pycom06g12960

Alpha-1,4-glucan-protein synthase (UDP-forming)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014935
Physical Location & Seq
Forward (+)
18069554 .. 18070235
682 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
ATGGAGCAGCTGCGTGATCTGAAAGATGACCTAGACATTGTGATCCCAACCATCAGAAACCTTGATTTCTTGGAGATGTGGAGGCCTTTCTTCCAGCCCTACCACCTCATCATCGTCCAAGATGGCGACCCCTCCAACACCATCACCGTCCCTGATGGCTTCGACCATGAGCTCTACAACCGCAATGATGTGAACAAGGTCCTAGGTCCCAAGGCTACCTGCATTTCTTTCAAGGACTCCACTTGTCGTTGCTTCGGCTTCTTGGTTTCCAAGAAAAAGCACATCTTCACCATCGATGACGATTGCTTTGGAAAAAAATTAAAGGTGGCGAAGAAGCCTAGTGGGAAGCAGATAAATGCTTTGGCACAGCACATTAAGAACCTGCTGACACCTTCAACTCCATTTTTCTTCAACGCTCTCTACGACCCGTTTGCAAATGGGGCGGACTTTGTTCACGGGTATCCATTTAGCTTAAGAGAAGGTGTGCCCACTGCTATTTCTCACGGCCTTTGGCTCAATATCCCTGACTATGATGCCCCAACTCAGCTAGTCAAGCCTCTTGAGCGCAACACCAGATTACCATAA

Protein Analysis

195

Amino Acids

22.12

Weight (kDa)

7.0

Isoelectric Point (pI)

32.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RGP PF03214 8 - 192 8.2e-90 Reversibly glycosylated polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014935)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50750
fragaria_vesca FvH4_5g08630 FvH4_5g08630
malus_domestica MD14G1154400.v1.1
prunus_persica Prupe.5G148600_v2.0.a1
pyrus_communis pycom06g12960
rosa_chinensis RchiOBHm_Chr7g0189771
rosa_laevigata RLG00000004592
rosa_multiflora Rmu_sc0014337.1_g000001 Rmu_sc0029387.1_g000001
rosa_roxburghii Rroxscaffold_3G00265180
rosa_rugosa Rorug06G0499800 Rorug06G0499800
rosa_samantha Rh7AG104800 Rh7CG109400
rosa_wichuraiana Rw7G009070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 227, 390
AciI CCGC 2 cut(s) 181, 443
AclWI GGATC 1 cut(s) 37
AflII CTTAAG 1 cut(s) 472
AgsI TTSAA 3 cut(s) 232, 396, 412
AluBI AGCT 4 cut(s) 10, 172, 471, 547
AluI AGCT 4 cut(s) 10, 172, 471, 547
Alw21I GWGCWC 1 cut(s) 174
AlwI GGATC 1 cut(s) 37
AoxI GGCC 2 cut(s) 83, 505
ApeKI GCWGC 2 cut(s) 7, 10
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
AspA2I CCTAGG 1 cut(s) 202
AspLEI GCGC 1 cut(s) 567
AspS9I GGNCC 2 cut(s) 199, 206
AsuHPI GGTGA 2 cut(s) 136, 280
AvaII GGWCC 2 cut(s) 199, 206
AvrII CCTAGG 1 cut(s) 202
BaeGI GKGCMC 1 cut(s) 489
BanII GRGCYC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 19
BccI CCATC 5 cut(s) 59, 116, 149, 149, 299
BceAI ACGGC 1 cut(s) 520
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciVI GTATCC 1 cut(s) 471
BfaI CTAG 4 cut(s) 32, 203, 339, 548
BfrI CTTAAG 1 cut(s) 472
BfuAI ACCTGC 2 cut(s) 227, 390
BfuI GTATCC 1 cut(s) 471
BisI GCNGC 2 cut(s) 8, 11
BlnI CCTAGG 1 cut(s) 202
BlsI GCNGC 2 cut(s) 9, 12
Bme18I GGWCC 2 cut(s) 199, 206
BmgT120I GGNCC 2 cut(s) 199, 206
BmiI GGNNCC 1 cut(s) 208
BmsI GCATC 1 cut(s) 523
BpuEI CTTGAG 1 cut(s) 581
Bsa29I ATCGAT 1 cut(s) 294
BsaJI CCNNGG 2 cut(s) 202, 210
Bse3DI GCAATG 1 cut(s) 190
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 202, 210
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 557
BseSI GKGCMC 1 cut(s) 489
BseXI GCAGC 1 cut(s) 19
BshFI GGCC 2 cut(s) 85, 507
BshVI ATCGAT 1 cut(s) 294
BsiHKAI GWGCWC 1 cut(s) 174
BslFI GGGAC 2 cut(s) 134, 192
BsmFI GGGAC 2 cut(s) 134, 192
BsnI GGCC 2 cut(s) 85, 507
Bsp1286I GDGCHC 2 cut(s) 174, 489
Bsp143I GATC 2 cut(s) 16, 42
BspACI CCGC 2 cut(s) 181, 443
BspANI GGCC 2 cut(s) 85, 507
BspCNI CTCAG 1 cut(s) 556
BspDI ATCGAT 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 208
BspMI ACCTGC 2 cut(s) 227, 390
BspPI GGATC 1 cut(s) 37
BspTI CTTAAG 1 cut(s) 472
BsrDI GCAATG 1 cut(s) 190
BssECI CCNNGG 2 cut(s) 202, 210
BssMI GATC 2 cut(s) 16, 42
BssT1I CCWWGG 2 cut(s) 202, 210
Bst4CI ACNGT 1 cut(s) 148
BstAFI CTTAAG 1 cut(s) 472
BstDEI CTNAG 1 cut(s) 543
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 2 cut(s) 19, 45
BstMBI GATC 2 cut(s) 16, 42
BstMWI GCNNNNNNNGC 3 cut(s) 440, 553, 562
BstSLI GKGCMC 1 cut(s) 489
BstV1I GCAGC 1 cut(s) 19
Bsu15I ATCGAT 1 cut(s) 294
BsuI GTATCC 1 cut(s) 471
BsuRI GGCC 2 cut(s) 85, 507
BsuTUI ATCGAT 1 cut(s) 294
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 1 cut(s) 489
BveI ACCTGC 2 cut(s) 227, 390
CfoI GCGC 1 cut(s) 567
Cfr13I GGNCC 2 cut(s) 199, 206
ClaI ATCGAT 1 cut(s) 294
CviAII CATG 1 cut(s) 167
DdeI CTNAG 1 cut(s) 543
DpnI GATC 2 cut(s) 18, 44
DpnII GATC 2 cut(s) 16, 42
EciI GGCGGA 1 cut(s) 458
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 202, 210
Eco147I AGGCCT 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 174
Eco47I GGWCC 2 cut(s) 199, 206
Eco53kI GAGCTC 1 cut(s) 172
EcoICRI GAGCTC 1 cut(s) 172
EcoO109I RGGNCCY 2 cut(s) 199, 206
EcoT14I CCWWGG 2 cut(s) 202, 210
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 2 cut(s) 202, 210
FaeI CATG 1 cut(s) 170
FaiI YATR 3 cut(s) 168, 531, 583
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 2 cut(s) 134, 192
FatI CATG 1 cut(s) 166
Fnu4HI GCNGC 2 cut(s) 8, 11
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 2 cut(s) 8, 11
FspBI CTAG 4 cut(s) 32, 203, 339, 548
GlaI GCGC 1 cut(s) 566
GluI GCNGC 2 cut(s) 8, 11
HaeIII GGCC 2 cut(s) 85, 507
HhaI GCGC 1 cut(s) 567
Hin1II CATG 1 cut(s) 170
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HinfI GANTC 1 cut(s) 236
HphI GGTGA 2 cut(s) 136, 280
Hpy166II GTNNAC 2 cut(s) 193, 454
Hpy188I TCNGA 2 cut(s) 21, 56
Hpy188III TCNNGA 1 cut(s) 560
Hpy8I GTNNAC 2 cut(s) 193, 454
HpyAV CCTTC 2 cut(s) 402, 473
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4V TGCA 2 cut(s) 222, 434
HpyF10VI GCNNNNNNNGC 3 cut(s) 440, 553, 562
HpyF3I CTNAG 1 cut(s) 543
Hsp92II CATG 1 cut(s) 170
HspAI GCGC 1 cut(s) 565
Kzo9I GATC 2 cut(s) 16, 42
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 5 cut(s) 107, 165, 232, 395, 537
Lsp1109I GCAGC 1 cut(s) 19
LweI GCATC 1 cut(s) 523
MaeI CTAG 4 cut(s) 32, 203, 339, 548
MalI GATC 2 cut(s) 18, 44
MboI GATC 2 cut(s) 16, 42
MboII GAAGA 4 cut(s) 82, 277, 343, 400
MhlI GDGCHC 2 cut(s) 174, 489
MluCI AATT 1 cut(s) 317
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 4 cut(s) 75, 116, 142, 567
MseI TTAA 3 cut(s) 320, 375, 473
MspA1I CMGCKG 1 cut(s) 10
MspCI CTTAAG 1 cut(s) 472
MwoI GCNNNNNNNGC 3 cut(s) 440, 553, 562
NdeII GATC 2 cut(s) 16, 42
NlaIII CATG 1 cut(s) 170
NlaIV GGNNCC 1 cut(s) 208
PceI AGGCCT 1 cut(s) 85
PcsI WCGNNNNNNNCGW 1 cut(s) 420
PkrI GCNGC 2 cut(s) 9, 12
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
PpuMI RGGWCCY 2 cut(s) 199, 206
Psp124BI GAGCTC 1 cut(s) 174
Psp5II RGGWCCY 2 cut(s) 199, 206
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 199, 206
PspPPI RGGWCCY 2 cut(s) 199, 206
PvuII CAGCTG 1 cut(s) 10
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 3 cut(s) 320, 375, 473
SatI GCNGC 2 cut(s) 8, 11
Sau3AI GATC 2 cut(s) 16, 42
Sau96I GGNCC 2 cut(s) 199, 206
SchI GAGTC 1 cut(s) 230
SduI GDGCHC 2 cut(s) 174, 489
SfaNI GCATC 1 cut(s) 523
SinI GGWCC 2 cut(s) 199, 206
SmlI CTYRAG 2 cut(s) 472, 560
SmoI CTYRAG 2 cut(s) 472, 560
Sse9I AATT 1 cut(s) 317
SseBI AGGCCT 1 cut(s) 85
SsiI CCGC 2 cut(s) 181, 443
SspMI CTAG 4 cut(s) 32, 203, 339, 548
SstI GAGCTC 1 cut(s) 174
StuI AGGCCT 1 cut(s) 85
StyI CCWWGG 2 cut(s) 202, 210
TaaI ACNGT 1 cut(s) 148
TaqI TCGA 2 cut(s) 162, 294
TasI AATT 1 cut(s) 317
Tru1I TTAA 3 cut(s) 320, 375, 473
Tru9I TTAA 3 cut(s) 320, 375, 473
TscAI CASTG 1 cut(s) 496
TseI GCWGC 2 cut(s) 7, 10
TspRI CASTG 1 cut(s) 496
Vha464I CTTAAG 1 cut(s) 472
VpaK11BI GGWCC 2 cut(s) 199, 206
XmaJI CCTAGG 1 cut(s) 202
XspI CTAG 4 cut(s) 32, 203, 339, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.