 cut(s) 153, 166, 1232
AcsI RAATTY 4 cut(s) 518, 983, 1296, 1394
AcuI CTGAAG 5 cut(s) 378, 405, 591, 908, 1221
AcvI CACGTG 1 cut(s) 494
AdeI CACNNNGTG 1 cut(s) 17
AfaI GTAC 4 cut(s) 611, 707, 886, 909
AjnI CCWGG 4 cut(s) 595, 1276, 1353, 1769
Alw21I GWGCWC 2 cut(s) 22, 804
Alw26I GTCTC 3 cut(s) 81, 355, 515
AlwI GGATC 3 cut(s) 153, 166, 1232
AlwNI CAGNNNCTG 1 cut(s) 1097
ApeKI GCWGC 4 cut(s) 890, 1091, 1094, 1187
ApoI RAATTY 4 cut(s) 518, 983, 1296, 1394
ArsI GACNNNNNNTTYG 2 cut(s) 1747, 1779
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 1 cut(s) 1124
AsuHPI GGTGA 6 cut(s) 287, 326, 445, 952, 1151, 1597
AvaII GGWCC 1 cut(s) 208
AxyI CCTNAGG 2 cut(s) 426, 1134
BaeGI GKGCMC 1 cut(s) 1116
BamHI GGATCC 1 cut(s) 158
BanI GGYRCC 1 cut(s) 671
BauI CACGAG 1 cut(s) 504
BbrPI CACGTG 1 cut(s) 494
BbsI GAAGAC 1 cut(s) 1452
Bbv12I GWGCWC 2 cut(s) 22, 804
BbvCI CCTCAGC 1 cut(s) 379
BbvI GCAGC 4 cut(s) 877, 1103, 1106, 1199
BccI CCATC 2 cut(s) 149, 1699
BcgI CGANNNNNNTGC 4 cut(s) 1295, 1329, 1369, 1403
BciT130I CCWGG 4 cut(s) 597, 1278, 1355, 1771
BciVI GTATCC 2 cut(s) 140, 1805
BcnI CCSGG 1 cut(s) 1124
BcoDI GTCTC 3 cut(s) 81, 355, 515
BfaI CTAG 2 cut(s) 1520, 1544
BfmI CTRYAG 2 cut(s) 726, 1605
BfuI GTATCC 2 cut(s) 140, 1805
BglII AGATCT 1 cut(s) 1568
BisI GCNGC 6 cut(s) 627, 891, 1069, 1092, 1095, 1188
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 6 cut(s) 628, 892, 1070, 1093, 1096, 1189
Bme1390I CCNGG 5 cut(s) 597, 1124, 1278, 1355, 1771
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 5 cut(s) 160, 594, 637, 673, 1176
BmrFI CCNGG 5 cut(s) 597, 1124, 1278, 1355, 1771
BmsI GCATC 6 cut(s) 457, 1015, 1174, 1263, 1610, 1718
BpiI GAAGAC 1 cut(s) 1452
BpmI CTGGAG 5 cut(s) 156, 291, 1212, 1364, 1376
Bpu10I CCTNAGC 2 cut(s) 379, 666
Bpu1102I GCTNAGC 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 818, 1064
BpuMI CCSGG 1 cut(s) 1124
BsaAI YACGTR 2 cut(s) 14, 494
BsaI GGTCTC 1 cut(s) 515
BsaJI CCNNGG 2 cut(s) 639, 1277
BsaXI ACNNNNNCTCC 6 cut(s) 154, 184, 627, 657, 1112, 1142
Bse1I ACTGG 2 cut(s) 173, 304
Bse21I CCTNAGG 2 cut(s) 426, 1134
Bse3DI GCAATG 1 cut(s) 1479
BseBI CCWGG 4 cut(s) 597, 1278, 1355, 1771
BseDI CCNNGG 2 cut(s) 639, 1277
BseGI GGATG 2 cut(s) 1030, 1278
BseMI GCAATG 1 cut(s) 1479
BseMII CTCAG 7 cut(s) 366, 393, 417, 420, 1125, 1563, 1579
BseNI ACTGG 2 cut(s) 173, 304
BseSI GKGCMC 1 cut(s) 1116
BseXI GCAGC 4 cut(s) 877, 1103, 1106, 1199
BsgI GTGCAG 2 cut(s) 624, 976
BshNI GGYRCC 1 cut(s) 671
BsiHKAI GWGCWC 2 cut(s) 22, 804
BsiSI CCGG 1 cut(s) 1124
BslFI GGGAC 4 cut(s) 206, 1295, 1501, 1565
BsmAI GTCTC 3 cut(s) 81, 355, 515
BsmFI GGGAC 4 cut(s) 206, 1295, 1501, 1565
BsmI GAATGC 3 cut(s) 284, 442, 1044
Bso31I GGTCTC 1 cut(s) 515
Bsp1286I GDGCHC 3 cut(s) 22, 804, 1116
Bsp1407I TGTACA 1 cut(s) 907
Bsp143I GATC 6 cut(s) 8, 45, 158, 1237, 1437, 1568
Bsp1720I GCTNAGC 1 cut(s) 201
BspACI CCGC 3 cut(s) 51, 627, 1068
BspCNI CTCAG 7 cut(s) 365, 392, 418, 419, 1126, 1564, 1578
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 5 cut(s) 160, 594, 637, 673, 1176
BspMAI CTGCAG 1 cut(s) 730
BspPI GGATC 3 cut(s) 153, 166, 1232
BspQI GCTCTTC 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 671
BspTNI GGTCTC 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 1479
BsrGI TGTACA 1 cut(s) 907
BsrI ACTGG 2 cut(s) 173, 304
BssECI CCNNGG 2 cut(s) 639, 1277
BssMI GATC 6 cut(s) 8, 45, 158, 1237, 1437, 1568
BssSI CACGAG 1 cut(s) 504
BssT1I CCWWGG 1 cut(s) 639
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 4 cut(s) 597, 1278, 1355, 1771
Bst4CI ACNGT 3 cut(s) 166, 212, 1288
Bst6I CTCTTC 2 cut(s) 28, 271
BstAUI TGTACA 1 cut(s) 907
BstBAI YACGTR 2 cut(s) 14, 494
BstC8I GCNNGC 4 cut(s) 488, 995, 1472, 1599
BstF5I GGATG 2 cut(s) 1030, 1278
BstKTI GATC 6 cut(s) 11, 48, 161, 1240, 1440, 1571
BstMAI GTCTC 3 cut(s) 81, 355, 515
BstMBI GATC 6 cut(s) 8, 45, 158, 1237, 1437, 1568
BstMWI GCNNNNNNNGC 4 cut(s) 57, 626, 635, 671
BstNI CCWGG 4 cut(s) 597, 1278, 1355, 1771
BstSCI CCNGG 5 cut(s) 595, 1122, 1276, 1353, 1769
BstSFI CTRYAG 2 cut(s) 726, 1605
BstSLI GKGCMC 1 cut(s) 1116
BstV1I GCAGC 4 cut(s) 877, 1103, 1106, 1199
BstV2I GAAGAC 1 cut(s) 1452
BstX2I RGATCY 2 cut(s) 158, 1568
BstXI CCANNNNNNTGG 2 cut(s) 646, 1534
BstYI RGATCY 2 cut(s) 158, 1568
Bsu36I CCTNAGG 2 cut(s) 426, 1134
BsuI GTATCC 2 cut(s) 140, 1805
BtsCI GGATG 2 cut(s) 1030, 1278
BtsIMutI CAGTG 2 cut(s) 162, 1284
Cac8I GCNNGC 4 cut(s) 488, 995, 1472, 1599
CaiI CAGNNNCTG 1 cut(s) 1097
CciI TCATGA 1 cut(s) 400
Cfr13I GGNCC 1 cut(s) 208
CseI GACGC 1 cut(s) 609
Csp6I GTAC 4 cut(s) 610, 706, 885, 908
CspCI CAANNNNNGTGG 2 cut(s) 917, 952
CviAII CATG 6 cut(s) 126, 347, 374, 401, 720, 1528
CviQI GTAC 4 cut(s) 610, 706, 885, 908
DpnI GATC 6 cut(s) 10, 47, 160, 1239, 1439, 1570
DpnII GATC 6 cut(s) 8, 45, 158, 1237, 1437, 1568
DraI TTTAAA 3 cut(s) 309, 819, 1431
DraIII CACNNNGTG 1 cut(s) 17
DrdI GACNNNNNNGTC 1 cut(s) 1735
DseDI GACNNNNNNGTC 1 cut(s) 1735
Eam1104I CTCTTC 2 cut(s) 28, 271
EarI CTCTTC 2 cut(s) 28, 271
Eco130I CCWWGG 1 cut(s) 639
Eco31I GGTCTC 1 cut(s) 515
Eco47I GGWCC 1 cut(s) 208
Eco57I CTGAAG 5 cut(s) 378, 405, 591, 908, 1221
Eco72I CACGTG 1 cut(s) 494
Eco81I CCTNAGG 2 cut(s) 426, 1134
EcoRI GAATTC 2 cut(s) 518, 983
EcoRII CCWGG 4 cut(s) 595, 1276, 1353, 1769
EcoT14I CCWWGG 1 cut(s) 639
EcoT22I ATGCAT 1 cut(s) 1643
ErhI CCWWGG 1 cut(s) 639
FaeI CATG 6 cut(s) 129, 350, 377, 404, 723, 1531
FaqI GGGAC 4 cut(s) 206, 1295, 1501, 1565
FatI CATG 6 cut(s) 125, 346, 373, 400, 719, 1527
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 6 cut(s) 627, 891, 1069, 1092, 1095, 1188
FokI GGATG 2 cut(s) 1037, 1285
Fsp4HI GCNGC 6 cut(s) 627, 891, 1069, 1092, 1095, 1188
FspBI CTAG 2 cut(s) 1520, 1544
GluI GCNGC 6 cut(s) 627, 891, 1069, 1092, 1095, 1188
GsuI CTGGAG 5 cut(s) 156, 291, 1212, 1364, 1376
HapII CCGG 1 cut(s) 1124
HgaI GACGC 1 cut(s) 609
Hin1II CATG 6 cut(s) 129, 350, 377, 404, 723, 1531
HincII GTYRAC 1 cut(s) 1495
HindII GTYRAC 1 cut(s) 1495
HinfI GANTC 5 cut(s) 227, 404, 776, 1127, 1361
HpaII CCGG 1 cut(s) 1124
HphI GGTGA 6 cut(s) 287, 326, 445, 952, 1151, 1597
Hpy166II GTNNAC 5 cut(s) 140, 262, 341, 368, 1495
Hpy188I TCNGA 7 cut(s) 760, 982, 1201, 1391, 1442, 1568, 1573
Hpy188III TCNNGA 8 cut(s) 270, 401, 512, 555, 797, 1086, 1154, 1343
Hpy8I GTNNAC 5 cut(s) 140, 262, 341, 368, 1495
Hpy99I CGWCG 1 cut(s) 1729
HpyAV CCTTC 6 cut(s) 353, 380, 566, 719, 1058, 1574
HpyCH4III ACNGT 3 cut(s) 166, 212, 1288
HpyCH4IV ACGT 2 cut(s) 13, 493
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 626, 635, 671
HpySE526I ACGT 2 cut(s) 13, 493
Hsp92II CATG 6 cut(s) 129, 350, 377, 404, 723, 1531
Kzo9I GATC 6 cut(s) 8, 45, 158, 1237, 1437, 1568
LguI GCTCTTC 1 cut(s) 28
LmnI GCTCC 4 cut(s) 592, 635, 1088, 1345
Lsp1109I GCAGC 4 cut(s) 877, 1103, 1106, 1199
LweI GCATC 6 cut(s) 457, 1015, 1174, 1263, 1610, 1718
MaeI CTAG 2 cut(s) 1520, 1544
MaeII ACGT 2 cut(s) 13, 493
MaeIII GTNAC 2 cut(s) 1078, 1522
MalI GATC 6 cut(s) 10, 47, 160, 1239, 1439, 1570
MboI GATC 6 cut(s) 8, 45, 158, 1237, 1437, 1568
MboII GAAGA 9 cut(s) 45, 66, 258, 743, 773, 914, 1447, 1457, 1755
MfeI CAATTG 2 cut(s) 780, 1532
MflI RGATCY 2 cut(s) 158, 1568
MhlI GDGCHC 3 cut(s) 22, 804, 1116
MlyI GAGTC 2 cut(s) 398, 1121
MmeI TCCRAC 2 cut(s) 878, 1733
Mph1103I ATGCAT 1 cut(s) 1643
MseI TTAA 8 cut(s) 308, 654, 818, 951, 1164, 1266, 1301, 1430
MslI CAYNNNNRTG 1 cut(s) 1682
MspA1I CMGCKG 6 cut(s) 51, 356, 383, 410, 1097, 1190
MspI CCGG 1 cut(s) 1124
MspR9I CCNGG 5 cut(s) 597, 1124, 1278, 1355, 1771
MunI CAATTG 2 cut(s) 780, 1532
Mva1269I GAATGC 3 cut(s) 284, 442, 1044
MvaI CCWGG 4 cut(s) 597, 1278, 1355, 1771
MwoI GCNNNNNNNGC 4 cut(s) 57, 626, 635, 671
NciI CCSGG 1 cut(s) 1124
NdeII GATC 6 cut(s) 8, 45, 158, 1237, 1437, 1568
NlaIII CATG 6 cut(s) 129, 350, 377, 404, 723, 1531
NlaIV GGNNCC 5 cut(s) 160, 594, 637, 673, 1176
NsiI ATGCAT 1 cut(s) 1643
PagI TCATGA 1 cut(s) 400
PciSI GCTCTTC 1 cut(s) 28
PcsI WCGNNNNNNNCGW 2 cut(s) 490, 1763
PctI GAATGC 3 cut(s) 284, 442, 1044
PfeI GAWTC 3 cut(s) 227, 776, 1361
PfoI TCCNGGA 1 cut(s) 1769
PkrI GCNGC 6 cut(s) 628, 892, 1070, 1093, 1096, 1189
PleI GAGTC 2 cut(s) 398, 1121
PmaCI CACGTG 1 cut(s) 494
PmlI CACGTG 1 cut(s) 494
PpsI GAGTC 2 cut(s) 398, 1121
Ppu21I YACGTR 2 cut(s) 14, 494
PsiI TTATAA 1 cut(s) 1822
Psp6I CCWGG 4 cut(s) 595, 1276, 1353, 1769
PspCI CACGTG 1 cut(s) 494
PspGI CCWGG 4 cut(s) 595, 1276, 1353, 1769
PspN4I GGNNCC 5 cut(s) 160, 594, 637, 673, 1176
PspPI GGNCC 1 cut(s) 208
PstI CTGCAG 1 cut(s) 730
PstNI CAGNNNCTG 1 cut(s) 1097
PsuI RGATCY 2 cut(s) 158, 1568
PvuII CAGCTG 5 cut(s) 356, 383, 410, 1097, 1190
RsaI GTAC 4 cut(s) 611, 707, 886, 909
RsaNI GTAC 4 cut(s) 610, 706, 885, 908
RseI CAYNNNNRTG 1 cut(s) 1682
SapI GCTCTTC 1 cut(s) 28
SaqAI TTAA 8 cut(s) 308, 654, 818, 951, 1164, 1266, 1301, 1430
SatI GCNGC 6 cut(s) 627, 891, 1069, 1092, 1095, 1188
Sau3AI GATC 6 cut(s) 8, 45, 158, 1237, 1437, 1568
Sau96I GGNCC 1 cut(s) 208
SchI GAGTC 2 cut(s) 398, 1121
ScrFI CCNGG 5 cut(s) 597, 1124, 1278, 1355, 1771
SduI GDGCHC 3 cut(s) 22, 804, 1116
SfaNI GCATC 6 cut(s) 457, 1015, 1174, 1263, 1610, 1718
SfcI CTRYAG 2 cut(s) 726, 1605
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 1682
SmlI CTYRAG 2 cut(s) 797, 1043
SmoI CTYRAG 2 cut(s) 797, 1043
SsiI CCGC 3 cut(s) 51, 627, 1068
SspI AATATT 1 cut(s) 1629
SspMI CTAG 2 cut(s) 1520, 1544
StyD4I CCNGG 5 cut(s) 595, 1122, 1276, 1353, 1769
StyI CCWWGG 1 cut(s) 639
TaaI ACNGT 3 cut(s) 166, 212, 1288
TaiI ACGT 2 cut(s) 16, 496
TaqI TCGA 4 cut(s) 513, 744, 1712, 1739
TatI WGTACW 2 cut(s) 609, 907
TauI GCSGC 2 cut(s) 629, 1071
TfiI GAWTC 3 cut(s) 227, 776, 1361
Tru1I TTAA 8 cut(s) 308, 654, 818, 951, 1164, 1266, 1301, 1430
Tru9I TTAA 8 cut(s) 308, 654, 818, 951, 1164, 1266, 1301, 1430
TscAI CASTG 2 cut(s) 169, 1291
TseI GCWGC 4 cut(s) 890, 1091, 1094, 1187
TspDTI ATGAA 9 cut(s) 231, 389, 531, 798, 876, 1235, 1244, 1308, 1641
TspGWI ACGGA 2 cut(s) 159, 344
TspRI CASTG 2 cut(s) 169, 1291
VpaK11BI GGWCC 1 cut(s) 208
XapI RAATTY 4 cut(s) 518, 983, 1296, 1394
XcmI CCANNNNNNNNNTGG 2 cut(s) 861, 1187
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 2 cut(s) 1520, 1544
Zsp2I ATGCAT 1 cut(s) 1643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.