pycom06g17190

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014174
Physical Location & Seq
Forward (+)
21220408 .. 21222146
1739 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1200 bp
ATGTCTCCCACATTGTGTTTAATCCAAAACCCAAGTCTTCCATCTCACATTGGAAGTCCTGAAAATATCTGCATTTTCTCGAGTTTTTCAACCATGAAGTTCACAGCTCTAGCAGCAATGATGGCGGCCGTGACTCTCTTCGGCCTCCTCATTGATGGCCACGACTGCAAAATCGTGCAGTTTATATTCGGGGACTCCCTCTCCGACGTTGGAAACAACAAGCAAATGAGCAGGAGTCTCGCCCAAGCAAGCCTGCCTTGGTATGGCATTGATATGGGCAATGGACTGCCCAATGGCAGGTTCTCTAATGGCCGTACAGTTGCTGATATAATAGGTGATAACATGGGGCTCCCAAGGCCGCCGGCCTTTCTTGATCCGTCATTGACCGATGACATGATACTTGAAAAAGGAGTAAACTATGCCTCTGGTGGTGGAGGGATTTTGAATGAAACCGGCGGCTACTTTATTCAGAGGTTCTCGCTCTACAAGCAGATCGATCTGTTTCAAGGGACACAAACACTGATCAAAAACAAAATTGGCGCAGACGCAGCGGAGAAATTCTTCCAAGACGCTCGTTATGTGGTCGCTTTAGGAAGCAACGACTTCATTAACAACTACTTGATGCCTGTTTACAGAGATTCGTGGAGTTACAACGATCAAACTTTCATCGAATACCTAATGGGAACTCTAGAAAGACAACTTAATCTGTTGCACAGCCTAGGGGCGAGGCAGCTGATAGTGTTTGGGCTAGGACCAATGGGTTGTATTCCGCTCCAAAGGGTGCTAAGTACCACTTACGGTTGCCAAGAAAGGTCAAACAAACTAGCTCTCAGCTTCAACAAAGCTGGAAGCAAGCTAATGGAAGACTTGTCCAGCAAGCTTCCTAACGCAAGCTATCGATTTGGTGATGCTTATGATGTCGTCAACGATGTGATCAGTAATCCAAACGATTATGGATTCAGCAATGCGGATTCACCGTGCTGCTCATTTGGAAGAATTCGACCGGCTTTGACGTGTGTCCCTGCATCAGTACTGTGCAGTGACAGAAGCAAATACGTGTTTTGGGACGAGTACCATCCATCAGACAGTGCAAACGAGCTTATTGCCAAGGAACTCATAAAGAAATTCGGTTTGTCGCGCGTTGATGAAACGGATGCCCCTTCCCCCGCACCTGCTTTTGCTCCATCACCAGAGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

400

Amino Acids

43.61

Weight (kDa)

5.11

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 61 - 372 6.7e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74460
fragaria_vesca FvH4_5g14860
malus_domestica MD06G1194000.v1.1 MD14G1201200.v1.1
prunus_persica Prupe.5G194400_v2.0.a1
pyrus_communis pycom06g17190 pycom14g16670
rosa_chinensis RchiOBHm_Chr7g0181521
rosa_laevigata RLG00000005186
rosa_multiflora Rmu_sc0004621.1_g000032
rosa_roxburghii Rroxscaffold_3G00271930
rosa_rugosa Rorug06G0442000
rosa_samantha Rh7AG044100 Rh7BG043200 Rh7CG045000 Rh7DG043700
rosa_wichuraiana Rw7G003650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1180
Acc36I ACCTGC 2 cut(s) 288, 1180
AccBSI CCGCTC 1 cut(s) 772
AccII CGCG 2 cut(s) 1138, 1140
AciI CCGC 7 cut(s) 125, 359, 456, 551, 770, 968, 1167
AclWI GGATC 1 cut(s) 368
AcoI YGGCCR 3 cut(s) 126, 157, 310
AcsI RAATTY 3 cut(s) 557, 996, 1124
AdeI CACNNNGTG 1 cut(s) 15
AfaI GTAC 4 cut(s) 316, 790, 1032, 1073
AfiI CCNNNNNNNGG 2 cut(s) 263, 297
AflIII ACRYGT 2 cut(s) 1013, 1056
AgsI TTSAA 5 cut(s) 90, 404, 445, 506, 838
AjiI CACGTC 1 cut(s) 1014
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 9 cut(s) 107, 733, 827, 834, 845, 856, 880, 894, 1099
AluI AGCT 9 cut(s) 107, 733, 827, 834, 845, 856, 880, 894, 1099
Alw26I GTCTC 2 cut(s) 9, 242
AlwI GGATC 1 cut(s) 368
AlwNI CAGNNNCTG 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 79
AoxI GGCC 6 cut(s) 126, 142, 157, 310, 356, 363
ApeKI GCWGC 4 cut(s) 113, 548, 730, 981
ApoI RAATTY 3 cut(s) 557, 996, 1124
ArsI GACNNNNNNTTYG 2 cut(s) 19, 51
Asp700I GAANNNNTTC 1 cut(s) 560
AspA2I CCTAGG 1 cut(s) 718
AspLEI GCGC 2 cut(s) 542, 1140
AspS9I GGNCC 1 cut(s) 752
AsuHPI GGTGA 4 cut(s) 347, 917, 966, 1179
AvaI CYCGRG 1 cut(s) 79
AvaII GGWCC 1 cut(s) 752
AvrII CCTAGG 1 cut(s) 718
BalI TGGCCA 1 cut(s) 159
BanII GRGCYC 1 cut(s) 351
BbsI GAAGAC 2 cut(s) 29, 870
BbvI GCAGC 4 cut(s) 125, 560, 742, 968
BccI CCATC 6 cut(s) 49, 115, 149, 1083, 1087, 1192
BceAI ACGGC 2 cut(s) 113, 297
BcgI CGANNNNNNTGC 4 cut(s) 220, 254, 1085, 1119
BclI TGATCA 2 cut(s) 522, 933
BcoDI GTCTC 2 cut(s) 9, 242
BfaI CTAG 5 cut(s) 110, 689, 719, 749, 824
BfuAI ACCTGC 2 cut(s) 288, 1180
BisI GCNGC 7 cut(s) 114, 126, 359, 457, 549, 731, 982
BlnI CCTAGG 1 cut(s) 718
BlsI GCNGC 7 cut(s) 115, 127, 360, 458, 550, 732, 983
BmcAI AGTACT 1 cut(s) 1032
Bme18I GGWCC 1 cut(s) 752
BmeT110I CYCGRG 1 cut(s) 79
BmgBI CACGTC 1 cut(s) 1014
BmgT120I GGNCC 1 cut(s) 752
BmiI GGNNCC 1 cut(s) 350
BmsI GCATC 4 cut(s) 612, 898, 1034, 1144
BoxI GACNNNNGTC 1 cut(s) 1016
BpiI GAAGAC 2 cut(s) 29, 870
Bsa29I ATCGAT 2 cut(s) 495, 898
BsaAI YACGTR 1 cut(s) 1057
BsaJI CCNNGG 4 cut(s) 257, 353, 718, 1107
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 297
Bse118I RCCGGY 3 cut(s) 361, 452, 1003
Bse3DI GCAATG 3 cut(s) 123, 286, 970
BseCI ATCGAT 2 cut(s) 495, 898
BseDI CCNNGG 4 cut(s) 257, 353, 718, 1107
BseGI GGATG 2 cut(s) 1075, 1159
BseLI CCNNNNNNNGG 2 cut(s) 263, 297
BseMI GCAATG 3 cut(s) 123, 286, 970
BseMII CTCAG 1 cut(s) 844
BseRI GAGGAG 1 cut(s) 137
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 4 cut(s) 125, 560, 742, 968
BsgI GTGCAG 2 cut(s) 197, 1057
Bsh1236I CGCG 2 cut(s) 1138, 1140
Bsh1285I CGRYCG 2 cut(s) 129, 1004
BshFI GGCC 6 cut(s) 128, 144, 159, 312, 358, 365
BshVI ATCGAT 2 cut(s) 495, 898
BsiEI CGRYCG 2 cut(s) 129, 1004
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 3 cut(s) 362, 453, 1004
BslFI GGGAC 4 cut(s) 206, 523, 1004, 1079
BslI CCNNNNNNNGG 2 cut(s) 263, 297
BsmAI GTCTC 2 cut(s) 9, 242
BsmFI GGGAC 4 cut(s) 206, 523, 1004, 1079
BsnI GGCC 6 cut(s) 128, 144, 159, 312, 358, 365
BsoBI CYCGRG 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 6 cut(s) 373, 492, 496, 522, 655, 933
BspACI CCGC 7 cut(s) 125, 359, 456, 551, 770, 968, 1167
BspANI GGCC 6 cut(s) 128, 144, 159, 312, 358, 365
BspCNI CTCAG 1 cut(s) 843
BspDI ATCGAT 2 cut(s) 495, 898
BspFNI CGCG 2 cut(s) 1138, 1140
BspLI GGNNCC 1 cut(s) 350
BspMI ACCTGC 2 cut(s) 288, 1180
BspPI GGATC 1 cut(s) 368
BsrBI CCGCTC 1 cut(s) 772
BsrDI GCAATG 3 cut(s) 123, 286, 970
BsrFI RCCGGY 3 cut(s) 361, 452, 1003
BssAI RCCGGY 3 cut(s) 361, 452, 1003
BssECI CCNNGG 4 cut(s) 257, 353, 718, 1107
BssMI GATC 6 cut(s) 373, 492, 496, 522, 655, 933
BssT1I CCWWGG 4 cut(s) 257, 353, 718, 1107
Bst4CI ACNGT 5 cut(s) 319, 800, 978, 1035, 1088
Bst6I CTCTTC 1 cut(s) 143
BstBAI YACGTR 1 cut(s) 1057
BstC8I GCNNGC 6 cut(s) 250, 254, 363, 854, 878, 892
BstDEI CTNAG 2 cut(s) 785, 830
BstF5I GGATG 2 cut(s) 1075, 1159
BstFNI CGCG 2 cut(s) 1138, 1140
BstHHI GCGC 2 cut(s) 542, 1140
BstKTI GATC 6 cut(s) 376, 495, 499, 525, 658, 936
BstMAI GTCTC 2 cut(s) 9, 242
BstMBI GATC 6 cut(s) 373, 492, 496, 522, 655, 933
BstMCI CGRYCG 2 cut(s) 129, 1004
BstMWI GCNNNNNNNGC 6 cut(s) 113, 122, 165, 355, 487, 548
BstPAI GACNNNNGTC 1 cut(s) 1016
BstUI CGCG 2 cut(s) 1138, 1140
BstV1I GCAGC 4 cut(s) 125, 560, 742, 968
BstV2I GAAGAC 2 cut(s) 29, 870
BstZI CGGCCG 1 cut(s) 126
Bsu15I ATCGAT 2 cut(s) 495, 898
BsuRI GGCC 6 cut(s) 128, 144, 159, 312, 358, 365
BsuTUI ATCGAT 2 cut(s) 495, 898
BtrI CACGTC 1 cut(s) 1014
BtsCI GGATG 2 cut(s) 1075, 1159
BtsI GCAGTG 1 cut(s) 1045
BtsIMutI CAGTG 3 cut(s) 518, 1045, 1093
BveI ACCTGC 2 cut(s) 288, 1180
Cac8I GCNNGC 6 cut(s) 250, 254, 363, 854, 878, 892
CaiI CAGNNNCTG 1 cut(s) 323
CfoI GCGC 2 cut(s) 542, 1140
Cfr10I RCCGGY 3 cut(s) 361, 452, 1003
Cfr13I GGNCC 1 cut(s) 752
ClaI ATCGAT 2 cut(s) 495, 898
CseI GACGC 2 cut(s) 554, 578
Csp6I GTAC 4 cut(s) 315, 789, 1031, 1072
CviAII CATG 3 cut(s) 94, 343, 394
CviQI GTAC 4 cut(s) 315, 789, 1031, 1072
DdeI CTNAG 2 cut(s) 785, 830
DpnI GATC 6 cut(s) 375, 494, 498, 524, 657, 935
DpnII GATC 6 cut(s) 373, 492, 496, 522, 655, 933
DraIII CACNNNGTG 1 cut(s) 15
EaeI YGGCCR 3 cut(s) 126, 157, 310
EagI CGGCCG 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
EclXI CGGCCG 1 cut(s) 126
Eco130I CCWWGG 4 cut(s) 257, 353, 718, 1107
Eco24I GRGCYC 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 752
Eco52I CGGCCG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 79
EcoRI GAATTC 1 cut(s) 996
EcoT14I CCWWGG 4 cut(s) 257, 353, 718, 1107
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 4 cut(s) 257, 353, 718, 1107
FaeI CATG 3 cut(s) 97, 346, 397
FalI AAGNNNNNCTT 4 cut(s) 241, 273, 778, 810
FaqI GGGAC 4 cut(s) 206, 523, 1004, 1079
FatI CATG 3 cut(s) 93, 342, 393
FauI CCCGC 1 cut(s) 1174
FbaI TGATCA 2 cut(s) 522, 933
Fnu4HI GCNGC 7 cut(s) 114, 126, 359, 457, 549, 731, 982
FokI GGATG 2 cut(s) 1062, 1166
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 7 cut(s) 114, 126, 359, 457, 549, 731, 982
FspBI CTAG 5 cut(s) 110, 689, 719, 749, 824
GlaI GCGC 2 cut(s) 541, 1139
GluI GCNGC 7 cut(s) 114, 126, 359, 457, 549, 731, 982
HaeIII GGCC 6 cut(s) 128, 144, 159, 312, 358, 365
HapII CCGG 3 cut(s) 362, 453, 1004
HgaI GACGC 2 cut(s) 554, 578
HhaI GCGC 2 cut(s) 542, 1140
Hin1II CATG 3 cut(s) 97, 346, 397
Hin6I GCGC 2 cut(s) 540, 1138
HinP1I GCGC 2 cut(s) 540, 1138
HincII GTYRAC 1 cut(s) 925
HindII GTYRAC 1 cut(s) 925
HindIII AAGCTT 1 cut(s) 878
HinfI GANTC 6 cut(s) 133, 194, 235, 638, 957, 971
HpaII CCGG 3 cut(s) 362, 453, 1004
HphI GGTGA 4 cut(s) 347, 917, 966, 1179
Hpy166II GTNNAC 4 cut(s) 102, 415, 631, 925
Hpy188I TCNGA 3 cut(s) 205, 471, 1084
Hpy188III TCNNGA 4 cut(s) 59, 79, 371, 689
Hpy8I GTNNAC 4 cut(s) 102, 415, 631, 925
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 1 cut(s) 1170
HpyCH4III ACNGT 5 cut(s) 319, 800, 978, 1035, 1088
HpyCH4IV ACGT 3 cut(s) 207, 1013, 1056
HpyCH4V TGCA 7 cut(s) 72, 168, 178, 712, 1025, 1038, 1091
HpyF10VI GCNNNNNNNGC 6 cut(s) 113, 122, 165, 355, 487, 548
HpyF3I CTNAG 2 cut(s) 785, 830
HpySE526I ACGT 3 cut(s) 207, 1013, 1056
Hsp92II CATG 3 cut(s) 97, 346, 397
HspAI GCGC 2 cut(s) 540, 1138
KroI GCCGGC 1 cut(s) 361
KroNI GCCGGC 1 cut(s) 363
Ksp22I TGATCA 2 cut(s) 522, 933
Kzo9I GATC 6 cut(s) 373, 492, 496, 522, 655, 933
LmnI GCTCC 3 cut(s) 354, 777, 1186
Lsp1109I GCAGC 4 cut(s) 125, 560, 742, 968
LweI GCATC 4 cut(s) 612, 898, 1034, 1144
MaeI CTAG 5 cut(s) 110, 689, 719, 749, 824
MaeII ACGT 3 cut(s) 207, 1013, 1056
MaeIII GTNAC 3 cut(s) 130, 647, 1040
MalI GATC 6 cut(s) 375, 494, 498, 524, 657, 935
MbiI CCGCTC 1 cut(s) 772
MboI GATC 6 cut(s) 373, 492, 496, 522, 655, 933
MboII GAAGA 5 cut(s) 29, 130, 553, 875, 1005
MhlI GDGCHC 1 cut(s) 351
MlsI TGGCCA 1 cut(s) 159
MluCI AATT 4 cut(s) 534, 557, 996, 1124
MluNI TGGCCA 1 cut(s) 159
MlyI GAGTC 3 cut(s) 127, 188, 244
MmeI TCCRAC 2 cut(s) 190, 228
MnlI CCTC 8 cut(s) 155, 158, 209, 428, 433, 465, 720, 1186
Mox20I TGGCCA 1 cut(s) 159
MroNI GCCGGC 1 cut(s) 361
MroXI GAANNNNTTC 1 cut(s) 560
MscI TGGCCA 1 cut(s) 159
MseI TTAA 3 cut(s) 20, 609, 702
MslI CAYNNNNRTG 1 cut(s) 272
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 2 cut(s) 551, 733
MspI CCGG 3 cut(s) 362, 453, 1004
MvnI CGCG 2 cut(s) 1138, 1140
MwoI GCNNNNNNNGC 6 cut(s) 113, 122, 165, 355, 487, 548
NaeI GCCGGC 1 cut(s) 363
NdeII GATC 6 cut(s) 373, 492, 496, 522, 655, 933
NgoMIV GCCGGC 1 cut(s) 361
NlaIII CATG 3 cut(s) 97, 346, 397
NlaIV GGNNCC 1 cut(s) 350
NmuCI GTSAC 2 cut(s) 130, 1040
PaeR7I CTCGAG 1 cut(s) 79
PaqCI CACCTGC 1 cut(s) 1180
PdiI GCCGGC 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 560
PfeI GAWTC 3 cut(s) 638, 957, 971
PkrI GCNGC 7 cut(s) 115, 127, 360, 458, 550, 732, 983
PleI GAGTC 3 cut(s) 127, 188, 243
PpsI GAGTC 3 cut(s) 127, 188, 243
Ppu21I YACGTR 1 cut(s) 1057
PshAI GACNNNNGTC 1 cut(s) 1016
PspN4I GGNNCC 1 cut(s) 350
PspPI GGNCC 1 cut(s) 752
PstNI CAGNNNCTG 1 cut(s) 323
PvuII CAGCTG 1 cut(s) 733
RsaI GTAC 4 cut(s) 316, 790, 1032, 1073
RsaNI GTAC 4 cut(s) 315, 789, 1031, 1072
RseI CAYNNNNRTG 1 cut(s) 272
SaqAI TTAA 3 cut(s) 20, 609, 702
SatI GCNGC 7 cut(s) 114, 126, 359, 457, 549, 731, 982
Sau3AI GATC 6 cut(s) 373, 492, 496, 522, 655, 933
Sau96I GGNCC 1 cut(s) 752
ScaI AGTACT 1 cut(s) 1032
SchI GAGTC 3 cut(s) 127, 188, 244
SduI GDGCHC 1 cut(s) 351
SfaNI GCATC 4 cut(s) 612, 898, 1034, 1144
Sfr274I CTCGAG 1 cut(s) 79
SinI GGWCC 1 cut(s) 752
SlaI CTCGAG 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 79
SmoI CTYRAG 1 cut(s) 79
Sse9I AATT 4 cut(s) 534, 557, 996, 1124
SsiI CCGC 7 cut(s) 125, 359, 456, 551, 770, 968, 1167
SspMI CTAG 5 cut(s) 110, 689, 719, 749, 824
StyI CCWWGG 4 cut(s) 257, 353, 718, 1107
TaaI ACNGT 5 cut(s) 319, 800, 978, 1035, 1088
TaiI ACGT 3 cut(s) 210, 1016, 1059
TaqI TCGA 5 cut(s) 80, 495, 669, 898, 1000
TaqII GACCGA 1 cut(s) 401
TasI AATT 4 cut(s) 534, 557, 996, 1124
TatI WGTACW 1 cut(s) 1030
TauI GCSGC 3 cut(s) 128, 361, 459
TfiI GAWTC 3 cut(s) 638, 957, 971
Tru1I TTAA 3 cut(s) 20, 609, 702
Tru9I TTAA 3 cut(s) 20, 609, 702
TscAI CASTG 3 cut(s) 525, 1045, 1093
TseFI GTSAC 2 cut(s) 130, 1040
TseI GCWGC 4 cut(s) 113, 548, 730, 981
Tsp45I GTSAC 2 cut(s) 130, 1040
TspDTI ATGAA 5 cut(s) 110, 462, 595, 655, 1161
TspGWI ACGGA 2 cut(s) 366, 1166
TspRI CASTG 3 cut(s) 525, 1045, 1093
VpaK11BI GGWCC 1 cut(s) 752
XapI RAATTY 3 cut(s) 557, 996, 1124
XbaI TCTAGA 1 cut(s) 688
XhoI CTCGAG 1 cut(s) 79
XmaJI CCTAGG 1 cut(s) 718
XmnI GAANNNNTTC 1 cut(s) 560
XspI CTAG 5 cut(s) 110, 689, 719, 749, 824
ZrmI AGTACT 1 cut(s) 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.