pycom13g18260

2S seed storage protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0017371
Physical Location & Seq
Reverse (-)
14354199 .. 14354873
675 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g18260.1

Sequence Viewer

Length: 675 bp
ATGTATGACGGAGCAAGGACTGCCGTAAGAACTTATGAAGGACAAACCAAAAGCTTCCCTATAATTGTAGGGTTACATCAAGGCTCATCTTTAAGTCCTTACCTTTTCGCATTGGTAATGGATGGGTTAACGAGATATATTCAAGATGGTATCCCTTGGTGTATGCTTTTCGCAACCGATATAGTGTTGATAGATGAAACTCAGGAAGGAGCAAATGCGAAGCTTAACCTTTGGAAAGAAGTGTTGGAATCTAAAGGTTTTCGCGTAAGTCGATCAAAGACAGAGTATATGGAGTGCAAGTTCAATGCAAACGGAGGCTCAAATGAGTTAGAGATGAGGATTGGAGATCAAGAAGTACCAAAGAGCGACTGCTTTCACTACGTAGGATCTATCTTGCAAAAGAACAGAGAGTTGGATGGCGACCTCAACCATAGAATACAAGCTGGATGGATGAAGTGGAAGAGTGCATCCGGCGTGTTGTGCGATCGTCGTATGCCACTGAAACTCAAGGGAAAATTTTATAGAACAACAATAAGGCCAACAATGCTTTATAGCACGGAATGTTGGGCGGTGAAGCATCAACATGTACATAAAATGGGTGTAGCGGAGATGAGAATGCTTCGCTGGATGTGTGGGCACACGAGAAAAGATAAGATTAGGAATAAGGATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Tryp_alpha_amyl

Protein Analysis

225

Amino Acids

25.86

Weight (kDa)

9.27

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 12 - 130 3.3e-11 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 264
AciI CCGC 2 cut(s) 569, 605
AclWI GGATC 1 cut(s) 394
AcsI RAATTY 1 cut(s) 515
AfaI GTAC 2 cut(s) 357, 588
AflIII ACRYGT 1 cut(s) 583
AgsI TTSAA 2 cut(s) 143, 304
AjuI GAANNNNNNNTTGG 4 cut(s) 227, 259, 395, 427
AloI GAACNNNNNNTCC 2 cut(s) 284, 316
AluBI AGCT 3 cut(s) 54, 223, 443
AluI AGCT 3 cut(s) 54, 223, 443
AlwI GGATC 1 cut(s) 394
AoxI GGCC 1 cut(s) 536
ApoI RAATTY 1 cut(s) 515
AsuHPI GGTGA 1 cut(s) 583
BaeGI GKGCMC 1 cut(s) 639
BauI CACGAG 1 cut(s) 640
BccI CCATC 4 cut(s) 116, 140, 410, 441
BceAI ACGGC 1 cut(s) 8
BciVI GTATCC 1 cut(s) 161
BfuI GTATCC 1 cut(s) 161
BmsI GCATC 2 cut(s) 476, 586
BpuEI CTTGAG 1 cut(s) 491
BsaAI YACGTR 1 cut(s) 382
BsaJI CCNNGG 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 6 cut(s) 127, 421, 452, 456, 467, 633
BseMII CTCAG 1 cut(s) 215
BseSI GKGCMC 1 cut(s) 639
Bsh1236I CGCG 1 cut(s) 264
Bsh1285I CGRYCG 1 cut(s) 487
BshFI GGCC 1 cut(s) 538
BsiEI CGRYCG 1 cut(s) 487
BsiSI CCGG 1 cut(s) 471
BsmI GAATGC 1 cut(s) 621
BsnI GGCC 1 cut(s) 538
Bsp1286I GDGCHC 1 cut(s) 639
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 4 cut(s) 272, 346, 386, 484
BspACI CCGC 2 cut(s) 569, 605
BspANI GGCC 1 cut(s) 538
BspCNI CTCAG 1 cut(s) 214
BspFNI CGCG 1 cut(s) 264
BspPI GGATC 1 cut(s) 394
BsrGI TGTACA 1 cut(s) 586
BssECI CCNNGG 1 cut(s) 155
BssMI GATC 4 cut(s) 272, 346, 386, 484
BssSI CACGAG 1 cut(s) 640
BssT1I CCWWGG 1 cut(s) 155
Bst2BI CACGAG 1 cut(s) 640
Bst6I CTCTTC 1 cut(s) 455
BstAPI GCANNNNNTGC 1 cut(s) 20
BstAUI TGTACA 1 cut(s) 586
BstBAI YACGTR 1 cut(s) 382
BstDEI CTNAG 1 cut(s) 201
BstF5I GGATG 6 cut(s) 127, 421, 452, 456, 467, 633
BstFNI CGCG 1 cut(s) 264
BstKTI GATC 4 cut(s) 275, 349, 389, 487
BstMBI GATC 4 cut(s) 272, 346, 386, 484
BstMCI CGRYCG 1 cut(s) 487
BstMWI GCNNNNNNNGC 3 cut(s) 20, 480, 544
BstNSI RCATGY 1 cut(s) 587
BstSLI GKGCMC 1 cut(s) 639
BstSNI TACGTA 1 cut(s) 382
BstUI CGCG 1 cut(s) 264
BstX2I RGATCY 1 cut(s) 386
BstYI RGATCY 1 cut(s) 386
BsuI GTATCC 1 cut(s) 161
BsuRI GGCC 1 cut(s) 538
BtsCI GGATG 6 cut(s) 127, 421, 452, 456, 467, 633
BtsIMutI CAGTG 1 cut(s) 497
Csp6I GTAC 2 cut(s) 356, 587
CviAII CATG 1 cut(s) 584
CviJI RGCY 6 cut(s) 54, 84, 223, 318, 443, 538
CviKI_1 RGCY 6 cut(s) 54, 84, 223, 318, 443, 538
CviQI GTAC 2 cut(s) 356, 587
DdeI CTNAG 1 cut(s) 201
DpnI GATC 4 cut(s) 274, 348, 388, 486
DpnII GATC 4 cut(s) 272, 346, 386, 484
Eam1104I CTCTTC 1 cut(s) 455
EarI CTCTTC 1 cut(s) 455
Eco105I TACGTA 1 cut(s) 382
Eco130I CCWWGG 1 cut(s) 155
Eco32I GATATC 1 cut(s) 670
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 1 cut(s) 155
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 1 cut(s) 587
FatI CATG 1 cut(s) 583
FokI GGATG 6 cut(s) 134, 428, 454, 459, 463, 640
HaeIII GGCC 1 cut(s) 538
HapII CCGG 1 cut(s) 471
Hin1II CATG 1 cut(s) 587
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HindIII AAGCTT 2 cut(s) 52, 221
HinfI GANTC 1 cut(s) 248
HpaI GTTAAC 1 cut(s) 129
HpaII CCGG 1 cut(s) 471
HphI GGTGA 1 cut(s) 583
Hpy166II GTNNAC 1 cut(s) 129
Hpy188I TCNGA 1 cut(s) 674
Hpy188III TCNNGA 3 cut(s) 143, 203, 350
Hpy8I GTNNAC 1 cut(s) 129
Hpy99I CGWCG 1 cut(s) 492
HpyAV CCTTC 2 cut(s) 32, 200
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 4 cut(s) 297, 308, 397, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 480, 544
HpyF3I CTNAG 1 cut(s) 201
HpySE526I ACGT 1 cut(s) 381
Hsp92II CATG 1 cut(s) 587
KspAI GTTAAC 1 cut(s) 129
Kzo9I GATC 4 cut(s) 272, 346, 386, 484
LmnI GCTCC 2 cut(s) 11, 209
LpnPI CCDG 4 cut(s) 188, 429, 484, 610
LweI GCATC 2 cut(s) 476, 586
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 72
MalI GATC 4 cut(s) 274, 348, 388, 486
MboI GATC 4 cut(s) 272, 346, 386, 484
MboII GAAGA 1 cut(s) 472
MflI RGATCY 1 cut(s) 386
MhlI GDGCHC 1 cut(s) 639
MluCI AATT 2 cut(s) 63, 515
MmeI TCCRAC 2 cut(s) 225, 393
MnlI CCTC 3 cut(s) 308, 330, 434
MseI TTAA 3 cut(s) 92, 128, 225
MslI CAYNNNNRTG 1 cut(s) 582
MspI CCGG 1 cut(s) 471
Mva1269I GAATGC 1 cut(s) 621
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 3 cut(s) 20, 480, 544
NdeII GATC 4 cut(s) 272, 346, 386, 484
NlaIII CATG 1 cut(s) 587
NspI RCATGY 1 cut(s) 587
PciI ACATGT 1 cut(s) 583
PcsI WCGNNNNNNNCGW 1 cut(s) 268
PctI GAATGC 1 cut(s) 621
PfeI GAWTC 1 cut(s) 248
Ple19I CGATCG 1 cut(s) 487
Ppu21I YACGTR 1 cut(s) 382
PscI ACATGT 1 cut(s) 583
PsuI RGATCY 1 cut(s) 386
PvuI CGATCG 1 cut(s) 487
RsaI GTAC 2 cut(s) 357, 588
RsaNI GTAC 2 cut(s) 356, 587
RseI CAYNNNNRTG 1 cut(s) 582
SaqAI TTAA 3 cut(s) 92, 128, 225
Sau3AI GATC 4 cut(s) 272, 346, 386, 484
SduI GDGCHC 1 cut(s) 639
SetI ASST 8 cut(s) 56, 105, 225, 231, 259, 384, 426, 445
SfaNI GCATC 2 cut(s) 476, 586
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
SnaBI TACGTA 1 cut(s) 382
Sse9I AATT 2 cut(s) 63, 515
SsiI CCGC 2 cut(s) 569, 605
StyI CCWWGG 1 cut(s) 155
TaiI ACGT 1 cut(s) 384
TaqI TCGA 1 cut(s) 271
TasI AATT 2 cut(s) 63, 515
TatI WGTACW 1 cut(s) 586
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 3 cut(s) 92, 128, 225
Tru9I TTAA 3 cut(s) 92, 128, 225
TscAI CASTG 1 cut(s) 504
TspDTI ATGAA 3 cut(s) 51, 210, 467
TspGWI ACGGA 3 cut(s) 24, 327, 572
TspRI CASTG 1 cut(s) 504
XapI RAATTY 1 cut(s) 515
XceI RCATGY 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.