pycom06g10440

30S ribosomal protein S6 alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014121
Physical Location & Seq
Reverse (-)
15767861 .. 15769863
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
ATGGCTTCCACTTCGTCCCTCATTTCGTCTCTTACCCCTACATCAAATTTCCCCTTTTTCCCCAAAACAATTTCCCAAATCCCATCTCTACCCCAAACCGTCTCCTTCCCTCTCTTTAACCGAAACGGACCGAATCCCAGTTCGCGCCTAACCTCCAATGTCCAGTCCCTGGAGTTCTCGGGCTCGTTTTTTGAGGGCGGGTTTCAATCGGAGGACGACCCGACTTCCCCGCCCGGGTCGGGCTTGACCGCCGTGGAGGAGAAGGAGGAGCCCCCGTGCCCACCGGGGCTCCGACAGTACGAGACCATGGCGGTGCTCAGACCCGACATGTCCGAGGATGAAAGACTTACTCTTACCCAGAAGTACGAGGAGTTGCTAATAGCCGGGGGTGGCATGTACGTGGAGGTCTTCAACAGAGGGGTAATTCCACTAGCCTATGGCATCCAGAAGAAAAACAAAGCCGGAGAGTCCAACACTTACCTGGATGGTATCTACCTCCTCTTTACCTACTTCACAAAACCCGAATCTCTCGAAGTTCTTGAAGCAAATCTGAAAGCTGATGATAATGTCATCCGATCGATGAGCTTCAAAATAAGGAAGAGGAAGTACTAG

Protein Analysis

204

Amino Acids

22.56

Weight (kDa)

5.06

Isoelectric Point (pI)

53.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S6 PF01250 99 - 197 2.5e-18 Ribosomal protein S6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014121)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64510 AT1G64510
fragaria_vesca FvH4_5g11660
malus_domestica MD06G1111400.v1.1 MD14G1132700.v1.1
prunus_persica Prupe.5G124200_v2.0.a1
pyrus_communis pycom06g10440
rosa_chinensis RchiOBHm_Chr7g0186131
rosa_laevigata RLG00000004861
rosa_multiflora Rmu_sc0000194.1_g000031 Rmu_sc0029813.1_g000001
rosa_roxburghii Rroxscaffold_3G00268400
rosa_rugosa Rorug06G0472200
rosa_samantha Rh7AG069300 Rh7BG085300 Rh7CG078800
rosa_wichuraiana Rw7G006560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 169
AccII CGCG 1 cut(s) 145
AciI CCGC 4 cut(s) 198, 230, 249, 311
AcsI RAATTY 1 cut(s) 46
AfaI GTAC 4 cut(s) 299, 365, 398, 608
AfiI CCNNNNNNNGG 3 cut(s) 169, 234, 239
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 4 cut(s) 206, 412, 542, 589
AjnI CCWGG 2 cut(s) 168, 480
AluBI AGCT 2 cut(s) 557, 585
AluI AGCT 2 cut(s) 557, 585
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 3 cut(s) 33, 106, 296
AlwNI CAGNNNCTG 1 cut(s) 169
Ama87I CYCGRG 2 cut(s) 178, 233
ApoI RAATTY 1 cut(s) 46
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 1 cut(s) 128
AsuC2I CCSGG 4 cut(s) 234, 235, 285, 385
AvaI CYCGRG 2 cut(s) 178, 233
AvaII GGWCC 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 281
BanII GRGCYC 3 cut(s) 185, 273, 291
BarI GAAGNNNNNNTAC 1 cut(s) 590
BbsI GAAGAC 1 cut(s) 400
Bbv12I GWGCWC 1 cut(s) 318
BccI CCATC 2 cut(s) 91, 479
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 2 cut(s) 170, 482
BcnI CCSGG 4 cut(s) 234, 235, 285, 385
BcoDI GTCTC 3 cut(s) 33, 106, 296
BfaI CTAG 2 cut(s) 431, 610
BmcAI AGTACT 1 cut(s) 608
Bme1390I CCNGG 6 cut(s) 170, 234, 235, 285, 385, 482
Bme18I GGWCC 1 cut(s) 128
BmeT110I CYCGRG 2 cut(s) 178, 233
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 2 cut(s) 270, 290
BmrFI CCNGG 6 cut(s) 170, 234, 235, 285, 385, 482
BmrI ACTGGG 1 cut(s) 132
BmsI GCATC 1 cut(s) 450
BmuI ACTGGG 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 400
BpmI CTGGAG 1 cut(s) 191
BpuMI CCSGG 4 cut(s) 234, 235, 285, 385
Bsa29I ATCGAT 1 cut(s) 578
BsaAI YACGTR 1 cut(s) 400
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 7 cut(s) 168, 233, 252, 284, 306, 333, 384
BsaXI ACNNNNNCTCC 4 cut(s) 260, 273, 290, 303
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 234, 239
Bse1I ACTGG 2 cut(s) 138, 163
BseBI CCWGG 2 cut(s) 170, 482
BseCI ATCGAT 1 cut(s) 578
BseDI CCNNGG 7 cut(s) 168, 233, 252, 284, 306, 333, 384
BseGI GGATG 4 cut(s) 343, 441, 490, 570
BseLI CCNNNNNNNGG 3 cut(s) 169, 234, 239
BseMII CTCAG 1 cut(s) 331
BseNI ACTGG 2 cut(s) 138, 163
BseRI GAGGAG 4 cut(s) 272, 281, 383, 488
BseSI GKGCMC 1 cut(s) 281
Bsh1236I CGCG 1 cut(s) 145
Bsh1285I CGRYCG 1 cut(s) 578
BshVI ATCGAT 1 cut(s) 578
BsiEI CGRYCG 1 cut(s) 578
BsiHKAI GWGCWC 1 cut(s) 318
BsiHKCI CYCGRG 2 cut(s) 178, 233
BsiSI CCGG 4 cut(s) 234, 284, 384, 462
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 3 cut(s) 169, 234, 239
BsmAI GTCTC 3 cut(s) 33, 106, 296
BsmBI CGTCTC 2 cut(s) 33, 106
BsmFI GGGAC 1 cut(s) 151
Bso31I GGTCTC 1 cut(s) 296
BsoBI CYCGRG 2 cut(s) 178, 233
Bsp1286I GDGCHC 5 cut(s) 185, 273, 281, 291, 318
Bsp143I GATC 1 cut(s) 575
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 4 cut(s) 198, 230, 249, 311
BspCNI CTCAG 1 cut(s) 330
BspDI ATCGAT 1 cut(s) 578
BspFNI CGCG 1 cut(s) 145
BspLI GGNNCC 2 cut(s) 270, 290
BspTNI GGTCTC 1 cut(s) 296
BsrI ACTGG 2 cut(s) 138, 163
BssECI CCNNGG 7 cut(s) 168, 233, 252, 284, 306, 333, 384
BssMI GATC 1 cut(s) 575
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 2 cut(s) 170, 482
Bst4CI ACNGT 2 cut(s) 100, 297
Bst6I CTCTTC 1 cut(s) 593
BstBAI YACGTR 1 cut(s) 400
BstDEI CTNAG 1 cut(s) 317
BstDSI CCRYGG 2 cut(s) 252, 306
BstF5I GGATG 4 cut(s) 343, 441, 490, 570
BstFNI CGCG 1 cut(s) 145
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 1 cut(s) 578
BstMAI GTCTC 3 cut(s) 33, 106, 296
BstMBI GATC 1 cut(s) 575
BstMCI CGRYCG 1 cut(s) 578
BstNI CCWGG 2 cut(s) 170, 482
BstNSI RCATGY 2 cut(s) 331, 397
BstSCI CCNGG 6 cut(s) 168, 232, 233, 283, 383, 480
BstSLI GKGCMC 1 cut(s) 281
BstUI CGCG 1 cut(s) 145
BstV2I GAAGAC 1 cut(s) 400
Bsu15I ATCGAT 1 cut(s) 578
BsuTUI ATCGAT 1 cut(s) 578
BtgI CCRYGG 2 cut(s) 252, 306
BtsCI GGATG 4 cut(s) 343, 441, 490, 570
CaiI CAGNNNCTG 1 cut(s) 169
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 1 cut(s) 128
Cfr9I CCCGGG 1 cut(s) 233
ClaI ATCGAT 1 cut(s) 578
CpoI CGGWCCG 1 cut(s) 128
Csp6I GTAC 4 cut(s) 298, 364, 397, 607
CspI CGGWCCG 1 cut(s) 128
CviAII CATG 3 cut(s) 307, 328, 394
CviQI GTAC 4 cut(s) 298, 364, 397, 607
DdeI CTNAG 1 cut(s) 317
DpnI GATC 1 cut(s) 577
DpnII GATC 1 cut(s) 575
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 3 cut(s) 185, 273, 291
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 128
Eco88I CYCGRG 2 cut(s) 178, 233
EcoRII CCWGG 2 cut(s) 168, 480
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 3 cut(s) 185, 273, 291
ErhI CCWWGG 1 cut(s) 306
Esp3I CGTCTC 2 cut(s) 33, 106
FaeI CATG 3 cut(s) 310, 331, 397
FaiI YATR 4 cut(s) 308, 329, 395, 438
FaqI GGGAC 1 cut(s) 151
FatI CATG 3 cut(s) 306, 327, 393
FauI CCCGC 2 cut(s) 191, 237
FokI GGATG 4 cut(s) 350, 428, 497, 557
FriOI GRGCYC 3 cut(s) 185, 273, 291
FspBI CTAG 2 cut(s) 431, 610
GlaI GCGC 1 cut(s) 146
GsuI CTGGAG 1 cut(s) 191
HapII CCGG 4 cut(s) 234, 284, 384, 462
HhaI GCGC 1 cut(s) 147
Hin1II CATG 3 cut(s) 310, 331, 397
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HinfI GANTC 3 cut(s) 133, 467, 524
HpaII CCGG 4 cut(s) 234, 284, 384, 462
Hpy188I TCNGA 6 cut(s) 211, 293, 320, 334, 552, 575
Hpy188III TCNNGA 3 cut(s) 445, 530, 539
HpyAV CCTTC 2 cut(s) 115, 256
HpyCH4III ACNGT 2 cut(s) 100, 297
HpyCH4IV ACGT 1 cut(s) 399
HpyF3I CTNAG 1 cut(s) 317
HpySE526I ACGT 1 cut(s) 399
Hsp92II CATG 3 cut(s) 310, 331, 397
HspAI GCGC 1 cut(s) 145
Kzo9I GATC 1 cut(s) 575
LmnI GCTCC 2 cut(s) 268, 294
LweI GCATC 1 cut(s) 450
MaeI CTAG 2 cut(s) 431, 610
MaeII ACGT 1 cut(s) 399
MalI GATC 1 cut(s) 577
MboI GATC 1 cut(s) 575
MboII GAAGA 3 cut(s) 400, 460, 610
MhlI GDGCHC 5 cut(s) 185, 273, 281, 291, 318
MluCI AATT 3 cut(s) 46, 69, 423
MlyI GAGTC 1 cut(s) 476
MmeI TCCRAC 2 cut(s) 316, 495
MseI TTAA 1 cut(s) 117
MslI CAYNNNNRTG 2 cut(s) 311, 398
MspI CCGG 4 cut(s) 234, 284, 384, 462
MspR9I CCNGG 6 cut(s) 170, 234, 235, 285, 385, 482
MvaI CCWGG 2 cut(s) 170, 482
MvnI CGCG 1 cut(s) 145
NciI CCSGG 4 cut(s) 234, 235, 285, 385
NcoI CCATGG 1 cut(s) 306
NdeII GATC 1 cut(s) 575
NlaIII CATG 3 cut(s) 310, 331, 397
NlaIV GGNNCC 2 cut(s) 270, 290
NspI RCATGY 2 cut(s) 331, 397
PciI ACATGT 1 cut(s) 327
PfeI GAWTC 2 cut(s) 133, 524
PflMI CCANNNNNTGG 1 cut(s) 169
Ple19I CGATCG 1 cut(s) 578
PleI GAGTC 1 cut(s) 475
PpsI GAGTC 1 cut(s) 475
Ppu21I YACGTR 1 cut(s) 400
PscI ACATGT 1 cut(s) 327
Psp6I CCWGG 2 cut(s) 168, 480
PspGI CCWGG 2 cut(s) 168, 480
PspN4I GGNNCC 2 cut(s) 270, 290
PspPI GGNCC 1 cut(s) 128
PstNI CAGNNNCTG 1 cut(s) 169
PvuI CGATCG 1 cut(s) 578
RsaI GTAC 4 cut(s) 299, 365, 398, 608
RsaNI GTAC 4 cut(s) 298, 364, 397, 607
RseI CAYNNNNRTG 2 cut(s) 311, 398
Rsr2I CGGWCCG 1 cut(s) 128
RsrII CGGWCCG 1 cut(s) 128
SaqAI TTAA 1 cut(s) 117
Sau3AI GATC 1 cut(s) 575
Sau96I GGNCC 1 cut(s) 128
ScaI AGTACT 1 cut(s) 608
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 6 cut(s) 170, 234, 235, 285, 385, 482
SduI GDGCHC 5 cut(s) 185, 273, 281, 291, 318
SetI ASST 8 cut(s) 155, 402, 408, 483, 498, 509, 559, 587
SfaNI GCATC 1 cut(s) 450
SinI GGWCC 1 cut(s) 128
SmaI CCCGGG 1 cut(s) 235
SmiMI CAYNNNNRTG 2 cut(s) 311, 398
Sse9I AATT 3 cut(s) 46, 69, 423
SsiI CCGC 4 cut(s) 198, 230, 249, 311
SspMI CTAG 2 cut(s) 431, 610
StyD4I CCNGG 6 cut(s) 168, 232, 233, 283, 383, 480
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 100, 297
TaiI ACGT 1 cut(s) 402
TaqI TCGA 2 cut(s) 531, 578
TaqII GACCGA 1 cut(s) 145
TasI AATT 3 cut(s) 46, 69, 423
TatI WGTACW 1 cut(s) 606
TfiI GAWTC 2 cut(s) 133, 524
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TspDTI ATGAA 1 cut(s) 354
TspGWI ACGGA 1 cut(s) 141
TspMI CCCGGG 1 cut(s) 233
Van91I CCANNNNNTGG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 1 cut(s) 46
XceI RCATGY 2 cut(s) 331, 397
XcmI CCANNNNNNNNNTGG 1 cut(s) 478
XmaI CCCGGG 1 cut(s) 233
XspI CTAG 2 cut(s) 431, 610
ZrmI AGTACT 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.