Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0014194

Physical Location & Seq

Genomic Coordinates Forward (+)

18583272 .. 18586700

Gene Structure 3429 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1719 bp

ATGGGAGTTGGCACATATGGCTATCCTGAAGAGGCCACCATGAGGAGCGGTGGTGGCGGCGGAGGATATGACGTTCCCGATGGTGTCGACATTCGGGGACGGTACGACGGAGAGTTTGCGAGGATTCTGACCAAGGATGCTTTGCAGTTTGTGGTGGAGTTGCAGAGGGAATTTAGGAACCGAATCAAGTATGCGTTGGAGTGTCGGAAGGAGGCTAAGAGGAGGTACAATGAGGGGGCTTTGCCAGGGTTTGATCCTGCTACCAAGTTCATTAGGGAGGGGAATTGGGTTTGTGCTCCGGTACCGGAAGCGGTGGCGGATCGGAAGGTGGAGATTACGGGACCTGTCGAGAGGAAGATGATCATCAATGCTCTCAATTCTGGAGCCAAAGTTTTCATGGCGGACTTTGAAGATGCACTGTCACCAACTTGGGAGAACTTGATGAGAGGGCAAGTGAATCTGAAGGATGCTGTGGATGGGAGCATAACATTCCATGACAAGGCCAGGAATAGGGTTTACAAGCTGAACGACAGAAACACAGCCAAGCTTTTTGTTCGTCCGAGAGGTTGGCACTTGCCTGAGGCTCACATTTTCATTGATGGTGAGCCTGCAACGGGTTGCCTTGTGGACTTCGGCCTTTACTTTTACCACAACTATAAAGCATTCAGGCAAACCCAAGGGGCTGGATTTGGACCTTTCTTCTATCTCCCTAAAATGGAGACCTCAAGGGAAGCTAAGATATGGAACTGTGTGTTTGAGAGGGCAGAGAAGATGGCAGGGATAGAGAGAGGAAGCATCAGGGCAACTGTGCTGATTGAAACACTTCCAGCTGTGTTTCAAATGGATGAGATCCTCTATGAGCTGAGGGACCACTCTGTTGGCTTGAACTGTGGAAGATGGGATTACATTTTCAGCTATGTCAAAACCTTCCAGGCTCACCCCGACCGCCTCTTGCCAGACCGTGTTCTGGTGGGCATGACTCAACATTTCATGAGGAGCTACTCTGACCTCCTCATCCGGACGTGCCATAGACGCGGCGTGCACGCCATGGGTGGCATGGCAGCTCAGATTCCAATCAGAGATGATCCAAAAGCAAATGAAGCAGCCTTGGACTTGGTGAGGAAAGACAAGCTAAGGGAGGTCAAAGCAGGACATGATGGAACATGGGCAGCTCACCCAGGGCTAATCCCAGCCTGCATGGATATCTTCACAAACAACATGCCACCCAACACCCCTAACCAAATCCAATCCATGAGAAGAGAAGATGCATCCACCATAACAGAAGAAGACCTCCTCCAGAGGCCTCGCGGCGTCCGAACCCTAGAAGGTCTCCGGCTCAACACCCGGGTAGGGATCCAGTACGTGGCAGCATGGCTCACTGGGACAGGCTCAGTGCCACTCTACAACCTCATGGAGGATGCAGCCACTGCGGAAATAAGCAGGGTGCAGAACTGGCAGTGGATCAAGTATGGAGTGGAGTTGGACGGTGACGGGCTCGGAGTGAGGGTTGGGAAGGAGCTGTTTGGGAGAGTGGTGGAGGAAGAAATGGAGAGGATTGAGAGGGAGGTTGGGAAGGAGAAGTTTAAGAAGGGAATGTATAAGGAGGCTTGCAGGATTTTCACCAGGCAATGCACTGCTCAAACACTAGATGATTTTCTCACTCTGGATGCCTACAATCGCATTGTGCTACACTACCCAAAGGGATCATCCAGGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004474 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005975 GO:0006081 GO:0006082 GO:0006097 GO:0008150 GO:0008152 GO:0009514 GO:0009987 GO:0016740 GO:0016746 GO:0019752 GO:0032787 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044262 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046487 GO:0046912 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00620 ko00630 ko01100 ko01110 ko01120 ko01200 map00620 map00630 map01100 map01110 map01120 map01200

Pfam Domains

Protein Families

Malate_synthase

Protein Analysis

573

Amino Acids

64.98

Weight (kDa)

8.23

Isoelectric Point (pI)

39.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_N	PF20656	27 - 89	1.5e-21	Malate synthase, N-terminal domain
MS_TIM-barrel	PF01274	182 - 433	8.3e-117	Malate synthase, TIM barrel domain
MS_C	PF20659	439 - 561	1.4e-44	Malate synthase, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014194)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03860 AT5G03860
fragaria_vesca	FvH4_5g07710
malus_domestica	MD06G1147700.v1.1
prunus_persica	Prupe.5G154600_v2.0.a1
pyrus_communis	pycom06g13710
rosa_chinensis	RchiOBHm_Chr7g0190721
rosa_laevigata	RLG00000004501
rosa_multiflora	Rmu_sc0030109.1_g000001 Rmu_sc0030110.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264350
rosa_rugosa	Rorug06G0506700
rosa_samantha	Rh7AG113300 Rh7BG115800 Rh7CG118300 Rh7DG117200
rosa_wichuraiana	Rw7G009890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	301
AccB1I	GGYRCC	1 cut(s)	301
AccB7I	CCANNNNNTGG	1 cut(s)	1363
AccBSI	CCGCTC	1 cut(s)	48
AccI	GTMKAC	1 cut(s)	87
AccII	CGCG	2 cut(s)	1035, 1308
AccIII	TCCGGA	1 cut(s)	1017
AclWI	GGATC	8 cut(s)	248, 327, 844, 1079, 1348, 1361, 1469, 1711
AcsI	RAATTY	1 cut(s)	170
AcuI	CTGAAG	2 cut(s)	48, 482
AcyI	GRCGYC	1 cut(s)	1311
AfaI	GTAC	4 cut(s)	104, 227, 303, 1361
AfiI	CCNNNNNNNGG	9 cut(s)	42, 499, 510, 614, 715, 967, 1350, 1363, 1414
AgsI	TTSAA	4 cut(s)	410, 818, 839, 886
AjiI	CACGTC	1 cut(s)	1023
AjnI	CCWGG	6 cut(s)	244, 503, 930, 1177, 1622, 1709
AloI	GAACNNNNNNTCC	2 cut(s)	57, 89
Alw21I	GWGCWC	2 cut(s)	298, 1044
Alw26I	GTCTC	2 cut(s)	713, 1334
Alw44I	GTGCAC	1 cut(s)	1040
AlwI	GGATC	8 cut(s)	248, 327, 844, 1079, 1348, 1361, 1469, 1711
AlwNI	CAGNNNCTG	2 cut(s)	1427, 1716
Ama87I	CYCGRG	1 cut(s)	1344
Aor13HI	TCCGGA	1 cut(s)	1017
AoxI	GGCC	4 cut(s)	33, 501, 634, 1301
ApaLI	GTGCAC	1 cut(s)	1040
ApeKI	GCWGC	5 cut(s)	1061, 1103, 1169, 1367, 1421
ApoI	RAATTY	1 cut(s)	170
ArsI	GACNNNNNNTTYG	2 cut(s)	98, 130
Asp700I	GAANNNNTTC	1 cut(s)	822
Asp718I	GGTACC	1 cut(s)	301
AspS9I	GGNCC	3 cut(s)	341, 692, 868
AsuC2I	CCSGG	2 cut(s)	1345, 1346
AsuHPI	GGTGA	7 cut(s)	414, 614, 929, 1129, 1166, 1499, 1612
AvaI	CYCGRG	1 cut(s)	1344
AvaII	GGWCC	3 cut(s)	341, 692, 868
AxyI	CCTNAGG	1 cut(s)	579
BaeGI	GKGCMC	1 cut(s)	1044
BamHI	GGATCC	1 cut(s)	1353
BanI	GGYRCC	1 cut(s)	301
BanII	GRGCYC	1 cut(s)	1497
BbsI	GAAGAC	1 cut(s)	1293
Bbv12I	GWGCWC	2 cut(s)	298, 1044
BbvCI	CCTCAGC	1 cut(s)	863
BbvI	GCAGC	5 cut(s)	1073, 1115, 1181, 1379, 1433
BccI	CCATC	6 cut(s)	74, 470, 593, 766, 891, 1151
BciT130I	CCWGG	6 cut(s)	246, 505, 932, 1179, 1624, 1711
BclI	TGATCA	1 cut(s)	360
BcnI	CCSGG	2 cut(s)	1345, 1346
BcoDI	GTCTC	2 cut(s)	713, 1334
BfaI	CTAG	2 cut(s)	1322, 1646
BisI	GCNGC	8 cut(s)	58, 1036, 1062, 1104, 1170, 1309, 1368, 1422
BlsI	GCNGC	8 cut(s)	59, 1037, 1063, 1105, 1171, 1310, 1369, 1423
Bme1390I	CCNGG	8 cut(s)	246, 505, 932, 1179, 1345, 1346, 1624, 1711
Bme18I	GGWCC	3 cut(s)	341, 692, 868
BmeT110I	CYCGRG	1 cut(s)	1344
BmgBI	CACGTC	1 cut(s)	1023
BmgT120I	GGNCC	3 cut(s)	341, 692, 868
BmiI	GGNNCC	6 cut(s)	179, 303, 342, 385, 869, 1355
BmrFI	CCNGG	8 cut(s)	246, 505, 932, 1179, 1345, 1346, 1624, 1711
BmrI	ACTGGG	1 cut(s)	1389
BmsI	GCATC	8 cut(s)	127, 403, 457, 804, 1255, 1277, 1408, 1657
BmuI	ACTGGG	1 cut(s)	1389
BpiI	GAAGAC	1 cut(s)	1293
BpmI	CTGGAG	2 cut(s)	402, 1280
Bpu10I	CCTNAGC	2 cut(s)	863, 1133
BpuEI	CTTGAG	1 cut(s)	709
BpuMI	CCSGG	2 cut(s)	1345, 1346
BsaAI	YACGTR	1 cut(s)	1363
BsaBI	GATNNNNATC	2 cut(s)	362, 1073
BsaHI	GRCGYC	1 cut(s)	1311
BsaI	GGTCTC	2 cut(s)	713, 1334
BsaJI	CCNNGG	8 cut(s)	132, 245, 676, 1047, 1107, 1177, 1178, 1344
BsaWI	WCCGGW	3 cut(s)	298, 304, 1017
BsaXI	ACNNNNNCTCC	6 cut(s)	37, 67, 375, 405, 1491, 1521
Bsc4I	CCNNNNNNNGG	9 cut(s)	42, 499, 510, 614, 715, 967, 1350, 1363, 1414
Bse1I	ACTGG	3 cut(s)	1357, 1384, 1457
Bse21I	CCTNAGG	1 cut(s)	579
Bse3DI	GCAATG	1 cut(s)	1634
Bse8I	GATNNNNATC	2 cut(s)	362, 1073
BseAI	TCCGGA	1 cut(s)	1017
BseBI	CCWGG	6 cut(s)	246, 505, 932, 1179, 1624, 1711
BseDI	CCNNGG	8 cut(s)	132, 245, 676, 1047, 1107, 1177, 1178, 1344
BseGI	GGATG	9 cut(s)	142, 472, 481, 850, 1014, 1268, 1423, 1672, 1706
BseJI	GATNNNNATC	2 cut(s)	362, 1073
BseLI	CCNNNNNNNGG	9 cut(s)	42, 499, 510, 614, 715, 967, 1350, 1363, 1414
BseMI	GCAATG	1 cut(s)	1634
BseMII	CTCAG	4 cut(s)	570, 854, 1079, 1404
BseNI	ACTGG	3 cut(s)	1357, 1384, 1457
BseRI	GAGGAG	5 cut(s)	58, 235, 1001, 1009, 1283
BseSI	GKGCMC	1 cut(s)	1044
BseXI	GCAGC	5 cut(s)	1073, 1115, 1181, 1379, 1433
BseYI	CCCAGC	1 cut(s)	1189
BsgI	GTGCAG	1 cut(s)	1466
Bsh1236I	CGCG	2 cut(s)	1035, 1308
Bsh1285I	CGRYCG	1 cut(s)	946
BshFI	GGCC	4 cut(s)	35, 503, 636, 1303
BshNI	GGYRCC	1 cut(s)	301
BsiEI	CGRYCG	1 cut(s)	946
BsiHKAI	GWGCWC	2 cut(s)	298, 1044
BsiHKCI	CYCGRG	1 cut(s)	1344
BsiSI	CCGG	5 cut(s)	299, 305, 1018, 1333, 1345
BslFI	GGGAC	4 cut(s)	111, 354, 881, 1396
BslI	CCNNNNNNNGG	9 cut(s)	42, 499, 510, 614, 715, 967, 1350, 1363, 1414
BsmAI	GTCTC	2 cut(s)	713, 1334
BsmFI	GGGAC	4 cut(s)	111, 354, 881, 1396
BsmI	GAATGC	1 cut(s)	662
BsnI	GGCC	4 cut(s)	35, 503, 636, 1303
Bso31I	GGTCTC	2 cut(s)	713, 1334
BsoBI	CYCGRG	1 cut(s)	1344
Bsp1286I	GDGCHC	3 cut(s)	298, 1044, 1497
Bsp13I	TCCGGA	1 cut(s)	1017
Bsp143I	GATC	8 cut(s)	253, 319, 360, 849, 1084, 1353, 1461, 1703
Bsp19I	CCATGG	1 cut(s)	1047
BspANI	GGCC	4 cut(s)	35, 503, 636, 1303
BspCNI	CTCAG	4 cut(s)	571, 855, 1078, 1403
BspEI	TCCGGA	1 cut(s)	1017
BspFNI	CGCG	2 cut(s)	1035, 1308
BspHI	TCATGA	1 cut(s)	990
BspLI	GGNNCC	6 cut(s)	179, 303, 342, 385, 869, 1355
BspPI	GGATC	8 cut(s)	248, 327, 844, 1079, 1348, 1361, 1469, 1711
BspT107I	GGYRCC	1 cut(s)	301
BspTNI	GGTCTC	2 cut(s)	713, 1334
BsrBI	CCGCTC	1 cut(s)	48
BsrDI	GCAATG	1 cut(s)	1634
BsrI	ACTGG	3 cut(s)	1357, 1384, 1457
BssECI	CCNNGG	8 cut(s)	132, 245, 676, 1047, 1107, 1177, 1178, 1344
BssMI	GATC	8 cut(s)	253, 319, 360, 849, 1084, 1353, 1461, 1703
BssNI	GRCGYC	1 cut(s)	1311
BssT1I	CCWWGG	4 cut(s)	132, 676, 1047, 1107
Bst2UI	CCWGG	6 cut(s)	246, 505, 932, 1179, 1624, 1711
Bst4CI	ACNGT	7 cut(s)	102, 420, 749, 808, 890, 962, 1487
Bst6I	CTCTTC	2 cut(s)	24, 1252
BstACI	GRCGYC	1 cut(s)	1311
BstAPI	GCANNNNNTGC	1 cut(s)	1427
BstBAI	YACGTR	1 cut(s)	1363
BstC8I	GCNNGC	5 cut(s)	609, 1040, 1044, 1195, 1609
BstDEI	CTNAG	7 cut(s)	216, 579, 735, 863, 1065, 1133, 1390
BstDSI	CCRYGG	1 cut(s)	1047
BstENI	CCTNNNNNAGG	1 cut(s)	1412
BstF5I	GGATG	9 cut(s)	142, 472, 481, 850, 1014, 1268, 1423, 1672, 1706
BstFNI	CGCG	2 cut(s)	1035, 1308
BstKTI	GATC	8 cut(s)	256, 322, 363, 852, 1087, 1356, 1464, 1706
BstMAI	GTCTC	2 cut(s)	713, 1334
BstMBI	GATC	8 cut(s)	253, 319, 360, 849, 1084, 1353, 1461, 1703
BstMCI	CGRYCG	1 cut(s)	946
BstMWI	GCNNNNNNNGC	6 cut(s)	18, 54, 1032, 1100, 1427, 1453
BstNI	CCWGG	6 cut(s)	246, 505, 932, 1179, 1624, 1711
BstNSI	RCATGY	1 cut(s)	1222
BstSCI	CCNGG	8 cut(s)	244, 503, 930, 1177, 1343, 1344, 1622, 1709
BstSLI	GKGCMC	1 cut(s)	1044
BstUI	CGCG	2 cut(s)	1035, 1308
BstV1I	GCAGC	5 cut(s)	1073, 1115, 1181, 1379, 1433
BstV2I	GAAGAC	1 cut(s)	1293
BstX2I	RGATCY	2 cut(s)	849, 1353
BstXI	CCANNNNNNTGG	2 cut(s)	683, 878
BstYI	RGATCY	2 cut(s)	849, 1353
Bsu36I	CCTNAGG	1 cut(s)	579
BsuRI	GGCC	4 cut(s)	35, 503, 636, 1303
BtgI	CCRYGG	1 cut(s)	1047
BtrI	CACGTC	1 cut(s)	1023
BtsCI	GGATG	9 cut(s)	142, 472, 481, 850, 1014, 1268, 1423, 1672, 1706
BtsI	GCAGTG	3 cut(s)	1425, 1463, 1632
BtsIMutI	CAGTG	6 cut(s)	416, 1377, 1398, 1425, 1463, 1632
Cac8I	GCNNGC	5 cut(s)	609, 1040, 1044, 1195, 1609
CaiI	CAGNNNCTG	2 cut(s)	1427, 1716
CciI	TCATGA	1 cut(s)	990
Cfr13I	GGNCC	3 cut(s)	341, 692, 868
Cfr9I	CCCGGG	1 cut(s)	1344
CseI	GACGC	2 cut(s)	1041, 1300
Csp6I	GTAC	4 cut(s)	103, 226, 302, 1360
CviQI	GTAC	4 cut(s)	103, 226, 302, 1360
DdeI	CTNAG	7 cut(s)	216, 579, 735, 863, 1065, 1133, 1390
DpnI	GATC	8 cut(s)	255, 321, 362, 851, 1086, 1355, 1463, 1705
DpnII	GATC	8 cut(s)	253, 319, 360, 849, 1084, 1353, 1461, 1703
Eam1104I	CTCTTC	2 cut(s)	24, 1252
EarI	CTCTTC	2 cut(s)	24, 1252
EciI	GGCGGA	3 cut(s)	75, 332, 416
Eco130I	CCWWGG	4 cut(s)	132, 676, 1047, 1107
Eco147I	AGGCCT	1 cut(s)	1303
Eco24I	GRGCYC	1 cut(s)	1497
Eco31I	GGTCTC	2 cut(s)	713, 1334
Eco32I	GATATC	1 cut(s)	1204
Eco47I	GGWCC	3 cut(s)	341, 692, 868
Eco57I	CTGAAG	2 cut(s)	48, 482
Eco81I	CCTNAGG	1 cut(s)	579
Eco88I	CYCGRG	1 cut(s)	1344
EcoNI	CCTNNNNNAGG	1 cut(s)	1412
EcoO109I	RGGNCCY	1 cut(s)	341
EcoRII	CCWGG	6 cut(s)	244, 503, 930, 1177, 1622, 1709
EcoRV	GATATC	1 cut(s)	1204
EcoT14I	CCWWGG	4 cut(s)	132, 676, 1047, 1107
EcoT22I	ATGCAT	1 cut(s)	1270
EcoT38I	GRGCYC	1 cut(s)	1497
ErhI	CCWWGG	4 cut(s)	132, 676, 1047, 1107
FaqI	GGGAC	4 cut(s)	111, 354, 881, 1396
FauNDI	CATATG	1 cut(s)	16
FbaI	TGATCA	1 cut(s)	360
FblI	GTMKAC	1 cut(s)	87
Fnu4HI	GCNGC	8 cut(s)	58, 1036, 1062, 1104, 1170, 1309, 1368, 1422
FokI	GGATG	9 cut(s)	149, 479, 488, 857, 1001, 1255, 1430, 1679, 1693
FriOI	GRGCYC	1 cut(s)	1497
Fsp4HI	GCNGC	8 cut(s)	58, 1036, 1062, 1104, 1170, 1309, 1368, 1422
FspBI	CTAG	2 cut(s)	1322, 1646
GluI	GCNGC	8 cut(s)	58, 1036, 1062, 1104, 1170, 1309, 1368, 1422
GsaI	CCCAGC	1 cut(s)	1193
GsuI	CTGGAG	2 cut(s)	402, 1280
HaeIII	GGCC	4 cut(s)	35, 503, 636, 1303
HapII	CCGG	5 cut(s)	299, 305, 1018, 1333, 1345
HgaI	GACGC	2 cut(s)	1041, 1300
Hin1I	GRCGYC	1 cut(s)	1311
HincII	GTYRAC	1 cut(s)	88
HindII	GTYRAC	1 cut(s)	88
HindIII	AAGCTT	1 cut(s)	545
HinfI	GANTC	5 cut(s)	124, 183, 457, 979, 1069
HpaII	CCGG	5 cut(s)	299, 305, 1018, 1333, 1345
HphI	GGTGA	7 cut(s)	414, 614, 929, 1129, 1166, 1499, 1612
Hpy166II	GTNNAC	4 cut(s)	88, 517, 628, 1042
Hpy188III	TCNNGA	8 cut(s)	26, 77, 349, 381, 991, 1018, 1297, 1664
Hpy8I	GTNNAC	4 cut(s)	88, 517, 628, 1042
Hpy99I	CGWCG	1 cut(s)	110
HpyAV	CCTTC	8 cut(s)	202, 319, 457, 937, 1319, 1507, 1567, 1582
HpyCH4III	ACNGT	7 cut(s)	102, 420, 749, 808, 890, 962, 1487
HpyCH4IV	ACGT	3 cut(s)	72, 1022, 1362
HpyF10VI	GCNNNNNNNGC	6 cut(s)	18, 54, 1032, 1100, 1427, 1453
HpyF3I	CTNAG	7 cut(s)	216, 579, 735, 863, 1065, 1133, 1390
HpySE526I	ACGT	3 cut(s)	72, 1022, 1362
Hsp92I	GRCGYC	1 cut(s)	1311
Kpn2I	TCCGGA	1 cut(s)	1017
KpnI	GGTACC	1 cut(s)	305
Ksp22I	TGATCA	1 cut(s)	360
Kzo9I	GATC	8 cut(s)	253, 319, 360, 849, 1084, 1353, 1461, 1703
LmnI	GCTCC	6 cut(s)	45, 301, 383, 480, 996, 1516
Lsp1109I	GCAGC	5 cut(s)	1073, 1115, 1181, 1379, 1433
LweI	GCATC	8 cut(s)	127, 403, 457, 804, 1255, 1277, 1408, 1657
MaeI	CTAG	2 cut(s)	1322, 1646
MaeII	ACGT	3 cut(s)	72, 1022, 1362
MaeIII	GTNAC	2 cut(s)	420, 1487
MalI	GATC	8 cut(s)	255, 321, 362, 851, 1086, 1355, 1463, 1705
MbiI	CCGCTC	1 cut(s)	48
MboI	GATC	8 cut(s)	253, 319, 360, 849, 1084, 1353, 1461, 1703
MflI	RGATCY	2 cut(s)	849, 1353
MhlI	GDGCHC	3 cut(s)	298, 1044, 1497
MluCI	AATT	3 cut(s)	170, 283, 376
MlyI	GAGTC	1 cut(s)	973
MmeI	TCCRAC	3 cut(s)	177, 185, 1461
Mph1103I	ATGCAT	1 cut(s)	1270
MroI	TCCGGA	1 cut(s)	1017
MroXI	GAANNNNTTC	1 cut(s)	822
MseI	TTAA	1 cut(s)	1584
MspA1I	CMGCKG	1 cut(s)	830
MspI	CCGG	5 cut(s)	299, 305, 1018, 1333, 1345
MspR9I	CCNGG	8 cut(s)	246, 505, 932, 1179, 1345, 1346, 1624, 1711
Mva1269I	GAATGC	1 cut(s)	662
MvaI	CCWGG	6 cut(s)	246, 505, 932, 1179, 1624, 1711
MvnI	CGCG	2 cut(s)	1035, 1308
MwoI	GCNNNNNNNGC	6 cut(s)	18, 54, 1032, 1100, 1427, 1453
NciI	CCSGG	2 cut(s)	1345, 1346
NcoI	CCATGG	1 cut(s)	1047
NdeI	CATATG	1 cut(s)	16
NdeII	GATC	8 cut(s)	253, 319, 360, 849, 1084, 1353, 1461, 1703
NlaIV	GGNNCC	6 cut(s)	179, 303, 342, 385, 869, 1355
NmuCI	GTSAC	2 cut(s)	420, 1487
NsiI	ATGCAT	1 cut(s)	1270
NspI	RCATGY	1 cut(s)	1222
PagI	TCATGA	1 cut(s)	990
PasI	CCCWGGG	1 cut(s)	1178
PceI	AGGCCT	1 cut(s)	1303
PcsI	WCGNNNNNNNCGW	1 cut(s)	1312
PctI	GAATGC	1 cut(s)	662
PdmI	GAANNNNTTC	1 cut(s)	822
PfeI	GAWTC	4 cut(s)	124, 183, 457, 1069
PflMI	CCANNNNNTGG	1 cut(s)	1363
PkrI	GCNGC	8 cut(s)	59, 1037, 1063, 1105, 1171, 1310, 1369, 1423
PleI	GAGTC	1 cut(s)	973
PpsI	GAGTC	1 cut(s)	973
Ppu21I	YACGTR	1 cut(s)	1363
PpuMI	RGGWCCY	1 cut(s)	341
Psp5II	RGGWCCY	1 cut(s)	341
Psp6I	CCWGG	6 cut(s)	244, 503, 930, 1177, 1622, 1709
PspFI	CCCAGC	1 cut(s)	1189
PspGI	CCWGG	6 cut(s)	244, 503, 930, 1177, 1622, 1709
PspN4I	GGNNCC	6 cut(s)	179, 303, 342, 385, 869, 1355
PspPI	GGNCC	3 cut(s)	341, 692, 868
PspPPI	RGGWCCY	1 cut(s)	341
PstNI	CAGNNNCTG	2 cut(s)	1427, 1716
PsuI	RGATCY	2 cut(s)	849, 1353
PvuII	CAGCTG	1 cut(s)	830
RsaI	GTAC	4 cut(s)	104, 227, 303, 1361
RsaNI	GTAC	4 cut(s)	103, 226, 302, 1360
SalI	GTCGAC	1 cut(s)	86
SaqAI	TTAA	1 cut(s)	1584
SatI	GCNGC	8 cut(s)	58, 1036, 1062, 1104, 1170, 1309, 1368, 1422
Sau3AI	GATC	8 cut(s)	253, 319, 360, 849, 1084, 1353, 1461, 1703
Sau96I	GGNCC	3 cut(s)	341, 692, 868
SchI	GAGTC	1 cut(s)	973
ScrFI	CCNGG	8 cut(s)	246, 505, 932, 1179, 1345, 1346, 1624, 1711
SduI	GDGCHC	3 cut(s)	298, 1044, 1497
SfaNI	GCATC	8 cut(s)	127, 403, 457, 804, 1255, 1277, 1408, 1657
SinI	GGWCC	3 cut(s)	341, 692, 868
SmaI	CCCGGG	1 cut(s)	1346
SmlI	CTYRAG	1 cut(s)	724
SmoI	CTYRAG	1 cut(s)	724
Sse9I	AATT	3 cut(s)	170, 283, 376
SseBI	AGGCCT	1 cut(s)	1303
SspMI	CTAG	2 cut(s)	1322, 1646
StuI	AGGCCT	1 cut(s)	1303
StyD4I	CCNGG	8 cut(s)	244, 503, 930, 1177, 1343, 1344, 1622, 1709
StyI	CCWWGG	4 cut(s)	132, 676, 1047, 1107
TaaI	ACNGT	7 cut(s)	102, 420, 749, 808, 890, 962, 1487
TaiI	ACGT	3 cut(s)	75, 1025, 1365
TaqI	TCGA	2 cut(s)	87, 348
TasI	AATT	3 cut(s)	170, 283, 376
TauI	GCSGC	3 cut(s)	60, 1038, 1311
TfiI	GAWTC	4 cut(s)	124, 183, 457, 1069
Tru1I	TTAA	1 cut(s)	1584
Tru9I	TTAA	1 cut(s)	1584
TscAI	CASTG	6 cut(s)	423, 1384, 1398, 1432, 1463, 1639
TseFI	GTSAC	2 cut(s)	420, 1487
TseI	GCWGC	5 cut(s)	1061, 1103, 1169, 1367, 1421
Tsp45I	GTSAC	2 cut(s)	420, 1487
TspDTI	ATGAA	5 cut(s)	259, 385, 583, 979, 1113
TspGWI	ACGGA	1 cut(s)	123
TspMI	CCCGGG	1 cut(s)	1344
TspRI	CASTG	6 cut(s)	423, 1384, 1398, 1432, 1463, 1639
Van91I	CCANNNNNTGG	1 cut(s)	1363
VneI	GTGCAC	1 cut(s)	1040
VpaK11BI	GGWCC	3 cut(s)	341, 692, 868
XagI	CCTNNNNNAGG	1 cut(s)	1412
XapI	RAATTY	1 cut(s)	170
XceI	RCATGY	1 cut(s)	1222
XcmI	CCANNNNNNNNNTGG	2 cut(s)	394, 1054
XmaI	CCCGGG	1 cut(s)	1344
XmiI	GTMKAC	1 cut(s)	87
XmnI	GAANNNNTTC	1 cut(s)	822
XspI	CTAG	2 cut(s)	1322, 1646
Zsp2I	ATGCAT	1 cut(s)	1270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.