pycom05g08810

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009594
Physical Location & Seq
Reverse (-)
11593460 .. 11594881
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 624 bp
ATGGGAGAATCTATGCCTCCTCGTCGCATTTCCGTTCATCAAGCTCTCGGAGGTGGCCATGTTGCCGATGTGCTACTGTGGAAGAAATGGAGTGGAGGAGTTTTACTGCTGAGCTCTACGACGGCGTCTTGGTTGCTGTTTGAGCGAGCTGGCTACAATCTCTTGTCCTTTGTGGCTAATGTTCTACTGCTACTTGTAGTTATCCTCTTCCTCTGGGCCAAATCGGCTTCTCTTCTCAACAGACCTTTGCCTCCTCTCCCTGATATGGAAATCTCAGAGGCCAATGTAGTGAAGGCTGCTGATGCACTGCATGCTGGGATTAATCGTGGACTGTTTATTGCACGAGACATTGCTATTGGGAGAAATTGGAAACTCTTTCTTCAGGTCGCTTTCTGCTTGTGGCTAGCTTCTTACATTGGAAGTTTCTTCAACTTCCGTACTCTTGTCTACATTGGGGTTCTCCTAAGTCTTTCAGTGCCTGTGTTGTATGATAAGTATCAGGACCACATTGATGATAAGCTGCATGTAGCAAACAGGGTAATACAAGCACAGTACAGGAAAATTGATGATAGCATCCTAAAGAAAATTCCTTTGCAATCAAACAAGGAGAAGAAGCTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

208

Amino Acids

23.24

Weight (kDa)

9.68

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 21 - 175 6.9e-53 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 447
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 1 cut(s) 585
AcuI CTGAAG 1 cut(s) 365
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 2 cut(s) 439, 554
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 1 cut(s) 430
AluBI AGCT 6 cut(s) 44, 114, 149, 407, 520, 616
AluI AGCT 6 cut(s) 44, 114, 149, 407, 520, 616
Alw21I GWGCWC 1 cut(s) 116
Alw26I GTCTC 1 cut(s) 339
AlwNI CAGNNNCTG 1 cut(s) 479
AoxI GGCC 3 cut(s) 55, 216, 279
ApeKI GCWGC 2 cut(s) 296, 520
ApoI RAATTY 1 cut(s) 585
AseI ATTAAT 1 cut(s) 321
AspS9I GGNCC 2 cut(s) 216, 502
AsuNHI GCTAGC 1 cut(s) 403
AvaII GGWCC 1 cut(s) 502
BalI TGGCCA 1 cut(s) 57
BanII GRGCYC 1 cut(s) 116
BauI CACGAG 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 116
BbvI GCAGC 2 cut(s) 283, 507
BceAI ACGGC 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 339
BfaI CTAG 1 cut(s) 404
BfmI CTRYAG 1 cut(s) 617
BglI GCCNNNNNGGC 1 cut(s) 224
BisI GCNGC 2 cut(s) 297, 521
BlpI GCTNAGC 1 cut(s) 110
BlsI GCNGC 2 cut(s) 298, 522
Bme18I GGWCC 1 cut(s) 502
BmgT120I GGNCC 2 cut(s) 216, 502
BmsI GCATC 2 cut(s) 292, 582
BmtI GCTAGC 1 cut(s) 407
Bpu1102I GCTNAGC 1 cut(s) 110
BsaBI GATNNNNATC 1 cut(s) 495
BsaHI GRCGYC 1 cut(s) 125
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 348
Bse8I GATNNNNATC 1 cut(s) 495
BseGI GGATG 1 cut(s) 573
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 2 cut(s) 101, 288
BseRI GAGGAG 3 cut(s) 9, 111, 243
BseXI GCAGC 2 cut(s) 283, 507
BseYI CCCAGC 1 cut(s) 314
BshFI GGCC 3 cut(s) 57, 218, 281
BsiHKAI GWGCWC 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 1 cut(s) 339
BsnI GGCC 3 cut(s) 57, 218, 281
Bsp1286I GDGCHC 1 cut(s) 116
Bsp1720I GCTNAGC 1 cut(s) 110
BspANI GGCC 3 cut(s) 57, 218, 281
BspCNI CTCAG 2 cut(s) 102, 287
BspOI GCTAGC 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 348
BssNI GRCGYC 1 cut(s) 125
BssSI CACGAG 1 cut(s) 342
Bst2BI CACGAG 1 cut(s) 342
Bst4CI ACNGT 4 cut(s) 78, 333, 552, 621
Bst6I CTCTTC 2 cut(s) 212, 237
BstACI GRCGYC 1 cut(s) 125
BstAPI GCANNNNNTGC 1 cut(s) 311
BstC8I GCNNGC 4 cut(s) 147, 151, 312, 405
BstDEI CTNAG 3 cut(s) 110, 274, 464
BstF5I GGATG 1 cut(s) 573
BstMAI GTCTC 1 cut(s) 339
BstMWI GCNNNNNNNGC 4 cut(s) 142, 224, 302, 311
BstNSI RCATGY 2 cut(s) 314, 527
BstSFI CTRYAG 1 cut(s) 617
BstV1I GCAGC 2 cut(s) 283, 507
BsuRI GGCC 3 cut(s) 57, 218, 281
BtsCI GGATG 1 cut(s) 573
BtsI GCAGTG 1 cut(s) 305
BtsIMutI CAGTG 2 cut(s) 305, 480
Cac8I GCNNGC 4 cut(s) 147, 151, 312, 405
CaiI CAGNNNCTG 1 cut(s) 479
Cfr13I GGNCC 2 cut(s) 216, 502
CseI GACGC 1 cut(s) 114
Csp6I GTAC 2 cut(s) 438, 553
CviAII CATG 3 cut(s) 59, 311, 524
CviQI GTAC 2 cut(s) 438, 553
DdeI CTNAG 3 cut(s) 110, 274, 464
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 2 cut(s) 212, 237
EarI CTCTTC 2 cut(s) 212, 237
Ecl136II GAGCTC 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 116
Eco47I GGWCC 1 cut(s) 502
Eco53kI GAGCTC 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 116
FaeI CATG 3 cut(s) 62, 314, 527
FaiI YATR 6 cut(s) 14, 60, 266, 312, 489, 525
FatI CATG 3 cut(s) 58, 310, 523
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 2 cut(s) 297, 521
FokI GGATG 1 cut(s) 560
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 2 cut(s) 297, 521
FspBI CTAG 1 cut(s) 404
GluI GCNGC 2 cut(s) 297, 521
GsaI CCCAGC 1 cut(s) 318
HaeIII GGCC 3 cut(s) 57, 218, 281
HgaI GACGC 1 cut(s) 114
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 3 cut(s) 62, 314, 527
HinfI GANTC 1 cut(s) 8
Hpy166II GTNNAC 2 cut(s) 329, 448
Hpy188I TCNGA 2 cut(s) 50, 277
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 2 cut(s) 329, 448
Hpy99I CGWCG 2 cut(s) 27, 124
HpyAV CCTTC 1 cut(s) 286
HpyCH4III ACNGT 4 cut(s) 78, 333, 552, 621
HpyCH4V TGCA 5 cut(s) 305, 310, 341, 523, 595
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 224, 302, 311
HpyF3I CTNAG 3 cut(s) 110, 274, 464
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 3 cut(s) 62, 314, 527
LpnPI CCDG 9 cut(s) 135, 199, 273, 300, 368, 485, 492, 520, 541
Lsp1109I GCAGC 2 cut(s) 283, 507
LweI GCATC 2 cut(s) 292, 582
MaeI CTAG 1 cut(s) 404
MboII GAAGA 6 cut(s) 94, 199, 224, 371, 418, 622
MhlI GDGCHC 1 cut(s) 116
MlsI TGGCCA 1 cut(s) 57
MluCI AATT 3 cut(s) 364, 561, 585
MluNI TGGCCA 1 cut(s) 57
MnlI CCTC 9 cut(s) 27, 30, 44, 89, 215, 221, 261, 264, 271
Mox20I TGGCCA 1 cut(s) 57
MscI TGGCCA 1 cut(s) 57
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 1 cut(s) 510
Msp20I TGGCCA 1 cut(s) 57
MwoI GCNNNNNNNGC 4 cut(s) 142, 224, 302, 311
NheI GCTAGC 1 cut(s) 403
NlaIII CATG 3 cut(s) 62, 314, 527
NspI RCATGY 2 cut(s) 314, 527
PaeI GCATGC 1 cut(s) 314
PfeI GAWTC 1 cut(s) 8
PflFI GACNNNGTC 1 cut(s) 124
PkrI GCNGC 2 cut(s) 298, 522
PshBI ATTAAT 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 116
PspFI CCCAGC 1 cut(s) 314
PspPI GGNCC 2 cut(s) 216, 502
PstNI CAGNNNCTG 1 cut(s) 479
PsyI GACNNNGTC 1 cut(s) 124
RsaI GTAC 2 cut(s) 439, 554
RsaNI GTAC 2 cut(s) 438, 553
RseI CAYNNNNRTG 1 cut(s) 510
SacI GAGCTC 1 cut(s) 116
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 2 cut(s) 297, 521
Sau96I GGNCC 2 cut(s) 216, 502
SduI GDGCHC 1 cut(s) 116
SetI ASST 9 cut(s) 46, 55, 116, 151, 247, 387, 409, 522, 618
SfaNI GCATC 2 cut(s) 292, 582
SfcI CTRYAG 1 cut(s) 617
SinI GGWCC 1 cut(s) 502
SmiMI CAYNNNNRTG 1 cut(s) 510
SphI GCATGC 1 cut(s) 314
Sse9I AATT 3 cut(s) 364, 561, 585
SspMI CTAG 1 cut(s) 404
SstI GAGCTC 1 cut(s) 116
TaaI ACNGT 4 cut(s) 78, 333, 552, 621
TasI AATT 3 cut(s) 364, 561, 585
TatI WGTACW 1 cut(s) 552
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 2 cut(s) 312, 480
TseI GCWGC 2 cut(s) 296, 520
TspDTI ATGAA 1 cut(s) 26
TspGWI ACGGA 2 cut(s) 22, 425
TspRI CASTG 2 cut(s) 312, 480
Tth111I GACNNNGTC 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 502
VspI ATTAAT 1 cut(s) 321
XapI RAATTY 1 cut(s) 585
XceI RCATGY 2 cut(s) 314, 527
XmiI GTMKAC 1 cut(s) 447
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.