pycom05g27870

Protein LIGHT-DEPENDENT SHORT HYPOCOTYLS 10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013800
Physical Location & Seq
Reverse (-)
28747284 .. 28748045
762 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g27870.1

Sequence Viewer

Length: 762 bp
ATGACAAGTCCTCTCTATAGACTTCATCAAGATGTGATTTTCATCTTTCTTTCATTGCACTATAACCTATTCATTCTTTCTTCCACACACCTCTCTCTCTCTCTCTCTATTTGCTTCCTTCTTTTTCCCCTTGTCATTTCTCCATTTCTTGCAAAAAATACACAGGAATTCCTCCACATCCATCACCACGATAATATCATGTCTGCACCCCATCATGATCGATGTCATCGAAGAAAAGACTCCACTCCCATCGAGGGCGCCTCCTCCTCCTCATCACGATCCACTTCTCAACAACAGCAAGCACCTCAACCCCTCAGCCGATACGAGTCCCAGAAACGCCGCGACTGGAACACTTTCGGACAGTACTTGAAGAACCAATCACCCCCAGTGTCACTCTCTCATTGCAATTGCAACCACGTCCTCGATTTCCTCCGCTACTTGGATCAGTTCGGAAAGACTAAGGTTCACTTGCACGGTTGCATCTTCTTTGGGCAGCCTGACCCGCCTGCCCCGTGCACCTGCCCTCTCAGGCAGGCATGGGGTAGTCTGGATGCGCTGATCGGACGCCTCCGTGCTGCCTACGAGGAGCATGGAGGGTCCCCGGAGACAAACCCTTTTGGGAATGGAGCTATTCGGGTTTACTTACGTGAAGTGAAGGAGTGTCAGGCTAAAGCAAGAGGGATTCCGTACAAGAAGAAGAAGAAGAGGAACGGTCAGCTTAAGGCAATTAACGAGGGTCCTCTGAAGCAGATCACGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF640

Protein Analysis

254

Amino Acids

28.78

Weight (kDa)

9.53

Isoelectric Point (pI)

60.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALOG_dom PF04852 96 - 220 6.4e-66 ALOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013800)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18610
fragaria_vesca FvH4_3g07770
malus_domestica MD05G1300800.v1.1 MD10G1279800.v1.1
prunus_persica Prupe.4G061700_v2.0.a1
pyrus_communis pycom05g27870 pycom10g23350
rosa_chinensis RchiOBHm_Chr5g0010811
rosa_laevigata RLG00000031783
rosa_multiflora Rmu_sc0000109.1_g000015
rosa_rugosa Rorug04G0452700
rosa_samantha Rh5AG085000 Rh5BG079700 Rh5CG092400 Rh5DG079900
rosa_wichuraiana Rw0G017960 Rw0G022240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 527
Acc36I ACCTGC 1 cut(s) 527
AccB1I GGYRCC 1 cut(s) 257
AccII CGCG 1 cut(s) 342
AciI CCGC 3 cut(s) 340, 433, 503
AclWI GGATC 2 cut(s) 273, 450
AcsI RAATTY 1 cut(s) 167
AcyI GRCGYC 2 cut(s) 258, 565
AfaI GTAC 2 cut(s) 365, 689
AfiI CCNNNNNNNGG 2 cut(s) 254, 439
AflII CTTAAG 1 cut(s) 719
AgsI TTSAA 1 cut(s) 370
AjiI CACGTC 2 cut(s) 418, 756
AluBI AGCT 2 cut(s) 629, 718
AluI AGCT 2 cut(s) 629, 718
Alw21I GWGCWC 1 cut(s) 518
Alw26I GTCTC 1 cut(s) 599
Alw44I GTGCAC 1 cut(s) 514
AlwI GGATC 2 cut(s) 273, 450
ApaLI GTGCAC 1 cut(s) 514
ApeKI GCWGC 2 cut(s) 493, 575
ApoI RAATTY 1 cut(s) 167
Asp700I GAANNNNTTC 1 cut(s) 353
AspLEI GCGC 2 cut(s) 260, 556
AspS9I GGNCC 2 cut(s) 597, 737
AsuC2I CCSGG 1 cut(s) 602
AsuHPI GGTGA 2 cut(s) 176, 372
AvaII GGWCC 2 cut(s) 597, 737
BaeGI GKGCMC 1 cut(s) 518
BanI GGYRCC 1 cut(s) 257
Bbv12I GWGCWC 1 cut(s) 518
BbvCI CCTCAGC 1 cut(s) 314
BbvI GCAGC 2 cut(s) 505, 562
BccI CCATC 3 cut(s) 189, 219, 257
BcnI CCSGG 1 cut(s) 602
BcoDI GTCTC 1 cut(s) 599
BfmI CTRYAG 1 cut(s) 16
BfoI RGCGCY 1 cut(s) 261
BfrI CTTAAG 1 cut(s) 719
BfuAI ACCTGC 1 cut(s) 527
BisI GCNGC 3 cut(s) 340, 494, 576
BlsI GCNGC 3 cut(s) 341, 495, 577
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 1 cut(s) 602
Bme18I GGWCC 2 cut(s) 597, 737
BmgBI CACGTC 2 cut(s) 418, 756
BmgT120I GGNCC 2 cut(s) 597, 737
BmiI GGNNCC 4 cut(s) 259, 598, 599, 738
BmrFI CCNGG 1 cut(s) 602
BmrI ACTGGG 1 cut(s) 380
BmsI GCATC 2 cut(s) 489, 541
BmuI ACTGGG 1 cut(s) 380
BplI GAGNNNNNCTC 2 cut(s) 245, 277
Bpu10I CCTNAGC 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 602
Bsa29I ATCGAT 1 cut(s) 220
BsaAI YACGTR 1 cut(s) 647
BsaBI GATNNNNATC 1 cut(s) 41
BsaHI GRCGYC 2 cut(s) 258, 565
BsaJI CCNNGG 1 cut(s) 600
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 439
Bse1I ACTGG 2 cut(s) 350, 386
Bse3DI GCAATG 2 cut(s) 53, 400
Bse8I GATNNNNATC 1 cut(s) 41
BseCI ATCGAT 1 cut(s) 220
BseDI CCNNGG 1 cut(s) 600
BseGI GGATG 2 cut(s) 177, 556
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 2 cut(s) 254, 439
BseMI GCAATG 2 cut(s) 53, 400
BseMII CTCAG 2 cut(s) 328, 541
BseNI ACTGG 2 cut(s) 350, 386
BseRI GAGGAG 4 cut(s) 253, 256, 259, 599
BseSI GKGCMC 1 cut(s) 518
BseXI GCAGC 2 cut(s) 505, 562
BsgI GTGCAG 1 cut(s) 189
Bsh1236I CGCG 1 cut(s) 342
BshNI GGYRCC 1 cut(s) 257
BshVI ATCGAT 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 518
BsiSI CCGG 1 cut(s) 602
BslFI GGGAC 2 cut(s) 313, 583
BslI CCNNNNNNNGG 2 cut(s) 254, 439
BsmAI GTCTC 1 cut(s) 599
BsmFI GGGAC 2 cut(s) 313, 583
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 5 cut(s) 217, 278, 442, 558, 750
BspACI CCGC 3 cut(s) 340, 433, 503
BspCNI CTCAG 2 cut(s) 327, 540
BspDI ATCGAT 1 cut(s) 220
BspFNI CGCG 1 cut(s) 342
BspHI TCATGA 1 cut(s) 214
BspLI GGNNCC 4 cut(s) 259, 598, 599, 738
BspMI ACCTGC 1 cut(s) 527
BspPI GGATC 2 cut(s) 273, 450
BspT107I GGYRCC 1 cut(s) 257
BspTI CTTAAG 1 cut(s) 719
BsrDI GCAATG 2 cut(s) 53, 400
BsrI ACTGG 2 cut(s) 350, 386
BssECI CCNNGG 1 cut(s) 600
BssMI GATC 5 cut(s) 217, 278, 442, 558, 750
BssNI GRCGYC 2 cut(s) 258, 565
Bst4CI ACNGT 3 cut(s) 363, 476, 713
Bst6I CTCTTC 1 cut(s) 698
BstACI GRCGYC 2 cut(s) 258, 565
BstAFI CTTAAG 1 cut(s) 719
BstBAI YACGTR 1 cut(s) 647
BstC8I GCNNGC 3 cut(s) 300, 507, 534
BstDEI CTNAG 3 cut(s) 314, 459, 527
BstF5I GGATG 2 cut(s) 177, 556
BstFNI CGCG 1 cut(s) 342
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 2 cut(s) 260, 556
BstKTI GATC 5 cut(s) 220, 281, 445, 561, 753
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 5 cut(s) 217, 278, 442, 558, 750
BstMWI GCNNNNNNNGC 1 cut(s) 502
BstSCI CCNGG 1 cut(s) 600
BstSFI CTRYAG 1 cut(s) 16
BstSLI GKGCMC 1 cut(s) 518
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 2 cut(s) 505, 562
Bsu15I ATCGAT 1 cut(s) 220
BsuTUI ATCGAT 1 cut(s) 220
BtrI CACGTC 2 cut(s) 418, 756
BtsCI GGATG 2 cut(s) 177, 556
BtsIMutI CAGTG 1 cut(s) 393
BveI ACCTGC 1 cut(s) 527
Cac8I GCNNGC 3 cut(s) 300, 507, 534
CciI TCATGA 1 cut(s) 214
CfoI GCGC 2 cut(s) 260, 556
Cfr13I GGNCC 2 cut(s) 597, 737
ClaI ATCGAT 1 cut(s) 220
CseI GACGC 1 cut(s) 573
Csp6I GTAC 2 cut(s) 364, 688
CviAII CATG 4 cut(s) 199, 215, 537, 590
CviJI RGCY 5 cut(s) 318, 496, 629, 668, 718
CviKI_1 RGCY 5 cut(s) 318, 496, 629, 668, 718
CviQI GTAC 2 cut(s) 364, 688
DdeI CTNAG 3 cut(s) 314, 459, 527
DinI GGCGCC 1 cut(s) 259
DpnI GATC 5 cut(s) 219, 280, 444, 560, 752
DpnII GATC 5 cut(s) 217, 278, 442, 558, 750
Eam1104I CTCTTC 1 cut(s) 698
EarI CTCTTC 1 cut(s) 698
Eco47I GGWCC 2 cut(s) 597, 737
EcoO109I RGGNCCY 2 cut(s) 597, 737
EcoRI GAATTC 1 cut(s) 167
EgeI GGCGCC 1 cut(s) 259
EheI GGCGCC 1 cut(s) 259
FaeI CATG 4 cut(s) 202, 218, 540, 593
FaiI YATR 6 cut(s) 18, 63, 200, 216, 538, 591
FalI AAGNNNNNCTT 2 cut(s) 452, 484
FaqI GGGAC 2 cut(s) 313, 583
FatI CATG 4 cut(s) 198, 214, 536, 589
FauI CCCGC 1 cut(s) 510
Fnu4HI GCNGC 3 cut(s) 340, 494, 576
FokI GGATG 2 cut(s) 164, 563
Fsp4HI GCNGC 3 cut(s) 340, 494, 576
GlaI GCGC 2 cut(s) 259, 555
GluI GCNGC 3 cut(s) 340, 494, 576
HaeII RGCGCY 1 cut(s) 261
HapII CCGG 1 cut(s) 602
HgaI GACGC 1 cut(s) 573
HhaI GCGC 2 cut(s) 260, 556
Hin1I GRCGYC 2 cut(s) 258, 565
Hin1II CATG 4 cut(s) 202, 218, 540, 593
Hin6I GCGC 2 cut(s) 258, 554
HinP1I GCGC 2 cut(s) 258, 554
HinfI GANTC 3 cut(s) 239, 326, 682
HpaII CCGG 1 cut(s) 602
HphI GGTGA 2 cut(s) 176, 372
Hpy166II GTNNAC 3 cut(s) 466, 516, 640
Hpy188I TCNGA 4 cut(s) 359, 452, 563, 744
Hpy188III TCNNGA 5 cut(s) 29, 215, 276, 548, 759
Hpy8I GTNNAC 3 cut(s) 466, 516, 640
HpyAV CCTTC 2 cut(s) 128, 649
HpyCH4III ACNGT 3 cut(s) 363, 476, 713
HpyCH4IV ACGT 3 cut(s) 417, 646, 755
HpyCH4V TGCA 8 cut(s) 58, 152, 206, 405, 411, 472, 480, 516
HpyF10VI GCNNNNNNNGC 1 cut(s) 502
HpyF3I CTNAG 3 cut(s) 314, 459, 527
HpySE526I ACGT 3 cut(s) 417, 646, 755
Hsp92I GRCGYC 2 cut(s) 258, 565
Hsp92II CATG 4 cut(s) 202, 218, 540, 593
HspAI GCGC 2 cut(s) 258, 554
KasI GGCGCC 1 cut(s) 257
KflI GGGWCCC 1 cut(s) 597
Kzo9I GATC 5 cut(s) 217, 278, 442, 558, 750
LmnI GCTCC 2 cut(s) 586, 626
Lsp1109I GCAGC 2 cut(s) 505, 562
LweI GCATC 2 cut(s) 489, 541
MaeII ACGT 3 cut(s) 417, 646, 755
MaeIII GTNAC 1 cut(s) 390
MalI GATC 5 cut(s) 219, 280, 444, 560, 752
MboI GATC 5 cut(s) 217, 278, 442, 558, 750
MboII GAAGA 8 cut(s) 72, 243, 382, 475, 706, 709, 712, 715
MfeI CAATTG 1 cut(s) 406
MhlI GDGCHC 1 cut(s) 518
MluCI AATT 3 cut(s) 167, 406, 726
Mly113I GGCGCC 1 cut(s) 258
MlyI GAGTC 2 cut(s) 233, 335
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 2 cut(s) 720, 729
MslI CAYNNNNRTG 1 cut(s) 30
MspCI CTTAAG 1 cut(s) 719
MspI CCGG 1 cut(s) 602
MspR9I CCNGG 1 cut(s) 602
MunI CAATTG 1 cut(s) 406
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 1 cut(s) 502
NarI GGCGCC 1 cut(s) 258
NciI CCSGG 1 cut(s) 602
NdeII GATC 5 cut(s) 217, 278, 442, 558, 750
NlaIII CATG 4 cut(s) 202, 218, 540, 593
NlaIV GGNNCC 4 cut(s) 259, 598, 599, 738
NmuCI GTSAC 1 cut(s) 390
PagI TCATGA 1 cut(s) 214
PaqCI CACCTGC 1 cut(s) 527
PcsI WCGNNNNNNNCGW 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 1 cut(s) 682
PkrI GCNGC 3 cut(s) 341, 495, 577
PleI GAGTC 2 cut(s) 233, 334
PluTI GGCGCC 1 cut(s) 261
PpsI GAGTC 2 cut(s) 233, 334
Ppu21I YACGTR 1 cut(s) 647
PpuMI RGGWCCY 2 cut(s) 597, 737
Psp5II RGGWCCY 2 cut(s) 597, 737
PspN4I GGNNCC 4 cut(s) 259, 598, 599, 738
PspPI GGNCC 2 cut(s) 597, 737
PspPPI RGGWCCY 2 cut(s) 597, 737
RsaI GTAC 2 cut(s) 365, 689
RsaNI GTAC 2 cut(s) 364, 688
RseI CAYNNNNRTG 1 cut(s) 30
SaqAI TTAA 2 cut(s) 720, 729
SatI GCNGC 3 cut(s) 340, 494, 576
Sau3AI GATC 5 cut(s) 217, 278, 442, 558, 750
Sau96I GGNCC 2 cut(s) 597, 737
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 2 cut(s) 233, 335
ScrFI CCNGG 1 cut(s) 602
SduI GDGCHC 1 cut(s) 518
SfaNI GCATC 2 cut(s) 489, 541
SfcI CTRYAG 1 cut(s) 16
SfoI GGCGCC 1 cut(s) 259
SinI GGWCC 2 cut(s) 597, 737
SmiMI CAYNNNNRTG 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
Sse9I AATT 3 cut(s) 167, 406, 726
SsiI CCGC 3 cut(s) 340, 433, 503
SspDI GGCGCC 1 cut(s) 257
StyD4I CCNGG 1 cut(s) 600
TaaI ACNGT 3 cut(s) 363, 476, 713
TaiI ACGT 3 cut(s) 420, 649, 758
TaqI TCGA 4 cut(s) 220, 229, 252, 423
TasI AATT 3 cut(s) 167, 406, 726
TatI WGTACW 1 cut(s) 363
TauI GCSGC 1 cut(s) 342
TfiI GAWTC 1 cut(s) 682
Tru1I TTAA 2 cut(s) 720, 729
Tru9I TTAA 2 cut(s) 720, 729
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 390
TseI GCWGC 2 cut(s) 493, 575
Tsp45I GTSAC 1 cut(s) 390
TspDTI ATGAA 4 cut(s) 14, 31, 42, 61
TspGWI ACGGA 2 cut(s) 560, 675
TspRI CASTG 1 cut(s) 393
Vha464I CTTAAG 1 cut(s) 719
VneI GTGCAC 1 cut(s) 514
VpaK11BI GGWCC 2 cut(s) 597, 737
XapI RAATTY 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 353
ZrmI AGTACT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.