pycom06g08130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012612
Physical Location & Seq
Forward (+)
13209063 .. 13210466
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1404 bp
ATGGGTGATGAGGTTAAACAGCAAAGGTGGGAGTTCAGGAGGAAGGACAACGACAATGATTCTTCAAGTGACGATTCTAAATCAAGTAGTGAGGCGGCTCACGAGAAGTGTAAACTGGTTTCCGGTTTGATTTCACTTGAAAACGATGAAGCAGTCGATAACTGCAGAGCTCAGCAAACTGGCATGTTCATTCCTTGTGGGGAAGATCAAAACATGTCAAAGAAAGCCAAAAGGGTGCACCTTAGGAGTAAGAAGAATAGTAGAAAAAAGAGTAAAACTGGCGTTTACAATTCTGCTGCTGCTTTGGATGAACTTAAAATCTTCAAGGAGTCCCTATTAGAGAATCTTAAAGTGACGAGAGAAAATTTGCTTTCATGGATGAGGAAGGAAATGCAAAAACTGGTGGATGATGATGCCGCCCCGCGACTTGAACGGAGAGAAGGAAGTGCTGGAGGGAAAAATGTTCGAGTACAACATGGAAATGACTTTGAGAAGAACACCATCTATAAAGAGAACATACAGGTGAATAACGAAAGAAAAAATGAGGATAACATTCAGCATCAAAACAACTTTGGGAAGAATGTCCCAGTGCATCGAGAAAGCAACTCTAAGGAGAATGTATTTGCACACCATCACAACAATGTGAAGGAGACGGTCCAAGTGCAACATCTGGATGAAAAAGTAAAATTTCAAGTGAACTATCGGAACAACTTTGAGTTCGGCACAAAACCTCGACAATGCGATATTGAATCTTTAGGGAGGAGTGTTAAAAGCAATACAGCAGCTGGTTCTGATAATTTGTATCATGTTAAGCCAAATTTTCAATCCTATTCCACAGACAAGAACGTCCTGATGCAGCATCCCAGTCAAGTACCTTCTTCGATGTATTTGACATTGCCCACCGTGTTAACTAAGCCACGCGGTGAGAACTATACGCTCAGTACTTCATCAAGCAATTATATTGGAACACCATTTGACATAAACAAGCTAGATGTGAACTCTGTGAAGGTAAATCCAATGCTCAAAGTAAAGTGTGGAAAATTATCCGGCATGCCGCAGCTCGAAAGGGATGGAAGCTTTCCTCAAATAAGGTCGAGAAATGCGAATTGTTTTGATAAACAACGCATCCCAAGTTCAAGCATTGGAAATGGATTCCCAGTTCCATTTCATCAGGGTGATGATATCGGTCTTAGCATTCCGAGCCAAGTAAGTTCCGAATACTTGCCCCGGGAAGAAAAGAACATAATGGGTTTAAAGATGGAAGAAGGAGCTTCAAGCTTCTTCGGCGGAAGCTATACTAAATCAGAAGACTATTTCGCAAACAATGTCTACCGACACTCCATGAGTAAAGCTCATGGTACACACAAGGACCTTCAAGAGGGCTATTTGTTCCCAAAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

468

Amino Acids

53.01

Weight (kDa)

8.99

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 845
AccI GTMKAC 1 cut(s) 1329
AccII CGCG 2 cut(s) 424, 921
AciI CCGC 6 cut(s) 95, 417, 422, 921, 1055, 1287
AcsI RAATTY 3 cut(s) 364, 686, 817
AdeI CACNNNGTG 1 cut(s) 923
AfaI GTAC 4 cut(s) 471, 873, 943, 1360
AfiI CCNNNNNNNGG 1 cut(s) 1378
AflIII ACRYGT 1 cut(s) 213
AloI GAACNNNNNNTCC 2 cut(s) 1143, 1175
AluBI AGCT 9 cut(s) 170, 785, 988, 1060, 1077, 1271, 1278, 1293, 1352
AluI AGCT 9 cut(s) 170, 785, 988, 1060, 1077, 1271, 1278, 1293, 1352
Alw21I GWGCWC 2 cut(s) 172, 240
Alw26I GTCTC 1 cut(s) 644
Alw44I GTGCAC 1 cut(s) 236
AlwNI CAGNNNCTG 1 cut(s) 785
Ama87I CYCGRG 1 cut(s) 1227
ApaLI GTGCAC 1 cut(s) 236
ApeKI GCWGC 5 cut(s) 296, 299, 782, 856, 1057
ApoI RAATTY 3 cut(s) 364, 686, 817
AspS9I GGNCC 2 cut(s) 655, 1369
AsuC2I CCSGG 2 cut(s) 1228, 1229
AsuHPI GGTGA 4 cut(s) 17, 535, 935, 1187
AvaI CYCGRG 1 cut(s) 1227
AvaII GGWCC 2 cut(s) 655, 1369
AxyI CCTNAGG 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 240
BanII GRGCYC 1 cut(s) 172
BauI CACGAG 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 1314
Bbv12I GWGCWC 2 cut(s) 172, 240
BbvI GCAGC 5 cut(s) 283, 286, 794, 868, 1069
BccI CCATC 4 cut(s) 509, 639, 1064, 1252
BcnI CCSGG 2 cut(s) 1228, 1229
BcoDI GTCTC 1 cut(s) 644
BfaI CTAG 1 cut(s) 989
BfmI CTRYAG 1 cut(s) 163
BisI GCNGC 8 cut(s) 96, 297, 300, 417, 783, 857, 1055, 1058
BlpI GCTNAGC 1 cut(s) 171
BlsI GCNGC 8 cut(s) 97, 298, 301, 418, 784, 858, 1056, 1059
BmcAI AGTACT 1 cut(s) 943
Bme1390I CCNGG 2 cut(s) 1228, 1229
Bme18I GGWCC 2 cut(s) 655, 1369
BmeT110I CYCGRG 1 cut(s) 1227
BmgT120I GGNCC 2 cut(s) 655, 1369
BmrFI CCNGG 2 cut(s) 1228, 1229
BmrI ACTGGG 3 cut(s) 581, 858, 1151
BmsI GCATC 6 cut(s) 403, 568, 601, 843, 868, 1134
BmuI ACTGGG 3 cut(s) 581, 858, 1151
BpiI GAAGAC 1 cut(s) 1314
BplI GAGNNNNNCTC 2 cut(s) 1336, 1368
BpmI CTGGAG 1 cut(s) 471
Bpu1102I GCTNAGC 1 cut(s) 171
BpuMI CCSGG 2 cut(s) 1228, 1229
BsaJI CCNNGG 2 cut(s) 1226, 1227
BsaWI WCCGGW 1 cut(s) 122
BsaXI ACNNNNNCTCC 2 cut(s) 1322, 1352
Bsc4I CCNNNNNNNGG 1 cut(s) 1378
Bse1I ACTGG 7 cut(s) 120, 184, 283, 405, 587, 864, 1157
Bse21I CCTNAGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 893
BseDI CCNNGG 2 cut(s) 1226, 1227
BseGI GGATG 7 cut(s) 313, 384, 412, 679, 859, 1075, 1125
BseLI CCNNNNNNNGG 1 cut(s) 1378
BseMI GCAATG 1 cut(s) 893
BseMII CTCAG 2 cut(s) 185, 952
BseNI ACTGG 7 cut(s) 120, 184, 283, 405, 587, 864, 1157
BseRI GAGGAG 1 cut(s) 775
BseSI GKGCMC 1 cut(s) 240
BseXI GCAGC 5 cut(s) 283, 286, 794, 868, 1069
Bsh1236I CGCG 2 cut(s) 424, 921
BsiHKAI GWGCWC 2 cut(s) 172, 240
BsiHKCI CYCGRG 1 cut(s) 1227
BsiSI CCGG 3 cut(s) 123, 1047, 1228
BslFI GGGAC 2 cut(s) 316, 569
BslI CCNNNNNNNGG 1 cut(s) 1378
BsmAI GTCTC 1 cut(s) 644
BsmBI CGTCTC 1 cut(s) 644
BsmFI GGGAC 2 cut(s) 316, 569
BsmI GAATGC 1 cut(s) 1194
BsoBI CYCGRG 1 cut(s) 1227
Bsp1286I GDGCHC 2 cut(s) 172, 240
Bsp143I GATC 1 cut(s) 205
Bsp1720I GCTNAGC 1 cut(s) 171
BspACI CCGC 6 cut(s) 95, 417, 422, 921, 1055, 1287
BspCNI CTCAG 2 cut(s) 184, 951
BspFNI CGCG 2 cut(s) 424, 921
BspMAI CTGCAG 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 893
BsrI ACTGG 7 cut(s) 120, 184, 283, 405, 587, 864, 1157
BssECI CCNNGG 2 cut(s) 1226, 1227
BssMI GATC 1 cut(s) 205
BssSI CACGAG 1 cut(s) 101
Bst2BI CACGAG 1 cut(s) 101
Bst4CI ACNGT 2 cut(s) 655, 904
BstC8I GCNNGC 1 cut(s) 1052
BstDEI CTNAG 6 cut(s) 171, 242, 609, 912, 938, 1190
BstENI CCTNNNNNAGG 1 cut(s) 1376
BstF5I GGATG 7 cut(s) 313, 384, 412, 679, 859, 1075, 1125
BstFNI CGCG 2 cut(s) 424, 921
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 1 cut(s) 644
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 2 cut(s) 1200, 1284
BstNSI RCATGY 3 cut(s) 187, 217, 1054
BstSCI CCNGG 2 cut(s) 1226, 1227
BstSFI CTRYAG 1 cut(s) 163
BstSLI GKGCMC 1 cut(s) 240
BstUI CGCG 2 cut(s) 424, 921
BstV1I GCAGC 5 cut(s) 283, 286, 794, 868, 1069
BstV2I GAAGAC 1 cut(s) 1314
Bsu36I CCTNAGG 1 cut(s) 242
BtsCI GGATG 7 cut(s) 313, 384, 412, 679, 859, 1075, 1125
BtsIMutI CAGTG 1 cut(s) 594
Cac8I GCNNGC 1 cut(s) 1052
CaiI CAGNNNCTG 1 cut(s) 785
Cfr13I GGNCC 2 cut(s) 655, 1369
Cfr9I CCCGGG 1 cut(s) 1227
Csp6I GTAC 4 cut(s) 470, 872, 942, 1359
CviAII CATG 8 cut(s) 184, 214, 375, 476, 806, 1051, 1342, 1355
CviQI GTAC 4 cut(s) 470, 872, 942, 1359
DdeI CTNAG 6 cut(s) 171, 242, 609, 912, 938, 1190
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
DraI TTTAAA 1 cut(s) 1254
DraIII CACNNNGTG 1 cut(s) 923
DrdI GACNNNNNNGTC 1 cut(s) 845
DseDI GACNNNNNNGTC 1 cut(s) 845
EciI GGCGGA 1 cut(s) 1302
Ecl136II GAGCTC 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 172
Eco32I GATATC 1 cut(s) 1183
Eco47I GGWCC 2 cut(s) 655, 1369
Eco53kI GAGCTC 1 cut(s) 170
Eco81I CCTNAGG 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 1227
EcoICRI GAGCTC 1 cut(s) 170
EcoNI CCTNNNNNAGG 1 cut(s) 1376
EcoO109I RGGNCCY 1 cut(s) 1369
EcoRV GATATC 1 cut(s) 1183
EcoT38I GRGCYC 1 cut(s) 172
Esp3I CGTCTC 1 cut(s) 644
FaeI CATG 8 cut(s) 187, 217, 378, 479, 809, 1054, 1345, 1358
FaqI GGGAC 2 cut(s) 316, 569
FatI CATG 8 cut(s) 183, 213, 374, 475, 805, 1050, 1341, 1354
FauI CCCGC 1 cut(s) 429
FblI GTMKAC 1 cut(s) 1329
Fnu4HI GCNGC 8 cut(s) 96, 297, 300, 417, 783, 857, 1055, 1058
FokI GGATG 7 cut(s) 320, 391, 419, 686, 846, 1082, 1112
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 8 cut(s) 96, 297, 300, 417, 783, 857, 1055, 1058
FspBI CTAG 1 cut(s) 989
GluI GCNGC 8 cut(s) 96, 297, 300, 417, 783, 857, 1055, 1058
GsuI CTGGAG 1 cut(s) 471
HapII CCGG 3 cut(s) 123, 1047, 1228
Hin1II CATG 8 cut(s) 187, 217, 378, 479, 809, 1054, 1345, 1358
HincII GTYRAC 1 cut(s) 909
HindII GTYRAC 1 cut(s) 909
HindIII AAGCTT 2 cut(s) 1075, 1276
HinfI GANTC 6 cut(s) 59, 74, 329, 343, 749, 1152
HpaI GTTAAC 1 cut(s) 909
HpaII CCGG 3 cut(s) 123, 1047, 1228
HphI GGTGA 4 cut(s) 17, 535, 935, 1187
Hpy166II GTNNAC 8 cut(s) 113, 238, 286, 697, 909, 997, 1330, 1361
Hpy188I TCNGA 5 cut(s) 705, 793, 1200, 1216, 1306
Hpy188III TCNNGA 7 cut(s) 37, 101, 596, 671, 850, 1095, 1376
Hpy8I GTNNAC 8 cut(s) 113, 238, 286, 697, 909, 997, 1330, 1361
HpyAV CCTTC 8 cut(s) 37, 379, 434, 640, 885, 1000, 1259, 1382
HpyCH4III ACNGT 2 cut(s) 655, 904
HpyCH4IV ACGT 1 cut(s) 846
HpyCH4V TGCA 7 cut(s) 165, 238, 394, 592, 626, 664, 856
HpyF10VI GCNNNNNNNGC 2 cut(s) 1200, 1284
HpyF3I CTNAG 6 cut(s) 171, 242, 609, 912, 938, 1190
HpySE526I ACGT 1 cut(s) 846
Hsp92II CATG 8 cut(s) 187, 217, 378, 479, 809, 1054, 1345, 1358
KspAI GTTAAC 1 cut(s) 909
Kzo9I GATC 1 cut(s) 205
LmnI GCTCC 1 cut(s) 1268
Lsp1109I GCAGC 5 cut(s) 283, 286, 794, 868, 1069
LweI GCATC 6 cut(s) 403, 568, 601, 843, 868, 1134
MaeI CTAG 1 cut(s) 989
MaeII ACGT 1 cut(s) 846
MaeIII GTNAC 2 cut(s) 68, 352
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MhlI GDGCHC 2 cut(s) 172, 240
MluCI AATT 8 cut(s) 289, 364, 686, 796, 817, 955, 1040, 1105
MlyI GAGTC 1 cut(s) 338
MseI TTAA 7 cut(s) 15, 315, 348, 768, 810, 908, 1253
MslI CAYNNNNRTG 5 cut(s) 480, 521, 639, 672, 1173
MspA1I CMGCKG 1 cut(s) 785
MspI CCGG 3 cut(s) 123, 1047, 1228
MspR9I CCNGG 2 cut(s) 1228, 1229
Mva1269I GAATGC 1 cut(s) 1194
MvnI CGCG 2 cut(s) 424, 921
MwoI GCNNNNNNNGC 2 cut(s) 1200, 1284
NciI CCSGG 2 cut(s) 1228, 1229
NdeII GATC 1 cut(s) 205
NlaIII CATG 8 cut(s) 187, 217, 378, 479, 809, 1054, 1345, 1358
NmuCI GTSAC 2 cut(s) 68, 352
NspI RCATGY 3 cut(s) 187, 217, 1054
PaeI GCATGC 1 cut(s) 1054
PciI ACATGT 1 cut(s) 213
PcsI WCGNNNNNNNCGW 1 cut(s) 1100
PctI GAATGC 1 cut(s) 1194
PfeI GAWTC 5 cut(s) 59, 74, 343, 749, 1152
PkrI GCNGC 8 cut(s) 97, 298, 301, 418, 784, 858, 1056, 1059
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 1369
PscI ACATGT 1 cut(s) 213
Psp124BI GAGCTC 1 cut(s) 172
Psp5II RGGWCCY 1 cut(s) 1369
PspPI GGNCC 2 cut(s) 655, 1369
PspPPI RGGWCCY 1 cut(s) 1369
PstI CTGCAG 1 cut(s) 167
PstNI CAGNNNCTG 1 cut(s) 785
PvuII CAGCTG 1 cut(s) 785
RsaI GTAC 4 cut(s) 471, 873, 943, 1360
RsaNI GTAC 4 cut(s) 470, 872, 942, 1359
RseI CAYNNNNRTG 5 cut(s) 480, 521, 639, 672, 1173
SacI GAGCTC 1 cut(s) 172
SaqAI TTAA 7 cut(s) 15, 315, 348, 768, 810, 908, 1253
SatI GCNGC 8 cut(s) 96, 297, 300, 417, 783, 857, 1055, 1058
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 2 cut(s) 655, 1369
ScaI AGTACT 1 cut(s) 943
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 2 cut(s) 1228, 1229
SduI GDGCHC 2 cut(s) 172, 240
SfaNI GCATC 6 cut(s) 403, 568, 601, 843, 868, 1134
SfcI CTRYAG 1 cut(s) 163
SinI GGWCC 2 cut(s) 655, 1369
SmaI CCCGGG 1 cut(s) 1229
SmiMI CAYNNNNRTG 5 cut(s) 480, 521, 639, 672, 1173
SphI GCATGC 1 cut(s) 1054
Sse9I AATT 8 cut(s) 289, 364, 686, 796, 817, 955, 1040, 1105
SsiI CCGC 6 cut(s) 95, 417, 422, 921, 1055, 1287
SspMI CTAG 1 cut(s) 989
SstI GAGCTC 1 cut(s) 172
StyD4I CCNGG 2 cut(s) 1226, 1227
TaaI ACNGT 2 cut(s) 655, 904
TaiI ACGT 1 cut(s) 849
TaqI TCGA 7 cut(s) 156, 466, 595, 733, 881, 1062, 1094
TaqII GACCGA 1 cut(s) 1175
TasI AATT 8 cut(s) 289, 364, 686, 796, 817, 955, 1040, 1105
TatI WGTACW 2 cut(s) 469, 941
TauI GCSGC 3 cut(s) 98, 419, 1057
TfiI GAWTC 5 cut(s) 59, 74, 343, 749, 1152
Tru1I TTAA 7 cut(s) 15, 315, 348, 768, 810, 908, 1253
Tru9I TTAA 7 cut(s) 15, 315, 348, 768, 810, 908, 1253
TscAI CASTG 1 cut(s) 594
TseFI GTSAC 2 cut(s) 68, 352
TseI GCWGC 5 cut(s) 296, 299, 782, 856, 1057
Tsp45I GTSAC 2 cut(s) 68, 352
TspDTI ATGAA 7 cut(s) 162, 178, 324, 363, 690, 936, 1157
TspGWI ACGGA 1 cut(s) 448
TspMI CCCGGG 1 cut(s) 1227
TspRI CASTG 1 cut(s) 594
VneI GTGCAC 1 cut(s) 236
VpaK11BI GGWCC 2 cut(s) 655, 1369
XagI CCTNNNNNAGG 1 cut(s) 1376
XapI RAATTY 3 cut(s) 364, 686, 817
XceI RCATGY 3 cut(s) 187, 217, 1054
XmaI CCCGGG 1 cut(s) 1227
XmiI GTMKAC 1 cut(s) 1329
XspI CTAG 1 cut(s) 989
ZrmI AGTACT 1 cut(s) 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.