pycom06g12340

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0011770
Physical Location & Seq
Forward (+)
17470693 .. 17476072
5380 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2520 bp
ATGTATAGGACACCTTCGTCCTCCAACTGCACAAACCTCCCTTCACTTTCGATCCCTCCCTCTCTAACAAAAACAAAAAACAGCAACAAGAAGAAGGTTTTTTTTTTTTTTTTTCCTGACCAAAAAAGAGAAGCTTTTGGAACCCCCCTCCTCCTCTCTCTCTCTGCAGAAATTCAAGACGAAGGCGATCTCTGTATCCTTCCAAACATGCATTTCGCAAAGCTAGATGATTCGCCTATGTTCCGCCAACAGATACAAGGCTTGGAGGAAAGTGCAGAGTCACTGAGGGGAAGATGTTATAAGTTTTACAAAGGATGCCGAAAGTATACAGAAGGGCTTGGAGAAGCGTATGATGGTGAAATCGCTTTTATAACTGCCCTTGAAAGTTTTGGGGGAGGCCACAATGATCCTATTTTTTCAGCTTTAGGAAGCCAGGTTATGTCCAGATTCACAATTGCATTAAGAGAAATCGCGACGTACAAGGAAGTTTTGCTTTCACAGATAGAGCACATGCTAAATGATAGGCTGGTGAACATTGTCCATGTTGACCTCCATGAAGTCAAGGAATCCCGAAAGCGTTTTGACAAGACTTCACTTGTATATGATCAGGCACGTGAAAAGTTTCTGTCATTGAGGAAGAGCACGAGGATGGATATAGCTGCTAGTATAGAGGAGGAACTGCAGAATGCAAGATCCTCGTTTGAGCAAGCCCGCTTCAGCCTGGTTGCTGTTCTTTCTGATATTGAGGCCAAAAAGAGGTTTGAGTTTCTGGAAGCTGTCAGCGGGGTGATGGACGCTCATCTTCGTTACTTCAAACAGAGTTATGAGTTACTGCATGCAATGGAACCCTACATTCATCAGGCTTTGGCTTACGCGCAACAGACACGAGAAAGTTGCAACCAGGAACAGACGTGTCTCAATGAAAGGATACAAGAGTACAAAAAGCAGATTGATCGGGAAAGTACGCAGTCCTTGAGCGGTATCCATGGTTCTCCGCAACCTTTCTCCAGGAAATCACATAAAGTGATCGAGGAAGTGATGCAATCTGCTGCAAAGGGGAAGGTACAAACCATTCGGCAAGGATATCTATCAAAGCGTTCCTCCAATTTGAGAGGTGACTGGAAAAGAAGGTATTTCATTCTTGATAGTCGAGGGATGCTGTACTACTATCGCAAACCATGGAGTCGGCCATCTTATGGGAGTCCATCTTCTCCACAGAAGAATAATTCCTCTGAAAACGGCTCTGGGCTTTTGAGTCGATGGCTTTCATCTCATTACCATGGTGGTATACATGATGAGAAATCCGTTGCTAGCCACACTGTGAACTTGCTGACATCAACGATAAAGGTTGATGCAGACCAGACGGACTTAAGGTTCTGCTTCAGAATTATATCACCTGCTAAAATCTACACTCTGCAGGCAGAGAATGCACTTGACCAAATGGATTGGATTGAGAAAATAACTGGAGTAATAGCTTCATTGTTGAGTTTCCAGACACTTGAGAGGCGTCTCTCAACTAGCCCTACAGGAAGCGATGATCAATTCTCTGGAAACGAGGGCTACTTATTAGAAAGTGCTCCTGATGCTGAGCAGACAGCTATTCCGGAACTGTCACCTAAGATCACAGCTTATAATTATCTACGAATCTCTAGAAGTATGCAGCCGCACAAGCTCAAAAGTGAGAAGCCAATTGACATACTGAGAAGAGTGTGCGGTAATGAAAAATGTGCTGATTGTGGTGCACCTGAACCAGATTGGGCATCTTTGAACCTCGGAATTCTTATTTGCATTGAATGCTCTGGTGTTCATCGTAATCTTGGTGTACATGTATCAAAGGTTCGATCACTAACACTTGATGTGAAAGTATGGGAACCTTCTGTCTTAACCTTGTTTCAATCACTGGGAAATGCTTTCGCAAACTCGATTTGGGAGGAGATGTTGTATTCAAGAAGTACTTTGCAGGCTGATAAAATGCCTATAGGTTTCTCCAAGGCTGATAGGAATGAGCCGTTTCTTATGATAAAGCCATGTAATGACGATCTTAATTCGACAAAGGAGAGGTATATTCAAGCAAAGTATGCAGAAAAACTTTTTGTTCGTCGTACAAAAAACAATCAGAAACTTCATGTCATGGGGCAACAGTTGTGCAAAAGCGTCCGTGCTAATGACAAGAAAGCAGTTTACCGCTATATCGTTGGTTATGAAGCAGATGTTAATTCTAGATCTGTGCAAGCATTAGATGATAATTCCGGAGACATGCCCTCCTCAAGTAACTTAAATTCCGTCACTCAAAGTGAAATTCAGCCCGCAGAAGACACCGCTGAAGGCTGTTCCCTGCTTCACCTAGCCTGCCAAAGTGCCGATATTGGCATGGTAGAGCTCCTTTTACAGTACGGTGCCAACGTAAAAGCTACTGATTCAAAAGGTCAAACACCACTACATTATTCCGTTATAAGAGGGAATATTGCTATTGCAAAGTTGCTTCTAACAAGGGGAGCTGGATCATCCAAAAGCCACTGA

Protein Analysis

840

Amino Acids

94.59

Weight (kDa)

8.55

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 75 - 304 7.3e-38 BAR domain of APPL family
BAR PF03114 144 - 285 1.2e-13 BAR domain
BAR_2 PF10455 145 - 279 8.8e-06 Bin/amphiphysin/Rvs domain for vesicular trafficking
PH PF00169 358 - 491 5.5e-11 PH domain
ArfGap PF01412 564 - 699 3.2e-31 Putative GTPase activating protein for Arf
Ank_2 PF12796 772 - 833 1.8e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 780 - 829 1.6e-09 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011770)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61980
fragaria_vesca FvH4_5g09370 FvH4_5g09370 FvH4_5g09370
malus_domestica MD06G1131800.v1.1 MD14G1148100.v1.1
prunus_persica Prupe.5G142800_v2.0.a1
pyrus_communis pycom06g12340 pycom14g12290
rosa_chinensis RchiOBHm_Chr7g0188801
rosa_laevigata RLG00000004658
rosa_multiflora Rmu_sc0003642.1_g000001
rosa_roxburghii Rroxscaffold_3G00266010
rosa_rugosa Rorug06G0494100
rosa_samantha Rh7AG098800 Rh7BG100500 Rh7CG102000 Rh7DG100600
rosa_wichuraiana Rw7G008510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 300, 371, 1632, 2453
AarI CACCTGC 1 cut(s) 1405
AasI GACNNNNNNGTC 1 cut(s) 16
Acc36I ACCTGC 1 cut(s) 1405
AccB1I GGYRCC 1 cut(s) 2396
AccB7I CCANNNNNTGG 1 cut(s) 1196
AccBSI CCGCTC 1 cut(s) 978
AccI GTMKAC 2 cut(s) 326, 1288
AccII CGCG 2 cut(s) 473, 875
AccIII TCCGGA 2 cut(s) 1603, 2249
AclWI GGATC 4 cut(s) 46, 401, 687, 2509
AcoI YGGCCR 1 cut(s) 1187
AcsI RAATTY 4 cut(s) 171, 1776, 2278, 2298
AcuI CTGAAG 3 cut(s) 700, 1366, 2343
AcvI CACGTG 1 cut(s) 614
AcyI GRCGYC 1 cut(s) 1507
AdeI CACNNNGTG 1 cut(s) 1321
AfaI GTAC 9 cut(s) 479, 938, 964, 1065, 1163, 1824, 1954, 2104, 2393
AfiI CCNNNNNNNGG 2 cut(s) 756, 1196
AflII CTTAAG 1 cut(s) 1369
AflIII ACRYGT 2 cut(s) 911, 1825
AgsI TTSAA 9 cut(s) 176, 383, 814, 1768, 1793, 1895, 1947, 2069, 2421
AjiI CACGTC 1 cut(s) 912
AjnI CCWGG 4 cut(s) 432, 720, 900, 1007
AloI GAACNNNNNNTCC 2 cut(s) 1358, 1390
Alw21I GWGCWC 5 cut(s) 510, 644, 1579, 1744, 2382
Alw26I GTCTC 3 cut(s) 920, 1514, 2247
Alw44I GTGCAC 1 cut(s) 1740
AlwI GGATC 4 cut(s) 46, 401, 687, 2509
Aor13HI TCCGGA 2 cut(s) 1603, 2249
AoxI GGCC 3 cut(s) 397, 747, 1187
ApaLI GTGCAC 1 cut(s) 1740
ApeKI GCWGC 3 cut(s) 659, 1049, 1660
ApoI RAATTY 4 cut(s) 171, 1776, 2278, 2298
Asp700I GAANNNNTTC 1 cut(s) 621
AspLEI GCGC 1 cut(s) 877
AsuHPI GGTGA 7 cut(s) 368, 541, 799, 1127, 1386, 1605, 2333
AsuNHI GCTAGC 1 cut(s) 1310
BaeGI GKGCMC 1 cut(s) 1744
BaeI ACNNNNGTAYC 2 cut(s) 920, 953
BanI GGYRCC 1 cut(s) 2396
BanII GRGCYC 1 cut(s) 2382
BauI CACGAG 2 cut(s) 643, 885
BbrPI CACGTG 1 cut(s) 614
BbsI GAAGAC 1 cut(s) 2319
Bbv12I GWGCWC 5 cut(s) 510, 644, 1579, 1744, 2382
BbvI GCAGC 3 cut(s) 646, 1036, 1672
BccI CCATC 6 cut(s) 347, 643, 784, 1198, 1213, 1254
BceAI ACGGC 2 cut(s) 1255, 1993
BcgI CGANNNNNNTGC 6 cut(s) 678, 712, 876, 910, 935, 969
BciT130I CCWGG 4 cut(s) 434, 722, 902, 1009
BciVI GTATCC 3 cut(s) 206, 921, 992
BclI TGATCA 2 cut(s) 604, 1537
BcoDI GTCTC 3 cut(s) 920, 1514, 2247
BfaI CTAG 7 cut(s) 224, 663, 1311, 1518, 1650, 2220, 2345
BfmI CTRYAG 5 cut(s) 165, 680, 1415, 1524, 1977
BfrI CTTAAG 1 cut(s) 1369
BfuAI ACCTGC 1 cut(s) 1405
BfuI GTATCC 3 cut(s) 206, 921, 992
BglII AGATCT 1 cut(s) 2222
BisI GCNGC 4 cut(s) 660, 1050, 1661, 1664
BlpI GCTNAGC 1 cut(s) 1587
BlsI GCNGC 4 cut(s) 661, 1051, 1662, 1665
BmcAI AGTACT 1 cut(s) 1954
Bme1390I CCNGG 4 cut(s) 434, 722, 902, 1009
BmgBI CACGTC 1 cut(s) 912
BmiI GGNNCC 4 cut(s) 142, 846, 1872, 2398
BmrFI CCNGG 4 cut(s) 434, 722, 902, 1009
BmrI ACTGGG 1 cut(s) 1910
BmsI GCATC 6 cut(s) 305, 1029, 1146, 1342, 1573, 1769
BmtI GCTAGC 1 cut(s) 1314
BmuI ACTGGG 1 cut(s) 1910
BpiI GAAGAC 1 cut(s) 2319
BpmI CTGGAG 2 cut(s) 991, 1485
Bpu1102I GCTNAGC 1 cut(s) 1587
BpuEI CTTGAG 3 cut(s) 994, 1520, 2251
BsaAI YACGTR 1 cut(s) 614
BsaBI GATNNNNATC 1 cut(s) 1087
BsaHI GRCGYC 1 cut(s) 1507
BsaJI CCNNGG 5 cut(s) 985, 1178, 1279, 1771, 1989
BsaWI WCCGGW 2 cut(s) 1603, 2249
BsaXI ACNNNNNCTCC 6 cut(s) 1922, 1925, 1952, 1955, 2246, 2276
Bsc4I CCNNNNNNNGG 2 cut(s) 756, 1196
Bse1I ACTGG 3 cut(s) 1124, 1468, 1905
Bse3DI GCAATG 1 cut(s) 846
Bse8I GATNNNNATC 1 cut(s) 1087
BseAI TCCGGA 2 cut(s) 1603, 2249
BseBI CCWGG 4 cut(s) 434, 722, 902, 1009
BseDI CCNNGG 5 cut(s) 985, 1178, 1279, 1771, 1989
BseGI GGATG 4 cut(s) 320, 654, 1161, 2504
BseJI GATNNNNATC 1 cut(s) 1087
BseLI CCNNNNNNNGG 2 cut(s) 756, 1196
BseMI GCAATG 1 cut(s) 846
BseMII CTCAG 3 cut(s) 275, 1578, 1691
BseNI ACTGG 3 cut(s) 1124, 1468, 1905
BseRI GAGGAG 5 cut(s) 140, 143, 686, 1946, 2254
BseSI GKGCMC 1 cut(s) 1744
BseXI GCAGC 3 cut(s) 646, 1036, 1672
BsgI GTGCAG 2 cut(s) 13, 294
Bsh1236I CGCG 2 cut(s) 473, 875
BshFI GGCC 3 cut(s) 399, 749, 1189
BshNI GGYRCC 1 cut(s) 2396
BsiHKAI GWGCWC 5 cut(s) 510, 644, 1579, 1744, 2382
BsiSI CCGG 2 cut(s) 1604, 2250
BslI CCNNNNNNNGG 2 cut(s) 756, 1196
BsmAI GTCTC 3 cut(s) 920, 1514, 2247
BsmBI CGTCTC 1 cut(s) 1514
BsmI GAATGC 3 cut(s) 691, 1432, 1799
BsnI GGCC 3 cut(s) 399, 749, 1189
Bsp1286I GDGCHC 5 cut(s) 510, 644, 1579, 1744, 2382
Bsp13I TCCGGA 2 cut(s) 1603, 2249
Bsp1407I TGTACA 1 cut(s) 1822
Bsp1720I GCTNAGC 1 cut(s) 1587
Bsp19I CCATGG 3 cut(s) 985, 1178, 1279
Bsp68I TCGCGA 1 cut(s) 473
BspANI GGCC 3 cut(s) 399, 749, 1189
BspCNI CTCAG 3 cut(s) 276, 1579, 1692
BspEI TCCGGA 2 cut(s) 1603, 2249
BspFNI CGCG 2 cut(s) 473, 875
BspLI GGNNCC 4 cut(s) 142, 846, 1872, 2398
BspMAI CTGCAG 3 cut(s) 169, 684, 1419
BspMI ACCTGC 1 cut(s) 1405
BspOI GCTAGC 1 cut(s) 1314
BspPI GGATC 4 cut(s) 46, 401, 687, 2509
BspQI GCTCTTC 1 cut(s) 632
BspT107I GGYRCC 1 cut(s) 2396
BspTI CTTAAG 1 cut(s) 1369
BsrBI CCGCTC 1 cut(s) 978
BsrDI GCAATG 1 cut(s) 846
BsrGI TGTACA 1 cut(s) 1822
BsrI ACTGG 3 cut(s) 1124, 1468, 1905
BssECI CCNNGG 5 cut(s) 985, 1178, 1279, 1771, 1989
BssNAI GTATAC 2 cut(s) 327, 1289
BssNI GRCGYC 1 cut(s) 1507
BssSI CACGAG 2 cut(s) 643, 885
BssT1I CCWWGG 4 cut(s) 985, 1178, 1279, 1989
Bst1107I GTATAC 2 cut(s) 327, 1289
Bst2BI CACGAG 2 cut(s) 643, 885
Bst2UI CCWGG 4 cut(s) 434, 722, 902, 1009
Bst4CI ACNGT 5 cut(s) 1321, 1611, 2142, 2391, 2396
Bst6I CTCTTC 2 cut(s) 632, 1699
BstACI GRCGYC 1 cut(s) 1507
BstAFI CTTAAG 1 cut(s) 1369
BstAPI GCANNNNNTGC 3 cut(s) 1427, 1794, 2078
BstAUI TGTACA 1 cut(s) 1822
BstBAI YACGTR 1 cut(s) 614
BstC8I GCNNGC 9 cut(s) 708, 712, 837, 1312, 1419, 1962, 2232, 2307, 2350
BstDEI CTNAG 4 cut(s) 284, 1587, 1617, 1700
BstDSI CCRYGG 3 cut(s) 985, 1178, 1279
BstF5I GGATG 4 cut(s) 320, 654, 1161, 2504
BstFNI CGCG 2 cut(s) 473, 875
BstHHI GCGC 1 cut(s) 877
BstMAI GTCTC 3 cut(s) 920, 1514, 2247
BstMWI GCNNNNNNNGC 5 cut(s) 1427, 1583, 1669, 1794, 2078
BstNI CCWGG 4 cut(s) 434, 722, 902, 1009
BstNSI RCATGY 5 cut(s) 211, 514, 839, 1829, 2260
BstSCI CCNGG 4 cut(s) 432, 720, 900, 1007
BstSFI CTRYAG 5 cut(s) 165, 680, 1415, 1524, 1977
BstSLI GKGCMC 1 cut(s) 1744
BstUI CGCG 2 cut(s) 473, 875
BstV1I GCAGC 3 cut(s) 646, 1036, 1672
BstV2I GAAGAC 1 cut(s) 2319
BstX2I RGATCY 2 cut(s) 692, 2222
BstYI RGATCY 2 cut(s) 692, 2222
BstZ17I GTATAC 2 cut(s) 327, 1289
BsuI GTATCC 3 cut(s) 206, 921, 992
BsuRI GGCC 3 cut(s) 399, 749, 1189
BtgI CCRYGG 3 cut(s) 985, 1178, 1279
BtgZI GCGATG 1 cut(s) 1548
BtrI CACGTC 1 cut(s) 912
BtsCI GGATG 4 cut(s) 320, 654, 1161, 2504
BtsIMutI CAGTG 4 cut(s) 281, 1317, 1898, 2515
BtuMI TCGCGA 1 cut(s) 473
BveI ACCTGC 1 cut(s) 1405
Cac8I GCNNGC 9 cut(s) 708, 712, 837, 1312, 1419, 1962, 2232, 2307, 2350
CfoI GCGC 1 cut(s) 877
CseI GACGC 3 cut(s) 803, 1496, 2143
Csp6I GTAC 9 cut(s) 478, 937, 963, 1064, 1162, 1823, 1953, 2103, 2392
CviQI GTAC 9 cut(s) 478, 937, 963, 1064, 1162, 1823, 1953, 2103, 2392
DdeI CTNAG 4 cut(s) 284, 1587, 1617, 1700
DraIII CACNNNGTG 1 cut(s) 1321
DrdI GACNNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 16
EaeI YGGCCR 1 cut(s) 1187
Eam1104I CTCTTC 2 cut(s) 632, 1699
EarI CTCTTC 2 cut(s) 632, 1699
EciI GGCGGA 1 cut(s) 233
Ecl136II GAGCTC 1 cut(s) 2380
Eco130I CCWWGG 4 cut(s) 985, 1178, 1279, 1989
Eco24I GRGCYC 1 cut(s) 2382
Eco32I GATATC 1 cut(s) 1085
Eco53kI GAGCTC 1 cut(s) 2380
Eco57I CTGAAG 3 cut(s) 700, 1366, 2343
Eco72I CACGTG 1 cut(s) 614
EcoICRI GAGCTC 1 cut(s) 2380
EcoRI GAATTC 1 cut(s) 1776
EcoRII CCWGG 4 cut(s) 432, 720, 900, 1007
EcoRV GATATC 1 cut(s) 1085
EcoT14I CCWWGG 4 cut(s) 985, 1178, 1279, 1989
EcoT22I ATGCAT 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 2382
ErhI CCWWGG 4 cut(s) 985, 1178, 1279, 1989
Esp3I CGTCTC 1 cut(s) 1514
FalI AAGNNNNNCTT 6 cut(s) 118, 150, 477, 509, 1939, 1971
FauI CCCGC 3 cut(s) 719, 776, 2314
FbaI TGATCA 2 cut(s) 604, 1537
FblI GTMKAC 2 cut(s) 326, 1288
Fnu4HI GCNGC 4 cut(s) 660, 1050, 1661, 1664
FokI GGATG 4 cut(s) 327, 661, 1168, 2491
FriOI GRGCYC 1 cut(s) 2382
Fsp4HI GCNGC 4 cut(s) 660, 1050, 1661, 1664
FspBI CTAG 7 cut(s) 224, 663, 1311, 1518, 1650, 2220, 2345
GlaI GCGC 1 cut(s) 876
GluI GCNGC 4 cut(s) 660, 1050, 1661, 1664
GsuI CTGGAG 2 cut(s) 991, 1485
HaeIII GGCC 3 cut(s) 399, 749, 1189
HapII CCGG 2 cut(s) 1604, 2250
HgaI GACGC 3 cut(s) 803, 1496, 2143
HhaI GCGC 1 cut(s) 877
Hin1I GRCGYC 1 cut(s) 1507
Hin6I GCGC 1 cut(s) 875
HinP1I GCGC 1 cut(s) 875
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HindIII AAGCTT 1 cut(s) 132
HinfI GANTC 9 cut(s) 230, 278, 447, 566, 1183, 1201, 1255, 1644, 2417
HpaII CCGG 2 cut(s) 1604, 2250
HphI GGTGA 7 cut(s) 368, 541, 799, 1127, 1386, 1605, 2333
Hpy166II GTNNAC 8 cut(s) 327, 532, 547, 1289, 1324, 1742, 1823, 2182
Hpy188I TCNGA 5 cut(s) 739, 1234, 1385, 1775, 2118
Hpy8I GTNNAC 8 cut(s) 327, 532, 547, 1289, 1324, 1742, 1823, 2182
Hpy99I CGWCG 2 cut(s) 478, 2103
HpyCH4III ACNGT 5 cut(s) 1321, 1611, 2142, 2391, 2396
HpyCH4IV ACGT 4 cut(s) 476, 613, 911, 2403
HpyF10VI GCNNNNNNNGC 5 cut(s) 1427, 1583, 1669, 1794, 2078
HpyF3I CTNAG 4 cut(s) 284, 1587, 1617, 1700
HpySE526I ACGT 4 cut(s) 476, 613, 911, 2403
Hsp92I GRCGYC 1 cut(s) 1507
HspAI GCGC 1 cut(s) 875
Kpn2I TCCGGA 2 cut(s) 1603, 2249
Ksp22I TGATCA 2 cut(s) 604, 1537
LguI GCTCTTC 1 cut(s) 632
LmnI GCTCC 3 cut(s) 1582, 2385, 2495
Lsp1109I GCAGC 3 cut(s) 646, 1036, 1672
LweI GCATC 6 cut(s) 305, 1029, 1146, 1342, 1573, 1769
MaeI CTAG 7 cut(s) 224, 663, 1311, 1518, 1650, 2220, 2345
MaeII ACGT 4 cut(s) 476, 613, 911, 2403
MaeIII GTNAC 7 cut(s) 279, 806, 828, 1115, 1611, 2270, 2284
MbiI CCGCTC 1 cut(s) 978
MboII GAAGA 8 cut(s) 103, 303, 649, 794, 1200, 1231, 1716, 2324
MfeI CAATTG 2 cut(s) 453, 1689
MflI RGATCY 2 cut(s) 692, 2222
MhlI GDGCHC 5 cut(s) 510, 644, 1579, 1744, 2382
MlyI GAGTC 4 cut(s) 287, 1192, 1210, 1264
MmeI TCCRAC 1 cut(s) 48
Mph1103I ATGCAT 1 cut(s) 213
MroI TCCGGA 2 cut(s) 1603, 2249
MroXI GAANNNNTTC 1 cut(s) 621
MseI TTAA 6 cut(s) 461, 1370, 1883, 2043, 2214, 2276
MslI CAYNNNNRTG 2 cut(s) 647, 1278
MspA1I CMGCKG 2 cut(s) 783, 2321
MspCI CTTAAG 1 cut(s) 1369
MspI CCGG 2 cut(s) 1604, 2250
MspR9I CCNGG 4 cut(s) 434, 722, 902, 1009
MunI CAATTG 2 cut(s) 453, 1689
Mva1269I GAATGC 3 cut(s) 691, 1432, 1799
MvaI CCWGG 4 cut(s) 434, 722, 902, 1009
MvnI CGCG 2 cut(s) 473, 875
MwoI GCNNNNNNNGC 5 cut(s) 1427, 1583, 1669, 1794, 2078
NcoI CCATGG 3 cut(s) 985, 1178, 1279
NheI GCTAGC 1 cut(s) 1310
NlaIV GGNNCC 4 cut(s) 142, 846, 1872, 2398
NmuCI GTSAC 4 cut(s) 279, 1115, 1611, 2284
NruI TCGCGA 1 cut(s) 473
NsiI ATGCAT 1 cut(s) 213
NspI RCATGY 5 cut(s) 211, 514, 839, 1829, 2260
PaeI GCATGC 1 cut(s) 839
PaqCI CACCTGC 1 cut(s) 1405
PciI ACATGT 1 cut(s) 1825
PciSI GCTCTTC 1 cut(s) 632
PcsI WCGNNNNNNNCGW 1 cut(s) 2400
PctI GAATGC 3 cut(s) 691, 1432, 1799
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 5 cut(s) 230, 447, 566, 1644, 2417
PflMI CCANNNNNTGG 1 cut(s) 1196
PfoI TCCNGGA 1 cut(s) 1007
PkrI GCNGC 4 cut(s) 661, 1051, 1662, 1665
PleI GAGTC 4 cut(s) 286, 1191, 1209, 1263
PmaCI CACGTG 1 cut(s) 614
PmlI CACGTG 1 cut(s) 614
PpsI GAGTC 4 cut(s) 286, 1191, 1209, 1263
Ppu21I YACGTR 1 cut(s) 614
PscI ACATGT 1 cut(s) 1825
PsiI TTATAA 4 cut(s) 300, 371, 1632, 2453
Psp124BI GAGCTC 1 cut(s) 2382
Psp6I CCWGG 4 cut(s) 432, 720, 900, 1007
PspCI CACGTG 1 cut(s) 614
PspGI CCWGG 4 cut(s) 432, 720, 900, 1007
PspN4I GGNNCC 4 cut(s) 142, 846, 1872, 2398
PsrI GAACNNNNNNTAC 4 cut(s) 973, 1005, 1821, 1853
PstI CTGCAG 3 cut(s) 169, 684, 1419
PsuI RGATCY 2 cut(s) 692, 2222
RruI TCGCGA 1 cut(s) 473
RsaI GTAC 9 cut(s) 479, 938, 964, 1065, 1163, 1824, 1954, 2104, 2393
RsaNI GTAC 9 cut(s) 478, 937, 963, 1064, 1162, 1823, 1953, 2103, 2392
RseI CAYNNNNRTG 2 cut(s) 647, 1278
SacI GAGCTC 1 cut(s) 2382
SapI GCTCTTC 1 cut(s) 632
SaqAI TTAA 6 cut(s) 461, 1370, 1883, 2043, 2214, 2276
SatI GCNGC 4 cut(s) 660, 1050, 1661, 1664
ScaI AGTACT 1 cut(s) 1954
SchI GAGTC 4 cut(s) 287, 1192, 1210, 1264
ScrFI CCNGG 4 cut(s) 434, 722, 902, 1009
SduI GDGCHC 5 cut(s) 510, 644, 1579, 1744, 2382
SfaNI GCATC 6 cut(s) 305, 1029, 1146, 1342, 1573, 1769
SfcI CTRYAG 5 cut(s) 165, 680, 1415, 1524, 1977
SmiMI CAYNNNNRTG 2 cut(s) 647, 1278
SmlI CTYRAG 4 cut(s) 973, 1369, 1499, 2266
SmoI CTYRAG 4 cut(s) 973, 1369, 1499, 2266
SphI GCATGC 1 cut(s) 839
SspI AATATT 1 cut(s) 2464
SspMI CTAG 7 cut(s) 224, 663, 1311, 1518, 1650, 2220, 2345
SstI GAGCTC 1 cut(s) 2382
StyD4I CCNGG 4 cut(s) 432, 720, 900, 1007
StyI CCWWGG 4 cut(s) 985, 1178, 1279, 1989
TaaI ACNGT 5 cut(s) 1321, 1611, 2142, 2391, 2396
TaiI ACGT 4 cut(s) 479, 616, 914, 2406
TaqI TCGA 7 cut(s) 50, 1029, 1150, 1258, 1840, 1922, 2048
TatI WGTACW 4 cut(s) 936, 1161, 1822, 1952
TauI GCSGC 1 cut(s) 1666
TfiI GAWTC 5 cut(s) 230, 447, 566, 1644, 2417
Tru1I TTAA 6 cut(s) 461, 1370, 1883, 2043, 2214, 2276
Tru9I TTAA 6 cut(s) 461, 1370, 1883, 2043, 2214, 2276
TscAI CASTG 3 cut(s) 288, 1324, 1905
TseFI GTSAC 4 cut(s) 279, 1115, 1611, 2284
TseI GCWGC 3 cut(s) 659, 1049, 1660
Tsp45I GTSAC 4 cut(s) 279, 1115, 1611, 2284
TspGWI ACGGA 5 cut(s) 1294, 1379, 2147, 2272, 2437
TspRI CASTG 3 cut(s) 288, 1324, 1905
Van91I CCANNNNNTGG 1 cut(s) 1196
Vha464I CTTAAG 1 cut(s) 1369
VneI GTGCAC 1 cut(s) 1740
XapI RAATTY 4 cut(s) 171, 1776, 2278, 2298
XbaI TCTAGA 2 cut(s) 1649, 2219
XceI RCATGY 5 cut(s) 211, 514, 839, 1829, 2260
XmiI GTMKAC 2 cut(s) 326, 1288
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 7 cut(s) 224, 663, 1311, 1518, 1650, 2220, 2345
ZrmI AGTACT 1 cut(s) 1954
Zsp2I ATGCAT 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.