pycom06g13430
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005997
Physical Location & Seq
Reverse (-)
18430850 .. 18433686
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2232 bp
ATGGGGCTTTCTGAATCGTTTCTGCTATTCTGTTTTTCTTGGAGCTTCTTACTTCTAGGCACTCATCAACTCCAATCTTCTCAAACCCAAGTGCTTCTGCAGCTCAGGAAGCACTTAGAGTACCCAAAACAGCTAGAAATTTGGAACAGTCACAACACAGATTTCTGCTCCATTTCTTCACTTGGCCAAGTAAACATTACATGCTTGGACAATCTTGTAATTGTGCTCAGAATCAAGGGTGATAAGATTAAGCGATCCAACAAAAGTGATTACTTCAATGGCTTTTCAATCCAAAGCCAAACTTTATCAGATTCCTTTTCTTTGGATTCTTTTGTCACTACCCTTTCAAGGCTAAACAGTTTGAGAGTTCTTAGCCTTGTGTCTTTGGGCATCTGGGGTCCACTTCCAGACAAAGTTCATAGGCTGTCTTCCCTTGAGTATCTCGATTTGAGCTCGAACTTCCTCTTCGGTTCAATCCCACCAAGGATTTCCGCAATGGCGATGCTTCAAACACTTAAAATCGATGAGAATTTCTTAAATGATACTATCCCTGATTGGTTCGATTCGCTATCCAATCTTGCAACTCTGAGCTTGAGGAACAACAAGTTGGAAGGTGCATTGCAGGATTTTAGTAGGTTAAGCAGCTTACAAGTGCTAGATTTGAGTGGGAACAGATTGAGCTCTACGTTGCCTCCAATGCCAAAAGGGTTGGTCATGCTTTTCGTCAGCGAAAACTCCTTCTCCGGCAAGATTCCAGTGAAATATGGCAAGTTAAGTGGACTTCAACACATTGATATGTCACACAATGCACTTATAGGCACACCTCCTGCTGCACTCTTCTCTTTGCCTAATATTAGTTACTTGAACTTGGGGTCAAATCTGTTGAGTGGCTCGCTTCCAGGGAATCTAATCTGTGGAAGCAAACTCGATTTCATTGATATATCGAATAATAGTTTGAGGGGTGATTTGCCTTCATGTTTAAGAAATGAATCAAAAGAGAGAGTTGTGAAGTTTGGTGGGAATTGTTTGTCAACTGGTGTGCAGTATCAGCATGAACAATCATACTGCAAGGAAGTGATCAGCCCAAGGAAGAAAGAGTTCGGAGGGAACAATGTAGGGATCTTGGTGGGTGTGATTCTAGGATTAATTGTACTTAGTGTGCTTCTGGTTTTAGGGTTTATCATTTTCTGCAGAAGATATTGCCCTCAGGGGATATCGAAGCAGCATTTGCTCCATAAATCAGTGCAAGAGAATTCAGCAGTTGGGTTTTCGACCGAGCTTCTAACAAGTCCAAGGTTTATTTCACAAGCTGGAAAGGTAGAGATACAAGGCCTCCCTGTGTGCCGGATATTTTCTATGGAAGAGCTGATGGAAGCTACGAAAAACTTCGACAGCTCTGCCTTATTGGGTGAAGGTTCATATGGGAAGCTATACAAAGGAAGACTATACAATGGGACTGAAGTTGCCATAAGATGCCTGCCTTTGTCGAAGAAGTACACAATTCGAAACGTTAAACTCCGGTTGGATCTGCTTTCCAAGCTTCGGCACCAGCATTTGGTATGCCTTTTAGGGCACTGTCTCGATGGTGGTGGACAAGATGATTACAATCCGAAAAAGGTTTATTTTGTGTCTGAATATGTGCCCAGTGGGACATTCCGGGATCATCTCTCTGGGAATAGTCCTGGAAAGGTTCTCAACTGGTCAGAGAGGTTGGCAATTCTAGTTAGCGTGGCTAAGGCCGTGCACTTCCTACATACCGGAATCATTCCTGGTTTCTTCTGCAACCGCCTGAAGACGAACAATATCTTGCTCAATGAGCACGGTATGGCAAAATTGAGTGACTATGGACTGTCCATTCTCACAGAAGAAACCGATAAATCCACAGCAAAAGACGGCCTTACATCATGGCAAATGACAAGTTTAGACGATGATGCTTATAGCTTCGGATACATACTACTCGAGGCTCTCGTTGGAACTTCCATATCTTCTACAAGAGAAGCAATCCTGCAAAATAATATGGCATCTCTGAGTAGCGAGGACAGGAGGAAGCAGATAGTTGAACCGGCTGTTCTAGCCAGTTGCTCACAAGAGTCTCTATCGATCGTGATCTCCGTAATGTACAAATGCATTTGTCCAGAGTCGAGCCGTCCATCTTTCGAGGACATCCTTTGGACCTTACAGTATGCAGTTCAAGTCCAGGAAGAAACAGCAGCTGGAGACCAAAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

744

Amino Acids

82.24

Weight (kDa)

8.02

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 112 - 223 2e-09 Leucine-rich repeat region
LRR_8 PF13855 169 - 226 2.9e-11 Leucine rich repeat
LRR_8 PF13855 260 - 319 4.9e-06 Leucine rich repeat
Pkinase PF00069 466 - 723 2.5e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 467 - 724 1.7e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1545
AccB7I CCANNNNNTGG 1 cut(s) 1555
AciI CCGC 2 cut(s) 492, 1786
AclI AACGTT 1 cut(s) 1509
AclWI GGATC 4 cut(s) 249, 1127, 1533, 1668
AcoI YGGCCR 1 cut(s) 184
AcsI RAATTY 3 cut(s) 138, 529, 1252
AcuI CTGAAG 2 cut(s) 1479, 1811
AfaI GTAC 4 cut(s) 122, 1152, 1496, 2120
AfiI CCNNNNNNNGG 3 cut(s) 348, 1542, 1555
AgsI TTSAA 9 cut(s) 277, 288, 348, 474, 509, 785, 865, 2060, 2192
AjnI CCWGG 4 cut(s) 898, 1681, 1768, 2196
AjuI GAANNNNNNNTTGG 4 cut(s) 590, 622, 1285, 1317
Alw21I GWGCWC 5 cut(s) 228, 455, 683, 1746, 1821
Alw26I GTCTC 3 cut(s) 1583, 2097, 2211
Alw44I GTGCAC 1 cut(s) 1742
AlwI GGATC 4 cut(s) 249, 1127, 1533, 1668
AlwNI CAGNNNCTG 1 cut(s) 2213
Ama87I CYCGRG 1 cut(s) 1958
AoxI GGCC 4 cut(s) 184, 1330, 1737, 1894
ApaLI GTGCAC 1 cut(s) 1742
ApeKI GCWGC 5 cut(s) 100, 642, 830, 1222, 2210
ApoI RAATTY 3 cut(s) 138, 529, 1252
AseI ATTAAT 1 cut(s) 1145
Asp700I GAANNNNTTC 3 cut(s) 18, 1097, 1385
AspS9I GGNCC 2 cut(s) 398, 2172
AsuC2I CCSGG 1 cut(s) 1658
AsuHPI GGTGA 3 cut(s) 251, 974, 1421
AsuII TTCGAA 1 cut(s) 1504
AvaI CYCGRG 1 cut(s) 1958
AvaII GGWCC 2 cut(s) 398, 2172
AxyI CCTNAGG 1 cut(s) 1206
BaeGI GKGCMC 3 cut(s) 1575, 1644, 1746
BalI TGGCCA 1 cut(s) 186
BanI GGYRCC 1 cut(s) 1545
BanII GRGCYC 2 cut(s) 455, 683
BbsI GAAGAC 3 cut(s) 420, 1447, 1799
Bbv12I GWGCWC 5 cut(s) 228, 455, 683, 1746, 1821
BbvI GCAGC 5 cut(s) 112, 654, 817, 1234, 2222
BccI CCATC 3 cut(s) 1363, 1577, 2158
BceAI ACGGC 3 cut(s) 1724, 1909, 2130
BcgI CGANNNNNNTGC 2 cut(s) 1379, 1413
BciT130I CCWGG 4 cut(s) 900, 1683, 1770, 2198
BciVI GTATCC 1 cut(s) 1940
BclI TGATCA 1 cut(s) 1077
BcnI CCSGG 1 cut(s) 1658
BcoDI GTCTC 3 cut(s) 1583, 2097, 2211
BfaI CTAG 6 cut(s) 56, 134, 656, 1139, 1721, 2072
BfmI CTRYAG 2 cut(s) 98, 1189
BfuI GTATCC 1 cut(s) 1940
BisI GCNGC 5 cut(s) 101, 643, 831, 1223, 2211
BlsI GCNGC 5 cut(s) 102, 644, 832, 1224, 2212
Bme1390I CCNGG 5 cut(s) 900, 1658, 1683, 1770, 2198
Bme18I GGWCC 2 cut(s) 398, 2172
BmeT110I CYCGRG 1 cut(s) 1958
BmgT120I GGNCC 2 cut(s) 398, 2172
BmiI GGNNCC 2 cut(s) 399, 1547
BmrFI CCNGG 5 cut(s) 900, 1658, 1683, 1770, 2198
BmrI ACTGGG 1 cut(s) 1638
BmsI GCATC 5 cut(s) 399, 492, 1463, 1921, 2030
BmuI ACTGGG 1 cut(s) 1638
BpiI GAAGAC 3 cut(s) 420, 1447, 1799
Bpu10I CCTNAGC 2 cut(s) 104, 1734
Bpu14I TTCGAA 1 cut(s) 1504
BpuEI CTTGAG 2 cut(s) 455, 613
BpuMI CCSGG 1 cut(s) 1658
Bsa29I ATCGAT 2 cut(s) 522, 2099
BsaBI GATNNNNATC 2 cut(s) 1605, 2105
BsaI GGTCTC 1 cut(s) 2211
BsaJI CCNNGG 4 cut(s) 482, 899, 1085, 1292
BsaWI WCCGGW 2 cut(s) 1518, 1757
BsaXI ACNNNNNCTCC 6 cut(s) 676, 706, 725, 755, 1317, 1347
Bsc4I CCNNNNNNNGG 3 cut(s) 348, 1542, 1555
Bse118I RCCGGY 1 cut(s) 2062
Bse1I ACTGG 5 cut(s) 755, 1039, 1644, 1703, 2076
Bse21I CCTNAGG 1 cut(s) 1206
Bse3DI GCAATG 2 cut(s) 501, 617
Bse8I GATNNNNATC 2 cut(s) 1605, 2105
BseBI CCWGG 4 cut(s) 900, 1683, 1770, 2198
BseCI ATCGAT 2 cut(s) 522, 2099
BseDI CCNNGG 4 cut(s) 482, 899, 1085, 1292
BseGI GGATG 1 cut(s) 2163
BseJI GATNNNNATC 2 cut(s) 1605, 2105
BseLI CCNNNNNNNGG 3 cut(s) 348, 1542, 1555
BseMI GCAATG 2 cut(s) 501, 617
BseMII CTCAG 5 cut(s) 118, 241, 578, 1220, 2018
BseNI ACTGG 5 cut(s) 755, 1039, 1644, 1703, 2076
BseSI GKGCMC 3 cut(s) 1575, 1644, 1746
BseXI GCAGC 5 cut(s) 112, 654, 817, 1234, 2222
BsgI GTGCAG 2 cut(s) 816, 1061
Bsh1285I CGRYCG 2 cut(s) 1275, 2103
BshFI GGCC 4 cut(s) 186, 1332, 1739, 1896
BshNI GGYRCC 1 cut(s) 1545
BshVI ATCGAT 2 cut(s) 522, 2099
BsiEI CGRYCG 2 cut(s) 1275, 2103
BsiHKAI GWGCWC 5 cut(s) 228, 455, 683, 1746, 1821
BsiHKCI CYCGRG 1 cut(s) 1958
BsiSI CCGG 6 cut(s) 744, 1345, 1519, 1657, 1758, 2063
BslFI GGGAC 2 cut(s) 1468, 1663
BslI CCNNNNNNNGG 3 cut(s) 348, 1542, 1555
BsmAI GTCTC 3 cut(s) 1583, 2097, 2211
BsmFI GGGAC 2 cut(s) 1468, 1663
BsnI GGCC 4 cut(s) 186, 1332, 1739, 1896
Bso31I GGTCTC 1 cut(s) 2211
BsoBI CYCGRG 1 cut(s) 1958
Bsp119I TTCGAA 1 cut(s) 1504
Bsp1286I GDGCHC 7 cut(s) 228, 455, 683, 1575, 1644, 1746, 1821
Bsp1407I TGTACA 1 cut(s) 2118
Bsp143I GATC 7 cut(s) 254, 1077, 1119, 1525, 1660, 2100, 2106
BspACI CCGC 2 cut(s) 492, 1786
BspANI GGCC 4 cut(s) 186, 1332, 1739, 1896
BspCNI CTCAG 5 cut(s) 117, 240, 579, 1219, 2019
BspDI ATCGAT 2 cut(s) 522, 2099
BspLI GGNNCC 2 cut(s) 399, 1547
BspMAI CTGCAG 2 cut(s) 102, 1193
BspPI GGATC 4 cut(s) 249, 1127, 1533, 1668
BspQI GCTCTTC 1 cut(s) 1356
BspT104I TTCGAA 1 cut(s) 1504
BspT107I GGYRCC 1 cut(s) 1545
BspTNI GGTCTC 1 cut(s) 2211
BsrDI GCAATG 2 cut(s) 501, 617
BsrFI RCCGGY 1 cut(s) 2062
BsrGI TGTACA 1 cut(s) 2118
BsrI ACTGG 5 cut(s) 755, 1039, 1644, 1703, 2076
BssAI RCCGGY 1 cut(s) 2062
BssECI CCNNGG 4 cut(s) 482, 899, 1085, 1292
BssMI GATC 7 cut(s) 254, 1077, 1119, 1525, 1660, 2100, 2106
BssT1I CCWWGG 3 cut(s) 482, 1085, 1292
Bst2UI CCWGG 4 cut(s) 900, 1683, 1770, 2198
Bst4CI ACNGT 6 cut(s) 149, 359, 1577, 1823, 1851, 2181
Bst6I CTCTTC 3 cut(s) 470, 842, 1356
BstAPI GCANNNNNTGC 1 cut(s) 1228
BstAUI TGTACA 1 cut(s) 2118
BstBI TTCGAA 1 cut(s) 1504
BstC8I GCNNGC 2 cut(s) 893, 1478
BstDEI CTNAG 9 cut(s) 104, 115, 227, 371, 587, 1154, 1206, 1734, 2027
BstF5I GGATG 1 cut(s) 2163
BstKTI GATC 7 cut(s) 257, 1080, 1122, 1528, 1663, 2103, 2109
BstMAI GTCTC 3 cut(s) 1583, 2097, 2211
BstMBI GATC 7 cut(s) 254, 1077, 1119, 1525, 1660, 2100, 2106
BstMCI CGRYCG 2 cut(s) 1275, 2103
BstMWI GCNNNNNNNGC 8 cut(s) 100, 109, 697, 1048, 1228, 1537, 1816, 2072
BstNI CCWGG 4 cut(s) 900, 1683, 1770, 2198
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 5 cut(s) 898, 1656, 1681, 1768, 2196
BstSFI CTRYAG 2 cut(s) 98, 1189
BstSLI GKGCMC 3 cut(s) 1575, 1644, 1746
BstV1I GCAGC 5 cut(s) 112, 654, 817, 1234, 2222
BstV2I GAAGAC 3 cut(s) 420, 1447, 1799
BstX2I RGATCY 2 cut(s) 1119, 1525
BstYI RGATCY 2 cut(s) 1119, 1525
Bsu15I ATCGAT 2 cut(s) 522, 2099
Bsu36I CCTNAGG 1 cut(s) 1206
BsuI GTATCC 1 cut(s) 1940
BsuRI GGCC 4 cut(s) 186, 1332, 1739, 1896
BsuTUI ATCGAT 2 cut(s) 522, 2099
BtgZI GCGATG 1 cut(s) 515
BtsCI GGATG 1 cut(s) 2163
BtsIMutI CAGTG 4 cut(s) 762, 1248, 1573, 1651
Cac8I GCNNGC 2 cut(s) 893, 1478
CaiI CAGNNNCTG 1 cut(s) 2213
Cfr10I RCCGGY 1 cut(s) 2062
Cfr13I GGNCC 2 cut(s) 398, 2172
ClaI ATCGAT 2 cut(s) 522, 2099
Csp6I GTAC 4 cut(s) 121, 1151, 1495, 2119
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviAII CATG 5 cut(s) 201, 715, 975, 1052, 1905
CviQI GTAC 4 cut(s) 121, 1151, 1495, 2119
DdeI CTNAG 9 cut(s) 104, 115, 227, 371, 587, 1154, 1206, 1734, 2027
DpnI GATC 7 cut(s) 256, 1079, 1121, 1527, 1662, 2102, 2108
DpnII GATC 7 cut(s) 254, 1077, 1119, 1525, 1660, 2100, 2106
EaeI YGGCCR 1 cut(s) 184
Eam1104I CTCTTC 3 cut(s) 470, 842, 1356
EarI CTCTTC 3 cut(s) 470, 842, 1356
Ecl136II GAGCTC 2 cut(s) 453, 681
Eco130I CCWWGG 3 cut(s) 482, 1085, 1292
Eco147I AGGCCT 1 cut(s) 1332
Eco24I GRGCYC 2 cut(s) 455, 683
Eco31I GGTCTC 1 cut(s) 2211
Eco32I GATATC 1 cut(s) 1215
Eco47I GGWCC 2 cut(s) 398, 2172
Eco53kI GAGCTC 2 cut(s) 453, 681
Eco57I CTGAAG 2 cut(s) 1479, 1811
Eco81I CCTNAGG 1 cut(s) 1206
Eco88I CYCGRG 1 cut(s) 1958
EcoICRI GAGCTC 2 cut(s) 453, 681
EcoRI GAATTC 1 cut(s) 1252
EcoRII CCWGG 4 cut(s) 898, 1681, 1768, 2196
EcoRV GATATC 1 cut(s) 1215
EcoT14I CCWWGG 3 cut(s) 482, 1085, 1292
EcoT22I ATGCAT 1 cut(s) 2129
EcoT38I GRGCYC 2 cut(s) 455, 683
ErhI CCWWGG 3 cut(s) 482, 1085, 1292
FaeI CATG 5 cut(s) 204, 718, 978, 1055, 1908
FalI AAGNNNNNCTT 4 cut(s) 286, 318, 1881, 1913
FaqI GGGAC 2 cut(s) 1468, 1663
FatI CATG 5 cut(s) 200, 714, 974, 1051, 1904
FauNDI CATATG 1 cut(s) 1420
FbaI TGATCA 1 cut(s) 1077
Fnu4HI GCNGC 5 cut(s) 101, 643, 831, 1223, 2211
FokI GGATG 1 cut(s) 2150
FriOI GRGCYC 2 cut(s) 455, 683
Fsp4HI GCNGC 5 cut(s) 101, 643, 831, 1223, 2211
FspBI CTAG 6 cut(s) 56, 134, 656, 1139, 1721, 2072
GluI GCNGC 5 cut(s) 101, 643, 831, 1223, 2211
HaeIII GGCC 4 cut(s) 186, 1332, 1739, 1896
HapII CCGG 6 cut(s) 744, 1345, 1519, 1657, 1758, 2063
Hin1II CATG 5 cut(s) 204, 718, 978, 1055, 1908
HincII GTYRAC 1 cut(s) 1032
HindII GTYRAC 1 cut(s) 1032
HindIII AAGCTT 1 cut(s) 1538
HpaII CCGG 6 cut(s) 744, 1345, 1519, 1657, 1758, 2063
HphI GGTGA 3 cut(s) 251, 974, 1421
Hpy166II GTNNAC 7 cut(s) 193, 401, 779, 1032, 1497, 1592, 1744
Hpy188III TCNNGA 6 cut(s) 106, 407, 443, 1580, 2104, 2135
Hpy8I GTNNAC 7 cut(s) 193, 401, 779, 1032, 1497, 1592, 1744
HpyAV CCTTC 4 cut(s) 605, 748, 981, 1406
HpyCH4III ACNGT 6 cut(s) 149, 359, 1577, 1823, 1851, 2181
HpyCH4IV ACGT 2 cut(s) 686, 1509
HpyF10VI GCNNNNNNNGC 8 cut(s) 100, 109, 697, 1048, 1228, 1537, 1816, 2072
HpyF3I CTNAG 9 cut(s) 104, 115, 227, 371, 587, 1154, 1206, 1734, 2027
HpySE526I ACGT 2 cut(s) 686, 1509
Hsp92II CATG 5 cut(s) 204, 718, 978, 1055, 1908
Ksp22I TGATCA 1 cut(s) 1077
Kzo9I GATC 7 cut(s) 254, 1077, 1119, 1525, 1660, 2100, 2106
LguI GCTCTTC 1 cut(s) 1356
LmnI GCTCC 3 cut(s) 42, 173, 1236
Lsp1109I GCAGC 5 cut(s) 112, 654, 817, 1234, 2222
LweI GCATC 5 cut(s) 399, 492, 1463, 1921, 2030
MaeI CTAG 6 cut(s) 56, 134, 656, 1139, 1721, 2072
MaeII ACGT 2 cut(s) 686, 1509
MaeIII GTNAC 5 cut(s) 149, 334, 798, 857, 1838
MalI GATC 7 cut(s) 256, 1079, 1121, 1527, 1662, 2102, 2108
MboI GATC 7 cut(s) 254, 1077, 1119, 1525, 1660, 2100, 2106
MflI RGATCY 2 cut(s) 1119, 1525
MhlI GDGCHC 7 cut(s) 228, 455, 683, 1575, 1644, 1746, 1821
MlsI TGGCCA 1 cut(s) 186
MluCI AATT 9 cut(s) 138, 219, 529, 1021, 1146, 1252, 1500, 1716, 1832
MluNI TGGCCA 1 cut(s) 186
MlyI GAGTC 2 cut(s) 2099, 2147
MmeI TCCRAC 4 cut(s) 282, 588, 1503, 1951
Mox20I TGGCCA 1 cut(s) 186
Mph1103I ATGCAT 1 cut(s) 2129
MroXI GAANNNNTTC 3 cut(s) 18, 1097, 1385
MscI TGGCCA 1 cut(s) 186
MseI TTAA 8 cut(s) 249, 516, 536, 638, 773, 980, 1145, 1512
MslI CAYNNNNRTG 1 cut(s) 794
Msp20I TGGCCA 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 2213
MspI CCGG 6 cut(s) 744, 1345, 1519, 1657, 1758, 2063
MspR9I CCNGG 5 cut(s) 900, 1658, 1683, 1770, 2198
MvaI CCWGG 4 cut(s) 900, 1683, 1770, 2198
MwoI GCNNNNNNNGC 8 cut(s) 100, 109, 697, 1048, 1228, 1537, 1816, 2072
NciI CCSGG 1 cut(s) 1658
NdeI CATATG 1 cut(s) 1420
NdeII GATC 7 cut(s) 254, 1077, 1119, 1525, 1660, 2100, 2106
NlaIII CATG 5 cut(s) 204, 718, 978, 1055, 1908
NlaIV GGNNCC 2 cut(s) 399, 1547
NmuCI GTSAC 4 cut(s) 149, 334, 798, 1838
NsiI ATGCAT 1 cut(s) 2129
NspI RCATGY 1 cut(s) 204
NspV TTCGAA 1 cut(s) 1504
PaeR7I CTCGAG 1 cut(s) 1958
PceI AGGCCT 1 cut(s) 1332
PciSI GCTCTTC 1 cut(s) 1356
PcsI WCGNNNNNNNCGW 2 cut(s) 1965, 2109
PdmI GAANNNNTTC 3 cut(s) 18, 1097, 1385
PflMI CCANNNNNTGG 1 cut(s) 1555
PfoI TCCNGGA 3 cut(s) 1656, 1681, 2196
PkrI GCNGC 5 cut(s) 102, 644, 832, 1224, 2212
Ple19I CGATCG 1 cut(s) 2103
PleI GAGTC 2 cut(s) 2098, 2146
PpsI GAGTC 2 cut(s) 2098, 2146
PshBI ATTAAT 1 cut(s) 1145
Psp124BI GAGCTC 2 cut(s) 455, 683
Psp1406I AACGTT 1 cut(s) 1509
Psp6I CCWGG 4 cut(s) 898, 1681, 1768, 2196
PspGI CCWGG 4 cut(s) 898, 1681, 1768, 2196
PspN4I GGNNCC 2 cut(s) 399, 1547
PspPI GGNCC 2 cut(s) 398, 2172
PspXI VCTCGAGB 1 cut(s) 1958
PstI CTGCAG 2 cut(s) 102, 1193
PstNI CAGNNNCTG 1 cut(s) 2213
PsuI RGATCY 2 cut(s) 1119, 1525
PvuI CGATCG 1 cut(s) 2103
PvuII CAGCTG 1 cut(s) 2213
RsaI GTAC 4 cut(s) 122, 1152, 1496, 2120
RsaNI GTAC 4 cut(s) 121, 1151, 1495, 2119
RseI CAYNNNNRTG 1 cut(s) 794
SacI GAGCTC 2 cut(s) 455, 683
SapI GCTCTTC 1 cut(s) 1356
SaqAI TTAA 8 cut(s) 249, 516, 536, 638, 773, 980, 1145, 1512
SatI GCNGC 5 cut(s) 101, 643, 831, 1223, 2211
Sau3AI GATC 7 cut(s) 254, 1077, 1119, 1525, 1660, 2100, 2106
Sau96I GGNCC 2 cut(s) 398, 2172
SchI GAGTC 2 cut(s) 2099, 2147
ScrFI CCNGG 5 cut(s) 900, 1658, 1683, 1770, 2198
SduI GDGCHC 7 cut(s) 228, 455, 683, 1575, 1644, 1746, 1821
SfaNI GCATC 5 cut(s) 399, 492, 1463, 1921, 2030
SfcI CTRYAG 2 cut(s) 98, 1189
Sfr274I CTCGAG 1 cut(s) 1958
SfuI TTCGAA 1 cut(s) 1504
SinI GGWCC 2 cut(s) 398, 2172
SlaI CTCGAG 1 cut(s) 1958
SmiMI CAYNNNNRTG 1 cut(s) 794
SmlI CTYRAG 3 cut(s) 434, 592, 1958
SmoI CTYRAG 3 cut(s) 434, 592, 1958
Sse9I AATT 9 cut(s) 138, 219, 529, 1021, 1146, 1252, 1500, 1716, 1832
SseBI AGGCCT 1 cut(s) 1332
SsiI CCGC 2 cut(s) 492, 1786
SspI AATATT 1 cut(s) 853
SspMI CTAG 6 cut(s) 56, 134, 656, 1139, 1721, 2072
SstI GAGCTC 2 cut(s) 455, 683
StuI AGGCCT 1 cut(s) 1332
StyD4I CCNGG 5 cut(s) 898, 1656, 1681, 1768, 2196
StyI CCWWGG 3 cut(s) 482, 1085, 1292
TaaI ACNGT 6 cut(s) 149, 359, 1577, 1823, 1851, 2181
TaiI ACGT 2 cut(s) 689, 1512
TaqII GACCGA 1 cut(s) 1289
TasI AATT 9 cut(s) 138, 219, 529, 1021, 1146, 1252, 1500, 1716, 1832
TatI WGTACW 3 cut(s) 1150, 1494, 2118
Tru1I TTAA 8 cut(s) 249, 516, 536, 638, 773, 980, 1145, 1512
Tru9I TTAA 8 cut(s) 249, 516, 536, 638, 773, 980, 1145, 1512
TscAI CASTG 4 cut(s) 762, 1248, 1580, 1651
TseFI GTSAC 4 cut(s) 149, 334, 798, 1838
TseI GCWGC 5 cut(s) 100, 642, 830, 1222, 2210
Tsp45I GTSAC 4 cut(s) 149, 334, 798, 1838
TspDTI ATGAA 6 cut(s) 407, 922, 963, 1002, 1068, 1407
TspGWI ACGGA 1 cut(s) 2101
TspRI CASTG 4 cut(s) 762, 1248, 1580, 1651
Van91I CCANNNNNTGG 1 cut(s) 1555
VneI GTGCAC 1 cut(s) 1742
VpaK11BI GGWCC 2 cut(s) 398, 2172
VspI ATTAAT 1 cut(s) 1145
XapI RAATTY 3 cut(s) 138, 529, 1252
XceI RCATGY 1 cut(s) 204
XhoI CTCGAG 1 cut(s) 1958
XmnI GAANNNNTTC 3 cut(s) 18, 1097, 1385
XspI CTAG 6 cut(s) 56, 134, 656, 1139, 1721, 2072
Zsp2I ATGCAT 1 cut(s) 2129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.