pycom06g08950

2S seed storage protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0017371
Physical Location & Seq
Forward (+)
14196691 .. 14198624
1934 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g08950.1

Sequence Viewer

Length: 1704 bp
ATGAATGGGCATAGCAACACTTATTGTATTTGTAGAACCTTGTTGAATCAATGCTATGTTTTAAGTGGCCTCTTTAGAAGTTCCCTTAATATTAAAGATACAAAAAGTTTGAAGTTTTTTTTTTCATTGAAATTAATAGCGAAGCAGGAGGTGAAGAAAGCTGTGAGAGAAGCTAAGCTAGCGACTTATGACAATATGTATAAGCGACTAGATACCAAAGAAGGAGAGTTGGATATCTATAAACTAGCTAGAGCAAAGGAAAAGAAGACAAGGGACCCAAATCAAGTGAGGTGCATCAAGGATGAGGATGGAAAGGTTCTTGCTACAGAGAATGCGGTCAAAGACAGATGGAGAGGTTATTTTCATAATTTTTTCAATGAAGGACATGAAAGGAGTACTTCTTTGGGGGAGTTGAGTAACTCAGAAGAGTTGGTTGTAGCTTTGAAAAAGACGAAGCATAGAAAAACAGTGGGCCCAGATGATATACCGATCGAAGTGTGGAAAGTCTTGGAAGAGACGGGTATATCATGGCTCACAAACCTTTTCAATAGGATTTTGAAAACGAAGAAGATGCCAAATGAGTGGCGAAAGAGCACTTTGGTGCCTATCTACAAAAATAAGGGCGACGTACAAAATTGCATGAACTATAGGGGTATTAAGCTAATGAGTCATACAATGAAGCTCTGGGAGAGAGTCATTGAGCATAGATTGAGGCAAGAGACACGGGTTTTGGACAACCAATTCGGGTTCATGCCAAGGCGCTCAACCATGGAGGCAATCTATCTCTTACGAAGATTGATGGAAAGATATAGAGATAGGAAAAAGCATTTACACATGGCCTTTATAGATTTGGAAAAAAAGAAAGGAGTACAGGTAGCATATATCCAAGCTATAAAGGATATGTATGATGGAGCAAAGACTGCCGTAAGAACTCATGAAGGACAAACCGAAAGCTTCCCCATAACTGTAAGGTTACATCAAGGCTCATCCTTAAATGATATCCTTTGGTGTATGCTTTTCGCAGACGATATAGTGTTGATAGATGAAACTCAGGAAGGGGTAAATGCGAAGCTTAACCTTTGGAGAAAAGTGTTGGAATCTAAAGGTCTTTGCCTAAGCCAATCAAAGACAGAATATATGGGGTACAAGTTTAGTGCAAATGGAGGCCAAAATGAGTTAGGGGTGAAGATCGATCGGGATTCCCCGTGTCAAGAAGTGCCAAAGATCGACCACTTTCGCTACCTCGGATCTATCTTGCAAAAGAACGGAAAATTAGATAGAGACCTCAACCATAGAATACAAGCTGGATGGATGAAGTGGAAGAGTGCATCCGGCGTGTTGTGTGACCGCCGTATGCCACTGAAGCTTAAGGGAAAACTTTATAGGACGACAATAAGGCCGGTGATGCTGTATGGCACAAAATGTTCGGCGGTGAAGCATCAAAACGTACATAAAATAGGTGTAGCGGAGATGAGGATGCCTTTTTGGATGTGTGGGCGCACGAGAAAGGATAAGATTAGAAATGAGGATATCCGAGGTAAAGTAGGAGTAGCCGAAATTGAAGGAAAGATGAGAGAAAATCGGTTATGGCGACTAAGGACAGAGGTCTATGGCCGAAGGGGTAAAGGAAGACCTAGGAAAACTTTGGAAGAGACTCTAAGAAAAGACTTAGAGTACTTGGATCTAACGGAGGACATGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Tryp_alpha_amyl

Protein Analysis

568

Amino Acids

66.42

Weight (kDa)

9.76

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 208 - 286 3.5e-06 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 601
AciI CCGC 4 cut(s) 335, 1348, 1430, 1466
AclWI GGATC 2 cut(s) 1255, 1689
AcoI YGGCCR 1 cut(s) 1612
AcuI CTGAAG 1 cut(s) 1382
AfaI GTAC 6 cut(s) 397, 630, 870, 1145, 1449, 1676
AflII CTTAAG 1 cut(s) 1367
AgsI TTSAA 8 cut(s) 46, 112, 130, 376, 445, 547, 559, 1562
AleI CACNNNNGTG 1 cut(s) 599
Alw21I GWGCWC 1 cut(s) 596
Alw26I GTCTC 4 cut(s) 509, 713, 1275, 1646
AlwI GGATC 2 cut(s) 1255, 1689
AoxI GGCC 6 cut(s) 67, 472, 837, 1165, 1397, 1612
ApaI GGGCCC 1 cut(s) 476
ArsI GACNNNNNNTTYG 4 cut(s) 712, 725, 744, 757
AseI ATTAAT 1 cut(s) 134
AspA2I CCTAGG 1 cut(s) 1634
AspLEI GCGC 2 cut(s) 762, 1500
AspS9I GGNCC 3 cut(s) 274, 472, 473
AsuHPI GGTGA 4 cut(s) 163, 1195, 1414, 1444
AsuNHI GCTAGC 1 cut(s) 178
AvaII GGWCC 1 cut(s) 274
AvrII CCTAGG 1 cut(s) 1634
BaeGI GKGCMC 1 cut(s) 476
BanI GGYRCC 1 cut(s) 601
BanII GRGCYC 1 cut(s) 476
BauI CACGAG 1 cut(s) 1501
BbsI GAAGAC 2 cut(s) 272, 1636
Bbv12I GWGCWC 1 cut(s) 596
BccI CCATC 5 cut(s) 302, 342, 793, 902, 1302
BceAI ACGGC 2 cut(s) 908, 1335
BcgI CGANNNNNNTGC 2 cut(s) 553, 587
BcoDI GTCTC 4 cut(s) 509, 713, 1275, 1646
BfaI CTAG 5 cut(s) 179, 209, 245, 249, 1635
BfmI CTRYAG 2 cut(s) 324, 646
BfoI RGCGCY 1 cut(s) 763
BfrI CTTAAG 1 cut(s) 1367
BlnI CCTAGG 1 cut(s) 1634
BlpI GCTNAGC 1 cut(s) 174
BmcAI AGTACT 2 cut(s) 397, 1676
Bme18I GGWCC 1 cut(s) 274
BmgT120I GGNCC 3 cut(s) 274, 472, 473
BmiI GGNNCC 4 cut(s) 275, 276, 474, 603
BmsI GCATC 6 cut(s) 303, 561, 1337, 1395, 1447, 1467
BmtI GCTAGC 1 cut(s) 182
BoxI GACNNNNGTC 1 cut(s) 1604
BpiI GAAGAC 2 cut(s) 272, 1636
Bpu10I CCTNAGC 1 cut(s) 1115
Bpu1102I GCTNAGC 1 cut(s) 174
Bsa29I ATCGAT 1 cut(s) 1191
BsaI GGTCTC 1 cut(s) 1275
BsaJI CCNNGG 5 cut(s) 755, 768, 1243, 1534, 1634
BsaXI ACNNNNNCTCC 2 cut(s) 858, 888
Bse118I RCCGGY 1 cut(s) 1399
BseCI ATCGAT 1 cut(s) 1191
BseDI CCNNGG 5 cut(s) 755, 768, 1243, 1534, 1634
BseGI GGATG 8 cut(s) 307, 313, 986, 1313, 1317, 1328, 1482, 1494
BseMII CTCAG 2 cut(s) 435, 1064
BseSI GKGCMC 1 cut(s) 476
Bsh1285I CGRYCG 2 cut(s) 492, 1195
BshFI GGCC 6 cut(s) 69, 474, 839, 1167, 1399, 1614
BshNI GGYRCC 1 cut(s) 601
BshVI ATCGAT 1 cut(s) 1191
BsiEI CGRYCG 2 cut(s) 492, 1195
BsiHKAI GWGCWC 1 cut(s) 596
BsiSI CCGG 2 cut(s) 1332, 1400
BslFI GGGAC 1 cut(s) 287
BsmAI GTCTC 4 cut(s) 509, 713, 1275, 1646
BsmBI CGTCTC 1 cut(s) 509
BsmFI GGGAC 1 cut(s) 287
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 6 cut(s) 69, 474, 839, 1167, 1399, 1614
Bso31I GGTCTC 1 cut(s) 1275
Bsp120I GGGCCC 1 cut(s) 472
Bsp1286I GDGCHC 2 cut(s) 476, 596
Bsp143I GATC 6 cut(s) 489, 1188, 1192, 1224, 1247, 1681
Bsp1720I GCTNAGC 1 cut(s) 174
Bsp19I CCATGG 1 cut(s) 768
BspACI CCGC 4 cut(s) 335, 1348, 1430, 1466
BspANI GGCC 6 cut(s) 69, 474, 839, 1167, 1399, 1614
BspCNI CTCAG 2 cut(s) 434, 1063
BspDI ATCGAT 1 cut(s) 1191
BspHI TCATGA 1 cut(s) 934
BspLI GGNNCC 4 cut(s) 275, 276, 474, 603
BspOI GCTAGC 1 cut(s) 182
BspPI GGATC 2 cut(s) 1255, 1689
BspT107I GGYRCC 1 cut(s) 601
BspTI CTTAAG 1 cut(s) 1367
BspTNI GGTCTC 1 cut(s) 1275
BsrFI RCCGGY 1 cut(s) 1399
BssAI RCCGGY 1 cut(s) 1399
BssECI CCNNGG 5 cut(s) 755, 768, 1243, 1534, 1634
BssMI GATC 6 cut(s) 489, 1188, 1192, 1224, 1247, 1681
BssSI CACGAG 1 cut(s) 1501
BssT1I CCWWGG 3 cut(s) 755, 768, 1634
Bst2BI CACGAG 1 cut(s) 1501
Bst4CI ACNGT 2 cut(s) 469, 967
Bst6I CTCTTC 4 cut(s) 420, 507, 1316, 1644
BstAFI CTTAAG 1 cut(s) 1367
BstAPI GCANNNNNTGC 1 cut(s) 920
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 7 cut(s) 174, 421, 1050, 1115, 1595, 1658, 1669
BstDSI CCRYGG 1 cut(s) 768
BstF5I GGATG 8 cut(s) 307, 313, 986, 1313, 1317, 1328, 1482, 1494
BstH2I RGCGCY 1 cut(s) 763
BstHHI GCGC 2 cut(s) 762, 1500
BstKTI GATC 6 cut(s) 492, 1191, 1195, 1227, 1250, 1684
BstMAI GTCTC 4 cut(s) 509, 713, 1275, 1646
BstMBI GATC 6 cut(s) 489, 1188, 1192, 1224, 1247, 1681
BstMCI CGRYCG 2 cut(s) 492, 1195
BstMWI GCNNNNNNNGC 4 cut(s) 179, 920, 1363, 1405
BstPAI GACNNNNGTC 1 cut(s) 1604
BstSFI CTRYAG 2 cut(s) 324, 646
BstSLI GKGCMC 1 cut(s) 476
BstV2I GAAGAC 2 cut(s) 272, 1636
BstX2I RGATCY 2 cut(s) 1247, 1681
BstXI CCANNNNNNTGG 1 cut(s) 582
BstYI RGATCY 2 cut(s) 1247, 1681
Bsu15I ATCGAT 1 cut(s) 1191
BsuRI GGCC 6 cut(s) 69, 474, 839, 1167, 1399, 1614
BsuTUI ATCGAT 1 cut(s) 1191
BtgI CCRYGG 1 cut(s) 768
BtsCI GGATG 8 cut(s) 307, 313, 986, 1313, 1317, 1328, 1482, 1494
BtsIMutI CAGTG 2 cut(s) 474, 1358
Cac8I GCNNGC 1 cut(s) 180
CciI TCATGA 1 cut(s) 934
CfoI GCGC 2 cut(s) 762, 1500
Cfr10I RCCGGY 1 cut(s) 1399
Cfr13I GGNCC 3 cut(s) 274, 472, 473
ClaI ATCGAT 1 cut(s) 1191
Csp6I GTAC 6 cut(s) 396, 629, 869, 1144, 1448, 1675
CviAII CATG 8 cut(s) 386, 528, 640, 751, 769, 835, 935, 1696
CviQI GTAC 6 cut(s) 396, 629, 869, 1144, 1448, 1675
DdeI CTNAG 7 cut(s) 174, 421, 1050, 1115, 1595, 1658, 1669
DpnI GATC 6 cut(s) 491, 1190, 1194, 1226, 1249, 1683
DpnII GATC 6 cut(s) 489, 1188, 1192, 1224, 1247, 1681
EaeI YGGCCR 1 cut(s) 1612
Eam1104I CTCTTC 4 cut(s) 420, 507, 1316, 1644
EarI CTCTTC 4 cut(s) 420, 507, 1316, 1644
Eco130I CCWWGG 3 cut(s) 755, 768, 1634
Eco24I GRGCYC 1 cut(s) 476
Eco31I GGTCTC 1 cut(s) 1275
Eco32I GATATC 3 cut(s) 235, 1000, 1531
Eco47I GGWCC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 1382
EcoO109I RGGNCCY 1 cut(s) 274
EcoRV GATATC 3 cut(s) 235, 1000, 1531
EcoT14I CCWWGG 3 cut(s) 755, 768, 1634
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 3 cut(s) 755, 768, 1634
Esp3I CGTCTC 1 cut(s) 509
FaeI CATG 8 cut(s) 389, 531, 643, 754, 772, 838, 938, 1699
FalI AAGNNNNNCTT 2 cut(s) 382, 414
FaqI GGGAC 1 cut(s) 287
FatI CATG 8 cut(s) 385, 527, 639, 750, 768, 834, 934, 1695
FokI GGATG 8 cut(s) 314, 320, 973, 1315, 1320, 1324, 1489, 1501
FriOI GRGCYC 1 cut(s) 476
FspBI CTAG 5 cut(s) 179, 209, 245, 249, 1635
GlaI GCGC 2 cut(s) 761, 1499
HaeII RGCGCY 1 cut(s) 763
HaeIII GGCC 6 cut(s) 69, 474, 839, 1167, 1399, 1614
HapII CCGG 2 cut(s) 1332, 1400
HhaI GCGC 2 cut(s) 762, 1500
Hin1II CATG 8 cut(s) 389, 531, 643, 754, 772, 838, 938, 1699
Hin6I GCGC 2 cut(s) 760, 1498
HinP1I GCGC 2 cut(s) 760, 1498
HindIII AAGCTT 3 cut(s) 952, 1070, 1364
HinfI GANTC 6 cut(s) 46, 667, 693, 1097, 1199, 1654
HpaII CCGG 2 cut(s) 1332, 1400
HphI GGTGA 4 cut(s) 163, 1195, 1414, 1444
Hpy188I TCNGA 3 cut(s) 424, 1247, 1535
Hpy188III TCNNGA 4 cut(s) 935, 1052, 1196, 1211
Hpy99I CGWCG 1 cut(s) 629
HpyAV CCTTC 6 cut(s) 215, 374, 932, 1049, 1556, 1611
HpyCH4III ACNGT 2 cut(s) 469, 967
HpyCH4IV ACGT 2 cut(s) 627, 1446
HpyCH4V TGCA 5 cut(s) 294, 639, 1157, 1258, 1328
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 920, 1363, 1405
HpyF3I CTNAG 7 cut(s) 174, 421, 1050, 1115, 1595, 1658, 1669
HpySE526I ACGT 2 cut(s) 627, 1446
Hsp92II CATG 8 cut(s) 389, 531, 643, 754, 772, 838, 938, 1699
HspAI GCGC 2 cut(s) 760, 1498
KflI GGGWCCC 1 cut(s) 274
Kzo9I GATC 6 cut(s) 489, 1188, 1192, 1224, 1247, 1681
LmnI GCTCC 1 cut(s) 911
LpnPI CCDG 8 cut(s) 131, 489, 670, 857, 1037, 1290, 1345, 1413
LweI GCATC 6 cut(s) 303, 561, 1337, 1395, 1447, 1467
MaeI CTAG 5 cut(s) 179, 209, 245, 249, 1635
MaeII ACGT 2 cut(s) 627, 1446
MaeIII GTNAC 3 cut(s) 416, 972, 1343
MalI GATC 6 cut(s) 491, 1190, 1194, 1226, 1249, 1683
MboI GATC 6 cut(s) 489, 1188, 1192, 1224, 1247, 1681
MflI RGATCY 2 cut(s) 1247, 1681
MhlI GDGCHC 2 cut(s) 476, 596
MluCI AATT 6 cut(s) 131, 367, 634, 740, 1271, 1557
MlyI GAGTC 3 cut(s) 676, 702, 1648
MmeI TCCRAC 2 cut(s) 210, 1074
MseI TTAA 8 cut(s) 62, 87, 93, 134, 657, 992, 1074, 1368
MslI CAYNNNNRTG 1 cut(s) 599
MspCI CTTAAG 1 cut(s) 1367
MspI CCGG 2 cut(s) 1332, 1400
Mva1269I GAATGC 1 cut(s) 337
MwoI GCNNNNNNNGC 4 cut(s) 179, 920, 1363, 1405
NcoI CCATGG 1 cut(s) 768
NdeII GATC 6 cut(s) 489, 1188, 1192, 1224, 1247, 1681
NheI GCTAGC 1 cut(s) 178
NlaIII CATG 8 cut(s) 389, 531, 643, 754, 772, 838, 938, 1699
NlaIV GGNNCC 4 cut(s) 275, 276, 474, 603
NmuCI GTSAC 1 cut(s) 1343
OliI CACNNNNGTG 1 cut(s) 599
PagI TCATGA 1 cut(s) 934
PcsI WCGNNNNNNNCGW 1 cut(s) 1588
PctI GAATGC 1 cut(s) 337
PfeI GAWTC 3 cut(s) 46, 1097, 1199
Ple19I CGATCG 2 cut(s) 492, 1195
PleI GAGTC 3 cut(s) 675, 701, 1648
PpsI GAGTC 3 cut(s) 675, 701, 1648
PpuMI RGGWCCY 1 cut(s) 274
PshAI GACNNNNGTC 1 cut(s) 1604
PshBI ATTAAT 1 cut(s) 134
Psp5II RGGWCCY 1 cut(s) 274
PspN4I GGNNCC 4 cut(s) 275, 276, 474, 603
PspOMI GGGCCC 1 cut(s) 472
PspPI GGNCC 3 cut(s) 274, 472, 473
PspPPI RGGWCCY 1 cut(s) 274
PsuI RGATCY 2 cut(s) 1247, 1681
PvuI CGATCG 2 cut(s) 492, 1195
RsaI GTAC 6 cut(s) 397, 630, 870, 1145, 1449, 1676
RsaNI GTAC 6 cut(s) 396, 629, 869, 1144, 1448, 1675
RseI CAYNNNNRTG 1 cut(s) 599
SaqAI TTAA 8 cut(s) 62, 87, 93, 134, 657, 992, 1074, 1368
Sau3AI GATC 6 cut(s) 489, 1188, 1192, 1224, 1247, 1681
Sau96I GGNCC 3 cut(s) 274, 472, 473
ScaI AGTACT 2 cut(s) 397, 1676
SchI GAGTC 3 cut(s) 676, 702, 1648
SduI GDGCHC 2 cut(s) 476, 596
SfaNI GCATC 6 cut(s) 303, 561, 1337, 1395, 1447, 1467
SfcI CTRYAG 2 cut(s) 324, 646
SinI GGWCC 1 cut(s) 274
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 1 cut(s) 1367
SmoI CTYRAG 1 cut(s) 1367
Sse9I AATT 6 cut(s) 131, 367, 634, 740, 1271, 1557
SsiI CCGC 4 cut(s) 335, 1348, 1430, 1466
SspI AATATT 1 cut(s) 91
SspMI CTAG 5 cut(s) 179, 209, 245, 249, 1635
StyI CCWWGG 3 cut(s) 755, 768, 1634
TaaI ACNGT 2 cut(s) 469, 967
TaiI ACGT 2 cut(s) 630, 1449
TaqI TCGA 3 cut(s) 492, 1191, 1227
TasI AATT 6 cut(s) 131, 367, 634, 740, 1271, 1557
TatI WGTACW 3 cut(s) 395, 868, 1674
TfiI GAWTC 3 cut(s) 46, 1097, 1199
Tru1I TTAA 8 cut(s) 62, 87, 93, 134, 657, 992, 1074, 1368
Tru9I TTAA 8 cut(s) 62, 87, 93, 134, 657, 992, 1074, 1368
TscAI CASTG 2 cut(s) 474, 1365
TseFI GTSAC 1 cut(s) 1343
Tsp45I GTSAC 1 cut(s) 1343
TspGWI ACGGA 2 cut(s) 1281, 1703
TspRI CASTG 2 cut(s) 474, 1365
Vha464I CTTAAG 1 cut(s) 1367
VpaK11BI GGWCC 1 cut(s) 274
VspI ATTAAT 1 cut(s) 134
XmaJI CCTAGG 1 cut(s) 1634
XspI CTAG 5 cut(s) 179, 209, 245, 249, 1635
ZrmI AGTACT 2 cut(s) 397, 1676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.