Rmu_sc0000633.1_g000005
ERF Family

Belongs to the methyltransferase superfamily. METTL16 RlmF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000633.1
OG0009335
Physical Location & Seq
Forward (+)
12712 .. 15605
2894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000633.1_g000005.1.cds

Sequence Viewer

Length: 1470 bp
atgggatcgaataagaacaagaagaggaggaggacggtggagcgacccacgacccacccgagaaacaaatactccgataacccgcccgacttcgccgaactagcttctctctacccatctttcaaacccttcgttttctttggccgtgatggccgacccagaatcgactggaccgacttcaacgccacccgggaactcacccgggtcctgctcctccacgaccaccgcctcaactggtggattccggatggtcagctatgccctacggtacccaatagatcgaactacattcattggattgaagatcttctgtcatgtgacattattgccaagactacaaaaaatggtgataaagtgagagggtttgatataggaactggagcaaactgcatatatccacttcttggtgcatcacttctgggatggagctttgttgggtcagatatgactgatgtagcgctagagtgggctgaaaagaatgtaagagataatccacatatctcggaacttattgaaattagaaaggtagaaggtggtgaaaacacccttccagcaaaaggatcaaataatgaggcattggttagtaccaaaagcgaaatagatctgactgaggatatggcaagggaagggaatgctcgtctgaatacagggtattatgggccacctatccttcttggagtggccagggatggtgaggagttcgacttctgcatgtgtaaccctccattttttgagacaatggaagaagcaggactcaatccaaaaacgtcatgtggtggtaccccagctgagatgatttgtcctggtggggagaaggcatttgttactcgcattatagaagatagtgtcaagttgaggcatacttttcggtggtacacatcaatgctagggaggaaatcaaatctcaaactactaacttcaaagctttgggaagttggagccacagtagtaaagacaactgaatttgtccaaggccaaacatgtcgatgggggttggcctggtcctttttacctcctgttaagaagatattatcatcacatgtgaccgaaaagagtaacctgtccttcatgcttgagggccttgaacgaaaactaggtgccatacatgtcttgcagtcagttgaagcctatttccgcaatgctggtgcattgtgtaaattgaacacatcctcatttacagttgatatcactgcaaaagatgataagtcagatgcaaacttgaaaagtgagtcacaaaagtgtgatgaagttgcaagttgtgatgatgtgcaagaggcatctggatcaagctgtttgaatttgctgtcaaataacctaagtttccgtgtttcggtctttcagcaaacccctggcacacttctggtgaaaggctcactacagcagagagatggccaaatttcaggatcattctcaccgatatttcgaaggttagaggaagttctgaaacagaagttccatagagagaagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000154 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008173 GO:0008174 GO:0008649 GO:0008757 GO:0008988 GO:0009056 GO:0009057 GO:0009451 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016422 GO:0016433 GO:0016556 GO:0016740 GO:0016741 GO:0017069 GO:0017070 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0022613 GO:0030629 GO:0031167 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032259 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0035613 GO:0040031 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043484 GO:0043487 GO:0043488 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0045935 GO:0046483 GO:0046700 GO:0048024 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050684 GO:0050779 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0052907 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0065007 GO:0065008 GO:0070475 GO:0071704 GO:0071840 GO:0080009 GO:0080090 GO:0090304 GO:0097159 GO:0120048 GO:0120049 GO:0140098 GO:0140102 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903311 GO:1903313 GO:2000112 GO:2000113
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_10

Protein Analysis

489

Amino Acids

54.79

Weight (kDa)

7.52

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 268, 779
AccB1I GGYRCC 3 cut(s) 268, 779, 1097
AccB7I CCANNNNNTGG 1 cut(s) 404
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 3 cut(s) 83, 226, 1135
AclWI GGATC 4 cut(s) 13, 568, 1291, 1411
AcoI YGGCCR 4 cut(s) 142, 151, 681, 1390
AcsI RAATTY 3 cut(s) 962, 1297, 1395
AfaI GTAC 4 cut(s) 270, 586, 781, 875
AfeI AGCGCT 1 cut(s) 459
AfiI CCNNNNNNNGG 3 cut(s) 404, 557, 1330
AflIII ACRYGT 3 cut(s) 980, 1039, 1105
AjnI CCWGG 4 cut(s) 683, 802, 998, 1348
AleI CACNNNNGTG 1 cut(s) 1237
AluBI AGCT 6 cut(s) 104, 256, 429, 788, 925, 1290
AluI AGCT 6 cut(s) 104, 256, 429, 788, 925, 1290
Alw26I GTCTC 1 cut(s) 728
AlwI GGATC 4 cut(s) 13, 568, 1291, 1411
Ama87I CYCGRG 3 cut(s) 58, 189, 201
Aor13HI TCCGGA 1 cut(s) 244
Aor51HI AGCGCT 1 cut(s) 459
AoxI GGCC 8 cut(s) 142, 151, 659, 681, 973, 996, 1078, 1390
ApoI RAATTY 3 cut(s) 962, 1297, 1395
Asp700I GAANNNNTTC 1 cut(s) 306
Asp718I GGTACC 2 cut(s) 268, 779
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
AsuC2I CCSGG 4 cut(s) 190, 191, 202, 203
AsuHPI GGTGA 6 cut(s) 190, 359, 548, 704, 1375, 1404
AsuII TTCGAA 1 cut(s) 1423
AvaI CYCGRG 3 cut(s) 58, 189, 201
AvaII GGWCC 3 cut(s) 171, 205, 1002
BalI TGGCCA 2 cut(s) 683, 1392
BanI GGYRCC 3 cut(s) 268, 779, 1097
BccI CCATC 7 cut(s) 124, 143, 242, 417, 683, 981, 1382
BceAI ACGGC 1 cut(s) 129
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 4 cut(s) 685, 804, 1000, 1350
BcnI CCSGG 4 cut(s) 190, 191, 202, 203
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 4 cut(s) 101, 461, 887, 1094
BfmI CTRYAG 1 cut(s) 1376
BfoI RGCGCY 1 cut(s) 461
BglI GCCNNNNNGGC 1 cut(s) 150
BglII AGATCT 2 cut(s) 304, 601
Bme1390I CCNGG 8 cut(s) 190, 191, 202, 203, 685, 804, 1000, 1350
Bme18I GGWCC 3 cut(s) 171, 205, 1002
BmeT110I CYCGRG 3 cut(s) 58, 189, 201
BmgT120I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
BmiI GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
BmrFI CCNGG 8 cut(s) 190, 191, 202, 203, 685, 804, 1000, 1350
BmsI GCATC 3 cut(s) 419, 1201, 1286
BpmI CTGGAG 1 cut(s) 399
Bpu14I TTCGAA 1 cut(s) 1423
BpuEI CTTGAG 1 cut(s) 1094
BpuMI CCSGG 4 cut(s) 190, 191, 202, 203
BsaJI CCNNGG 5 cut(s) 189, 201, 684, 970, 1348
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 930, 960
Bsc4I CCNNNNNNNGG 3 cut(s) 404, 557, 1330
Bse1I ACTGG 3 cut(s) 173, 239, 382
Bse3DI GCAATG 1 cut(s) 1144
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 4 cut(s) 685, 804, 1000, 1350
BseDI CCNNGG 5 cut(s) 189, 201, 684, 970, 1348
BseGI GGATG 4 cut(s) 253, 428, 694, 1166
BseLI CCNNNNNNNGG 3 cut(s) 404, 557, 1330
BseMI GCAATG 1 cut(s) 1144
BseMII CTCAG 2 cut(s) 600, 780
BseNI ACTGG 3 cut(s) 173, 239, 382
BseRI GAGGAG 4 cut(s) 40, 43, 203, 710
BseYI CCCAGC 1 cut(s) 784
BshFI GGCC 8 cut(s) 144, 153, 661, 683, 975, 998, 1080, 1392
BshNI GGYRCC 3 cut(s) 268, 779, 1097
BsiHKCI CYCGRG 3 cut(s) 58, 189, 201
BsiSI CCGG 3 cut(s) 190, 202, 245
BslI CCNNNNNNNGG 3 cut(s) 404, 557, 1330
BsmAI GTCTC 1 cut(s) 728
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 8 cut(s) 144, 153, 661, 683, 975, 998, 1080, 1392
BsoBI CYCGRG 3 cut(s) 58, 189, 201
Bsp119I TTCGAA 1 cut(s) 1423
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 7 cut(s) 5, 278, 304, 560, 601, 1283, 1403
BspACI CCGC 3 cut(s) 83, 226, 1135
BspANI GGCC 8 cut(s) 144, 153, 661, 683, 975, 998, 1080, 1392
BspCNI CTCAG 2 cut(s) 601, 781
BspEI TCCGGA 1 cut(s) 244
BspLI GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
BspPI GGATC 4 cut(s) 13, 568, 1291, 1411
BspT104I TTCGAA 1 cut(s) 1423
BspT107I GGYRCC 3 cut(s) 268, 779, 1097
BsrDI GCAATG 1 cut(s) 1144
BsrI ACTGG 3 cut(s) 173, 239, 382
BssECI CCNNGG 5 cut(s) 189, 201, 684, 970, 1348
BssMI GATC 7 cut(s) 5, 278, 304, 560, 601, 1283, 1403
BssT1I CCWWGG 1 cut(s) 970
Bst2UI CCWGG 4 cut(s) 685, 804, 1000, 1350
Bst4CI ACNGT 4 cut(s) 37, 268, 946, 1180
Bst6I CTCTTC 1 cut(s) 17
BstBI TTCGAA 1 cut(s) 1423
BstDEI CTNAG 3 cut(s) 609, 789, 1316
BstF5I GGATG 4 cut(s) 253, 428, 694, 1166
BstH2I RGCGCY 1 cut(s) 461
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 7 cut(s) 8, 281, 307, 563, 604, 1286, 1406
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 7 cut(s) 5, 278, 304, 560, 601, 1283, 1403
BstMWI GCNNNNNNNGC 2 cut(s) 101, 150
BstNI CCWGG 4 cut(s) 685, 804, 1000, 1350
BstNSI RCATGY 4 cut(s) 715, 984, 1043, 1109
BstSCI CCNGG 8 cut(s) 188, 189, 200, 201, 683, 802, 998, 1348
BstSFI CTRYAG 1 cut(s) 1376
BstX2I RGATCY 2 cut(s) 304, 601
BstYI RGATCY 2 cut(s) 304, 601
BsuRI GGCC 8 cut(s) 144, 153, 661, 683, 975, 998, 1080, 1392
BtsCI GGATG 4 cut(s) 253, 428, 694, 1166
BtsI GCAGTG 1 cut(s) 1188
BtsIMutI CAGTG 1 cut(s) 1188
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
Cfr9I CCCGGG 2 cut(s) 189, 201
Csp6I GTAC 4 cut(s) 269, 585, 780, 874
CviAII CATG 7 cut(s) 315, 712, 771, 981, 1040, 1069, 1106
CviQI GTAC 4 cut(s) 269, 585, 780, 874
DdeI CTNAG 3 cut(s) 609, 789, 1316
DpnI GATC 7 cut(s) 7, 280, 306, 562, 603, 1285, 1405
DpnII GATC 7 cut(s) 5, 278, 304, 560, 601, 1283, 1403
EaeI YGGCCR 4 cut(s) 142, 151, 681, 1390
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 970
Eco32I GATATC 1 cut(s) 1186
Eco47I GGWCC 3 cut(s) 171, 205, 1002
Eco47III AGCGCT 1 cut(s) 459
Eco88I CYCGRG 3 cut(s) 58, 189, 201
EcoO109I RGGNCCY 2 cut(s) 205, 1078
EcoRII CCWGG 4 cut(s) 683, 802, 998, 1348
EcoRV GATATC 1 cut(s) 1186
EcoT14I CCWWGG 1 cut(s) 970
ErhI CCWWGG 1 cut(s) 970
FaeI CATG 7 cut(s) 318, 715, 774, 984, 1043, 1072, 1109
FatI CATG 7 cut(s) 314, 711, 770, 980, 1039, 1068, 1105
FauI CCCGC 1 cut(s) 90
FokI GGATG 4 cut(s) 260, 435, 701, 1153
FspBI CTAG 4 cut(s) 101, 461, 887, 1094
GlaI GCGC 1 cut(s) 459
GsaI CCCAGC 1 cut(s) 788
GsuI CTGGAG 1 cut(s) 399
HaeII RGCGCY 1 cut(s) 461
HaeIII GGCC 8 cut(s) 144, 153, 661, 683, 975, 998, 1080, 1392
HapII CCGG 3 cut(s) 190, 202, 245
HhaI GCGC 1 cut(s) 460
Hin1II CATG 7 cut(s) 318, 715, 774, 984, 1043, 1072, 1109
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 4 cut(s) 162, 241, 753, 1229
HpaII CCGG 3 cut(s) 190, 202, 245
HphI GGTGA 6 cut(s) 190, 359, 548, 704, 1375, 1404
Hpy166II GTNNAC 1 cut(s) 876
Hpy188I TCNGA 7 cut(s) 76, 442, 505, 606, 642, 1210, 1443
Hpy188III TCNNGA 3 cut(s) 245, 1281, 1401
Hpy8I GTNNAC 1 cut(s) 876
HpyAV CCTTC 8 cut(s) 139, 524, 557, 620, 680, 808, 1075, 1419
HpyCH4III ACNGT 4 cut(s) 37, 268, 946, 1180
HpyCH4IV ACGT 1 cut(s) 767
HpyCH4V TGCA 9 cut(s) 390, 410, 711, 1114, 1148, 1193, 1214, 1253, 1270
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 150
HpyF3I CTNAG 3 cut(s) 609, 789, 1316
HpySE526I ACGT 1 cut(s) 767
Hsp92II CATG 7 cut(s) 318, 715, 774, 984, 1043, 1072, 1109
HspAI GCGC 1 cut(s) 458
Kpn2I TCCGGA 1 cut(s) 244
KpnI GGTACC 2 cut(s) 272, 783
Kzo9I GATC 7 cut(s) 5, 278, 304, 560, 601, 1283, 1403
LmnI GCTCC 5 cut(s) 40, 216, 380, 426, 938
LweI GCATC 3 cut(s) 419, 1201, 1286
MaeI CTAG 4 cut(s) 101, 461, 887, 1094
MaeII ACGT 1 cut(s) 767
MaeIII GTNAC 6 cut(s) 317, 716, 823, 1042, 1055, 1230
MalI GATC 7 cut(s) 7, 280, 306, 562, 603, 1285, 1405
MboI GATC 7 cut(s) 5, 278, 304, 560, 601, 1283, 1403
MboII GAAGA 6 cut(s) 34, 299, 314, 755, 851, 1036
MflI RGATCY 2 cut(s) 304, 601
MlsI TGGCCA 2 cut(s) 683, 1392
MluCI AATT 5 cut(s) 516, 962, 1157, 1297, 1395
MluNI TGGCCA 2 cut(s) 683, 1392
MlyI GAGTC 2 cut(s) 747, 1238
MmeI TCCRAC 1 cut(s) 916
Mox20I TGGCCA 2 cut(s) 683, 1392
MroI TCCGGA 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 306
MscI TGGCCA 2 cut(s) 683, 1392
MseI TTAA 1 cut(s) 1020
MslI CAYNNNNRTG 3 cut(s) 881, 985, 1237
Msp20I TGGCCA 2 cut(s) 683, 1392
MspA1I CMGCKG 1 cut(s) 788
MspI CCGG 3 cut(s) 190, 202, 245
MspR9I CCNGG 8 cut(s) 190, 191, 202, 203, 685, 804, 1000, 1350
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 4 cut(s) 685, 804, 1000, 1350
MwoI GCNNNNNNNGC 2 cut(s) 101, 150
NciI CCSGG 4 cut(s) 190, 191, 202, 203
NdeII GATC 7 cut(s) 5, 278, 304, 560, 601, 1283, 1403
NlaIII CATG 7 cut(s) 318, 715, 774, 984, 1043, 1072, 1109
NlaIV GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
NmuCI GTSAC 3 cut(s) 317, 1042, 1230
NspI RCATGY 4 cut(s) 715, 984, 1043, 1109
NspV TTCGAA 1 cut(s) 1423
OliI CACNNNNGTG 1 cut(s) 1237
PciI ACATGT 3 cut(s) 980, 1039, 1105
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 171
PctI GAATGC 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 2 cut(s) 162, 241
PflMI CCANNNNNTGG 1 cut(s) 404
PleI GAGTC 2 cut(s) 747, 1237
PpsI GAGTC 2 cut(s) 747, 1237
PpuMI RGGWCCY 1 cut(s) 205
PscI ACATGT 3 cut(s) 980, 1039, 1105
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 4 cut(s) 683, 802, 998, 1348
PspFI CCCAGC 1 cut(s) 784
PspGI CCWGG 4 cut(s) 683, 802, 998, 1348
PspN4I GGNNCC 5 cut(s) 206, 270, 781, 940, 1099
PspPI GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
PspPPI RGGWCCY 1 cut(s) 205
PsuI RGATCY 2 cut(s) 304, 601
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 4 cut(s) 270, 586, 781, 875
RsaNI GTAC 4 cut(s) 269, 585, 780, 874
RseI CAYNNNNRTG 3 cut(s) 881, 985, 1237
SaqAI TTAA 1 cut(s) 1020
Sau3AI GATC 7 cut(s) 5, 278, 304, 560, 601, 1283, 1403
Sau96I GGNCC 5 cut(s) 171, 205, 659, 1002, 1078
SchI GAGTC 2 cut(s) 747, 1238
ScrFI CCNGG 8 cut(s) 190, 191, 202, 203, 685, 804, 1000, 1350
SfaNI GCATC 3 cut(s) 419, 1201, 1286
SfcI CTRYAG 1 cut(s) 1376
SfiI GGCCNNNNNGGCC 1 cut(s) 150
SfuI TTCGAA 1 cut(s) 1423
SinI GGWCC 3 cut(s) 171, 205, 1002
SmaI CCCGGG 2 cut(s) 191, 203
SmiMI CAYNNNNRTG 3 cut(s) 881, 985, 1237
SmlI CTYRAG 1 cut(s) 1073
SmoI CTYRAG 1 cut(s) 1073
Sse9I AATT 5 cut(s) 516, 962, 1157, 1297, 1395
SsiI CCGC 3 cut(s) 83, 226, 1135
SspMI CTAG 4 cut(s) 101, 461, 887, 1094
StyD4I CCNGG 8 cut(s) 188, 189, 200, 201, 683, 802, 998, 1348
StyI CCWWGG 1 cut(s) 970
TaaI ACNGT 4 cut(s) 37, 268, 946, 1180
TaiI ACGT 1 cut(s) 770
TaqI TCGA 6 cut(s) 8, 165, 281, 702, 985, 1423
TaqII GACCGA 3 cut(s) 188, 1061, 1321
TasI AATT 5 cut(s) 516, 962, 1157, 1297, 1395
TfiI GAWTC 2 cut(s) 162, 241
Tru1I TTAA 1 cut(s) 1020
Tru9I TTAA 1 cut(s) 1020
TscAI CASTG 1 cut(s) 1195
TseFI GTSAC 3 cut(s) 317, 1042, 1230
Tsp45I GTSAC 3 cut(s) 317, 1042, 1230
TspDTI ATGAA 3 cut(s) 281, 1057, 1260
TspGWI ACGGA 1 cut(s) 1313
TspMI CCCGGG 2 cut(s) 189, 201
TspRI CASTG 1 cut(s) 1195
Van91I CCANNNNNTGG 1 cut(s) 404
VpaK11BI GGWCC 3 cut(s) 171, 205, 1002
XapI RAATTY 3 cut(s) 962, 1297, 1395
XceI RCATGY 4 cut(s) 715, 984, 1043, 1109
XmaI CCCGGG 2 cut(s) 189, 201
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 4 cut(s) 101, 461, 887, 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.