pycom06g03850

Decapping nuclease DXO homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008690
Physical Location & Seq
Forward (+)
4789424 .. 4793382
3959 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1104 bp
ATGGATTTTTCAGAACACCACAAAAATATCTTCGGGCCAGACTACGACGACGACGACGACGGTAATAAACAAGATGGGGCCGGTTCATCCTCACCGTCCTCCTCCTCATCGTCGTCTTCGTCCTCATCTTCGTCCTCATCGGCTTCTTCTTCATCCAACGAAGGAGGCGAGTCCAGCAGTGGGAATAGCCCGAGCGCAAGCAGTGGCGGCGGCGCTGGTGGTGGGGAAGAAGAGGTAGAGAATGGCGAAGAGGCTGATTATTCAAATGCCGCAACCGATGACAATGAGTATAGCAATATGGGTTATTTTGGGGAGGACGACAAGGATCTGTTTGGCTCTGACAACGAAGATTACTGTAAAACCCCTGCTACTAGTCCTTTCCCTATTCCTGTATTGCCTGTCATACGCAATACGAATAATCAGGGTAGAGGGAATTCAGGGCGTGGTCGTTGGCCATCTGGACGTCCAAATGATAGAGGTGCAGGCATCCTTGGTCGAGGTGGACCTTTTCAGCAGAGGCAAAATTTTGGATATGGTGGCTCAAATGGTTATCGCGATGAACGTTTTGTCTCGGAACTAAGACTACTTTCAAAGAGCGAAGAAACACTGTCAAGAAAGGCCGTTGCATTTCAGGAACCCTGTGAGCTTGCTTGCTATAGTCGGGTAGAAGGTGGAGATGTCAGCTTTGACGAGACCAGTTTGAGGCTTTTCAAGCGTCTTATTACTGAAGAAATTGGAAGAGATCTGAATGAAGGTTATGATACTTTTATTGCGAAGAAAGATTTGGGCTCTCAAGGTTTTGGTGACCTTCTCGACTGCATAAGAGACAAGAATATTCCACTTCAAAACATTCATTTTGTGACTTATCGCAACAATCTTAATAAGATATTGGCCACTTTTTATAATAAAAGGGATCCTTGGGAGATGGGAGTGCATAAAAGGAATGGGGTTGTCTATCTTGATGTGCATAAACTACCTGAAAGGCCACAGACTGAGTTGGATCGAAGAAGGTGTTACTGGGGGTATTGTTTTGAGAGTCTTGCCACTGAAGATCCAGGACGAGGTGATGGAGGGATACATCATGTTGATGCCAATGTTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RAI1

Protein Analysis

368

Amino Acids

40.13

Weight (kDa)

4.75

Isoelectric Point (pI)

61.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAI1 PF08652 211 - 352 1.2e-29 RAI1 like PD-(D/E)XK nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 903
AatII GACGTC 1 cut(s) 466
AccII CGCG 1 cut(s) 555
AciI CCGC 3 cut(s) 207, 210, 270
AclI AACGTT 1 cut(s) 562
AclWI GGATC 5 cut(s) 333, 908, 921, 1008, 1046
AcoI YGGCCR 2 cut(s) 452, 891
AcsI RAATTY 2 cut(s) 433, 523
AcuI CTGAAG 2 cut(s) 747, 1068
AcyI GRCGYC 1 cut(s) 463
AfiI CCNNNNNNNGG 3 cut(s) 180, 702, 1061
AgsI TTSAA 5 cut(s) 264, 591, 712, 845, 1100
AhlI ACTAGT 1 cut(s) 371
AjnI CCWGG 1 cut(s) 1054
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
AluBI AGCT 2 cut(s) 646, 684
AluI AGCT 2 cut(s) 646, 684
Alw26I GTCTC 3 cut(s) 574, 686, 819
AlwI GGATC 5 cut(s) 333, 908, 921, 1008, 1046
Ama87I CYCGRG 1 cut(s) 190
AoxI GGCC 6 cut(s) 35, 78, 452, 618, 891, 983
ApoI RAATTY 2 cut(s) 433, 523
AspLEI GCGC 2 cut(s) 197, 215
AspS9I GGNCC 3 cut(s) 35, 78, 503
AsuHPI GGTGA 3 cut(s) 84, 815, 1076
AvaI CYCGRG 1 cut(s) 190
AvaII GGWCC 1 cut(s) 503
BalI TGGCCA 2 cut(s) 454, 893
BamHI GGATCC 1 cut(s) 913
BanII GRGCYC 1 cut(s) 791
BbsI GAAGAC 1 cut(s) 108
BccI CCATC 4 cut(s) 68, 463, 919, 1061
BceAI ACGGC 1 cut(s) 605
BciT130I CCWGG 1 cut(s) 1056
BciVI GTATCC 1 cut(s) 1068
BcoDI GTCTC 3 cut(s) 574, 686, 819
BcuI ACTAGT 1 cut(s) 371
BfaI CTAG 1 cut(s) 372
BfmI CTRYAG 1 cut(s) 655
BfoI RGCGCY 1 cut(s) 216
BfuI GTATCC 1 cut(s) 1068
BglII AGATCT 1 cut(s) 742
BisI GCNGC 3 cut(s) 208, 211, 270
BlsI GCNGC 3 cut(s) 209, 212, 271
Bme1390I CCNGG 1 cut(s) 1056
Bme18I GGWCC 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 190
BmgT120I GGNCC 3 cut(s) 35, 78, 503
BmiI GGNNCC 3 cut(s) 79, 636, 915
BmrFI CCNGG 1 cut(s) 1056
BmrI ACTGGG 1 cut(s) 1027
BmsI GCATC 2 cut(s) 495, 1078
BmuI ACTGGG 1 cut(s) 1027
BpiI GAAGAC 1 cut(s) 108
BpuEI CTTGAG 1 cut(s) 777
BsaHI GRCGYC 1 cut(s) 463
BsaI GGTCTC 1 cut(s) 686
BsaJI CCNNGG 2 cut(s) 490, 917
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 702, 1061
Bse118I RCCGGY 1 cut(s) 80
Bse1I ACTGG 2 cut(s) 696, 1022
BseBI CCWGG 1 cut(s) 1056
BseDI CCNNGG 2 cut(s) 490, 917
BseGI GGATG 3 cut(s) 86, 152, 486
BseLI CCNNNNNNNGG 3 cut(s) 180, 702, 1061
BseMII CTCAG 1 cut(s) 984
BseNI ACTGG 2 cut(s) 696, 1022
BseRI GAGGAG 2 cut(s) 91, 94
BsgI GTGCAG 1 cut(s) 501
Bsh1236I CGCG 1 cut(s) 555
BshFI GGCC 6 cut(s) 37, 80, 454, 620, 893, 985
BsiHKCI CYCGRG 1 cut(s) 190
BsiSI CCGG 1 cut(s) 81
BslI CCNNNNNNNGG 3 cut(s) 180, 702, 1061
BsmAI GTCTC 3 cut(s) 574, 686, 819
BsnI GGCC 6 cut(s) 37, 80, 454, 620, 893, 985
Bso31I GGTCTC 1 cut(s) 686
BsoBI CYCGRG 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 791
Bsp143I GATC 5 cut(s) 325, 742, 913, 1000, 1051
Bsp68I TCGCGA 1 cut(s) 555
BspACI CCGC 3 cut(s) 207, 210, 270
BspANI GGCC 6 cut(s) 37, 80, 454, 620, 893, 985
BspCNI CTCAG 1 cut(s) 985
BspFNI CGCG 1 cut(s) 555
BspLI GGNNCC 3 cut(s) 79, 636, 915
BspPI GGATC 5 cut(s) 333, 908, 921, 1008, 1046
BspTNI GGTCTC 1 cut(s) 686
BsrFI RCCGGY 1 cut(s) 80
BsrI ACTGG 2 cut(s) 696, 1022
BssAI RCCGGY 1 cut(s) 80
BssECI CCNNGG 2 cut(s) 490, 917
BssMI GATC 5 cut(s) 325, 742, 913, 1000, 1051
BssNI GRCGYC 1 cut(s) 463
BssT1I CCWWGG 2 cut(s) 490, 917
Bst2UI CCWGG 1 cut(s) 1056
Bst4CI ACNGT 4 cut(s) 62, 96, 356, 609
Bst6I CTCTTC 3 cut(s) 225, 243, 733
BstACI GRCGYC 1 cut(s) 463
BstC8I GCNNGC 4 cut(s) 199, 484, 648, 652
BstDEI CTNAG 2 cut(s) 578, 993
BstEII GGTNACC 1 cut(s) 803
BstF5I GGATG 3 cut(s) 86, 152, 486
BstFNI CGCG 1 cut(s) 555
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 2 cut(s) 197, 215
BstKTI GATC 5 cut(s) 328, 745, 916, 1003, 1054
BstMAI GTCTC 3 cut(s) 574, 686, 819
BstMBI GATC 5 cut(s) 325, 742, 913, 1000, 1051
BstMWI GCNNNNNNNGC 3 cut(s) 174, 207, 712
BstNI CCWGG 1 cut(s) 1056
BstPI GGTNACC 1 cut(s) 803
BstSCI CCNGG 1 cut(s) 1054
BstSFI CTRYAG 1 cut(s) 655
BstUI CGCG 1 cut(s) 555
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 4 cut(s) 325, 742, 913, 1051
BstYI RGATCY 4 cut(s) 325, 742, 913, 1051
BsuI GTATCC 1 cut(s) 1068
BsuRI GGCC 6 cut(s) 37, 80, 454, 620, 893, 985
BtgZI GCGATG 1 cut(s) 570
BtsCI GGATG 3 cut(s) 86, 152, 486
BtsI GCAGTG 2 cut(s) 184, 208
BtsIMutI CAGTG 4 cut(s) 184, 208, 605, 1044
BtuMI TCGCGA 1 cut(s) 555
Cac8I GCNNGC 4 cut(s) 199, 484, 648, 652
CfoI GCGC 2 cut(s) 197, 215
Cfr10I RCCGGY 1 cut(s) 80
Cfr13I GGNCC 3 cut(s) 35, 78, 503
CseI GACGC 1 cut(s) 704
CviAII CATG 1 cut(s) 1082
DdeI CTNAG 2 cut(s) 578, 993
DpnI GATC 5 cut(s) 327, 744, 915, 1002, 1053
DpnII GATC 5 cut(s) 325, 742, 913, 1000, 1051
EaeI YGGCCR 2 cut(s) 452, 891
Eam1104I CTCTTC 3 cut(s) 225, 243, 733
EarI CTCTTC 3 cut(s) 225, 243, 733
Eco130I CCWWGG 2 cut(s) 490, 917
Eco24I GRGCYC 1 cut(s) 791
Eco31I GGTCTC 1 cut(s) 686
Eco47I GGWCC 1 cut(s) 503
Eco57I CTGAAG 2 cut(s) 747, 1068
Eco88I CYCGRG 1 cut(s) 190
Eco91I GGTNACC 1 cut(s) 803
EcoO65I GGTNACC 1 cut(s) 803
EcoRI GAATTC 1 cut(s) 433
EcoRII CCWGG 1 cut(s) 1054
EcoT14I CCWWGG 2 cut(s) 490, 917
EcoT38I GRGCYC 1 cut(s) 791
ErhI CCWWGG 2 cut(s) 490, 917
FaeI CATG 1 cut(s) 1085
FalI AAGNNNNNCTT 2 cut(s) 901, 933
FatI CATG 1 cut(s) 1081
Fnu4HI GCNGC 3 cut(s) 208, 211, 270
FokI GGATG 3 cut(s) 73, 139, 473
FriOI GRGCYC 1 cut(s) 791
Fsp4HI GCNGC 3 cut(s) 208, 211, 270
FspBI CTAG 1 cut(s) 372
GlaI GCGC 2 cut(s) 196, 214
GluI GCNGC 3 cut(s) 208, 211, 270
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 6 cut(s) 37, 80, 454, 620, 893, 985
HapII CCGG 1 cut(s) 81
HgaI GACGC 1 cut(s) 704
HhaI GCGC 2 cut(s) 197, 215
Hin1I GRCGYC 1 cut(s) 463
Hin1II CATG 1 cut(s) 1085
Hin6I GCGC 2 cut(s) 195, 213
HinP1I GCGC 2 cut(s) 195, 213
HinfI GANTC 2 cut(s) 170, 1036
HpaII CCGG 1 cut(s) 81
HphI GGTGA 3 cut(s) 84, 815, 1076
Hpy166II GTNNAC 1 cut(s) 503
Hpy188I TCNGA 4 cut(s) 13, 340, 574, 747
Hpy188III TCNNGA 6 cut(s) 459, 554, 612, 632, 812, 959
Hpy8I GTNNAC 1 cut(s) 503
Hpy99I CGWCG 6 cut(s) 50, 53, 56, 59, 62, 115
HpyAV CCTTC 5 cut(s) 155, 662, 746, 818, 1002
HpyCH4III ACNGT 4 cut(s) 62, 96, 356, 609
HpyCH4IV ACGT 2 cut(s) 463, 562
HpyCH4V TGCA 5 cut(s) 482, 626, 819, 934, 967
HpyF10VI GCNNNNNNNGC 3 cut(s) 174, 207, 712
HpyF3I CTNAG 2 cut(s) 578, 993
HpySE526I ACGT 2 cut(s) 463, 562
Hsp92I GRCGYC 1 cut(s) 463
Hsp92II CATG 1 cut(s) 1085
HspAI GCGC 2 cut(s) 195, 213
Kzo9I GATC 5 cut(s) 325, 742, 913, 1000, 1051
LweI GCATC 2 cut(s) 495, 1078
MaeI CTAG 1 cut(s) 372
MaeII ACGT 2 cut(s) 463, 562
MaeIII GTNAC 3 cut(s) 803, 859, 1013
MalI GATC 5 cut(s) 327, 744, 915, 1002, 1053
MboI GATC 5 cut(s) 325, 742, 913, 1000, 1051
MflI RGATCY 4 cut(s) 325, 742, 913, 1051
MhlI GDGCHC 1 cut(s) 791
MlsI TGGCCA 2 cut(s) 454, 893
MluCI AATT 3 cut(s) 433, 523, 732
MluNI TGGCCA 2 cut(s) 454, 893
MlyI GAGTC 2 cut(s) 179, 1045
MmeI TCCRAC 2 cut(s) 180, 978
Mox20I TGGCCA 2 cut(s) 454, 893
MscI TGGCCA 2 cut(s) 454, 893
MseI TTAA 1 cut(s) 879
MslI CAYNNNNRTG 1 cut(s) 1086
Msp20I TGGCCA 2 cut(s) 454, 893
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 1056
MvaI CCWGG 1 cut(s) 1056
MvnI CGCG 1 cut(s) 555
MwoI GCNNNNNNNGC 3 cut(s) 174, 207, 712
NdeII GATC 5 cut(s) 325, 742, 913, 1000, 1051
NlaIII CATG 1 cut(s) 1085
NlaIV GGNNCC 3 cut(s) 79, 636, 915
NmuCI GTSAC 2 cut(s) 803, 859
NruI TCGCGA 1 cut(s) 555
PcsI WCGNNNNNNNCGW 5 cut(s) 51, 54, 116, 165, 559
PfoI TCCNGGA 1 cut(s) 1054
PkrI GCNGC 3 cut(s) 209, 212, 271
PleI GAGTC 2 cut(s) 178, 1044
PpsI GAGTC 2 cut(s) 178, 1044
PsiI TTATAA 1 cut(s) 903
Psp1406I AACGTT 1 cut(s) 562
Psp6I CCWGG 1 cut(s) 1054
PspEI GGTNACC 1 cut(s) 803
PspGI CCWGG 1 cut(s) 1054
PspN4I GGNNCC 3 cut(s) 79, 636, 915
PspPI GGNCC 3 cut(s) 35, 78, 503
PsrI GAACNNNNNNTAC 2 cut(s) 567, 599
PsuI RGATCY 4 cut(s) 325, 742, 913, 1051
RruI TCGCGA 1 cut(s) 555
RseI CAYNNNNRTG 1 cut(s) 1086
SaqAI TTAA 1 cut(s) 879
SatI GCNGC 3 cut(s) 208, 211, 270
Sau3AI GATC 5 cut(s) 325, 742, 913, 1000, 1051
Sau96I GGNCC 3 cut(s) 35, 78, 503
SchI GAGTC 2 cut(s) 179, 1045
ScrFI CCNGG 1 cut(s) 1056
SduI GDGCHC 1 cut(s) 791
SfaNI GCATC 2 cut(s) 495, 1078
SfcI CTRYAG 1 cut(s) 655
SinI GGWCC 1 cut(s) 503
SmiMI CAYNNNNRTG 1 cut(s) 1086
SmlI CTYRAG 1 cut(s) 792
SmoI CTYRAG 1 cut(s) 792
SpeI ACTAGT 1 cut(s) 371
Sse9I AATT 3 cut(s) 433, 523, 732
SsiI CCGC 3 cut(s) 207, 210, 270
SspI AATATT 1 cut(s) 835
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 1054
StyI CCWWGG 2 cut(s) 490, 917
TaaI ACNGT 4 cut(s) 62, 96, 356, 609
TaiI ACGT 2 cut(s) 466, 565
TaqI TCGA 3 cut(s) 496, 813, 1003
TasI AATT 3 cut(s) 433, 523, 732
TauI GCSGC 3 cut(s) 210, 213, 272
Tru1I TTAA 1 cut(s) 879
Tru9I TTAA 1 cut(s) 879
TscAI CASTG 4 cut(s) 184, 208, 612, 1051
TseFI GTSAC 2 cut(s) 803, 859
Tsp45I GTSAC 2 cut(s) 803, 859
TspDTI ATGAA 5 cut(s) 75, 141, 573, 765, 842
TspRI CASTG 4 cut(s) 184, 208, 612, 1051
VpaK11BI GGWCC 1 cut(s) 503
XapI RAATTY 2 cut(s) 433, 523
XspI CTAG 1 cut(s) 372
ZraI GACGTC 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.