Rorug06G0448300

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004478
Physical Location & Seq
Reverse (-)
57841277 .. 57843136
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0448300.1

Sequence Viewer

Length: 1029 bp
ATGGCGGTGGAGATGATGAGCTTTCCGAAGATGGAAGACCAAAAGGCCATCCAGGAGGCCGCATCGCAAGGCTTGAAGAGCATGGAGCACCTGATTCGTTACCTCTCTCACCAGCAACAGTCTAACCAGCCCTCCTCTCGCGTGGACTGCACCGACCTCACAGACCACACCGTCTCCAAGTTCAAAAAGGTCATTTCCCTTCTGAACCGGACCGGCCACGCCCGGTTCAGGCGCGGACCGGTCGCCGCGGTTCCGGCTCCTCATCATCATCAGTTCTATTCTTCTTCTTCTTCTTCTTCTCCGCCGTCCACCTCTCTCCTCTTCCCTCCCTCCCAGACCTTGAGCTTGGCTCCGGCACCAGTCGCTCCCCCGGCGATTGCTCCTCCGGCTCCGGTCAAGGCTCCGGAGACTAATTTTGCTCATTCCATGACGCTTGACTTCACCAGGCCTAATGCTCTGGCCTCAAACCCTAAGTGTACCGACATCGAGTTCAGCGTCTCGTCGAGCTCGTCGTTTATGTCGTCGGCCATCACCGGAGACAATAGCGTCTCCAACGGCAAGCTCGGTCTGTTCATTGCTCCCAATCCGGCCCCGGCCGTCTCCGGCGCCAAGCCTCCGCTCTCCGCCGCGCCGTTTAAGAAGCGGTGCCACGAGCACGACCACTCCGACGATGTCTCCTGCAAGTTATCCGGATCAGGATCCGCTTCCGGGTCGGGCAAGTGCCACTGCTCCAAGAGAAGGAAAAATCGGGTGAAGAAAACAATCCGAGTCCCGGCGATTAGTTCAAAGATCGCCGATATTCCGCCGGACGAGTACTCCTGGAGAAAGTACGGCCAGAAGCCGATCAAAGGCTCACCTTACCCAAGGGGTTATTACAAGTGTAGTACAGTGAGGGGGTGTCCGGCAAGAAAACACGTGGAGCGAGCACCGGACGATCCGGCGATGCTGATCGTAACGTACGAGGGGGAGCATCGACACGCTCCGGATAATATGAGTGGCGGAGTTGGGATGGTATTCGAGTCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko05205 map00531 map01100 map05205
Pfam Domains
Protein Families
Glyco_hydro_79n

Protein Analysis

342

Amino Acids

36.87

Weight (kDa)

9.51

Isoelectric Point (pI)

56.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_zn_clust PF10533 213 - 266 1.2e-19 Plant zinc cluster domain
WRKY PF03106 270 - 326 8.2e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 170, 545
AccB1I GGYRCC 3 cut(s) 355, 605, 645
AccBSI CCGCTC 1 cut(s) 619
AccII CGCG 4 cut(s) 141, 234, 248, 629
AccIII TCCGGA 3 cut(s) 403, 689, 982
AclWI GGATC 4 cut(s) 693, 700, 706, 929
AcoI YGGCCR 4 cut(s) 214, 525, 594, 832
AcvI CACGTG 1 cut(s) 916
AcyI GRCGYC 1 cut(s) 606
AfaI GTAC 5 cut(s) 478, 815, 830, 886, 959
AfiI CCNNNNNNNGG 5 cut(s) 228, 708, 738, 772, 848
AflIII ACRYGT 1 cut(s) 913
AgeI ACCGGT 1 cut(s) 238
AgsI TTSAA 3 cut(s) 76, 184, 786
AjnI CCWGG 3 cut(s) 51, 443, 818
AluBI AGCT 4 cut(s) 21, 345, 507, 562
AluI AGCT 4 cut(s) 21, 345, 507, 562
Alw21I GWGCWC 4 cut(s) 90, 509, 657, 928
Alw26I GTCTC 7 cut(s) 178, 401, 502, 531, 553, 604, 679
AlwI GGATC 4 cut(s) 693, 700, 706, 929
Aor13HI TCCGGA 3 cut(s) 403, 689, 982
AoxI GGCC 9 cut(s) 45, 57, 214, 446, 459, 525, 588, 594, 832
AsiGI ACCGGT 1 cut(s) 238
AspLEI GCGC 3 cut(s) 234, 608, 631
AspS9I GGNCC 3 cut(s) 210, 236, 589
AsuC2I CCSGG 5 cut(s) 223, 371, 593, 709, 773
AsuHPI GGTGA 5 cut(s) 101, 433, 523, 763, 846
AvaII GGWCC 2 cut(s) 210, 236
BamHI GGATCC 1 cut(s) 698
BanI GGYRCC 3 cut(s) 355, 605, 645
BanII GRGCYC 1 cut(s) 509
BauI CACGAG 1 cut(s) 650
BbrPI CACGTG 1 cut(s) 916
BbsI GAAGAC 1 cut(s) 42
Bbv12I GWGCWC 4 cut(s) 90, 509, 657, 928
BccI CCATC 4 cut(s) 25, 56, 536, 1003
BceAI ACGGC 5 cut(s) 289, 571, 581, 616, 847
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 3 cut(s) 53, 445, 820
BcnI CCSGG 5 cut(s) 223, 371, 593, 709, 773
BcoDI GTCTC 7 cut(s) 178, 401, 502, 531, 553, 604, 679
BfoI RGCGCY 1 cut(s) 609
BisI GCNGC 3 cut(s) 60, 246, 627
BlsI GCNGC 3 cut(s) 61, 247, 628
BmcAI AGTACT 1 cut(s) 815
Bme1390I CCNGG 8 cut(s) 53, 223, 371, 445, 593, 709, 773, 820
Bme18I GGWCC 2 cut(s) 210, 236
BmgT120I GGNCC 3 cut(s) 210, 236, 589
BmrFI CCNGG 8 cut(s) 53, 223, 371, 445, 593, 709, 773, 820
BmsI GCATC 3 cut(s) 71, 933, 979
BpiI GAAGAC 1 cut(s) 42
BplI GAGNNNNNCTC 2 cut(s) 334, 366
BpmI CTGGAG 1 cut(s) 841
BpuEI CTTGAG 1 cut(s) 361
BpuMI CCSGG 5 cut(s) 223, 371, 593, 709, 773
BsaAI YACGTR 1 cut(s) 916
BsaBI GATNNNNATC 2 cut(s) 697, 947
BsaHI GRCGYC 1 cut(s) 606
BsaJI CCNNGG 4 cut(s) 246, 369, 591, 863
BsaWI WCCGGW 8 cut(s) 207, 238, 391, 403, 533, 689, 928, 982
Bsc4I CCNNNNNNNGG 5 cut(s) 228, 708, 738, 772, 848
Bse118I RCCGGY 2 cut(s) 212, 238
Bse1I ACTGG 1 cut(s) 359
Bse3DI GCAATG 1 cut(s) 573
Bse8I GATNNNNATC 2 cut(s) 697, 947
BseAI TCCGGA 3 cut(s) 403, 689, 982
BseBI CCWGG 3 cut(s) 53, 445, 820
BseDI CCNNGG 4 cut(s) 246, 369, 591, 863
BseGI GGATG 2 cut(s) 48, 1014
BseJI GATNNNNATC 2 cut(s) 697, 947
BseLI CCNNNNNNNGG 5 cut(s) 228, 708, 738, 772, 848
BseMI GCAATG 1 cut(s) 573
BseNI ACTGG 1 cut(s) 359
BseRI GAGGAG 4 cut(s) 124, 249, 308, 372
BseX3I CGGCCG 1 cut(s) 594
BsgI GTGCAG 1 cut(s) 133
Bsh1236I CGCG 4 cut(s) 141, 234, 248, 629
Bsh1285I CGRYCG 2 cut(s) 243, 597
BshFI GGCC 9 cut(s) 47, 59, 216, 448, 461, 527, 590, 596, 834
BshNI GGYRCC 3 cut(s) 355, 605, 645
BshTI ACCGGT 1 cut(s) 238
BsiEI CGRYCG 2 cut(s) 243, 597
BsiHKAI GWGCWC 4 cut(s) 90, 509, 657, 928
BsiWI CGTACG 1 cut(s) 957
BslFI GGGAC 1 cut(s) 755
BslI CCNNNNNNNGG 5 cut(s) 228, 708, 738, 772, 848
BsmAI GTCTC 7 cut(s) 178, 401, 502, 531, 553, 604, 679
BsmBI CGTCTC 4 cut(s) 178, 502, 553, 604
BsmFI GGGAC 1 cut(s) 755
BsnI GGCC 9 cut(s) 47, 59, 216, 448, 461, 527, 590, 596, 834
Bsp1286I GDGCHC 4 cut(s) 90, 509, 657, 928
Bsp13I TCCGGA 3 cut(s) 403, 689, 982
Bsp143I GATC 6 cut(s) 692, 698, 789, 843, 934, 948
BspANI GGCC 9 cut(s) 47, 59, 216, 448, 461, 527, 590, 596, 834
BspEI TCCGGA 3 cut(s) 403, 689, 982
BspFNI CGCG 4 cut(s) 141, 234, 248, 629
BspPI GGATC 4 cut(s) 693, 700, 706, 929
BspQI GCTCTTC 1 cut(s) 71
BspT107I GGYRCC 3 cut(s) 355, 605, 645
BsrBI CCGCTC 1 cut(s) 619
BsrDI GCAATG 1 cut(s) 573
BsrFI RCCGGY 2 cut(s) 212, 238
BsrI ACTGG 1 cut(s) 359
BssAI RCCGGY 2 cut(s) 212, 238
BssECI CCNNGG 4 cut(s) 246, 369, 591, 863
BssMI GATC 6 cut(s) 692, 698, 789, 843, 934, 948
BssNI GRCGYC 1 cut(s) 606
BssSI CACGAG 1 cut(s) 650
BssT1I CCWWGG 1 cut(s) 863
Bst2BI CACGAG 1 cut(s) 650
Bst2UI CCWGG 3 cut(s) 53, 445, 820
Bst4CI ACNGT 3 cut(s) 120, 172, 889
Bst6I CTCTTC 2 cut(s) 71, 326
BstACI GRCGYC 1 cut(s) 606
BstBAI YACGTR 1 cut(s) 916
BstC8I GCNNGC 2 cut(s) 560, 924
BstDEI CTNAG 1 cut(s) 471
BstDSI CCRYGG 1 cut(s) 246
BstF5I GGATG 2 cut(s) 48, 1014
BstFNI CGCG 4 cut(s) 141, 234, 248, 629
BstH2I RGCGCY 1 cut(s) 609
BstHHI GCGC 3 cut(s) 234, 608, 631
BstKTI GATC 6 cut(s) 695, 701, 792, 846, 937, 951
BstMAI GTCTC 7 cut(s) 178, 401, 502, 531, 553, 604, 679
BstMBI GATC 6 cut(s) 692, 698, 789, 843, 934, 948
BstMCI CGRYCG 2 cut(s) 243, 597
BstMWI GCNNNNNNNGC 6 cut(s) 78, 147, 254, 362, 371, 386
BstNI CCWGG 3 cut(s) 53, 445, 820
BstSCI CCNGG 8 cut(s) 51, 221, 369, 443, 591, 707, 771, 818
BstUI CGCG 4 cut(s) 141, 234, 248, 629
BstV2I GAAGAC 1 cut(s) 42
BstX2I RGATCY 1 cut(s) 698
BstYI RGATCY 1 cut(s) 698
BstZI CGGCCG 1 cut(s) 594
BsuRI GGCC 9 cut(s) 47, 59, 216, 448, 461, 527, 590, 596, 834
BtgI CCRYGG 1 cut(s) 246
BtgZI GCGATG 2 cut(s) 48, 956
BtsCI GGATG 2 cut(s) 48, 1014
BtsI GCAGTG 1 cut(s) 724
BtsIMutI CAGTG 2 cut(s) 724, 894
Cac8I GCNNGC 2 cut(s) 560, 924
CfoI GCGC 3 cut(s) 234, 608, 631
Cfr10I RCCGGY 2 cut(s) 212, 238
Cfr13I GGNCC 3 cut(s) 210, 236, 589
Cfr42I CCGCGG 1 cut(s) 249
CpoI CGGWCCG 2 cut(s) 210, 236
CseI GACGC 3 cut(s) 439, 484, 535
Csp6I GTAC 5 cut(s) 477, 814, 829, 885, 958
CspAI ACCGGT 1 cut(s) 238
CspI CGGWCCG 2 cut(s) 210, 236
CviAII CATG 2 cut(s) 82, 427
CviQI GTAC 5 cut(s) 477, 814, 829, 885, 958
DdeI CTNAG 1 cut(s) 471
DinI GGCGCC 1 cut(s) 607
DpnI GATC 6 cut(s) 694, 700, 791, 845, 936, 950
DpnII GATC 6 cut(s) 692, 698, 789, 843, 934, 948
DrdI GACNNNNNNGTC 2 cut(s) 170, 545
DseDI GACNNNNNNGTC 2 cut(s) 170, 545
EaeI YGGCCR 4 cut(s) 214, 525, 594, 832
EagI CGGCCG 1 cut(s) 594
Eam1104I CTCTTC 2 cut(s) 71, 326
EarI CTCTTC 2 cut(s) 71, 326
EciI GGCGGA 4 cut(s) 291, 613, 792, 1014
Ecl136II GAGCTC 1 cut(s) 507
EclXI CGGCCG 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 863
Eco147I AGGCCT 1 cut(s) 448
Eco24I GRGCYC 1 cut(s) 509
Eco47I GGWCC 2 cut(s) 210, 236
Eco52I CGGCCG 1 cut(s) 594
Eco53kI GAGCTC 1 cut(s) 507
Eco72I CACGTG 1 cut(s) 916
EcoICRI GAGCTC 1 cut(s) 507
EcoRII CCWGG 3 cut(s) 51, 443, 818
EcoT14I CCWWGG 1 cut(s) 863
EcoT38I GRGCYC 1 cut(s) 509
EgeI GGCGCC 1 cut(s) 607
EheI GGCGCC 1 cut(s) 607
ErhI CCWWGG 1 cut(s) 863
Esp3I CGTCTC 4 cut(s) 178, 502, 553, 604
FaeI CATG 2 cut(s) 85, 430
FaiI YATR 4 cut(s) 83, 428, 518, 992
FaqI GGGAC 1 cut(s) 755
FatI CATG 2 cut(s) 81, 426
Fnu4HI GCNGC 3 cut(s) 60, 246, 627
FokI GGATG 2 cut(s) 35, 1021
FriOI GRGCYC 1 cut(s) 509
Fsp4HI GCNGC 3 cut(s) 60, 246, 627
GlaI GCGC 3 cut(s) 233, 607, 630
GluI GCNGC 3 cut(s) 60, 246, 627
GsuI CTGGAG 1 cut(s) 841
HaeII RGCGCY 1 cut(s) 609
HaeIII GGCC 9 cut(s) 47, 59, 216, 448, 461, 527, 590, 596, 834
HgaI GACGC 3 cut(s) 439, 484, 535
HhaI GCGC 3 cut(s) 234, 608, 631
Hin1I GRCGYC 1 cut(s) 606
Hin1II CATG 2 cut(s) 85, 430
Hin6I GCGC 3 cut(s) 232, 606, 629
HinP1I GCGC 3 cut(s) 232, 606, 629
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HinfI GANTC 3 cut(s) 94, 768, 1019
HphI GGTGA 5 cut(s) 101, 433, 523, 763, 846
Hpy166II GTNNAC 4 cut(s) 145, 309, 477, 1023
Hpy188I TCNGA 4 cut(s) 27, 204, 667, 767
Hpy188III TCNNGA 4 cut(s) 404, 690, 696, 983
Hpy8I GTNNAC 4 cut(s) 145, 309, 477, 1023
Hpy99I CGWCG 4 cut(s) 505, 514, 526, 671
HpyAV CCTTC 2 cut(s) 209, 732
HpyCH4III ACNGT 3 cut(s) 120, 172, 889
HpyCH4IV ACGT 2 cut(s) 915, 956
HpyCH4V TGCA 2 cut(s) 150, 681
HpyF10VI GCNNNNNNNGC 6 cut(s) 78, 147, 254, 362, 371, 386
HpyF3I CTNAG 1 cut(s) 471
HpySE526I ACGT 2 cut(s) 915, 956
Hsp92I GRCGYC 1 cut(s) 606
Hsp92II CATG 2 cut(s) 85, 430
HspAI GCGC 3 cut(s) 232, 606, 629
KasI GGCGCC 1 cut(s) 605
Kpn2I TCCGGA 3 cut(s) 403, 689, 982
KspI CCGCGG 1 cut(s) 249
Kzo9I GATC 6 cut(s) 692, 698, 789, 843, 934, 948
LguI GCTCTTC 1 cut(s) 71
LweI GCATC 3 cut(s) 71, 933, 979
MaeII ACGT 2 cut(s) 915, 956
MaeIII GTNAC 2 cut(s) 98, 952
MalI GATC 6 cut(s) 694, 700, 791, 845, 936, 950
MbiI CCGCTC 1 cut(s) 619
MboI GATC 6 cut(s) 692, 698, 789, 843, 934, 948
MflI RGATCY 1 cut(s) 698
MhlI GDGCHC 4 cut(s) 90, 509, 657, 928
MluCI AATT 1 cut(s) 412
Mly113I GGCGCC 1 cut(s) 606
MlyI GAGTC 2 cut(s) 777, 1028
MmeI TCCRAC 2 cut(s) 576, 690
MroI TCCGGA 3 cut(s) 403, 689, 982
MseI TTAA 1 cut(s) 636
MspA1I CMGCKG 1 cut(s) 248
MspR9I CCNGG 8 cut(s) 53, 223, 371, 445, 593, 709, 773, 820
MvaI CCWGG 3 cut(s) 53, 445, 820
MvnI CGCG 4 cut(s) 141, 234, 248, 629
MwoI GCNNNNNNNGC 6 cut(s) 78, 147, 254, 362, 371, 386
NarI GGCGCC 1 cut(s) 606
NciI CCSGG 5 cut(s) 223, 371, 593, 709, 773
NdeII GATC 6 cut(s) 692, 698, 789, 843, 934, 948
NlaIII CATG 2 cut(s) 85, 430
PceI AGGCCT 1 cut(s) 448
PciSI GCTCTTC 1 cut(s) 71
PcsI WCGNNNNNNNCGW 6 cut(s) 492, 506, 509, 518, 663, 957
PfeI GAWTC 1 cut(s) 94
Pfl23II CGTACG 1 cut(s) 957
PflFI GACNNNGTC 1 cut(s) 671
PfoI TCCNGGA 2 cut(s) 51, 818
PinAI ACCGGT 1 cut(s) 238
PkrI GCNGC 3 cut(s) 61, 247, 628
PleI GAGTC 2 cut(s) 776, 1027
PluTI GGCGCC 1 cut(s) 609
PmaCI CACGTG 1 cut(s) 916
PmlI CACGTG 1 cut(s) 916
PpsI GAGTC 2 cut(s) 776, 1027
Ppu21I YACGTR 1 cut(s) 916
Psp124BI GAGCTC 1 cut(s) 509
Psp6I CCWGG 3 cut(s) 51, 443, 818
PspCI CACGTG 1 cut(s) 916
PspGI CCWGG 3 cut(s) 51, 443, 818
PspLI CGTACG 1 cut(s) 957
PspPI GGNCC 3 cut(s) 210, 236, 589
PsuI RGATCY 1 cut(s) 698
PsyI GACNNNGTC 1 cut(s) 671
RsaI GTAC 5 cut(s) 478, 815, 830, 886, 959
RsaNI GTAC 5 cut(s) 477, 814, 829, 885, 958
Rsr2I CGGWCCG 2 cut(s) 210, 236
RsrII CGGWCCG 2 cut(s) 210, 236
SacI GAGCTC 1 cut(s) 509
SacII CCGCGG 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 71
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 3 cut(s) 60, 246, 627
Sau3AI GATC 6 cut(s) 692, 698, 789, 843, 934, 948
Sau96I GGNCC 3 cut(s) 210, 236, 589
ScaI AGTACT 1 cut(s) 815
SchI GAGTC 2 cut(s) 777, 1028
ScrFI CCNGG 8 cut(s) 53, 223, 371, 445, 593, 709, 773, 820
SduI GDGCHC 4 cut(s) 90, 509, 657, 928
SfaNI GCATC 3 cut(s) 71, 933, 979
SfoI GGCGCC 1 cut(s) 607
Sfr303I CCGCGG 1 cut(s) 249
SgrBI CCGCGG 1 cut(s) 249
SinI GGWCC 2 cut(s) 210, 236
SmlI CTYRAG 1 cut(s) 340
SmoI CTYRAG 1 cut(s) 340
Sse9I AATT 1 cut(s) 412
SseBI AGGCCT 1 cut(s) 448
SspDI GGCGCC 1 cut(s) 605
SstI GAGCTC 1 cut(s) 509
StuI AGGCCT 1 cut(s) 448
StyD4I CCNGG 8 cut(s) 51, 221, 369, 443, 591, 707, 771, 818
StyI CCWWGG 1 cut(s) 863
TaaI ACNGT 3 cut(s) 120, 172, 889
TaiI ACGT 2 cut(s) 918, 959
TaqI TCGA 4 cut(s) 486, 503, 973, 1017
TaqII GACCGA 1 cut(s) 554
TasI AATT 1 cut(s) 412
TatI WGTACW 2 cut(s) 813, 884
TauI GCSGC 3 cut(s) 62, 248, 629
TfiI GAWTC 1 cut(s) 94
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 2 cut(s) 731, 894
TspDTI ATGAA 1 cut(s) 562
TspRI CASTG 2 cut(s) 731, 894
Tth111I GACNNNGTC 1 cut(s) 671
VpaK11BI GGWCC 2 cut(s) 210, 236
ZrmI AGTACT 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.