pycom06g10830
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009006
Physical Location & Seq
Forward (+)
16081931 .. 16085712
3782 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g10830.1

Sequence Viewer

Length: 2463 bp
ATGGCAGTGGCTTTGGTTGGAGGGGCTGCGATAGGAGCACTGTTTGGAGTACTGTATGATGTCGTAAAGGAATCGATGGGAAGGACTGTTATGCAGTACAAACCCCTCCTTGAAGATCTCAAGTTCACACTAGACTGTTTACGACCGCGGATCATCCAAGAGATAGGAGATCATAACGTGGCACTGGGTCTCCCAAACGATGACATAGAACGTCTCCAACAACAAATGGAGGAGGGTATAAAGCTCGTTGGCAGGTTATCGAAGCTTAGCATGTGGAACTGCTACGTCTTGTGCAGTTGCTGCAAATGCTCGGAGCCGAGCTACACCGATCGACTTATTCAATTGGATAGGTCTCTTAGAGCACTGTTAGAAGTGCTGAGGTTGCAGGAAGCCAGGGACTTGAAGGAGAACTTGCTTTTGAATAGGAAGATATACGAAAAACAAGATGAATTGGCAACAAGGCAGTCGGAAATACTGAAAATTGCAGGGGAGATACTGAGAGCGCAGCAGGAAGATAAGGGTGTGCAAGAGCTTTCAGGAAGTAATGCACAAACTGTGGTGCAGGAGCAAATTGGAGGCAGTGGTGTGCAGCAAGCGGCATCAATAAACAGAGGAGGAGCTGCTCTAGGAGCAGTGTTTGAGGTACTATTTAGTGCCGTTCAACAAACGAAGGAGAAGACGAGGATGTTTAAACGCATCCTGGGACATCTCGAGTCCACGCTAGACTCCATAAAGCCATGGATAGAAGAGATTGCAGAATATAACAAGGTATTGCATCTTACAGAGGAAGAACTAGAAAGTTTTAGAGTAGAGATGGAGAAGGGTGTAGAGCTCGTTCGCAAGTGCTCAAACGTTAGTCTTTGGGCAAGCAACAAAAAGTATGAATATACCAACAAACTTCTTGGATTAGATGAATCTCTTCAAAGATTGATAAATATACTGAGAGTGCAACTAGCAAGGGATGCGAAAGAAAGCTTGGATTCGGTAACTAATATAGCGACGGTGACCAAGCAAATTGAAGAGAGTGGTATGATACAACATGATCAAACAGAAGGTCAGCGTCCAGTAGTTGAACTTCCGTCCGATACGGTTGGTTTGGATGTATTGAGTGTGCAGGGAGCAGGGAAGATGACGAAAGCAAGGGAATTAGAGGAGGGGGCCAAGAAAATTGAAGGGAGTGGTGCTGTACAAAATCAAACTGAAATTCATGGTTCTTCAAGCATGGTTTCAATGGAAGAGTCTGAAACCGAATTTGAGCCACAACAAGTCTTCTCCTCGGAATTTGGCACAATCCACGAGAATATGCAGGGTAGCAGACCTGATACTTCTCCAAGGTTCTCCAATTGGTTCAGTAGTAGAGCCAAAAAAAGAGGAAAAAGTGGGAGCGAATATGGGCAGAAATATGTGGAAGTATTTTCGGATGCTTCGAGAGATAGGAGCGAGAGAAGATGCGGTTCTAGTCGAGGCATCTACTCCAATCCAAATAACTCAAGGCATGGGAGTGAGCCCGGGAGTATGAAGTTCACCATGGAAGAGATAAACAACGCCACAAAGAGCTTCTCTCCCTCTCGCAAGATTGGACAAGGTGGTTTCGGGACGGTTTATAAGGGGAGACTCGAAGACGGGACCTTTGTTGCAATCAAACGCGCTAAAAAGAGTGTATATGACAAGCATTTGGGAGTGGAATTCCAAAACGAGATTCAAATGCTGGCGCAGATGGAGCATTTGAGTTTGGTCAAGTTCTACGGGTACTTGGAGCATGAAGATGAAAAGATTGTTGTTGTCGAGTATGTTCCTAATGGAAACCTCAGACAACATTTGGATTGTAAGTTCGATTTGTGTTACTCTCTCTTCCTCTTCCTTTCTGTGTACCGGGTGTGCCTGCGTGTATCTGTGCACCTTGGCTCATCAATGCATCACCTTGAGTGCCTGAAATGTGATACAACGCGTAATGCAGATCATCCGATTATTCATAGAGACATAAACTCGTCCAACATTCTCTTAACGGAAAACTTCAGGGCCAAGGTAGCTGATTTTGGTTTTGCTAGGCTTGCTGCTGATAGCGACTCAGGTGCCACCCATGTTTCTACTCAAGTTAGGGGAACCGCTGGCTACTTGGACCCCGAGTACCTGAGAACCTATCAACTCACGGAGAAGAGTGATGTCTACTCATTTGGTGTGCTACTGGTTGAGCTAGTTACAGGCAGGCGTCCGATTGAACCTAAACGGGAAATCAAGGAACGAATAACTGCAAAATGGGCTATGAAGAAGTTTACCGATGGAGATGCTCTTTCGATTTTGGACCCGAGGTTAGGACAGACAGCAGCAAGTGGTCTAGCCATCGAAAAGATTCTTGAACTAGCATTACAATGCTTGGCTCCACGCGGACAGAACCGGCCTAGCATGAGGAGGTGCTGTGAGATCGCTCTGGAACATCCAGTTTTGCTTCCTCCGGGGATGTGA

Protein Analysis

821

Amino Acids

91.93

Weight (kDa)

6.29

Isoelectric Point (pI)

46.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 139 6.4e-18 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 205 - 331 2.9e-30 Arabidopsis broad-spectrum mildew resistance protein RPW8
Pkinase PF00069 522 - 807 8.6e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 523 - 806 1.1e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1605
Acc36I ACCTGC 1 cut(s) 243
AccB1I GGYRCC 1 cut(s) 2072
AccI GTMKAC 1 cut(s) 2166
AccII CGCG 4 cut(s) 148, 1647, 1948, 2385
AciI CCGC 6 cut(s) 146, 148, 596, 1452, 2106, 2385
AclI AACGTT 1 cut(s) 852
AclWI GGATC 1 cut(s) 158
AcsI RAATTY 4 cut(s) 1203, 1250, 1280, 1685
AcuI CTGAAG 1 cut(s) 1999
AcyI GRCGYC 1 cut(s) 2209
AfaI GTAC 7 cut(s) 51, 98, 645, 1188, 1751, 1871, 2129
AfiI CCNNNNNNNGG 1 cut(s) 2312
AflIII ACRYGT 1 cut(s) 1946
AjnI CCWGG 2 cut(s) 392, 699
AjuI GAANNNNNNNTTGG 4 cut(s) 959, 991, 1683, 1715
AloI GAACNNNNNNTCC 2 cut(s) 1814, 1846
Alw21I GWGCWC 5 cut(s) 40, 364, 834, 848, 1899
Alw26I GTCTC 5 cut(s) 194, 218, 357, 1606, 1971
Alw44I GTGCAC 1 cut(s) 1895
AlwI GGATC 1 cut(s) 158
AlwNI CAGNNNCTG 1 cut(s) 300
Ama87I CYCGRG 4 cut(s) 710, 1508, 2123, 2305
AoxI GGCC 3 cut(s) 1158, 2019, 2396
ApaLI GTGCAC 1 cut(s) 1895
ApeKI GCWGC 7 cut(s) 26, 300, 505, 589, 620, 2054, 2324
ApoI RAATTY 4 cut(s) 1203, 1250, 1280, 1685
ArsI GACNNNNNNTTYG 4 cut(s) 1039, 1071, 1613, 1645
Asp700I GAANNNNTTC 2 cut(s) 918, 2349
AspLEI GCGC 3 cut(s) 505, 1649, 1714
AspS9I GGNCC 5 cut(s) 1158, 1626, 2019, 2119, 2302
AsuC2I CCSGG 4 cut(s) 1509, 1510, 1874, 2454
AsuHPI GGTGA 3 cut(s) 1015, 1516, 1910
AvaI CYCGRG 4 cut(s) 710, 1508, 2123, 2305
AvaII GGWCC 3 cut(s) 1626, 2119, 2302
BaeGI GKGCMC 1 cut(s) 1899
BanI GGYRCC 1 cut(s) 2072
BanII GRGCYC 2 cut(s) 834, 1509
BarI GAAGNNNNNNTAC 2 cut(s) 1059, 1091
BauI CACGAG 1 cut(s) 1295
BbsI GAAGAC 3 cut(s) 683, 1261, 1626
Bbv12I GWGCWC 5 cut(s) 40, 364, 834, 848, 1899
BbvCI CCTCAGC 1 cut(s) 377
BbvI GCAGC 7 cut(s) 13, 287, 517, 601, 607, 2041, 2336
BccI CCATC 5 cut(s) 70, 808, 1711, 2273, 2348
BceAI ACGGC 1 cut(s) 641
BcgI CGANNNNNNTGC 4 cut(s) 2054, 2088, 2267, 2301
BciT130I CCWGG 2 cut(s) 394, 701
BclI TGATCA 1 cut(s) 1042
BcnI CCSGG 4 cut(s) 1509, 1510, 1874, 2454
BcoDI GTCTC 5 cut(s) 194, 218, 357, 1606, 1971
BfuAI ACCTGC 1 cut(s) 243
BglII AGATCT 1 cut(s) 115
BisI GCNGC 8 cut(s) 27, 301, 506, 590, 597, 621, 2055, 2325
BlpI GCTNAGC 1 cut(s) 266
BlsI GCNGC 8 cut(s) 28, 302, 507, 591, 598, 622, 2056, 2326
BmcAI AGTACT 1 cut(s) 51
Bme1390I CCNGG 6 cut(s) 394, 701, 1509, 1510, 1874, 2454
Bme18I GGWCC 3 cut(s) 1626, 2119, 2302
BmeT110I CYCGRG 4 cut(s) 710, 1508, 2123, 2305
BmgT120I GGNCC 5 cut(s) 1158, 1626, 2019, 2119, 2302
BmiI GGNNCC 8 cut(s) 315, 1159, 1627, 2074, 2104, 2121, 2304, 2379
BmrFI CCNGG 6 cut(s) 394, 701, 1509, 1510, 1874, 2454
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 9 cut(s) 608, 705, 784, 952, 1411, 1439, 1476, 1924, 2275
BmuI ACTGGG 1 cut(s) 194
BpiI GAAGAC 3 cut(s) 683, 1261, 1626
BplI GAGNNNNNCTC 2 cut(s) 1546, 1578
Bpu10I CCTNAGC 1 cut(s) 377
Bpu1102I GCTNAGC 1 cut(s) 266
BpuEI CTTGAG 4 cut(s) 104, 1474, 1943, 2076
BpuMI CCSGG 4 cut(s) 1509, 1510, 1874, 2454
Bsa29I ATCGAT 1 cut(s) 74
BsaHI GRCGYC 1 cut(s) 2209
BsaI GGTCTC 2 cut(s) 194, 357
BsaXI ACNNNNNCTCC 4 cut(s) 174, 204, 2401, 2431
Bsc4I CCNNNNNNNGG 1 cut(s) 2312
Bse118I RCCGGY 1 cut(s) 2394
Bse1I ACTGG 4 cut(s) 189, 1064, 2190, 2438
BseBI CCWGG 2 cut(s) 394, 701
BseCI ATCGAT 1 cut(s) 74
BseGI GGATG 9 cut(s) 153, 690, 696, 967, 1105, 1426, 1960, 2434, 2463
BseLI CCNNNNNNNGG 1 cut(s) 2312
BseMII CTCAG 6 cut(s) 368, 488, 932, 1822, 2082, 2123
BseNI ACTGG 4 cut(s) 189, 1064, 2190, 2438
BseRI GAGGAG 6 cut(s) 245, 627, 630, 1166, 1264, 2422
BseSI GKGCMC 1 cut(s) 1899
BseXI GCAGC 7 cut(s) 13, 287, 517, 601, 607, 2041, 2336
BsgI GTGCAG 4 cut(s) 313, 581, 608, 1133
Bsh1236I CGCG 4 cut(s) 148, 1647, 1948, 2385
Bsh1285I CGRYCG 2 cut(s) 146, 331
BshFI GGCC 3 cut(s) 1160, 2021, 2398
BshNI GGYRCC 1 cut(s) 2072
BshVI ATCGAT 1 cut(s) 74
BsiEI CGRYCG 2 cut(s) 146, 331
BsiHKAI GWGCWC 5 cut(s) 40, 364, 834, 848, 1899
BsiHKCI CYCGRG 4 cut(s) 710, 1508, 2123, 2305
BsiSI CCGG 4 cut(s) 1509, 1873, 2395, 2453
BslFI GGGAC 4 cut(s) 410, 717, 1609, 1639
BslI CCNNNNNNNGG 1 cut(s) 2312
BsmAI GTCTC 5 cut(s) 194, 218, 357, 1606, 1971
BsmBI CGTCTC 1 cut(s) 218
BsmFI GGGAC 4 cut(s) 410, 717, 1609, 1639
BsnI GGCC 3 cut(s) 1160, 2021, 2398
Bso31I GGTCTC 2 cut(s) 194, 357
BsoBI CYCGRG 4 cut(s) 710, 1508, 2123, 2305
Bsp1286I GDGCHC 6 cut(s) 40, 364, 834, 848, 1509, 1899
Bsp1407I TGTACA 1 cut(s) 1186
Bsp143I GATC 7 cut(s) 115, 150, 169, 328, 1042, 1957, 2421
Bsp1720I GCTNAGC 1 cut(s) 266
Bsp19I CCATGG 2 cut(s) 737, 1527
BspACI CCGC 6 cut(s) 146, 148, 596, 1452, 2106, 2385
BspANI GGCC 3 cut(s) 1160, 2021, 2398
BspCNI CTCAG 6 cut(s) 369, 489, 933, 1821, 2081, 2124
BspDI ATCGAT 1 cut(s) 74
BspFNI CGCG 4 cut(s) 148, 1647, 1948, 2385
BspLI GGNNCC 8 cut(s) 315, 1159, 1627, 2074, 2104, 2121, 2304, 2379
BspMI ACCTGC 1 cut(s) 243
BspPI GGATC 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 2072
BspTNI GGTCTC 2 cut(s) 194, 357
BsrFI RCCGGY 1 cut(s) 2394
BsrGI TGTACA 1 cut(s) 1186
BsrI ACTGG 4 cut(s) 189, 1064, 2190, 2438
BssAI RCCGGY 1 cut(s) 2394
BssMI GATC 7 cut(s) 115, 150, 169, 328, 1042, 1957, 2421
BssNI GRCGYC 1 cut(s) 2209
BssSI CACGAG 1 cut(s) 1295
BssT1I CCWWGG 5 cut(s) 737, 1331, 1527, 1900, 2022
Bst2BI CACGAG 1 cut(s) 1295
Bst2UI CCWGG 2 cut(s) 394, 701
Bst4CI ACNGT 9 cut(s) 42, 54, 88, 137, 366, 556, 1003, 1090, 1600
Bst6I CTCTTC 8 cut(s) 741, 924, 1014, 1230, 1527, 1856, 1862, 2150
BstACI GRCGYC 1 cut(s) 2209
BstAPI GCANNNNNTGC 2 cut(s) 300, 962
BstAUI TGTACA 1 cut(s) 1186
BstC8I GCNNGC 7 cut(s) 594, 868, 1710, 1883, 2052, 2110, 2207
BstDEI CTNAG 8 cut(s) 266, 356, 377, 497, 941, 1808, 2068, 2132
BstDSI CCRYGG 3 cut(s) 146, 737, 1527
BstEII GGTNACC 1 cut(s) 1003
BstF5I GGATG 9 cut(s) 153, 690, 696, 967, 1105, 1426, 1960, 2434, 2463
BstFNI CGCG 4 cut(s) 148, 1647, 1948, 2385
BstHHI GCGC 3 cut(s) 505, 1649, 1714
BstKTI GATC 7 cut(s) 118, 153, 172, 331, 1045, 1960, 2424
BstMAI GTCTC 5 cut(s) 194, 218, 357, 1606, 1971
BstMBI GATC 7 cut(s) 115, 150, 169, 328, 1042, 1957, 2421
BstMCI CGRYCG 2 cut(s) 146, 331
BstNI CCWGG 2 cut(s) 394, 701
BstNSI RCATGY 1 cut(s) 274
BstPI GGTNACC 1 cut(s) 1003
BstSCI CCNGG 6 cut(s) 392, 699, 1507, 1508, 1872, 2452
BstSLI GKGCMC 1 cut(s) 1899
BstUI CGCG 4 cut(s) 148, 1647, 1948, 2385
BstV1I GCAGC 7 cut(s) 13, 287, 517, 601, 607, 2041, 2336
BstV2I GAAGAC 3 cut(s) 683, 1261, 1626
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
Bsu15I ATCGAT 1 cut(s) 74
BsuRI GGCC 3 cut(s) 1160, 2021, 2398
BsuTUI ATCGAT 1 cut(s) 74
BtgI CCRYGG 3 cut(s) 146, 737, 1527
BtsCI GGATG 9 cut(s) 153, 690, 696, 967, 1105, 1426, 1960, 2434, 2463
BtsI GCAGTG 3 cut(s) 12, 586, 639
BtsIMutI CAGTG 6 cut(s) 12, 38, 182, 362, 586, 639
BveI ACCTGC 1 cut(s) 243
Cac8I GCNNGC 7 cut(s) 594, 868, 1710, 1883, 2052, 2110, 2207
CaiI CAGNNNCTG 1 cut(s) 300
CfoI GCGC 3 cut(s) 505, 1649, 1714
Cfr10I RCCGGY 1 cut(s) 2394
Cfr13I GGNCC 5 cut(s) 1158, 1626, 2019, 2119, 2302
Cfr42I CCGCGG 1 cut(s) 149
Cfr9I CCCGGG 1 cut(s) 1508
ClaI ATCGAT 1 cut(s) 74
CseI GACGC 2 cut(s) 1049, 2198
Csp6I GTAC 7 cut(s) 50, 97, 644, 1187, 1750, 1870, 2128
CviQI GTAC 7 cut(s) 50, 97, 644, 1187, 1750, 1870, 2128
DdeI CTNAG 8 cut(s) 266, 356, 377, 497, 941, 1808, 2068, 2132
DpnI GATC 7 cut(s) 117, 152, 171, 330, 1044, 1959, 2423
DpnII GATC 7 cut(s) 115, 150, 169, 328, 1042, 1957, 2421
DraI TTTAAA 1 cut(s) 691
Eam1104I CTCTTC 8 cut(s) 741, 924, 1014, 1230, 1527, 1856, 1862, 2150
EarI CTCTTC 8 cut(s) 741, 924, 1014, 1230, 1527, 1856, 1862, 2150
Ecl136II GAGCTC 1 cut(s) 832
Eco130I CCWWGG 5 cut(s) 737, 1331, 1527, 1900, 2022
Eco24I GRGCYC 2 cut(s) 834, 1509
Eco31I GGTCTC 2 cut(s) 194, 357
Eco47I GGWCC 3 cut(s) 1626, 2119, 2302
Eco53kI GAGCTC 1 cut(s) 832
Eco57I CTGAAG 1 cut(s) 1999
Eco88I CYCGRG 4 cut(s) 710, 1508, 2123, 2305
Eco91I GGTNACC 1 cut(s) 1003
EcoICRI GAGCTC 1 cut(s) 832
EcoO109I RGGNCCY 1 cut(s) 1626
EcoO65I GGTNACC 1 cut(s) 1003
EcoRI GAATTC 1 cut(s) 1685
EcoRII CCWGG 2 cut(s) 392, 699
EcoT14I CCWWGG 5 cut(s) 737, 1331, 1527, 1900, 2022
EcoT22I ATGCAT 1 cut(s) 1917
EcoT38I GRGCYC 2 cut(s) 834, 1509
ErhI CCWWGG 5 cut(s) 737, 1331, 1527, 1900, 2022
Esp3I CGTCTC 1 cut(s) 218
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 4 cut(s) 410, 717, 1609, 1639
FbaI TGATCA 1 cut(s) 1042
FblI GTMKAC 1 cut(s) 2166
Fnu4HI GCNGC 8 cut(s) 27, 301, 506, 590, 597, 621, 2055, 2325
FokI GGATG 8 cut(s) 140, 683, 697, 974, 1112, 1433, 1947, 2421
FriOI GRGCYC 2 cut(s) 834, 1509
Fsp4HI GCNGC 8 cut(s) 27, 301, 506, 590, 597, 621, 2055, 2325
GlaI GCGC 3 cut(s) 504, 1648, 1713
GluI GCNGC 8 cut(s) 27, 301, 506, 590, 597, 621, 2055, 2325
HaeIII GGCC 3 cut(s) 1160, 2021, 2398
HapII CCGG 4 cut(s) 1509, 1873, 2395, 2453
HgaI GACGC 2 cut(s) 1049, 2198
HhaI GCGC 3 cut(s) 505, 1649, 1714
Hin1I GRCGYC 1 cut(s) 2209
Hin6I GCGC 3 cut(s) 503, 1647, 1712
HinP1I GCGC 3 cut(s) 503, 1647, 1712
HindIII AAGCTT 2 cut(s) 263, 973
HpaII CCGG 4 cut(s) 1509, 1873, 2395, 2453
HphI GGTGA 3 cut(s) 1015, 1516, 1910
Hpy166II GTNNAC 8 cut(s) 126, 140, 717, 1524, 1870, 1897, 2167, 2274
Hpy188I TCNGA 9 cut(s) 313, 469, 1084, 1243, 1279, 1420, 1811, 1965, 2214
Hpy188III TCNNGA 6 cut(s) 537, 710, 1428, 1594, 2354, 2429
Hpy8I GTNNAC 8 cut(s) 126, 140, 717, 1524, 1870, 1897, 2167, 2274
Hpy99I CGWCG 1 cut(s) 1003
HpyAV CCTTC 6 cut(s) 75, 397, 664, 814, 1046, 1166
HpyCH4III ACNGT 9 cut(s) 42, 54, 88, 137, 366, 556, 1003, 1090, 1600
HpyCH4IV ACGT 4 cut(s) 177, 211, 285, 852
HpyF3I CTNAG 8 cut(s) 266, 356, 377, 497, 941, 1808, 2068, 2132
HpySE526I ACGT 4 cut(s) 177, 211, 285, 852
Hsp92I GRCGYC 1 cut(s) 2209
HspAI GCGC 3 cut(s) 503, 1647, 1712
Ksp22I TGATCA 1 cut(s) 1042
KspI CCGCGG 1 cut(s) 149
Kzo9I GATC 7 cut(s) 115, 150, 169, 328, 1042, 1957, 2421
Lsp1109I GCAGC 7 cut(s) 13, 287, 517, 601, 607, 2041, 2336
LweI GCATC 9 cut(s) 608, 705, 784, 952, 1411, 1439, 1476, 1924, 2275
MaeII ACGT 4 cut(s) 177, 211, 285, 852
MaeIII GTNAC 4 cut(s) 985, 1003, 1841, 2197
MalI GATC 7 cut(s) 117, 152, 171, 330, 1044, 1959, 2423
MboI GATC 7 cut(s) 115, 150, 169, 328, 1042, 1957, 2421
MfeI CAATTG 2 cut(s) 341, 1342
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 6 cut(s) 40, 364, 834, 848, 1509, 1899
MluI ACGCGT 1 cut(s) 1946
MlyI GAGTC 5 cut(s) 719, 722, 1247, 1608, 2060
MmeI TCCRAC 3 cut(s) 241, 447, 2016
Mph1103I ATGCAT 1 cut(s) 1917
MroXI GAANNNNTTC 2 cut(s) 918, 2349
MseI TTAA 2 cut(s) 690, 2003
MslI CAYNNNNRTG 3 cut(s) 1500, 1764, 2368
MspA1I CMGCKG 2 cut(s) 148, 2108
MspI CCGG 4 cut(s) 1509, 1873, 2395, 2453
MspR9I CCNGG 6 cut(s) 394, 701, 1509, 1510, 1874, 2454
MssI GTTTAAAC 1 cut(s) 691
MunI CAATTG 2 cut(s) 341, 1342
MvaI CCWGG 2 cut(s) 394, 701
MvnI CGCG 4 cut(s) 148, 1647, 1948, 2385
NciI CCSGG 4 cut(s) 1509, 1510, 1874, 2454
NcoI CCATGG 2 cut(s) 737, 1527
NdeII GATC 7 cut(s) 115, 150, 169, 328, 1042, 1957, 2421
NlaIV GGNNCC 8 cut(s) 315, 1159, 1627, 2074, 2104, 2121, 2304, 2379
NmeAIII GCCGAG 1 cut(s) 342
NmuCI GTSAC 1 cut(s) 1003
NsiI ATGCAT 1 cut(s) 1917
NspI RCATGY 1 cut(s) 274
PaeR7I CTCGAG 1 cut(s) 710
PcsI WCGNNNNNNNCGW 1 cut(s) 1424
PdmI GAANNNNTTC 2 cut(s) 918, 2349
PfeI GAWTC 5 cut(s) 71, 914, 980, 1699, 2350
PkrI GCNGC 8 cut(s) 28, 302, 507, 591, 598, 622, 2056, 2326
Ple19I CGATCG 1 cut(s) 331
PleI GAGTC 5 cut(s) 719, 721, 1246, 1608, 2060
PmeI GTTTAAAC 1 cut(s) 691
PpsI GAGTC 5 cut(s) 719, 721, 1246, 1608, 2060
PpuMI RGGWCCY 1 cut(s) 1626
PsiI TTATAA 1 cut(s) 1605
Psp124BI GAGCTC 1 cut(s) 834
Psp1406I AACGTT 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 1626
Psp6I CCWGG 2 cut(s) 392, 699
PspEI GGTNACC 1 cut(s) 1003
PspGI CCWGG 2 cut(s) 392, 699
PspN4I GGNNCC 8 cut(s) 315, 1159, 1627, 2074, 2104, 2121, 2304, 2379
PspPI GGNCC 5 cut(s) 1158, 1626, 2019, 2119, 2302
PspPPI RGGWCCY 1 cut(s) 1626
PsrI GAACNNNNNNTAC 4 cut(s) 819, 851, 2349, 2381
PstNI CAGNNNCTG 1 cut(s) 300
PsuI RGATCY 1 cut(s) 115
PvuI CGATCG 1 cut(s) 331
RsaI GTAC 7 cut(s) 51, 98, 645, 1188, 1751, 1871, 2129
RsaNI GTAC 7 cut(s) 50, 97, 644, 1187, 1750, 1870, 2128
RseI CAYNNNNRTG 3 cut(s) 1500, 1764, 2368
SacI GAGCTC 1 cut(s) 834
SacII CCGCGG 1 cut(s) 149
SaqAI TTAA 2 cut(s) 690, 2003
SatI GCNGC 8 cut(s) 27, 301, 506, 590, 597, 621, 2055, 2325
Sau3AI GATC 7 cut(s) 115, 150, 169, 328, 1042, 1957, 2421
Sau96I GGNCC 5 cut(s) 1158, 1626, 2019, 2119, 2302
ScaI AGTACT 1 cut(s) 51
SchI GAGTC 5 cut(s) 719, 722, 1247, 1608, 2060
ScrFI CCNGG 6 cut(s) 394, 701, 1509, 1510, 1874, 2454
SduI GDGCHC 6 cut(s) 40, 364, 834, 848, 1509, 1899
SfaNI GCATC 9 cut(s) 608, 705, 784, 952, 1411, 1439, 1476, 1924, 2275
Sfr274I CTCGAG 1 cut(s) 710
Sfr303I CCGCGG 1 cut(s) 149
SgrBI CCGCGG 1 cut(s) 149
SinI GGWCC 3 cut(s) 1626, 2119, 2302
SlaI CTCGAG 1 cut(s) 710
SmaI CCCGGG 1 cut(s) 1510
SmiMI CAYNNNNRTG 3 cut(s) 1500, 1764, 2368
SmlI CTYRAG 5 cut(s) 119, 710, 1489, 1922, 2091
SmoI CTYRAG 5 cut(s) 119, 710, 1489, 1922, 2091
SsiI CCGC 6 cut(s) 146, 148, 596, 1452, 2106, 2385
SstI GAGCTC 1 cut(s) 834
StyD4I CCNGG 6 cut(s) 392, 699, 1507, 1508, 1872, 2452
StyI CCWWGG 5 cut(s) 737, 1331, 1527, 1900, 2022
TaaI ACNGT 9 cut(s) 42, 54, 88, 137, 366, 556, 1003, 1090, 1600
TaiI ACGT 4 cut(s) 180, 214, 288, 855
TatI WGTACW 3 cut(s) 49, 96, 1186
TauI GCSGC 1 cut(s) 599
TfiI GAWTC 5 cut(s) 71, 914, 980, 1699, 2350
Tru1I TTAA 2 cut(s) 690, 2003
Tru9I TTAA 2 cut(s) 690, 2003
TscAI CASTG 6 cut(s) 12, 45, 189, 369, 586, 639
TseFI GTSAC 1 cut(s) 1003
TseI GCWGC 7 cut(s) 26, 300, 505, 589, 620, 2054, 2324
Tsp45I GTSAC 1 cut(s) 1003
TspDTI ATGAA 9 cut(s) 462, 897, 927, 1196, 1532, 1776, 1782, 1961, 2279
TspGWI ACGGA 3 cut(s) 1068, 2021, 2165
TspMI CCCGGG 1 cut(s) 1508
TspRI CASTG 6 cut(s) 12, 45, 189, 369, 586, 639
VneI GTGCAC 1 cut(s) 1895
VpaK11BI GGWCC 3 cut(s) 1626, 2119, 2302
XapI RAATTY 4 cut(s) 1203, 1250, 1280, 1685
XceI RCATGY 1 cut(s) 274
XhoI CTCGAG 1 cut(s) 710
XmaI CCCGGG 1 cut(s) 1508
XmiI GTMKAC 1 cut(s) 2166
XmnI GAANNNNTTC 2 cut(s) 918, 2349
ZrmI AGTACT 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 1917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.