pycom05g24020

polyamine transporter At3g13620

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011363
Physical Location & Seq
Forward (+)
25611607 .. 25612711
1105 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 975 bp
ATGTATGTAACTTTAATTTTTTTAGCACCTTCCTTATCACCTTTTCTCCTTAATCTTTCACTTTTATATTTTCCCTTTTTTACTCTTTCAATCAGTGGCAGTCAAAATGCATATTTTAAACCTGCAGATGGCATTGAAATTTGTGTGTGGCAGCAAGTTGCAATCTCTGTTTCTACTCTGATTATATCTTTCGTAAACTATACTGGTTTAACTATTGTTGGATATGCTGCTGTTCTTCTCGCTGTCGCTTCCCTTTCGCCTTTTATTTTGATGTGTTTGATTGCCATTCCGAAGATTCATCCTCACCGTTGGTTGAGTCTCGGGCGGAAGGGTGTTGAGAAAGATTGGAACTTGTTCTTCAACACCCTCTTTTGGAATTTAAATTTTTGGGACAATGTGAGTACTTTAGCTGGGGAAGTAGATAACCCCCAAAAAACTTTTCCGGTGGCTCTTTTCGTGGCAGTGATCTTTACTTGCTTGGCGTACTTGTTCCCGCTTTTTGCTGTTATTGGTTCGGTTAATATGGATCAAACTAGATGGGGTTCTGGATTTCATGCAGATGCAGTCAAAATGCATATTTTAAACCTGCAGATGGCAGTCAAAATGCATAAGATTTTGTTTAAATTTGTGTGTGGCAGCAAGTTGAAATTTGTCAGCATATTCTTTGTAACATCCCGTCCCAAATTTCATTTTAACGTACGTGTGAATTTACGATTTTACCCCTTAATTCGTTTTTATCACATTTGTGTTGTGTGGTGTGGTGTTGTAGGACCACACACACTCATACACTCTCTTTTCTCCAGGGATTCCCTCCCTCTTTCCCTCACTTTCTGTCACTCTGTCTCTCTCTCTCTCTCTCTCCCCGAGTCCATCTTCTTCTTCTTCCTTCTGCAAACACACGGACTACACACAAACACACTCAAAACTTTCACCAATCAAGAAACCAATTACACCATTGAACTCGTGAGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0042802
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AA_permease_2

Protein Analysis

325

Amino Acids

36.86

Weight (kDa)

9.27

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_permease_2 PF13520 19 - 185 1.9e-09 Amino acid permease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 130, 594
AciI CCGC 2 cut(s) 325, 494
AclWI GGATC 1 cut(s) 534
AcsI RAATTY 7 cut(s) 138, 376, 382, 623, 647, 683, 706
AfaI GTAC 3 cut(s) 403, 485, 699
AfiI CCNNNNNNNGG 3 cut(s) 128, 372, 592
AflIII ACRYGT 1 cut(s) 700
AgsI TTSAA 5 cut(s) 90, 137, 361, 646, 959
AjnI CCWGG 1 cut(s) 800
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
AleI CACNNNNGTG 1 cut(s) 744
AluBI AGCT 1 cut(s) 410
AluI AGCT 1 cut(s) 410
Alw26I GTCTC 2 cut(s) 323, 847
AlwI GGATC 1 cut(s) 534
Ama87I CYCGRG 2 cut(s) 320, 863
ApeKI GCWGC 3 cut(s) 151, 227, 636
ApoI RAATTY 7 cut(s) 138, 376, 382, 623, 647, 683, 706
Asp700I GAANNNNTTC 1 cut(s) 353
AspS9I GGNCC 1 cut(s) 770
AsuHPI GGTGA 3 cut(s) 30, 296, 922
AvaI CYCGRG 2 cut(s) 320, 863
AvaII GGWCC 1 cut(s) 770
BauI CACGAG 1 cut(s) 962
BbvI GCAGC 3 cut(s) 163, 214, 648
BccI CCATC 4 cut(s) 122, 531, 586, 878
BciT130I CCWGG 1 cut(s) 802
BcoDI GTCTC 2 cut(s) 323, 847
BfaI CTAG 1 cut(s) 534
BfmI CTRYAG 2 cut(s) 123, 587
BfuAI ACCTGC 2 cut(s) 130, 594
BisI GCNGC 3 cut(s) 152, 228, 637
BlsI GCNGC 3 cut(s) 153, 229, 638
BmcAI AGTACT 1 cut(s) 403
Bme1390I CCNGG 1 cut(s) 802
Bme18I GGWCC 1 cut(s) 770
BmeT110I CYCGRG 2 cut(s) 320, 863
BmgT120I GGNCC 1 cut(s) 770
BmrFI CCNGG 1 cut(s) 802
BmsI GCATC 1 cut(s) 550
BpmI CTGGAG 1 cut(s) 784
BsaAI YACGTR 1 cut(s) 701
BsaJI CCNNGG 1 cut(s) 801
BsaWI WCCGGW 1 cut(s) 442
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 372, 592
Bse1I ACTGG 1 cut(s) 208
BseBI CCWGG 1 cut(s) 802
BseDI CCNNGG 1 cut(s) 801
BseGI GGATG 2 cut(s) 298, 671
BseLI CCNNNNNNNGG 3 cut(s) 128, 372, 592
BseNI ACTGG 1 cut(s) 208
BseXI GCAGC 3 cut(s) 163, 214, 648
BseYI CCCAGC 1 cut(s) 410
BsiHKCI CYCGRG 2 cut(s) 320, 863
BsiSI CCGG 1 cut(s) 443
BsiWI CGTACG 1 cut(s) 697
BslFI GGGAC 2 cut(s) 404, 663
BslI CCNNNNNNNGG 3 cut(s) 128, 372, 592
BsmAI GTCTC 2 cut(s) 323, 847
BsmFI GGGAC 2 cut(s) 404, 663
BsoBI CYCGRG 2 cut(s) 320, 863
Bsp143I GATC 2 cut(s) 465, 526
BspACI CCGC 2 cut(s) 325, 494
BspMAI CTGCAG 2 cut(s) 127, 591
BspMI ACCTGC 2 cut(s) 130, 594
BspPI GGATC 1 cut(s) 534
BsrI ACTGG 1 cut(s) 208
BssECI CCNNGG 1 cut(s) 801
BssMI GATC 2 cut(s) 465, 526
BssSI CACGAG 1 cut(s) 962
Bst2BI CACGAG 1 cut(s) 962
Bst2UI CCWGG 1 cut(s) 802
Bst4CI ACNGT 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 701
BstF5I GGATG 2 cut(s) 298, 671
BstKTI GATC 2 cut(s) 468, 529
BstMAI GTCTC 2 cut(s) 323, 847
BstMBI GATC 2 cut(s) 465, 526
BstNI CCWGG 1 cut(s) 802
BstSCI CCNGG 1 cut(s) 800
BstSFI CTRYAG 2 cut(s) 123, 587
BstV1I GCAGC 3 cut(s) 163, 214, 648
BtsCI GGATG 2 cut(s) 298, 671
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 2 cut(s) 100, 468
BveI ACCTGC 2 cut(s) 130, 594
Cfr13I GGNCC 1 cut(s) 770
Csp6I GTAC 3 cut(s) 402, 484, 698
CviAII CATG 1 cut(s) 554
CviJI RGCY 2 cut(s) 410, 449
CviKI_1 RGCY 2 cut(s) 410, 449
CviQI GTAC 3 cut(s) 402, 484, 698
DpnI GATC 2 cut(s) 467, 528
DpnII GATC 2 cut(s) 465, 526
DraI TTTAAA 4 cut(s) 118, 381, 582, 622
EciI GGCGGA 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 770
Eco88I CYCGRG 2 cut(s) 320, 863
EcoRII CCWGG 1 cut(s) 800
EcoT22I ATGCAT 3 cut(s) 112, 576, 609
FaeI CATG 1 cut(s) 557
FaqI GGGAC 2 cut(s) 404, 663
FatI CATG 1 cut(s) 553
FauI CCCGC 1 cut(s) 501
Fnu4HI GCNGC 3 cut(s) 152, 228, 637
FokI GGATG 2 cut(s) 285, 658
Fsp4HI GCNGC 3 cut(s) 152, 228, 637
FspBI CTAG 1 cut(s) 534
GluI GCNGC 3 cut(s) 152, 228, 637
GsaI CCCAGC 1 cut(s) 414
GsuI CTGGAG 1 cut(s) 784
HapII CCGG 1 cut(s) 443
Hin1II CATG 1 cut(s) 557
HinfI GANTC 4 cut(s) 295, 316, 806, 866
HpaII CCGG 1 cut(s) 443
HphI GGTGA 3 cut(s) 30, 296, 922
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 2 cut(s) 180, 291
Hpy188III TCNNGA 3 cut(s) 546, 938, 964
Hpy8I GTNNAC 1 cut(s) 196
HpyAV CCTTC 3 cut(s) 39, 322, 896
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4IV ACGT 2 cut(s) 696, 700
HpyCH4V TGCA 9 cut(s) 110, 125, 161, 557, 563, 574, 589, 607, 892
HpySE526I ACGT 2 cut(s) 696, 700
Hsp92II CATG 1 cut(s) 557
Kzo9I GATC 2 cut(s) 465, 526
LpnPI CCDG 8 cut(s) 135, 189, 396, 456, 531, 599, 787, 814
Lsp1109I GCAGC 3 cut(s) 163, 214, 648
LweI GCATC 1 cut(s) 550
MaeI CTAG 1 cut(s) 534
MaeII ACGT 2 cut(s) 696, 700
MaeIII GTNAC 3 cut(s) 7, 667, 833
MalI GATC 2 cut(s) 467, 528
MboI GATC 2 cut(s) 465, 526
MboII GAAGA 7 cut(s) 227, 304, 349, 865, 868, 871, 874
MlyI GAGTC 2 cut(s) 325, 875
MmeI TCCRAC 1 cut(s) 199
MnlI CCTC 6 cut(s) 312, 377, 821, 825, 833, 960
Mph1103I ATGCAT 3 cut(s) 112, 576, 609
MroXI GAANNNNTTC 1 cut(s) 353
MslI CAYNNNNRTG 2 cut(s) 558, 744
MspI CCGG 1 cut(s) 443
MspR9I CCNGG 1 cut(s) 802
MvaI CCWGG 1 cut(s) 802
NdeII GATC 2 cut(s) 465, 526
NlaIII CATG 1 cut(s) 557
NmuCI GTSAC 1 cut(s) 833
NsiI ATGCAT 3 cut(s) 112, 576, 609
OliI CACNNNNGTG 1 cut(s) 744
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 2 cut(s) 295, 806
Pfl23II CGTACG 1 cut(s) 697
PkrI GCNGC 3 cut(s) 153, 229, 638
PleI GAGTC 2 cut(s) 324, 874
PpsI GAGTC 2 cut(s) 324, 874
Ppu21I YACGTR 1 cut(s) 701
Psp6I CCWGG 1 cut(s) 800
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 1 cut(s) 800
PspLI CGTACG 1 cut(s) 697
PspPI GGNCC 1 cut(s) 770
PstI CTGCAG 2 cut(s) 127, 591
RsaI GTAC 3 cut(s) 403, 485, 699
RsaNI GTAC 3 cut(s) 402, 484, 698
RseI CAYNNNNRTG 2 cut(s) 558, 744
SatI GCNGC 3 cut(s) 152, 228, 637
Sau3AI GATC 2 cut(s) 465, 526
Sau96I GGNCC 1 cut(s) 770
ScaI AGTACT 1 cut(s) 403
SchI GAGTC 2 cut(s) 325, 875
ScrFI CCNGG 1 cut(s) 802
SetI ASST 8 cut(s) 31, 43, 124, 412, 588, 699, 703, 971
SfaNI GCATC 1 cut(s) 550
SfcI CTRYAG 2 cut(s) 123, 587
SinI GGWCC 1 cut(s) 770
SmiI ATTTAAAT 1 cut(s) 381
SmiMI CAYNNNNRTG 2 cut(s) 558, 744
SsiI CCGC 2 cut(s) 325, 494
SspMI CTAG 1 cut(s) 534
StyD4I CCNGG 1 cut(s) 800
SwaI ATTTAAAT 1 cut(s) 381
TaaI ACNGT 1 cut(s) 308
TaiI ACGT 2 cut(s) 699, 703
TatI WGTACW 1 cut(s) 401
TfiI GAWTC 2 cut(s) 295, 806
TscAI CASTG 2 cut(s) 100, 468
TseFI GTSAC 1 cut(s) 833
TseI GCWGC 3 cut(s) 151, 227, 636
Tsp45I GTSAC 1 cut(s) 833
TspDTI ATGAA 3 cut(s) 287, 542, 677
TspGWI ACGGA 1 cut(s) 915
TspRI CASTG 2 cut(s) 100, 468
VpaK11BI GGWCC 1 cut(s) 770
XapI RAATTY 7 cut(s) 138, 376, 382, 623, 647, 683, 706
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 1 cut(s) 534
ZrmI AGTACT 1 cut(s) 403
Zsp2I ATGCAT 3 cut(s) 112, 576, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.