pycom05g25940

hmr,ptac12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010065
Physical Location & Seq
Forward (+)
27220752 .. 27222208
1457 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25940.3

Sequence Viewer

Length: 687 bp
ATGGCATCTCTACCAGGAACTTGGCTGTATCAAGATAGAGGATTGATAGGTACAGTCTCATCACAGAGCCATGCTTCTGGGAACCCATGTTTCAATCATTTCAAGGCATCAACTGTAAGTTCATTTCCAGTTGGATTATTCCAAGTTAGAGTTGGCTTGAATCTATTACCACAGAGGGCACCTTTACTTCCTTGTATAAAGTGTGAGAAGGATGATGCGTCCTCCAAAGAGGTCTCTATTGAGCGTCCACCCTACGATAGTTATATGGACTCAACATCTGGGCAGCTCGAACCTGCATCTGGTGCTTATGCCAGCATTCCAGGGCAGGATTATTGGCCTGAAGGCACAGCTAGTCGAGTTAGAGCTGCCGGGGCTCCTGAGCCCACTGGTAAATCAGTTGGATCACCATCTTATGGTAAAAATCCTGGAAGTAGGAGGAGGAAGAATAAAACATCAGTTGCTGCCGGTGAATCTATTGAACCAAGTGTAGAGTCAAGTGATACAGTGGCAGTGGAACTGCTGGAACCAGAAACTTCAGAGGACACACTAGAGGATCCTAAAGATTCCCCATCTGAATATGTTGTCTATCAGACTGAAGCTGAAAAAGAAGAAGAAACAGGATTTGACCGGACAAGAAACTCGGACGTCCCCATCCCTTTATTGATCCAGAAACAAAGAAGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0003727 GO:0005488 GO:0008150 GO:0010468 GO:0019222 GO:0050789 GO:0060255 GO:0065007 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

229

Amino Acids

24.64

Weight (kDa)

4.94

Isoelectric Point (pI)

59.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 648
Acc36I ACCTGC 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 178
AccB7I CCANNNNNTGG 1 cut(s) 413
AclWI GGATC 4 cut(s) 409, 548, 561, 658
AcuI CTGAAG 3 cut(s) 360, 519, 615
AcyI GRCGYC 1 cut(s) 645
AfaI GTAC 1 cut(s) 52
AfiI CCNNNNNNNGG 2 cut(s) 299, 413
AgsI TTSAA 4 cut(s) 94, 103, 160, 479
AjnI CCWGG 3 cut(s) 13, 319, 424
AluBI AGCT 4 cut(s) 286, 350, 365, 599
AluI AGCT 4 cut(s) 286, 350, 365, 599
Alw26I GTCTC 2 cut(s) 61, 238
AlwI GGATC 4 cut(s) 409, 548, 561, 658
AlwNI CAGNNNCTG 1 cut(s) 461
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 3 cut(s) 283, 365, 461
AsuC2I CCSGG 1 cut(s) 370
AsuHPI GGTGA 2 cut(s) 396, 479
BaeGI GKGCMC 1 cut(s) 181
BamHI GGATCC 1 cut(s) 553
BanI GGYRCC 1 cut(s) 178
BanII GRGCYC 2 cut(s) 376, 384
BbvI GCAGC 3 cut(s) 295, 352, 448
BccI CCATC 3 cut(s) 415, 577, 659
BciT130I CCWGG 3 cut(s) 15, 321, 426
BcnI CCSGG 1 cut(s) 370
BcoDI GTCTC 2 cut(s) 61, 238
BfaI CTAG 2 cut(s) 351, 548
BfuAI ACCTGC 1 cut(s) 301
BisI GCNGC 3 cut(s) 284, 366, 462
BlsI GCNGC 3 cut(s) 285, 367, 463
Bme1390I CCNGG 4 cut(s) 15, 321, 370, 426
BmiI GGNNCC 5 cut(s) 83, 180, 375, 525, 555
BmrFI CCNGG 4 cut(s) 15, 321, 370, 426
BmsI GCATC 4 cut(s) 14, 116, 205, 305
Bpu10I CCTNAGC 1 cut(s) 378
BpuMI CCSGG 1 cut(s) 370
BsaBI GATNNNNATC 1 cut(s) 406
BsaHI GRCGYC 1 cut(s) 645
BsaI GGTCTC 1 cut(s) 238
BsaJI CCNNGG 2 cut(s) 320, 369
BsaWI WCCGGW 1 cut(s) 627
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 413
Bse118I RCCGGY 1 cut(s) 464
Bse1I ACTGG 2 cut(s) 128, 391
Bse8I GATNNNNATC 1 cut(s) 406
BseBI CCWGG 3 cut(s) 15, 321, 426
BseDI CCNNGG 2 cut(s) 320, 369
BseGI GGATG 2 cut(s) 217, 651
BseJI GATNNNNATC 1 cut(s) 406
BseLI CCNNNNNNNGG 2 cut(s) 299, 413
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 2 cut(s) 128, 391
BseRI GAGGAG 1 cut(s) 451
BseSI GKGCMC 1 cut(s) 181
BseXI GCAGC 3 cut(s) 295, 352, 448
BshFI GGCC 1 cut(s) 337
BshNI GGYRCC 1 cut(s) 178
BsiSI CCGG 3 cut(s) 369, 465, 628
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 2 cut(s) 299, 413
BsmAI GTCTC 2 cut(s) 61, 238
BsmFI GGGAC 1 cut(s) 632
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 1 cut(s) 337
Bso31I GGTCTC 1 cut(s) 238
Bsp1286I GDGCHC 3 cut(s) 181, 376, 384
Bsp143I GATC 3 cut(s) 401, 553, 663
BspANI GGCC 1 cut(s) 337
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 5 cut(s) 83, 180, 375, 525, 555
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 4 cut(s) 409, 548, 561, 658
BspT107I GGYRCC 1 cut(s) 178
BspTNI GGTCTC 1 cut(s) 238
BsrFI RCCGGY 1 cut(s) 464
BsrI ACTGG 2 cut(s) 128, 391
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 2 cut(s) 320, 369
BssMI GATC 3 cut(s) 401, 553, 663
BssNI GRCGYC 1 cut(s) 645
Bst2UI CCWGG 3 cut(s) 15, 321, 426
Bst4CI ACNGT 3 cut(s) 55, 115, 505
BstACI GRCGYC 1 cut(s) 645
BstAPI GCANNNNNTGC 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 1 cut(s) 378
BstF5I GGATG 2 cut(s) 217, 651
BstKTI GATC 3 cut(s) 404, 556, 666
BstMAI GTCTC 2 cut(s) 61, 238
BstMBI GATC 3 cut(s) 401, 553, 663
BstMWI GCNNNNNNNGC 2 cut(s) 302, 371
BstNI CCWGG 3 cut(s) 15, 321, 426
BstSCI CCNGG 4 cut(s) 13, 319, 368, 424
BstSLI GKGCMC 1 cut(s) 181
BstV1I GCAGC 3 cut(s) 295, 352, 448
BstX2I RGATCY 1 cut(s) 553
BstXI CCANNNNNNTGG 2 cut(s) 21, 77
BstYI RGATCY 1 cut(s) 553
BsuRI GGCC 1 cut(s) 337
BtsCI GGATG 2 cut(s) 217, 651
BtsI GCAGTG 1 cut(s) 516
BtsIMutI CAGTG 3 cut(s) 384, 510, 516
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 313
CaiI CAGNNNCTG 1 cut(s) 461
Cfr10I RCCGGY 1 cut(s) 464
CseI GACGC 2 cut(s) 207, 233
Csp6I GTAC 1 cut(s) 51
CviAII CATG 2 cut(s) 71, 87
CviQI GTAC 1 cut(s) 51
DdeI CTNAG 1 cut(s) 378
DpnI GATC 3 cut(s) 403, 555, 665
DpnII GATC 3 cut(s) 401, 553, 663
Eco24I GRGCYC 2 cut(s) 376, 384
Eco31I GGTCTC 1 cut(s) 238
Eco57I CTGAAG 3 cut(s) 360, 519, 615
EcoRII CCWGG 3 cut(s) 13, 319, 424
EcoT38I GRGCYC 2 cut(s) 376, 384
FaeI CATG 2 cut(s) 74, 90
FaiI YATR 8 cut(s) 72, 88, 197, 264, 266, 309, 414, 579
FaqI GGGAC 1 cut(s) 632
FatI CATG 2 cut(s) 70, 86
Fnu4HI GCNGC 3 cut(s) 284, 366, 462
FokI GGATG 2 cut(s) 224, 638
FriOI GRGCYC 2 cut(s) 376, 384
Fsp4HI GCNGC 3 cut(s) 284, 366, 462
FspBI CTAG 2 cut(s) 351, 548
GluI GCNGC 3 cut(s) 284, 366, 462
HaeIII GGCC 1 cut(s) 337
HapII CCGG 3 cut(s) 369, 465, 628
HgaI GACGC 2 cut(s) 207, 233
Hin1I GRCGYC 1 cut(s) 645
Hin1II CATG 2 cut(s) 74, 90
HinfI GANTC 5 cut(s) 160, 269, 470, 491, 563
HpaII CCGG 3 cut(s) 369, 465, 628
HphI GGTGA 2 cut(s) 396, 479
Hpy166II GTNNAC 1 cut(s) 248
Hpy188I TCNGA 4 cut(s) 538, 574, 591, 643
Hpy188III TCNNGA 3 cut(s) 32, 377, 667
Hpy8I GTNNAC 1 cut(s) 248
HpyAV CCTTC 2 cut(s) 202, 335
HpyCH4III ACNGT 3 cut(s) 55, 115, 505
HpyCH4IV ACGT 1 cut(s) 645
HpyCH4V TGCA 1 cut(s) 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 371
HpyF3I CTNAG 1 cut(s) 378
HpySE526I ACGT 1 cut(s) 645
Hsp92I GRCGYC 1 cut(s) 645
Hsp92II CATG 2 cut(s) 74, 90
Kzo9I GATC 3 cut(s) 401, 553, 663
LmnI GCTCC 1 cut(s) 379
Lsp1109I GCAGC 3 cut(s) 295, 352, 448
LweI GCATC 4 cut(s) 14, 116, 205, 305
MaeI CTAG 2 cut(s) 351, 548
MaeII ACGT 1 cut(s) 645
MalI GATC 3 cut(s) 403, 555, 665
MboI GATC 3 cut(s) 401, 553, 663
MboII GAAGA 3 cut(s) 454, 620, 623
MflI RGATCY 1 cut(s) 553
MhlI GDGCHC 3 cut(s) 181, 376, 384
MlyI GAGTC 2 cut(s) 263, 500
MmeI TCCRAC 2 cut(s) 112, 379
MnlI CCTC 8 cut(s) 32, 168, 223, 232, 429, 432, 532, 544
MspI CCGG 3 cut(s) 369, 465, 628
MspR9I CCNGG 4 cut(s) 15, 321, 370, 426
Mva1269I GAATGC 1 cut(s) 315
MvaI CCWGG 3 cut(s) 15, 321, 426
MwoI GCNNNNNNNGC 2 cut(s) 302, 371
NciI CCSGG 1 cut(s) 370
NdeII GATC 3 cut(s) 401, 553, 663
NlaIII CATG 2 cut(s) 74, 90
NlaIV GGNNCC 5 cut(s) 83, 180, 375, 525, 555
PctI GAATGC 1 cut(s) 315
PfeI GAWTC 3 cut(s) 160, 470, 563
PflMI CCANNNNNTGG 1 cut(s) 413
PfoI TCCNGGA 1 cut(s) 424
PkrI GCNGC 3 cut(s) 285, 367, 463
PleI GAGTC 2 cut(s) 263, 499
PpsI GAGTC 2 cut(s) 263, 499
Psp6I CCWGG 3 cut(s) 13, 319, 424
PspGI CCWGG 3 cut(s) 13, 319, 424
PspN4I GGNNCC 5 cut(s) 83, 180, 375, 525, 555
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 1 cut(s) 553
RsaI GTAC 1 cut(s) 52
RsaNI GTAC 1 cut(s) 51
SatI GCNGC 3 cut(s) 284, 366, 462
Sau3AI GATC 3 cut(s) 401, 553, 663
SchI GAGTC 2 cut(s) 263, 500
ScrFI CCNGG 4 cut(s) 15, 321, 370, 426
SduI GDGCHC 3 cut(s) 181, 376, 384
SetI ASST 9 cut(s) 52, 184, 234, 288, 295, 352, 367, 601, 648
SfaNI GCATC 4 cut(s) 14, 116, 205, 305
SspMI CTAG 2 cut(s) 351, 548
StyD4I CCNGG 4 cut(s) 13, 319, 368, 424
TaaI ACNGT 3 cut(s) 55, 115, 505
TaiI ACGT 1 cut(s) 648
TaqI TCGA 2 cut(s) 288, 355
TfiI GAWTC 3 cut(s) 160, 470, 563
TscAI CASTG 3 cut(s) 391, 510, 516
TseI GCWGC 3 cut(s) 283, 365, 461
TspDTI ATGAA 1 cut(s) 111
TspRI CASTG 3 cut(s) 391, 510, 516
Van91I CCANNNNNTGG 1 cut(s) 413
XcmI CCANNNNNNNNNTGG 1 cut(s) 149
XspI CTAG 2 cut(s) 351, 548
ZraI GACGTC 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.