pycom05g18100

Pleckstrin homology domain-containing family A member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012657
Physical Location & Seq
Reverse (-)
21121426 .. 21122560
1135 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g18100.1

Sequence Viewer

Length: 573 bp
ATGAATCATGTTAAATCCGAATCCGGCGAGATGCTTGCCAAGCCTTTCTTGGATGTGTGCAAACACTTGCTGCCTGTTTTAGACAAGTTTGGAGCCGCCTTTACCCCTGTGAAATCTGATATTGGTAATAACATATCGAGGTTGGATTCTAAGTATTCATCGAGCCCGTCAGAATTTAACCTCTTGTATAGCATGGTTCGAGTTGAGATTGAAGCGAAAACTGCGAAATCCTCATCTAGTTGCACTAATGCTCTGCTCTGGCTAACAAGGGCTATGGATTACTTGGTGGAGCTGTTTCGGAATCTACATGATAATCCAGACTGGCCAATGTCGAAGGCTTGTAGCGAAGCGTACGCCAAGACGCTGAAGAAATCGCATAACTGGCTAGCTAGCTCAAGTTTTAGTGTTGGAATTAAGCTTGCTCCAGAGAGGACGAAATTTATGGACATATTAGGTGGTGAAACTCCTGAATTAAACTCTGATATGGAGAAATTCTGCACGAACTTCTCTCCACTCCTCGAAGAGAATCACAAGTTCTTGGACTCTGTTGGAATGGACGATTTGAAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GLTP

Protein Analysis

191

Amino Acids

21.28

Weight (kDa)

5.96

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GLTP PF08718 13 - 152 2.7e-44 Glycolipid transfer protein (GLTP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 96
AcoI YGGCCR 1 cut(s) 323
AcsI RAATTY 3 cut(s) 173, 437, 491
AcuI CTGAAG 1 cut(s) 386
AfaI GTAC 1 cut(s) 353
AgsI TTSAA 2 cut(s) 212, 565
AluBI AGCT 5 cut(s) 292, 389, 393, 418, 569
AluI AGCT 5 cut(s) 292, 389, 393, 418, 569
AoxI GGCC 1 cut(s) 323
ApeKI GCWGC 1 cut(s) 70
ApoI RAATTY 3 cut(s) 173, 437, 491
AsuHPI GGTGA 1 cut(s) 470
AsuNHI GCTAGC 2 cut(s) 385, 389
BalI TGGCCA 1 cut(s) 325
BanII GRGCYC 1 cut(s) 167
BbvI GCAGC 1 cut(s) 57
BcgI CGANNNNNNTGC 2 cut(s) 17, 51
BfaI CTAG 3 cut(s) 237, 386, 390
BisI GCNGC 2 cut(s) 71, 96
BlsI GCNGC 2 cut(s) 72, 97
BmiI GGNNCC 1 cut(s) 94
BmsI GCATC 1 cut(s) 21
BmtI GCTAGC 2 cut(s) 389, 393
BpmI CTGGAG 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 379
Bse1I ACTGG 2 cut(s) 326, 386
BseGI GGATG 1 cut(s) 58
BseNI ACTGG 2 cut(s) 326, 386
BseRI GAGGAG 1 cut(s) 506
BseXI GCAGC 1 cut(s) 57
BsgI GTGCAG 1 cut(s) 481
BshFI GGCC 1 cut(s) 325
BsiSI CCGG 1 cut(s) 24
BsiWI CGTACG 1 cut(s) 351
BsnI GGCC 1 cut(s) 325
Bsp1286I GDGCHC 1 cut(s) 167
BspACI CCGC 1 cut(s) 96
BspANI GGCC 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 94
BspOI GCTAGC 2 cut(s) 389, 393
BsrI ACTGG 2 cut(s) 326, 386
Bst6I CTCTTC 1 cut(s) 516
BstC8I GCNNGC 4 cut(s) 36, 387, 391, 420
BstDEI CTNAG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 58
BstMWI GCNNNNNNNGC 3 cut(s) 40, 221, 382
BstV1I GCAGC 1 cut(s) 57
BsuRI GGCC 1 cut(s) 325
BtsCI GGATG 1 cut(s) 58
Cac8I GCNNGC 4 cut(s) 36, 387, 391, 420
CseI GACGC 1 cut(s) 370
Csp6I GTAC 1 cut(s) 352
CviAII CATG 3 cut(s) 8, 193, 308
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 1 cut(s) 150
EaeI YGGCCR 1 cut(s) 323
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Eco24I GRGCYC 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 386
EcoT38I GRGCYC 1 cut(s) 167
FaeI CATG 3 cut(s) 11, 196, 311
FalI AAGNNNNNCTT 2 cut(s) 32, 64
FatI CATG 3 cut(s) 7, 192, 307
Fnu4HI GCNGC 2 cut(s) 71, 96
FokI GGATG 1 cut(s) 65
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 2 cut(s) 71, 96
FspBI CTAG 3 cut(s) 237, 386, 390
GluI GCNGC 2 cut(s) 71, 96
GsuI CTGGAG 1 cut(s) 408
HaeIII GGCC 1 cut(s) 325
HapII CCGG 1 cut(s) 24
HgaI GACGC 1 cut(s) 370
Hin1II CATG 3 cut(s) 11, 196, 311
HindIII AAGCTT 2 cut(s) 416, 567
HinfI GANTC 6 cut(s) 4, 20, 146, 301, 526, 542
HpaII CCGG 1 cut(s) 24
HphI GGTGA 1 cut(s) 470
Hpy188I TCNGA 5 cut(s) 19, 118, 172, 300, 481
Hpy188III TCNNGA 3 cut(s) 317, 425, 467
HpyAV CCTTC 1 cut(s) 328
HpyCH4V TGCA 3 cut(s) 60, 243, 498
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 221, 382
HpyF3I CTNAG 1 cut(s) 150
Hsp92II CATG 3 cut(s) 11, 196, 311
LmnI GCTCC 3 cut(s) 92, 289, 427
LpnPI CCDG 9 cut(s) 37, 87, 120, 244, 307, 330, 367, 438, 480
Lsp1109I GCAGC 1 cut(s) 57
LweI GCATC 1 cut(s) 21
MaeI CTAG 3 cut(s) 237, 386, 390
MboII GAAGA 2 cut(s) 379, 533
MhlI GDGCHC 1 cut(s) 167
MlsI TGGCCA 1 cut(s) 325
MluCI AATT 5 cut(s) 173, 411, 437, 470, 491
MluNI TGGCCA 1 cut(s) 325
MlyI GAGTC 1 cut(s) 536
MmeI TCCRAC 3 cut(s) 123, 388, 529
MnlI CCTC 5 cut(s) 132, 191, 241, 423, 527
Mox20I TGGCCA 1 cut(s) 325
MscI TGGCCA 1 cut(s) 325
MseI TTAA 4 cut(s) 12, 177, 414, 473
Msp20I TGGCCA 1 cut(s) 325
MspI CCGG 1 cut(s) 24
MwoI GCNNNNNNNGC 3 cut(s) 40, 221, 382
NheI GCTAGC 2 cut(s) 385, 389
NlaIII CATG 3 cut(s) 11, 196, 311
NlaIV GGNNCC 1 cut(s) 94
PfeI GAWTC 5 cut(s) 4, 20, 146, 301, 526
Pfl23II CGTACG 1 cut(s) 351
PkrI GCNGC 2 cut(s) 72, 97
PleI GAGTC 1 cut(s) 536
PpsI GAGTC 1 cut(s) 536
PspLI CGTACG 1 cut(s) 351
PspN4I GGNNCC 1 cut(s) 94
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
SaqAI TTAA 4 cut(s) 12, 177, 414, 473
SatI GCNGC 2 cut(s) 71, 96
SchI GAGTC 1 cut(s) 536
SduI GDGCHC 1 cut(s) 167
SetI ASST 8 cut(s) 143, 183, 294, 391, 395, 420, 457, 571
SfaNI GCATC 1 cut(s) 21
SmlI CTYRAG 1 cut(s) 394
SmoI CTYRAG 1 cut(s) 394
Sse9I AATT 5 cut(s) 173, 411, 437, 470, 491
SsiI CCGC 1 cut(s) 96
SspMI CTAG 3 cut(s) 237, 386, 390
TaqI TCGA 5 cut(s) 137, 161, 199, 332, 519
TasI AATT 5 cut(s) 173, 411, 437, 470, 491
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 5 cut(s) 4, 20, 146, 301, 526
Tru1I TTAA 4 cut(s) 12, 177, 414, 473
Tru9I TTAA 4 cut(s) 12, 177, 414, 473
TseI GCWGC 1 cut(s) 70
TspDTI ATGAA 2 cut(s) 17, 147
XapI RAATTY 3 cut(s) 173, 437, 491
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XspI CTAG 3 cut(s) 237, 386, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.