pycom05g31040

dnaJ homolog subfamily C member 25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013208
Physical Location & Seq
Forward (+)
30938721 .. 30941303
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 924 bp
ATGGGTTTGTCATTAAACGCGCTCCTGCTCTCGGAATCCATTTTCAGAGAAATCCGTAGCTCCGTCGGAACCATGGCGCCACCGGCAGCGATTCGGTGGTTCGCGGTCGTTTCATTTCTAATGCTGTTCCAGCTCATCACTCCATCGATGGCCATCTACTGCGACGAGGACGACTGCTACGATCTCCTCGGGGTTACGCAGAGCGCCAATTCGTCGGAAATCAAGAAAGCTTACTACAAGCTCTCATTGAAACATCACCCGGATAAAAACCCGGATCCGGAATCGAAAAAGCTGTTTGTGAAAATTGCCAACGCTTATGAGATTCTGAAAGACGAAGCGTCGCGCGAGCAGTATGATTATGCCATTGCACATCCAGAGGAGGATCCTCGGGCTGTCCTGGTTGGCCTTCTTTTGATTCTTTCTGCATTTCAGTATCTAAATCAGTGGACAAGGTATAATCAGGCTGTAGACATGGTCAAGAAGACTCCTGCGTACAAAAATAGACTACGGGCTTTGGAACTTGAGCGTACTGGAGGGACTACAAATAAGAAGAAGAGCAACAGGCAGATGAACAAGAAAGTGGAAGAAGATCTCGGCAGCGAACTGGAGCTGCAGATTAACGGTGCGGAACAACCCTCCATGTGGGATCTTCTTGGTGTCCGTTTCATACTTCTGCCCTACACTATTGGCAAGCTATTACTATGGTGTGGCTGTTGGTTCTGGAGATACAATGTGAGAAAAGCACCATATACATGGGATGATGCCTCCTACCTGACACGGAGATCCCTTGGTGCGCCTATTGACGGATGGAGAAATCTAGATGAATCAAGAAAGGAAGATCTTATTCATAAACGTTTATGGGAAAAAGCAAACTTGGAGAGCTACCTTGCGGATATGCGCAAAGAATCCAAGCGGAGAAGATAG

Protein Analysis

308

Amino Acids

35.66

Weight (kDa)

8.94

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 58 - 119 6.6e-24 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013208)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61770
fragaria_vesca FvH4_3g02660
malus_domestica MD05G1341100.v1.1 MD10G1314600.v1.1
prunus_persica Prupe.4G026200_v2.0.a1 Prupe.4G026200_v2.0.a1
pyrus_communis pycom05g31040 pycom10g26670
rosa_chinensis RchiOBHm_Chr5g0003981
rosa_laevigata RLG00000031194
rosa_multiflora Rmu_sc0000547.1_g000013
rosa_roxburghii Rroxscaffold_1G00071980
rosa_rugosa Rorug04G0408700
rosa_samantha Rh5AG036400 Rh5BG035500 Rh5CG039100 Rh5DG035000
rosa_wichuraiana Rw5G003310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 899
AccB1I GGYRCC 1 cut(s) 76
AccI GTMKAC 1 cut(s) 468
AccII CGCG 4 cut(s) 20, 104, 343, 345
AccIII TCCGGA 1 cut(s) 277
AciI CCGC 4 cut(s) 104, 626, 890, 913
AclI AACGTT 1 cut(s) 853
AclWI GGATC 6 cut(s) 269, 282, 377, 390, 654, 777
AcoI YGGCCR 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 2 cut(s) 494, 529
AfiI CCNNNNNNNGG 4 cut(s) 31, 277, 642, 803
AgsI TTSAA 1 cut(s) 250
AhdI GACNNNNNGTC 1 cut(s) 337
AjnI CCWGG 1 cut(s) 396
AluBI AGCT 8 cut(s) 60, 133, 230, 241, 292, 610, 694, 882
AluI AGCT 8 cut(s) 60, 133, 230, 241, 292, 610, 694, 882
AlwI GGATC 6 cut(s) 269, 282, 377, 390, 654, 777
Ama87I CYCGRG 2 cut(s) 188, 387
Aor13HI TCCGGA 1 cut(s) 277
AoxI GGCC 2 cut(s) 150, 403
ApeKI GCWGC 3 cut(s) 86, 597, 610
AspLEI GCGC 6 cut(s) 22, 79, 206, 345, 796, 900
AsuC2I CCSGG 2 cut(s) 260, 272
AsuHPI GGTGA 1 cut(s) 248
AvaI CYCGRG 2 cut(s) 188, 387
BalI TGGCCA 1 cut(s) 152
BamHI GGATCC 2 cut(s) 274, 382
BanI GGYRCC 1 cut(s) 76
BbsI GAAGAC 1 cut(s) 488
BbvI GCAGC 3 cut(s) 98, 597, 609
BccI CCATC 4 cut(s) 142, 151, 161, 801
BciT130I CCWGG 1 cut(s) 398
BcnI CCSGG 2 cut(s) 260, 272
BfaI CTAG 1 cut(s) 818
BfmI CTRYAG 2 cut(s) 465, 611
BfoI RGCGCY 2 cut(s) 80, 207
BglII AGATCT 2 cut(s) 589, 838
BisI GCNGC 3 cut(s) 87, 598, 611
BlsI GCNGC 3 cut(s) 88, 599, 612
Bme1390I CCNGG 3 cut(s) 260, 272, 398
BmeRI GACNNNNNGTC 1 cut(s) 337
BmeT110I CYCGRG 2 cut(s) 188, 387
BmiI GGNNCC 4 cut(s) 70, 78, 276, 384
BmrFI CCNGG 3 cut(s) 260, 272, 398
BmsI GCATC 1 cut(s) 751
BpiI GAAGAC 1 cut(s) 488
BpmI CTGGAG 3 cut(s) 552, 626, 742
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 2 cut(s) 260, 272
Bsa29I ATCGAT 1 cut(s) 146
BsaBI GATNNNNATC 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 4 cut(s) 72, 187, 386, 787
BsaWI WCCGGW 1 cut(s) 277
Bsc4I CCNNNNNNNGG 4 cut(s) 31, 277, 642, 803
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 2 cut(s) 535, 609
Bse3DI GCAATG 1 cut(s) 363
Bse8I GATNNNNATC 1 cut(s) 152
BseAI TCCGGA 1 cut(s) 277
BseBI CCWGG 1 cut(s) 398
BseCI ATCGAT 1 cut(s) 146
BseDI CCNNGG 4 cut(s) 72, 187, 386, 787
BseGI GGATG 3 cut(s) 370, 763, 812
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 4 cut(s) 31, 277, 642, 803
BseMI GCAATG 1 cut(s) 363
BseNI ACTGG 2 cut(s) 535, 609
BseRI GAGGAG 2 cut(s) 176, 392
BseXI GCAGC 3 cut(s) 98, 597, 609
Bsh1236I CGCG 4 cut(s) 20, 104, 343, 345
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 2 cut(s) 152, 405
BshNI GGYRCC 1 cut(s) 76
BshVI ATCGAT 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 108
BsiHKCI CYCGRG 2 cut(s) 188, 387
BsiSI CCGG 4 cut(s) 83, 260, 272, 278
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 4 cut(s) 31, 277, 642, 803
BsmFI GGGAC 1 cut(s) 550
BsnI GGCC 2 cut(s) 152, 405
BsoBI CYCGRG 2 cut(s) 188, 387
Bsp13I TCCGGA 1 cut(s) 277
Bsp143I GATC 7 cut(s) 181, 274, 382, 589, 646, 782, 838
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 4 cut(s) 104, 626, 890, 913
BspANI GGCC 2 cut(s) 152, 405
BspDI ATCGAT 1 cut(s) 146
BspEI TCCGGA 1 cut(s) 277
BspFNI CGCG 4 cut(s) 20, 104, 343, 345
BspLI GGNNCC 4 cut(s) 70, 78, 276, 384
BspMAI CTGCAG 1 cut(s) 615
BspPI GGATC 6 cut(s) 269, 282, 377, 390, 654, 777
BspQI GCTCTTC 1 cut(s) 548
BspT107I GGYRCC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 363
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 2 cut(s) 535, 609
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 4 cut(s) 72, 187, 386, 787
BssMI GATC 7 cut(s) 181, 274, 382, 589, 646, 782, 838
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 2 cut(s) 72, 787
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 1 cut(s) 623
Bst6I CTCTTC 1 cut(s) 548
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 347, 692
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 3 cut(s) 370, 763, 812
BstFNI CGCG 4 cut(s) 20, 104, 343, 345
BstH2I RGCGCY 2 cut(s) 80, 207
BstHHI GCGC 6 cut(s) 22, 79, 206, 345, 796, 900
BstKTI GATC 7 cut(s) 184, 277, 385, 592, 649, 785, 841
BstMBI GATC 7 cut(s) 181, 274, 382, 589, 646, 782, 838
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 2 cut(s) 83, 130
BstNI CCWGG 1 cut(s) 398
BstSCI CCNGG 3 cut(s) 258, 270, 396
BstSFI CTRYAG 2 cut(s) 465, 611
BstUI CGCG 4 cut(s) 20, 104, 343, 345
BstV1I GCAGC 3 cut(s) 98, 597, 609
BstV2I GAAGAC 1 cut(s) 488
BstX2I RGATCY 6 cut(s) 274, 382, 589, 646, 782, 838
BstXI CCANNNNNNTGG 1 cut(s) 753
BstYI RGATCY 6 cut(s) 274, 382, 589, 646, 782, 838
Bsu15I ATCGAT 1 cut(s) 146
BsuRI GGCC 2 cut(s) 152, 405
BsuTUI ATCGAT 1 cut(s) 146
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 3 cut(s) 370, 763, 812
BtsIMutI CAGTG 1 cut(s) 449
Cac8I GCNNGC 2 cut(s) 347, 692
CfoI GCGC 6 cut(s) 22, 79, 206, 345, 796, 900
Cfr10I RCCGGY 1 cut(s) 82
ClaI ATCGAT 1 cut(s) 146
CseI GACGC 1 cut(s) 327
Csp6I GTAC 2 cut(s) 493, 528
CviAII CATG 4 cut(s) 73, 472, 640, 753
CviQI GTAC 2 cut(s) 493, 528
DinI GGCGCC 1 cut(s) 78
DpnI GATC 7 cut(s) 183, 276, 384, 591, 648, 784, 840
DpnII GATC 7 cut(s) 181, 274, 382, 589, 646, 782, 838
DriI GACNNNNNGTC 1 cut(s) 337
EaeI YGGCCR 1 cut(s) 150
Eam1104I CTCTTC 1 cut(s) 548
Eam1105I GACNNNNNGTC 1 cut(s) 337
EarI CTCTTC 1 cut(s) 548
Eco130I CCWWGG 2 cut(s) 72, 787
Eco88I CYCGRG 2 cut(s) 188, 387
EcoRII CCWGG 1 cut(s) 396
EcoT14I CCWWGG 2 cut(s) 72, 787
EgeI GGCGCC 1 cut(s) 78
EheI GGCGCC 1 cut(s) 78
ErhI CCWWGG 2 cut(s) 72, 787
FaeI CATG 4 cut(s) 76, 475, 643, 756
FaqI GGGAC 1 cut(s) 550
FatI CATG 4 cut(s) 72, 471, 639, 752
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 3 cut(s) 87, 598, 611
FokI GGATG 3 cut(s) 357, 770, 819
Fsp4HI GCNGC 3 cut(s) 87, 598, 611
FspBI CTAG 1 cut(s) 818
FspI TGCGCA 1 cut(s) 899
GlaI GCGC 6 cut(s) 21, 78, 205, 344, 795, 899
GluI GCNGC 3 cut(s) 87, 598, 611
GsuI CTGGAG 3 cut(s) 552, 626, 742
HaeII RGCGCY 2 cut(s) 80, 207
HaeIII GGCC 2 cut(s) 152, 405
HapII CCGG 4 cut(s) 83, 260, 272, 278
HgaI GACGC 1 cut(s) 327
HhaI GCGC 6 cut(s) 22, 79, 206, 345, 796, 900
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 4 cut(s) 76, 475, 643, 756
Hin6I GCGC 6 cut(s) 20, 77, 204, 343, 794, 898
HinP1I GCGC 6 cut(s) 20, 77, 204, 343, 794, 898
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 8 cut(s) 35, 91, 281, 322, 415, 484, 824, 905
HpaII CCGG 4 cut(s) 83, 260, 272, 278
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 2 cut(s) 447, 469
Hpy188I TCNGA 5 cut(s) 34, 47, 68, 217, 327
Hpy188III TCNNGA 7 cut(s) 223, 278, 374, 478, 721, 818, 828
Hpy8I GTNNAC 2 cut(s) 447, 469
Hpy99I CGWCG 4 cut(s) 68, 167, 217, 343
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 1 cut(s) 623
HpyCH4IV ACGT 1 cut(s) 853
HpyCH4V TGCA 3 cut(s) 368, 425, 613
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 130
HpySE526I ACGT 1 cut(s) 853
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 4 cut(s) 76, 475, 643, 756
HspAI GCGC 6 cut(s) 20, 77, 204, 343, 794, 898
KasI GGCGCC 1 cut(s) 76
Kpn2I TCCGGA 1 cut(s) 277
Kzo9I GATC 7 cut(s) 181, 274, 382, 589, 646, 782, 838
LguI GCTCTTC 1 cut(s) 548
LmnI GCTCC 3 cut(s) 27, 65, 607
Lsp1109I GCAGC 3 cut(s) 98, 597, 609
LweI GCATC 1 cut(s) 751
MaeI CTAG 1 cut(s) 818
MaeII ACGT 1 cut(s) 853
MaeIII GTNAC 1 cut(s) 193
MalI GATC 7 cut(s) 183, 276, 384, 591, 648, 784, 840
MboI GATC 7 cut(s) 181, 274, 382, 589, 646, 782, 838
MboII GAAGA 7 cut(s) 493, 562, 565, 596, 599, 641, 848
MflI RGATCY 6 cut(s) 274, 382, 589, 646, 782, 838
MlsI TGGCCA 1 cut(s) 152
MluCI AATT 2 cut(s) 208, 303
MluNI TGGCCA 1 cut(s) 152
Mly113I GGCGCC 1 cut(s) 77
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 2 cut(s) 46, 195
MnlI CCTC 8 cut(s) 160, 197, 370, 373, 396, 527, 646, 775
Mox20I TGGCCA 1 cut(s) 152
MroI TCCGGA 1 cut(s) 277
MscI TGGCCA 1 cut(s) 152
MseI TTAA 2 cut(s) 14, 618
MslI CAYNNNNRTG 1 cut(s) 751
Msp20I TGGCCA 1 cut(s) 152
MspI CCGG 4 cut(s) 83, 260, 272, 278
MspR9I CCNGG 3 cut(s) 260, 272, 398
MvaI CCWGG 1 cut(s) 398
MvnI CGCG 4 cut(s) 20, 104, 343, 345
MwoI GCNNNNNNNGC 2 cut(s) 83, 130
NarI GGCGCC 1 cut(s) 77
NciI CCSGG 2 cut(s) 260, 272
NcoI CCATGG 1 cut(s) 72
NdeII GATC 7 cut(s) 181, 274, 382, 589, 646, 782, 838
NlaIII CATG 4 cut(s) 76, 475, 643, 756
NlaIV GGNNCC 4 cut(s) 70, 78, 276, 384
NmeAIII GCCGAG 1 cut(s) 573
NsbI TGCGCA 1 cut(s) 899
PciSI GCTCTTC 1 cut(s) 548
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PfeI GAWTC 7 cut(s) 35, 91, 281, 322, 415, 824, 905
PflFI GACNNNGTC 1 cut(s) 473
PkrI GCNGC 3 cut(s) 88, 599, 612
PleI GAGTC 1 cut(s) 478
PluTI GGCGCC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 478
Psp1406I AACGTT 1 cut(s) 853
Psp6I CCWGG 1 cut(s) 396
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 4 cut(s) 70, 78, 276, 384
PstI CTGCAG 1 cut(s) 615
PsuI RGATCY 6 cut(s) 274, 382, 589, 646, 782, 838
PsyI GACNNNGTC 1 cut(s) 473
RsaI GTAC 2 cut(s) 494, 529
RsaNI GTAC 2 cut(s) 493, 528
RseI CAYNNNNRTG 1 cut(s) 751
SapI GCTCTTC 1 cut(s) 548
SaqAI TTAA 2 cut(s) 14, 618
SatI GCNGC 3 cut(s) 87, 598, 611
Sau3AI GATC 7 cut(s) 181, 274, 382, 589, 646, 782, 838
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 3 cut(s) 260, 272, 398
SfaNI GCATC 1 cut(s) 751
SfcI CTRYAG 2 cut(s) 465, 611
SfoI GGCGCC 1 cut(s) 78
SmiMI CAYNNNNRTG 1 cut(s) 751
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 2 cut(s) 208, 303
SsiI CCGC 4 cut(s) 104, 626, 890, 913
SspDI GGCGCC 1 cut(s) 76
SspMI CTAG 1 cut(s) 818
StyD4I CCNGG 3 cut(s) 258, 270, 396
StyI CCWWGG 2 cut(s) 72, 787
TaaI ACNGT 1 cut(s) 623
TaiI ACGT 1 cut(s) 856
TaqI TCGA 2 cut(s) 146, 284
TasI AATT 2 cut(s) 208, 303
TfiI GAWTC 7 cut(s) 35, 91, 281, 322, 415, 824, 905
Tru1I TTAA 2 cut(s) 14, 618
Tru9I TTAA 2 cut(s) 14, 618
TscAI CASTG 1 cut(s) 449
TseI GCWGC 3 cut(s) 86, 597, 610
TspDTI ATGAA 5 cut(s) 102, 584, 655, 836, 837
TspGWI ACGGA 5 cut(s) 44, 52, 650, 793, 819
TspRI CASTG 1 cut(s) 449
Tth111I GACNNNGTC 1 cut(s) 473
XbaI TCTAGA 1 cut(s) 817
XmiI GTMKAC 1 cut(s) 468
XspI CTAG 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.