Rroxscaffold_5G00382810

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017976
Physical Location & Seq
Reverse (-)
62592314 .. 62593807
1494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00382810.1

Sequence Viewer

Length: 1236 bp
ATGAAAGTCATGCTTCGATGGACCTTCATATCGAGTGGAATTGAAAATATGGTATTGGTTGATGGTAACCTCTTCTCTCGGGTAACGATTCTAATTTCTGGATTGAAAGCTAATTTGTTGAGTCAAACCCGGGTTGAGTCACCCGTGCCATTCCCATTGTCAATACTTGAATCTTTCAAGCATGTTGTAAAAGCTTGGTTGAGGTATTCTTCTATTCATCCAGTTGTTTTATCGACTACTATTGCTACAGCATTGTTACCAAGTTTAAAAGTTTGGGTGCTTAACTTGCATGGTTTAACCGGGAGCAAGGCTGAATTGTTATTTGGTGGTGCATTCCCGATAAGAGAGCTCATGTTTGTTAACGAATGTCACATTGTTCCCACCGGAACAATTGATAAAGCAATTGATAAAAGACTTGTTTGTCATTTGGGTGAAGAGTTGCACGATCTTATGATTATGGAAGATGAGATCATGCATTCTGCTTTCACCGTGATTTGCCAAAGCACAATAGAGCAGCCTAAAATTGATGAGTACATAGTACAAAATTTTGAGGGGGCTCTTTCCTCGCGGGGTTGTAGTTCAACCGCAAATGGTAAAGCTATGATGGAGTTGTATGTGGGGTTGTATATAAATATTGGATTCCATGATTTTGCACTAAATTCTCGCAGTCCAACACTTGGATTTGAGTTGTTTGCAAATATGAAGTTCATAAACGTACCGTGCAAGAACATGCTTTGCAAGATTTTGAACCAAATGGATCCTAAGGACATCATTGCTTCGGGAGCTACCAATCAAAGCCACATGCTACATGACAAGGCTACCGAATTTTCCCGTGAACTCAATCAAGCTAATGCATGTACACTATTGAGTAATTATGGTCATGGGAAGATGAAAAGATTAGTGCAAGAGAGTGAAGAGACCAATGGAAAATGTACCGCACCTGCAATTACTCCAATAGTTTCGAAGGAAATGAACAGTCAAGACTCATGCCGGAAGTTCAACTTTGAATGTGGACAGAGTAAAAGTATCTCATCACTTGGAGGACAAATCGATGTGCTCGCAGCCACACAAAGGACCAACCTTGAAGAGAAAAATGACCTGTTCCCTTACTTTCTTCATTGGGGTATCACATTGATCGTCTTCTACTCGAGTTTGTCTCTCGCTATCTCCAATTATGATTGCTTCTCGTATAACAATTTGGGTGCTGCTTTTGACAATACGGTGACTCTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

411

Amino Acids

45.71

Weight (kDa)

6.23

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 949
AasI GACNNNNNNGTC 1 cut(s) 420
Acc36I ACCTGC 1 cut(s) 949
AccB7I CCANNNNNTGG 1 cut(s) 677
AccII CGCG 1 cut(s) 568
AciI CCGC 3 cut(s) 568, 585, 936
AclWI GGATC 2 cut(s) 752, 765
AcsI RAATTY 3 cut(s) 544, 658, 824
AfaI GTAC 5 cut(s) 533, 540, 717, 859, 934
AfiI CCNNNNNNNGG 2 cut(s) 677, 1071
AgsI TTSAA 9 cut(s) 44, 106, 170, 178, 582, 748, 1000, 1007, 1085
AjuI GAANNNNNNNTTGG 2 cut(s) 306, 338
AluBI AGCT 6 cut(s) 110, 194, 349, 599, 785, 848
AluI AGCT 6 cut(s) 110, 194, 349, 599, 785, 848
Alw21I GWGCWC 2 cut(s) 351, 1059
Alw26I GTCTC 2 cut(s) 911, 1161
AlwI GGATC 2 cut(s) 752, 765
Ama87I CYCGRG 3 cut(s) 78, 129, 1147
ApeKI GCWGC 3 cut(s) 514, 1061, 1205
ApoI RAATTY 3 cut(s) 544, 658, 824
AspS9I GGNCC 2 cut(s) 21, 1074
AsuC2I CCSGG 3 cut(s) 130, 131, 301
AsuHPI GGTGA 4 cut(s) 132, 443, 478, 1234
AsuII TTCGAA 1 cut(s) 962
AvaI CYCGRG 3 cut(s) 78, 129, 1147
AvaII GGWCC 2 cut(s) 21, 1074
AxyI CCTNAGG 1 cut(s) 762
BamHI GGATCC 1 cut(s) 757
BanII GRGCYC 2 cut(s) 351, 559
BbsI GAAGAC 1 cut(s) 1132
Bbv12I GWGCWC 2 cut(s) 351, 1059
BbvI GCAGC 3 cut(s) 526, 1073, 1192
BccI CCATC 3 cut(s) 12, 56, 598
BcnI CCSGG 3 cut(s) 130, 131, 301
BcoDI GTCTC 2 cut(s) 911, 1161
BfaI CTAG 1 cut(s) 1234
BfmI CTRYAG 1 cut(s) 246
BfuAI ACCTGC 1 cut(s) 949
BisI GCNGC 3 cut(s) 515, 1062, 1206
BlsI GCNGC 3 cut(s) 516, 1063, 1207
Bme1390I CCNGG 3 cut(s) 130, 131, 301
Bme18I GGWCC 2 cut(s) 21, 1074
BmeT110I CYCGRG 3 cut(s) 78, 129, 1147
BmgT120I GGNCC 2 cut(s) 21, 1074
BmiI GGNNCC 1 cut(s) 759
BmrFI CCNGG 3 cut(s) 130, 131, 301
BpiI GAAGAC 1 cut(s) 1132
BplI GAGNNNNNCTC 2 cut(s) 1141, 1173
Bpu14I TTCGAA 1 cut(s) 962
BpuMI CCSGG 3 cut(s) 130, 131, 301
Bsa29I ATCGAT 1 cut(s) 1050
BsaI GGTCTC 1 cut(s) 911
BsaJI CCNNGG 1 cut(s) 129
BsaWI WCCGGW 1 cut(s) 383
Bsc4I CCNNNNNNNGG 2 cut(s) 677, 1071
Bse1I ACTGG 1 cut(s) 221
Bse21I CCTNAGG 1 cut(s) 762
Bse3DI GCAATG 1 cut(s) 771
BseCI ATCGAT 1 cut(s) 1050
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 2 cut(s) 677, 1071
BseMI GCAATG 1 cut(s) 771
BseNI ACTGG 1 cut(s) 221
BseXI GCAGC 3 cut(s) 526, 1073, 1192
Bsh1236I CGCG 1 cut(s) 568
BshVI ATCGAT 1 cut(s) 1050
BsiHKAI GWGCWC 2 cut(s) 351, 1059
BsiHKCI CYCGRG 3 cut(s) 78, 129, 1147
BsiSI CCGG 4 cut(s) 130, 300, 384, 991
BslI CCNNNNNNNGG 2 cut(s) 677, 1071
BsmAI GTCTC 2 cut(s) 911, 1161
BsmI GAATGC 2 cut(s) 332, 475
Bso31I GGTCTC 1 cut(s) 911
BsoBI CYCGRG 3 cut(s) 78, 129, 1147
Bsp119I TTCGAA 1 cut(s) 962
Bsp1286I GDGCHC 3 cut(s) 351, 559, 1059
Bsp1407I TGTACA 1 cut(s) 857
Bsp143I GATC 4 cut(s) 445, 468, 757, 1134
BspACI CCGC 3 cut(s) 568, 585, 936
BspDI ATCGAT 1 cut(s) 1050
BspFNI CGCG 1 cut(s) 568
BspLI GGNNCC 1 cut(s) 759
BspMI ACCTGC 1 cut(s) 949
BspPI GGATC 2 cut(s) 752, 765
BspT104I TTCGAA 1 cut(s) 962
BspTNI GGTCTC 1 cut(s) 911
BsrDI GCAATG 1 cut(s) 771
BsrGI TGTACA 1 cut(s) 857
BsrI ACTGG 1 cut(s) 221
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 4 cut(s) 445, 468, 757, 1134
Bst4CI ACNGT 4 cut(s) 490, 720, 977, 1222
Bst6I CTCTTC 4 cut(s) 77, 429, 909, 1080
BstAUI TGTACA 1 cut(s) 857
BstBI TTCGAA 1 cut(s) 962
BstC8I GCNNGC 1 cut(s) 1059
BstDEI CTNAG 1 cut(s) 762
BstEII GGTNACC 1 cut(s) 65
BstF5I GGATG 1 cut(s) 217
BstFNI CGCG 1 cut(s) 568
BstKTI GATC 4 cut(s) 448, 471, 760, 1137
BstMAI GTCTC 2 cut(s) 911, 1161
BstMBI GATC 4 cut(s) 445, 468, 757, 1134
BstMWI GCNNNNNNNGC 2 cut(s) 286, 782
BstNSI RCATGY 4 cut(s) 185, 733, 805, 858
BstPI GGTNACC 1 cut(s) 65
BstSCI CCNGG 3 cut(s) 128, 129, 299
BstSFI CTRYAG 1 cut(s) 246
BstUI CGCG 1 cut(s) 568
BstV1I GCAGC 3 cut(s) 526, 1073, 1192
BstV2I GAAGAC 1 cut(s) 1132
BstX2I RGATCY 1 cut(s) 757
BstYI RGATCY 1 cut(s) 757
Bsu15I ATCGAT 1 cut(s) 1050
Bsu36I CCTNAGG 1 cut(s) 762
BsuTUI ATCGAT 1 cut(s) 1050
BtsCI GGATG 1 cut(s) 217
BveI ACCTGC 1 cut(s) 949
Cac8I GCNNGC 1 cut(s) 1059
Cfr13I GGNCC 2 cut(s) 21, 1074
Cfr9I CCCGGG 1 cut(s) 129
ClaI ATCGAT 1 cut(s) 1050
Csp6I GTAC 5 cut(s) 532, 539, 716, 858, 933
CviQI GTAC 5 cut(s) 532, 539, 716, 858, 933
DdeI CTNAG 1 cut(s) 762
DpnI GATC 4 cut(s) 447, 470, 759, 1136
DpnII GATC 4 cut(s) 445, 468, 757, 1134
DraI TTTAAA 1 cut(s) 267
DrdI GACNNNNNNGTC 1 cut(s) 420
DseDI GACNNNNNNGTC 1 cut(s) 420
Eam1104I CTCTTC 4 cut(s) 77, 429, 909, 1080
EarI CTCTTC 4 cut(s) 77, 429, 909, 1080
Ecl136II GAGCTC 1 cut(s) 349
Eco24I GRGCYC 2 cut(s) 351, 559
Eco31I GGTCTC 1 cut(s) 911
Eco47I GGWCC 2 cut(s) 21, 1074
Eco53kI GAGCTC 1 cut(s) 349
Eco81I CCTNAGG 1 cut(s) 762
Eco88I CYCGRG 3 cut(s) 78, 129, 1147
Eco91I GGTNACC 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 349
EcoO65I GGTNACC 1 cut(s) 65
EcoT22I ATGCAT 2 cut(s) 477, 856
EcoT38I GRGCYC 2 cut(s) 351, 559
FalI AAGNNNNNCTT 3 cut(s) 29, 986, 1018
FauI CCCGC 1 cut(s) 561
Fnu4HI GCNGC 3 cut(s) 515, 1062, 1206
FokI GGATG 1 cut(s) 204
FriOI GRGCYC 2 cut(s) 351, 559
Fsp4HI GCNGC 3 cut(s) 515, 1062, 1206
FspBI CTAG 1 cut(s) 1234
GluI GCNGC 3 cut(s) 515, 1062, 1206
HapII CCGG 4 cut(s) 130, 300, 384, 991
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 7 cut(s) 88, 121, 137, 170, 639, 983, 1225
HpaI GTTAAC 1 cut(s) 361
HpaII CCGG 4 cut(s) 130, 300, 384, 991
HphI GGTGA 4 cut(s) 132, 443, 478, 1234
Hpy166II GTNNAC 4 cut(s) 361, 836, 860, 1013
Hpy188III TCNNGA 4 cut(s) 99, 337, 780, 980
Hpy8I GTNNAC 4 cut(s) 361, 836, 860, 1013
HpyAV CCTTC 2 cut(s) 34, 958
HpyCH4III ACNGT 4 cut(s) 490, 720, 977, 1222
HpyCH4IV ACGT 1 cut(s) 714
HpyF10VI GCNNNNNNNGC 2 cut(s) 286, 782
HpyF3I CTNAG 1 cut(s) 762
HpySE526I ACGT 1 cut(s) 714
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 4 cut(s) 445, 468, 757, 1134
LmnI GCTCC 2 cut(s) 303, 782
LpnPI CCDG 8 cut(s) 84, 143, 234, 313, 397, 954, 1004, 1112
Lsp1109I GCAGC 3 cut(s) 526, 1073, 1192
MaeI CTAG 1 cut(s) 1234
MaeII ACGT 1 cut(s) 714
MaeIII GTNAC 6 cut(s) 65, 82, 138, 255, 368, 1222
MalI GATC 4 cut(s) 447, 470, 759, 1136
MboI GATC 4 cut(s) 445, 468, 757, 1134
MboII GAAGA 9 cut(s) 64, 201, 446, 473, 898, 926, 1097, 1106, 1132
MfeI CAATTG 2 cut(s) 390, 402
MflI RGATCY 1 cut(s) 757
MhlI GDGCHC 3 cut(s) 351, 559, 1059
MlyI GAGTC 4 cut(s) 130, 146, 977, 1219
MmeI TCCRAC 1 cut(s) 695
MnlI CCTC 5 cut(s) 80, 195, 544, 574, 1034
Mph1103I ATGCAT 2 cut(s) 477, 856
MseI TTAA 4 cut(s) 266, 282, 296, 360
MslI CAYNNNNRTG 1 cut(s) 429
MspI CCGG 4 cut(s) 130, 300, 384, 991
MspR9I CCNGG 3 cut(s) 130, 131, 301
MunI CAATTG 2 cut(s) 390, 402
Mva1269I GAATGC 2 cut(s) 332, 475
MvnI CGCG 1 cut(s) 568
MwoI GCNNNNNNNGC 2 cut(s) 286, 782
NciI CCSGG 3 cut(s) 130, 131, 301
NdeII GATC 4 cut(s) 445, 468, 757, 1134
NlaIV GGNNCC 1 cut(s) 759
NmuCI GTSAC 3 cut(s) 138, 368, 1222
NsiI ATGCAT 2 cut(s) 477, 856
NspI RCATGY 4 cut(s) 185, 733, 805, 858
NspV TTCGAA 1 cut(s) 962
PaeR7I CTCGAG 1 cut(s) 1147
PaqCI CACCTGC 1 cut(s) 949
PctI GAATGC 2 cut(s) 332, 475
PfeI GAWTC 3 cut(s) 88, 170, 639
PflMI CCANNNNNTGG 1 cut(s) 677
PkrI GCNGC 3 cut(s) 516, 1063, 1207
PleI GAGTC 4 cut(s) 129, 145, 977, 1219
PpsI GAGTC 4 cut(s) 129, 145, 977, 1219
Psp124BI GAGCTC 1 cut(s) 351
PspEI GGTNACC 1 cut(s) 65
PspN4I GGNNCC 1 cut(s) 759
PspPI GGNCC 2 cut(s) 21, 1074
PspXI VCTCGAGB 1 cut(s) 1147
PsuI RGATCY 1 cut(s) 757
RsaI GTAC 5 cut(s) 533, 540, 717, 859, 934
RsaNI GTAC 5 cut(s) 532, 539, 716, 858, 933
RseI CAYNNNNRTG 1 cut(s) 429
SacI GAGCTC 1 cut(s) 351
SaqAI TTAA 4 cut(s) 266, 282, 296, 360
SatI GCNGC 3 cut(s) 515, 1062, 1206
Sau3AI GATC 4 cut(s) 445, 468, 757, 1134
Sau96I GGNCC 2 cut(s) 21, 1074
SchI GAGTC 4 cut(s) 130, 146, 977, 1219
ScrFI CCNGG 3 cut(s) 130, 131, 301
SduI GDGCHC 3 cut(s) 351, 559, 1059
SfcI CTRYAG 1 cut(s) 246
Sfr274I CTCGAG 1 cut(s) 1147
SfuI TTCGAA 1 cut(s) 962
SinI GGWCC 2 cut(s) 21, 1074
SlaI CTCGAG 1 cut(s) 1147
SmaI CCCGGG 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 429
SmlI CTYRAG 1 cut(s) 1147
SmoI CTYRAG 1 cut(s) 1147
SsiI CCGC 3 cut(s) 568, 585, 936
SspI AATATT 1 cut(s) 634
SspMI CTAG 1 cut(s) 1234
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 3 cut(s) 128, 129, 299
TaaI ACNGT 4 cut(s) 490, 720, 977, 1222
TaiI ACGT 1 cut(s) 717
TaqI TCGA 6 cut(s) 16, 32, 233, 962, 1050, 1148
TatI WGTACW 3 cut(s) 531, 538, 857
TfiI GAWTC 3 cut(s) 88, 170, 639
Tru1I TTAA 4 cut(s) 266, 282, 296, 360
Tru9I TTAA 4 cut(s) 266, 282, 296, 360
TseFI GTSAC 3 cut(s) 138, 368, 1222
TseI GCWGC 3 cut(s) 514, 1061, 1205
Tsp45I GTSAC 3 cut(s) 138, 368, 1222
TspDTI ATGAA 8 cut(s) 16, 17, 206, 697, 716, 905, 986, 1106
TspMI CCCGGG 1 cut(s) 129
Van91I CCANNNNNTGG 1 cut(s) 677
VpaK11BI GGWCC 2 cut(s) 21, 1074
XapI RAATTY 3 cut(s) 544, 658, 824
XceI RCATGY 4 cut(s) 185, 733, 805, 858
XhoI CTCGAG 1 cut(s) 1147
XmaI CCCGGG 1 cut(s) 129
XspI CTAG 1 cut(s) 1234
Zsp2I ATGCAT 2 cut(s) 477, 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.