pycom13g26750

2S seed storage protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0017371
Physical Location & Seq
Forward (+)
23270952 .. 23271812
861 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g26750.4

Sequence Viewer

Length: 714 bp
ATGGACTGTGGTAAAGACTGTGGTAATGATCTATGTGCTAGAGAGTTTGAGATGAATTCGAACAATTCATTTGTGGAGAAGAGCGAGAATATTTCTAACCTAATTTCTATTTTCAAATATTTGATTGCATGGCATGCTCGTTTGATTGTTTTGCTATATATATATTCCAACACTTCTCGCCAAAGGTTAAGCTTTGCATTTACTCTCTCCCATGTTTTATCTATCAACACTATATCATTTGTGAAAAGCATAACCAAAGGATATCATCTTGAGTATGTCTCGTTAACTTGTCCACTACCAAGGCAAAAAGGTAAGGACTTAAAACATAGTATATGGAGTGCAAGTTCAATCAGGAAGTACCAAAGAGTGAAGCGTTATCTTGGATCTATCTTGCAAAAGAATGGAGAATTAGATGGAGATCTCAACCATAGAATACAAGCTGGATGGATGAGTGCATTAGATGTGTTATGTGATCATTGTATGCTACTAAAGTTCAAGGGAAAATTTTATAGGATGGCAAATAAGACCATCAATGCTTTATGGCACAAAATGTTGGGCGGTCAAAAATCAACACGTGCAAAAAATGAGTGTAGCAGAGCTTCGTTGGATTTGTGGGCACACATAAAGGATATGATGACGAACGAGGATATCCGAGATAAAGTAGGAGGGCCGCAATTGAAGATAAGATGGAAAACATCGGTTAAGGTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Tryp_alpha_amyl

Protein Analysis

238

Amino Acids

27.28

Weight (kDa)

9.69

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 558, 671
AclWI GGATC 1 cut(s) 391
AcsI RAATTY 2 cut(s) 55, 503
AcvI CACGTG 1 cut(s) 575
AfaI GTAC 1 cut(s) 359
AflIII ACRYGT 1 cut(s) 572
AgsI TTSAA 4 cut(s) 115, 348, 496, 679
AloI GAACNNNNNNTCC 2 cut(s) 328, 360
AluBI AGCT 3 cut(s) 192, 440, 599
AluI AGCT 3 cut(s) 192, 440, 599
Alw26I GTCTC 1 cut(s) 283
AlwI GGATC 1 cut(s) 391
AoxI GGCC 1 cut(s) 668
ApoI RAATTY 2 cut(s) 55, 503
AspS9I GGNCC 1 cut(s) 668
AsuII TTCGAA 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 619
BarI GAAGNNNNNNTAC 2 cut(s) 583, 615
BbrPI CACGTG 1 cut(s) 575
BccI CCATC 5 cut(s) 407, 438, 508, 536, 681
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 283
BfaI CTAG 1 cut(s) 39
BglII AGATCT 1 cut(s) 418
BisI GCNGC 1 cut(s) 671
BlsI GCNGC 1 cut(s) 672
BmgT120I GGNCC 1 cut(s) 668
BplI GAGNNNNNCTC 2 cut(s) 263, 295
Bpu14I TTCGAA 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 290
BsaAI YACGTR 1 cut(s) 575
BsaBI GATNNNNATC 1 cut(s) 417
BsaJI CCNNGG 1 cut(s) 299
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bse8I GATNNNNATC 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 299
BseGI GGATG 3 cut(s) 449, 453, 519
BseJI GATNNNNATC 1 cut(s) 417
BseSI GKGCMC 1 cut(s) 619
BshFI GGCC 1 cut(s) 670
BsmAI GTCTC 1 cut(s) 283
BsnI GGCC 1 cut(s) 670
Bsp119I TTCGAA 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 619
Bsp143I GATC 4 cut(s) 28, 383, 418, 472
BspACI CCGC 2 cut(s) 558, 671
BspANI GGCC 1 cut(s) 670
BspPI GGATC 1 cut(s) 391
BspQI GCTCTTC 1 cut(s) 74
BspT104I TTCGAA 1 cut(s) 59
BssECI CCNNGG 1 cut(s) 299
BssMI GATC 4 cut(s) 28, 383, 418, 472
BssT1I CCWWGG 1 cut(s) 299
Bst4CI ACNGT 2 cut(s) 8, 20
Bst6I CTCTTC 1 cut(s) 74
BstAPI GCANNNNNTGC 1 cut(s) 134
BstBAI YACGTR 1 cut(s) 575
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 1 cut(s) 135
BstF5I GGATG 3 cut(s) 449, 453, 519
BstKTI GATC 4 cut(s) 31, 386, 421, 475
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 4 cut(s) 28, 383, 418, 472
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNSI RCATGY 1 cut(s) 137
BstSLI GKGCMC 1 cut(s) 619
BstX2I RGATCY 2 cut(s) 383, 418
BstYI RGATCY 2 cut(s) 383, 418
BsuRI GGCC 1 cut(s) 670
BtsCI GGATG 3 cut(s) 449, 453, 519
Cac8I GCNNGC 1 cut(s) 135
Cfr13I GGNCC 1 cut(s) 668
Csp6I GTAC 1 cut(s) 358
CviAII CATG 3 cut(s) 129, 134, 212
CviJI RGCY 4 cut(s) 192, 440, 599, 670
CviKI_1 RGCY 4 cut(s) 192, 440, 599, 670
CviQI GTAC 1 cut(s) 358
DpnI GATC 4 cut(s) 30, 385, 420, 474
DpnII GATC 4 cut(s) 28, 383, 418, 472
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 299
Eco32I GATATC 2 cut(s) 263, 649
Eco72I CACGTG 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 55
EcoRV GATATC 2 cut(s) 263, 649
EcoT14I CCWWGG 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 299
FaeI CATG 3 cut(s) 132, 137, 215
FatI CATG 3 cut(s) 128, 133, 211
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 1 cut(s) 671
FokI GGATG 3 cut(s) 456, 460, 526
Fsp4HI GCNGC 1 cut(s) 671
FspBI CTAG 1 cut(s) 39
GluI GCNGC 1 cut(s) 671
HaeIII GGCC 1 cut(s) 670
Hin1II CATG 3 cut(s) 132, 137, 215
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HindIII AAGCTT 1 cut(s) 190
HpaI GTTAAC 1 cut(s) 285
Hpy166II GTNNAC 2 cut(s) 285, 293
Hpy188I TCNGA 1 cut(s) 653
Hpy188III TCNNGA 2 cut(s) 269, 352
Hpy8I GTNNAC 2 cut(s) 285, 293
HpyCH4III ACNGT 2 cut(s) 8, 20
HpyCH4IV ACGT 1 cut(s) 574
HpyCH4V TGCA 6 cut(s) 128, 197, 341, 394, 455, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpySE526I ACGT 1 cut(s) 574
Hsp92II CATG 3 cut(s) 132, 137, 215
Ksp22I TGATCA 1 cut(s) 472
KspAI GTTAAC 1 cut(s) 285
Kzo9I GATC 4 cut(s) 28, 383, 418, 472
LguI GCTCTTC 1 cut(s) 74
LpnPI CCDG 2 cut(s) 337, 426
MaeI CTAG 1 cut(s) 39
MaeII ACGT 1 cut(s) 574
MalI GATC 4 cut(s) 30, 385, 420, 474
MboI GATC 4 cut(s) 28, 383, 418, 472
MboII GAAGA 2 cut(s) 91, 691
MfeI CAATTG 1 cut(s) 674
MflI RGATCY 2 cut(s) 383, 418
MhlI GDGCHC 1 cut(s) 619
MluCI AATT 6 cut(s) 55, 64, 102, 407, 503, 674
MmeI TCCRAC 2 cut(s) 192, 585
MnlI CCTC 2 cut(s) 637, 659
MseI TTAA 4 cut(s) 188, 284, 320, 702
MunI CAATTG 1 cut(s) 674
MwoI GCNNNNNNNGC 1 cut(s) 134
NdeII GATC 4 cut(s) 28, 383, 418, 472
NlaIII CATG 3 cut(s) 132, 137, 215
NspI RCATGY 1 cut(s) 137
NspV TTCGAA 1 cut(s) 59
PaeI GCATGC 1 cut(s) 137
PciSI GCTCTTC 1 cut(s) 74
PkrI GCNGC 1 cut(s) 672
PmaCI CACGTG 1 cut(s) 575
PmlI CACGTG 1 cut(s) 575
Ppu21I YACGTR 1 cut(s) 575
PspCI CACGTG 1 cut(s) 575
PspPI GGNCC 1 cut(s) 668
PsuI RGATCY 2 cut(s) 383, 418
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SapI GCTCTTC 1 cut(s) 74
SaqAI TTAA 4 cut(s) 188, 284, 320, 702
SatI GCNGC 1 cut(s) 671
Sau3AI GATC 4 cut(s) 28, 383, 418, 472
Sau96I GGNCC 1 cut(s) 668
SduI GDGCHC 1 cut(s) 619
SetI ASST 8 cut(s) 102, 188, 194, 313, 442, 577, 601, 708
SfuI TTCGAA 1 cut(s) 59
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SphI GCATGC 1 cut(s) 137
Sse9I AATT 6 cut(s) 55, 64, 102, 407, 503, 674
SsiI CCGC 2 cut(s) 558, 671
SspI AATATT 2 cut(s) 91, 119
SspMI CTAG 1 cut(s) 39
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 2 cut(s) 8, 20
TaiI ACGT 1 cut(s) 577
TaqI TCGA 1 cut(s) 59
TasI AATT 6 cut(s) 55, 64, 102, 407, 503, 674
TauI GCSGC 1 cut(s) 673
Tru1I TTAA 4 cut(s) 188, 284, 320, 702
Tru9I TTAA 4 cut(s) 188, 284, 320, 702
TspDTI ATGAA 2 cut(s) 57, 68
XapI RAATTY 2 cut(s) 55, 503
XceI RCATGY 1 cut(s) 137
XspI CTAG 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.