pycom05g12960

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010779
Physical Location & Seq
Reverse (-)
16500458 .. 16503672
3215 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1782 bp
ATGTCACCGGCGATGAAGAAGAAGAAGAGAGACGCAAACAACGGTCACCGCTGCGTCTATATTAACGACGTTATCACCGCTGCCGCCATAGGCGGCGTCGTTTTCAGAACCTCTATTCTTCGGCGATACCTCCGCCGTCTCCGCCACCACGTGTTCCCGCTAATCTGCGCCGTATCCGGTTGCCTGCTTATCTTCCTCGCTGCCTTCTCTCTCCTCGCTCCGTCTCCGGTTCTGCATTTTCACAATCACCTCTCGCTCTTGCGTCACCTCTCCTCGGTACTCAAGCACACACCGAATCACCTCTCAGAGAGCGGAGGAAGTTCAGGTCGAGATTTATGGAGTACAAGGAATTCGGAGGTTTTCTTCGGTTGCAGCAATGCCGCCGATAACTTTGCAACTGCTGATGCGAAAACTCATTCGAATCGTTACTTGTTGATTGCTACAAGTGGAGGTTTGAACCAACAAAGAACAGGGATAACAGATGCTGTCGTTGCAGCTTATATCTTGAATGCTACTCTTGTTGTTCCCAAGCTGGACCAGAAATCGTATTGGAAGGATTCCAGTAACTTTGATGAAATCTTTGACGTAGACTGGTTTATATCATCTCTATCAAAAGATGTTAAAATCATAAAACAGCTTCCAACAAAGGGAGGGAAACCAATGAGCCCATATACCATGCGAGTTCCGAGGAAGTGTAATGCAAAATGTTATCAGAATCGCTTGGTGCCCGTTCTTAATAAAAAGCATGCTGTTCAGCTCACAAAATTTGATTACAGACTTTCAAATAAGTTGGATTCAAATTTACAAAAACTGAGATGTAGAGCCAATTATCAAGCCTTGAAATTTACCGACACGATTAGAAAAATGGGTAAAAAGTTGGTTGAGAGAATGAGCAAGAAAAACAAGCATTTCATTGCCCTGCATTTGAGGTTTGAGCCGGATATGCTGGCGTTTTCTGGATGCGATTATGGTGGGGGAGAAAAAGAAAGGAAAGAGCTTGGCAAACTTCGGAAAAGGTGGAAAACTTTACATGCAAGCAACCCTGACAAGGTACGAAGACATGGCAGATGCCCGCTAACTCCAGAGGAAGTTGGTCTCATGCTTAGAGCGTTGGGTTTCGGACGTGATGTTCACTTGTATGTGGCATCCGGTGATGTATATGGAGGTGAAGAGACATTGGCACCCCTAAAATCACTTTTCCCAAACTTCCACACAAAAGAGACTCTGTTAAGCAGGGAGGAGTTGGCCCCGTTTTCATCCTTCTCTTCTCGAATGGCTGCGCTGGACTTTATTGTTTGCGATGAAAGTGATGTTTTCGTCACAAACAACAATGGCAACATGGCTAAAATGTTAGCTGGTCGAAGGAGATACTTTGGTCACAAACCAACAATCCGTCCCAATGCTAAGAAACTGTCTCAGTTGTTCATAAATCGTAATAACATGACATGGGAAGAATTTGCATCGAGGATTCGAACAGATCAAATTGGATTCTTGGGAGAGCCAAATGAGGTAAAGCCGGGAAGGGGTGTGTTCCACGAAAACCCCGCTGCCTGCATTTGTAGCACATCTGACACAAAGGCTAAGGAGGATTATATGAACAAGGACAGTGGTTATGCGACAGATGAGCAAGTAACCGAGGACGAGCAAGATTGGTCTGAGATGGATTATGCTGAAATTACAAATCAGTCTATAGGGAAAGTGCTGCCTAGTGGAAGCGTTTCAGGTCCAAGTGTTCTACTTAAATCCGACCAACCAGAATTCGAAGAGTTCTTTTCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

594

Amino Acids

66.85

Weight (kDa)

9.43

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 143 - 457 1.2e-102 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 724, 1180
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 588
AcsI RAATTY 6 cut(s) 349, 764, 799, 842, 1454, 1757
AcvI CACGTG 1 cut(s) 151
AcyI GRCGYC 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 151
AfaI GTAC 3 cut(s) 279, 343, 1053
AfiI CCNNNNNNNGG 4 cut(s) 274, 647, 1048, 1523
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 5 cut(s) 457, 508, 783, 798, 841
AjiI CACGTC 1 cut(s) 1124
AloI GAACNNNNNNTCC 2 cut(s) 1113, 1145
AluBI AGCT 6 cut(s) 497, 532, 637, 757, 997, 1355
AluI AGCT 6 cut(s) 497, 532, 637, 757, 997, 1355
Alw26I GTCTC 7 cut(s) 24, 143, 228, 1100, 1166, 1214, 1419
AlwNI CAGNNNCTG 1 cut(s) 485
AoxI GGCC 1 cut(s) 1245
ApeKI GCWGC 8 cut(s) 51, 80, 200, 372, 494, 1277, 1547, 1702
ApoI RAATTY 6 cut(s) 349, 764, 799, 842, 1454, 1757
Asp700I GAANNNNTTC 1 cut(s) 1717
AspLEI GCGC 2 cut(s) 170, 1282
AspS9I GGNCC 3 cut(s) 535, 1246, 1724
AsuC2I CCSGG 1 cut(s) 1518
AsuHPI GGTGA 7 cut(s) 38, 67, 239, 257, 290, 1163, 1178
AsuII TTCGAA 3 cut(s) 419, 1471, 1761
AvaII GGWCC 2 cut(s) 535, 1724
BaeGI GKGCMC 1 cut(s) 729
BaeI ACNNNNGTAYC 2 cut(s) 1360, 1393
BanI GGYRCC 2 cut(s) 724, 1180
BanII GRGCYC 1 cut(s) 668
BbrPI CACGTG 1 cut(s) 151
BbsI GAAGAC 1 cut(s) 1063
BbvI GCAGC 8 cut(s) 38, 67, 187, 384, 506, 1264, 1534, 1689
BccI CCATC 1 cut(s) 1654
BceAI ACGGC 2 cut(s) 120, 155
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciVI GTATCC 1 cut(s) 184
BcnI CCSGG 1 cut(s) 1518
BcoDI GTCTC 7 cut(s) 24, 143, 228, 1100, 1166, 1214, 1419
BfaI CTAG 1 cut(s) 1707
BfmI CTRYAG 1 cut(s) 1689
BfuI GTATCC 1 cut(s) 184
Bme1390I CCNGG 1 cut(s) 1518
Bme18I GGWCC 2 cut(s) 535, 1724
BmgBI CACGTC 1 cut(s) 1124
BmgT120I GGNCC 3 cut(s) 535, 1246, 1724
BmiI GGNNCC 3 cut(s) 726, 1182, 1248
BmrFI CCNGG 1 cut(s) 1518
BmsI GCATC 6 cut(s) 394, 472, 950, 1058, 1154, 1469
BpiI GAAGAC 1 cut(s) 1063
BpmI CTGGAG 1 cut(s) 1065
Bpu10I CCTNAGC 1 cut(s) 1581
Bpu14I TTCGAA 3 cut(s) 419, 1471, 1761
BpuEI CTTGAG 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 1518
BsaAI YACGTR 1 cut(s) 151
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 1100
BsaJI CCNNGG 3 cut(s) 273, 686, 1635
BsaWI WCCGGW 3 cut(s) 176, 226, 1148
Bsc4I CCNNNNNNNGG 4 cut(s) 274, 647, 1048, 1523
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 2 cut(s) 561, 596
Bse3DI GCAATG 2 cut(s) 382, 912
BseDI CCNNGG 3 cut(s) 273, 686, 1635
BseGI GGATG 3 cut(s) 965, 1145, 1256
BseLI CCNNNNNNNGG 4 cut(s) 274, 647, 1048, 1523
BseMI GCAATG 2 cut(s) 382, 912
BseMII CTCAG 4 cut(s) 318, 803, 1430, 1647
BseNI ACTGG 2 cut(s) 561, 596
BseRI GAGGAG 3 cut(s) 203, 262, 1253
BseSI GKGCMC 1 cut(s) 729
BseXI GCAGC 8 cut(s) 38, 67, 187, 384, 506, 1264, 1534, 1689
BshFI GGCC 1 cut(s) 1247
BshNI GGYRCC 2 cut(s) 724, 1180
BsiSI CCGG 6 cut(s) 8, 177, 227, 938, 1149, 1517
BslFI GGGAC 1 cut(s) 1380
BslI CCNNNNNNNGG 4 cut(s) 274, 647, 1048, 1523
BsmAI GTCTC 7 cut(s) 24, 143, 228, 1100, 1166, 1214, 1419
BsmBI CGTCTC 3 cut(s) 24, 143, 228
BsmFI GGGAC 1 cut(s) 1380
BsmI GAATGC 1 cut(s) 514
BsnI GGCC 1 cut(s) 1247
Bso31I GGTCTC 1 cut(s) 1100
Bsp119I TTCGAA 3 cut(s) 419, 1471, 1761
Bsp1286I GDGCHC 2 cut(s) 668, 729
Bsp143I GATC 1 cut(s) 1477
BspANI GGCC 1 cut(s) 1247
BspCNI CTCAG 4 cut(s) 317, 804, 1429, 1648
BspLI GGNNCC 3 cut(s) 726, 1182, 1248
BspT104I TTCGAA 3 cut(s) 419, 1471, 1761
BspT107I GGYRCC 2 cut(s) 724, 1180
BspTNI GGTCTC 1 cut(s) 1100
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 2 cut(s) 382, 912
BsrFI RCCGGY 1 cut(s) 7
BsrI ACTGG 2 cut(s) 561, 596
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 3 cut(s) 273, 686, 1635
BssMI GATC 1 cut(s) 1477
BssNI GRCGYC 1 cut(s) 96
Bst4CI ACNGT 3 cut(s) 44, 1413, 1607
Bst6I CTCTTC 4 cut(s) 20, 1164, 1270, 1758
BstACI GRCGYC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 151
BstBI TTCGAA 3 cut(s) 419, 1471, 1761
BstC8I GCNNGC 6 cut(s) 185, 747, 948, 1036, 1073, 1552
BstDEI CTNAG 7 cut(s) 304, 812, 1103, 1404, 1416, 1581, 1656
BstEII GGTNACC 1 cut(s) 44
BstF5I GGATG 3 cut(s) 965, 1145, 1256
BstHHI GCGC 2 cut(s) 170, 1282
BstKTI GATC 1 cut(s) 1480
BstMAI GTCTC 7 cut(s) 24, 143, 228, 1100, 1166, 1214, 1419
BstMBI GATC 1 cut(s) 1477
BstMWI GCNNNNNNNGC 4 cut(s) 141, 491, 943, 1560
BstNSI RCATGY 2 cut(s) 749, 1034
BstPI GGTNACC 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 1516
BstSFI CTRYAG 1 cut(s) 1689
BstSLI GKGCMC 1 cut(s) 729
BstV1I GCAGC 8 cut(s) 38, 67, 187, 384, 506, 1264, 1534, 1689
BstV2I GAAGAC 1 cut(s) 1063
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 1 cut(s) 1247
BtgZI GCGATG 2 cut(s) 26, 1314
BtrI CACGTC 1 cut(s) 1124
BtsCI GGATG 3 cut(s) 965, 1145, 1256
BtsIMutI CAGTG 1 cut(s) 1612
Cac8I GCNNGC 6 cut(s) 185, 747, 948, 1036, 1073, 1552
CaiI CAGNNNCTG 1 cut(s) 485
CfoI GCGC 2 cut(s) 170, 1282
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 3 cut(s) 535, 1246, 1724
CseI GACGC 4 cut(s) 41, 43, 85, 251
Csp6I GTAC 3 cut(s) 278, 342, 1052
CspCI CAANNNNNGTGG 2 cut(s) 1588, 1623
CviAII CATG 8 cut(s) 676, 746, 1031, 1061, 1099, 1339, 1441, 1446
CviQI GTAC 3 cut(s) 278, 342, 1052
DdeI CTNAG 7 cut(s) 304, 812, 1103, 1404, 1416, 1581, 1656
DpnI GATC 1 cut(s) 1479
DpnII GATC 1 cut(s) 1477
DraIII CACNNNGTG 1 cut(s) 151
Eam1104I CTCTTC 4 cut(s) 20, 1164, 1270, 1758
EarI CTCTTC 4 cut(s) 20, 1164, 1270, 1758
EciI GGCGGA 2 cut(s) 122, 131
Eco24I GRGCYC 1 cut(s) 668
Eco31I GGTCTC 1 cut(s) 1100
Eco47I GGWCC 2 cut(s) 535, 1724
Eco72I CACGTG 1 cut(s) 151
Eco91I GGTNACC 1 cut(s) 44
EcoO65I GGTNACC 1 cut(s) 44
EcoRI GAATTC 2 cut(s) 349, 1757
EcoT38I GRGCYC 1 cut(s) 668
Esp3I CGTCTC 3 cut(s) 24, 143, 228
FaeI CATG 8 cut(s) 679, 749, 1034, 1064, 1102, 1342, 1444, 1449
FaqI GGGAC 1 cut(s) 1380
FatI CATG 8 cut(s) 675, 745, 1030, 1060, 1098, 1338, 1440, 1445
FauI CCCGC 3 cut(s) 165, 1080, 1552
FblI GTMKAC 1 cut(s) 588
FokI GGATG 3 cut(s) 972, 1132, 1243
FriOI GRGCYC 1 cut(s) 668
FspBI CTAG 1 cut(s) 1707
GlaI GCGC 2 cut(s) 169, 1281
GsuI CTGGAG 1 cut(s) 1065
HaeIII GGCC 1 cut(s) 1247
HapII CCGG 6 cut(s) 8, 177, 227, 938, 1149, 1517
HgaI GACGC 4 cut(s) 41, 43, 85, 251
HhaI GCGC 2 cut(s) 170, 1282
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 8 cut(s) 679, 749, 1034, 1064, 1102, 1342, 1444, 1449
Hin6I GCGC 2 cut(s) 168, 1280
HinP1I GCGC 2 cut(s) 168, 1280
HinfI GANTC 8 cut(s) 295, 421, 557, 715, 794, 1222, 1468, 1488
HpaII CCGG 6 cut(s) 8, 177, 227, 938, 1149, 1517
HphI GGTGA 7 cut(s) 38, 67, 239, 257, 290, 1163, 1178
Hpy166II GTNNAC 2 cut(s) 589, 1132
Hpy188III TCNNGA 5 cut(s) 329, 505, 957, 1082, 1269
Hpy8I GTNNAC 2 cut(s) 589, 1132
Hpy99I CGWCG 2 cut(s) 71, 101
HpyAV CCTTC 5 cut(s) 214, 547, 1270, 1356, 1515
HpyCH4III ACNGT 3 cut(s) 44, 1413, 1607
HpyCH4IV ACGT 4 cut(s) 69, 150, 585, 1123
HpyCH4V TGCA 9 cut(s) 235, 372, 395, 494, 701, 922, 1034, 1460, 1554
HpyF10VI GCNNNNNNNGC 4 cut(s) 141, 491, 943, 1560
HpyF3I CTNAG 7 cut(s) 304, 812, 1103, 1404, 1416, 1581, 1656
HpySE526I ACGT 4 cut(s) 69, 150, 585, 1123
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 8 cut(s) 679, 749, 1034, 1064, 1102, 1342, 1444, 1449
HspAI GCGC 2 cut(s) 168, 1280
Kzo9I GATC 1 cut(s) 1477
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 8 cut(s) 38, 67, 187, 384, 506, 1264, 1534, 1689
LweI GCATC 6 cut(s) 394, 472, 950, 1058, 1154, 1469
MaeI CTAG 1 cut(s) 1707
MaeII ACGT 4 cut(s) 69, 150, 585, 1123
MaeIII GTNAC 8 cut(s) 3, 44, 263, 425, 563, 1318, 1376, 1630
MalI GATC 1 cut(s) 1479
MbiI CCGCTC 1 cut(s) 312
MboI GATC 1 cut(s) 1477
MhlI GDGCHC 2 cut(s) 668, 729
MluCI AATT 9 cut(s) 349, 764, 799, 826, 842, 1454, 1482, 1674, 1757
MlyI GAGTC 1 cut(s) 1216
MmeI TCCRAC 3 cut(s) 665, 771, 1770
MroXI GAANNNNTTC 1 cut(s) 1717
MseI TTAA 5 cut(s) 63, 621, 735, 1229, 1740
MslI CAYNNNNRTG 1 cut(s) 1137
MspA1I CMGCKG 3 cut(s) 51, 80, 1547
MspI CCGG 6 cut(s) 8, 177, 227, 938, 1149, 1517
MspR9I CCNGG 1 cut(s) 1518
Mva1269I GAATGC 1 cut(s) 514
MwoI GCNNNNNNNGC 4 cut(s) 141, 491, 943, 1560
NciI CCSGG 1 cut(s) 1518
NdeII GATC 1 cut(s) 1477
NlaIII CATG 8 cut(s) 679, 749, 1034, 1064, 1102, 1342, 1444, 1449
NlaIV GGNNCC 3 cut(s) 726, 1182, 1248
NmuCI GTSAC 5 cut(s) 3, 44, 263, 1318, 1376
NspI RCATGY 2 cut(s) 749, 1034
NspV TTCGAA 3 cut(s) 419, 1471, 1761
PaeI GCATGC 1 cut(s) 749
PctI GAATGC 1 cut(s) 514
PdmI GAANNNNTTC 1 cut(s) 1717
PfeI GAWTC 7 cut(s) 295, 421, 557, 715, 794, 1468, 1488
PleI GAGTC 1 cut(s) 1216
PmaCI CACGTG 1 cut(s) 151
PmlI CACGTG 1 cut(s) 151
PpsI GAGTC 1 cut(s) 1216
Ppu21I YACGTR 1 cut(s) 151
PspCI CACGTG 1 cut(s) 151
PspEI GGTNACC 1 cut(s) 44
PspN4I GGNNCC 3 cut(s) 726, 1182, 1248
PspPI GGNCC 3 cut(s) 535, 1246, 1724
PstNI CAGNNNCTG 1 cut(s) 485
RsaI GTAC 3 cut(s) 279, 343, 1053
RsaNI GTAC 3 cut(s) 278, 342, 1052
RseI CAYNNNNRTG 1 cut(s) 1137
SaqAI TTAA 5 cut(s) 63, 621, 735, 1229, 1740
Sau3AI GATC 1 cut(s) 1477
Sau96I GGNCC 3 cut(s) 535, 1246, 1724
SchI GAGTC 1 cut(s) 1216
ScrFI CCNGG 1 cut(s) 1518
SduI GDGCHC 2 cut(s) 668, 729
SfaNI GCATC 6 cut(s) 394, 472, 950, 1058, 1154, 1469
SfcI CTRYAG 1 cut(s) 1689
SfuI TTCGAA 3 cut(s) 419, 1471, 1761
SgrAI CRCCGGYG 1 cut(s) 7
SinI GGWCC 2 cut(s) 535, 1724
SmiMI CAYNNNNRTG 1 cut(s) 1137
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SphI GCATGC 1 cut(s) 749
Sse9I AATT 9 cut(s) 349, 764, 799, 826, 842, 1454, 1482, 1674, 1757
SspMI CTAG 1 cut(s) 1707
StyD4I CCNGG 1 cut(s) 1516
TaaI ACNGT 3 cut(s) 44, 1413, 1607
TaiI ACGT 4 cut(s) 72, 153, 588, 1126
TaqI TCGA 7 cut(s) 328, 419, 1270, 1360, 1463, 1471, 1761
TasI AATT 9 cut(s) 349, 764, 799, 826, 842, 1454, 1482, 1674, 1757
TatI WGTACW 1 cut(s) 341
TauI GCSGC 3 cut(s) 86, 96, 383
TfiI GAWTC 7 cut(s) 295, 421, 557, 715, 794, 1468, 1488
Tru1I TTAA 5 cut(s) 63, 621, 735, 1229, 1740
Tru9I TTAA 5 cut(s) 63, 621, 735, 1229, 1740
TscAI CASTG 1 cut(s) 1612
TseFI GTSAC 5 cut(s) 3, 44, 263, 1318, 1376
TseI GCWGC 8 cut(s) 51, 80, 200, 372, 494, 1277, 1547, 1702
Tsp45I GTSAC 5 cut(s) 3, 44, 263, 1318, 1376
TspDTI ATGAA 7 cut(s) 29, 588, 901, 1245, 1317, 1414, 1610
TspGWI ACGGA 2 cut(s) 210, 1382
TspRI CASTG 1 cut(s) 1612
VpaK11BI GGWCC 2 cut(s) 535, 1724
XapI RAATTY 6 cut(s) 349, 764, 799, 842, 1454, 1757
XceI RCATGY 2 cut(s) 749, 1034
XmiI GTMKAC 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 1717
XspI CTAG 1 cut(s) 1707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.