RchiOBHm_Chr5g0014971

hmr,ptac12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010065
Physical Location & Seq
Reverse (-)
10362573 .. 10368120
5548 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29540

Sequence Viewer

Length: 1647 bp
ATGGCATCAATATCAACAACTTGGCTCTATCAAGATAGAGGATTGAAAGGTACGGTCTTTTCACAGAGCCATGCTGCTGGGAACCCATGTTTCAATTACAAGACATCGTTTGTTAGATCATTTCCAGTTAGAGTTGGCTTAAGTATATCGCCGAGGAGGGGACCTTTACTTCCTTGTATAAAGTGTGAGAAGAATGATGAATCTTCCAAAGATGTGTCTGCTGATGAATCTTCCAAAGATGTGTCTATTGATGAATCTCCAAAAGAGGTTACGAAAGAGGTGACTGTCGAGCGTGCACCCTACTATAGTTATTTGGACTCTACATCTGGGCAGCTAGAACCAGCATCTGGTGCTCGTGCCAGCATTCCCGGACAGGATTATTGGCCTGAAGGGACCGCTAGTCGAGTTAGGGCTGCCAATGCACCCGAGCCAACTGGTGAGTCTGTTGGCTCTCCATCTTTTGGTAGAAGTCCTGGAAGTAGGAGGAGGAAGAAGAAAGCATCAGTTGAGGCTCATGACTCTTCTGACCCGAGTGTAAAATCAAGTGAACTAGCGGCAGTGGCAATGGAACCAGAGATTTTGGAGGACACAATTGAAGACTTTAAAGATATCTCATCTGACTATGTGGTCTATCAGACGGGACCTGAAAAAGGAGAAGAAACAGGATATGAACTAGACAAGAAACTTGGACGCCCTCATCCATTTATTGATCCAGAAACTAAGAAGCCATTAGAAGAGCCGCTTACAAGTGACGAACTATGGTGGAACTGGAGAAAGCCAGACAAGGAACAATGGTCCAGATGGCAAAGGAGGCGACCCGACAGTGAAACGGTTTTTCTCAAAGCAATGGCAGAGACTGGGCAAGTAAAGCTTTATGGAGAAGAGCCAACATTGACTGAAACTTCACTTTACAGAGCAAGGCGACATCTTTTTAAGGAAGAAAGGCTTCAAGCTGAACAAGAGAAACTGGATAAGATAGGTCCACTTGCATATTATTCAGAATGGGTAAAAGATTGGAAGAGAGACACTTCTCGAGAAGCAGTTCAAAAGCATTTTGAAGAGACTGGTGAAAATGAAACAACCCAACTAATTGACATGTTTAGTCATCAAACAGATCGGGAGTATCGCATAATGATGGGGACTGATATCCGAATAAAGAGGGATCCTTTAGCAATGCGAATGCGGGAGGATCAAATAAAGCAAATATGGGGTGGAGATCCAGTGTACCCAACTGTTAACTATATTCAAGATCCAGATGAAGTGATTGATTACAGAGGCCCTGATTTTCATGAACCAACACCAAATATGCTGTCTTTTCTGAAGGAGCATGGAAAAATCATATCAAGGAACGAGCTTGAGAAAATTCTGTCAAAAGAGAAGACCGAAGTGATTGAGGCACCTGATATAGATGAAGCTATGGCACGAGCTGTTGACATTGGCGAAAATGATGATGAAGGAGAAGATAGTGAGGCAGAGGTTGATGAACAAGGGGAGAAAATCACACGCAATTGGAGTGTTTTGAAAAGTACTCCTCAGCTTACCAAGTCGAAGGAAAAACCCAAAAAGGAAGGTCCCATGTCTTTGGATGAGGCTGTAGACGACTCAGAGAACTTAACTGATTTCCTTATGGATTTTGAGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0003727 GO:0005488 GO:0008150 GO:0010468 GO:0019222 GO:0050789 GO:0060255 GO:0065007 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

548

Amino Acids

62.5

Weight (kDa)

4.84

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 626
AccB1I GGYRCC 1 cut(s) 1396
AccB7I CCANNNNNTGG 2 cut(s) 347, 461
AccI GTMKAC 1 cut(s) 1596
AciI CCGC 4 cut(s) 396, 554, 740, 1183
AclWI GGATC 6 cut(s) 704, 1157, 1170, 1197, 1211, 1244
AcsI RAATTY 1 cut(s) 1362
AcuI CTGAAG 2 cut(s) 408, 1340
AcyI GRCGYC 1 cut(s) 691
AfaI GTAC 3 cut(s) 52, 1226, 1528
AfiI CCNNNNNNNGG 4 cut(s) 158, 347, 461, 650
AflII CTTAAG 1 cut(s) 139
AflIII ACRYGT 1 cut(s) 1095
AgsI TTSAA 8 cut(s) 46, 94, 596, 950, 1046, 1058, 1247, 1522
AhdI GACNNNNNGTC 1 cut(s) 399
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 7 cut(s) 334, 871, 953, 1354, 1415, 1427, 1537
AluI AGCT 7 cut(s) 334, 871, 953, 1354, 1415, 1427, 1537
Alw21I GWGCWC 2 cut(s) 298, 355
Alw26I GTCTC 3 cut(s) 848, 1017, 1055
Alw44I GTGCAC 1 cut(s) 294
AlwI GGATC 6 cut(s) 704, 1157, 1170, 1197, 1211, 1244
AlwNI CAGNNNCTG 2 cut(s) 347, 857
Ama87I CYCGRG 3 cut(s) 425, 529, 1032
AoxI GGCC 2 cut(s) 383, 1276
ApaLI GTGCAC 1 cut(s) 294
ApeKI GCWGC 3 cut(s) 74, 331, 413
ApoI RAATTY 1 cut(s) 1362
ArsI GACNNNNNNTTYG 2 cut(s) 1295, 1327
Asp700I GAANNNNTTC 2 cut(s) 945, 1041
AspS9I GGNCC 7 cut(s) 161, 393, 641, 795, 980, 1277, 1571
AsuC2I CCSGG 1 cut(s) 369
AsuHPI GGTGA 3 cut(s) 292, 449, 1079
AvaI CYCGRG 3 cut(s) 425, 529, 1032
AvaII GGWCC 6 cut(s) 161, 393, 641, 795, 980, 1571
BaeGI GKGCMC 1 cut(s) 298
BamHI GGATCC 1 cut(s) 1162
BanI GGYRCC 1 cut(s) 1396
BauI CACGAG 2 cut(s) 354, 1422
BbsI GAAGAC 2 cut(s) 603, 1385
Bbv12I GWGCWC 2 cut(s) 298, 355
BbvCI CCTCAGC 1 cut(s) 1533
BbvI GCAGC 3 cut(s) 61, 343, 400
BccI CCATC 3 cut(s) 463, 795, 1129
BciT130I CCWGG 1 cut(s) 474
BcnI CCSGG 1 cut(s) 369
BcoDI GTCTC 3 cut(s) 848, 1017, 1055
BfaI CTAG 4 cut(s) 335, 399, 551, 674
BfmI CTRYAG 2 cut(s) 304, 1593
BfrI CTTAAG 1 cut(s) 139
BisI GCNGC 5 cut(s) 75, 332, 414, 555, 740
BlsI GCNGC 5 cut(s) 76, 333, 415, 556, 741
BmcAI AGTACT 1 cut(s) 1528
Bme1390I CCNGG 2 cut(s) 369, 474
Bme18I GGWCC 6 cut(s) 161, 393, 641, 795, 980, 1571
BmeRI GACNNNNNGTC 1 cut(s) 399
BmeT110I CYCGRG 3 cut(s) 425, 529, 1032
BmgT120I GGNCC 7 cut(s) 161, 393, 641, 795, 980, 1277, 1571
BmiI GGNNCC 8 cut(s) 83, 162, 394, 570, 642, 1164, 1398, 1573
BmrFI CCNGG 2 cut(s) 369, 474
BmrI ACTGGG 1 cut(s) 867
BmsI GCATC 3 cut(s) 14, 353, 509
BmuI ACTGGG 1 cut(s) 867
BpiI GAAGAC 2 cut(s) 603, 1385
BpmI CTGGAG 1 cut(s) 790
Bpu10I CCTNAGC 1 cut(s) 1533
BpuEI CTTGAG 1 cut(s) 1376
BpuMI CCSGG 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 691
BsaJI CCNNGG 1 cut(s) 152
BsaXI ACNNNNNCTCC 2 cut(s) 1206, 1236
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 347, 461, 650
Bse1I ACTGG 7 cut(s) 125, 439, 773, 862, 972, 1069, 1220
Bse3DI GCAATG 3 cut(s) 570, 852, 1179
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 1 cut(s) 152
BseGI GGATG 2 cut(s) 697, 1591
BseLI CCNNNNNNNGG 4 cut(s) 158, 347, 461, 650
BseMI GCAATG 3 cut(s) 570, 852, 1179
BseMII CTCAG 2 cut(s) 1547, 1617
BseNI ACTGG 7 cut(s) 125, 439, 773, 862, 972, 1069, 1220
BseRI GAGGAG 3 cut(s) 169, 499, 1521
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 3 cut(s) 61, 343, 400
BseYI CCCAGC 1 cut(s) 77
BshFI GGCC 2 cut(s) 385, 1278
BshNI GGYRCC 1 cut(s) 1396
BsiHKAI GWGCWC 2 cut(s) 298, 355
BsiHKCI CYCGRG 3 cut(s) 425, 529, 1032
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 5 cut(s) 174, 406, 654, 1153, 1557
BslI CCNNNNNNNGG 4 cut(s) 158, 347, 461, 650
BsmAI GTCTC 3 cut(s) 848, 1017, 1055
BsmFI GGGAC 5 cut(s) 174, 406, 654, 1153, 1557
BsmI GAATGC 2 cut(s) 363, 1185
BsnI GGCC 2 cut(s) 385, 1278
BsoBI CYCGRG 3 cut(s) 425, 529, 1032
Bsp1286I GDGCHC 2 cut(s) 298, 355
Bsp143I GATC 7 cut(s) 116, 709, 1114, 1162, 1189, 1216, 1249
BspACI CCGC 4 cut(s) 396, 554, 740, 1183
BspANI GGCC 2 cut(s) 385, 1278
BspCNI CTCAG 2 cut(s) 1546, 1616
BspHI TCATGA 2 cut(s) 514, 1288
BspLI GGNNCC 8 cut(s) 83, 162, 394, 570, 642, 1164, 1398, 1573
BspPI GGATC 6 cut(s) 704, 1157, 1170, 1197, 1211, 1244
BspQI GCTCTTC 2 cut(s) 729, 876
BspT107I GGYRCC 1 cut(s) 1396
BspTI CTTAAG 1 cut(s) 139
BsrDI GCAATG 3 cut(s) 570, 852, 1179
BsrI ACTGG 7 cut(s) 125, 439, 773, 862, 972, 1069, 1220
BssECI CCNNGG 1 cut(s) 152
BssMI GATC 7 cut(s) 116, 709, 1114, 1162, 1189, 1216, 1249
BssNI GRCGYC 1 cut(s) 691
BssSI CACGAG 2 cut(s) 354, 1422
Bst2BI CACGAG 2 cut(s) 354, 1422
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 5 cut(s) 55, 286, 824, 832, 1234
Bst6I CTCTTC 6 cut(s) 526, 729, 876, 1013, 1053, 1635
BstACI GRCGYC 1 cut(s) 691
BstAFI CTTAAG 1 cut(s) 139
BstAPI GCANNNNNTGC 1 cut(s) 350
BstC8I GCNNGC 2 cut(s) 294, 361
BstDEI CTNAG 3 cut(s) 720, 1533, 1603
BstENI CCTNNNNNAGG 1 cut(s) 648
BstF5I GGATG 2 cut(s) 697, 1591
BstKTI GATC 7 cut(s) 119, 712, 1117, 1165, 1192, 1219, 1252
BstMAI GTCTC 3 cut(s) 848, 1017, 1055
BstMBI GATC 7 cut(s) 116, 709, 1114, 1162, 1189, 1216, 1249
BstMWI GCNNNNNNNGC 5 cut(s) 350, 419, 560, 811, 868
BstNI CCWGG 1 cut(s) 474
BstNSI RCATGY 1 cut(s) 1099
BstSCI CCNGG 2 cut(s) 367, 472
BstSFI CTRYAG 2 cut(s) 304, 1593
BstSLI GKGCMC 1 cut(s) 298
BstV1I GCAGC 3 cut(s) 61, 343, 400
BstV2I GAAGAC 2 cut(s) 603, 1385
BstX2I RGATCY 3 cut(s) 1162, 1216, 1249
BstXI CCANNNNNNTGG 2 cut(s) 77, 1582
BstYI RGATCY 3 cut(s) 1162, 1216, 1249
BsuRI GGCC 2 cut(s) 385, 1278
BtsCI GGATG 2 cut(s) 697, 1591
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 3 cut(s) 564, 829, 1227
Cac8I GCNNGC 2 cut(s) 294, 361
CaiI CAGNNNCTG 2 cut(s) 347, 857
CciI TCATGA 2 cut(s) 514, 1288
Cfr13I GGNCC 7 cut(s) 161, 393, 641, 795, 980, 1277, 1571
CseI GACGC 1 cut(s) 699
Csp6I GTAC 3 cut(s) 51, 1225, 1527
CviAII CATG 7 cut(s) 71, 87, 515, 1096, 1289, 1328, 1576
CviQI GTAC 3 cut(s) 51, 1225, 1527
DdeI CTNAG 3 cut(s) 720, 1533, 1603
DpnI GATC 7 cut(s) 118, 711, 1116, 1164, 1191, 1218, 1251
DpnII GATC 7 cut(s) 116, 709, 1114, 1162, 1189, 1216, 1249
DraI TTTAAA 1 cut(s) 604
DrdI GACNNNNNNGTC 1 cut(s) 626
DriI GACNNNNNGTC 1 cut(s) 399
DseDI GACNNNNNNGTC 1 cut(s) 626
Eam1104I CTCTTC 6 cut(s) 526, 729, 876, 1013, 1053, 1635
Eam1105I GACNNNNNGTC 1 cut(s) 399
EarI CTCTTC 6 cut(s) 526, 729, 876, 1013, 1053, 1635
Eco32I GATATC 2 cut(s) 610, 1147
Eco47I GGWCC 6 cut(s) 161, 393, 641, 795, 980, 1571
Eco57I CTGAAG 2 cut(s) 408, 1340
Eco88I CYCGRG 3 cut(s) 425, 529, 1032
EcoNI CCTNNNNNAGG 1 cut(s) 648
EcoO109I RGGNCCY 4 cut(s) 161, 641, 1277, 1571
EcoRII CCWGG 1 cut(s) 472
EcoRV GATATC 2 cut(s) 610, 1147
FaeI CATG 7 cut(s) 74, 90, 518, 1099, 1292, 1331, 1579
FalI AAGNNNNNCTT 6 cut(s) 726, 758, 855, 887, 930, 962
FaqI GGGAC 5 cut(s) 174, 406, 654, 1153, 1557
FatI CATG 7 cut(s) 70, 86, 514, 1095, 1288, 1327, 1575
FauI CCCGC 1 cut(s) 1176
FblI GTMKAC 1 cut(s) 1596
Fnu4HI GCNGC 5 cut(s) 75, 332, 414, 555, 740
FokI GGATG 2 cut(s) 684, 1598
Fsp4HI GCNGC 5 cut(s) 75, 332, 414, 555, 740
FspBI CTAG 4 cut(s) 335, 399, 551, 674
GluI GCNGC 5 cut(s) 75, 332, 414, 555, 740
GsaI CCCAGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 790
HaeIII GGCC 2 cut(s) 385, 1278
HapII CCGG 1 cut(s) 369
HgaI GACGC 1 cut(s) 699
Hin1I GRCGYC 1 cut(s) 691
Hin1II CATG 7 cut(s) 74, 90, 518, 1099, 1292, 1331, 1579
HincII GTYRAC 2 cut(s) 1237, 1432
HindII GTYRAC 2 cut(s) 1237, 1432
HindIII AAGCTT 1 cut(s) 869
HinfI GANTC 7 cut(s) 200, 227, 254, 317, 440, 518, 1601
HpaI GTTAAC 1 cut(s) 1237
HpaII CCGG 1 cut(s) 369
HphI GGTGA 3 cut(s) 292, 449, 1079
Hpy166II GTNNAC 7 cut(s) 296, 548, 983, 1225, 1237, 1432, 1597
Hpy188I TCNGA 7 cut(s) 526, 619, 636, 1000, 1151, 1320, 1606
Hpy8I GTNNAC 7 cut(s) 296, 548, 983, 1225, 1237, 1432, 1597
HpyAV CCTTC 5 cut(s) 383, 1315, 1448, 1543, 1562
HpyCH4III ACNGT 5 cut(s) 55, 286, 824, 832, 1234
HpyCH4V TGCA 3 cut(s) 296, 422, 989
HpyF10VI GCNNNNNNNGC 5 cut(s) 350, 419, 560, 811, 868
HpyF3I CTNAG 3 cut(s) 720, 1533, 1603
Hsp92I GRCGYC 1 cut(s) 691
Hsp92II CATG 7 cut(s) 74, 90, 518, 1099, 1292, 1331, 1579
KspAI GTTAAC 1 cut(s) 1237
Kzo9I GATC 7 cut(s) 116, 709, 1114, 1162, 1189, 1216, 1249
LguI GCTCTTC 2 cut(s) 729, 876
LmnI GCTCC 1 cut(s) 1324
Lsp1109I GCAGC 3 cut(s) 61, 343, 400
LweI GCATC 3 cut(s) 14, 353, 509
MaeI CTAG 4 cut(s) 335, 399, 551, 674
MaeIII GTNAC 3 cut(s) 268, 280, 749
MalI GATC 7 cut(s) 118, 711, 1116, 1164, 1191, 1218, 1251
MboI GATC 7 cut(s) 116, 709, 1114, 1162, 1189, 1216, 1249
MfeI CAATTG 2 cut(s) 591, 1507
MflI RGATCY 3 cut(s) 1162, 1216, 1249
MhlI GDGCHC 2 cut(s) 298, 355
MluCI AATT 5 cut(s) 94, 591, 1089, 1362, 1507
MlyI GAGTC 4 cut(s) 311, 449, 512, 1595
MroXI GAANNNNTTC 2 cut(s) 945, 1041
MseI TTAA 5 cut(s) 140, 603, 933, 1236, 1613
MslI CAYNNNNRTG 1 cut(s) 1133
MspCI CTTAAG 1 cut(s) 139
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 2 cut(s) 369, 474
MunI CAATTG 2 cut(s) 591, 1507
Mva1269I GAATGC 2 cut(s) 363, 1185
MvaI CCWGG 1 cut(s) 474
MwoI GCNNNNNNNGC 5 cut(s) 350, 419, 560, 811, 868
NciI CCSGG 1 cut(s) 369
NdeII GATC 7 cut(s) 116, 709, 1114, 1162, 1189, 1216, 1249
NlaIII CATG 7 cut(s) 74, 90, 518, 1099, 1292, 1331, 1579
NlaIV GGNNCC 8 cut(s) 83, 162, 394, 570, 642, 1164, 1398, 1573
NmeAIII GCCGAG 1 cut(s) 177
NmuCI GTSAC 2 cut(s) 280, 749
NspI RCATGY 1 cut(s) 1099
PaeR7I CTCGAG 1 cut(s) 1032
PagI TCATGA 2 cut(s) 514, 1288
PciI ACATGT 1 cut(s) 1095
PciSI GCTCTTC 2 cut(s) 729, 876
PctI GAATGC 2 cut(s) 363, 1185
PdmI GAANNNNTTC 2 cut(s) 945, 1041
PfeI GAWTC 3 cut(s) 200, 227, 254
PflMI CCANNNNNTGG 2 cut(s) 347, 461
PfoI TCCNGGA 2 cut(s) 367, 472
PkrI GCNGC 5 cut(s) 76, 333, 415, 556, 741
PleI GAGTC 4 cut(s) 311, 448, 512, 1595
PpsI GAGTC 4 cut(s) 311, 448, 512, 1595
PpuMI RGGWCCY 3 cut(s) 161, 641, 1571
PscI ACATGT 1 cut(s) 1095
Psp5II RGGWCCY 3 cut(s) 161, 641, 1571
Psp6I CCWGG 1 cut(s) 472
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 8 cut(s) 83, 162, 394, 570, 642, 1164, 1398, 1573
PspPI GGNCC 7 cut(s) 161, 393, 641, 795, 980, 1277, 1571
PspPPI RGGWCCY 3 cut(s) 161, 641, 1571
PstNI CAGNNNCTG 2 cut(s) 347, 857
PsuI RGATCY 3 cut(s) 1162, 1216, 1249
RsaI GTAC 3 cut(s) 52, 1226, 1528
RsaNI GTAC 3 cut(s) 51, 1225, 1527
RseI CAYNNNNRTG 1 cut(s) 1133
SapI GCTCTTC 2 cut(s) 729, 876
SaqAI TTAA 5 cut(s) 140, 603, 933, 1236, 1613
SatI GCNGC 5 cut(s) 75, 332, 414, 555, 740
Sau3AI GATC 7 cut(s) 116, 709, 1114, 1162, 1189, 1216, 1249
Sau96I GGNCC 7 cut(s) 161, 393, 641, 795, 980, 1277, 1571
ScaI AGTACT 1 cut(s) 1528
SchI GAGTC 4 cut(s) 311, 449, 512, 1595
ScrFI CCNGG 2 cut(s) 369, 474
SduI GDGCHC 2 cut(s) 298, 355
SfaNI GCATC 3 cut(s) 14, 353, 509
SfcI CTRYAG 2 cut(s) 304, 1593
Sfr274I CTCGAG 1 cut(s) 1032
SinI GGWCC 6 cut(s) 161, 393, 641, 795, 980, 1571
SlaI CTCGAG 1 cut(s) 1032
SmiMI CAYNNNNRTG 1 cut(s) 1133
SmlI CTYRAG 3 cut(s) 139, 1032, 1355
SmoI CTYRAG 3 cut(s) 139, 1032, 1355
Sse9I AATT 5 cut(s) 94, 591, 1089, 1362, 1507
SsiI CCGC 4 cut(s) 396, 554, 740, 1183
SspMI CTAG 4 cut(s) 335, 399, 551, 674
StyD4I CCNGG 2 cut(s) 367, 472
TaaI ACNGT 5 cut(s) 55, 286, 824, 832, 1234
TaqI TCGA 4 cut(s) 288, 403, 1033, 1547
TaqII GACCGA 1 cut(s) 1397
TasI AATT 5 cut(s) 94, 591, 1089, 1362, 1507
TatI WGTACW 1 cut(s) 1526
TauI GCSGC 2 cut(s) 557, 742
TfiI GAWTC 3 cut(s) 200, 227, 254
Tru1I TTAA 5 cut(s) 140, 603, 933, 1236, 1613
Tru9I TTAA 5 cut(s) 140, 603, 933, 1236, 1613
TscAI CASTG 3 cut(s) 564, 829, 1227
TseFI GTSAC 2 cut(s) 280, 749
TseI GCWGC 3 cut(s) 74, 331, 413
Tsp45I GTSAC 2 cut(s) 280, 749
TspRI CASTG 3 cut(s) 564, 829, 1227
Van91I CCANNNNNTGG 2 cut(s) 347, 461
Vha464I CTTAAG 1 cut(s) 139
VneI GTGCAC 1 cut(s) 294
VpaK11BI GGWCC 6 cut(s) 161, 393, 641, 795, 980, 1571
XagI CCTNNNNNAGG 1 cut(s) 648
XapI RAATTY 1 cut(s) 1362
XceI RCATGY 1 cut(s) 1099
XhoI CTCGAG 1 cut(s) 1032
XmiI GTMKAC 1 cut(s) 1596
XmnI GAANNNNTTC 2 cut(s) 945, 1041
XspI CTAG 4 cut(s) 335, 399, 551, 674
ZrmI AGTACT 1 cut(s) 1528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.