pycom06g08110

Protein of unknown function (DUF3741)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006931
Physical Location & Seq
Reverse (-)
13189043 .. 13191558
2516 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1839 bp
ATGGCAAGGATGGCATCGCCAAGAAGCCCTATGCCGAGAAGTCCTATGCCGAAGAGCCCTATGCCAAAGAGCCCTATGGTTTGCGAAAAGTACGAGACAGGATGCATGTGGCATTTATATGGCCTTTTTAACTTTCGTCAAGGTCATTCGAATAAGAAGCTGCTTTCACACAGGAGGCATTTAAAGAGAATTGATGATGGAAATTTAAGGACCAAGCTTGATTTGCCTAACAAAATAGATGAGAAGTGCGAAAACATGGATGACGGAATGAAGAACAAAACTCAAACAGTTGATTCTGATATGGCAAGTAAAAGAAAGCTTAAGGGGGAAGAGTTGTCCACTGAGCTGCGGTTGAATAAGAACATTGCCGCTGATGAAGTGAAACATCTGCAATCTGATTCTAAATTTGTTGGTCCATTACCAAAGAATAATGGAAAGACCAGCAATAATCGTCAGAGATCTCATGATATACCTCTTCATGGTCTGAAGAATGACACTAAGCATCGAAAACCTTCTAATTCAACTTCAGCGGAAAAACCTTTAAATAAGCTCAGCTCAGCAGCATTGCCAGAAGTATTAAGCAAGGAGGTGCATAGAAAAAAGCGACGAGGATGTGGCTGTAAAAGTATTGATTACGTTAAGTATGGCCAGATTAATGAAAATAATCATCTGCCGGTACAGATGAATGCAGCGGATGCCATCATAAGTCAGAAATTCATTGATGGGGTGAACCACCAGTCTAAACAATTATCAGATGCGTTGCAGATTTTGAATTCGAATAAAGAATTATTTGTAGAACTCCTACAAGACCCGAATTCTCTGATAGTTAAACACATCGAGGACCCCCGAGAATCTCAGACAAGAAAACATCAGAGTAAATCTGTCTGTGAAGAGAACATTTCTGAATGTAGGACAAGTAAAGCAAGGCAATCTAAAGGTCCTCCCGGCATTCACAATTTAAATTCCTCCGATTTTTACCTATCTCAGGAAAGTAGTGATTCTGAATTTGCAGAGAGAATAGTAGTTTTAACAGCTGGCCCATCAAGATTGCAAAATTCTTCAGACGGTATCTATTCTTGCTCCTCTATGCAATCTTATTATAGCTTTAACAAAGATGGGGAAAGTGTGAGGCCTGCAAATTTTTCCCTCAGCCGTATGAAGAGGAAACTGAGACGTGCTATAGGGGTAAGCAGCAAAGAGCAGGACTCGACAACAATCAATGGTACACTTCAAATATCTCCTTGCCAAGGCTCAGAAGACTCTAAAGGAAAAGGTGTCAAGATCATGAGAAGAAATTCGCCTAGCACCAATAATTCTGCTGACAGTGGAGTAGTTAGCAAGTCTTCTTTTGATATTGAGAACAGAAACAACACAGGCAAGGTGAATGAATGTGAATCAAGCATTGGATGTGCAACTGCATCAACCAGTGGAACTGGCCTTCAAAACCCAAACATTTCACTTGTTAGCCAACCAAAGAAAAAGGAATCTGAGACTTTTGCGCAGTCTTTGGAGCATCTGTCAGAATTGTTACTGAAGGGAAACAAGGATAAGAACAACTTTGTAAGACGGACACCAAAAACCTGCGAAGGGGTGGCATCATTTCCTGAGGATGACTTTTTGCCTACACGCAGTCTGGAGAGGGACTGGGAAAATACATTTGTTGATGAACCAATGCGATTCTCACCCTACAGCAATTACCAACTGGTATATGAGAACAAGTCTAGGCTTCAGAAAGAAAAGAAAACCTGCTACTCGACTCCACTGAGGCAGGACGTAGAGGCCATGCCTGAGAATAAGAAACTTACTGCCCGTCGTGATCGATCAATTGCAAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3741 DUF4378

Protein Analysis

613

Amino Acids

68.8

Weight (kDa)

9.41

Isoelectric Point (pI)

63.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3741 PF12552 241 - 273 6.7e-14 Protein of unknown function (DUF3741)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1500
Acc36I ACCTGC 2 cut(s) 1589, 1754
AciI CCGC 4 cut(s) 349, 369, 530, 692
AcoI YGGCCR 1 cut(s) 646
AcuI CTGAAG 5 cut(s) 506, 510, 1044, 1553, 1712
AfaI GTAC 3 cut(s) 92, 678, 1225
AfiI CCNNNNNNNGG 4 cut(s) 479, 985, 1247, 1587
AflII CTTAAG 1 cut(s) 320
AgsI TTSAA 5 cut(s) 355, 522, 772, 1232, 1442
AjiI CACGTC 1 cut(s) 1175
AjuI GAANNNNNNNTTGG 2 cut(s) 1386, 1418
AluBI AGCT 8 cut(s) 160, 217, 319, 346, 550, 555, 1034, 1104
AluI AGCT 8 cut(s) 160, 217, 319, 346, 550, 555, 1034, 1104
Alw26I GTCTC 3 cut(s) 89, 1165, 1484
Ama87I CYCGRG 1 cut(s) 846
AoxI GGCC 6 cut(s) 121, 646, 1036, 1130, 1435, 1779
ApeKI GCWGC 5 cut(s) 160, 346, 560, 689, 1191
AseI ATTAAT 1 cut(s) 654
Asp700I GAANNNNTTC 2 cut(s) 511, 1294
AspLEI GCGC 1 cut(s) 1501
AspS9I GGNCC 5 cut(s) 210, 413, 841, 938, 1037
AsuC2I CCSGG 1 cut(s) 945
AsuHPI GGTGA 3 cut(s) 739, 1393, 1674
AsuII TTCGAA 2 cut(s) 149, 776
AvaI CYCGRG 1 cut(s) 846
AvaII GGWCC 4 cut(s) 210, 413, 841, 938
AxyI CCTNAGG 1 cut(s) 1605
BalI TGGCCA 1 cut(s) 648
BanII GRGCYC 2 cut(s) 59, 74
BbsI GAAGAC 2 cut(s) 1263, 1335
BbvCI CCTCAGC 1 cut(s) 1148
BbvI GCAGC 5 cut(s) 147, 333, 572, 701, 1203
BccI CCATC 6 cut(s) 4, 191, 707, 716, 1048, 1109
BceAI ACGGC 1 cut(s) 1137
BcnI CCSGG 1 cut(s) 945
BcoDI GTCTC 3 cut(s) 89, 1165, 1484
BfaI CTAG 2 cut(s) 1302, 1722
BfmI CTRYAG 2 cut(s) 1179, 1687
BfrI CTTAAG 1 cut(s) 320
BfuAI ACCTGC 2 cut(s) 1589, 1754
BglII AGATCT 1 cut(s) 458
BisI GCNGC 6 cut(s) 161, 347, 369, 561, 690, 1192
BlpI GCTNAGC 2 cut(s) 551, 556
BlsI GCNGC 6 cut(s) 162, 348, 370, 562, 691, 1193
Bme1390I CCNGG 1 cut(s) 945
Bme18I GGWCC 4 cut(s) 210, 413, 841, 938
BmeT110I CYCGRG 1 cut(s) 846
BmgBI CACGTC 1 cut(s) 1175
BmgT120I GGNCC 5 cut(s) 210, 413, 841, 938, 1037
BmiI GGNNCC 1 cut(s) 843
BmrFI CCNGG 1 cut(s) 945
BmrI ACTGGG 1 cut(s) 1654
BmsI GCATC 8 cut(s) 23, 92, 511, 685, 745, 1427, 1522, 1604
BmuI ACTGGG 1 cut(s) 1654
BpiI GAAGAC 2 cut(s) 1263, 1335
BplI GAGNNNNNCTC 2 cut(s) 1190, 1222
BpmI CTGGAG 1 cut(s) 1655
Bpu10I CCTNAGC 1 cut(s) 1148
Bpu1102I GCTNAGC 2 cut(s) 551, 556
Bpu14I TTCGAA 2 cut(s) 149, 776
BpuMI CCSGG 1 cut(s) 945
Bsa29I ATCGAT 1 cut(s) 1819
BsaJI CCNNGG 1 cut(s) 1246
BsaXI ACNNNNNCTCC 2 cut(s) 1502, 1532
Bsc4I CCNNNNNNNGG 4 cut(s) 479, 985, 1247, 1587
Bse118I RCCGGY 1 cut(s) 673
Bse1I ACTGG 5 cut(s) 736, 1425, 1438, 1649, 1707
Bse21I CCTNAGG 1 cut(s) 1605
Bse3DI GCAATG 2 cut(s) 363, 563
BseCI ATCGAT 1 cut(s) 1819
BseDI CCNNGG 1 cut(s) 1246
BseGI GGATG 7 cut(s) 15, 107, 265, 617, 700, 1412, 1615
BseLI CCNNNNNNNGG 4 cut(s) 479, 985, 1247, 1587
BseMI GCAATG 2 cut(s) 363, 563
BseNI ACTGG 5 cut(s) 736, 1425, 1438, 1649, 1707
BseRI GAGGAG 1 cut(s) 1072
BseXI GCAGC 5 cut(s) 147, 333, 572, 701, 1203
BshFI GGCC 6 cut(s) 123, 648, 1038, 1132, 1437, 1781
BshVI ATCGAT 1 cut(s) 1819
BsiHKCI CYCGRG 1 cut(s) 846
BsiSI CCGG 2 cut(s) 674, 945
BslFI GGGAC 1 cut(s) 1655
BslI CCNNNNNNNGG 4 cut(s) 479, 985, 1247, 1587
BsmAI GTCTC 3 cut(s) 89, 1165, 1484
BsmBI CGTCTC 1 cut(s) 1165
BsmFI GGGAC 1 cut(s) 1655
BsmI GAATGC 2 cut(s) 691, 948
BsnI GGCC 6 cut(s) 123, 648, 1038, 1132, 1437, 1781
BsoBI CYCGRG 1 cut(s) 846
Bsp119I TTCGAA 2 cut(s) 149, 776
Bsp1286I GDGCHC 2 cut(s) 59, 74
Bsp143I GATC 4 cut(s) 458, 1281, 1816, 1820
Bsp1720I GCTNAGC 2 cut(s) 551, 556
BspACI CCGC 4 cut(s) 349, 369, 530, 692
BspANI GGCC 6 cut(s) 123, 648, 1038, 1132, 1437, 1781
BspDI ATCGAT 1 cut(s) 1819
BspHI TCATGA 2 cut(s) 463, 1284
BspLI GGNNCC 1 cut(s) 843
BspMI ACCTGC 2 cut(s) 1589, 1754
BspQI GCTCTTC 1 cut(s) 47
BspT104I TTCGAA 2 cut(s) 149, 776
BspTI CTTAAG 1 cut(s) 320
BsrDI GCAATG 2 cut(s) 363, 563
BsrFI RCCGGY 1 cut(s) 673
BsrI ACTGG 5 cut(s) 736, 1425, 1438, 1649, 1707
BssAI RCCGGY 1 cut(s) 673
BssECI CCNNGG 1 cut(s) 1246
BssMI GATC 4 cut(s) 458, 1281, 1816, 1820
BssT1I CCWWGG 1 cut(s) 1246
Bst4CI ACNGT 3 cut(s) 289, 1067, 1325
Bst6I CTCTTC 5 cut(s) 47, 324, 480, 885, 1154
BstAFI CTTAAG 1 cut(s) 320
BstAPI GCANNNNNTGC 1 cut(s) 695
BstBI TTCGAA 2 cut(s) 149, 776
BstC8I GCNNGC 2 cut(s) 1036, 1134
BstENI CCTNNNNNAGG 2 cut(s) 983, 1245
BstF5I GGATG 7 cut(s) 15, 107, 265, 617, 700, 1412, 1615
BstHHI GCGC 1 cut(s) 1501
BstKTI GATC 4 cut(s) 461, 1284, 1819, 1823
BstMAI GTCTC 3 cut(s) 89, 1165, 1484
BstMBI GATC 4 cut(s) 458, 1281, 1816, 1820
BstMWI GCNNNNNNNGC 3 cut(s) 11, 223, 695
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 943
BstSFI CTRYAG 2 cut(s) 1179, 1687
BstV1I GCAGC 5 cut(s) 147, 333, 572, 701, 1203
BstV2I GAAGAC 2 cut(s) 1263, 1335
BstX2I RGATCY 1 cut(s) 458
BstYI RGATCY 1 cut(s) 458
Bsu15I ATCGAT 1 cut(s) 1819
Bsu36I CCTNAGG 1 cut(s) 1605
BsuRI GGCC 6 cut(s) 123, 648, 1038, 1132, 1437, 1781
BsuTUI ATCGAT 1 cut(s) 1819
BtrI CACGTC 1 cut(s) 1175
BtsCI GGATG 7 cut(s) 15, 107, 265, 617, 700, 1412, 1615
BtsIMutI CAGTG 4 cut(s) 339, 1330, 1432, 1760
BveI ACCTGC 2 cut(s) 1589, 1754
Cac8I GCNNGC 2 cut(s) 1036, 1134
CciI TCATGA 2 cut(s) 463, 1284
CfoI GCGC 1 cut(s) 1501
Cfr10I RCCGGY 1 cut(s) 673
Cfr13I GGNCC 5 cut(s) 210, 413, 841, 938, 1037
ClaI ATCGAT 1 cut(s) 1819
Csp6I GTAC 3 cut(s) 91, 677, 1224
CviAII CATG 6 cut(s) 106, 256, 464, 479, 1285, 1783
CviQI GTAC 3 cut(s) 91, 677, 1224
DpnI GATC 4 cut(s) 460, 1283, 1818, 1822
DpnII GATC 4 cut(s) 458, 1281, 1816, 1820
DraI TTTAAA 3 cut(s) 183, 543, 960
EaeI YGGCCR 1 cut(s) 646
Eam1104I CTCTTC 5 cut(s) 47, 324, 480, 885, 1154
EarI CTCTTC 5 cut(s) 47, 324, 480, 885, 1154
Eco130I CCWWGG 1 cut(s) 1246
Eco147I AGGCCT 1 cut(s) 1132
Eco24I GRGCYC 2 cut(s) 59, 74
Eco47I GGWCC 4 cut(s) 210, 413, 841, 938
Eco57I CTGAAG 5 cut(s) 506, 510, 1044, 1553, 1712
Eco81I CCTNAGG 1 cut(s) 1605
Eco88I CYCGRG 1 cut(s) 846
EcoNI CCTNNNNNAGG 2 cut(s) 983, 1245
EcoO109I RGGNCCY 2 cut(s) 841, 938
EcoRI GAATTC 2 cut(s) 772, 814
EcoT14I CCWWGG 1 cut(s) 1246
EcoT22I ATGCAT 1 cut(s) 107
EcoT38I GRGCYC 2 cut(s) 59, 74
ErhI CCWWGG 1 cut(s) 1246
Esp3I CGTCTC 1 cut(s) 1165
FaeI CATG 6 cut(s) 109, 259, 467, 482, 1288, 1786
FalI AAGNNNNNCTT 2 cut(s) 1541, 1573
FaqI GGGAC 1 cut(s) 1655
FatI CATG 6 cut(s) 105, 255, 463, 478, 1284, 1782
Fnu4HI GCNGC 6 cut(s) 161, 347, 369, 561, 690, 1192
FokI GGATG 7 cut(s) 22, 114, 272, 624, 707, 1419, 1622
FriOI GRGCYC 2 cut(s) 59, 74
Fsp4HI GCNGC 6 cut(s) 161, 347, 369, 561, 690, 1192
FspBI CTAG 2 cut(s) 1302, 1722
FspI TGCGCA 1 cut(s) 1500
GlaI GCGC 1 cut(s) 1500
GluI GCNGC 6 cut(s) 161, 347, 369, 561, 690, 1192
GsuI CTGGAG 1 cut(s) 1655
HaeIII GGCC 6 cut(s) 123, 648, 1038, 1132, 1437, 1781
HapII CCGG 2 cut(s) 674, 945
HhaI GCGC 1 cut(s) 1501
Hin1II CATG 6 cut(s) 109, 259, 467, 482, 1288, 1786
Hin6I GCGC 1 cut(s) 1499
HinP1I GCGC 1 cut(s) 1499
HindIII AAGCTT 2 cut(s) 215, 317
HpaII CCGG 2 cut(s) 674, 945
HphI GGTGA 3 cut(s) 739, 1393, 1674
Hpy166II GTNNAC 3 cut(s) 339, 730, 1226
Hpy188III TCNNGA 9 cut(s) 464, 986, 1044, 1279, 1285, 1604, 1634, 1814, 1836
Hpy8I GTNNAC 3 cut(s) 339, 730, 1226
Hpy99I CGWCG 2 cut(s) 609, 1815
HpyAV CCTTC 4 cut(s) 522, 1448, 1528, 1580
HpyCH4III ACNGT 3 cut(s) 289, 1067, 1325
HpyCH4IV ACGT 3 cut(s) 636, 1174, 1773
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 223, 695
HpySE526I ACGT 3 cut(s) 636, 1174, 1773
Hsp92II CATG 6 cut(s) 109, 259, 467, 482, 1288, 1786
HspAI GCGC 1 cut(s) 1499
Kzo9I GATC 4 cut(s) 458, 1281, 1816, 1820
LguI GCTCTTC 1 cut(s) 47
LmnI GCTCC 2 cut(s) 1085, 1510
Lsp1109I GCAGC 5 cut(s) 147, 333, 572, 701, 1203
LweI GCATC 8 cut(s) 23, 92, 511, 685, 745, 1427, 1522, 1604
MaeI CTAG 2 cut(s) 1302, 1722
MaeII ACGT 3 cut(s) 636, 1174, 1773
MaeIII GTNAC 1 cut(s) 1527
MalI GATC 4 cut(s) 460, 1283, 1818, 1822
MboI GATC 4 cut(s) 458, 1281, 1816, 1820
MfeI CAATTG 1 cut(s) 1824
MflI RGATCY 1 cut(s) 458
MhlI GDGCHC 2 cut(s) 59, 74
MlsI TGGCCA 1 cut(s) 648
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 3 cut(s) 1199, 1253, 1750
Mox20I TGGCCA 1 cut(s) 648
Mph1103I ATGCAT 1 cut(s) 107
MroXI GAANNNNTTC 2 cut(s) 511, 1294
MscI TGGCCA 1 cut(s) 648
MslI CAYNNNNRTG 1 cut(s) 117
Msp20I TGGCCA 1 cut(s) 648
MspA1I CMGCKG 4 cut(s) 371, 530, 692, 1034
MspCI CTTAAG 1 cut(s) 320
MspI CCGG 2 cut(s) 674, 945
MspR9I CCNGG 1 cut(s) 945
MunI CAATTG 1 cut(s) 1824
Mva1269I GAATGC 2 cut(s) 691, 948
MwoI GCNNNNNNNGC 3 cut(s) 11, 223, 695
NciI CCSGG 1 cut(s) 945
NdeII GATC 4 cut(s) 458, 1281, 1816, 1820
NlaIII CATG 6 cut(s) 109, 259, 467, 482, 1288, 1786
NlaIV GGNNCC 1 cut(s) 843
NmeAIII GCCGAG 1 cut(s) 60
NsbI TGCGCA 1 cut(s) 1500
NsiI ATGCAT 1 cut(s) 107
NspI RCATGY 1 cut(s) 109
NspV TTCGAA 2 cut(s) 149, 776
PagI TCATGA 2 cut(s) 463, 1284
PceI AGGCCT 1 cut(s) 1132
PciSI GCTCTTC 1 cut(s) 47
PctI GAATGC 2 cut(s) 691, 948
PdmI GAANNNNTTC 2 cut(s) 511, 1294
PfeI GAWTC 7 cut(s) 293, 398, 851, 998, 1394, 1484, 1677
PkrI GCNGC 6 cut(s) 162, 348, 370, 562, 691, 1193
PleI GAGTC 3 cut(s) 1199, 1253, 1750
PpsI GAGTC 3 cut(s) 1199, 1253, 1750
PpuMI RGGWCCY 2 cut(s) 841, 938
PshBI ATTAAT 1 cut(s) 654
Psp5II RGGWCCY 2 cut(s) 841, 938
PspN4I GGNNCC 1 cut(s) 843
PspPI GGNCC 5 cut(s) 210, 413, 841, 938, 1037
PspPPI RGGWCCY 2 cut(s) 841, 938
PsuI RGATCY 1 cut(s) 458
PvuII CAGCTG 1 cut(s) 1034
RsaI GTAC 3 cut(s) 92, 678, 1225
RsaNI GTAC 3 cut(s) 91, 677, 1224
RseI CAYNNNNRTG 1 cut(s) 117
SapI GCTCTTC 1 cut(s) 47
SatI GCNGC 6 cut(s) 161, 347, 369, 561, 690, 1192
Sau3AI GATC 4 cut(s) 458, 1281, 1816, 1820
Sau96I GGNCC 5 cut(s) 210, 413, 841, 938, 1037
SchI GAGTC 3 cut(s) 1199, 1253, 1750
ScrFI CCNGG 1 cut(s) 945
SduI GDGCHC 2 cut(s) 59, 74
SfaNI GCATC 8 cut(s) 23, 92, 511, 685, 745, 1427, 1522, 1604
SfcI CTRYAG 2 cut(s) 1179, 1687
SfuI TTCGAA 2 cut(s) 149, 776
SinI GGWCC 4 cut(s) 210, 413, 841, 938
SmiI ATTTAAAT 1 cut(s) 960
SmiMI CAYNNNNRTG 1 cut(s) 117
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SseBI AGGCCT 1 cut(s) 1132
SsiI CCGC 4 cut(s) 349, 369, 530, 692
SspMI CTAG 2 cut(s) 1302, 1722
StuI AGGCCT 1 cut(s) 1132
StyD4I CCNGG 1 cut(s) 943
StyI CCWWGG 1 cut(s) 1246
SwaI ATTTAAAT 1 cut(s) 960
TaaI ACNGT 3 cut(s) 289, 1067, 1325
TaiI ACGT 3 cut(s) 639, 1177, 1776
TaqI TCGA 7 cut(s) 149, 505, 776, 837, 1208, 1754, 1819
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 7 cut(s) 293, 398, 851, 998, 1394, 1484, 1677
TscAI CASTG 4 cut(s) 346, 1330, 1432, 1767
TseI GCWGC 5 cut(s) 160, 346, 560, 689, 1191
TspDTI ATGAA 9 cut(s) 284, 390, 467, 672, 698, 706, 1172, 1401, 1680
TspGWI ACGGA 2 cut(s) 279, 1582
TspRI CASTG 4 cut(s) 346, 1330, 1432, 1767
Vha464I CTTAAG 1 cut(s) 320
VpaK11BI GGWCC 4 cut(s) 210, 413, 841, 938
VspI ATTAAT 1 cut(s) 654
XagI CCTNNNNNAGG 2 cut(s) 983, 1245
XceI RCATGY 1 cut(s) 109
XmnI GAANNNNTTC 2 cut(s) 511, 1294
XspI CTAG 2 cut(s) 1302, 1722
Zsp2I ATGCAT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.