pycom05g11200
ERF Family

Belongs to the methyltransferase superfamily. METTL16 RlmF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009335
Physical Location & Seq
Reverse (-)
14701059 .. 14704727
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g11200.3

Sequence Viewer

Length: 1494 bp
ATGATTCACTCTCAAATGTCGATGAACAAAAATAAGAGGAGGAAGAGAAAGGAGGAGCGACCTGCGACCCACCTGAGAAACAAATACTCCGAGAACCCACCCGACTTCGCTCAATTGGCTTCTTTGTACCCCTCTTTCGAGCCCTTCGTCTTCTTCTCCCGAGACGGTCGGCCCAGAATTGACTGGACCGACTTCAACGCCACCCGGGAACTCACTCGGGTCCTCCTCCTCCACGACCACCGCCTCATCTGGTGGATTCCAGATGGGCAGCTGTGCCCCACTGTGCCCAATAGATCGAATTACATTCATTTGATTGAAGATCTTCTCTCATCTGACATCATTGTGAAGACTACAAGCAATGGTGATAAAGTTAGGGGCTTTGATATTGGAACTGGAGCAAACTGCATATATCCGCTTCTTGGTGCTTCTCTTCTGGGTTGGAGCTTTGTTGGGTCAGATGTGAGTGATGTAGCACTAGAGTGGGCAGAAAAAAATGTTAGAGATAATCCACATATTGCGGAACTTATTGAAATTAGAAAGGTTGAAAGTGTTAAAGAGACCCTTCCACTGGAAGGATTGCCAAATGGGATGTTGGATAGTACTGAAAGCAAAATAGAATTGACTGAGAATATGGAAAGGGAAGCAGTGCCTTTGCCCTCCTCTTCATCCACATATCACGGGCCAGCTATTCTCCTTGGTGTGGTTAAGGATGGTGAGGAGTTTGACTTCTGCATGTGTAACCCTCCATTTTTCGAGACCATGGAGGAAGCAGGACTGAATCCAAAAACTGCATGTGGTGGGACCCCAACCGAGATGGTTTGTCCTGGTGGAGAAAAGGCTTTCATCACTCGCATTATTGAAGATAGCGTCACATTGAAGAATACTTTTCGGTGGTACACATCAATGGTTGGTAGGAAACTGAATATCAAAATCCTAACATCAAAGCTTTGGGAAGTTGGAGTCACCATGGTAAAGACAACTGAATTTGTCCAAGGCCAAACTTCTCGATGGGGGCTGGCCTGGTCCTTTTTGCCTCCTGCCAAGCAGTTAGTATCATCTCATGTGGCTGTGAAGAACAACCTCTCTTTCATGCTTGAGGGCCTCGACCGAAAATTTGGTGCTATACATGTATTGGAATCAACCAAATCCTTTTTCAGCTGTGGTGGTGCATCGTGTAACTTGAACACATCCTCATTTACACTCGATATCACCATGACAAAAGATCACTCAGATGCAATCTTGAAGGGTGGGCCACAAGATTATGATAGAGTTATCAGTTGTGAAGATAAGCAAGAGGCATCCACAAGCTCAAGCTGTTTGAACTTGCCTTCAAATAATCTAGGTTTTCGTGTTTCGGTCTTTCAGCAAAACCCTGGGACACTTCTGGTGAAAGGCTCACTACAGCAGAGAGATTGTCAACTTTCAGGAGCATTCTCACCAATATTCCAACGACTTGAGGAAGATCTGAAACAGAAATTTTGTAGAAGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000154 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008174 GO:0008649 GO:0008757 GO:0008988 GO:0009056 GO:0009057 GO:0009451 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016422 GO:0016433 GO:0016556 GO:0016740 GO:0016741 GO:0017069 GO:0017070 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0030629 GO:0031167 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032259 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0035613 GO:0040031 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043484 GO:0043487 GO:0043488 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0045935 GO:0046483 GO:0046700 GO:0048024 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050684 GO:0050779 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0052907 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0065007 GO:0065008 GO:0070475 GO:0071704 GO:0071840 GO:0080009 GO:0080090 GO:0090304 GO:0097159 GO:0120048 GO:0120049 GO:0140098 GO:0140102 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903311 GO:1903313 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_10

Protein Analysis

498

Amino Acids

55.74

Weight (kDa)

6.4

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 70
AciI CCGC 3 cut(s) 241, 413, 518
AcsI RAATTY 3 cut(s) 983, 1112, 1475
AfaI GTAC 3 cut(s) 128, 601, 896
AfiI CCNNNNNNNGG 4 cut(s) 419, 568, 572, 700
AflIII ACRYGT 1 cut(s) 1126
AjnI CCWGG 3 cut(s) 823, 1019, 1372
AjuI GAANNNNNNNTTGG 2 cut(s) 1136, 1168
AleI CACNNNNGTG 1 cut(s) 478
AluBI AGCT 7 cut(s) 271, 444, 686, 946, 1158, 1308, 1314
AluI AGCT 7 cut(s) 271, 444, 686, 946, 1158, 1308, 1314
Alw26I GTCTC 3 cut(s) 156, 551, 749
Ama87I CYCGRG 3 cut(s) 159, 204, 216
AoxI GGCC 6 cut(s) 170, 680, 994, 1017, 1099, 1250
ApeKI GCWGC 1 cut(s) 268
ApoI RAATTY 3 cut(s) 983, 1112, 1475
ArsI GACNNNNNNTTYG 2 cut(s) 1097, 1129
Asp700I GAANNNNTTC 1 cut(s) 321
AspS9I GGNCC 8 cut(s) 171, 186, 220, 680, 801, 1023, 1099, 1250
AsuC2I CCSGG 2 cut(s) 205, 206
AsuHPI GGTGA 6 cut(s) 374, 725, 955, 1201, 1399, 1428
AvaI CYCGRG 3 cut(s) 159, 204, 216
AvaII GGWCC 4 cut(s) 186, 220, 801, 1023
BaeGI GKGCMC 2 cut(s) 278, 288
BanII GRGCYC 1 cut(s) 144
BbsI GAAGAC 2 cut(s) 142, 353
BbvI GCAGC 1 cut(s) 280
BccI CCATC 4 cut(s) 257, 704, 808, 1002
BciT130I CCWGG 3 cut(s) 825, 1021, 1374
BcnI CCSGG 2 cut(s) 205, 206
BcoDI GTCTC 3 cut(s) 156, 551, 749
BfaI CTAG 2 cut(s) 476, 1340
BfmI CTRYAG 1 cut(s) 1400
BfuAI ACCTGC 1 cut(s) 70
BglII AGATCT 2 cut(s) 319, 1462
BisI GCNGC 1 cut(s) 269
BlsI GCNGC 1 cut(s) 270
BmcAI AGTACT 1 cut(s) 601
Bme1390I CCNGG 5 cut(s) 205, 206, 825, 1021, 1374
Bme18I GGWCC 4 cut(s) 186, 220, 801, 1023
BmeT110I CYCGRG 3 cut(s) 159, 204, 216
BmgT120I GGNCC 8 cut(s) 171, 186, 220, 680, 801, 1023, 1099, 1250
BmiI GGNNCC 3 cut(s) 221, 802, 803
BmrFI CCNGG 5 cut(s) 205, 206, 825, 1021, 1374
BmsI GCATC 3 cut(s) 1178, 1222, 1307
BpiI GAAGAC 2 cut(s) 142, 353
BpmI CTGGAG 1 cut(s) 414
BpuEI CTTGAG 3 cut(s) 1115, 1294, 1475
BpuMI CCSGG 2 cut(s) 205, 206
BsaI GGTCTC 2 cut(s) 551, 749
BsaJI CCNNGG 7 cut(s) 204, 694, 759, 966, 991, 1372, 1373
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 4 cut(s) 419, 568, 572, 700
Bse1I ACTGG 3 cut(s) 188, 397, 573
Bse3DI GCAATG 1 cut(s) 364
BseBI CCWGG 3 cut(s) 825, 1021, 1374
BseDI CCNNGG 7 cut(s) 204, 694, 759, 966, 991, 1372, 1373
BseGI GGATG 5 cut(s) 594, 665, 715, 1187, 1298
BseLI CCNNNNNNNGG 4 cut(s) 419, 568, 572, 700
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 3 cut(s) 65, 615, 1242
BseNI ACTGG 3 cut(s) 188, 397, 573
BseRI GAGGAG 6 cut(s) 52, 68, 215, 218, 649, 731
BseSI GKGCMC 2 cut(s) 278, 288
BseXI GCAGC 1 cut(s) 280
Bsh1285I CGRYCG 2 cut(s) 169, 1108
BshFI GGCC 6 cut(s) 172, 682, 996, 1019, 1101, 1252
BsiEI CGRYCG 2 cut(s) 169, 1108
BsiHKCI CYCGRG 3 cut(s) 159, 204, 216
BsiSI CCGG 1 cut(s) 205
BslFI GGGAC 2 cut(s) 814, 1390
BslI CCNNNNNNNGG 4 cut(s) 419, 568, 572, 700
BsmAI GTCTC 3 cut(s) 156, 551, 749
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 2 cut(s) 814, 1390
BsmI GAATGC 1 cut(s) 1430
BsnI GGCC 6 cut(s) 172, 682, 996, 1019, 1101, 1252
Bso31I GGTCTC 2 cut(s) 551, 749
BsoBI CYCGRG 3 cut(s) 159, 204, 216
Bsp1286I GDGCHC 3 cut(s) 144, 278, 288
Bsp143I GATC 4 cut(s) 293, 319, 1222, 1462
Bsp19I CCATGG 2 cut(s) 759, 966
BspACI CCGC 3 cut(s) 241, 413, 518
BspANI GGCC 6 cut(s) 172, 682, 996, 1019, 1101, 1252
BspCNI CTCAG 3 cut(s) 66, 616, 1241
BspLI GGNNCC 3 cut(s) 221, 802, 803
BspMI ACCTGC 1 cut(s) 70
BspTNI GGTCTC 2 cut(s) 551, 749
BsrDI GCAATG 1 cut(s) 364
BsrI ACTGG 3 cut(s) 188, 397, 573
BssECI CCNNGG 7 cut(s) 204, 694, 759, 966, 991, 1372, 1373
BssMI GATC 4 cut(s) 293, 319, 1222, 1462
BssT1I CCWWGG 4 cut(s) 694, 759, 966, 991
Bst2UI CCWGG 3 cut(s) 825, 1021, 1374
Bst4CI ACNGT 2 cut(s) 167, 283
Bst6I CTCTTC 3 cut(s) 38, 435, 667
BstC8I GCNNGC 2 cut(s) 684, 1017
BstDEI CTNAG 3 cut(s) 74, 624, 1228
BstDSI CCRYGG 2 cut(s) 759, 966
BstF5I GGATG 5 cut(s) 594, 665, 715, 1187, 1298
BstKTI GATC 4 cut(s) 296, 322, 1225, 1465
BstMAI GTCTC 3 cut(s) 156, 551, 749
BstMBI GATC 4 cut(s) 293, 319, 1222, 1462
BstMCI CGRYCG 2 cut(s) 169, 1108
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 3 cut(s) 825, 1021, 1374
BstNSI RCATGY 3 cut(s) 736, 795, 1130
BstSCI CCNGG 5 cut(s) 203, 204, 823, 1019, 1372
BstSFI CTRYAG 1 cut(s) 1400
BstSLI GKGCMC 2 cut(s) 278, 288
BstV1I GCAGC 1 cut(s) 280
BstV2I GAAGAC 2 cut(s) 142, 353
BstX2I RGATCY 2 cut(s) 319, 1462
BstYI RGATCY 2 cut(s) 319, 1462
BsuRI GGCC 6 cut(s) 172, 682, 996, 1019, 1101, 1252
BtgI CCRYGG 2 cut(s) 759, 966
BtsCI GGATG 5 cut(s) 594, 665, 715, 1187, 1298
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 3 cut(s) 279, 566, 651
BveI ACCTGC 1 cut(s) 70
Cac8I GCNNGC 2 cut(s) 684, 1017
Cfr13I GGNCC 8 cut(s) 171, 186, 220, 680, 801, 1023, 1099, 1250
Cfr9I CCCGGG 1 cut(s) 204
CseI GACGC 1 cut(s) 856
Csp6I GTAC 3 cut(s) 127, 600, 895
CviAII CATG 8 cut(s) 733, 760, 792, 967, 1061, 1090, 1127, 1213
CviQI GTAC 3 cut(s) 127, 600, 895
DdeI CTNAG 3 cut(s) 74, 624, 1228
DpnI GATC 4 cut(s) 295, 321, 1224, 1464
DpnII GATC 4 cut(s) 293, 319, 1222, 1462
Eam1104I CTCTTC 3 cut(s) 38, 435, 667
EarI CTCTTC 3 cut(s) 38, 435, 667
Eco130I CCWWGG 4 cut(s) 694, 759, 966, 991
Eco24I GRGCYC 1 cut(s) 144
Eco31I GGTCTC 2 cut(s) 551, 749
Eco32I GATATC 1 cut(s) 1207
Eco47I GGWCC 4 cut(s) 186, 220, 801, 1023
Eco88I CYCGRG 3 cut(s) 159, 204, 216
EcoO109I RGGNCCY 3 cut(s) 220, 801, 1099
EcoRII CCWGG 3 cut(s) 823, 1019, 1372
EcoRV GATATC 1 cut(s) 1207
EcoT14I CCWWGG 4 cut(s) 694, 759, 966, 991
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 4 cut(s) 694, 759, 966, 991
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 8 cut(s) 736, 763, 795, 970, 1064, 1093, 1130, 1216
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FaqI GGGAC 2 cut(s) 814, 1390
FatI CATG 8 cut(s) 732, 759, 791, 966, 1060, 1089, 1126, 1212
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 5 cut(s) 601, 652, 722, 1174, 1285
FriOI GRGCYC 1 cut(s) 144
Fsp4HI GCNGC 1 cut(s) 269
FspBI CTAG 2 cut(s) 476, 1340
GluI GCNGC 1 cut(s) 269
GsuI CTGGAG 1 cut(s) 414
HaeIII GGCC 6 cut(s) 172, 682, 996, 1019, 1101, 1252
HapII CCGG 1 cut(s) 205
HgaI GACGC 1 cut(s) 856
Hin1II CATG 8 cut(s) 736, 763, 795, 970, 1064, 1093, 1130, 1216
HincII GTYRAC 1 cut(s) 1418
HindII GTYRAC 1 cut(s) 1418
HindIII AAGCTT 1 cut(s) 944
HinfI GANTC 5 cut(s) 4, 256, 778, 960, 1136
HpaII CCGG 1 cut(s) 205
HphI GGTGA 6 cut(s) 374, 725, 955, 1201, 1399, 1428
Hpy166II GTNNAC 2 cut(s) 897, 1418
Hpy188I TCNGA 5 cut(s) 91, 334, 457, 1231, 1467
Hpy188III TCNNGA 6 cut(s) 159, 260, 754, 1005, 1240, 1425
Hpy8I GTNNAC 2 cut(s) 897, 1418
HpyAV CCTTC 6 cut(s) 154, 566, 572, 1237, 1338, 1479
HpyCH4III ACNGT 2 cut(s) 167, 283
HpyCH4V TGCA 5 cut(s) 405, 732, 791, 1169, 1235
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 3 cut(s) 74, 624, 1228
Hsp92II CATG 8 cut(s) 736, 763, 795, 970, 1064, 1093, 1130, 1216
KflI GGGWCCC 1 cut(s) 801
Kzo9I GATC 4 cut(s) 293, 319, 1222, 1462
LmnI GCTCC 4 cut(s) 55, 395, 441, 1427
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 3 cut(s) 1178, 1222, 1307
MaeI CTAG 2 cut(s) 476, 1340
MaeIII GTNAC 4 cut(s) 737, 868, 961, 1175
MalI GATC 4 cut(s) 295, 321, 1224, 1464
MboI GATC 4 cut(s) 293, 319, 1222, 1462
MfeI CAATTG 1 cut(s) 113
MflI RGATCY 2 cut(s) 319, 1462
MhlI GDGCHC 3 cut(s) 144, 278, 288
MluCI AATT 8 cut(s) 113, 177, 298, 531, 617, 983, 1112, 1475
MlyI GAGTC 1 cut(s) 969
MmeI TCCRAC 4 cut(s) 419, 573, 937, 1471
MroXI GAANNNNTTC 1 cut(s) 321
MseI TTAA 2 cut(s) 552, 705
MslI CAYNNNNRTG 4 cut(s) 341, 478, 902, 1230
MspA1I CMGCKG 2 cut(s) 271, 1158
MspI CCGG 1 cut(s) 205
MspR9I CCNGG 5 cut(s) 205, 206, 825, 1021, 1374
MunI CAATTG 1 cut(s) 113
Mva1269I GAATGC 1 cut(s) 1430
MvaI CCWGG 3 cut(s) 825, 1021, 1374
MwoI GCNNNNNNNGC 1 cut(s) 116
NciI CCSGG 2 cut(s) 205, 206
NcoI CCATGG 2 cut(s) 759, 966
NdeII GATC 4 cut(s) 293, 319, 1222, 1462
NlaIII CATG 8 cut(s) 736, 763, 795, 970, 1064, 1093, 1130, 1216
NlaIV GGNNCC 3 cut(s) 221, 802, 803
NmuCI GTSAC 2 cut(s) 868, 961
NspI RCATGY 3 cut(s) 736, 795, 1130
OliI CACNNNNGTG 1 cut(s) 478
PasI CCCWGGG 1 cut(s) 1373
PciI ACATGT 1 cut(s) 1126
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PctI GAATGC 1 cut(s) 1430
PdmI GAANNNNTTC 1 cut(s) 321
PfeI GAWTC 4 cut(s) 4, 256, 778, 1136
PkrI GCNGC 1 cut(s) 270
PleI GAGTC 1 cut(s) 968
PpsI GAGTC 1 cut(s) 968
PpuMI RGGWCCY 2 cut(s) 220, 801
PscI ACATGT 1 cut(s) 1126
Psp5II RGGWCCY 2 cut(s) 220, 801
Psp6I CCWGG 3 cut(s) 823, 1019, 1372
PspGI CCWGG 3 cut(s) 823, 1019, 1372
PspN4I GGNNCC 3 cut(s) 221, 802, 803
PspPI GGNCC 8 cut(s) 171, 186, 220, 680, 801, 1023, 1099, 1250
PspPPI RGGWCCY 2 cut(s) 220, 801
PsuI RGATCY 2 cut(s) 319, 1462
PvuII CAGCTG 2 cut(s) 271, 1158
RsaI GTAC 3 cut(s) 128, 601, 896
RsaNI GTAC 3 cut(s) 127, 600, 895
RseI CAYNNNNRTG 4 cut(s) 341, 478, 902, 1230
SaqAI TTAA 2 cut(s) 552, 705
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 4 cut(s) 293, 319, 1222, 1462
Sau96I GGNCC 8 cut(s) 171, 186, 220, 680, 801, 1023, 1099, 1250
ScaI AGTACT 1 cut(s) 601
SchI GAGTC 1 cut(s) 969
ScrFI CCNGG 5 cut(s) 205, 206, 825, 1021, 1374
SduI GDGCHC 3 cut(s) 144, 278, 288
SfaNI GCATC 3 cut(s) 1178, 1222, 1307
SfcI CTRYAG 1 cut(s) 1400
SinI GGWCC 4 cut(s) 186, 220, 801, 1023
SmaI CCCGGG 1 cut(s) 206
SmiMI CAYNNNNRTG 4 cut(s) 341, 478, 902, 1230
SmlI CTYRAG 3 cut(s) 1094, 1309, 1454
SmoI CTYRAG 3 cut(s) 1094, 1309, 1454
Sse9I AATT 8 cut(s) 113, 177, 298, 531, 617, 983, 1112, 1475
SsiI CCGC 3 cut(s) 241, 413, 518
SspI AATATT 1 cut(s) 1443
SspMI CTAG 2 cut(s) 476, 1340
StyD4I CCNGG 5 cut(s) 203, 204, 823, 1019, 1372
StyI CCWWGG 4 cut(s) 694, 759, 966, 991
TaaI ACNGT 2 cut(s) 167, 283
TaqI TCGA 7 cut(s) 20, 138, 296, 753, 1006, 1104, 1203
TaqII GACCGA 3 cut(s) 203, 1122, 1345
TasI AATT 8 cut(s) 113, 177, 298, 531, 617, 983, 1112, 1475
TatI WGTACW 1 cut(s) 599
TfiI GAWTC 4 cut(s) 4, 256, 778, 1136
Tru1I TTAA 2 cut(s) 552, 705
Tru9I TTAA 2 cut(s) 552, 705
TscAI CASTG 3 cut(s) 286, 573, 651
TseFI GTSAC 2 cut(s) 868, 961
TseI GCWGC 1 cut(s) 268
Tsp45I GTSAC 2 cut(s) 868, 961
TspDTI ATGAA 5 cut(s) 38, 296, 654, 832, 1078
TspMI CCCGGG 1 cut(s) 204
TspRI CASTG 3 cut(s) 286, 573, 651
VpaK11BI GGWCC 4 cut(s) 186, 220, 801, 1023
XapI RAATTY 3 cut(s) 983, 1112, 1475
XceI RCATGY 3 cut(s) 736, 795, 1130
XmaI CCCGGG 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 2 cut(s) 476, 1340
ZrmI AGTACT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.