RLG00000004861

30S ribosomal protein S6 alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014121
Physical Location & Seq
Forward (+)
63165566 .. 63167267
1702 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004861

Sequence Viewer

Length: 627 bp
ATGGCAGCTTCAACTTCCTCCCCCATTTCCACCACTTGGAATTTCCTGTTTTGCCCCAAAACTCTCTCCCAAATCCCATCCCTACCCCAATCCCTCTTCTTCCCTCACACAACCGCAAAGCCTCTCGTTCACCGGACCCGACCCGGCCGCAATTCGGGCCTGACCGTCAATGTCCAGTCGCTGGAGTTCTCCGGCTCATTCTTCGAGGGCGGCGGGTTCCAGTCGGAGGACGACCCGAATTCTCCGTCCGGGCCCGGCCTAACCGCCGCGGAGGACAAGGAGGACCCGCCGTGCCCGCCGGGGCTCCGGCAGTATGAGACGATGGCGGTTCTCAGACCCGATATGTCTGAGGATGAAAGACTGGCTCTTACCCAGAAGTATGAGGAGTTGCTTATAGCTGGAGGTGGCATGTATGTGGAGGTCTTCAACAGAGGAGTGATTCCACTAGCCTATAGCATCCAGAAGAAAAACAAAGCTGGAGAATCCAACACTTACCTGGATGGCATCTACCTACTCTTTACCTACTTCACAAAACCTGAGTCTCTCGAGTTTCTTGAACAGAATTTGAAAGCTGATGACAATGTCATCCGATCTATGAGTTTCAAAATAAGGAAGAGGAAGTACTAG

Protein Analysis

209

Amino Acids

23.2

Weight (kDa)

5.78

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S6 PF01250 104 - 202 9.3e-19 Ribosomal protein S6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014121)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64510 AT1G64510
fragaria_vesca FvH4_5g11660
malus_domestica MD06G1111400.v1.1 MD14G1132700.v1.1
prunus_persica Prupe.5G124200_v2.0.a1
pyrus_communis pycom06g10440
rosa_chinensis RchiOBHm_Chr7g0186131
rosa_laevigata RLG00000004861
rosa_multiflora Rmu_sc0000194.1_g000031 Rmu_sc0029813.1_g000001
rosa_roxburghii Rroxscaffold_3G00268400
rosa_rugosa Rorug06G0472200
rosa_samantha Rh7AG069300 Rh7BG085300 Rh7CG078800
rosa_wichuraiana Rw7G006560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 36, 181
AccII CGCG 1 cut(s) 269
AcoI YGGCCR 1 cut(s) 145
AcsI RAATTY 3 cut(s) 40, 238, 562
AfaI GTAC 1 cut(s) 623
AfiI CCNNNNNNNGG 4 cut(s) 36, 154, 181, 226
AgsI TTSAA 5 cut(s) 12, 427, 557, 568, 604
AjnI CCWGG 1 cut(s) 495
AluBI AGCT 4 cut(s) 8, 398, 476, 572
AluI AGCT 4 cut(s) 8, 398, 476, 572
Alw26I GTCTC 2 cut(s) 311, 546
AlwNI CAGNNNCTG 1 cut(s) 181
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 4 cut(s) 145, 157, 251, 256
ApaI GGGCCC 1 cut(s) 255
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 3 cut(s) 40, 238, 562
ArsI GACNNNNNNTTYG 4 cut(s) 230, 262, 524, 556
AspS9I GGNCC 5 cut(s) 135, 157, 251, 252, 283
AsuC2I CCSGG 4 cut(s) 144, 250, 255, 300
AsuHPI GGTGA 1 cut(s) 122
AvaI CYCGRG 1 cut(s) 545
AvaII GGWCC 2 cut(s) 135, 283
BaeGI GKGCMC 2 cut(s) 255, 296
BanII GRGCYC 2 cut(s) 255, 306
BarI GAAGNNNNNNTAC 1 cut(s) 605
BbsI GAAGAC 1 cut(s) 415
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 85, 316, 494
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 1 cut(s) 497
BcnI CCSGG 4 cut(s) 144, 250, 255, 300
BcoDI GTCTC 2 cut(s) 311, 546
BfaI CTAG 2 cut(s) 446, 625
BfmI CTRYAG 1 cut(s) 451
BisI GCNGC 4 cut(s) 6, 148, 211, 267
BlsI GCNGC 4 cut(s) 7, 149, 212, 268
BmcAI AGTACT 1 cut(s) 623
Bme1390I CCNGG 5 cut(s) 144, 250, 255, 300, 497
Bme18I GGWCC 2 cut(s) 135, 283
BmeT110I CYCGRG 1 cut(s) 545
BmgT120I GGNCC 5 cut(s) 135, 157, 251, 252, 283
BmiI GGNNCC 5 cut(s) 137, 218, 253, 285, 305
BmrFI CCNGG 5 cut(s) 144, 250, 255, 300, 497
BmsI GCATC 2 cut(s) 465, 513
BpiI GAAGAC 1 cut(s) 415
BpmI CTGGAG 3 cut(s) 203, 420, 498
BpuMI CCSGG 4 cut(s) 144, 250, 255, 300
BsaJI CCNNGG 2 cut(s) 267, 299
BsaWI WCCGGW 1 cut(s) 132
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 154, 181, 226
Bse1I ACTGG 3 cut(s) 175, 220, 366
BseBI CCWGG 1 cut(s) 497
BseDI CCNNGG 2 cut(s) 267, 299
BseGI GGATG 5 cut(s) 77, 358, 456, 505, 585
BseLI CCNNNNNNNGG 4 cut(s) 36, 154, 181, 226
BseMII CTCAG 3 cut(s) 339, 346, 528
BseNI ACTGG 3 cut(s) 175, 220, 366
BseRI GAGGAG 2 cut(s) 398, 447
BseSI GKGCMC 2 cut(s) 255, 296
BseX3I CGGCCG 1 cut(s) 145
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 1 cut(s) 269
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 4 cut(s) 147, 159, 253, 258
BsiEI CGRYCG 1 cut(s) 148
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 7 cut(s) 133, 144, 192, 249, 255, 299, 307
BslI CCNNNNNNNGG 4 cut(s) 36, 154, 181, 226
BsmAI GTCTC 2 cut(s) 311, 546
BsmBI CGTCTC 1 cut(s) 311
BsnI GGCC 4 cut(s) 147, 159, 253, 258
BsoBI CYCGRG 1 cut(s) 545
Bsp120I GGGCCC 1 cut(s) 251
Bsp1286I GDGCHC 3 cut(s) 255, 296, 306
Bsp143I GATC 1 cut(s) 590
BspANI GGCC 4 cut(s) 147, 159, 253, 258
BspCNI CTCAG 3 cut(s) 340, 345, 529
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 5 cut(s) 137, 218, 253, 285, 305
BsrI ACTGG 3 cut(s) 175, 220, 366
BssECI CCNNGG 2 cut(s) 267, 299
BssMI GATC 1 cut(s) 590
Bst2UI CCWGG 1 cut(s) 497
Bst4CI ACNGT 1 cut(s) 166
Bst6I CTCTTC 2 cut(s) 101, 608
BstC8I GCNNGC 1 cut(s) 296
BstDEI CTNAG 3 cut(s) 332, 348, 537
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 5 cut(s) 77, 358, 456, 505, 585
BstFNI CGCG 1 cut(s) 269
BstKTI GATC 1 cut(s) 593
BstMAI GTCTC 2 cut(s) 311, 546
BstMBI GATC 1 cut(s) 590
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 2 cut(s) 156, 295
BstNI CCWGG 1 cut(s) 497
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 5 cut(s) 142, 248, 253, 298, 495
BstSFI CTRYAG 1 cut(s) 451
BstSLI GKGCMC 2 cut(s) 255, 296
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 415
BstZI CGGCCG 1 cut(s) 145
BsuRI GGCC 4 cut(s) 147, 159, 253, 258
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 5 cut(s) 77, 358, 456, 505, 585
Cac8I GCNNGC 1 cut(s) 296
CaiI CAGNNNCTG 1 cut(s) 181
Cfr13I GGNCC 5 cut(s) 135, 157, 251, 252, 283
Cfr42I CCGCGG 1 cut(s) 270
Csp6I GTAC 1 cut(s) 622
CviAII CATG 1 cut(s) 409
CviQI GTAC 1 cut(s) 622
DdeI CTNAG 3 cut(s) 332, 348, 537
DpnI GATC 1 cut(s) 592
DpnII GATC 1 cut(s) 590
EaeI YGGCCR 1 cut(s) 145
EagI CGGCCG 1 cut(s) 145
Eam1104I CTCTTC 2 cut(s) 101, 608
EarI CTCTTC 2 cut(s) 101, 608
EclXI CGGCCG 1 cut(s) 145
Eco24I GRGCYC 2 cut(s) 255, 306
Eco47I GGWCC 2 cut(s) 135, 283
Eco52I CGGCCG 1 cut(s) 145
Eco88I CYCGRG 1 cut(s) 545
EcoO109I RGGNCCY 1 cut(s) 283
EcoRI GAATTC 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 495
EcoT38I GRGCYC 2 cut(s) 255, 306
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 1 cut(s) 412
FaiI YATR 8 cut(s) 315, 344, 381, 395, 410, 414, 453, 596
FatI CATG 1 cut(s) 408
FauI CCCGC 3 cut(s) 206, 294, 303
Fnu4HI GCNGC 4 cut(s) 6, 148, 211, 267
FokI GGATG 5 cut(s) 64, 365, 443, 512, 572
FriOI GRGCYC 2 cut(s) 255, 306
Fsp4HI GCNGC 4 cut(s) 6, 148, 211, 267
FspBI CTAG 2 cut(s) 446, 625
GluI GCNGC 4 cut(s) 6, 148, 211, 267
GsuI CTGGAG 3 cut(s) 203, 420, 498
HaeIII GGCC 4 cut(s) 147, 159, 253, 258
HapII CCGG 7 cut(s) 133, 144, 192, 249, 255, 299, 307
Hin1II CATG 1 cut(s) 412
HinfI GANTC 3 cut(s) 439, 482, 539
HpaII CCGG 7 cut(s) 133, 144, 192, 249, 255, 299, 307
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 4 cut(s) 226, 335, 349, 590
Hpy188III TCNNGA 3 cut(s) 460, 545, 554
Hpy8I GTNNAC 1 cut(s) 130
HpyCH4III ACNGT 1 cut(s) 166
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 295
HpyF3I CTNAG 3 cut(s) 332, 348, 537
Hsp92II CATG 1 cut(s) 412
KspI CCGCGG 1 cut(s) 270
Kzo9I GATC 1 cut(s) 590
LmnI GCTCC 1 cut(s) 309
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 2 cut(s) 465, 513
MaeI CTAG 2 cut(s) 446, 625
MalI GATC 1 cut(s) 592
MboI GATC 1 cut(s) 590
MboII GAAGA 6 cut(s) 88, 91, 193, 415, 475, 625
MhlI GDGCHC 3 cut(s) 255, 296, 306
MluCI AATT 4 cut(s) 40, 151, 238, 562
MlyI GAGTC 1 cut(s) 548
MmeI TCCRAC 2 cut(s) 204, 510
MslI CAYNNNNRTG 1 cut(s) 413
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 7 cut(s) 133, 144, 192, 249, 255, 299, 307
MspR9I CCNGG 5 cut(s) 144, 250, 255, 300, 497
MvaI CCWGG 1 cut(s) 497
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 156, 295
NciI CCSGG 4 cut(s) 144, 250, 255, 300
NdeII GATC 1 cut(s) 590
NlaIII CATG 1 cut(s) 412
NlaIV GGNNCC 5 cut(s) 137, 218, 253, 285, 305
NspI RCATGY 1 cut(s) 412
PaeR7I CTCGAG 1 cut(s) 545
PfeI GAWTC 2 cut(s) 439, 482
PflFI GACNNNGTC 1 cut(s) 581
PflMI CCANNNNNTGG 2 cut(s) 36, 181
PkrI GCNGC 4 cut(s) 7, 149, 212, 268
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
PpuMI RGGWCCY 1 cut(s) 283
Psp5II RGGWCCY 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 495
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 5 cut(s) 137, 218, 253, 285, 305
PspOMI GGGCCC 1 cut(s) 251
PspPI GGNCC 5 cut(s) 135, 157, 251, 252, 283
PspPPI RGGWCCY 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 181
PsyI GACNNNGTC 1 cut(s) 581
RsaI GTAC 1 cut(s) 623
RsaNI GTAC 1 cut(s) 622
RseI CAYNNNNRTG 1 cut(s) 413
SacII CCGCGG 1 cut(s) 270
SatI GCNGC 4 cut(s) 6, 148, 211, 267
Sau3AI GATC 1 cut(s) 590
Sau96I GGNCC 5 cut(s) 135, 157, 251, 252, 283
ScaI AGTACT 1 cut(s) 623
SchI GAGTC 1 cut(s) 548
ScrFI CCNGG 5 cut(s) 144, 250, 255, 300, 497
SduI GDGCHC 3 cut(s) 255, 296, 306
SfaNI GCATC 2 cut(s) 465, 513
SfcI CTRYAG 1 cut(s) 451
Sfr274I CTCGAG 1 cut(s) 545
Sfr303I CCGCGG 1 cut(s) 270
SgrBI CCGCGG 1 cut(s) 270
SinI GGWCC 2 cut(s) 135, 283
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 4 cut(s) 40, 151, 238, 562
SspMI CTAG 2 cut(s) 446, 625
StyD4I CCNGG 5 cut(s) 142, 248, 253, 298, 495
TaaI ACNGT 1 cut(s) 166
TaqI TCGA 2 cut(s) 204, 546
TasI AATT 4 cut(s) 40, 151, 238, 562
TatI WGTACW 1 cut(s) 621
TauI GCSGC 3 cut(s) 150, 213, 269
TfiI GAWTC 2 cut(s) 439, 482
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 1 cut(s) 369
TspGWI ACGGA 1 cut(s) 234
Tth111I GACNNNGTC 1 cut(s) 581
Van91I CCANNNNNTGG 2 cut(s) 36, 181
VpaK11BI GGWCC 2 cut(s) 135, 283
XapI RAATTY 3 cut(s) 40, 238, 562
XceI RCATGY 1 cut(s) 412
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XhoI CTCGAG 1 cut(s) 545
XspI CTAG 2 cut(s) 446, 625
ZrmI AGTACT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.