RchiOBHm_Chr6g0244411
ERF Family

Belongs to the methyltransferase superfamily. METTL16 RlmF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009335
Physical Location & Seq
Forward (+)
489584 .. 493018
3435 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21905

Sequence Viewer

Length: 1470 bp
ATGGGGTCGAATAAGAATAAGAAGAGGAGGAGGACGGCAGAGCGACCCACGACCCACCCAAGAAACAAATACTCTGATAACCCGCCCGACTTCGCCGAACTAGCTTCTCTCTACCCGTCTTTCAAACCCTTCGTTTTCTTTGGCCGCGATGGCCGACCCAGAATCGACTGGACCGACTTCAACGCCACCCGGGAACTCACCCGGGTCCTGCTCCTCCACGACCACCGCCTCAACTGGTGGATTCCGGATGGTCAGCTATGTCCTACGGTACCCAATAGATCGAACTACATTCATTGGATTGAAGATCTTCTGTTGTCTGAAATTATTGCCAAGACTACAAAAAATGGTGATAAAGTGAGGGGGTTTGATATAGGAACTGGAGCAAACTGCATATATCCACTTCTTGGTGCATCTCTTCTGGGCTGGAGCTTTGTTGGGTCAGATATGACAGATGTAGCACTAGAGTGGGCAGAAAAGAATGTTAGAGATAATCCACATATCTCGGAACTTATTGAAATTAGAAAGGTAGAAGGTGGTGAAAACACCCTTCCAGCAAAAGGATCAAATAATGAGGCGTTGGTTAGTACCAAAAGCGAAATAGATCTGACTGAGGATATGGCAAGGGAAGGGAATGCTTGTCTGAATACAGGGTATTATGGGCCACCTATCCTTCTTGGAGTGGTCAGGGATGGTGAGGAGTTCGACTTCTGCATGTGTAACCCTCCATTTTTTGAGACAATGAAAGAAGCAGGACTCAATCCAAAAACTTCATGTGGTGGTACCGCAGCTGAGATGATTTGTCCTGGCGGGGAAAAGGCATTTGTCACTCGCATTATAGAAGATAGTGTCACGTTGAGGCATACTTTTCGGTGGTACACATCAATGCTAGGGAGGAAATCAAATCTCAAATTACTAACATCAAAGCTTTGGGAAGTTGGAGCCACCGTAGTAAAGACAACTGAATTTGTCCAAGGCCAAACAAGTCGATGGGGGTTGGCCTGGTCCTTTTTACCGCCTGTTAAGAAGATATTATCATCTCATGTGACCGAAAAGAGTAACCTTTCCTTCATGCTTGAGGGCCTTGAACGAAAACTAGGTGCCATACATGTCTTGCAGTCAGTTGAAGCCTATTTCCGCGATGCTGGTGCATTGTGTAGATTGAACACATCCTCATTTACAGTTGATATCACTGCAAAAGATGATAAGTCAGATGCAAACTTGAAAGGTGAGTCACAGAATTGTGATGGAGTTGCAAGTTGTAATGATGTGCGAGAGGCATCTGGATCAAGCTGTTTGAATTGTCTGTCAAATGATCTAAGTTTCCGTGTTTCGGTCTTTCAGCAAACCCCTGGCACACTTCTGGTGAAAGGCTCACTACAGCAGAAAGATGGTCAAGTTTCAGGATCATTGTCACCGATATTTCAAAGGTTAGAAGAAGTTCTGAAACAGAAGTTCTATGGAGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000154 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008174 GO:0008649 GO:0008757 GO:0008988 GO:0009056 GO:0009057 GO:0009451 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016422 GO:0016433 GO:0016556 GO:0016740 GO:0016741 GO:0017069 GO:0017070 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0030629 GO:0031167 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032259 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0035613 GO:0040031 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043484 GO:0043487 GO:0043488 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0045935 GO:0046483 GO:0046700 GO:0048024 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050684 GO:0050779 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0052907 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0065007 GO:0065008 GO:0070475 GO:0071704 GO:0071840 GO:0080009 GO:0080090 GO:0090304 GO:0097159 GO:0120048 GO:0120049 GO:0140098 GO:0140102 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903311 GO:1903313 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_10

Protein Analysis

489

Amino Acids

54.44

Weight (kDa)

6.73

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_10 PF05971 14 - 183 5.6e-50 RNA methyltransferase
Methyltransf_10 PF05971 223 - 337 1.4e-27 RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 268, 779
AccB1I GGYRCC 3 cut(s) 268, 779, 1097
AccB7I CCANNNNNTGG 1 cut(s) 404
AccII CGCG 2 cut(s) 147, 1137
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 7 cut(s) 83, 145, 226, 783, 807, 1013, 1135
AclWI GGATC 3 cut(s) 568, 1291, 1411
AcoI YGGCCR 2 cut(s) 142, 151
AcsI RAATTY 1 cut(s) 962
AfaI GTAC 4 cut(s) 270, 586, 781, 875
AfiI CCNNNNNNNGG 3 cut(s) 404, 557, 1330
AflIII ACRYGT 1 cut(s) 1105
AjnI CCWGG 3 cut(s) 802, 998, 1348
AleI CACNNNNGTG 1 cut(s) 463
AluBI AGCT 6 cut(s) 104, 256, 429, 788, 925, 1290
AluI AGCT 6 cut(s) 104, 256, 429, 788, 925, 1290
Alw26I GTCTC 1 cut(s) 728
AlwI GGATC 3 cut(s) 568, 1291, 1411
Ama87I CYCGRG 2 cut(s) 189, 201
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 6 cut(s) 142, 151, 659, 973, 996, 1078
ApeKI GCWGC 1 cut(s) 785
ApoI RAATTY 1 cut(s) 962
Asp700I GAANNNNTTC 2 cut(s) 306, 1437
Asp718I GGTACC 2 cut(s) 268, 779
AspS9I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
AsuC2I CCSGG 4 cut(s) 190, 191, 202, 203
AsuHPI GGTGA 7 cut(s) 190, 359, 548, 704, 1238, 1375, 1404
AvaI CYCGRG 2 cut(s) 189, 201
AvaII GGWCC 3 cut(s) 171, 205, 1002
BanI GGYRCC 3 cut(s) 268, 779, 1097
BbvI GCAGC 1 cut(s) 797
BccI CCATC 6 cut(s) 143, 242, 683, 981, 1238, 1382
BceAI ACGGC 1 cut(s) 51
BcgI CGANNNNNNTGC 4 cut(s) 848, 882, 1127, 1161
BciT130I CCWGG 3 cut(s) 804, 1000, 1350
BcnI CCSGG 4 cut(s) 190, 191, 202, 203
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 4 cut(s) 101, 461, 887, 1094
BfmI CTRYAG 1 cut(s) 1376
BglI GCCNNNNNGGC 1 cut(s) 150
BglII AGATCT 2 cut(s) 304, 601
BisI GCNGC 2 cut(s) 145, 786
BlsI GCNGC 2 cut(s) 146, 787
Bme1390I CCNGG 7 cut(s) 190, 191, 202, 203, 804, 1000, 1350
Bme18I GGWCC 3 cut(s) 171, 205, 1002
BmeT110I CYCGRG 2 cut(s) 189, 201
BmgT120I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
BmiI GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
BmrFI CCNGG 7 cut(s) 190, 191, 202, 203, 804, 1000, 1350
BmsI GCATC 4 cut(s) 419, 1129, 1201, 1286
BpmI CTGGAG 2 cut(s) 399, 445
BpuEI CTTGAG 1 cut(s) 1094
BpuMI CCSGG 4 cut(s) 190, 191, 202, 203
BsaJI CCNNGG 4 cut(s) 189, 201, 970, 1348
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bsc4I CCNNNNNNNGG 3 cut(s) 404, 557, 1330
Bse1I ACTGG 3 cut(s) 173, 239, 382
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 3 cut(s) 804, 1000, 1350
BseDI CCNNGG 4 cut(s) 189, 201, 970, 1348
BseGI GGATG 3 cut(s) 253, 694, 1166
BseLI CCNNNNNNNGG 3 cut(s) 404, 557, 1330
BseMII CTCAG 2 cut(s) 600, 780
BseNI ACTGG 3 cut(s) 173, 239, 382
BseRI GAGGAG 4 cut(s) 40, 43, 203, 710
BseXI GCAGC 1 cut(s) 797
Bsh1236I CGCG 2 cut(s) 147, 1137
BshFI GGCC 6 cut(s) 144, 153, 661, 975, 998, 1080
BshNI GGYRCC 3 cut(s) 268, 779, 1097
BsiHKCI CYCGRG 2 cut(s) 189, 201
BsiSI CCGG 3 cut(s) 190, 202, 245
BslI CCNNNNNNNGG 3 cut(s) 404, 557, 1330
BsmAI GTCTC 1 cut(s) 728
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 6 cut(s) 144, 153, 661, 975, 998, 1080
BsoBI CYCGRG 2 cut(s) 189, 201
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 7 cut(s) 278, 304, 560, 601, 1283, 1312, 1403
BspACI CCGC 7 cut(s) 83, 145, 226, 783, 807, 1013, 1135
BspANI GGCC 6 cut(s) 144, 153, 661, 975, 998, 1080
BspCNI CTCAG 2 cut(s) 601, 781
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 2 cut(s) 147, 1137
BspLI GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
BspPI GGATC 3 cut(s) 568, 1291, 1411
BspT107I GGYRCC 3 cut(s) 268, 779, 1097
BsrI ACTGG 3 cut(s) 173, 239, 382
BssECI CCNNGG 4 cut(s) 189, 201, 970, 1348
BssMI GATC 7 cut(s) 278, 304, 560, 601, 1283, 1312, 1403
BssT1I CCWWGG 1 cut(s) 970
Bst2UI CCWGG 3 cut(s) 804, 1000, 1350
Bst4CI ACNGT 3 cut(s) 268, 946, 1180
Bst6I CTCTTC 2 cut(s) 17, 420
BstDEI CTNAG 3 cut(s) 609, 789, 1316
BstF5I GGATG 3 cut(s) 253, 694, 1166
BstFNI CGCG 2 cut(s) 147, 1137
BstKTI GATC 7 cut(s) 281, 307, 563, 604, 1286, 1315, 1406
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 7 cut(s) 278, 304, 560, 601, 1283, 1312, 1403
BstMWI GCNNNNNNNGC 2 cut(s) 101, 150
BstNI CCWGG 3 cut(s) 804, 1000, 1350
BstNSI RCATGY 2 cut(s) 715, 1109
BstSCI CCNGG 7 cut(s) 188, 189, 200, 201, 802, 998, 1348
BstSFI CTRYAG 1 cut(s) 1376
BstUI CGCG 2 cut(s) 147, 1137
BstV1I GCAGC 1 cut(s) 797
BstX2I RGATCY 2 cut(s) 304, 601
BstYI RGATCY 2 cut(s) 304, 601
BsuRI GGCC 6 cut(s) 144, 153, 661, 975, 998, 1080
BtgZI GCGATG 2 cut(s) 162, 1152
BtsCI GGATG 3 cut(s) 253, 694, 1166
BtsI GCAGTG 1 cut(s) 1188
BtsIMutI CAGTG 1 cut(s) 1188
Cfr13I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
Cfr9I CCCGGG 2 cut(s) 189, 201
Csp6I GTAC 4 cut(s) 269, 585, 780, 874
CviAII CATG 5 cut(s) 712, 771, 1040, 1069, 1106
CviQI GTAC 4 cut(s) 269, 585, 780, 874
DdeI CTNAG 3 cut(s) 609, 789, 1316
DpnI GATC 7 cut(s) 280, 306, 562, 603, 1285, 1314, 1405
DpnII GATC 7 cut(s) 278, 304, 560, 601, 1283, 1312, 1403
EaeI YGGCCR 2 cut(s) 142, 151
Eam1104I CTCTTC 2 cut(s) 17, 420
EarI CTCTTC 2 cut(s) 17, 420
Eco130I CCWWGG 1 cut(s) 970
Eco32I GATATC 1 cut(s) 1186
Eco47I GGWCC 3 cut(s) 171, 205, 1002
Eco88I CYCGRG 2 cut(s) 189, 201
EcoO109I RGGNCCY 2 cut(s) 205, 1078
EcoRII CCWGG 3 cut(s) 802, 998, 1348
EcoRV GATATC 1 cut(s) 1186
EcoT14I CCWWGG 1 cut(s) 970
ErhI CCWWGG 1 cut(s) 970
FaeI CATG 5 cut(s) 715, 774, 1043, 1072, 1109
FatI CATG 5 cut(s) 711, 770, 1039, 1068, 1105
FauI CCCGC 2 cut(s) 90, 800
Fnu4HI GCNGC 2 cut(s) 145, 786
FokI GGATG 3 cut(s) 260, 701, 1153
Fsp4HI GCNGC 2 cut(s) 145, 786
FspBI CTAG 4 cut(s) 101, 461, 887, 1094
GluI GCNGC 2 cut(s) 145, 786
GsuI CTGGAG 2 cut(s) 399, 445
HaeIII GGCC 6 cut(s) 144, 153, 661, 975, 998, 1080
HapII CCGG 3 cut(s) 190, 202, 245
Hin1II CATG 5 cut(s) 715, 774, 1043, 1072, 1109
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 4 cut(s) 162, 241, 753, 1229
HpaII CCGG 3 cut(s) 190, 202, 245
HphI GGTGA 7 cut(s) 190, 359, 548, 704, 1238, 1375, 1404
Hpy166II GTNNAC 1 cut(s) 876
Hpy188I TCNGA 8 cut(s) 76, 319, 442, 505, 606, 642, 1210, 1443
Hpy188III TCNNGA 3 cut(s) 245, 1281, 1401
Hpy8I GTNNAC 1 cut(s) 876
HpyAV CCTTC 6 cut(s) 139, 524, 557, 620, 680, 1075
HpyCH4III ACNGT 3 cut(s) 268, 946, 1180
HpyCH4IV ACGT 1 cut(s) 851
HpyCH4V TGCA 8 cut(s) 390, 410, 711, 1114, 1148, 1193, 1214, 1253
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 150
HpyF3I CTNAG 3 cut(s) 609, 789, 1316
HpySE526I ACGT 1 cut(s) 851
Hsp92II CATG 5 cut(s) 715, 774, 1043, 1072, 1109
Kpn2I TCCGGA 1 cut(s) 244
KpnI GGTACC 2 cut(s) 272, 783
Kzo9I GATC 7 cut(s) 278, 304, 560, 601, 1283, 1312, 1403
LmnI GCTCC 4 cut(s) 216, 380, 426, 938
Lsp1109I GCAGC 1 cut(s) 797
LweI GCATC 4 cut(s) 419, 1129, 1201, 1286
MaeI CTAG 4 cut(s) 101, 461, 887, 1094
MaeII ACGT 1 cut(s) 851
MaeIII GTNAC 7 cut(s) 716, 823, 847, 1042, 1055, 1230, 1410
MalI GATC 7 cut(s) 280, 306, 562, 603, 1285, 1314, 1405
MboI GATC 7 cut(s) 278, 304, 560, 601, 1283, 1312, 1403
MboII GAAGA 7 cut(s) 34, 299, 314, 407, 851, 1036, 1445
MflI RGATCY 2 cut(s) 304, 601
MluCI AATT 6 cut(s) 321, 516, 908, 962, 1237, 1297
MlyI GAGTC 2 cut(s) 747, 1238
MmeI TCCRAC 1 cut(s) 916
MroI TCCGGA 1 cut(s) 244
MroXI GAANNNNTTC 2 cut(s) 306, 1437
MseI TTAA 1 cut(s) 1020
MslI CAYNNNNRTG 2 cut(s) 463, 881
MspA1I CMGCKG 1 cut(s) 788
MspI CCGG 3 cut(s) 190, 202, 245
MspR9I CCNGG 7 cut(s) 190, 191, 202, 203, 804, 1000, 1350
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 3 cut(s) 804, 1000, 1350
MvnI CGCG 2 cut(s) 147, 1137
MwoI GCNNNNNNNGC 2 cut(s) 101, 150
NciI CCSGG 4 cut(s) 190, 191, 202, 203
NdeII GATC 7 cut(s) 278, 304, 560, 601, 1283, 1312, 1403
NlaIII CATG 5 cut(s) 715, 774, 1043, 1072, 1109
NlaIV GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
NmuCI GTSAC 5 cut(s) 823, 847, 1042, 1230, 1410
NspI RCATGY 2 cut(s) 715, 1109
OliI CACNNNNGTG 1 cut(s) 463
PciI ACATGT 1 cut(s) 1105
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PctI GAATGC 1 cut(s) 637
PdmI GAANNNNTTC 2 cut(s) 306, 1437
PfeI GAWTC 2 cut(s) 162, 241
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 2 cut(s) 146, 787
PleI GAGTC 2 cut(s) 747, 1237
PpsI GAGTC 2 cut(s) 747, 1237
PpuMI RGGWCCY 1 cut(s) 205
PscI ACATGT 1 cut(s) 1105
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 3 cut(s) 802, 998, 1348
PspGI CCWGG 3 cut(s) 802, 998, 1348
PspN4I GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
PspPI GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
PspPPI RGGWCCY 1 cut(s) 205
PsuI RGATCY 2 cut(s) 304, 601
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 4 cut(s) 270, 586, 781, 875
RsaNI GTAC 4 cut(s) 269, 585, 780, 874
RseI CAYNNNNRTG 2 cut(s) 463, 881
SaqAI TTAA 1 cut(s) 1020
SatI GCNGC 2 cut(s) 145, 786
Sau3AI GATC 7 cut(s) 278, 304, 560, 601, 1283, 1312, 1403
Sau96I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
SchI GAGTC 2 cut(s) 747, 1238
ScrFI CCNGG 7 cut(s) 190, 191, 202, 203, 804, 1000, 1350
SfaNI GCATC 4 cut(s) 419, 1129, 1201, 1286
SfcI CTRYAG 1 cut(s) 1376
SfiI GGCCNNNNNGGCC 1 cut(s) 150
SinI GGWCC 3 cut(s) 171, 205, 1002
SmaI CCCGGG 2 cut(s) 191, 203
SmiMI CAYNNNNRTG 2 cut(s) 463, 881
SmlI CTYRAG 1 cut(s) 1073
SmoI CTYRAG 1 cut(s) 1073
Sse9I AATT 6 cut(s) 321, 516, 908, 962, 1237, 1297
SsiI CCGC 7 cut(s) 83, 145, 226, 783, 807, 1013, 1135
SspMI CTAG 4 cut(s) 101, 461, 887, 1094
StyD4I CCNGG 7 cut(s) 188, 189, 200, 201, 802, 998, 1348
StyI CCWWGG 1 cut(s) 970
TaaI ACNGT 3 cut(s) 268, 946, 1180
TaiI ACGT 1 cut(s) 854
TaqI TCGA 5 cut(s) 8, 165, 281, 702, 985
TaqII GACCGA 3 cut(s) 188, 1061, 1321
TasI AATT 6 cut(s) 321, 516, 908, 962, 1237, 1297
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 2 cut(s) 162, 241
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TscAI CASTG 1 cut(s) 1195
TseFI GTSAC 5 cut(s) 823, 847, 1042, 1230, 1410
TseI GCWGC 1 cut(s) 785
Tsp45I GTSAC 5 cut(s) 823, 847, 1042, 1230, 1410
TspDTI ATGAA 4 cut(s) 281, 755, 759, 1057
TspGWI ACGGA 1 cut(s) 1313
TspMI CCCGGG 2 cut(s) 189, 201
TspRI CASTG 1 cut(s) 1195
Van91I CCANNNNNTGG 1 cut(s) 404
VpaK11BI GGWCC 3 cut(s) 171, 205, 1002
XapI RAATTY 1 cut(s) 962
XceI RCATGY 2 cut(s) 715, 1109
XmaI CCCGGG 2 cut(s) 189, 201
XmnI GAANNNNTTC 2 cut(s) 306, 1437
XspI CTAG 4 cut(s) 101, 461, 887, 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.