Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0017537

Physical Location & Seq

Genomic Coordinates Forward (+)

4257999 .. 4260047

Gene Structure 2049 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g03730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2049 bp

ATGAACCAATGTAATCTAATGAAGAGACATATTTTGGGAAGTCAATCCAAGGCCTTGCCGTTTCTGTACAACTACAACAATGCTGCCGCTGCTGTTGGCATTCAAAAACTCTTTTCATTATCAGCACGGTTTGGGACATTGTCTGCAACTCAGGCTTCGTGCGCCAAATTAATTGGGGATGAAGACTCTGAAACTTCAGAAGACGAAGACTGGTTTGCTTCTCGTATCATCCAAGACAAGGACCTTCTCCGCAGCAGCCTCCATATTTCAGGAACAGGGCTTCATGTTCTTGACTTGGTGAATTGTGGCTCTTTGGAAGCGGACCGGACTCTGTACAATAAGCTGCTACACCGGTGCACCCAGACAGCCCAACTTAAACAAGCCAGAATTGTGCATTCACACATCCTTACTTCTCAATTTAAAGATGACCTTCCCATCCGCAACACCATCCTTCACATGTATGTCAAATGTGGCAGTTTGGACGATGCTCACAACCTGTTCGATCAAATGCCTCACAAGGATTTGGTCACTTGGACTGCCTTGATTTCTGGCTATTCTCAACACGATCGACCTCAGGATGCCCTTGTTTTGTTTCCTGAGATGCTCCGCCATGGGCTGGAACCCAACCAGTTCACCTTATCTAGCTTGTTGAAAGCTTCTGGTGCTGTCTCTGATGACAATCATGGCAGGCAGCTTCACACTTATTGCTTTAAGTACGGTCTTGATTCCAATGTCTATGTGGGCACTGCTCTTGTGGACATGTATGCTAGGTGTGGCCATATGGATGAATCCCAGTTGGTCTTTGATGCCTTGGACACCAAGAATGAGGTGTCCTGGAATGCTTTGATTGCTGGGCATGCTAGGAAGGCTCAAGGACAGCATGCACTCGAGTTGTTCTGGAAGATGCTGAGGGAGGGCTTCAAGCCTACACATTTCACTTATTCTAGCGTCTTGACTGCTTGTGCTAGTGCAGGGTCTCTAGAGCAAGGGAAGTGGGTTCACGCCCACATGATCAAATCGGCTGTTACCCTCGTCGCTTTTGTGGGGAATACTCTTCTCGATATGTATGCCAAATCAGGCAGCATTGAGGATGCAAAAAAGGTTTTTGATAGGATGGTTAGACGAGACGTCGTTTCTTGGAATTCAATGCTTACTGGATATGCCCAGCATGGGCTTGGAAAGGAAACTGTGCAACGGTTTGAAGAAATGTTGAAAATTGGAATCCAACCTAATGATATAACCTTTCTTTGTGTTCTTACTGCCTGTAGCCATGCCGGTCTCTTGGATGAAGGACAATATTATTTTGACTTGATGAAACAGTACAACATAGAACCACAGGTTTCACACTATGTGACGATTGTTGATCTTCTTGGTCGTGGAAATCTGCTTGATCGAGCTGAGAAGTTCATAAGAGAAATGCCGATTGAACCTACTGCAGCTGTTTGGGGAGCCTTATTAGGTGCTTGCAGGATGCACAAGAGCATAGAGTTGGGTGCTTATGCTGCGGAACGTAGTTTTGAGCTGGATCCCCATGATTCAGGGCCCCATGTCATACTTTCTAATATCTATGCCTCTGCTGGCAGATGGATTGACGCAGCAAATGTGAGGAAGAAGATGAAAGAGGGTGGGGTGAAAAAGGAACCTGCTTGTAGTTGGGTGGACATCGAGAATGCGGTCCACATGTTTGTAGCGAACGATGATGCTCATCCGCAAAGAGTAGAGATCCTTAGGATGTGGGAGATGATTAGCATGAAGATAAAGGAGATTGGATACGTCCCAGACACTAGCCATGTACTTTTCTATGTGGACCAGCAGGAGAGGGAATTCAAGTTGCAGTACCATAGTGAGAAGCTTGCTCTAGCATTTGCACTTCTTAACACTCCTCCTGGCTCCACCATACGGATTAAGAAGAACATCAGGGTTTGTGGTGATTGTCATTCAGCAATCAAGTTCGTTTCGAAGGTAGAAGGGAGAGAAATCATTGTGAGGGACACCAATCGGTTCCATCATTTCCGTGATGGCTCTTGTTCTTGTGGGGACTACTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

683

Amino Acids

76.78

Weight (kDa)

6.61

Isoelectric Point (pI)

40.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	174 - 219	6.8e-12	PPR repeat family
PPR	PF01535	176 - 206	3.3e-07	PPR repeat
PPR_long	PF17177	274 - 372	3.5e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	275 - 322	2.5e-12	PPR repeat family
PPR_2	PF13041	375 - 423	2.6e-11	PPR repeat family
E_motif	PF20431	492 - 554	1.6e-20	E motif
DYW_deaminase	PF14432	590 - 682	8.1e-42	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017537)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24000
fragaria_vesca	FvH4_3g00130
malus_domestica	MD05G1044700.v1.1
prunus_persica	Prupe.4G001500_v2.0.a1
pyrus_communis	pycom05g03730
rosa_chinensis	RchiOBHm_Chr5g0000171
rosa_laevigata	RLG00000030847
rosa_multiflora	Rmu_ssc0000243.1_g000013
rosa_roxburghii	Rroxscaffold_1G00075920
rosa_rugosa	Rorug04G0382100
rosa_samantha	Rh5CG001500
rosa_wichuraiana	Rw5G000160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1131
Acc36I	ACCTGC	1 cut(s)	1651
AccB7I	CCANNNNNTGG	1 cut(s)	616
AciI	CCGC	8 cut(s)	87, 250, 320, 439, 607, 1505, 1673, 1709
AclWI	GGATC	3 cut(s)	1520, 1533, 1717
AcoI	YGGCCR	1 cut(s)	775
AcsI	RAATTY	2 cut(s)	1141, 1823
AcuI	CTGAAG	1 cut(s)	180
AcyI	GRCGYC	1 cut(s)	1128
AdeI	CACNNNGTG	1 cut(s)	1351
AfaI	GTAC	6 cut(s)	68, 335, 716, 1322, 1794, 1838
AfiI	CCNNNNNNNGG	4 cut(s)	238, 616, 1170, 1899
AflIII	ACRYGT	3 cut(s)	456, 759, 1680
AgeI	ACCGGT	1 cut(s)	351
AgsI	TTSAA	8 cut(s)	104, 652, 922, 1146, 1202, 1213, 1427, 1828
AhdI	GACNNNNNGTC	1 cut(s)	1127
AjnI	CCWGG	2 cut(s)	833, 1885
AluBI	AGCT	8 cut(s)	343, 645, 656, 694, 1397, 1439, 1522, 1852
AluI	AGCT	8 cut(s)	343, 645, 656, 694, 1397, 1439, 1522, 1852
Alw21I	GWGCWC	1 cut(s)	359
Alw26I	GTCTC	5 cut(s)	19, 673, 981, 1119, 1283
Alw44I	GTGCAC	1 cut(s)	355
AlwI	GGATC	3 cut(s)	1520, 1533, 1717
Ama87I	CYCGRG	1 cut(s)	887
AoxI	GGCC	3 cut(s)	51, 775, 1541
ApaI	GGGCCC	1 cut(s)	1545
ApaLI	GTGCAC	1 cut(s)	355
ApoI	RAATTY	2 cut(s)	1141, 1823
AseI	ATTAAT	1 cut(s)	170
AsiGI	ACCGGT	1 cut(s)	351
AspLEI	GCGC	1 cut(s)	164
AspS9I	GGNCC	6 cut(s)	241, 322, 1541, 1542, 1675, 1807
AsuHPI	GGTGA	4 cut(s)	310, 625, 1642, 1940
AsuII	TTCGAA	1 cut(s)	1958
AvaI	CYCGRG	1 cut(s)	887
AvaII	GGWCC	4 cut(s)	241, 322, 1675, 1807
AxyI	CCTNAGG	2 cut(s)	573, 1727
BaeGI	GKGCMC	3 cut(s)	359, 746, 1545
BalI	TGGCCA	1 cut(s)	777
BamHI	GGATCC	1 cut(s)	1525
BanII	GRGCYC	1 cut(s)	1545
BbsI	GAAGAC	3 cut(s)	189, 207, 213
Bbv12I	GWGCWC	1 cut(s)	359
BbvCI	CCTCAGC	1 cut(s)	908
BccI	CCATC	6 cut(s)	443, 455, 1108, 1578, 2012, 2013
BceAI	ACGGC	1 cut(s)	43
BcgI	CGANNNNNNTGC	2 cut(s)	1049, 1083
BciT130I	CCWGG	2 cut(s)	835, 1887
BciVI	GTATCC	1 cut(s)	1763
BclI	TGATCA	1 cut(s)	1011
BcoDI	GTCTC	5 cut(s)	19, 673, 981, 1119, 1283
BfaI	CTAG	8 cut(s)	642, 768, 861, 945, 966, 980, 1785, 1859
BfmI	CTRYAG	2 cut(s)	1264, 1434
BfuAI	ACCTGC	1 cut(s)	1651
BfuI	GTATCC	1 cut(s)	1763
Bme1390I	CCNGG	2 cut(s)	835, 1887
Bme18I	GGWCC	4 cut(s)	241, 322, 1675, 1807
BmeRI	GACNNNNNGTC	1 cut(s)	1127
BmeT110I	CYCGRG	1 cut(s)	887
BmgT120I	GGNCC	6 cut(s)	241, 322, 1541, 1542, 1675, 1807
BmiI	GGNNCC	8 cut(s)	621, 1450, 1527, 1543, 1544, 1641, 1891, 2003
BmrFI	CCNGG	2 cut(s)	835, 1887
BmrI	ACTGGG	1 cut(s)	787
BmsI	GCATC	8 cut(s)	475, 568, 591, 796, 894, 1081, 1461, 1690
BmuI	ACTGGG	1 cut(s)	787
BpiI	GAAGAC	3 cut(s)	189, 207, 213
Bpu10I	CCTNAGC	1 cut(s)	908
Bpu14I	TTCGAA	1 cut(s)	1958
BpuEI	CTTGAG	1 cut(s)	855
BsaBI	GATNNNNATC	2 cut(s)	678, 1704
BsaHI	GRCGYC	1 cut(s)	1128
BsaI	GGTCTC	2 cut(s)	981, 1283
BsaJI	CCNNGG	3 cut(s)	48, 610, 810
BsaWI	WCCGGW	2 cut(s)	324, 351
Bsc4I	CCNNNNNNNGG	4 cut(s)	238, 616, 1170, 1899
Bse118I	RCCGGY	2 cut(s)	351, 1274
Bse1I	ACTGG	5 cut(s)	215, 628, 793, 1159, 2048
Bse21I	CCTNAGG	2 cut(s)	573, 1727
Bse8I	GATNNNNATC	2 cut(s)	678, 1704
BseBI	CCWGG	2 cut(s)	835, 1887
BseDI	CCNNGG	3 cut(s)	48, 610, 810
BseJI	GATNNNNATC	2 cut(s)	678, 1704
BseLI	CCNNNNNNNGG	4 cut(s)	238, 616, 1170, 1899
BseMII	CTCAG	5 cut(s)	164, 587, 588, 899, 1389
BseNI	ACTGG	5 cut(s)	215, 628, 793, 1159, 2048
BseRI	GAGGAG	1 cut(s)	1872
BseSI	GKGCMC	3 cut(s)	359, 746, 1545
BseYI	CCCAGC	2 cut(s)	851, 1164
BsgI	GTGCAG	1 cut(s)	990
Bsh1285I	CGRYCG	1 cut(s)	568
BshFI	GGCC	3 cut(s)	53, 777, 1543
BshTI	ACCGGT	1 cut(s)	351
BsiEI	CGRYCG	1 cut(s)	568
BsiHKAI	GWGCWC	1 cut(s)	359
BsiHKCI	CYCGRG	1 cut(s)	887
BsiSI	CCGG	3 cut(s)	325, 352, 1275
BslFI	GGGAC	3 cut(s)	148, 1760, 2003
BslI	CCNNNNNNNGG	4 cut(s)	238, 616, 1170, 1899
BsmAI	GTCTC	5 cut(s)	19, 673, 981, 1119, 1283
BsmBI	CGTCTC	1 cut(s)	1119
BsmFI	GGGAC	3 cut(s)	148, 1760, 2003
BsmI	GAATGC	4 cut(s)	99, 394, 844, 1675
BsnI	GGCC	3 cut(s)	53, 777, 1543
Bso31I	GGTCTC	2 cut(s)	981, 1283
BsoBI	CYCGRG	1 cut(s)	887
Bsp119I	TTCGAA	1 cut(s)	1958
Bsp120I	GGGCCC	1 cut(s)	1541
Bsp1286I	GDGCHC	3 cut(s)	359, 746, 1545
Bsp1407I	TGTACA	2 cut(s)	66, 333
Bsp143I	GATC	7 cut(s)	502, 565, 1011, 1363, 1390, 1525, 1722
Bsp19I	CCATGG	1 cut(s)	610
BspACI	CCGC	8 cut(s)	87, 250, 320, 439, 607, 1505, 1673, 1709
BspANI	GGCC	3 cut(s)	53, 777, 1543
BspCNI	CTCAG	5 cut(s)	163, 586, 589, 900, 1390
BspLI	GGNNCC	8 cut(s)	621, 1450, 1527, 1543, 1544, 1641, 1891, 2003
BspMAI	CTGCAG	1 cut(s)	1438
BspMI	ACCTGC	1 cut(s)	1651
BspPI	GGATC	3 cut(s)	1520, 1533, 1717
BspT104I	TTCGAA	1 cut(s)	1958
BspTNI	GGTCTC	2 cut(s)	981, 1283
BsrFI	RCCGGY	2 cut(s)	351, 1274
BsrGI	TGTACA	2 cut(s)	66, 333
BsrI	ACTGG	5 cut(s)	215, 628, 793, 1159, 2048
BssAI	RCCGGY	2 cut(s)	351, 1274
BssECI	CCNNGG	3 cut(s)	48, 610, 810
BssMI	GATC	7 cut(s)	502, 565, 1011, 1363, 1390, 1525, 1722
BssNI	GRCGYC	1 cut(s)	1128
BssT1I	CCWWGG	3 cut(s)	48, 610, 810
Bst2UI	CCWGG	2 cut(s)	835, 1887
Bst4CI	ACNGT	5 cut(s)	129, 719, 1189, 1197, 1320
Bst6I	CTCTTC	2 cut(s)	17, 1059
BstACI	GRCGYC	1 cut(s)	1128
BstAUI	TGTACA	2 cut(s)	66, 333
BstBI	TTCGAA	1 cut(s)	1958
BstC8I	GCNNGC	6 cut(s)	689, 858, 882, 1465, 1579, 1854
BstDEI	CTNAG	6 cut(s)	150, 573, 597, 908, 1398, 1727
BstDSI	CCRYGG	1 cut(s)	610
BstHHI	GCGC	1 cut(s)	164
BstKTI	GATC	7 cut(s)	505, 568, 1014, 1366, 1393, 1528, 1725
BstMAI	GTCTC	5 cut(s)	19, 673, 981, 1119, 1283
BstMBI	GATC	7 cut(s)	502, 565, 1011, 1363, 1390, 1525, 1722
BstMCI	CGRYCG	1 cut(s)	568
BstMWI	GCNNNNNNNGC	8 cut(s)	89, 152, 161, 662, 848, 857, 866, 1502
BstNI	CCWGG	2 cut(s)	835, 1887
BstNSI	RCATGY	5 cut(s)	460, 763, 860, 884, 1684
BstSCI	CCNGG	2 cut(s)	833, 1885
BstSFI	CTRYAG	2 cut(s)	1264, 1434
BstSLI	GKGCMC	3 cut(s)	359, 746, 1545
BstV2I	GAAGAC	3 cut(s)	189, 207, 213
BstX2I	RGATCY	2 cut(s)	1525, 1722
BstYI	RGATCY	2 cut(s)	1525, 1722
Bsu36I	CCTNAGG	2 cut(s)	573, 1727
BsuI	GTATCC	1 cut(s)	1763
BsuRI	GGCC	3 cut(s)	53, 777, 1543
BtgI	CCRYGG	1 cut(s)	610
BtsI	GCAGTG	1 cut(s)	744
BtsIMutI	CAGTG	1 cut(s)	744
BveI	ACCTGC	1 cut(s)	1651
Cac8I	GCNNGC	6 cut(s)	689, 858, 882, 1465, 1579, 1854
CfoI	GCGC	1 cut(s)	164
Cfr10I	RCCGGY	2 cut(s)	351, 1274
Cfr13I	GGNCC	6 cut(s)	241, 322, 1541, 1542, 1675, 1807
CpoI	CGGWCCG	1 cut(s)	322
CseI	GACGC	2 cut(s)	937, 1601
Csp6I	GTAC	6 cut(s)	67, 334, 715, 1321, 1793, 1837
CspAI	ACCGGT	1 cut(s)	351
CspCI	CAANNNNNGTGG	2 cut(s)	974, 1009
CspI	CGGWCCG	1 cut(s)	322
CviQI	GTAC	6 cut(s)	67, 334, 715, 1321, 1793, 1837
DdeI	CTNAG	6 cut(s)	150, 573, 597, 908, 1398, 1727
DpnI	GATC	7 cut(s)	504, 567, 1013, 1365, 1392, 1527, 1724
DpnII	GATC	7 cut(s)	502, 565, 1011, 1363, 1390, 1525, 1722
DraI	TTTAAA	1 cut(s)	421
DraIII	CACNNNGTG	1 cut(s)	1351
DriI	GACNNNNNGTC	1 cut(s)	1127
EaeI	YGGCCR	1 cut(s)	775
Eam1104I	CTCTTC	2 cut(s)	17, 1059
Eam1105I	GACNNNNNGTC	1 cut(s)	1127
EarI	CTCTTC	2 cut(s)	17, 1059
EciI	GGCGGA	1 cut(s)	596
Eco130I	CCWWGG	3 cut(s)	48, 610, 810
Eco147I	AGGCCT	1 cut(s)	53
Eco24I	GRGCYC	1 cut(s)	1545
Eco31I	GGTCTC	2 cut(s)	981, 1283
Eco47I	GGWCC	4 cut(s)	241, 322, 1675, 1807
Eco57I	CTGAAG	1 cut(s)	180
Eco81I	CCTNAGG	2 cut(s)	573, 1727
Eco88I	CYCGRG	1 cut(s)	887
EcoO109I	RGGNCCY	3 cut(s)	241, 1541, 1542
EcoRI	GAATTC	2 cut(s)	1141, 1823
EcoRII	CCWGG	2 cut(s)	833, 1885
EcoT14I	CCWWGG	3 cut(s)	48, 610, 810
EcoT38I	GRGCYC	1 cut(s)	1545
ErhI	CCWWGG	3 cut(s)	48, 610, 810
Esp3I	CGTCTC	1 cut(s)	1119
FalI	AAGNNNNNCTT	2 cut(s)	414, 446
FaqI	GGGAC	3 cut(s)	148, 1760, 2003
FauNDI	CATATG	1 cut(s)	780
FbaI	TGATCA	1 cut(s)	1011
FriOI	GRGCYC	1 cut(s)	1545
FspBI	CTAG	8 cut(s)	642, 768, 861, 945, 966, 980, 1785, 1859
GlaI	GCGC	1 cut(s)	163
GsaI	CCCAGC	2 cut(s)	855, 1168
HaeIII	GGCC	3 cut(s)	53, 777, 1543
HapII	CCGG	3 cut(s)	325, 352, 1275
HgaI	GACGC	2 cut(s)	937, 1601
HhaI	GCGC	1 cut(s)	164
Hin1I	GRCGYC	1 cut(s)	1128
Hin6I	GCGC	1 cut(s)	162
HinP1I	GCGC	1 cut(s)	162
HindIII	AAGCTT	2 cut(s)	654, 1850
HinfI	GANTC	6 cut(s)	185, 328, 725, 788, 1221, 1535
HpaII	CCGG	3 cut(s)	325, 352, 1275
HphI	GGTGA	4 cut(s)	310, 625, 1642, 1940
Hpy166II	GTNNAC	7 cut(s)	357, 633, 757, 1000, 1660, 1678, 1807
Hpy188I	TCNGA	3 cut(s)	190, 199, 673
Hpy8I	GTNNAC	7 cut(s)	357, 633, 757, 1000, 1660, 1678, 1807
Hpy99I	CGWCG	2 cut(s)	1037, 1133
HpyAV	CCTTC	7 cut(s)	254, 440, 461, 859, 1283, 1954, 1961
HpyCH4III	ACNGT	5 cut(s)	129, 719, 1189, 1197, 1320
HpyCH4IV	ACGT	3 cut(s)	1128, 1510, 1773
HpyF10VI	GCNNNNNNNGC	8 cut(s)	89, 152, 161, 662, 848, 857, 866, 1502
HpyF3I	CTNAG	6 cut(s)	150, 573, 597, 908, 1398, 1727
HpySE526I	ACGT	3 cut(s)	1128, 1510, 1773
Hsp92I	GRCGYC	1 cut(s)	1128
HspAI	GCGC	1 cut(s)	162
Ksp22I	TGATCA	1 cut(s)	1011
Kzo9I	GATC	7 cut(s)	502, 565, 1011, 1363, 1390, 1525, 1722
LmnI	GCTCC	3 cut(s)	609, 1448, 1895
LweI	GCATC	8 cut(s)	475, 568, 591, 796, 894, 1081, 1461, 1690
MaeI	CTAG	8 cut(s)	642, 768, 861, 945, 966, 980, 1785, 1859
MaeII	ACGT	3 cut(s)	1128, 1510, 1773
MaeIII	GTNAC	3 cut(s)	526, 1024, 1351
MalI	GATC	7 cut(s)	504, 567, 1013, 1365, 1392, 1527, 1724
MboI	GATC	7 cut(s)	502, 565, 1011, 1363, 1390, 1525, 1722
MflI	RGATCY	2 cut(s)	1525, 1722
MhlI	GDGCHC	3 cut(s)	359, 746, 1545
MlsI	TGGCCA	1 cut(s)	777
MluCI	AATT	8 cut(s)	167, 171, 301, 387, 416, 1141, 1215, 1823
MluNI	TGGCCA	1 cut(s)	777
MlyI	GAGTC	2 cut(s)	179, 322
MmeI	TCCRAC	1 cut(s)	1249
Mox20I	TGGCCA	1 cut(s)	777
MscI	TGGCCA	1 cut(s)	777
MseI	TTAA	6 cut(s)	170, 375, 420, 711, 1875, 1905
MslI	CAYNNNNRTG	3 cut(s)	459, 783, 2013
Msp20I	TGGCCA	1 cut(s)	777
MspA1I	CMGCKG	2 cut(s)	89, 1439
MspI	CCGG	3 cut(s)	325, 352, 1275
MspR9I	CCNGG	2 cut(s)	835, 1887
Mva1269I	GAATGC	4 cut(s)	99, 394, 844, 1675
MvaI	CCWGG	2 cut(s)	835, 1887
MwoI	GCNNNNNNNGC	8 cut(s)	89, 152, 161, 662, 848, 857, 866, 1502
NcoI	CCATGG	1 cut(s)	610
NdeI	CATATG	1 cut(s)	780
NdeII	GATC	7 cut(s)	502, 565, 1011, 1363, 1390, 1525, 1722
NlaIV	GGNNCC	8 cut(s)	621, 1450, 1527, 1543, 1544, 1641, 1891, 2003
NmuCI	GTSAC	2 cut(s)	526, 1351
NspI	RCATGY	5 cut(s)	460, 763, 860, 884, 1684
NspV	TTCGAA	1 cut(s)	1958
PaeI	GCATGC	2 cut(s)	860, 884
PaeR7I	CTCGAG	1 cut(s)	887
PceI	AGGCCT	1 cut(s)	53
PciI	ACATGT	3 cut(s)	456, 759, 1680
PctI	GAATGC	4 cut(s)	99, 394, 844, 1675
PfeI	GAWTC	4 cut(s)	725, 788, 1221, 1535
PflFI	GACNNNGTC	1 cut(s)	139
PflMI	CCANNNNNTGG	1 cut(s)	616
PfoI	TCCNGGA	1 cut(s)	833
PinAI	ACCGGT	1 cut(s)	351
Ple19I	CGATCG	1 cut(s)	568
PleI	GAGTC	2 cut(s)	179, 322
PpsI	GAGTC	2 cut(s)	179, 322
PpuMI	RGGWCCY	1 cut(s)	241
PscI	ACATGT	3 cut(s)	456, 759, 1680
PshBI	ATTAAT	1 cut(s)	170
Psp5II	RGGWCCY	1 cut(s)	241
Psp6I	CCWGG	2 cut(s)	833, 1885
PspFI	CCCAGC	2 cut(s)	851, 1164
PspGI	CCWGG	2 cut(s)	833, 1885
PspN4I	GGNNCC	8 cut(s)	621, 1450, 1527, 1543, 1544, 1641, 1891, 2003
PspOMI	GGGCCC	1 cut(s)	1541
PspPI	GGNCC	6 cut(s)	241, 322, 1541, 1542, 1675, 1807
PspPPI	RGGWCCY	1 cut(s)	241
PspXI	VCTCGAGB	1 cut(s)	887
PstI	CTGCAG	1 cut(s)	1438
PsuI	RGATCY	2 cut(s)	1525, 1722
PsyI	GACNNNGTC	1 cut(s)	139
PvuI	CGATCG	1 cut(s)	568
PvuII	CAGCTG	1 cut(s)	1439
RsaI	GTAC	6 cut(s)	68, 335, 716, 1322, 1794, 1838
RsaNI	GTAC	6 cut(s)	67, 334, 715, 1321, 1793, 1837
RseI	CAYNNNNRTG	3 cut(s)	459, 783, 2013
Rsr2I	CGGWCCG	1 cut(s)	322
RsrII	CGGWCCG	1 cut(s)	322
SaqAI	TTAA	6 cut(s)	170, 375, 420, 711, 1875, 1905
Sau3AI	GATC	7 cut(s)	502, 565, 1011, 1363, 1390, 1525, 1722
Sau96I	GGNCC	6 cut(s)	241, 322, 1541, 1542, 1675, 1807
SchI	GAGTC	2 cut(s)	179, 322
ScrFI	CCNGG	2 cut(s)	835, 1887
SduI	GDGCHC	3 cut(s)	359, 746, 1545
SfaNI	GCATC	8 cut(s)	475, 568, 591, 796, 894, 1081, 1461, 1690
SfcI	CTRYAG	2 cut(s)	1264, 1434
Sfr274I	CTCGAG	1 cut(s)	887
SfuI	TTCGAA	1 cut(s)	1958
SgrAI	CRCCGGYG	1 cut(s)	351
SinI	GGWCC	4 cut(s)	241, 322, 1675, 1807
SlaI	CTCGAG	1 cut(s)	887
SmiMI	CAYNNNNRTG	3 cut(s)	459, 783, 2013
SmlI	CTYRAG	2 cut(s)	870, 887
SmoI	CTYRAG	2 cut(s)	870, 887
SphI	GCATGC	2 cut(s)	860, 884
Sse9I	AATT	8 cut(s)	167, 171, 301, 387, 416, 1141, 1215, 1823
SseBI	AGGCCT	1 cut(s)	53
SsiI	CCGC	8 cut(s)	87, 250, 320, 439, 607, 1505, 1673, 1709
SspI	AATATT	1 cut(s)	1298
SspMI	CTAG	8 cut(s)	642, 768, 861, 945, 966, 980, 1785, 1859
StuI	AGGCCT	1 cut(s)	53
StyD4I	CCNGG	2 cut(s)	833, 1885
StyI	CCWWGG	3 cut(s)	48, 610, 810
TaaI	ACNGT	5 cut(s)	129, 719, 1189, 1197, 1320
TaiI	ACGT	3 cut(s)	1131, 1513, 1776
TaqI	TCGA	7 cut(s)	501, 568, 888, 1059, 1393, 1665, 1958
TasI	AATT	8 cut(s)	167, 171, 301, 387, 416, 1141, 1215, 1823
TatI	WGTACW	4 cut(s)	66, 333, 1320, 1792
TauI	GCSGC	1 cut(s)	89
TfiI	GAWTC	4 cut(s)	725, 788, 1221, 1535
Tru1I	TTAA	6 cut(s)	170, 375, 420, 711, 1875, 1905
Tru9I	TTAA	6 cut(s)	170, 375, 420, 711, 1875, 1905
TscAI	CASTG	1 cut(s)	751
TseFI	GTSAC	2 cut(s)	526, 1351
Tsp45I	GTSAC	2 cut(s)	526, 1351
TspGWI	ACGGA	2 cut(s)	1915, 2003
TspRI	CASTG	1 cut(s)	751
Tth111I	GACNNNGTC	1 cut(s)	139
Van91I	CCANNNNNTGG	1 cut(s)	616
VneI	GTGCAC	1 cut(s)	355
VpaK11BI	GGWCC	4 cut(s)	241, 322, 1675, 1807
VspI	ATTAAT	1 cut(s)	170
XapI	RAATTY	2 cut(s)	1141, 1823
XbaI	TCTAGA	1 cut(s)	979
XceI	RCATGY	5 cut(s)	460, 763, 860, 884, 1684
XcmI	CCANNNNNNNNNTGG	2 cut(s)	736, 1172
XhoI	CTCGAG	1 cut(s)	887
XspI	CTAG	8 cut(s)	642, 768, 861, 945, 966, 980, 1785, 1859
ZraI	GACGTC	1 cut(s)	1129

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.