Rh6CG016400
ERF Family

Belongs to the methyltransferase superfamily. METTL16 RlmF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009335
Physical Location & Seq
Forward (+)
1741317 .. 1744959
3643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG016400.1

Sequence Viewer

Length: 1455 bp
ATGGGTAAAAAGAGGAGGAGGACGGCGGAGCGACCCACGACCCACCCGAGAAACAAATACTCCGATAACCCACCCGACTTCGCCGAACTAGCTTCTCTCTACCCGTCTTTCAAACCCTTCGTTTTCTTTGGCCGCGATGGCCGACCCAGAATCGACTGGACCGACTTCAACGCCACCCGGGAACTCACTCGGGTCCTGCTCCTCCACGACCACCGCCTCAACTGGTGGATTCCGGATGGTCAGCTATGCCCTACGGTACCCAATAGATCCAACTACATTCATTGGATTGAAGATCTTCTGTCGTCTGACATTACTGCCAAAACTACAAAAAATGGTGATAAAGTGAGGGGGTTTGATATAGGAACTGGAGCAAACTGCATATATCCACTTCTCGGTGCATCTCTTCTGGGGTGGAGCTTTGTTGGGTCAGATATGACTGATGTAGCGCTAGAGTGGGCTGAAAAGAATGTCAGAGATAATCCACATATCTCAGAACTTATTGAAATTAGAAAGGTAGAAAGTGGTGAAAACACCCTTCCAGCAGAAGGATCAAACAATGAGGCATTGGTTAGTACCAAAGGCGAAATAGATCTGACTGAGGATATGGCAAGGGAAGGGAATAGTCTGAATACAGGGTATTATGGGCCACCTGTTCTTTTTGGAGTGGTCAGGGATGGTGAGGACTTCGACTTTTGCATGTGTAACCCTCCATTTTTTGAGACAATGGAAGAAGCAGGACTCAATCCAAAAACTTCATGCGGTGGTACCCCAGCTGAGATGATTTGTCCTGGTGGGGAGAAGGCATTTGTCACTCGCATTATAGAAGATAGTGTCAAGTTGAGGCATACTTTTCGGTGGTACACATCAATGCTAGGGAGGAAATCAAATCTCAAATTATTAACATCAAAGCTTTGGGAAGTTGGAGCCACCGTAGTAAAGACTACTGAATTTGTCCAAGGCCAAACATGTCGATGGGGGTTGGCCTGGTCCTTTTTACCTCTTGTTAAGAAGATATTATCATCTCATGTGACCGAAAAGAGTAACCTTTCCTTCATGCTTCAGGGCCTTGAACGAAAACTAGGTGCCATACATGTCTTGCAGTCAGTTGAAGCCTATTTCCGCGATGCTGGTGCATTGTGTAAATTGAACACATCCTCATTTACAGTTGATATCACTGCAAAAGATGATAAGTCAGATCCAAACTTGAAAAGTGAGTCACAGAATTGTGATGGAGTTGCAAGTTGTGATGATGTGCAAGAGGCATCTGGATCAAGCTGTTTGAATTTGCTGTCAAATAACCTAAGTTTCCGTGTTTCGGTCTTTCAGCAAACCCCTGGCACACTTCTGGTGAAAGGCTCACTACAGCAGAGAGATGGTCAAGTTTCAGGATCATTCTCACTGATATTTCGAAGGTTGGAGGAAGTTCTGAAACAGAAGTTCCGTAGAGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000154 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008174 GO:0008649 GO:0008757 GO:0008988 GO:0009056 GO:0009057 GO:0009451 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016422 GO:0016433 GO:0016556 GO:0016740 GO:0016741 GO:0017069 GO:0017070 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0030629 GO:0031167 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032259 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0035613 GO:0040031 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043484 GO:0043487 GO:0043488 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0045935 GO:0046483 GO:0046700 GO:0048024 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050684 GO:0050779 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0052907 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0065007 GO:0065008 GO:0070475 GO:0071704 GO:0071840 GO:0080009 GO:0080090 GO:0090304 GO:0097159 GO:0120048 GO:0120049 GO:0140098 GO:0140102 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903311 GO:1903313 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_10

Protein Analysis

484

Amino Acids

54.16

Weight (kDa)

6.49

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_10 PF05971 10 - 180 2.2e-50 RNA methyltransferase
Methyltransf_10 PF05971 220 - 332 5.3e-27 RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 256, 764
AccB1I GGYRCC 3 cut(s) 256, 764, 1082
AccII CGCG 2 cut(s) 135, 1122
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 5 cut(s) 26, 133, 214, 759, 1120
AclWI GGATC 5 cut(s) 261, 556, 1190, 1276, 1396
AcoI YGGCCR 2 cut(s) 130, 139
AcsI RAATTY 2 cut(s) 947, 1282
AcuI CTGAAG 1 cut(s) 1043
AfaI GTAC 4 cut(s) 258, 574, 766, 860
AfeI AGCGCT 1 cut(s) 447
AfiI CCNNNNNNNGG 3 cut(s) 392, 545, 1315
AflIII ACRYGT 2 cut(s) 965, 1090
AgsI TTSAA 9 cut(s) 112, 169, 290, 503, 1070, 1109, 1147, 1207, 1282
AjnI CCWGG 3 cut(s) 787, 983, 1333
AluBI AGCT 6 cut(s) 92, 244, 417, 773, 910, 1275
AluI AGCT 6 cut(s) 92, 244, 417, 773, 910, 1275
Alw26I GTCTC 1 cut(s) 713
AlwI GGATC 5 cut(s) 261, 556, 1190, 1276, 1396
Ama87I CYCGRG 3 cut(s) 46, 177, 189
Aor13HI TCCGGA 1 cut(s) 232
Aor51HI AGCGCT 1 cut(s) 447
AoxI GGCC 6 cut(s) 130, 139, 644, 958, 981, 1063
ApoI RAATTY 2 cut(s) 947, 1282
Asp700I GAANNNNTTC 1 cut(s) 294
Asp718I GGTACC 2 cut(s) 256, 764
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 5 cut(s) 159, 193, 644, 987, 1063
AsuC2I CCSGG 2 cut(s) 178, 179
AsuHPI GGTGA 4 cut(s) 347, 536, 689, 1360
AsuII TTCGAA 1 cut(s) 1408
AvaI CYCGRG 3 cut(s) 46, 177, 189
AvaII GGWCC 3 cut(s) 159, 193, 987
BanI GGYRCC 3 cut(s) 256, 764, 1082
BccI CCATC 6 cut(s) 131, 230, 668, 966, 1223, 1367
BceAI ACGGC 1 cut(s) 39
BcgI CGANNNNNNTGC 4 cut(s) 833, 867, 1112, 1146
BciT130I CCWGG 3 cut(s) 789, 985, 1335
BcnI CCSGG 2 cut(s) 178, 179
BcoDI GTCTC 1 cut(s) 713
BfaI CTAG 4 cut(s) 89, 449, 872, 1079
BfmI CTRYAG 1 cut(s) 1361
BfoI RGCGCY 1 cut(s) 449
BglI GCCNNNNNGGC 1 cut(s) 138
BglII AGATCT 2 cut(s) 292, 589
BisI GCNGC 1 cut(s) 133
BlsI GCNGC 1 cut(s) 134
Bme1390I CCNGG 5 cut(s) 178, 179, 789, 985, 1335
Bme18I GGWCC 3 cut(s) 159, 193, 987
BmeT110I CYCGRG 3 cut(s) 46, 177, 189
BmgT120I GGNCC 5 cut(s) 159, 193, 644, 987, 1063
BmiI GGNNCC 5 cut(s) 194, 258, 766, 925, 1084
BmrFI CCNGG 5 cut(s) 178, 179, 789, 985, 1335
BmsI GCATC 3 cut(s) 407, 1114, 1271
BpmI CTGGAG 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 1408
BpuMI CCSGG 2 cut(s) 178, 179
BsaJI CCNNGG 3 cut(s) 177, 955, 1333
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 915, 945
Bsc4I CCNNNNNNNGG 3 cut(s) 392, 545, 1315
Bse1I ACTGG 3 cut(s) 161, 227, 370
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 3 cut(s) 789, 985, 1335
BseDI CCNNGG 3 cut(s) 177, 955, 1333
BseGI GGATG 3 cut(s) 241, 679, 1151
BseLI CCNNNNNNNGG 3 cut(s) 392, 545, 1315
BseMII CTCAG 3 cut(s) 504, 588, 765
BseNI ACTGG 3 cut(s) 161, 227, 370
BseRI GAGGAG 3 cut(s) 28, 31, 191
BseYI CCCAGC 1 cut(s) 769
Bsh1236I CGCG 2 cut(s) 135, 1122
BshFI GGCC 6 cut(s) 132, 141, 646, 960, 983, 1065
BshNI GGYRCC 3 cut(s) 256, 764, 1082
BsiHKCI CYCGRG 3 cut(s) 46, 177, 189
BsiSI CCGG 2 cut(s) 178, 233
BslI CCNNNNNNNGG 3 cut(s) 392, 545, 1315
BsmAI GTCTC 1 cut(s) 713
BsnI GGCC 6 cut(s) 132, 141, 646, 960, 983, 1065
BsoBI CYCGRG 3 cut(s) 46, 177, 189
Bsp119I TTCGAA 1 cut(s) 1408
Bsp13I TCCGGA 1 cut(s) 232
Bsp143I GATC 7 cut(s) 266, 292, 548, 589, 1195, 1268, 1388
BspACI CCGC 5 cut(s) 26, 133, 214, 759, 1120
BspANI GGCC 6 cut(s) 132, 141, 646, 960, 983, 1065
BspCNI CTCAG 3 cut(s) 503, 589, 766
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 2 cut(s) 135, 1122
BspLI GGNNCC 5 cut(s) 194, 258, 766, 925, 1084
BspPI GGATC 5 cut(s) 261, 556, 1190, 1276, 1396
BspT104I TTCGAA 1 cut(s) 1408
BspT107I GGYRCC 3 cut(s) 256, 764, 1082
BsrI ACTGG 3 cut(s) 161, 227, 370
BssECI CCNNGG 3 cut(s) 177, 955, 1333
BssMI GATC 7 cut(s) 266, 292, 548, 589, 1195, 1268, 1388
BssT1I CCWWGG 1 cut(s) 955
Bst2UI CCWGG 3 cut(s) 789, 985, 1335
Bst4CI ACNGT 3 cut(s) 256, 931, 1165
Bst6I CTCTTC 1 cut(s) 408
BstBI TTCGAA 1 cut(s) 1408
BstDEI CTNAG 4 cut(s) 490, 597, 774, 1301
BstF5I GGATG 3 cut(s) 241, 679, 1151
BstFNI CGCG 2 cut(s) 135, 1122
BstH2I RGCGCY 1 cut(s) 449
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 7 cut(s) 269, 295, 551, 592, 1198, 1271, 1391
BstMAI GTCTC 1 cut(s) 713
BstMBI GATC 7 cut(s) 266, 292, 548, 589, 1195, 1268, 1388
BstMWI GCNNNNNNNGC 2 cut(s) 89, 138
BstNI CCWGG 3 cut(s) 789, 985, 1335
BstNSI RCATGY 3 cut(s) 700, 969, 1094
BstSCI CCNGG 5 cut(s) 176, 177, 787, 983, 1333
BstSFI CTRYAG 1 cut(s) 1361
BstUI CGCG 2 cut(s) 135, 1122
BstX2I RGATCY 4 cut(s) 266, 292, 589, 1195
BstYI RGATCY 4 cut(s) 266, 292, 589, 1195
BsuRI GGCC 6 cut(s) 132, 141, 646, 960, 983, 1065
BtgZI GCGATG 2 cut(s) 150, 1137
BtsCI GGATG 3 cut(s) 241, 679, 1151
BtsI GCAGTG 1 cut(s) 1173
BtsIMutI CAGTG 2 cut(s) 1173, 1397
CfoI GCGC 1 cut(s) 448
Cfr13I GGNCC 5 cut(s) 159, 193, 644, 987, 1063
Cfr9I CCCGGG 1 cut(s) 177
Csp6I GTAC 4 cut(s) 257, 573, 765, 859
CviAII CATG 6 cut(s) 697, 756, 966, 1025, 1054, 1091
CviQI GTAC 4 cut(s) 257, 573, 765, 859
DdeI CTNAG 4 cut(s) 490, 597, 774, 1301
DpnI GATC 7 cut(s) 268, 294, 550, 591, 1197, 1270, 1390
DpnII GATC 7 cut(s) 266, 292, 548, 589, 1195, 1268, 1388
EaeI YGGCCR 2 cut(s) 130, 139
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
EciI GGCGGA 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 955
Eco32I GATATC 1 cut(s) 1171
Eco47I GGWCC 3 cut(s) 159, 193, 987
Eco47III AGCGCT 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 1043
Eco88I CYCGRG 3 cut(s) 46, 177, 189
EcoO109I RGGNCCY 2 cut(s) 193, 1063
EcoRII CCWGG 3 cut(s) 787, 983, 1333
EcoRV GATATC 1 cut(s) 1171
EcoT14I CCWWGG 1 cut(s) 955
ErhI CCWWGG 1 cut(s) 955
FaeI CATG 6 cut(s) 700, 759, 969, 1028, 1057, 1094
FatI CATG 6 cut(s) 696, 755, 965, 1024, 1053, 1090
Fnu4HI GCNGC 1 cut(s) 133
FokI GGATG 3 cut(s) 248, 686, 1138
Fsp4HI GCNGC 1 cut(s) 133
FspBI CTAG 4 cut(s) 89, 449, 872, 1079
GlaI GCGC 1 cut(s) 447
GluI GCNGC 1 cut(s) 133
GsaI CCCAGC 1 cut(s) 773
GsuI CTGGAG 1 cut(s) 387
HaeII RGCGCY 1 cut(s) 449
HaeIII GGCC 6 cut(s) 132, 141, 646, 960, 983, 1065
HapII CCGG 2 cut(s) 178, 233
HhaI GCGC 1 cut(s) 448
Hin1II CATG 6 cut(s) 700, 759, 969, 1028, 1057, 1094
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 4 cut(s) 150, 229, 738, 1214
HpaII CCGG 2 cut(s) 178, 233
HphI GGTGA 4 cut(s) 347, 536, 689, 1360
Hpy166II GTNNAC 1 cut(s) 861
Hpy188I TCNGA 9 cut(s) 64, 307, 430, 473, 493, 594, 627, 1195, 1428
Hpy188III TCNNGA 3 cut(s) 233, 1266, 1386
Hpy8I GTNNAC 1 cut(s) 861
HpyAV CCTTC 7 cut(s) 127, 539, 545, 608, 793, 1060, 1404
HpyCH4III ACNGT 3 cut(s) 256, 931, 1165
HpyCH4V TGCA 8 cut(s) 378, 398, 696, 1099, 1133, 1178, 1238, 1255
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 138
HpyF3I CTNAG 4 cut(s) 490, 597, 774, 1301
Hsp92II CATG 6 cut(s) 700, 759, 969, 1028, 1057, 1094
HspAI GCGC 1 cut(s) 446
Kpn2I TCCGGA 1 cut(s) 232
KpnI GGTACC 2 cut(s) 260, 768
Kzo9I GATC 7 cut(s) 266, 292, 548, 589, 1195, 1268, 1388
LmnI GCTCC 5 cut(s) 28, 204, 368, 414, 923
LweI GCATC 3 cut(s) 407, 1114, 1271
MaeI CTAG 4 cut(s) 89, 449, 872, 1079
MaeIII GTNAC 5 cut(s) 701, 808, 1027, 1040, 1215
MalI GATC 7 cut(s) 268, 294, 550, 591, 1197, 1270, 1390
MboI GATC 7 cut(s) 266, 292, 548, 589, 1195, 1268, 1388
MboII GAAGA 6 cut(s) 287, 302, 395, 740, 836, 1021
MflI RGATCY 4 cut(s) 266, 292, 589, 1195
MluCI AATT 6 cut(s) 504, 893, 947, 1142, 1222, 1282
MlyI GAGTC 2 cut(s) 732, 1223
MmeI TCCRAC 3 cut(s) 294, 901, 1395
MroI TCCGGA 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 2 cut(s) 899, 1005
MslI CAYNNNNRTG 2 cut(s) 866, 970
MspA1I CMGCKG 1 cut(s) 773
MspI CCGG 2 cut(s) 178, 233
MspR9I CCNGG 5 cut(s) 178, 179, 789, 985, 1335
MvaI CCWGG 3 cut(s) 789, 985, 1335
MvnI CGCG 2 cut(s) 135, 1122
MwoI GCNNNNNNNGC 2 cut(s) 89, 138
NciI CCSGG 2 cut(s) 178, 179
NdeII GATC 7 cut(s) 266, 292, 548, 589, 1195, 1268, 1388
NlaIII CATG 6 cut(s) 700, 759, 969, 1028, 1057, 1094
NlaIV GGNNCC 5 cut(s) 194, 258, 766, 925, 1084
NmuCI GTSAC 3 cut(s) 808, 1027, 1215
NspI RCATGY 3 cut(s) 700, 969, 1094
NspV TTCGAA 1 cut(s) 1408
PciI ACATGT 2 cut(s) 965, 1090
PcsI WCGNNNNNNNCGW 2 cut(s) 44, 159
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 2 cut(s) 150, 229
PkrI GCNGC 1 cut(s) 134
PleI GAGTC 2 cut(s) 732, 1222
PpsI GAGTC 2 cut(s) 732, 1222
PpuMI RGGWCCY 1 cut(s) 193
PscI ACATGT 2 cut(s) 965, 1090
Psp5II RGGWCCY 1 cut(s) 193
Psp6I CCWGG 3 cut(s) 787, 983, 1333
PspFI CCCAGC 1 cut(s) 769
PspGI CCWGG 3 cut(s) 787, 983, 1333
PspN4I GGNNCC 5 cut(s) 194, 258, 766, 925, 1084
PspPI GGNCC 5 cut(s) 159, 193, 644, 987, 1063
PspPPI RGGWCCY 1 cut(s) 193
PsuI RGATCY 4 cut(s) 266, 292, 589, 1195
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 4 cut(s) 258, 574, 766, 860
RsaNI GTAC 4 cut(s) 257, 573, 765, 859
RseI CAYNNNNRTG 2 cut(s) 866, 970
SaqAI TTAA 2 cut(s) 899, 1005
SatI GCNGC 1 cut(s) 133
Sau3AI GATC 7 cut(s) 266, 292, 548, 589, 1195, 1268, 1388
Sau96I GGNCC 5 cut(s) 159, 193, 644, 987, 1063
SchI GAGTC 2 cut(s) 732, 1223
ScrFI CCNGG 5 cut(s) 178, 179, 789, 985, 1335
SfaNI GCATC 3 cut(s) 407, 1114, 1271
SfcI CTRYAG 1 cut(s) 1361
SfiI GGCCNNNNNGGCC 1 cut(s) 138
SfuI TTCGAA 1 cut(s) 1408
SinI GGWCC 3 cut(s) 159, 193, 987
SmaI CCCGGG 1 cut(s) 179
SmiMI CAYNNNNRTG 2 cut(s) 866, 970
Sse9I AATT 6 cut(s) 504, 893, 947, 1142, 1222, 1282
SsiI CCGC 5 cut(s) 26, 133, 214, 759, 1120
SspMI CTAG 4 cut(s) 89, 449, 872, 1079
StyD4I CCNGG 5 cut(s) 176, 177, 787, 983, 1333
StyI CCWWGG 1 cut(s) 955
TaaI ACNGT 3 cut(s) 256, 931, 1165
TaqI TCGA 4 cut(s) 153, 687, 970, 1408
TaqII GACCGA 3 cut(s) 176, 1046, 1306
TasI AATT 6 cut(s) 504, 893, 947, 1142, 1222, 1282
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 2 cut(s) 150, 229
Tru1I TTAA 2 cut(s) 899, 1005
Tru9I TTAA 2 cut(s) 899, 1005
TscAI CASTG 2 cut(s) 1180, 1404
TseFI GTSAC 3 cut(s) 808, 1027, 1215
Tsp45I GTSAC 3 cut(s) 808, 1027, 1215
TspDTI ATGAA 3 cut(s) 269, 744, 1042
TspGWI ACGGA 2 cut(s) 1298, 1430
TspMI CCCGGG 1 cut(s) 177
TspRI CASTG 2 cut(s) 1180, 1404
VpaK11BI GGWCC 3 cut(s) 159, 193, 987
XapI RAATTY 2 cut(s) 947, 1282
XceI RCATGY 3 cut(s) 700, 969, 1094
XmaI CCCGGG 1 cut(s) 177
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 4 cut(s) 89, 449, 872, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.