Rh6BG019200
ERF Family

Belongs to the methyltransferase superfamily. METTL16 RlmF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009335
Physical Location & Seq
Forward (+)
2927413 .. 2932472
5060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG019200.1

Sequence Viewer

Length: 1104 bp
ATGGGGTCGAATAAGAATAAGAAGAGGAGGAGGACGGCGGAGCGACCCACGACCCATCCGAGAAACAAATACTCCGATAACCCGCCCGACTTCGCCAAACTAGCTTCTCTCTACCCGACTTTCAAACCTTTCGTTTTCTTTGGCCGCGATGGCCGACCCAGAATCGACTGGACCGACTTCAACGCCACCCGGGAACTCACCCGGGTCCTGCTCCTCCACGACCACCGCCTCAACTGGTGGATTCCGGATGGTCAGCTATGCCCTACAGTACCCAATAGATCGAACTACATTCATTGGATTGATGATCTTCTGTCGTCTGATATTATTGCCAAGACTACAAAAAATGGTGATAAAGTGAGGGGGTTTGATATAGGAACTGGAGCAAACTGCATATATGCACTTCTTGGTGCATCTCTTCTGGGGTGGTGCTTTGTTGGGTCAGATATGACTGATGTAGCGCTAGAGTGGGCTGAAAAGAATGTCAGAGATAATCCACATATCTCGGAACTTATTGAAATTAGAAAGGTAGAAAGTGGTGAAAACACCCTTCCAACAAAAGGATCAAATAATGAGGCATTGGTCAGTACCAAAAACGAAATAGATCTGACTGAGGATATGGGAAGGGAAAGGAATAGTCTGAATACAGGGTATTATGGGCCACCTATTCTTCTTGGAGTGGTCAGGGATGGTGAGGAGTTCGACTTTTGCATGTGTAACCCTCCATTTTTTGAGACAATGGAAGAAGCAGGACTCAATCCAAAAACTTCATGTGGTGGTACCCCAGCTGAGATGATTTGTCCTGGTGGGGAAAAGGCATTTATCACTCGCATTATAGAAGATAGTGTCATGTTGAAGCATACTTTTCGGTGGTACACATCAATGCTAGGGAGGAAATCAAATCTCAAATTACTAACATCAAAGCTTTGGGAAGTTGGAGCCACCGTAGTAAAGACAACTGAATTTGTCCAAGGCCAGACATGTCGATGGGGGTTGGCCTGGTCCTTTTTACCTCCTGTTAAGAAGATATTATCATCACATGTGACCGAAAAGAGTAACCTCTCCTTCATGCTTGAGGTTTCGACGACTTTCTTTTATGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000154 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008174 GO:0008649 GO:0008757 GO:0008988 GO:0009056 GO:0009057 GO:0009451 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016422 GO:0016433 GO:0016556 GO:0016740 GO:0016741 GO:0017069 GO:0017070 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0030629 GO:0031167 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032259 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0035613 GO:0040031 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043484 GO:0043487 GO:0043488 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0045935 GO:0046483 GO:0046700 GO:0048024 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050684 GO:0050779 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0052907 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0065007 GO:0065008 GO:0070475 GO:0071704 GO:0071840 GO:0080009 GO:0080090 GO:0090304 GO:0097159 GO:0120048 GO:0120049 GO:0140098 GO:0140102 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903311 GO:1903313 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_10

Protein Analysis

367

Amino Acids

41.53

Weight (kDa)

7.58

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_10 PF05971 14 - 181 2.1e-48 RNA methyltransferase
Methyltransf_10 PF05971 222 - 336 9e-28 RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 776
AccB1I GGYRCC 1 cut(s) 776
AccII CGCG 1 cut(s) 147
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 4 cut(s) 38, 83, 145, 226
AclWI GGATC 1 cut(s) 568
AcoI YGGCCR 2 cut(s) 142, 151
AcsI RAATTY 1 cut(s) 959
AfaI GTAC 4 cut(s) 270, 586, 778, 872
AfeI AGCGCT 1 cut(s) 459
AfiI CCNNNNNNNGG 1 cut(s) 557
AflIII ACRYGT 2 cut(s) 977, 1036
AgsI TTSAA 4 cut(s) 124, 181, 515, 853
AjnI CCWGG 2 cut(s) 799, 995
AluBI AGCT 4 cut(s) 104, 256, 785, 922
AluI AGCT 4 cut(s) 104, 256, 785, 922
Alw26I GTCTC 1 cut(s) 725
AlwI GGATC 1 cut(s) 568
Ama87I CYCGRG 2 cut(s) 189, 201
Aor13HI TCCGGA 1 cut(s) 244
Aor51HI AGCGCT 1 cut(s) 459
AoxI GGCC 5 cut(s) 142, 151, 656, 970, 993
ApoI RAATTY 1 cut(s) 959
Asp718I GGTACC 1 cut(s) 776
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 4 cut(s) 171, 205, 656, 999
AsuC2I CCSGG 4 cut(s) 190, 191, 202, 203
AsuHPI GGTGA 4 cut(s) 190, 359, 548, 701
AvaI CYCGRG 2 cut(s) 189, 201
AvaII GGWCC 3 cut(s) 171, 205, 999
BanI GGYRCC 1 cut(s) 776
BccI CCATC 5 cut(s) 63, 143, 242, 680, 978
BceAI ACGGC 1 cut(s) 51
BcgI CGANNNNNNTGC 2 cut(s) 845, 879
BciT130I CCWGG 2 cut(s) 801, 997
BcnI CCSGG 4 cut(s) 190, 191, 202, 203
BcoDI GTCTC 1 cut(s) 725
BfaI CTAG 3 cut(s) 101, 461, 884
BfmI CTRYAG 1 cut(s) 264
BfoI RGCGCY 1 cut(s) 461
BglI GCCNNNNNGGC 1 cut(s) 150
BglII AGATCT 1 cut(s) 601
BisI GCNGC 1 cut(s) 145
BlsI GCNGC 1 cut(s) 146
Bme1390I CCNGG 6 cut(s) 190, 191, 202, 203, 801, 997
Bme18I GGWCC 3 cut(s) 171, 205, 999
BmeT110I CYCGRG 2 cut(s) 189, 201
BmgT120I GGNCC 4 cut(s) 171, 205, 656, 999
BmiI GGNNCC 3 cut(s) 206, 778, 937
BmrFI CCNGG 6 cut(s) 190, 191, 202, 203, 801, 997
BmsI GCATC 1 cut(s) 419
BpmI CTGGAG 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 1091
BpuMI CCSGG 4 cut(s) 190, 191, 202, 203
BsaJI CCNNGG 3 cut(s) 189, 201, 967
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 927, 957
Bsc4I CCNNNNNNNGG 1 cut(s) 557
Bse1I ACTGG 3 cut(s) 173, 239, 382
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 2 cut(s) 801, 997
BseDI CCNNGG 3 cut(s) 189, 201, 967
BseGI GGATG 3 cut(s) 55, 253, 691
BseLI CCNNNNNNNGG 1 cut(s) 557
BseMII CTCAG 2 cut(s) 600, 777
BseNI ACTGG 3 cut(s) 173, 239, 382
BseRI GAGGAG 4 cut(s) 40, 43, 203, 707
BseYI CCCAGC 1 cut(s) 781
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 5 cut(s) 144, 153, 658, 972, 995
BshNI GGYRCC 1 cut(s) 776
BsiHKCI CYCGRG 2 cut(s) 189, 201
BsiSI CCGG 3 cut(s) 190, 202, 245
BslI CCNNNNNNNGG 1 cut(s) 557
BsmAI GTCTC 1 cut(s) 725
BsnI GGCC 5 cut(s) 144, 153, 658, 972, 995
BsoBI CYCGRG 2 cut(s) 189, 201
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 4 cut(s) 278, 304, 560, 601
BspACI CCGC 4 cut(s) 38, 83, 145, 226
BspANI GGCC 5 cut(s) 144, 153, 658, 972, 995
BspCNI CTCAG 2 cut(s) 601, 778
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 3 cut(s) 206, 778, 937
BspPI GGATC 1 cut(s) 568
BspT107I GGYRCC 1 cut(s) 776
BsrI ACTGG 3 cut(s) 173, 239, 382
BssECI CCNNGG 3 cut(s) 189, 201, 967
BssMI GATC 4 cut(s) 278, 304, 560, 601
BssT1I CCWWGG 1 cut(s) 967
Bst2UI CCWGG 2 cut(s) 801, 997
Bst4CI ACNGT 2 cut(s) 268, 943
Bst6I CTCTTC 2 cut(s) 17, 420
BstDEI CTNAG 2 cut(s) 609, 786
BstF5I GGATG 3 cut(s) 55, 253, 691
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 461
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 4 cut(s) 281, 307, 563, 604
BstMAI GTCTC 1 cut(s) 725
BstMBI GATC 4 cut(s) 278, 304, 560, 601
BstMWI GCNNNNNNNGC 2 cut(s) 101, 150
BstNI CCWGG 2 cut(s) 801, 997
BstNSI RCATGY 3 cut(s) 712, 981, 1040
BstSCI CCNGG 6 cut(s) 188, 189, 200, 201, 799, 995
BstSFI CTRYAG 1 cut(s) 264
BstUI CGCG 1 cut(s) 147
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
BsuRI GGCC 5 cut(s) 144, 153, 658, 972, 995
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 3 cut(s) 55, 253, 691
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 4 cut(s) 171, 205, 656, 999
Cfr9I CCCGGG 2 cut(s) 189, 201
Csp6I GTAC 4 cut(s) 269, 585, 777, 871
CviAII CATG 6 cut(s) 709, 768, 847, 978, 1037, 1066
CviQI GTAC 4 cut(s) 269, 585, 777, 871
DdeI CTNAG 2 cut(s) 609, 786
DpnI GATC 4 cut(s) 280, 306, 562, 603
DpnII GATC 4 cut(s) 278, 304, 560, 601
EaeI YGGCCR 2 cut(s) 142, 151
Eam1104I CTCTTC 2 cut(s) 17, 420
EarI CTCTTC 2 cut(s) 17, 420
EciI GGCGGA 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 967
Eco47I GGWCC 3 cut(s) 171, 205, 999
Eco47III AGCGCT 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 189, 201
EcoO109I RGGNCCY 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 799, 995
EcoT14I CCWWGG 1 cut(s) 967
ErhI CCWWGG 1 cut(s) 967
FaeI CATG 6 cut(s) 712, 771, 850, 981, 1040, 1069
FatI CATG 6 cut(s) 708, 767, 846, 977, 1036, 1065
FauI CCCGC 1 cut(s) 90
Fnu4HI GCNGC 1 cut(s) 145
FokI GGATG 3 cut(s) 42, 260, 698
Fsp4HI GCNGC 1 cut(s) 145
FspBI CTAG 3 cut(s) 101, 461, 884
GlaI GCGC 1 cut(s) 459
GluI GCNGC 1 cut(s) 145
GsaI CCCAGC 1 cut(s) 785
GsuI CTGGAG 1 cut(s) 399
HaeII RGCGCY 1 cut(s) 461
HaeIII GGCC 5 cut(s) 144, 153, 658, 972, 995
HapII CCGG 3 cut(s) 190, 202, 245
HhaI GCGC 1 cut(s) 460
Hin1II CATG 6 cut(s) 712, 771, 850, 981, 1040, 1069
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 3 cut(s) 162, 241, 750
HpaII CCGG 3 cut(s) 190, 202, 245
HphI GGTGA 4 cut(s) 190, 359, 548, 701
Hpy166II GTNNAC 1 cut(s) 873
Hpy188I TCNGA 8 cut(s) 60, 76, 319, 442, 485, 505, 606, 639
Hpy188III TCNNGA 1 cut(s) 245
Hpy8I GTNNAC 1 cut(s) 873
Hpy99I CGWCG 1 cut(s) 1084
HpyAV CCTTC 3 cut(s) 557, 615, 1072
HpyCH4III ACNGT 2 cut(s) 268, 943
HpyCH4V TGCA 4 cut(s) 390, 398, 410, 708
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 150
HpyF3I CTNAG 2 cut(s) 609, 786
Hsp92II CATG 6 cut(s) 712, 771, 850, 981, 1040, 1069
HspAI GCGC 1 cut(s) 458
Kpn2I TCCGGA 1 cut(s) 244
KpnI GGTACC 1 cut(s) 780
Kzo9I GATC 4 cut(s) 278, 304, 560, 601
LmnI GCTCC 4 cut(s) 40, 216, 380, 935
LweI GCATC 1 cut(s) 419
MaeI CTAG 3 cut(s) 101, 461, 884
MaeIII GTNAC 3 cut(s) 713, 1039, 1052
MalI GATC 4 cut(s) 280, 306, 562, 603
MboI GATC 4 cut(s) 278, 304, 560, 601
MboII GAAGA 7 cut(s) 34, 299, 407, 659, 752, 848, 1033
MflI RGATCY 1 cut(s) 601
MluCI AATT 3 cut(s) 516, 905, 959
MlyI GAGTC 1 cut(s) 744
MmeI TCCRAC 2 cut(s) 575, 913
MroI TCCGGA 1 cut(s) 244
MseI TTAA 2 cut(s) 1017, 1102
MslI CAYNNNNRTG 2 cut(s) 878, 982
MspA1I CMGCKG 1 cut(s) 785
MspI CCGG 3 cut(s) 190, 202, 245
MspR9I CCNGG 6 cut(s) 190, 191, 202, 203, 801, 997
MvaI CCWGG 2 cut(s) 801, 997
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 2 cut(s) 101, 150
NciI CCSGG 4 cut(s) 190, 191, 202, 203
NdeII GATC 4 cut(s) 278, 304, 560, 601
NlaIII CATG 6 cut(s) 712, 771, 850, 981, 1040, 1069
NlaIV GGNNCC 3 cut(s) 206, 778, 937
NmuCI GTSAC 1 cut(s) 1039
NspI RCATGY 3 cut(s) 712, 981, 1040
PciI ACATGT 2 cut(s) 977, 1036
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 171
PfeI GAWTC 2 cut(s) 162, 241
PkrI GCNGC 1 cut(s) 146
PleI GAGTC 1 cut(s) 744
PpsI GAGTC 1 cut(s) 744
PpuMI RGGWCCY 1 cut(s) 205
PscI ACATGT 2 cut(s) 977, 1036
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 2 cut(s) 799, 995
PspFI CCCAGC 1 cut(s) 781
PspGI CCWGG 2 cut(s) 799, 995
PspN4I GGNNCC 3 cut(s) 206, 778, 937
PspPI GGNCC 4 cut(s) 171, 205, 656, 999
PspPPI RGGWCCY 1 cut(s) 205
PsuI RGATCY 1 cut(s) 601
PvuII CAGCTG 1 cut(s) 785
RsaI GTAC 4 cut(s) 270, 586, 778, 872
RsaNI GTAC 4 cut(s) 269, 585, 777, 871
RseI CAYNNNNRTG 2 cut(s) 878, 982
SaqAI TTAA 2 cut(s) 1017, 1102
SatI GCNGC 1 cut(s) 145
Sau3AI GATC 4 cut(s) 278, 304, 560, 601
Sau96I GGNCC 4 cut(s) 171, 205, 656, 999
SchI GAGTC 1 cut(s) 744
ScrFI CCNGG 6 cut(s) 190, 191, 202, 203, 801, 997
SfaNI GCATC 1 cut(s) 419
SfcI CTRYAG 1 cut(s) 264
SfiI GGCCNNNNNGGCC 1 cut(s) 150
SinI GGWCC 3 cut(s) 171, 205, 999
SmaI CCCGGG 2 cut(s) 191, 203
SmiMI CAYNNNNRTG 2 cut(s) 878, 982
SmlI CTYRAG 1 cut(s) 1070
SmoI CTYRAG 1 cut(s) 1070
Sse9I AATT 3 cut(s) 516, 905, 959
SsiI CCGC 4 cut(s) 38, 83, 145, 226
SspMI CTAG 3 cut(s) 101, 461, 884
StyD4I CCNGG 6 cut(s) 188, 189, 200, 201, 799, 995
StyI CCWWGG 1 cut(s) 967
TaaI ACNGT 2 cut(s) 268, 943
TaqI TCGA 6 cut(s) 8, 165, 281, 699, 982, 1079
TaqII GACCGA 2 cut(s) 188, 1058
TasI AATT 3 cut(s) 516, 905, 959
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 2 cut(s) 162, 241
Tru1I TTAA 2 cut(s) 1017, 1102
Tru9I TTAA 2 cut(s) 1017, 1102
TseFI GTSAC 1 cut(s) 1039
Tsp45I GTSAC 1 cut(s) 1039
TspDTI ATGAA 3 cut(s) 281, 756, 1054
TspMI CCCGGG 2 cut(s) 189, 201
VpaK11BI GGWCC 3 cut(s) 171, 205, 999
XapI RAATTY 1 cut(s) 959
XceI RCATGY 3 cut(s) 712, 981, 1040
XmaI CCCGGG 2 cut(s) 189, 201
XspI CTAG 3 cut(s) 101, 461, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.