pycom05g03290

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016258
Physical Location & Seq
Forward (+)
3818614 .. 3820106
1493 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1428 bp
ATGGAAGACATAGATGAGGAGGAGGAGCTTCTGAATCTTAGCCTTGCAATTGTTACAAGTTCAGGGAGGGAGAGATTTAAGAAGAGGAAGAGATCAAGAGATGATGGTATTGCTAATAGTATTTTGGTGAGCCCTCTAAACTCTTATGAAGGTTGTGAAGGGAAGATATTTAGGCTTCTTCAAATGAGGGAACAAATGCTAAAAAAACTAGACCTTCACAAGAAGAAAGGGGTTGCTTTTGATGATGAAGATGGAAAGGGCCTCCACCTGATCCACATGTTGCTCATAACAGCCACCGCAGTTAACGAAAACAATGTCGGCTCAGCCTTGGAGCATCTTACTGAGTTGTACAAAATTGTTTCCTTATGCGGTGACTCTGTCCAACGAGTAGTGGCTTATTTTGCAGACGGCTTGGCTGCAAGGCTTCTCACTCGAAAATCGCCGTTTTATGACATGATCATGAAGGAACCGACGAGTGAAGAAGAGTTTGTAGCGTTCACCGCTCTCTACCGGGTGTCTCCGTACTACCAGTTTGCTCATTTCACGGCAAACCAAGCAATCATTGAGGCATTTGAGAAGGAACAAGAGAAGAACAATCGGGCATTACATGTTATTGATTTTGATGTCTCCTATGGATTTCAATGGCCTTCTCTTATACAGTCTCTGTCCGAGAAGGCAAGCAGTAGCAATCGTATATCGCTCAGAATAACAGGGTTCGGAAGAAGCGTGGATGAGCTACGTGAAACGGAGAACAGACTGACAAGCTTCTCAAAGGGGTTTCGGAATCTTTTTTTTGAATTTCAAGGGTTGTTGAGAAGCTCAAAGCTCATAAACTTGAGGAAGAAGAGAAACGAAACAATTGCAGTGAATTTGGTTTTTCATTTGAATACTTTGAGCAATAATTTGAAGATATCTGACTTGTTAAATTCTGTACATTTACTTAACCCTTCCATTGTAATCTTGGTTGAACAAGAAGGGAGCAAAAGTCCTCGAAGTTTCTTATCGAGATTCATGGAGACTTTGCATTATTTTGCAGCCATGTTTGATTCTTTAGATGATTGTCTGCCATTAGAGAGTGCTGAAAGGTTGAGCATTGAGAAGAACCACCTCGGAAAAGAGATCAAAAGCATGCTCAATTACGACAATAACAACGATGAGAATTGTCCAAAAACTGAAAAGACGGAAACATGGAAGGCGAAGATGGAGAGTCATGGTTTTGAAGGAATAAATCTAAGCTCTAAGTCCGAGATACAAGCAAGACTTCTTTTAAAAATTAGGACTCACTATTCTCCACTTGAGTTTGATGGGAGTAGTGGAGCTGCAGCTGGGTTCAAGGTTTTCGAAAGAGACGACGGAAGGACAATATCTCTTGGGTGGCAAGACAGGTATCTGCTTACAATTTCTGCATGGCACTGCACTATACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

476

Amino Acids

54.24

Weight (kDa)

6.27

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 91 - 470 1.8e-90 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016258)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12280
malus_domestica MD05G1040300.v1.1 MD10G1046300.v1.1
prunus_persica Prupe.8G054000_v2.0.a1
pyrus_communis pycom05g03290
rosa_chinensis RchiOBHm_Chr6g0272211
rosa_laevigata RLG00000013690
rosa_multiflora Rmu_sc0005748.1_g000002
rosa_roxburghii Rroxscaffold_7G00196470
rosa_rugosa Rorug06G0067400
rosa_samantha Rh6BG187000 Rh6CG185100 Rh6DG176800
rosa_wichuraiana Rw0G003840 Rw6G015840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 503
AciI CCGC 3 cut(s) 297, 369, 501
AclWI GGATC 1 cut(s) 265
AcsI RAATTY 3 cut(s) 797, 868, 925
AfaI GTAC 3 cut(s) 350, 524, 933
AflIII ACRYGT 2 cut(s) 276, 607
AgsI TTSAA 9 cut(s) 182, 641, 797, 803, 886, 907, 968, 1220, 1333
AluBI AGCT 8 cut(s) 28, 736, 765, 819, 826, 1236, 1319, 1325
AluI AGCT 8 cut(s) 28, 736, 765, 819, 826, 1236, 1319, 1325
Alw26I GTCTC 5 cut(s) 522, 631, 666, 1010, 1341
AlwI GGATC 1 cut(s) 265
AlwNI CAGNNNCTG 1 cut(s) 664
AoxI GGCC 2 cut(s) 259, 644
ApeKI GCWGC 4 cut(s) 416, 1034, 1319, 1322
ApoI RAATTY 3 cut(s) 797, 868, 925
ArsI GACNNNNNNTTYG 2 cut(s) 300, 332
AspS9I GGNCC 1 cut(s) 259
AsuC2I CCSGG 1 cut(s) 512
AsuHPI GGTGA 3 cut(s) 139, 383, 490
AsuII TTCGAA 1 cut(s) 1341
BanII GRGCYC 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 4 cut(s) 403, 1046, 1306, 1334
BccI CCATC 4 cut(s) 98, 245, 1195, 1298
BceAI ACGGC 3 cut(s) 424, 427, 561
BcgI CGANNNNNNTGC 2 cut(s) 842, 876
BclI TGATCA 1 cut(s) 456
BcnI CCSGG 1 cut(s) 512
BcoDI GTCTC 5 cut(s) 522, 631, 666, 1010, 1341
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 1 cut(s) 1320
BisI GCNGC 4 cut(s) 417, 1035, 1320, 1323
BlpI GCTNAGC 1 cut(s) 322
BlsI GCNGC 4 cut(s) 418, 1036, 1321, 1324
Bme1390I CCNGG 1 cut(s) 512
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 1 cut(s) 512
BmsI GCATC 1 cut(s) 343
BpiI GAAGAC 1 cut(s) 12
Bpu1102I GCTNAGC 1 cut(s) 322
Bpu14I TTCGAA 1 cut(s) 1341
BpuEI CTTGAG 2 cut(s) 856, 1316
BpuMI CCSGG 1 cut(s) 512
BsaAI YACGTR 1 cut(s) 740
BsaJI CCNNGG 2 cut(s) 327, 1108
BsaXI ACNNNNNCTCC 2 cut(s) 970, 1000
Bse1I ACTGG 1 cut(s) 529
BseDI CCNNGG 2 cut(s) 327, 1108
BseGI GGATG 1 cut(s) 736
BseMII CTCAG 3 cut(s) 333, 336, 715
BseNI ACTGG 1 cut(s) 529
BseRI GAGGAG 3 cut(s) 32, 35, 38
BseXI GCAGC 4 cut(s) 403, 1046, 1306, 1334
BseYI CCCAGC 1 cut(s) 1325
BsgI GTGCAG 1 cut(s) 1399
BshFI GGCC 2 cut(s) 261, 646
BsiSI CCGG 1 cut(s) 511
BsmAI GTCTC 5 cut(s) 522, 631, 666, 1010, 1341
BsmBI CGTCTC 1 cut(s) 1341
BsnI GGCC 2 cut(s) 261, 646
Bsp119I TTCGAA 1 cut(s) 1341
Bsp1286I GDGCHC 1 cut(s) 134
Bsp1407I TGTACA 2 cut(s) 348, 931
Bsp143I GATC 4 cut(s) 92, 270, 456, 1119
Bsp1720I GCTNAGC 1 cut(s) 322
BspACI CCGC 3 cut(s) 297, 369, 501
BspANI GGCC 2 cut(s) 261, 646
BspCNI CTCAG 3 cut(s) 334, 335, 714
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 1 cut(s) 468
BspMAI CTGCAG 1 cut(s) 1324
BspPI GGATC 1 cut(s) 265
BspT104I TTCGAA 1 cut(s) 1341
BsrBI CCGCTC 1 cut(s) 503
BsrGI TGTACA 2 cut(s) 348, 931
BsrI ACTGG 1 cut(s) 529
BssECI CCNNGG 2 cut(s) 327, 1108
BssMI GATC 4 cut(s) 92, 270, 456, 1119
BssT1I CCWWGG 1 cut(s) 327
Bst4CI ACNGT 1 cut(s) 660
Bst6I CTCTTC 4 cut(s) 77, 83, 477, 839
BstAUI TGTACA 2 cut(s) 348, 931
BstBAI YACGTR 1 cut(s) 740
BstBI TTCGAA 1 cut(s) 1341
BstC8I GCNNGC 2 cut(s) 679, 1130
BstDEI CTNAG 6 cut(s) 38, 322, 342, 701, 1232, 1239
BstF5I GGATG 1 cut(s) 736
BstKTI GATC 4 cut(s) 95, 273, 459, 1122
BstMAI GTCTC 5 cut(s) 522, 631, 666, 1010, 1341
BstMBI GATC 4 cut(s) 92, 270, 456, 1119
BstMWI GCNNNNNNNGC 3 cut(s) 401, 500, 554
BstNSI RCATGY 3 cut(s) 280, 611, 1132
BstSCI CCNGG 1 cut(s) 510
BstSFI CTRYAG 1 cut(s) 1320
BstV1I GCAGC 4 cut(s) 403, 1046, 1306, 1334
BstV2I GAAGAC 1 cut(s) 12
BsuRI GGCC 2 cut(s) 261, 646
BtsCI GGATG 1 cut(s) 736
BtsI GCAGTG 2 cut(s) 870, 1411
BtsIMutI CAGTG 2 cut(s) 870, 1411
Cac8I GCNNGC 2 cut(s) 679, 1130
CaiI CAGNNNCTG 1 cut(s) 664
CciI TCATGA 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 259
Csp6I GTAC 3 cut(s) 349, 523, 932
CviQI GTAC 3 cut(s) 349, 523, 932
DdeI CTNAG 6 cut(s) 38, 322, 342, 701, 1232, 1239
DpnI GATC 4 cut(s) 94, 272, 458, 1121
DpnII GATC 4 cut(s) 92, 270, 456, 1119
DraI TTTAAA 1 cut(s) 1269
Eam1104I CTCTTC 4 cut(s) 77, 83, 477, 839
EarI CTCTTC 4 cut(s) 77, 83, 477, 839
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 134
Eco32I GATATC 1 cut(s) 912
EcoO109I RGGNCCY 1 cut(s) 259
EcoRV GATATC 1 cut(s) 912
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 327
Esp3I CGTCTC 1 cut(s) 1341
FalI AAGNNNNNCTT 2 cut(s) 1245, 1277
FbaI TGATCA 1 cut(s) 456
Fnu4HI GCNGC 4 cut(s) 417, 1035, 1320, 1323
FokI GGATG 1 cut(s) 743
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 4 cut(s) 417, 1035, 1320, 1323
FspBI CTAG 1 cut(s) 209
GluI GCNGC 4 cut(s) 417, 1035, 1320, 1323
GsaI CCCAGC 1 cut(s) 1329
HaeIII GGCC 2 cut(s) 261, 646
HapII CCGG 1 cut(s) 511
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HindIII AAGCTT 1 cut(s) 763
HinfI GANTC 7 cut(s) 34, 374, 784, 1008, 1046, 1207, 1279
HpaI GTTAAC 1 cut(s) 304
HpaII CCGG 1 cut(s) 511
HphI GGTGA 3 cut(s) 139, 383, 490
Hpy166II GTNNAC 2 cut(s) 304, 498
Hpy188I TCNGA 8 cut(s) 33, 670, 704, 719, 783, 916, 1112, 1246
Hpy188III TCNNGA 3 cut(s) 96, 460, 1005
Hpy8I GTNNAC 2 cut(s) 304, 498
Hpy99I CGWCG 2 cut(s) 475, 1355
HpyCH4III ACNGT 1 cut(s) 660
HpyCH4IV ACGT 1 cut(s) 739
HpyCH4V TGCA 9 cut(s) 47, 404, 419, 863, 1024, 1034, 1322, 1406, 1416
HpyF10VI GCNNNNNNNGC 3 cut(s) 401, 500, 554
HpyF3I CTNAG 6 cut(s) 38, 322, 342, 701, 1232, 1239
HpySE526I ACGT 1 cut(s) 739
Ksp22I TGATCA 1 cut(s) 456
KspAI GTTAAC 1 cut(s) 304
Kzo9I GATC 4 cut(s) 92, 270, 456, 1119
LmnI GCTCC 4 cut(s) 25, 331, 978, 1316
LpnPI CCDG 7 cut(s) 48, 281, 524, 542, 696, 1311, 1369
Lsp1109I GCAGC 4 cut(s) 403, 1046, 1306, 1334
LweI GCATC 1 cut(s) 343
MaeI CTAG 1 cut(s) 209
MaeII ACGT 1 cut(s) 739
MaeIII GTNAC 2 cut(s) 52, 371
MalI GATC 4 cut(s) 94, 272, 458, 1121
MbiI CCGCTC 1 cut(s) 503
MboI GATC 4 cut(s) 92, 270, 456, 1119
MfeI CAATTG 2 cut(s) 48, 858
MhlI GDGCHC 1 cut(s) 134
MlyI GAGTC 3 cut(s) 368, 1216, 1273
MmeI TCCRAC 1 cut(s) 406
MseI TTAA 5 cut(s) 78, 303, 923, 942, 1268
MslI CAYNNNNRTG 1 cut(s) 458
MspA1I CMGCKG 1 cut(s) 1325
MspI CCGG 1 cut(s) 511
MspR9I CCNGG 1 cut(s) 512
MunI CAATTG 2 cut(s) 48, 858
MwoI GCNNNNNNNGC 3 cut(s) 401, 500, 554
NciI CCSGG 1 cut(s) 512
NdeII GATC 4 cut(s) 92, 270, 456, 1119
NlaIV GGNNCC 1 cut(s) 468
NmuCI GTSAC 1 cut(s) 371
NspI RCATGY 3 cut(s) 280, 611, 1132
NspV TTCGAA 1 cut(s) 1341
PaeI GCATGC 1 cut(s) 1132
PagI TCATGA 1 cut(s) 459
PciI ACATGT 2 cut(s) 276, 607
PcsI WCGNNNNNNNCGW 2 cut(s) 723, 1347
PfeI GAWTC 4 cut(s) 34, 784, 1008, 1046
PflFI GACNNNGTC 1 cut(s) 377
PkrI GCNGC 4 cut(s) 418, 1036, 1321, 1324
PleI GAGTC 3 cut(s) 368, 1215, 1273
PpsI GAGTC 3 cut(s) 368, 1215, 1273
Ppu21I YACGTR 1 cut(s) 740
PscI ACATGT 2 cut(s) 276, 607
PspFI CCCAGC 1 cut(s) 1325
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 1 cut(s) 259
PstI CTGCAG 1 cut(s) 1324
PstNI CAGNNNCTG 1 cut(s) 664
PsyI GACNNNGTC 1 cut(s) 377
PvuII CAGCTG 1 cut(s) 1325
RsaI GTAC 3 cut(s) 350, 524, 933
RsaNI GTAC 3 cut(s) 349, 523, 932
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 5 cut(s) 78, 303, 923, 942, 1268
SatI GCNGC 4 cut(s) 417, 1035, 1320, 1323
Sau3AI GATC 4 cut(s) 92, 270, 456, 1119
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 3 cut(s) 368, 1216, 1273
ScrFI CCNGG 1 cut(s) 512
SduI GDGCHC 1 cut(s) 134
SfaNI GCATC 1 cut(s) 343
SfcI CTRYAG 1 cut(s) 1320
SfuI TTCGAA 1 cut(s) 1341
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 2 cut(s) 835, 1295
SmoI CTYRAG 2 cut(s) 835, 1295
SphI GCATGC 1 cut(s) 1132
SsiI CCGC 3 cut(s) 297, 369, 501
SspMI CTAG 1 cut(s) 209
StyD4I CCNGG 1 cut(s) 510
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 660
TaiI ACGT 1 cut(s) 742
TaqI TCGA 4 cut(s) 433, 991, 1004, 1341
TatI WGTACW 2 cut(s) 348, 931
TfiI GAWTC 4 cut(s) 34, 784, 1008, 1046
Tru1I TTAA 5 cut(s) 78, 303, 923, 942, 1268
Tru9I TTAA 5 cut(s) 78, 303, 923, 942, 1268
TscAI CASTG 2 cut(s) 870, 1418
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 4 cut(s) 416, 1034, 1319, 1322
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 5 cut(s) 162, 261, 476, 869, 1000
TspGWI ACGGA 4 cut(s) 510, 761, 1196, 1368
TspRI CASTG 2 cut(s) 870, 1418
Tth111I GACNNNGTC 1 cut(s) 377
XapI RAATTY 3 cut(s) 797, 868, 925
XceI RCATGY 3 cut(s) 280, 611, 1132
XcmI CCANNNNNNNNNTGG 1 cut(s) 958
XspI CTAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.