RchiOBHm_Chr6g0244441
ERF Family

Belongs to the methyltransferase superfamily. METTL16 RlmF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009335
Physical Location & Seq
Forward (+)
513485 .. 518212
4728 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21908

Sequence Viewer

Length: 1467 bp
ATGGGATCAAATAAGAATAAGAAGAGGAGGAGGACTGCGGAGCGACCCACGACCCACCCGAGAAACAAATACTCCAATAACCCGCCCGACTTTGCCGAACTAGCTTCTCTCTACCCGACTTTCAAACCTTTCGTTTTCTTTGGCCGCGATGGCCGACCCAGAATCGACTGGACCGACTTCAACGCCACCCGGGAACTCACCCGGGTCCTGCTCCTCCATGACCACTGCCTCAACTGGTGGATTCCAGATGGTCAGCTATGCCCCACGGTACCCAATAGATCGAACTACATTCATTGGATTGAAGATCTCCTGTCGTCTGACATTATTGCCAAGACTACAAAAATTGGTGATAAAGTGAGGGGGTTTGATATAGGAACTGGAGCAAACTGCATATATCCACTTCTTGGTGCATCTCTTCTAGGGTGGAGCTTTGTTGGGTCAGATATGACTGATGTAGCGCTAGAGTGGGCTGAAAAGAATGTTAGAGATAATCCACATATCTCGGAACTTATTGAAATTCGGAAGGTAGAAAGTGGTGAAAACACCCTTCCAGCAGAAGGATCAAATAATGAGGCATTGGTTAGTACCAAAACCGAAATATATCTGACTGAGGATATGGGAAGGGAAGGGAATAGTCTGAATACAGGGTATTATGGGCCACCTATTCTTCTTGGAGTGGTCAGGGATGGTGAGGAGTTTGACTTCTGCATGTGTAACCCTCCATTTTTTGAGACAATGGAAGAAGCAGGACTCAATCCAAAAACTTCATGTGGTGGTACCCCAGCTGAGATGATTTGTCCTGGTGGGGAAAAGGCATTTGTCACTTGCATTATAGAAGATAGTGTCACGTTGAGGCATACTTTTCGGTGGTACACATCAATGCTAGGGAGGAAATCAAATCTCAAATTACTAACATCAAAGCTTTGGGAAGTTGGAGCCACCGTAGTGAAGACAACTGAATTTGTCCAAGGCCAAACATGTCGATGGGGGTTGGCCTGGTCCTTTTTACCTTCTGTTAAGAAGATATTATCATCTCATGTGACCGAAAAGAGTAACCTTTCCTTCATGCTTCAGGGCCTTGAACGGAAACTAGGTGCTATACACGTCTTGCAGTCAGTTGAAGACTATTTCCGCAATGCTGGTGCATTGTGTAAATTGAACACATCCTCATTTACAGTTGATATCACTGCAAAAGATGATAAGTCAGATGCAAACTTGAAAAGTGAGTCACAGAATTATGATGGCGTTGCAAGTTGTGATGATGTGCAAGAGGCATCTGGATCAAGCTGTTTGAATTTGCTGTCAAATAACCTAAGTTTCCGTGTTTCGGTCTTTCAGCAAACCCCTGGCACACTTCTAGTGAAAGGCTCACTACAGCAGAGAGATGGTCAAATTTCAGGATCATTCTCACCGATATTTCGAAGGTTGGAGGAAGTTCTGAAACAGAAGTTCCATAGAGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000154 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008174 GO:0008649 GO:0008757 GO:0008988 GO:0009056 GO:0009057 GO:0009451 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016422 GO:0016433 GO:0016556 GO:0016740 GO:0016741 GO:0017069 GO:0017070 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0030629 GO:0031167 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032259 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0035613 GO:0040031 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043484 GO:0043487 GO:0043488 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0045935 GO:0046483 GO:0046700 GO:0048024 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050684 GO:0050779 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0052907 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0065007 GO:0065008 GO:0070475 GO:0071704 GO:0071840 GO:0080009 GO:0080090 GO:0090304 GO:0097159 GO:0120048 GO:0120049 GO:0140098 GO:0140102 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903311 GO:1903313 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_10

Protein Analysis

488

Amino Acids

54.6

Weight (kDa)

6.35

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_10 PF05971 14 - 183 9.4e-50 RNA methyltransferase
Methyltransf_10 PF05971 222 - 336 1.7e-26 RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 268, 776
AccB1I GGYRCC 2 cut(s) 268, 776
AccB7I CCANNNNNTGG 1 cut(s) 404
AccII CGCG 1 cut(s) 147
AciI CCGC 4 cut(s) 38, 83, 145, 1132
AclWI GGATC 4 cut(s) 13, 568, 1288, 1408
AcoI YGGCCR 2 cut(s) 142, 151
AcsI RAATTY 4 cut(s) 516, 959, 1294, 1392
AcuI CTGAAG 1 cut(s) 1055
AfaI GTAC 4 cut(s) 270, 586, 778, 872
AfeI AGCGCT 1 cut(s) 459
AfiI CCNNNNNNNGG 3 cut(s) 404, 557, 1327
AflIII ACRYGT 2 cut(s) 977, 1102
AgsI TTSAA 9 cut(s) 124, 181, 302, 515, 1082, 1121, 1159, 1219, 1294
AjiI CACGTC 1 cut(s) 1105
AjnI CCWGG 3 cut(s) 799, 995, 1345
AleI CACNNNNGTG 1 cut(s) 944
AluBI AGCT 6 cut(s) 104, 256, 429, 785, 922, 1287
AluI AGCT 6 cut(s) 104, 256, 429, 785, 922, 1287
Alw26I GTCTC 1 cut(s) 725
AlwI GGATC 4 cut(s) 13, 568, 1288, 1408
Ama87I CYCGRG 3 cut(s) 58, 189, 201
Aor51HI AGCGCT 1 cut(s) 459
AoxI GGCC 6 cut(s) 142, 151, 656, 970, 993, 1075
ApoI RAATTY 4 cut(s) 516, 959, 1294, 1392
Asp718I GGTACC 2 cut(s) 268, 776
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 5 cut(s) 171, 205, 656, 999, 1075
AsuC2I CCSGG 4 cut(s) 190, 191, 202, 203
AsuHPI GGTGA 5 cut(s) 190, 359, 548, 701, 1401
AsuII TTCGAA 1 cut(s) 1420
AvaI CYCGRG 3 cut(s) 58, 189, 201
AvaII GGWCC 3 cut(s) 171, 205, 999
BanI GGYRCC 2 cut(s) 268, 776
BbsI GAAGAC 2 cut(s) 956, 1128
BccI CCATC 6 cut(s) 143, 242, 680, 978, 1235, 1379
BcgI CGANNNNNNTGC 2 cut(s) 845, 879
BciT130I CCWGG 3 cut(s) 801, 997, 1347
BcnI CCSGG 4 cut(s) 190, 191, 202, 203
BcoDI GTCTC 1 cut(s) 725
BfaI CTAG 6 cut(s) 101, 419, 461, 884, 1091, 1358
BfmI CTRYAG 1 cut(s) 1373
BfoI RGCGCY 1 cut(s) 461
BglI GCCNNNNNGGC 1 cut(s) 150
BglII AGATCT 1 cut(s) 304
BisI GCNGC 1 cut(s) 145
BlsI GCNGC 1 cut(s) 146
Bme1390I CCNGG 7 cut(s) 190, 191, 202, 203, 801, 997, 1347
Bme18I GGWCC 3 cut(s) 171, 205, 999
BmeT110I CYCGRG 3 cut(s) 58, 189, 201
BmgBI CACGTC 1 cut(s) 1105
BmgT120I GGNCC 5 cut(s) 171, 205, 656, 999, 1075
BmiI GGNNCC 4 cut(s) 206, 270, 778, 937
BmrFI CCNGG 7 cut(s) 190, 191, 202, 203, 801, 997, 1347
BmsI GCATC 3 cut(s) 419, 1198, 1283
BpiI GAAGAC 2 cut(s) 956, 1128
BpmI CTGGAG 1 cut(s) 399
Bpu14I TTCGAA 1 cut(s) 1420
BpuMI CCSGG 4 cut(s) 190, 191, 202, 203
BsaJI CCNNGG 5 cut(s) 189, 201, 264, 967, 1345
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 927, 957
Bsc4I CCNNNNNNNGG 3 cut(s) 404, 557, 1327
Bse1I ACTGG 3 cut(s) 173, 239, 382
Bse3DI GCAATG 1 cut(s) 1141
BseBI CCWGG 3 cut(s) 801, 997, 1347
BseDI CCNNGG 5 cut(s) 189, 201, 264, 967, 1345
BseGI GGATG 2 cut(s) 691, 1163
BseLI CCNNNNNNNGG 3 cut(s) 404, 557, 1327
BseMI GCAATG 1 cut(s) 1141
BseMII CTCAG 2 cut(s) 600, 777
BseNI ACTGG 3 cut(s) 173, 239, 382
BseRI GAGGAG 4 cut(s) 40, 43, 203, 707
BseYI CCCAGC 1 cut(s) 781
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 6 cut(s) 144, 153, 658, 972, 995, 1077
BshNI GGYRCC 2 cut(s) 268, 776
BsiHKCI CYCGRG 3 cut(s) 58, 189, 201
BsiSI CCGG 2 cut(s) 190, 202
BslI CCNNNNNNNGG 3 cut(s) 404, 557, 1327
BsmAI GTCTC 1 cut(s) 725
BsnI GGCC 6 cut(s) 144, 153, 658, 972, 995, 1077
BsoBI CYCGRG 3 cut(s) 58, 189, 201
Bsp119I TTCGAA 1 cut(s) 1420
Bsp143I GATC 6 cut(s) 5, 278, 304, 560, 1280, 1400
BspACI CCGC 4 cut(s) 38, 83, 145, 1132
BspANI GGCC 6 cut(s) 144, 153, 658, 972, 995, 1077
BspCNI CTCAG 2 cut(s) 601, 778
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 4 cut(s) 206, 270, 778, 937
BspPI GGATC 4 cut(s) 13, 568, 1288, 1408
BspT104I TTCGAA 1 cut(s) 1420
BspT107I GGYRCC 2 cut(s) 268, 776
BsrDI GCAATG 1 cut(s) 1141
BsrI ACTGG 3 cut(s) 173, 239, 382
BssECI CCNNGG 5 cut(s) 189, 201, 264, 967, 1345
BssMI GATC 6 cut(s) 5, 278, 304, 560, 1280, 1400
BssT1I CCWWGG 1 cut(s) 967
Bst2UI CCWGG 3 cut(s) 801, 997, 1347
Bst4CI ACNGT 3 cut(s) 268, 943, 1177
Bst6I CTCTTC 2 cut(s) 17, 420
BstBI TTCGAA 1 cut(s) 1420
BstDEI CTNAG 3 cut(s) 609, 786, 1313
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 2 cut(s) 691, 1163
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 461
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 6 cut(s) 8, 281, 307, 563, 1283, 1403
BstMAI GTCTC 1 cut(s) 725
BstMBI GATC 6 cut(s) 5, 278, 304, 560, 1280, 1400
BstMWI GCNNNNNNNGC 2 cut(s) 101, 150
BstNI CCWGG 3 cut(s) 801, 997, 1347
BstNSI RCATGY 2 cut(s) 712, 981
BstSCI CCNGG 7 cut(s) 188, 189, 200, 201, 799, 995, 1345
BstSFI CTRYAG 1 cut(s) 1373
BstUI CGCG 1 cut(s) 147
BstV2I GAAGAC 2 cut(s) 956, 1128
BstX2I RGATCY 1 cut(s) 304
BstYI RGATCY 1 cut(s) 304
BsuRI GGCC 6 cut(s) 144, 153, 658, 972, 995, 1077
BtgI CCRYGG 1 cut(s) 264
BtgZI GCGATG 1 cut(s) 162
BtrI CACGTC 1 cut(s) 1105
BtsCI GGATG 2 cut(s) 691, 1163
BtsI GCAGTG 2 cut(s) 223, 1185
BtsIMutI CAGTG 2 cut(s) 223, 1185
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 5 cut(s) 171, 205, 656, 999, 1075
Cfr9I CCCGGG 2 cut(s) 189, 201
Csp6I GTAC 4 cut(s) 269, 585, 777, 871
CviAII CATG 6 cut(s) 218, 709, 768, 978, 1037, 1066
CviQI GTAC 4 cut(s) 269, 585, 777, 871
DdeI CTNAG 3 cut(s) 609, 786, 1313
DpnI GATC 6 cut(s) 7, 280, 306, 562, 1282, 1402
DpnII GATC 6 cut(s) 5, 278, 304, 560, 1280, 1400
EaeI YGGCCR 2 cut(s) 142, 151
Eam1104I CTCTTC 2 cut(s) 17, 420
EarI CTCTTC 2 cut(s) 17, 420
Eco130I CCWWGG 1 cut(s) 967
Eco32I GATATC 1 cut(s) 1183
Eco47I GGWCC 3 cut(s) 171, 205, 999
Eco47III AGCGCT 1 cut(s) 459
Eco57I CTGAAG 1 cut(s) 1055
Eco88I CYCGRG 3 cut(s) 58, 189, 201
EcoO109I RGGNCCY 2 cut(s) 205, 1075
EcoRII CCWGG 3 cut(s) 799, 995, 1345
EcoRV GATATC 1 cut(s) 1183
EcoT14I CCWWGG 1 cut(s) 967
ErhI CCWWGG 1 cut(s) 967
FaeI CATG 6 cut(s) 221, 712, 771, 981, 1040, 1069
FatI CATG 6 cut(s) 217, 708, 767, 977, 1036, 1065
FauI CCCGC 1 cut(s) 90
Fnu4HI GCNGC 1 cut(s) 145
FokI GGATG 2 cut(s) 698, 1150
Fsp4HI GCNGC 1 cut(s) 145
FspBI CTAG 6 cut(s) 101, 419, 461, 884, 1091, 1358
GlaI GCGC 1 cut(s) 459
GluI GCNGC 1 cut(s) 145
GsaI CCCAGC 1 cut(s) 785
GsuI CTGGAG 1 cut(s) 399
HaeII RGCGCY 1 cut(s) 461
HaeIII GGCC 6 cut(s) 144, 153, 658, 972, 995, 1077
HapII CCGG 2 cut(s) 190, 202
HhaI GCGC 1 cut(s) 460
Hin1II CATG 6 cut(s) 221, 712, 771, 981, 1040, 1069
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 4 cut(s) 162, 241, 750, 1226
HpaII CCGG 2 cut(s) 190, 202
HphI GGTGA 5 cut(s) 190, 359, 548, 701, 1401
Hpy166II GTNNAC 1 cut(s) 873
Hpy188I TCNGA 8 cut(s) 319, 442, 505, 522, 606, 639, 1207, 1440
Hpy188III TCNNGA 3 cut(s) 245, 1278, 1398
Hpy8I GTNNAC 1 cut(s) 873
HpyAV CCTTC 8 cut(s) 517, 551, 557, 615, 620, 1020, 1072, 1416
HpyCH4III ACNGT 3 cut(s) 268, 943, 1177
HpyCH4IV ACGT 2 cut(s) 848, 1104
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 150
HpyF3I CTNAG 3 cut(s) 609, 786, 1313
HpySE526I ACGT 2 cut(s) 848, 1104
Hsp92II CATG 6 cut(s) 221, 712, 771, 981, 1040, 1069
HspAI GCGC 1 cut(s) 458
KpnI GGTACC 2 cut(s) 272, 780
Kzo9I GATC 6 cut(s) 5, 278, 304, 560, 1280, 1400
LmnI GCTCC 5 cut(s) 40, 216, 380, 426, 935
LweI GCATC 3 cut(s) 419, 1198, 1283
MaeI CTAG 6 cut(s) 101, 419, 461, 884, 1091, 1358
MaeII ACGT 2 cut(s) 848, 1104
MaeIII GTNAC 6 cut(s) 713, 820, 844, 1039, 1052, 1227
MalI GATC 6 cut(s) 7, 280, 306, 562, 1282, 1402
MboI GATC 6 cut(s) 5, 278, 304, 560, 1280, 1400
MboII GAAGA 9 cut(s) 34, 314, 407, 659, 752, 848, 961, 1033, 1133
MflI RGATCY 1 cut(s) 304
MluCI AATT 8 cut(s) 342, 516, 905, 959, 1154, 1234, 1294, 1392
MlyI GAGTC 2 cut(s) 744, 1235
MmeI TCCRAC 2 cut(s) 913, 1407
MseI TTAA 1 cut(s) 1017
MslI CAYNNNNRTG 3 cut(s) 878, 944, 982
MspA1I CMGCKG 1 cut(s) 785
MspI CCGG 2 cut(s) 190, 202
MspR9I CCNGG 7 cut(s) 190, 191, 202, 203, 801, 997, 1347
MvaI CCWGG 3 cut(s) 801, 997, 1347
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 2 cut(s) 101, 150
NciI CCSGG 4 cut(s) 190, 191, 202, 203
NdeII GATC 6 cut(s) 5, 278, 304, 560, 1280, 1400
NlaIII CATG 6 cut(s) 221, 712, 771, 981, 1040, 1069
NlaIV GGNNCC 4 cut(s) 206, 270, 778, 937
NmuCI GTSAC 4 cut(s) 820, 844, 1039, 1227
NspI RCATGY 2 cut(s) 712, 981
NspV TTCGAA 1 cut(s) 1420
OliI CACNNNNGTG 1 cut(s) 944
PciI ACATGT 1 cut(s) 977
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 171
PfeI GAWTC 2 cut(s) 162, 241
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 1 cut(s) 146
PleI GAGTC 2 cut(s) 744, 1234
PpsI GAGTC 2 cut(s) 744, 1234
PpuMI RGGWCCY 1 cut(s) 205
PscI ACATGT 1 cut(s) 977
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 3 cut(s) 799, 995, 1345
PspFI CCCAGC 1 cut(s) 781
PspGI CCWGG 3 cut(s) 799, 995, 1345
PspN4I GGNNCC 4 cut(s) 206, 270, 778, 937
PspPI GGNCC 5 cut(s) 171, 205, 656, 999, 1075
PspPPI RGGWCCY 1 cut(s) 205
PsuI RGATCY 1 cut(s) 304
PvuII CAGCTG 1 cut(s) 785
RsaI GTAC 4 cut(s) 270, 586, 778, 872
RsaNI GTAC 4 cut(s) 269, 585, 777, 871
RseI CAYNNNNRTG 3 cut(s) 878, 944, 982
SaqAI TTAA 1 cut(s) 1017
SatI GCNGC 1 cut(s) 145
Sau3AI GATC 6 cut(s) 5, 278, 304, 560, 1280, 1400
Sau96I GGNCC 5 cut(s) 171, 205, 656, 999, 1075
SchI GAGTC 2 cut(s) 744, 1235
ScrFI CCNGG 7 cut(s) 190, 191, 202, 203, 801, 997, 1347
SfaNI GCATC 3 cut(s) 419, 1198, 1283
SfcI CTRYAG 1 cut(s) 1373
SfiI GGCCNNNNNGGCC 1 cut(s) 150
SfuI TTCGAA 1 cut(s) 1420
SinI GGWCC 3 cut(s) 171, 205, 999
SmaI CCCGGG 2 cut(s) 191, 203
SmiMI CAYNNNNRTG 3 cut(s) 878, 944, 982
Sse9I AATT 8 cut(s) 342, 516, 905, 959, 1154, 1234, 1294, 1392
SsiI CCGC 4 cut(s) 38, 83, 145, 1132
SspMI CTAG 6 cut(s) 101, 419, 461, 884, 1091, 1358
StyD4I CCNGG 7 cut(s) 188, 189, 200, 201, 799, 995, 1345
StyI CCWWGG 1 cut(s) 967
TaaI ACNGT 3 cut(s) 268, 943, 1177
TaiI ACGT 2 cut(s) 851, 1107
TaqI TCGA 4 cut(s) 165, 281, 982, 1420
TaqII GACCGA 3 cut(s) 188, 1058, 1318
TasI AATT 8 cut(s) 342, 516, 905, 959, 1154, 1234, 1294, 1392
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 2 cut(s) 162, 241
Tru1I TTAA 1 cut(s) 1017
Tru9I TTAA 1 cut(s) 1017
TscAI CASTG 2 cut(s) 230, 1192
TseFI GTSAC 4 cut(s) 820, 844, 1039, 1227
Tsp45I GTSAC 4 cut(s) 820, 844, 1039, 1227
TspDTI ATGAA 3 cut(s) 281, 756, 1054
TspGWI ACGGA 2 cut(s) 1099, 1310
TspMI CCCGGG 2 cut(s) 189, 201
TspRI CASTG 2 cut(s) 230, 1192
Van91I CCANNNNNTGG 1 cut(s) 404
VpaK11BI GGWCC 3 cut(s) 171, 205, 999
XapI RAATTY 4 cut(s) 516, 959, 1294, 1392
XceI RCATGY 2 cut(s) 712, 981
XmaI CCCGGG 2 cut(s) 189, 201
XspI CTAG 6 cut(s) 101, 419, 461, 884, 1091, 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.