pycom06g17640

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004478
Physical Location & Seq
Forward (+)
21554206 .. 21556749
2544 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g17640.5

Sequence Viewer

Length: 1641 bp
ATGGAATTCCGTCTCTCCTTCGTCCTCTTTCTGGTTTCTCTCCCTGCAATTTTAGCTCAAGATTTTGGGAAAACTAACAATGTTGAAAATGTTAAGGTTGTGGTTAACGGCATTTTAGCTGTTGCACGAGTTGACAATAACTTTATCTGTGCCACTATTGATTGGTGGAACCATGAAAAGTGTGACTACGGCCAGTGTCCATGGGGAGATGCATCCGCATTAAATTTGAACTTATCTCATCCTTTGCTAGCCAAGTCAATCCAAGCTTTCAATCAGTTGAGAATCAGAATTGGAGGTTCCTTGGAAGACCAACTGTTGTACGATGTTGGAAATTTGAAATATCCTTGCCATCCTTTCAGAAAGATGAAAGGTGGCTTGTTCGGTTTTTCTAGAGGATGTTTAACCATGAGTAGATGGGATGAGCTGAACCAATTTTTCAGCAAAACAAGGTCCATCGTCACATTTAGCTTGAATGCACTGTTGGGGAGGCACCACAAAGGCGGGGGAGTTTGGGTAGGAGACTGGGACTCTAGCAATGCTTATGATTTTATTAAGTACACCGTCTCAAAGAGATACCAGATAGATTCATGGGAATTTGGAAATGAGCTCAGTGGTAGTGGTGTTGAAGCTAGTGTTGGAGCTGGACAATACGGAAGAGATTTGATCAAGCTTAAACGCATCATCAACCATCTGTACAATGAGTCCGTTGCTAAACCTGCACTTATGGCGCCTGGAGGATTCTTTGACCAATCGTGGTTTGCCAGGCTTCTTCAGGTTTCTGGTTCTGGCACAGTCGATATCATCTCTCAGCATCTCTACAATTTGGGTGCAGGTGTTGATCCCATGTTGGTGAAAAAAATATTGGATCCCCATTACCTCGACCGTGCATATGGGTCATTCCATGATCTTGAACAAACTGTGAAAAAGAACGGTCCTTGGGCTTCTATATGGGTTGGTGAATCTGGAGGGGCCTATAACAGCGGTGGTCGTAATGTGTCTGACACATATGTGAACAGCTTTTGGTACTTGGATCAGCTCGGAATGTCTGCGAAGTACAATACCAGAGTATATTGCAGGCAGTCTCTAGTTGGCGGGAACTACGGTCTTCTCAACAGAACCTCATTTGTTCCTAACCCAGATTACTACAGTGCACTTCTATGGCATCGGCTTATGGGACGAGGAGTTCTTAGTGCCGACAACAATGGTTCAGCAGATTTACGCACTTATGCCCATTGTACAAGAGGAAGAGCGGGTATAACTCTGCTCCTTATCAATCTAAGCAATGAGACTAATTTCATTGTTAATATTGAAAATGTTTTGGACACCGATTTGGGTGCTCAAGCAAGAAACGCTGGCAAAGAGAGTAATTTCAAGCGCGGCCTGAAGAGAACGGTGTCATGGGTTGGACGCAAAGCATTAGACGAAGCAATTTACAGAGAAGAATACCATTTGACACCGAAAGATGGGGACGTCAAAAGCAAAACCATGGTTCACAACGGAGCTCCATTGGAGATTACCAAGGACGGAAACATCCCAGAATTGGAACCCTCTCGTGTTGACATGAAAGCTCCGTTAAGAATCTCCCCGTTGTCCATCAAGTTCGTGGTGTTACCTTTCTTTGATGCGCAAGCATGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko05205 map00531 map01100 map05205
Pfam Domains
Protein Families
Glyco_hydro_79n

Protein Analysis

547

Amino Acids

60.93

Weight (kDa)

8.79

Isoelectric Point (pI)

30.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 821
AatII GACGTC 1 cut(s) 1473
Acc16I TGCGCA 1 cut(s) 1626
Acc36I ACCTGC 2 cut(s) 724, 821
AccB1I GGYRCC 2 cut(s) 489, 727
AccBSI CCGCTC 1 cut(s) 1250
AccII CGCG 1 cut(s) 1377
AciI CCGC 6 cut(s) 216, 501, 981, 1092, 1250, 1377
AclWI GGATC 4 cut(s) 833, 860, 873, 1038
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 4 cut(s) 5, 223, 331, 593
AcuI CTGAAG 2 cut(s) 755, 1403
AcyI GRCGYC 2 cut(s) 728, 1470
AfaI GTAC 6 cut(s) 320, 557, 695, 1025, 1055, 1237
AfiI CCNNNNNNNGG 3 cut(s) 31, 1463, 1540
AgsI TTSAA 9 cut(s) 86, 229, 271, 337, 472, 626, 911, 1310, 1372
AjnI CCWGG 2 cut(s) 730, 761
AjuI GAANNNNNNNTTGG 6 cut(s) 464, 496, 618, 650, 845, 877
AleI CACNNNNGTG 1 cut(s) 1007
AloI GAACNNNNNNTCC 2 cut(s) 1167, 1199
Alw21I GWGCWC 4 cut(s) 609, 1153, 1339, 1504
Alw26I GTCTC 5 cut(s) 17, 513, 568, 1086, 1280
Alw44I GTGCAC 1 cut(s) 1149
AlwI GGATC 4 cut(s) 833, 860, 873, 1038
AoxI GGCC 3 cut(s) 190, 969, 1378
ApaLI GTGCAC 1 cut(s) 1149
ApoI RAATTY 4 cut(s) 5, 223, 331, 593
AspLEI GCGC 3 cut(s) 730, 1377, 1627
AspS9I GGNCC 3 cut(s) 450, 932, 969
AsuHPI GGTGA 2 cut(s) 862, 968
AsuNHI GCTAGC 1 cut(s) 247
AvaII GGWCC 2 cut(s) 450, 932
BaeGI GKGCMC 1 cut(s) 1153
BamHI GGATCC 1 cut(s) 865
BanI GGYRCC 2 cut(s) 489, 727
BanII GRGCYC 2 cut(s) 609, 1504
BauI CACGAG 2 cut(s) 126, 1551
BbsI GAAGAC 2 cut(s) 312, 1097
Bbv12I GWGCWC 4 cut(s) 609, 1153, 1339, 1504
BccI CCATC 6 cut(s) 357, 408, 461, 696, 1457, 1601
BceAI ACGGC 2 cut(s) 124, 205
BcgI CGANNNNNNTGC 2 cut(s) 1316, 1350
BciT130I CCWGG 2 cut(s) 732, 763
BclI TGATCA 1 cut(s) 663
BcoDI GTCTC 5 cut(s) 17, 513, 568, 1086, 1280
BfaI CTAG 5 cut(s) 248, 390, 531, 630, 1085
BfmI CTRYAG 1 cut(s) 1144
BfoI RGCGCY 1 cut(s) 731
BfuAI ACCTGC 2 cut(s) 724, 821
BisI GCNGC 1 cut(s) 1378
BlsI GCNGC 1 cut(s) 1379
Bme1390I CCNGG 2 cut(s) 732, 763
Bme18I GGWCC 2 cut(s) 450, 932
BmgT120I GGNCC 3 cut(s) 450, 932, 969
BmiI GGNNCC 7 cut(s) 170, 298, 491, 729, 867, 970, 1545
BmrFI CCNGG 2 cut(s) 732, 763
BmrI ACTGGG 1 cut(s) 532
BmsI GCATC 6 cut(s) 199, 221, 687, 820, 1171, 1612
BmtI GCTAGC 1 cut(s) 251
BmuI ACTGGG 1 cut(s) 532
BpiI GAAGAC 2 cut(s) 312, 1097
BpmI CTGGAG 2 cut(s) 753, 984
BpuEI CTTGAG 2 cut(s) 42, 1323
BsaHI GRCGYC 2 cut(s) 728, 1470
BsaJI CCNNGG 5 cut(s) 200, 300, 935, 1485, 1518
BsaXI ACNNNNNCTCC 2 cut(s) 498, 528
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 1463, 1540
Bse1I ACTGG 2 cut(s) 193, 527
Bse3DI GCAATG 2 cut(s) 541, 1288
BseBI CCWGG 2 cut(s) 732, 763
BseDI CCNNGG 5 cut(s) 200, 300, 935, 1485, 1518
BseGI GGATG 6 cut(s) 212, 238, 349, 401, 424, 1530
BseLI CCNNNNNNNGG 3 cut(s) 31, 1463, 1540
BseMI GCAATG 2 cut(s) 541, 1288
BseMII CTCAG 2 cut(s) 622, 821
BseNI ACTGG 2 cut(s) 193, 527
BseRI GAGGAG 1 cut(s) 1194
BseSI GKGCMC 1 cut(s) 1153
BsgI GTGCAG 2 cut(s) 702, 849
Bsh1236I CGCG 1 cut(s) 1377
Bsh1285I CGRYCG 1 cut(s) 883
BshFI GGCC 3 cut(s) 192, 971, 1380
BshNI GGYRCC 2 cut(s) 489, 727
BsiEI CGRYCG 1 cut(s) 883
BsiHKAI GWGCWC 4 cut(s) 609, 1153, 1339, 1504
BslFI GGGAC 3 cut(s) 539, 1188, 1481
BslI CCNNNNNNNGG 3 cut(s) 31, 1463, 1540
BsmAI GTCTC 5 cut(s) 17, 513, 568, 1086, 1280
BsmBI CGTCTC 2 cut(s) 17, 568
BsmFI GGGAC 3 cut(s) 539, 1188, 1481
BsmI GAATGC 1 cut(s) 478
BsnI GGCC 3 cut(s) 192, 971, 1380
Bsp1286I GDGCHC 4 cut(s) 609, 1153, 1339, 1504
Bsp1407I TGTACA 2 cut(s) 693, 1235
Bsp143I GATC 5 cut(s) 663, 838, 865, 904, 1030
Bsp19I CCATGG 2 cut(s) 200, 1485
BspACI CCGC 6 cut(s) 216, 501, 981, 1092, 1250, 1377
BspANI GGCC 3 cut(s) 192, 971, 1380
BspCNI CTCAG 2 cut(s) 621, 820
BspFNI CGCG 1 cut(s) 1377
BspLI GGNNCC 7 cut(s) 170, 298, 491, 729, 867, 970, 1545
BspMI ACCTGC 2 cut(s) 724, 821
BspOI GCTAGC 1 cut(s) 251
BspPI GGATC 4 cut(s) 833, 860, 873, 1038
BspQI GCTCTTC 1 cut(s) 1240
BspT107I GGYRCC 2 cut(s) 489, 727
BsrBI CCGCTC 1 cut(s) 1250
BsrDI GCAATG 2 cut(s) 541, 1288
BsrGI TGTACA 2 cut(s) 693, 1235
BsrI ACTGG 2 cut(s) 193, 527
BssECI CCNNGG 5 cut(s) 200, 300, 935, 1485, 1518
BssMI GATC 5 cut(s) 663, 838, 865, 904, 1030
BssNI GRCGYC 2 cut(s) 728, 1470
BssSI CACGAG 2 cut(s) 126, 1551
BssT1I CCWWGG 5 cut(s) 200, 300, 935, 1485, 1518
Bst2BI CACGAG 2 cut(s) 126, 1551
Bst2UI CCWGG 2 cut(s) 732, 763
Bst6I CTCTTC 3 cut(s) 649, 1240, 1379
BstACI GRCGYC 2 cut(s) 728, 1470
BstAUI TGTACA 2 cut(s) 693, 1235
BstC8I GCNNGC 4 cut(s) 249, 1076, 1354, 1629
BstDEI CTNAG 4 cut(s) 608, 807, 1187, 1277
BstDSI CCRYGG 2 cut(s) 200, 1485
BstF5I GGATG 6 cut(s) 212, 238, 349, 401, 424, 1530
BstFNI CGCG 1 cut(s) 1377
BstH2I RGCGCY 1 cut(s) 731
BstHHI GCGC 3 cut(s) 730, 1377, 1627
BstKTI GATC 5 cut(s) 666, 841, 868, 907, 1033
BstMAI GTCTC 5 cut(s) 17, 513, 568, 1086, 1280
BstMBI GATC 5 cut(s) 663, 838, 865, 904, 1030
BstMCI CGRYCG 1 cut(s) 883
BstMWI GCNNNNNNNGC 4 cut(s) 53, 716, 725, 1349
BstNI CCWGG 2 cut(s) 732, 763
BstNSI RCATGY 1 cut(s) 1635
BstSCI CCNGG 2 cut(s) 730, 761
BstSFI CTRYAG 1 cut(s) 1144
BstSLI GKGCMC 1 cut(s) 1153
BstUI CGCG 1 cut(s) 1377
BstV2I GAAGAC 2 cut(s) 312, 1097
BstX2I RGATCY 1 cut(s) 865
BstYI RGATCY 1 cut(s) 865
BsuRI GGCC 3 cut(s) 192, 971, 1380
BtgI CCRYGG 2 cut(s) 200, 1485
BtsCI GGATG 6 cut(s) 212, 238, 349, 401, 424, 1530
BtsIMutI CAGTG 4 cut(s) 200, 476, 616, 1153
BveI ACCTGC 2 cut(s) 724, 821
Cac8I GCNNGC 4 cut(s) 249, 1076, 1354, 1629
CfoI GCGC 3 cut(s) 730, 1377, 1627
Cfr13I GGNCC 3 cut(s) 450, 932, 969
CseI GACGC 1 cut(s) 1416
Csp6I GTAC 6 cut(s) 319, 556, 694, 1024, 1054, 1236
CviQI GTAC 6 cut(s) 319, 556, 694, 1024, 1054, 1236
DdeI CTNAG 4 cut(s) 608, 807, 1187, 1277
DinI GGCGCC 1 cut(s) 729
DpnI GATC 5 cut(s) 665, 840, 867, 906, 1032
DpnII GATC 5 cut(s) 663, 838, 865, 904, 1030
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 3 cut(s) 649, 1240, 1379
EarI CTCTTC 3 cut(s) 649, 1240, 1379
Ecl136II GAGCTC 2 cut(s) 607, 1502
Eco130I CCWWGG 5 cut(s) 200, 300, 935, 1485, 1518
Eco24I GRGCYC 2 cut(s) 609, 1504
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 2 cut(s) 450, 932
Eco53kI GAGCTC 2 cut(s) 607, 1502
Eco57I CTGAAG 2 cut(s) 755, 1403
EcoICRI GAGCTC 2 cut(s) 607, 1502
EcoO109I RGGNCCY 1 cut(s) 969
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 2 cut(s) 730, 761
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 5 cut(s) 200, 300, 935, 1485, 1518
EcoT22I ATGCAT 1 cut(s) 214
EcoT38I GRGCYC 2 cut(s) 609, 1504
EgeI GGCGCC 1 cut(s) 729
EheI GGCGCC 1 cut(s) 729
ErhI CCWWGG 5 cut(s) 200, 300, 935, 1485, 1518
Esp3I CGTCTC 2 cut(s) 17, 568
FaqI GGGAC 3 cut(s) 539, 1188, 1481
FauI CCCGC 3 cut(s) 494, 1085, 1243
FauNDI CATATG 2 cut(s) 889, 1006
FbaI TGATCA 1 cut(s) 663
Fnu4HI GCNGC 1 cut(s) 1378
FokI GGATG 6 cut(s) 199, 225, 336, 408, 431, 1517
FriOI GRGCYC 2 cut(s) 609, 1504
Fsp4HI GCNGC 1 cut(s) 1378
FspBI CTAG 5 cut(s) 248, 390, 531, 630, 1085
FspI TGCGCA 1 cut(s) 1626
GlaI GCGC 3 cut(s) 729, 1376, 1626
GluI GCNGC 1 cut(s) 1378
GsuI CTGGAG 2 cut(s) 753, 984
HaeII RGCGCY 1 cut(s) 731
HaeIII GGCC 3 cut(s) 192, 971, 1380
HgaI GACGC 1 cut(s) 1416
HhaI GCGC 3 cut(s) 730, 1377, 1627
Hin1I GRCGYC 2 cut(s) 728, 1470
Hin6I GCGC 3 cut(s) 728, 1375, 1625
HinP1I GCGC 3 cut(s) 728, 1375, 1625
HincII GTYRAC 3 cut(s) 106, 133, 1558
HindII GTYRAC 3 cut(s) 106, 133, 1558
HindIII AAGCTT 2 cut(s) 264, 668
HinfI GANTC 7 cut(s) 282, 527, 584, 701, 738, 959, 1578
HpaI GTTAAC 1 cut(s) 106
HphI GGTGA 2 cut(s) 862, 968
Hpy166II GTNNAC 7 cut(s) 106, 133, 558, 1012, 1151, 1492, 1558
Hpy188I TCNGA 4 cut(s) 287, 359, 1000, 1040
Hpy188III TCNNGA 4 cut(s) 59, 390, 908, 963
Hpy8I GTNNAC 7 cut(s) 106, 133, 558, 1012, 1151, 1492, 1558
HpyAV CCTTC 1 cut(s) 28
HpyCH4IV ACGT 1 cut(s) 1470
HpyCH4V TGCA 9 cut(s) 47, 125, 212, 476, 719, 830, 887, 1074, 1151
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 716, 725, 1349
HpyF3I CTNAG 4 cut(s) 608, 807, 1187, 1277
HpySE526I ACGT 1 cut(s) 1470
Hsp92I GRCGYC 2 cut(s) 728, 1470
HspAI GCGC 3 cut(s) 728, 1375, 1625
KasI GGCGCC 1 cut(s) 727
Ksp22I TGATCA 1 cut(s) 663
KspAI GTTAAC 1 cut(s) 106
Kzo9I GATC 5 cut(s) 663, 838, 865, 904, 1030
LguI GCTCTTC 1 cut(s) 1240
LmnI GCTCC 5 cut(s) 638, 1269, 1499, 1507, 1573
LweI GCATC 6 cut(s) 199, 221, 687, 820, 1171, 1612
MaeI CTAG 5 cut(s) 248, 390, 531, 630, 1085
MaeII ACGT 1 cut(s) 1470
MaeIII GTNAC 3 cut(s) 182, 457, 1608
MalI GATC 5 cut(s) 665, 840, 867, 906, 1032
MbiI CCGCTC 1 cut(s) 1250
MboI GATC 5 cut(s) 663, 838, 865, 904, 1030
MboII GAAGA 7 cut(s) 317, 666, 761, 1097, 1257, 1396, 1451
MflI RGATCY 1 cut(s) 865
MhlI GDGCHC 4 cut(s) 609, 1153, 1339, 1504
Mly113I GGCGCC 1 cut(s) 728
MlyI GAGTC 2 cut(s) 521, 710
MmeI TCCRAC 3 cut(s) 307, 616, 1384
Mph1103I ATGCAT 1 cut(s) 214
MseI TTAA 8 cut(s) 93, 105, 221, 401, 552, 672, 1302, 1574
MslI CAYNNNNRTG 4 cut(s) 848, 1007, 1156, 1636
MspA1I CMGCKG 1 cut(s) 981
MspR9I CCNGG 2 cut(s) 732, 763
Mva1269I GAATGC 1 cut(s) 478
MvaI CCWGG 2 cut(s) 732, 763
MvnI CGCG 1 cut(s) 1377
MwoI GCNNNNNNNGC 4 cut(s) 53, 716, 725, 1349
NarI GGCGCC 1 cut(s) 728
NcoI CCATGG 2 cut(s) 200, 1485
NdeI CATATG 2 cut(s) 889, 1006
NdeII GATC 5 cut(s) 663, 838, 865, 904, 1030
NheI GCTAGC 1 cut(s) 247
NlaIV GGNNCC 7 cut(s) 170, 298, 491, 729, 867, 970, 1545
NmuCI GTSAC 2 cut(s) 182, 457
NsbI TGCGCA 1 cut(s) 1626
NsiI ATGCAT 1 cut(s) 214
NspI RCATGY 1 cut(s) 1635
OliI CACNNNNGTG 1 cut(s) 1007
PaqCI CACCTGC 1 cut(s) 821
PciSI GCTCTTC 1 cut(s) 1240
PctI GAATGC 1 cut(s) 478
PfeI GAWTC 5 cut(s) 282, 584, 738, 959, 1578
PkrI GCNGC 1 cut(s) 1379
PleI GAGTC 2 cut(s) 521, 709
PluTI GGCGCC 1 cut(s) 731
PpsI GAGTC 2 cut(s) 521, 709
Psp124BI GAGCTC 2 cut(s) 609, 1504
Psp6I CCWGG 2 cut(s) 730, 761
PspGI CCWGG 2 cut(s) 730, 761
PspN4I GGNNCC 7 cut(s) 170, 298, 491, 729, 867, 970, 1545
PspPI GGNCC 3 cut(s) 450, 932, 969
PsuI RGATCY 1 cut(s) 865
RsaI GTAC 6 cut(s) 320, 557, 695, 1025, 1055, 1237
RsaNI GTAC 6 cut(s) 319, 556, 694, 1024, 1054, 1236
RseI CAYNNNNRTG 4 cut(s) 848, 1007, 1156, 1636
SacI GAGCTC 2 cut(s) 609, 1504
SapI GCTCTTC 1 cut(s) 1240
SaqAI TTAA 8 cut(s) 93, 105, 221, 401, 552, 672, 1302, 1574
SatI GCNGC 1 cut(s) 1378
Sau3AI GATC 5 cut(s) 663, 838, 865, 904, 1030
Sau96I GGNCC 3 cut(s) 450, 932, 969
SchI GAGTC 2 cut(s) 521, 710
ScrFI CCNGG 2 cut(s) 732, 763
SduI GDGCHC 4 cut(s) 609, 1153, 1339, 1504
SfaNI GCATC 6 cut(s) 199, 221, 687, 820, 1171, 1612
SfcI CTRYAG 1 cut(s) 1144
SfoI GGCGCC 1 cut(s) 729
SinI GGWCC 2 cut(s) 450, 932
SmiMI CAYNNNNRTG 4 cut(s) 848, 1007, 1156, 1636
SmlI CTYRAG 2 cut(s) 57, 1338
SmoI CTYRAG 2 cut(s) 57, 1338
SsiI CCGC 6 cut(s) 216, 501, 981, 1092, 1250, 1377
SspDI GGCGCC 1 cut(s) 727
SspI AATATT 2 cut(s) 861, 1306
SspMI CTAG 5 cut(s) 248, 390, 531, 630, 1085
SstI GAGCTC 2 cut(s) 609, 1504
StyD4I CCNGG 2 cut(s) 730, 761
StyI CCWWGG 5 cut(s) 200, 300, 935, 1485, 1518
TaiI ACGT 1 cut(s) 1473
TaqI TCGA 2 cut(s) 795, 879
TatI WGTACW 4 cut(s) 555, 693, 1053, 1235
TauI GCSGC 1 cut(s) 1380
TfiI GAWTC 5 cut(s) 282, 584, 738, 959, 1578
Tru1I TTAA 8 cut(s) 93, 105, 221, 401, 552, 672, 1302, 1574
Tru9I TTAA 8 cut(s) 93, 105, 221, 401, 552, 672, 1302, 1574
TscAI CASTG 4 cut(s) 200, 483, 616, 1153
TseFI GTSAC 2 cut(s) 182, 457
Tsp45I GTSAC 2 cut(s) 182, 457
TspDTI ATGAA 5 cut(s) 189, 380, 576, 1285, 1577
TspGWI ACGGA 5 cut(s) 666, 694, 1512, 1539, 1560
TspRI CASTG 4 cut(s) 200, 483, 616, 1153
VneI GTGCAC 1 cut(s) 1149
VpaK11BI GGWCC 2 cut(s) 450, 932
XapI RAATTY 4 cut(s) 5, 223, 331, 593
XbaI TCTAGA 1 cut(s) 389
XceI RCATGY 1 cut(s) 1635
XcmI CCANNNNNNNNNTGG 1 cut(s) 1600
XspI CTAG 5 cut(s) 248, 390, 531, 630, 1085
ZraI GACGTC 1 cut(s) 1471
Zsp2I ATGCAT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.