pycom12g15660

2S seed storage protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0017371
Physical Location & Seq
Forward (+)
18120680 .. 18120919
240 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g15660.1

Sequence Viewer

Length: 240 bp
ATGTATGATGAAGCAAAGACTGCCGTAAGAACTCATGAAGGACAAACCGAAAGCTTCCCCATAATTGTAGGATTACATCAAGGCTTATCCTTAAGTCCTTACCTTTTTGCATTGGTAATGGATGAGTTAACGGGACATATTCAAGATGATATTCCTTGGTGTATGCTTTTCGCAGATGATATAGTGTTGATAGATGAAACTCAGGAAGGGGTAAATGCGAAGCTTAACCTTTGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Tryp_alpha_amyl

Protein Analysis

80

Amino Acids

8.96

Weight (kDa)

4.32

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 9 - 74 2.9e-09 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AflII CTTAAG 1 cut(s) 91
AgsI TTSAA 1 cut(s) 143
AluBI AGCT 2 cut(s) 54, 223
AluI AGCT 2 cut(s) 54, 223
BceAI ACGGC 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 91
BsaJI CCNNGG 1 cut(s) 155
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 1 cut(s) 127
BseMII CTCAG 1 cut(s) 215
BslFI GGGAC 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 147
BspCNI CTCAG 1 cut(s) 214
BspHI TCATGA 1 cut(s) 34
BspTI CTTAAG 1 cut(s) 91
BssECI CCNNGG 1 cut(s) 155
BssT1I CCWWGG 1 cut(s) 155
BstAFI CTTAAG 1 cut(s) 91
BstAPI GCANNNNNTGC 1 cut(s) 20
BstDEI CTNAG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 127
BstMWI GCNNNNNNNGC 1 cut(s) 20
BtsCI GGATG 1 cut(s) 127
CciI TCATGA 1 cut(s) 34
CviAII CATG 1 cut(s) 35
CviJI RGCY 3 cut(s) 54, 84, 223
CviKI_1 RGCY 3 cut(s) 54, 84, 223
DdeI CTNAG 1 cut(s) 201
Eco130I CCWWGG 1 cut(s) 155
EcoT14I CCWWGG 1 cut(s) 155
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 1 cut(s) 38
FaiI YATR 6 cut(s) 6, 36, 62, 138, 164, 182
FaqI GGGAC 1 cut(s) 147
FatI CATG 1 cut(s) 34
FokI GGATG 1 cut(s) 134
Hin1II CATG 1 cut(s) 38
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HindIII AAGCTT 2 cut(s) 52, 221
HpaI GTTAAC 1 cut(s) 129
Hpy166II GTNNAC 1 cut(s) 129
Hpy188III TCNNGA 3 cut(s) 35, 143, 203
Hpy8I GTNNAC 1 cut(s) 129
HpyAV CCTTC 2 cut(s) 32, 200
HpyCH4V TGCA 1 cut(s) 110
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 1 cut(s) 201
Hsp92II CATG 1 cut(s) 38
KspAI GTTAAC 1 cut(s) 129
LpnPI CCDG 1 cut(s) 188
MluCI AATT 1 cut(s) 63
MseI TTAA 3 cut(s) 92, 128, 225
MspCI CTTAAG 1 cut(s) 91
MwoI GCNNNNNNNGC 1 cut(s) 20
NlaIII CATG 1 cut(s) 38
PagI TCATGA 1 cut(s) 34
SaqAI TTAA 3 cut(s) 92, 128, 225
SetI ASST 4 cut(s) 56, 105, 225, 231
SgeI CNNG 6 cut(s) 47, 92, 144, 155, 168, 215
SmlI CTYRAG 1 cut(s) 91
SmoI CTYRAG 1 cut(s) 91
Sse9I AATT 1 cut(s) 63
StyI CCWWGG 1 cut(s) 155
TasI AATT 1 cut(s) 63
Tru1I TTAA 3 cut(s) 92, 128, 225
Tru9I TTAA 3 cut(s) 92, 128, 225
TspDTI ATGAA 3 cut(s) 24, 51, 210
Vha464I CTTAAG 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.