Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0017976

Physical Location & Seq

Genomic Coordinates Reverse (-)

15938834 .. 15939609

Gene Structure 776 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g12520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 357 bp

ATGGCGACGATCCAGAACGTCAAGGCTAGGCAGATCTTCGACAGCCGTGGCAATCCCACCGTCGAGGTCGATATCATTCTGTCAGATGGAACTTTGGCTAGAGCCGCTGTTCCAAGTGGTGCATCCACTGGAGTTTACGAGGCCCTTGAGTTGCGCGATGGCGCCGCTGCAATTCCTTCACTTCAAACTTGCGCTGCCAACAAACCTCGAGCTCGTCACCTTGGCCACCATGATCAAAATCAACGAAGAGCTTCATTGACCGCTGTCAAACGACTAGCCGGCCTTCAAACTCGACGCTTTACACTCTGCCTCAAGATCTGGCTGCTTCTGCACCTCCGCCATGGCTGCTGCCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004634 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005740 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0030054 GO:0031967 GO:0031975 GO:0033993 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0055044 GO:0071944 GO:0097159 GO:0097305 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

119

Amino Acids

12.86

Weight (kDa)

10.2

Isoelectric Point (pI)

42.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	4 - 55	1e-21	Enolase, N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017976)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g30481 FvH4_1g30560 FvH4_c4g00030
pyrus_communis	pycom05g12520 pycom12g01350
rosa_chinensis	RchiOBHm_Chr7g0214251
rosa_multiflora	Rmu_sc0000410.1_g000007 Rmu_sc0001154.1_g000014
rosa_roxburghii	Rroxscaffold_4G00313870 Rroxscaffold_4G00323230 Rroxscaffold_5G00382810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	161
AccII	CGCG	1 cut(s)	156
AciI	CCGC	4 cut(s)	105, 165, 261, 337
AclWI	GGATC	1 cut(s)	4
AcoI	YGGCCR	1 cut(s)	223
AcyI	GRCGYC	1 cut(s)	162
AgsI	TTSAA	2 cut(s)	185, 287
AluBI	AGCT	2 cut(s)	212, 251
AluI	AGCT	2 cut(s)	212, 251
Alw21I	GWGCWC	1 cut(s)	214
AlwI	GGATC	1 cut(s)	4
Ama87I	CYCGRG	1 cut(s)	207
AoxI	GGCC	3 cut(s)	141, 223, 280
ApeKI	GCWGC	5 cut(s)	167, 194, 322, 345, 348
Asp700I	GAANNNNTTC	1 cut(s)	250
AspLEI	GCGC	3 cut(s)	156, 164, 194
AspS9I	GGNCC	1 cut(s)	142
AsuHPI	GGTGA	1 cut(s)	209
AvaI	CYCGRG	1 cut(s)	207
BalI	TGGCCA	1 cut(s)	225
BanI	GGYRCC	1 cut(s)	161
BanII	GRGCYC	1 cut(s)	214
Bbv12I	GWGCWC	1 cut(s)	214
BbvI	GCAGC	5 cut(s)	154, 181, 309, 332, 335
BccI	CCATC	2 cut(s)	80, 152
BceAI	ACGGC	1 cut(s)	30
BclI	TGATCA	1 cut(s)	232
BfaI	CTAG	3 cut(s)	27, 99, 275
BfoI	RGCGCY	1 cut(s)	165
BglII	AGATCT	2 cut(s)	33, 315
BisI	GCNGC	7 cut(s)	105, 165, 168, 195, 323, 346, 349
BlsI	GCNGC	7 cut(s)	106, 166, 169, 196, 324, 347, 350
BmeT110I	CYCGRG	1 cut(s)	207
BmgT120I	GGNCC	1 cut(s)	142
BmiI	GGNNCC	1 cut(s)	163
BmsI	GCATC	1 cut(s)	131
BoxI	GACNNNNGTC	1 cut(s)	263
BpmI	CTGGAG	1 cut(s)	150
BpuEI	CTTGAG	2 cut(s)	167, 296
BsaBI	GATNNNNATC	1 cut(s)	237
BsaHI	GRCGYC	1 cut(s)	162
BsaJI	CCNNGG	3 cut(s)	46, 220, 340
Bse118I	RCCGGY	1 cut(s)	278
Bse1I	ACTGG	1 cut(s)	133
Bse8I	GATNNNNATC	1 cut(s)	237
BseDI	CCNNGG	3 cut(s)	46, 220, 340
BseGI	GGATG	1 cut(s)	122
BseJI	GATNNNNATC	1 cut(s)	237
BseNI	ACTGG	1 cut(s)	133
BseXI	GCAGC	5 cut(s)	154, 181, 309, 332, 335
BsgI	GTGCAG	1 cut(s)	314
Bsh1236I	CGCG	1 cut(s)	156
BshFI	GGCC	3 cut(s)	143, 225, 282
BshNI	GGYRCC	1 cut(s)	161
BsiHKAI	GWGCWC	1 cut(s)	214
BsiHKCI	CYCGRG	1 cut(s)	207
BsiSI	CCGG	1 cut(s)	279
BsnI	GGCC	3 cut(s)	143, 225, 282
BsoBI	CYCGRG	1 cut(s)	207
Bsp1286I	GDGCHC	1 cut(s)	214
Bsp143I	GATC	4 cut(s)	9, 33, 232, 315
Bsp19I	CCATGG	1 cut(s)	340
BspACI	CCGC	4 cut(s)	105, 165, 261, 337
BspANI	GGCC	3 cut(s)	143, 225, 282
BspFNI	CGCG	1 cut(s)	156
BspLI	GGNNCC	1 cut(s)	163
BspPI	GGATC	1 cut(s)	4
BspQI	GCTCTTC	1 cut(s)	241
BspT107I	GGYRCC	1 cut(s)	161
BsrFI	RCCGGY	1 cut(s)	278
BsrI	ACTGG	1 cut(s)	133
BssAI	RCCGGY	1 cut(s)	278
BssECI	CCNNGG	3 cut(s)	46, 220, 340
BssMI	GATC	4 cut(s)	9, 33, 232, 315
BssNI	GRCGYC	1 cut(s)	162
BssT1I	CCWWGG	2 cut(s)	220, 340
Bst4CI	ACNGT	1 cut(s)	61
Bst6I	CTCTTC	1 cut(s)	241
BstACI	GRCGYC	1 cut(s)	162
BstC8I	GCNNGC	1 cut(s)	280
BstDSI	CCRYGG	2 cut(s)	46, 340
BstF5I	GGATG	1 cut(s)	122
BstFNI	CGCG	1 cut(s)	156
BstH2I	RGCGCY	1 cut(s)	165
BstHHI	GCGC	3 cut(s)	156, 164, 194
BstKTI	GATC	4 cut(s)	12, 36, 235, 318
BstMBI	GATC	4 cut(s)	9, 33, 232, 315
BstMWI	GCNNNNNNNGC	3 cut(s)	104, 328, 345
BstPAI	GACNNNNGTC	1 cut(s)	263
BstUI	CGCG	1 cut(s)	156
BstV1I	GCAGC	5 cut(s)	154, 181, 309, 332, 335
BstX2I	RGATCY	2 cut(s)	33, 315
BstYI	RGATCY	2 cut(s)	33, 315
BsuRI	GGCC	3 cut(s)	143, 225, 282
BtgI	CCRYGG	2 cut(s)	46, 340
BtgZI	GCGATG	1 cut(s)	171
BtsCI	GGATG	1 cut(s)	122
BtsIMutI	CAGTG	1 cut(s)	126
Cac8I	GCNNGC	1 cut(s)	280
CfoI	GCGC	3 cut(s)	156, 164, 194
Cfr10I	RCCGGY	1 cut(s)	278
Cfr13I	GGNCC	1 cut(s)	142
CseI	GACGC	1 cut(s)	303
CviAII	CATG	2 cut(s)	230, 341
DinI	GGCGCC	1 cut(s)	163
DpnI	GATC	4 cut(s)	11, 35, 234, 317
DpnII	GATC	4 cut(s)	9, 33, 232, 315
EaeI	YGGCCR	1 cut(s)	223
Eam1104I	CTCTTC	1 cut(s)	241
EarI	CTCTTC	1 cut(s)	241
EciI	GGCGGA	1 cut(s)	326
Ecl136II	GAGCTC	1 cut(s)	212
Eco130I	CCWWGG	2 cut(s)	220, 340
Eco24I	GRGCYC	1 cut(s)	214
Eco32I	GATATC	1 cut(s)	73
Eco53kI	GAGCTC	1 cut(s)	212
Eco88I	CYCGRG	1 cut(s)	207
EcoICRI	GAGCTC	1 cut(s)	212
EcoO109I	RGGNCCY	1 cut(s)	142
EcoRV	GATATC	1 cut(s)	73
EcoT14I	CCWWGG	2 cut(s)	220, 340
EcoT38I	GRGCYC	1 cut(s)	214
EgeI	GGCGCC	1 cut(s)	163
EheI	GGCGCC	1 cut(s)	163
ErhI	CCWWGG	2 cut(s)	220, 340
FaeI	CATG	2 cut(s)	233, 344
FaiI	YATR	2 cut(s)	231, 342
FatI	CATG	2 cut(s)	229, 340
FbaI	TGATCA	1 cut(s)	232
Fnu4HI	GCNGC	7 cut(s)	105, 165, 168, 195, 323, 346, 349
FokI	GGATG	1 cut(s)	109
FriOI	GRGCYC	1 cut(s)	214
Fsp4HI	GCNGC	7 cut(s)	105, 165, 168, 195, 323, 346, 349
FspBI	CTAG	3 cut(s)	27, 99, 275
GlaI	GCGC	3 cut(s)	155, 163, 193
GluI	GCNGC	7 cut(s)	105, 165, 168, 195, 323, 346, 349
GsuI	CTGGAG	1 cut(s)	150
HaeII	RGCGCY	1 cut(s)	165
HaeIII	GGCC	3 cut(s)	143, 225, 282
HapII	CCGG	1 cut(s)	279
HgaI	GACGC	1 cut(s)	303
HhaI	GCGC	3 cut(s)	156, 164, 194
Hin1I	GRCGYC	1 cut(s)	162
Hin1II	CATG	2 cut(s)	233, 344
Hin6I	GCGC	3 cut(s)	154, 162, 192
HinP1I	GCGC	3 cut(s)	154, 162, 192
HpaII	CCGG	1 cut(s)	279
HphI	GGTGA	1 cut(s)	209
Hpy166II	GTNNAC	1 cut(s)	136
Hpy188I	TCNGA	1 cut(s)	85
Hpy188III	TCNNGA	2 cut(s)	13, 313
Hpy8I	GTNNAC	1 cut(s)	136
Hpy99I	CGWCG	3 cut(s)	10, 65, 297
HpyAV	CCTTC	2 cut(s)	186, 293
HpyCH4III	ACNGT	1 cut(s)	61
HpyCH4IV	ACGT	1 cut(s)	18
HpyCH4V	TGCA	3 cut(s)	122, 170, 331
HpyF10VI	GCNNNNNNNGC	3 cut(s)	104, 328, 345
HpySE526I	ACGT	1 cut(s)	18
Hsp92I	GRCGYC	1 cut(s)	162
Hsp92II	CATG	2 cut(s)	233, 344
HspAI	GCGC	3 cut(s)	154, 162, 192
KasI	GGCGCC	1 cut(s)	161
KroI	GCCGGC	1 cut(s)	278
KroNI	GCCGGC	1 cut(s)	280
Ksp22I	TGATCA	1 cut(s)	232
Kzo9I	GATC	4 cut(s)	9, 33, 232, 315
LguI	GCTCTTC	1 cut(s)	241
LpnPI	CCDG	4 cut(s)	26, 114, 292, 304
Lsp1109I	GCAGC	5 cut(s)	154, 181, 309, 332, 335
LweI	GCATC	1 cut(s)	131
MaeI	CTAG	3 cut(s)	27, 99, 275
MaeII	ACGT	1 cut(s)	18
MaeIII	GTNAC	1 cut(s)	215
MalI	GATC	4 cut(s)	11, 35, 234, 317
MboI	GATC	4 cut(s)	9, 33, 232, 315
MboII	GAAGA	2 cut(s)	28, 258
MflI	RGATCY	2 cut(s)	33, 315
MhlI	GDGCHC	1 cut(s)	214
MlsI	TGGCCA	1 cut(s)	225
MluCI	AATT	1 cut(s)	171
MluNI	TGGCCA	1 cut(s)	225
Mly113I	GGCGCC	1 cut(s)	162
MnlI	CCTC	5 cut(s)	58, 133, 216, 320, 344
Mox20I	TGGCCA	1 cut(s)	225
MroNI	GCCGGC	1 cut(s)	278
MroXI	GAANNNNTTC	1 cut(s)	250
MscI	TGGCCA	1 cut(s)	225
Msp20I	TGGCCA	1 cut(s)	225
MspA1I	CMGCKG	3 cut(s)	107, 167, 263
MspI	CCGG	1 cut(s)	279
MvnI	CGCG	1 cut(s)	156
MwoI	GCNNNNNNNGC	3 cut(s)	104, 328, 345
NaeI	GCCGGC	1 cut(s)	280
NarI	GGCGCC	1 cut(s)	162
NcoI	CCATGG	1 cut(s)	340
NdeII	GATC	4 cut(s)	9, 33, 232, 315
NgoMIV	GCCGGC	1 cut(s)	278
NlaIII	CATG	2 cut(s)	233, 344
NlaIV	GGNNCC	1 cut(s)	163
NmuCI	GTSAC	1 cut(s)	215
PaeR7I	CTCGAG	1 cut(s)	207
PciSI	GCTCTTC	1 cut(s)	241
PdiI	GCCGGC	1 cut(s)	280
PdmI	GAANNNNTTC	1 cut(s)	250
PkrI	GCNGC	7 cut(s)	106, 166, 169, 196, 324, 347, 350
PluTI	GGCGCC	1 cut(s)	165
PshAI	GACNNNNGTC	1 cut(s)	263
Psp124BI	GAGCTC	1 cut(s)	214
PspN4I	GGNNCC	1 cut(s)	163
PspPI	GGNCC	1 cut(s)	142
PspXI	VCTCGAGB	1 cut(s)	207
PsuI	RGATCY	2 cut(s)	33, 315
SacI	GAGCTC	1 cut(s)	214
SapI	GCTCTTC	1 cut(s)	241
SatI	GCNGC	7 cut(s)	105, 165, 168, 195, 323, 346, 349
Sau3AI	GATC	4 cut(s)	9, 33, 232, 315
Sau96I	GGNCC	1 cut(s)	142
SduI	GDGCHC	1 cut(s)	214
SetI	ASST	7 cut(s)	21, 69, 208, 214, 222, 253, 336
SfaNI	GCATC	1 cut(s)	131
SfoI	GGCGCC	1 cut(s)	163
Sfr274I	CTCGAG	1 cut(s)	207
SlaI	CTCGAG	1 cut(s)	207
SmlI	CTYRAG	3 cut(s)	146, 207, 311
SmoI	CTYRAG	3 cut(s)	146, 207, 311
Sse9I	AATT	1 cut(s)	171
SsiI	CCGC	4 cut(s)	105, 165, 261, 337
SspDI	GGCGCC	1 cut(s)	161
SspMI	CTAG	3 cut(s)	27, 99, 275
SstI	GAGCTC	1 cut(s)	214
StyI	CCWWGG	2 cut(s)	220, 340
TaaI	ACNGT	1 cut(s)	61
TaiI	ACGT	1 cut(s)	21
TaqI	TCGA	5 cut(s)	39, 63, 69, 208, 292
TasI	AATT	1 cut(s)	171
TauI	GCSGC	2 cut(s)	107, 167
TscAI	CASTG	1 cut(s)	133
TseFI	GTSAC	1 cut(s)	215
TseI	GCWGC	5 cut(s)	167, 194, 322, 345, 348
Tsp45I	GTSAC	1 cut(s)	215
TspDTI	ATGAA	1 cut(s)	243
TspRI	CASTG	1 cut(s)	133
XhoI	CTCGAG	1 cut(s)	207
XmnI	GAANNNNTTC	1 cut(s)	250
XspI	CTAG	3 cut(s)	27, 99, 275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.