Rh6AG023500
ERF Family

Belongs to the methyltransferase superfamily. METTL16 RlmF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0009335
Physical Location & Seq
Forward (+)
2755604 .. 2758504
2901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG023500.1

Sequence Viewer

Length: 1272 bp
ATGGGATCGAATAAGAACAAGAAGAGGAGGAGGACAGCGGAGCAACCCACGACCCACCCGAGAAACAAATACTCCGATAACCCGCCCGACTTCACCGAACTAGCTTCTCTCTACCCGTCTTTCAAACCCTTCGTTTTCTTTGGCCGCGATGGCCGACCCAGAATCGACTGGACCAACTTCAATGCCACCCGGGAACTCACCCGGGTCCTGCTCCTCCACGACCACCGCCTCAACTGGTGGATTCCGGATGCTCAGCTATGCCCTACGGTACCCAATAGATCGAACTACATTCATTGGATTGAAGATCTTCTGTCATGTGAGATTATTGCCAAGACTACAAAAAATGGTGATAAAGTGAGAGGGTTTGATATAGGAACTGGAGCAAACTGCATATATCCACTTCTTGGTGCATCACTTCTGGGATGGAGCTTTGTTGGGTCAGATATGACTGATGTAGCGCTAGAGTGGGCTGAAAAGAATGTAAGAGATAATCCACATATCTCGGAACTTATTGAAATTAGAAAGGTAGAAGGTGGTGAAAACACCCTTCCAGCAAAAGGATCAAATAATGAGGCATTGGTTAGTACCAAAGGTGAAATAGATCTGACTGAGGATATGGCAAGGGAAGGGAATGCTCGTCTGAATACAGGGTATTATGGGCCACCTATCCTTCTTGGAGTGGTCAGGGATGGTGAGGAGTTCGACTTCTGCATGTGTAACCCTCCATTTTTTGAGACAATGGAAGAAGCAGGACTCAATCCAAAAACTTCATGTGGTGGTACCCCAGCTGAGATGATTTGTCCTGGTGGGGAGAAGGCATTTGTTACTCTCATTATAGAAGATAGTGTCAAGTTGAGGCATACTTTTCGGTGGTACACATCAATGCTAGGGAGGAAATCAAATCTCAAACTACTAACTTCAAAGCTTTGGGAAGTTGGAGCCACAGTAGTAAAGACAACTGAATTTGTCCAAGGCCAAACATGTCGATGGGGGTTGGCCTGGTCCTTTTTACCTCCTGTTAAGAAGATATTATCATCTCATGTGACCGAAAAGAGTAACCTCTCCTTCATGCTTGAGGGCCTTGAACGAAAACTAGGTGCCATACATGTCTTGCAGTCAGTTGAAGCCTATTTCCGCAATGCTGGTCTTTCAGCAAACCCCTGGCACACTTCTGGTGAGAGGCTCACTACAGCAGAGAGATGGTCAAGTTTCAGGATCATTCTCACCGATATTTCGAAGGTTGGAGGAAGTTCTGAAACAGAAGTTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000154 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008174 GO:0008649 GO:0008757 GO:0008988 GO:0009056 GO:0009057 GO:0009451 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016422 GO:0016433 GO:0016556 GO:0016740 GO:0016741 GO:0017069 GO:0017070 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0030629 GO:0031167 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032259 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0035613 GO:0040031 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043484 GO:0043487 GO:0043488 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0045935 GO:0046483 GO:0046700 GO:0048024 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050684 GO:0050779 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0052907 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0065007 GO:0065008 GO:0070475 GO:0071704 GO:0071840 GO:0080009 GO:0080090 GO:0090304 GO:0097159 GO:0120048 GO:0120049 GO:0140098 GO:0140102 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903311 GO:1903313 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_10

Protein Analysis

423

Amino Acids

47.46

Weight (kDa)

6.77

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_10 PF05971 14 - 185 1.2e-48 RNA methyltransferase
Methyltransf_10 PF05971 223 - 337 9.3e-27 RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 268, 779
AccB1I GGYRCC 3 cut(s) 268, 779, 1097
AccB7I CCANNNNNTGG 1 cut(s) 404
AccII CGCG 1 cut(s) 147
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 5 cut(s) 38, 83, 145, 226, 1135
AclWI GGATC 3 cut(s) 13, 568, 1223
AcoI YGGCCR 2 cut(s) 142, 151
AcsI RAATTY 1 cut(s) 962
AfaI GTAC 4 cut(s) 270, 586, 781, 875
AfeI AGCGCT 1 cut(s) 459
AfiI CCNNNNNNNGG 2 cut(s) 404, 557
AflIII ACRYGT 2 cut(s) 980, 1105
AgsI TTSAA 7 cut(s) 124, 181, 302, 515, 921, 1085, 1124
AjnI CCWGG 3 cut(s) 802, 998, 1160
AluBI AGCT 5 cut(s) 104, 256, 429, 788, 925
AluI AGCT 5 cut(s) 104, 256, 429, 788, 925
Alw26I GTCTC 1 cut(s) 728
AlwI GGATC 3 cut(s) 13, 568, 1223
Ama87I CYCGRG 3 cut(s) 58, 189, 201
Aor13HI TCCGGA 1 cut(s) 244
Aor51HI AGCGCT 1 cut(s) 459
AoxI GGCC 6 cut(s) 142, 151, 659, 973, 996, 1078
ApoI RAATTY 1 cut(s) 962
Asp700I GAANNNNTTC 1 cut(s) 306
Asp718I GGTACC 2 cut(s) 268, 779
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
AsuC2I CCSGG 4 cut(s) 190, 191, 202, 203
AsuHPI GGTGA 8 cut(s) 85, 190, 359, 548, 605, 704, 1187, 1216
AsuII TTCGAA 1 cut(s) 1235
AvaI CYCGRG 3 cut(s) 58, 189, 201
AvaII GGWCC 3 cut(s) 171, 205, 1002
BanI GGYRCC 3 cut(s) 268, 779, 1097
BccI CCATC 5 cut(s) 143, 417, 683, 981, 1194
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 3 cut(s) 804, 1000, 1162
BcnI CCSGG 4 cut(s) 190, 191, 202, 203
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 4 cut(s) 101, 461, 887, 1094
BfmI CTRYAG 1 cut(s) 1188
BfoI RGCGCY 1 cut(s) 461
BglI GCCNNNNNGGC 1 cut(s) 150
BglII AGATCT 2 cut(s) 304, 601
BisI GCNGC 1 cut(s) 145
BlpI GCTNAGC 1 cut(s) 252
BlsI GCNGC 1 cut(s) 146
Bme1390I CCNGG 7 cut(s) 190, 191, 202, 203, 804, 1000, 1162
Bme18I GGWCC 3 cut(s) 171, 205, 1002
BmeT110I CYCGRG 3 cut(s) 58, 189, 201
BmgT120I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
BmiI GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
BmrFI CCNGG 7 cut(s) 190, 191, 202, 203, 804, 1000, 1162
BmsI GCATC 2 cut(s) 238, 419
BpmI CTGGAG 1 cut(s) 399
Bpu1102I GCTNAGC 1 cut(s) 252
Bpu14I TTCGAA 1 cut(s) 1235
BpuEI CTTGAG 1 cut(s) 1094
BpuMI CCSGG 4 cut(s) 190, 191, 202, 203
BsaJI CCNNGG 4 cut(s) 189, 201, 970, 1160
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 930, 960
Bsc4I CCNNNNNNNGG 2 cut(s) 404, 557
Bse1I ACTGG 3 cut(s) 173, 239, 382
Bse3DI GCAATG 1 cut(s) 1144
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 3 cut(s) 804, 1000, 1162
BseDI CCNNGG 4 cut(s) 189, 201, 970, 1160
BseGI GGATG 3 cut(s) 253, 428, 694
BseLI CCNNNNNNNGG 2 cut(s) 404, 557
BseMI GCAATG 1 cut(s) 1144
BseMII CTCAG 3 cut(s) 266, 600, 780
BseNI ACTGG 3 cut(s) 173, 239, 382
BseRI GAGGAG 4 cut(s) 40, 43, 203, 710
BseYI CCCAGC 1 cut(s) 784
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 6 cut(s) 144, 153, 661, 975, 998, 1080
BshNI GGYRCC 3 cut(s) 268, 779, 1097
BsiHKCI CYCGRG 3 cut(s) 58, 189, 201
BsiSI CCGG 3 cut(s) 190, 202, 245
BslI CCNNNNNNNGG 2 cut(s) 404, 557
BsmAI GTCTC 1 cut(s) 728
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 6 cut(s) 144, 153, 661, 975, 998, 1080
BsoBI CYCGRG 3 cut(s) 58, 189, 201
Bsp119I TTCGAA 1 cut(s) 1235
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 6 cut(s) 5, 278, 304, 560, 601, 1215
Bsp1720I GCTNAGC 1 cut(s) 252
BspACI CCGC 5 cut(s) 38, 83, 145, 226, 1135
BspANI GGCC 6 cut(s) 144, 153, 661, 975, 998, 1080
BspCNI CTCAG 3 cut(s) 265, 601, 781
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
BspPI GGATC 3 cut(s) 13, 568, 1223
BspT104I TTCGAA 1 cut(s) 1235
BspT107I GGYRCC 3 cut(s) 268, 779, 1097
BsrDI GCAATG 1 cut(s) 1144
BsrI ACTGG 3 cut(s) 173, 239, 382
BssECI CCNNGG 4 cut(s) 189, 201, 970, 1160
BssMI GATC 6 cut(s) 5, 278, 304, 560, 601, 1215
BssT1I CCWWGG 1 cut(s) 970
Bst2UI CCWGG 3 cut(s) 804, 1000, 1162
Bst4CI ACNGT 2 cut(s) 268, 946
Bst6I CTCTTC 1 cut(s) 17
BstBI TTCGAA 1 cut(s) 1235
BstDEI CTNAG 3 cut(s) 252, 609, 789
BstF5I GGATG 3 cut(s) 253, 428, 694
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 461
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 6 cut(s) 8, 281, 307, 563, 604, 1218
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 6 cut(s) 5, 278, 304, 560, 601, 1215
BstMWI GCNNNNNNNGC 1 cut(s) 150
BstNI CCWGG 3 cut(s) 804, 1000, 1162
BstNSI RCATGY 3 cut(s) 715, 984, 1109
BstSCI CCNGG 7 cut(s) 188, 189, 200, 201, 802, 998, 1160
BstSFI CTRYAG 1 cut(s) 1188
BstUI CGCG 1 cut(s) 147
BstX2I RGATCY 2 cut(s) 304, 601
BstYI RGATCY 2 cut(s) 304, 601
BsuRI GGCC 6 cut(s) 144, 153, 661, 975, 998, 1080
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 3 cut(s) 253, 428, 694
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
Cfr9I CCCGGG 2 cut(s) 189, 201
Csp6I GTAC 4 cut(s) 269, 585, 780, 874
CviAII CATG 7 cut(s) 315, 712, 771, 981, 1040, 1069, 1106
CviQI GTAC 4 cut(s) 269, 585, 780, 874
DdeI CTNAG 3 cut(s) 252, 609, 789
DpnI GATC 6 cut(s) 7, 280, 306, 562, 603, 1217
DpnII GATC 6 cut(s) 5, 278, 304, 560, 601, 1215
EaeI YGGCCR 2 cut(s) 142, 151
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 970
Eco47I GGWCC 3 cut(s) 171, 205, 1002
Eco47III AGCGCT 1 cut(s) 459
Eco88I CYCGRG 3 cut(s) 58, 189, 201
EcoO109I RGGNCCY 2 cut(s) 205, 1078
EcoRII CCWGG 3 cut(s) 802, 998, 1160
EcoT14I CCWWGG 1 cut(s) 970
ErhI CCWWGG 1 cut(s) 970
FaeI CATG 7 cut(s) 318, 715, 774, 984, 1043, 1072, 1109
FatI CATG 7 cut(s) 314, 711, 770, 980, 1039, 1068, 1105
FauI CCCGC 1 cut(s) 90
Fnu4HI GCNGC 1 cut(s) 145
FokI GGATG 3 cut(s) 260, 435, 701
Fsp4HI GCNGC 1 cut(s) 145
FspBI CTAG 4 cut(s) 101, 461, 887, 1094
GlaI GCGC 1 cut(s) 459
GluI GCNGC 1 cut(s) 145
GsaI CCCAGC 1 cut(s) 788
GsuI CTGGAG 1 cut(s) 399
HaeII RGCGCY 1 cut(s) 461
HaeIII GGCC 6 cut(s) 144, 153, 661, 975, 998, 1080
HapII CCGG 3 cut(s) 190, 202, 245
HhaI GCGC 1 cut(s) 460
Hin1II CATG 7 cut(s) 318, 715, 774, 984, 1043, 1072, 1109
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 3 cut(s) 162, 241, 753
HpaII CCGG 3 cut(s) 190, 202, 245
HphI GGTGA 8 cut(s) 85, 190, 359, 548, 605, 704, 1187, 1216
Hpy166II GTNNAC 1 cut(s) 876
Hpy188I TCNGA 6 cut(s) 76, 442, 505, 606, 642, 1255
Hpy188III TCNNGA 2 cut(s) 245, 1213
Hpy8I GTNNAC 1 cut(s) 876
HpyAV CCTTC 8 cut(s) 139, 524, 557, 620, 680, 808, 1075, 1231
HpyCH4III ACNGT 2 cut(s) 268, 946
HpyCH4V TGCA 4 cut(s) 390, 410, 711, 1114
HpyF10VI GCNNNNNNNGC 1 cut(s) 150
HpyF3I CTNAG 3 cut(s) 252, 609, 789
Hsp92II CATG 7 cut(s) 318, 715, 774, 984, 1043, 1072, 1109
HspAI GCGC 1 cut(s) 458
Kpn2I TCCGGA 1 cut(s) 244
KpnI GGTACC 2 cut(s) 272, 783
Kzo9I GATC 6 cut(s) 5, 278, 304, 560, 601, 1215
LmnI GCTCC 5 cut(s) 40, 216, 380, 426, 938
LweI GCATC 2 cut(s) 238, 419
MaeI CTAG 4 cut(s) 101, 461, 887, 1094
MaeIII GTNAC 4 cut(s) 716, 823, 1042, 1055
MalI GATC 6 cut(s) 7, 280, 306, 562, 603, 1217
MboI GATC 6 cut(s) 5, 278, 304, 560, 601, 1215
MboII GAAGA 6 cut(s) 34, 299, 314, 755, 851, 1036
MflI RGATCY 2 cut(s) 304, 601
MluCI AATT 2 cut(s) 516, 962
MlyI GAGTC 1 cut(s) 747
MmeI TCCRAC 2 cut(s) 916, 1222
MroI TCCGGA 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 1 cut(s) 1020
MslI CAYNNNNRTG 2 cut(s) 881, 985
MspA1I CMGCKG 2 cut(s) 38, 788
MspI CCGG 3 cut(s) 190, 202, 245
MspR9I CCNGG 7 cut(s) 190, 191, 202, 203, 804, 1000, 1162
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 3 cut(s) 804, 1000, 1162
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 1 cut(s) 150
NciI CCSGG 4 cut(s) 190, 191, 202, 203
NdeII GATC 6 cut(s) 5, 278, 304, 560, 601, 1215
NlaIII CATG 7 cut(s) 318, 715, 774, 984, 1043, 1072, 1109
NlaIV GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
NmuCI GTSAC 1 cut(s) 1042
NspI RCATGY 3 cut(s) 715, 984, 1109
NspV TTCGAA 1 cut(s) 1235
PciI ACATGT 2 cut(s) 980, 1105
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PctI GAATGC 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 2 cut(s) 162, 241
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 1 cut(s) 146
PleI GAGTC 1 cut(s) 747
PpsI GAGTC 1 cut(s) 747
PpuMI RGGWCCY 1 cut(s) 205
PscI ACATGT 2 cut(s) 980, 1105
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 3 cut(s) 802, 998, 1160
PspFI CCCAGC 1 cut(s) 784
PspGI CCWGG 3 cut(s) 802, 998, 1160
PspN4I GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
PspPI GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
PspPPI RGGWCCY 1 cut(s) 205
PsuI RGATCY 2 cut(s) 304, 601
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 4 cut(s) 270, 586, 781, 875
RsaNI GTAC 4 cut(s) 269, 585, 780, 874
RseI CAYNNNNRTG 2 cut(s) 881, 985
SaqAI TTAA 1 cut(s) 1020
SatI GCNGC 1 cut(s) 145
Sau3AI GATC 6 cut(s) 5, 278, 304, 560, 601, 1215
Sau96I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
SchI GAGTC 1 cut(s) 747
ScrFI CCNGG 7 cut(s) 190, 191, 202, 203, 804, 1000, 1162
SfaNI GCATC 2 cut(s) 238, 419
SfcI CTRYAG 1 cut(s) 1188
SfiI GGCCNNNNNGGCC 1 cut(s) 150
SfuI TTCGAA 1 cut(s) 1235
SinI GGWCC 3 cut(s) 171, 205, 1002
SmaI CCCGGG 2 cut(s) 191, 203
SmiMI CAYNNNNRTG 2 cut(s) 881, 985
SmlI CTYRAG 1 cut(s) 1073
SmoI CTYRAG 1 cut(s) 1073
Sse9I AATT 2 cut(s) 516, 962
SsiI CCGC 5 cut(s) 38, 83, 145, 226, 1135
SspMI CTAG 4 cut(s) 101, 461, 887, 1094
StyD4I CCNGG 7 cut(s) 188, 189, 200, 201, 802, 998, 1160
StyI CCWWGG 1 cut(s) 970
TaaI ACNGT 2 cut(s) 268, 946
TaqI TCGA 6 cut(s) 8, 165, 281, 702, 985, 1235
TaqII GACCGA 1 cut(s) 1061
TasI AATT 2 cut(s) 516, 962
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 2 cut(s) 162, 241
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TseFI GTSAC 1 cut(s) 1042
Tsp45I GTSAC 1 cut(s) 1042
TspDTI ATGAA 3 cut(s) 281, 759, 1057
TspMI CCCGGG 2 cut(s) 189, 201
Van91I CCANNNNNTGG 1 cut(s) 404
VpaK11BI GGWCC 3 cut(s) 171, 205, 1002
XapI RAATTY 1 cut(s) 962
XceI RCATGY 3 cut(s) 715, 984, 1109
XmaI CCCGGG 2 cut(s) 189, 201
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 4 cut(s) 101, 461, 887, 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.