Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0017537

Physical Location & Seq

Genomic Coordinates Reverse (-)

156138 .. 158228

Gene Structure 2091 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG001500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2091 bp

ATGATTCGGAAACAGAGAGAGAGAGGTCGGAAAGGGTCGCCAATCCACATTCGCGGCCATATTCGTTTGTTGGTGAGTTTGATGAAGAGACAGATTTTGTGTAGCCAATCCAAGGCCTTATTATTGTTGTACTTGTACAAGTACAACAATGCTGCTGCTCTTCCTAGTGTTGGTATTCGGAAACTTCCATTATCGGCGCAGATGGGCAGCTCTGCCACTCCGGCTGACTCATACATTTCAGAAGGCGAAGACCATATCATCCAAGACAAGGACCTTCTCCGCCGCACAGATTCGGGAACAACAGGGTCCCTCCATGTTCTGGAGGATTTGGTTAATCGTGCTTCTTTGGAAGCTGACCGGACCCTGTGCAATAAGCTGCTTAACAAATGTACCCAGTCTGGCCAACTTAAACAGGCCAGAATTGTCCATGCTCACATCCTTACTTCCACGTTCAACGATGACCTTCCCATCCACAACACCATTCTCAACACGTACGTCAAGTGCGGCAGCTTGGACGACGCCCACAAGCTGTTCGATGAAATGCCTCACAGGGATTTGGTCACCTGGACAGCCTTGATCTCTGGCTATTCTCAACATGATCGACCTGAGGAAGCACTTGTTCTGTTTCCTCATATGCTTCGCCATGGACTGCAACCCAACCAGTTCACGCTGTCTAGCTTGCTCAAGGCTGCTGGTGCTGGGCCTGACCCTGATGATAAGCACGGCAGGCAGCTTCATGCCCATTGCTTCAAGTGCGGTTTTGATTCCAATGTTTATGTGGGCACTTCTCTCGTGGACATGTATGCTAGGTGTGGCCGTATGGACGAATCCCAGATCATCTTTGACGCTCTCGACACCAAGAATGAGGTGTCTTGGAATGCTTTGATCGCCGGGCATGCTAGGAAGGGTCAAGGAGAGCATGCTCTGAGGTTGTTCTGGAAGATGCTGAGGGGAGGATTCAACCCCACTCATTTCACTTATTCTAGTGTGTTTACTGTTTGTGCCAGTGCAGGGTCTATGGAGCAAGGTAAGTGGGTTCACTCCCATATGATCAAATCGGGTGTTAACCTTGTTGCTTTTGTTGGGAATACTCTTCTCGACATGTATGCCAAGTCAGGCAGCATTGATGATGCTAAAAAGGTATTTGACCGGTTGGTTAGACGAGACATTGTTTCTTTTAATTCGATGCTTACTGGATACTCCCAGCATGGGCTTGGGAAGGAAACTGTGCAGCTGTTTGCACAAATGCTTAGGATTGGAATTCAACCTAATGATATAACATTCCTCTGTCTTCTTACTGCCTGTAGCCATGCTCGCCTCTTGGATGAAGGACAGTATTATTTTCACTTGATGAGGAAAAGGTTCAAAATAGAACCACAGGTTTGGCATTACGTTACAATGGTTGATCTTCTTGGCCGAGCAGGTCTTCTTGATAGAGCTGACAAGTTCATAAGAGAAATGCCGATTGAACCCACTGCAGCTGTCTGGGGAGCCTTGCTAGGCGCTTGTAGGATGCACAACAACATCGACTTGGGTGCCTATGCTGCTGAGCGTGCTTTTGAGCTTGATCCTTATGATTCAGGACCCTATATTATACTCGCTAATATGTACGCATCTGCTGGAAGATTGAATGATGCTGCAAAAGTGAGAAAGATGATGAAAGAGAGTGGGGTGAAAAAGGAACCTGCCTGTAGTTGGGTGGAGATTGAGAATGCAGTCCACATGTTTGTGGCAAACGATGATGCACACCCGCAAAGAGCAGAGATATATAGGATGTGGGAGATGATTAGCAAGAAGATAAAGGAGGTCGGATACGTTCCAGACACTAGCCATGTACTCTTCTTTGTGGACCAGCAGGAGAGGGAAGTCAAGTTGCAGTTTCACAGTGAGAAGCTTGCACTCGCATTTGCGCTTCTTAACACTTCTCCTGGATCCACCATCCGGATCAAGAAGAACATTAGGGTTTGTGGTGATTGTCATTCGGCAATCAAGTATGTCTCAAAGATGGAAAGGCGAGAAATCATTGTGAGAGACACCAATCGGTTCCATCATTTCCGTGATGGCTCTTGTTCTTGTGGGGACTATTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

696

Amino Acids

78.6

Weight (kDa)

8.86

Isoelectric Point (pI)

39.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	185 - 230	1.3e-11	PPR repeat family
PPR	PF01535	187 - 217	2.7e-07	PPR repeat
PPR_2	PF13041	288 - 335	1.5e-12	PPR repeat family
PPR	PF01535	290 - 320	1.3e-06	PPR repeat
PPR_2	PF13041	388 - 436	2.7e-11	PPR repeat family
E_motif	PF20431	506 - 568	8.3e-21	E motif
DYW_deaminase	PF14432	604 - 696	2.2e-41	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0017537)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24000
fragaria_vesca	FvH4_3g00130
malus_domestica	MD05G1044700.v1.1
prunus_persica	Prupe.4G001500_v2.0.a1
pyrus_communis	pycom05g03730
rosa_chinensis	RchiOBHm_Chr5g0000171
rosa_laevigata	RLG00000030847
rosa_multiflora	Rmu_ssc0000243.1_g000013
rosa_roxburghii	Rroxscaffold_1G00075920
rosa_rugosa	Rorug04G0382100
rosa_samantha	Rh5CG001500
rosa_wichuraiana	Rw5G000160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1412, 1693
AccB1I	GGYRCC	1 cut(s)	1535
AccB7I	CCANNNNNTGG	1 cut(s)	319
AccII	CGCG	1 cut(s)	54
AccIII	TCCGGA	1 cut(s)	1941
AciI	CCGC	6 cut(s)	54, 280, 283, 504, 756, 1751
AclWI	GGATC	4 cut(s)	1562, 1926, 1939, 1952
AcoI	YGGCCR	4 cut(s)	55, 400, 814, 1414
AcsI	RAATTY	1 cut(s)	1260
AcyI	GRCGYC	1 cut(s)	519
AfaI	GTAC	7 cut(s)	131, 137, 143, 391, 494, 1610, 1836
AfiI	CCNNNNNNNGG	8 cut(s)	112, 170, 268, 319, 1011, 1209, 1695, 1941
AflIII	ACRYGT	4 cut(s)	489, 798, 1101, 1722
AgeI	ACCGGT	1 cut(s)	1149
AgsI	TTSAA	7 cut(s)	454, 751, 961, 1265, 1366, 1469, 1630
AjnI	CCWGG	2 cut(s)	563, 1927
Alw26I	GTCTC	4 cut(s)	82, 1158, 2002, 2025
AlwI	GGATC	4 cut(s)	1562, 1926, 1939, 1952
Aor13HI	TCCGGA	1 cut(s)	1941
AoxI	GGCC	7 cut(s)	55, 114, 400, 414, 701, 814, 1414
ApoI	RAATTY	1 cut(s)	1260
AsiGI	ACCGGT	1 cut(s)	1149
Asp700I	GAANNNNTTC	1 cut(s)	1361
AspLEI	GCGC	3 cut(s)	199, 1505, 1912
AspS9I	GGNCC	6 cut(s)	271, 306, 360, 701, 1583, 1849
AsuC2I	CCSGG	1 cut(s)	892
AsuHPI	GGTGA	4 cut(s)	85, 553, 1684, 1982
AvaII	GGWCC	5 cut(s)	271, 306, 360, 1583, 1849
AxyI	CCTNAGG	1 cut(s)	606
BaeGI	GKGCMC	1 cut(s)	785
BaeI	ACNNNNGTAYC	2 cut(s)	373, 406
BalI	TGGCCA	1 cut(s)	402
BamHI	GGATCC	1 cut(s)	1931
BanI	GGYRCC	1 cut(s)	1535
BauI	CACGAG	1 cut(s)	791
BbsI	GAAGAC	3 cut(s)	255, 1283, 1418
BbvCI	CCTCAGC	1 cut(s)	947
BccI	CCATC	6 cut(s)	196, 476, 1946, 1999, 2054, 2055
BceAI	ACGGC	2 cut(s)	739, 801
BcgI	CGANNNNNNTGC	6 cut(s)	772, 806, 1088, 1122, 1517, 1551
BciT130I	CCWGG	2 cut(s)	565, 1929
BciVI	GTATCC	2 cut(s)	1190, 1805
BclI	TGATCA	1 cut(s)	1050
BcnI	CCSGG	1 cut(s)	892
BcoDI	GTCTC	4 cut(s)	82, 1158, 2002, 2025
BfaI	CTAG	7 cut(s)	165, 675, 807, 900, 984, 1499, 1827
BfmI	CTRYAG	3 cut(s)	1303, 1476, 1690
BfoI	RGCGCY	1 cut(s)	1506
BfuAI	ACCTGC	2 cut(s)	1412, 1693
BfuI	GTATCC	2 cut(s)	1190, 1805
BglI	GCCNNNNNGGC	1 cut(s)	221
BlpI	GCTNAGC	1 cut(s)	1548
Bme1390I	CCNGG	3 cut(s)	565, 892, 1929
Bme18I	GGWCC	5 cut(s)	271, 306, 360, 1583, 1849
BmgT120I	GGNCC	6 cut(s)	271, 306, 360, 701, 1583, 1849
BmiI	GGNNCC	9 cut(s)	307, 308, 362, 1492, 1537, 1585, 1683, 1933, 2045
BmrFI	CCNGG	3 cut(s)	565, 892, 1929
BmrI	ACTGGG	1 cut(s)	388
BmsI	GCATC	7 cut(s)	933, 1120, 1176, 1503, 1622, 1624, 1732
BmuI	ACTGGG	1 cut(s)	388
BpiI	GAAGAC	3 cut(s)	255, 1283, 1418
BpmI	CTGGAG	1 cut(s)	341
Bpu10I	CCTNAGC	2 cut(s)	947, 1250
Bpu1102I	GCTNAGC	1 cut(s)	1548
BpuEI	CTTGAG	1 cut(s)	668
BpuMI	CCSGG	1 cut(s)	892
BsaAI	YACGTR	1 cut(s)	492
BsaHI	GRCGYC	1 cut(s)	519
BsaJI	CCNNGG	2 cut(s)	111, 643
BsaWI	WCCGGW	3 cut(s)	357, 1149, 1941
Bsc4I	CCNNNNNNNGG	8 cut(s)	112, 170, 268, 319, 1011, 1209, 1695, 1941
Bse118I	RCCGGY	1 cut(s)	1149
Bse1I	ACTGG	4 cut(s)	394, 661, 1005, 1198
Bse21I	CCTNAGG	1 cut(s)	606
Bse3DI	GCAATG	1 cut(s)	742
BseAI	TCCGGA	1 cut(s)	1941
BseBI	CCWGG	2 cut(s)	565, 1929
BseDI	CCNNGG	2 cut(s)	111, 643
BseGI	GGATG	7 cut(s)	258, 435, 468, 1330, 1518, 1779, 1938
BseLI	CCNNNNNNNGG	8 cut(s)	112, 170, 268, 319, 1011, 1209, 1695, 1941
BseMI	GCAATG	1 cut(s)	742
BseMII	CTCAG	4 cut(s)	597, 917, 938, 1539
BseNI	ACTGG	4 cut(s)	394, 661, 1005, 1198
BseSI	GKGCMC	1 cut(s)	785
BseYI	CCCAGC	2 cut(s)	698, 1203
BsgI	GTGCAG	2 cut(s)	1029, 1250
Bsh1236I	CGCG	1 cut(s)	54
BshFI	GGCC	7 cut(s)	57, 116, 402, 416, 703, 816, 1416
BshNI	GGYRCC	1 cut(s)	1535
BshTI	ACCGGT	1 cut(s)	1149
BsiSI	CCGG	5 cut(s)	221, 358, 891, 1150, 1942
BsiWI	CGTACG	1 cut(s)	492
BslFI	GGGAC	1 cut(s)	292
BslI	CCNNNNNNNGG	8 cut(s)	112, 170, 268, 319, 1011, 1209, 1695, 1941
BsmAI	GTCTC	4 cut(s)	82, 1158, 2002, 2025
BsmFI	GGGAC	1 cut(s)	292
BsmI	GAATGC	2 cut(s)	883, 1717
BsnI	GGCC	7 cut(s)	57, 116, 402, 416, 703, 816, 1416
Bsp1286I	GDGCHC	1 cut(s)	785
Bsp13I	TCCGGA	1 cut(s)	1941
Bsp1407I	TGTACA	1 cut(s)	135
Bsp143I	GATC	9 cut(s)	576, 598, 834, 885, 1050, 1405, 1567, 1931, 1944
Bsp1720I	GCTNAGC	1 cut(s)	1548
Bsp19I	CCATGG	1 cut(s)	643
BspACI	CCGC	6 cut(s)	54, 280, 283, 504, 756, 1751
BspANI	GGCC	7 cut(s)	57, 116, 402, 416, 703, 816, 1416
BspCNI	CTCAG	4 cut(s)	598, 918, 939, 1540
BspEI	TCCGGA	1 cut(s)	1941
BspFNI	CGCG	1 cut(s)	54
BspLI	GGNNCC	9 cut(s)	307, 308, 362, 1492, 1537, 1585, 1683, 1933, 2045
BspMAI	CTGCAG	1 cut(s)	1480
BspMI	ACCTGC	2 cut(s)	1412, 1693
BspPI	GGATC	4 cut(s)	1562, 1926, 1939, 1952
BspQI	GCTCTTC	1 cut(s)	165
BspT107I	GGYRCC	1 cut(s)	1535
BsrDI	GCAATG	1 cut(s)	742
BsrFI	RCCGGY	1 cut(s)	1149
BsrGI	TGTACA	1 cut(s)	135
BsrI	ACTGG	4 cut(s)	394, 661, 1005, 1198
BssAI	RCCGGY	1 cut(s)	1149
BssECI	CCNNGG	2 cut(s)	111, 643
BssMI	GATC	9 cut(s)	576, 598, 834, 885, 1050, 1405, 1567, 1931, 1944
BssNI	GRCGYC	1 cut(s)	519
BssSI	CACGAG	1 cut(s)	791
BssT1I	CCWWGG	2 cut(s)	111, 643
Bst2BI	CACGAG	1 cut(s)	791
Bst2UI	CCWGG	2 cut(s)	565, 1929
Bst4CI	ACNGT	4 cut(s)	997, 1228, 1335, 1886
Bst6I	CTCTTC	4 cut(s)	80, 165, 1098, 1844
BstACI	GRCGYC	1 cut(s)	519
BstAUI	TGTACA	1 cut(s)	135
BstBAI	YACGTR	1 cut(s)	492
BstC8I	GCNNGC	7 cut(s)	680, 728, 897, 921, 1315, 1554, 1896
BstDEI	CTNAG	5 cut(s)	606, 926, 947, 1250, 1548
BstDSI	CCRYGG	1 cut(s)	643
BstEII	GGTNACC	1 cut(s)	559
BstF5I	GGATG	7 cut(s)	258, 435, 468, 1330, 1518, 1779, 1938
BstFNI	CGCG	1 cut(s)	54
BstH2I	RGCGCY	1 cut(s)	1506
BstHHI	GCGC	3 cut(s)	199, 1505, 1912
BstKTI	GATC	9 cut(s)	579, 601, 837, 888, 1053, 1408, 1570, 1934, 1947
BstMAI	GTCTC	4 cut(s)	82, 1158, 2002, 2025
BstMBI	GATC	9 cut(s)	576, 598, 834, 885, 1050, 1405, 1567, 1931, 1944
BstMWI	GCNNNNNNNGC	9 cut(s)	221, 695, 727, 753, 887, 896, 1314, 1544, 1553
BstNI	CCWGG	2 cut(s)	565, 1929
BstNSI	RCATGY	5 cut(s)	802, 899, 923, 1105, 1726
BstPI	GGTNACC	1 cut(s)	559
BstSCI	CCNGG	3 cut(s)	563, 890, 1927
BstSFI	CTRYAG	3 cut(s)	1303, 1476, 1690
BstSLI	GKGCMC	1 cut(s)	785
BstUI	CGCG	1 cut(s)	54
BstV2I	GAAGAC	3 cut(s)	255, 1283, 1418
BstX2I	RGATCY	1 cut(s)	1931
BstXI	CCANNNNNNTGG	1 cut(s)	1383
BstYI	RGATCY	1 cut(s)	1931
Bsu36I	CCTNAGG	1 cut(s)	606
BsuI	GTATCC	2 cut(s)	1190, 1805
BsuRI	GGCC	7 cut(s)	57, 116, 402, 416, 703, 816, 1416
BtgI	CCRYGG	1 cut(s)	643
BtsCI	GGATG	7 cut(s)	258, 435, 468, 1330, 1518, 1779, 1938
BtsI	GCAGTG	1 cut(s)	1473
BtsIMutI	CAGTG	3 cut(s)	1012, 1473, 1891
BveI	ACCTGC	2 cut(s)	1412, 1693
Cac8I	GCNNGC	7 cut(s)	680, 728, 897, 921, 1315, 1554, 1896
CfoI	GCGC	3 cut(s)	199, 1505, 1912
Cfr10I	RCCGGY	1 cut(s)	1149
Cfr13I	GGNCC	6 cut(s)	271, 306, 360, 701, 1583, 1849
CseI	GACGC	2 cut(s)	527, 854
Csp6I	GTAC	7 cut(s)	130, 136, 142, 390, 493, 1609, 1835
CspAI	ACCGGT	1 cut(s)	1149
CspCI	CAANNNNNGTGG	2 cut(s)	1013, 1048
CviQI	GTAC	7 cut(s)	130, 136, 142, 390, 493, 1609, 1835
DdeI	CTNAG	5 cut(s)	606, 926, 947, 1250, 1548
DpnI	GATC	9 cut(s)	578, 600, 836, 887, 1052, 1407, 1569, 1933, 1946
DpnII	GATC	9 cut(s)	576, 598, 834, 885, 1050, 1405, 1567, 1931, 1944
EaeI	YGGCCR	4 cut(s)	55, 400, 814, 1414
Eam1104I	CTCTTC	4 cut(s)	80, 165, 1098, 1844
EarI	CTCTTC	4 cut(s)	80, 165, 1098, 1844
EciI	GGCGGA	1 cut(s)	269
Eco130I	CCWWGG	2 cut(s)	111, 643
Eco147I	AGGCCT	1 cut(s)	116
Eco47I	GGWCC	5 cut(s)	271, 306, 360, 1583, 1849
Eco81I	CCTNAGG	1 cut(s)	606
Eco91I	GGTNACC	1 cut(s)	559
EcoO109I	RGGNCCY	3 cut(s)	271, 306, 1583
EcoO65I	GGTNACC	1 cut(s)	559
EcoRI	GAATTC	1 cut(s)	1260
EcoRII	CCWGG	2 cut(s)	563, 1927
EcoT14I	CCWWGG	2 cut(s)	111, 643
ErhI	CCWWGG	2 cut(s)	111, 643
FaqI	GGGAC	1 cut(s)	292
FauI	CCCGC	1 cut(s)	1758
FauNDI	CATATG	2 cut(s)	633, 1047
FbaI	TGATCA	1 cut(s)	1050
FokI	GGATG	7 cut(s)	245, 422, 455, 1337, 1525, 1786, 1925
FspBI	CTAG	7 cut(s)	165, 675, 807, 900, 984, 1499, 1827
GlaI	GCGC	3 cut(s)	198, 1504, 1911
GsaI	CCCAGC	2 cut(s)	702, 1207
GsuI	CTGGAG	1 cut(s)	341
HaeII	RGCGCY	1 cut(s)	1506
HaeIII	GGCC	7 cut(s)	57, 116, 402, 416, 703, 816, 1416
HapII	CCGG	5 cut(s)	221, 358, 891, 1150, 1942
HgaI	GACGC	2 cut(s)	527, 854
HhaI	GCGC	3 cut(s)	199, 1505, 1912
Hin1I	GRCGYC	1 cut(s)	519
Hin6I	GCGC	3 cut(s)	197, 1503, 1910
HinP1I	GCGC	3 cut(s)	197, 1503, 1910
HincII	GTYRAC	1 cut(s)	1066
HindII	GTYRAC	1 cut(s)	1066
HindIII	AAGCTT	1 cut(s)	1892
HinfI	GANTC	7 cut(s)	4, 227, 290, 764, 827, 957, 1577
HpaI	GTTAAC	1 cut(s)	1066
HpaII	CCGG	5 cut(s)	221, 358, 891, 1150, 1942
HphI	GGTGA	4 cut(s)	85, 553, 1684, 1982
Hpy166II	GTNNAC	7 cut(s)	666, 796, 993, 1039, 1066, 1720, 1849
Hpy188I	TCNGA	6 cut(s)	9, 30, 180, 241, 927, 1811
Hpy8I	GTNNAC	7 cut(s)	666, 796, 993, 1039, 1066, 1720, 1849
Hpy99I	CGWCG	1 cut(s)	521
HpyAV	CCTTC	6 cut(s)	236, 284, 473, 898, 1213, 1322
HpyCH4III	ACNGT	4 cut(s)	997, 1228, 1335, 1886
HpyCH4IV	ACGT	5 cut(s)	449, 491, 495, 1392, 1815
HpyF10VI	GCNNNNNNNGC	9 cut(s)	221, 695, 727, 753, 887, 896, 1314, 1544, 1553
HpyF3I	CTNAG	5 cut(s)	606, 926, 947, 1250, 1548
HpySE526I	ACGT	5 cut(s)	449, 491, 495, 1392, 1815
Hsp92I	GRCGYC	1 cut(s)	519
HspAI	GCGC	3 cut(s)	197, 1503, 1910
KflI	GGGWCCC	1 cut(s)	306
Kpn2I	TCCGGA	1 cut(s)	1941
Ksp22I	TGATCA	1 cut(s)	1050
KspAI	GTTAAC	1 cut(s)	1066
Kzo9I	GATC	9 cut(s)	576, 598, 834, 885, 1050, 1405, 1567, 1931, 1944
LguI	GCTCTTC	1 cut(s)	165
LmnI	GCTCC	2 cut(s)	1021, 1490
LweI	GCATC	7 cut(s)	933, 1120, 1176, 1503, 1622, 1624, 1732
MaeI	CTAG	7 cut(s)	165, 675, 807, 900, 984, 1499, 1827
MaeII	ACGT	5 cut(s)	449, 491, 495, 1392, 1815
MaeIII	GTNAC	2 cut(s)	559, 1393
MalI	GATC	9 cut(s)	578, 600, 836, 887, 1052, 1407, 1569, 1933, 1946
MboI	GATC	9 cut(s)	576, 598, 834, 885, 1050, 1405, 1567, 1931, 1944
MflI	RGATCY	1 cut(s)	1931
MhlI	GDGCHC	1 cut(s)	785
MlsI	TGGCCA	1 cut(s)	402
MluCI	AATT	3 cut(s)	420, 1180, 1260
MluNI	TGGCCA	1 cut(s)	402
MlyI	GAGTC	1 cut(s)	221
MmeI	TCCRAC	2 cut(s)	8, 1789
Mox20I	TGGCCA	1 cut(s)	402
MroI	TCCGGA	1 cut(s)	1941
MroXI	GAANNNNTTC	1 cut(s)	1361
MscI	TGGCCA	1 cut(s)	402
MseI	TTAA	6 cut(s)	333, 381, 408, 1065, 1179, 1917
MslI	CAYNNNNRTG	2 cut(s)	1727, 2055
Msp20I	TGGCCA	1 cut(s)	402
MspA1I	CMGCKG	2 cut(s)	1234, 1481
MspI	CCGG	5 cut(s)	221, 358, 891, 1150, 1942
MspR9I	CCNGG	3 cut(s)	565, 892, 1929
Mva1269I	GAATGC	2 cut(s)	883, 1717
MvaI	CCWGG	2 cut(s)	565, 1929
MvnI	CGCG	1 cut(s)	54
MwoI	GCNNNNNNNGC	9 cut(s)	221, 695, 727, 753, 887, 896, 1314, 1544, 1553
NciI	CCSGG	1 cut(s)	892
NcoI	CCATGG	1 cut(s)	643
NdeI	CATATG	2 cut(s)	633, 1047
NdeII	GATC	9 cut(s)	576, 598, 834, 885, 1050, 1405, 1567, 1931, 1944
NlaIV	GGNNCC	9 cut(s)	307, 308, 362, 1492, 1537, 1585, 1683, 1933, 2045
NmeAIII	GCCGAG	1 cut(s)	1442
NmuCI	GTSAC	1 cut(s)	559
NspI	RCATGY	5 cut(s)	802, 899, 923, 1105, 1726
PaeI	GCATGC	2 cut(s)	899, 923
PceI	AGGCCT	1 cut(s)	116
PciI	ACATGT	3 cut(s)	798, 1101, 1722
PciSI	GCTCTTC	1 cut(s)	165
PctI	GAATGC	2 cut(s)	883, 1717
PdmI	GAANNNNTTC	1 cut(s)	1361
PfeI	GAWTC	6 cut(s)	4, 290, 764, 827, 957, 1577
Pfl23II	CGTACG	1 cut(s)	492
PflMI	CCANNNNNTGG	1 cut(s)	319
PfoI	TCCNGGA	1 cut(s)	1927
PinAI	ACCGGT	1 cut(s)	1149
PleI	GAGTC	1 cut(s)	221
PpsI	GAGTC	1 cut(s)	221
Ppu21I	YACGTR	1 cut(s)	492
PpuMI	RGGWCCY	3 cut(s)	271, 306, 1583
PscI	ACATGT	3 cut(s)	798, 1101, 1722
Psp5II	RGGWCCY	3 cut(s)	271, 306, 1583
Psp6I	CCWGG	2 cut(s)	563, 1927
PspEI	GGTNACC	1 cut(s)	559
PspFI	CCCAGC	2 cut(s)	698, 1203
PspGI	CCWGG	2 cut(s)	563, 1927
PspLI	CGTACG	1 cut(s)	492
PspN4I	GGNNCC	9 cut(s)	307, 308, 362, 1492, 1537, 1585, 1683, 1933, 2045
PspPI	GGNCC	6 cut(s)	271, 306, 360, 701, 1583, 1849
PspPPI	RGGWCCY	3 cut(s)	271, 306, 1583
PstI	CTGCAG	1 cut(s)	1480
PsuI	RGATCY	1 cut(s)	1931
PvuII	CAGCTG	2 cut(s)	1234, 1481
RsaI	GTAC	7 cut(s)	131, 137, 143, 391, 494, 1610, 1836
RsaNI	GTAC	7 cut(s)	130, 136, 142, 390, 493, 1609, 1835
RseI	CAYNNNNRTG	2 cut(s)	1727, 2055
SapI	GCTCTTC	1 cut(s)	165
SaqAI	TTAA	6 cut(s)	333, 381, 408, 1065, 1179, 1917
Sau3AI	GATC	9 cut(s)	576, 598, 834, 885, 1050, 1405, 1567, 1931, 1944
Sau96I	GGNCC	6 cut(s)	271, 306, 360, 701, 1583, 1849
SchI	GAGTC	1 cut(s)	221
ScrFI	CCNGG	3 cut(s)	565, 892, 1929
SduI	GDGCHC	1 cut(s)	785
SfaNI	GCATC	7 cut(s)	933, 1120, 1176, 1503, 1622, 1624, 1732
SfcI	CTRYAG	3 cut(s)	1303, 1476, 1690
SinI	GGWCC	5 cut(s)	271, 306, 360, 1583, 1849
SmiMI	CAYNNNNRTG	2 cut(s)	1727, 2055
SmlI	CTYRAG	1 cut(s)	683
SmoI	CTYRAG	1 cut(s)	683
SphI	GCATGC	2 cut(s)	899, 923
Sse9I	AATT	3 cut(s)	420, 1180, 1260
SseBI	AGGCCT	1 cut(s)	116
SsiI	CCGC	6 cut(s)	54, 280, 283, 504, 756, 1751
SspMI	CTAG	7 cut(s)	165, 675, 807, 900, 984, 1499, 1827
StuI	AGGCCT	1 cut(s)	116
StyD4I	CCNGG	3 cut(s)	563, 890, 1927
StyI	CCWWGG	2 cut(s)	111, 643
TaaI	ACNGT	4 cut(s)	997, 1228, 1335, 1886
TaiI	ACGT	5 cut(s)	452, 494, 498, 1395, 1818
TaqI	TCGA	6 cut(s)	534, 601, 852, 1098, 1184, 1527
TasI	AATT	3 cut(s)	420, 1180, 1260
TatI	WGTACW	4 cut(s)	129, 135, 141, 1834
TauI	GCSGC	3 cut(s)	57, 285, 507
TfiI	GAWTC	6 cut(s)	4, 290, 764, 827, 957, 1577
Tru1I	TTAA	6 cut(s)	333, 381, 408, 1065, 1179, 1917
Tru9I	TTAA	6 cut(s)	333, 381, 408, 1065, 1179, 1917
TscAI	CASTG	3 cut(s)	1012, 1480, 1891
TseFI	GTSAC	1 cut(s)	559
Tsp45I	GTSAC	1 cut(s)	559
TspDTI	ATGAA	6 cut(s)	98, 552, 725, 1341, 1438, 1673
TspGWI	ACGGA	1 cut(s)	2045
TspRI	CASTG	3 cut(s)	1012, 1480, 1891
Van91I	CCANNNNNTGG	1 cut(s)	319
VpaK11BI	GGWCC	5 cut(s)	271, 306, 360, 1583, 1849
XapI	RAATTY	1 cut(s)	1260
XceI	RCATGY	5 cut(s)	802, 899, 923, 1105, 1726
XcmI	CCANNNNNNNNNTGG	2 cut(s)	775, 1211
XmnI	GAANNNNTTC	1 cut(s)	1361
XspI	CTAG	7 cut(s)	165, 675, 807, 900, 984, 1499, 1827

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.