RLG00000003937

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012612
Physical Location & Seq
Forward (+)
54449536 .. 54451170
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003937

Sequence Viewer

Length: 1635 bp
ATGGGTGGTGACCTGAGGTGGGAGTTTAGGAGGAAGGACAATGGCGATGAGTCTTCAAGTGATGAATCTAAATCAAGTAGTGAGGCGGCTCACGAGCAGCATAAACTGGTTTCCTCTTTGATTTCGGTTGAAAATGATGAAGAAATTGGAACTCATAGAGTCAAGCAGAATGTCACGAGCAATCCTCATACTGAGGTTAAAATTATGTCTGGGAAAGGCAAAAATGTGCACTCACAAAGGAGTGGAAAGGAAAGTTCAATTCATGATGGCACAGAGAGGGATTCTCATGAAGAAGGCAGTAGAAAAAAGAGTAAAACCAGTATTTATGAGCCTGCTGCCATGGATGAGATTAAGAGATTCATGGAGTCCTTATTAGAGGATCTTAGAGTGACAAGAGAAAATTTGTTTTCATGGATGAGGCAGGAAATGCAAAAATTGGTGATGGATGATACTCTTCCACCTCTTGAACGGAGAGAACAAAGTTTTGGAGAGAAAAATCTCCTAGTGCAGCATGGAGAAATCGTTGAGAACACCACCTCTAAGGAGAATAGACAAGTGAACAAAAGAAAGTTTGAAGAGAATATTCAGATACGTCATCAAAATAACTATCCGAAAGATGTTCTGGTGCCGCATGAAACCAACTTTGAGGATAATGTATTTGCACGACATCATAACAACTTAAAGGAGACAATCCAAGTGCAGCATCAGAACAACTCCAAAGAGGCAGATGTTCAAGTGCAATCTCGAAGCAACTTTGAATCTGGCATGAGAGCTCAAAAATGTAATATTGGATCTTTGGAGCGTACTGCTAGAAGCAATCAAGCAAGCGGTTATGATAATAGCTATCGAGTACTTGGAAAACAAGTTGATAATGGCCAAGACACTAGAGCCTTGATACCAAGGGAAAAGTTTGGTTCATTTGTCAAGCCAAATTTTCAGTCCTGTTCCACAGAAATTAATGTCCAGAGGCAGTATCAGACAAACTATCTATTGGGCATAAGACCTCCAAATTACAATTGCAGATCTTTGGAGAGTCCTTTGAAAGGCAAAGAGAGAGCTCAATCTAATAATTGCCATCCATGGATCGAACATCAAGTTGATTGTGTTCGAGATATTGGACCTGTTGCATCATCTGAAATAGAAAGAGGAGCGAGGTTGACAGTACCTATCGAGTCAAGTTTTTCTTCCAATGTTTCCAGTCAGGTAGCTTCTTCCATGTATTTGACATTACCCTCTGTGTTAAGTAGGCCCTATGGTGAGGATCCTATCCTTGGCACTTCTTCATATAGTTATATGCAACCAACAGTTGCCGGAAACAGATTAGGCACGAACTATGAGAAAGCAAACACAATGCTCAAAGTAAATGCTCATTATGGAAACTTTACAGGCGTGAGTCACCAAGTAAGAAATGGAAGCATTGCTCAAATAGGGTCCCAAAATGTGAGTTATAGTGATAGGCAAAGCATCCCTAGTTCAAGTATTGGAACTGGGTTCCCAGTTGCACTTCATCAAGGTATGGATTTTGGTGTTAGCTTTCCAAGCCAAGCAAGTATCGAATATCTTCCTCGAGATGAAAAGAACATAAGGGGTTTGAGGATGAATGAAGGAGCCAAAATGTTATCTTCCTCGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

545

Amino Acids

61.42

Weight (kDa)

7.32

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 625
AciI CCGC 3 cut(s) 86, 629, 828
AclWI GGATC 5 cut(s) 387, 799, 1091, 1256, 1269
AcoI YGGCCR 1 cut(s) 874
AcsI RAATTY 2 cut(s) 400, 931
AfaI GTAC 3 cut(s) 805, 852, 1164
AfiI CCNNNNNNNGG 3 cut(s) 19, 1043, 1271
AgsI TTSAA 9 cut(s) 57, 131, 258, 467, 575, 734, 758, 1042, 1476
AjuI GAANNNNNNNTTGG 2 cut(s) 468, 500
AluBI AGCT 5 cut(s) 773, 843, 1058, 1208, 1533
AluI AGCT 5 cut(s) 773, 843, 1058, 1208, 1533
Alw21I GWGCWC 3 cut(s) 231, 775, 1060
Alw26I GTCTC 1 cut(s) 680
Alw44I GTGCAC 1 cut(s) 227
AlwI GGATC 5 cut(s) 387, 799, 1091, 1256, 1269
Ama87I CYCGRG 2 cut(s) 1566, 1627
AoxI GGCC 2 cut(s) 874, 1247
ApaLI GTGCAC 1 cut(s) 227
ApeKI GCWGC 4 cut(s) 97, 335, 508, 700
ApoI RAATTY 2 cut(s) 400, 931
ArsI GACNNNNNNTTYG 2 cut(s) 923, 955
AseI ATTAAT 1 cut(s) 957
Asp700I GAANNNNTTC 1 cut(s) 1560
AspS9I GGNCC 3 cut(s) 1118, 1248, 1431
AsuHPI GGTGA 4 cut(s) 20, 451, 1268, 1388
AvaI CYCGRG 2 cut(s) 1566, 1627
AvaII GGWCC 2 cut(s) 1118, 1431
AxyI CCTNAGG 1 cut(s) 14
BaeGI GKGCMC 1 cut(s) 231
BalI TGGCCA 1 cut(s) 876
BamHI GGATCC 1 cut(s) 1261
BanI GGYRCC 1 cut(s) 625
BanII GRGCYC 2 cut(s) 775, 1060
BauI CACGAG 2 cut(s) 92, 175
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 3 cut(s) 231, 775, 1060
BbvI GCAGC 4 cut(s) 109, 322, 520, 712
BccI CCATC 3 cut(s) 260, 436, 1083
BcoDI GTCTC 1 cut(s) 680
BfaI CTAG 4 cut(s) 503, 810, 885, 1470
BglII AGATCT 1 cut(s) 1022
BisI GCNGC 6 cut(s) 87, 98, 336, 509, 629, 701
BlsI GCNGC 6 cut(s) 88, 99, 337, 510, 630, 702
BmcAI AGTACT 1 cut(s) 852
Bme18I GGWCC 2 cut(s) 1118, 1431
BmeT110I CYCGRG 2 cut(s) 1566, 1627
BmgT120I GGNCC 3 cut(s) 1118, 1248, 1431
BmiI GGNNCC 6 cut(s) 627, 1263, 1432, 1433, 1493, 1609
BmrI ACTGGG 2 cut(s) 1490, 1497
BmsI GCATC 3 cut(s) 712, 1136, 1473
BmuI ACTGGG 2 cut(s) 1490, 1497
BpiI GAAGAC 1 cut(s) 45
BplI GAGNNNNNCTC 4 cut(s) 169, 201, 268, 300
BsaJI CCNNGG 4 cut(s) 339, 899, 1079, 1270
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 1043, 1271
Bse1I ACTGG 5 cut(s) 111, 318, 1197, 1492, 1496
Bse21I CCTNAGG 1 cut(s) 14
Bse3DI GCAATG 1 cut(s) 1416
BseDI CCNNGG 4 cut(s) 339, 899, 1079, 1270
BseGI GGATG 6 cut(s) 349, 420, 451, 1075, 1464, 1602
BseLI CCNNNNNNNGG 3 cut(s) 19, 1043, 1271
BseMI GCAATG 1 cut(s) 1416
BseMII CTCAG 2 cut(s) 5, 183
BseNI ACTGG 5 cut(s) 111, 318, 1197, 1492, 1496
BseRI GAGGAG 1 cut(s) 1161
BseSI GKGCMC 1 cut(s) 231
BseXI GCAGC 4 cut(s) 109, 322, 520, 712
BsgI GTGCAG 2 cut(s) 527, 719
BshFI GGCC 2 cut(s) 876, 1249
BshNI GGYRCC 1 cut(s) 625
BsiHKAI GWGCWC 3 cut(s) 231, 775, 1060
BsiHKCI CYCGRG 2 cut(s) 1566, 1627
BsiSI CCGG 1 cut(s) 1311
BslFI GGGAC 1 cut(s) 1417
BslI CCNNNNNNNGG 3 cut(s) 19, 1043, 1271
BsmAI GTCTC 1 cut(s) 680
BsmFI GGGAC 1 cut(s) 1417
BsnI GGCC 2 cut(s) 876, 1249
BsoBI CYCGRG 2 cut(s) 1566, 1627
Bsp1286I GDGCHC 3 cut(s) 231, 775, 1060
Bsp143I GATC 5 cut(s) 379, 791, 1022, 1083, 1261
Bsp19I CCATGG 2 cut(s) 339, 1079
BspACI CCGC 3 cut(s) 86, 629, 828
BspANI GGCC 2 cut(s) 876, 1249
BspCNI CTCAG 2 cut(s) 6, 184
BspHI TCATGA 2 cut(s) 262, 286
BspLI GGNNCC 6 cut(s) 627, 1263, 1432, 1433, 1493, 1609
BspPI GGATC 5 cut(s) 387, 799, 1091, 1256, 1269
BspT107I GGYRCC 1 cut(s) 625
BsrDI GCAATG 1 cut(s) 1416
BsrI ACTGG 5 cut(s) 111, 318, 1197, 1492, 1496
BssECI CCNNGG 4 cut(s) 339, 899, 1079, 1270
BssMI GATC 5 cut(s) 379, 791, 1022, 1083, 1261
BssSI CACGAG 2 cut(s) 92, 175
BssT1I CCWWGG 4 cut(s) 339, 899, 1079, 1270
Bst2BI CACGAG 2 cut(s) 92, 175
Bst4CI ACNGT 2 cut(s) 1162, 1306
Bst6I CTCTTC 2 cut(s) 459, 570
BstAPI GCANNNNNTGC 1 cut(s) 427
BstC8I GCNNGC 2 cut(s) 333, 826
BstDEI CTNAG 4 cut(s) 14, 192, 383, 540
BstDSI CCRYGG 2 cut(s) 339, 1079
BstEII GGTNACC 1 cut(s) 8
BstENI CCTNNNNNAGG 1 cut(s) 1041
BstF5I GGATG 6 cut(s) 349, 420, 451, 1075, 1464, 1602
BstKTI GATC 5 cut(s) 382, 794, 1025, 1086, 1264
BstMAI GTCTC 1 cut(s) 680
BstMBI GATC 5 cut(s) 379, 791, 1022, 1083, 1261
BstMWI GCNNNNNNNGC 2 cut(s) 427, 1539
BstPI GGTNACC 1 cut(s) 8
BstSLI GKGCMC 1 cut(s) 231
BstV1I GCAGC 4 cut(s) 109, 322, 520, 712
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 4 cut(s) 379, 791, 1022, 1261
BstYI RGATCY 4 cut(s) 379, 791, 1022, 1261
Bsu36I CCTNAGG 1 cut(s) 14
BsuRI GGCC 2 cut(s) 876, 1249
BtgI CCRYGG 2 cut(s) 339, 1079
BtgZI GCGATG 1 cut(s) 60
BtsCI GGATG 6 cut(s) 349, 420, 451, 1075, 1464, 1602
Cac8I GCNNGC 2 cut(s) 333, 826
CciI TCATGA 2 cut(s) 262, 286
Cfr13I GGNCC 3 cut(s) 1118, 1248, 1431
Csp6I GTAC 3 cut(s) 804, 851, 1163
CviQI GTAC 3 cut(s) 804, 851, 1163
DdeI CTNAG 4 cut(s) 14, 192, 383, 540
DpnI GATC 5 cut(s) 381, 793, 1024, 1085, 1263
DpnII GATC 5 cut(s) 379, 791, 1022, 1083, 1261
EaeI YGGCCR 1 cut(s) 874
Eam1104I CTCTTC 2 cut(s) 459, 570
EarI CTCTTC 2 cut(s) 459, 570
Ecl136II GAGCTC 2 cut(s) 773, 1058
Eco130I CCWWGG 4 cut(s) 339, 899, 1079, 1270
Eco24I GRGCYC 2 cut(s) 775, 1060
Eco47I GGWCC 2 cut(s) 1118, 1431
Eco53kI GAGCTC 2 cut(s) 773, 1058
Eco81I CCTNAGG 1 cut(s) 14
Eco88I CYCGRG 2 cut(s) 1566, 1627
Eco91I GGTNACC 1 cut(s) 8
EcoICRI GAGCTC 2 cut(s) 773, 1058
EcoNI CCTNNNNNAGG 1 cut(s) 1041
EcoO109I RGGNCCY 2 cut(s) 1248, 1431
EcoO65I GGTNACC 1 cut(s) 8
EcoT14I CCWWGG 4 cut(s) 339, 899, 1079, 1270
EcoT38I GRGCYC 2 cut(s) 775, 1060
ErhI CCWWGG 4 cut(s) 339, 899, 1079, 1270
FalI AAGNNNNNCTT 2 cut(s) 1168, 1200
FaqI GGGAC 1 cut(s) 1417
Fnu4HI GCNGC 6 cut(s) 87, 98, 336, 509, 629, 701
FokI GGATG 6 cut(s) 356, 427, 458, 1062, 1451, 1609
FriOI GRGCYC 2 cut(s) 775, 1060
Fsp4HI GCNGC 6 cut(s) 87, 98, 336, 509, 629, 701
FspBI CTAG 4 cut(s) 503, 810, 885, 1470
GluI GCNGC 6 cut(s) 87, 98, 336, 509, 629, 701
HaeIII GGCC 2 cut(s) 876, 1249
HapII CCGG 1 cut(s) 1311
HincII GTYRAC 1 cut(s) 1158
HindII GTYRAC 1 cut(s) 1158
HpaII CCGG 1 cut(s) 1311
HphI GGTGA 4 cut(s) 20, 451, 1268, 1388
Hpy166II GTNNAC 3 cut(s) 229, 559, 1158
Hpy188I TCNGA 5 cut(s) 588, 612, 708, 978, 1135
Hpy8I GTNNAC 3 cut(s) 229, 559, 1158
HpyAV CCTTC 3 cut(s) 28, 287, 1598
HpyCH4III ACNGT 2 cut(s) 1162, 1306
HpyCH4IV ACGT 1 cut(s) 592
HpyF10VI GCNNNNNNNGC 2 cut(s) 427, 1539
HpyF3I CTNAG 4 cut(s) 14, 192, 383, 540
HpySE526I ACGT 1 cut(s) 592
KflI GGGWCCC 1 cut(s) 1431
Kzo9I GATC 5 cut(s) 379, 791, 1022, 1083, 1261
LmnI GCTCC 3 cut(s) 799, 1148, 1607
Lsp1109I GCAGC 4 cut(s) 109, 322, 520, 712
LweI GCATC 3 cut(s) 712, 1136, 1473
MaeI CTAG 4 cut(s) 503, 810, 885, 1470
MaeII ACGT 1 cut(s) 592
MaeIII GTNAC 4 cut(s) 8, 172, 388, 1394
MalI GATC 5 cut(s) 381, 793, 1024, 1085, 1263
MboI GATC 5 cut(s) 379, 791, 1022, 1083, 1261
MfeI CAATTG 1 cut(s) 1015
MflI RGATCY 4 cut(s) 379, 791, 1022, 1261
MhlI GDGCHC 3 cut(s) 231, 775, 1060
MlsI TGGCCA 1 cut(s) 876
MluNI TGGCCA 1 cut(s) 876
MlyI GAGTC 6 cut(s) 59, 168, 374, 1042, 1181, 1402
Mox20I TGGCCA 1 cut(s) 876
MroXI GAANNNNTTC 1 cut(s) 1560
MscI TGGCCA 1 cut(s) 876
MseI TTAA 5 cut(s) 198, 351, 680, 957, 1241
Msp20I TGGCCA 1 cut(s) 876
MspI CCGG 1 cut(s) 1311
MunI CAATTG 1 cut(s) 1015
MwoI GCNNNNNNNGC 2 cut(s) 427, 1539
NcoI CCATGG 2 cut(s) 339, 1079
NdeII GATC 5 cut(s) 379, 791, 1022, 1083, 1261
NlaIV GGNNCC 6 cut(s) 627, 1263, 1432, 1433, 1493, 1609
NmuCI GTSAC 4 cut(s) 8, 172, 388, 1394
PaeR7I CTCGAG 2 cut(s) 1566, 1627
PagI TCATGA 2 cut(s) 262, 286
PdmI GAANNNNTTC 1 cut(s) 1560
PfeI GAWTC 4 cut(s) 65, 281, 357, 758
PkrI GCNGC 6 cut(s) 88, 99, 337, 510, 630, 702
PleI GAGTC 6 cut(s) 58, 167, 373, 1041, 1180, 1401
PpsI GAGTC 6 cut(s) 58, 167, 373, 1041, 1180, 1401
PpuMI RGGWCCY 1 cut(s) 1431
PshBI ATTAAT 1 cut(s) 957
Psp124BI GAGCTC 2 cut(s) 775, 1060
Psp5II RGGWCCY 1 cut(s) 1431
PspEI GGTNACC 1 cut(s) 8
PspN4I GGNNCC 6 cut(s) 627, 1263, 1432, 1433, 1493, 1609
PspPI GGNCC 3 cut(s) 1118, 1248, 1431
PspPPI RGGWCCY 1 cut(s) 1431
PsuI RGATCY 4 cut(s) 379, 791, 1022, 1261
RsaI GTAC 3 cut(s) 805, 852, 1164
RsaNI GTAC 3 cut(s) 804, 851, 1163
SacI GAGCTC 2 cut(s) 775, 1060
SaqAI TTAA 5 cut(s) 198, 351, 680, 957, 1241
SatI GCNGC 6 cut(s) 87, 98, 336, 509, 629, 701
Sau3AI GATC 5 cut(s) 379, 791, 1022, 1083, 1261
Sau96I GGNCC 3 cut(s) 1118, 1248, 1431
ScaI AGTACT 1 cut(s) 852
SchI GAGTC 6 cut(s) 59, 168, 374, 1042, 1181, 1402
SduI GDGCHC 3 cut(s) 231, 775, 1060
SfaNI GCATC 3 cut(s) 712, 1136, 1473
Sfr274I CTCGAG 2 cut(s) 1566, 1627
SinI GGWCC 2 cut(s) 1118, 1431
SlaI CTCGAG 2 cut(s) 1566, 1627
SmlI CTYRAG 2 cut(s) 1566, 1627
SmoI CTYRAG 2 cut(s) 1566, 1627
SsiI CCGC 3 cut(s) 86, 629, 828
SspI AATATT 2 cut(s) 583, 787
SspMI CTAG 4 cut(s) 503, 810, 885, 1470
SstI GAGCTC 2 cut(s) 775, 1060
StyI CCWWGG 4 cut(s) 339, 899, 1079, 1270
TaaI ACNGT 2 cut(s) 1162, 1306
TaiI ACGT 1 cut(s) 595
TaqI TCGA 8 cut(s) 745, 847, 1086, 1108, 1170, 1554, 1567, 1628
TatI WGTACW 1 cut(s) 850
TauI GCSGC 2 cut(s) 89, 631
TfiI GAWTC 4 cut(s) 65, 281, 357, 758
Tru1I TTAA 5 cut(s) 198, 351, 680, 957, 1241
Tru9I TTAA 5 cut(s) 198, 351, 680, 957, 1241
TseFI GTSAC 4 cut(s) 8, 172, 388, 1394
TseI GCWGC 4 cut(s) 97, 335, 508, 700
Tsp45I GTSAC 4 cut(s) 8, 172, 388, 1394
TspGWI ACGGA 1 cut(s) 484
VneI GTGCAC 1 cut(s) 227
VpaK11BI GGWCC 2 cut(s) 1118, 1431
VspI ATTAAT 1 cut(s) 957
XagI CCTNNNNNAGG 1 cut(s) 1041
XapI RAATTY 2 cut(s) 400, 931
XcmI CCANNNNNNNNNTGG 1 cut(s) 1406
XhoI CTCGAG 2 cut(s) 1566, 1627
XmnI GAANNNNTTC 1 cut(s) 1560
XspI CTAG 4 cut(s) 503, 810, 885, 1470
ZrmI AGTACT 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.