Rroxscaffold_3G00271230

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004478
Physical Location & Seq
Reverse (-)
63618615 .. 63622959
4345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00271230.1

Sequence Viewer

Length: 1275 bp
ATGGAACTCTGTGTCCCTTTGATTCTCTTTCTGATTTCTCTGCCAGCATTTTTGGCCAAAGATGTTGGACGTGCTAGCGTTGTAGTTGATGGGACAGTAGCTACTGCACACGTTGATAACAATTTTGTCTGTGCCACTCCTGATTGGTGGAATCATGAAGAGTGCAACTACAACCACTGTCCTTGGGGATCTGCATCTGTGTTAAATTTGAACTTGTCTCATCCTCTTCTTGCCAAGTCAATTCAAGGAGATTTGAAATATCCTTGCCATCCTTTCAGAAAGCAGAAGGGTGCGTTGTTTGGATTTTCTACAGGATGTTTACCTATGAGTAGATGGGATGAGCTTAACCATTTTTTCAGTAAGACGAGGCCCCTTGTCACATTTAGCTTGATGACAGGGAGGCACCACAAAGGCGCGGGTTTTTGGGTTGGAGATTGGGACTCTAGCAATGCTTATGATTTTATTAATTATACAGTTTCGAAGGGCTACCAGATAAATTCATGGGAGTTGGGTAATGAGCTCTGTGGTAATGGTGTTGGAGCTGAACAGTATGGAAAAGACGTGATCAAGCTTAAACAAATTGTCAACAAGTTGTACAATGACTCCCACATAAAGCCTGCAATTGTAGGACCTGGAGGATTCTATGATCAAAAGTGGTTTGCCACGCTTCTTCTGGTTTCTGGTTCAGGCGTAGTCAATGTTGTCACTCAGCATCTTTACAATCTGGGTCCAGGTGTTGATCCCAAGTTGATGCAAAAGATATTGGATCCATTTTACTTGGACCATATATCTGGTACATTCGATAATCTTGCAGCAACAGTGAAACAGCACGGACCTTGGGCTTCGATTTGGGTTGGAGAGTCTGGAGGAGCCTTTAACAGCGGTGGTCATAATGTATCTGACACATTTGTGAACAGCTTTTGGCAGTCCCTAGTTGGTGGGCACTACGGTCTTCTCAACAGAACCTCATTTCTTCCCAACCCTGATTACTACAGTGCACTTCTGTGGCATCAATTATGGGACAAAGAGTTCTTGCTGTTGACCGCAACGGGGCTCCACACTTGCGGGTATAACCCTACTCGTCATAAATTTAAGCATCAGACCGATTTCACTGTCAATGTTAAAAATGTTCTGGAGTTCAATACAGCTGCTCGATCAAGAAACACTGGCAAAGGAAGTAATTTCAGTCACGGACTTAAGAGAACGGTGTCATGGGTTGGTCTCAAAGCATCAGATGGACCGTTACACAGAAAAGAATACCATCTGATTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko05205 map00531 map01100 map05205
Pfam Domains
Protein Families
Glyco_hydro_79n

Protein Analysis

424

Amino Acids

47.23

Weight (kDa)

8.49

Isoelectric Point (pI)

25.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_79n PF03662 25 - 82 1.2e-19 Glycosyl hydrolase family 79, N-terminal domain
Glyco_hydro_79n PF03662 83 - 308 1.5e-97 Glycosyl hydrolase family 79, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 402
AccII CGCG 1 cut(s) 416
AciI CCGC 4 cut(s) 416, 882, 1044, 1065
AclWI GGATC 4 cut(s) 196, 734, 761, 774
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 3 cut(s) 205, 496, 1088
AfaI GTAC 2 cut(s) 596, 796
AfiI CCNNNNNNNGG 1 cut(s) 1050
AflII CTTAAG 1 cut(s) 1196
AflIII ACRYGT 1 cut(s) 109
AgsI TTSAA 4 cut(s) 211, 245, 256, 1141
AjiI CACGTC 2 cut(s) 71, 562
AjnI CCWGG 2 cut(s) 631, 730
AleI CACNNNNGTG 2 cut(s) 908, 1003
AloI GAACNNNNNNTCC 3 cut(s) 29, 1013, 1045
AluBI AGCT 8 cut(s) 101, 343, 387, 520, 542, 571, 918, 1148
AluI AGCT 8 cut(s) 101, 343, 387, 520, 542, 571, 918, 1148
Alw21I GWGCWC 2 cut(s) 522, 1000
Alw26I GTCTC 2 cut(s) 222, 1226
Alw44I GTGCAC 1 cut(s) 996
AlwI GGATC 4 cut(s) 196, 734, 761, 774
AoxI GGCC 2 cut(s) 54, 368
ApaLI GTGCAC 1 cut(s) 996
ApeKI GCWGC 2 cut(s) 812, 1148
ApoI RAATTY 3 cut(s) 205, 496, 1088
AseI ATTAAT 1 cut(s) 465
AspLEI GCGC 1 cut(s) 416
AspS9I GGNCC 6 cut(s) 369, 629, 728, 781, 833, 1238
AsuII TTCGAA 1 cut(s) 479
AsuNHI GCTAGC 1 cut(s) 74
AvaII GGWCC 5 cut(s) 629, 728, 781, 833, 1238
BaeGI GKGCMC 2 cut(s) 945, 1000
BalI TGGCCA 1 cut(s) 56
BamHI GGATCC 1 cut(s) 766
BanI GGYRCC 1 cut(s) 402
BanII GRGCYC 2 cut(s) 522, 1056
BbsI GAAGAC 1 cut(s) 944
Bbv12I GWGCWC 2 cut(s) 522, 1000
BbvI GCAGC 2 cut(s) 824, 1135
BccI CCATC 5 cut(s) 83, 276, 327, 1229, 1269
BcgI CGANNNNNNTGC 2 cut(s) 791, 825
BciT130I CCWGG 2 cut(s) 633, 732
BclI TGATCA 2 cut(s) 564, 646
BcoDI GTCTC 2 cut(s) 222, 1226
BfaI CTAG 3 cut(s) 75, 444, 932
BfmI CTRYAG 2 cut(s) 309, 991
BfrI CTTAAG 1 cut(s) 1196
BisI GCNGC 2 cut(s) 813, 1149
BlsI GCNGC 2 cut(s) 814, 1150
Bme1390I CCNGG 2 cut(s) 633, 732
Bme18I GGWCC 5 cut(s) 629, 728, 781, 833, 1238
BmgBI CACGTC 2 cut(s) 71, 562
BmgT120I GGNCC 6 cut(s) 369, 629, 728, 781, 833, 1238
BmiI GGNNCC 6 cut(s) 371, 404, 729, 768, 871, 1055
BmrFI CCNGG 2 cut(s) 633, 732
BmsI GCATC 6 cut(s) 203, 721, 741, 1018, 1105, 1238
BmtI GCTAGC 1 cut(s) 78
BpiI GAAGAC 1 cut(s) 944
BpmI CTGGAG 3 cut(s) 654, 885, 1154
Bpu14I TTCGAA 1 cut(s) 479
BsaBI GATNNNNATC 1 cut(s) 193
BsaI GGTCTC 1 cut(s) 1226
BsaJI CCNNGG 2 cut(s) 182, 836
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bsc4I CCNNNNNNNGG 1 cut(s) 1050
Bse1I ACTGG 1 cut(s) 1171
Bse3DI GCAATG 1 cut(s) 454
Bse8I GATNNNNATC 1 cut(s) 193
BseBI CCWGG 2 cut(s) 633, 732
BseDI CCNNGG 2 cut(s) 182, 836
BseGI GGATG 4 cut(s) 220, 268, 320, 343
BseJI GATNNNNATC 1 cut(s) 193
BseLI CCNNNNNNNGG 1 cut(s) 1050
BseMI GCAATG 1 cut(s) 454
BseMII CTCAG 1 cut(s) 722
BseNI ACTGG 1 cut(s) 1171
BseRI GAGGAG 1 cut(s) 882
BseSI GKGCMC 2 cut(s) 945, 1000
BseXI GCAGC 2 cut(s) 824, 1135
BsgI GTGCAG 1 cut(s) 90
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 2 cut(s) 56, 370
BshNI GGYRCC 1 cut(s) 402
BsiHKAI GWGCWC 2 cut(s) 522, 1000
BslFI GGGAC 4 cut(s) 106, 452, 913, 1034
BslI CCNNNNNNNGG 1 cut(s) 1050
BsmAI GTCTC 2 cut(s) 222, 1226
BsmFI GGGAC 4 cut(s) 106, 452, 913, 1034
BsnI GGCC 2 cut(s) 56, 370
Bso31I GGTCTC 1 cut(s) 1226
Bsp119I TTCGAA 1 cut(s) 479
Bsp1286I GDGCHC 4 cut(s) 522, 945, 1000, 1056
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 6 cut(s) 188, 564, 646, 739, 766, 1154
BspACI CCGC 4 cut(s) 416, 882, 1044, 1065
BspANI GGCC 2 cut(s) 56, 370
BspCNI CTCAG 1 cut(s) 721
BspFNI CGCG 1 cut(s) 416
BspHI TCATGA 1 cut(s) 154
BspLI GGNNCC 6 cut(s) 371, 404, 729, 768, 871, 1055
BspOI GCTAGC 1 cut(s) 78
BspPI GGATC 4 cut(s) 196, 734, 761, 774
BspT104I TTCGAA 1 cut(s) 479
BspT107I GGYRCC 1 cut(s) 402
BspTI CTTAAG 1 cut(s) 1196
BspTNI GGTCTC 1 cut(s) 1226
BsrDI GCAATG 1 cut(s) 454
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 1 cut(s) 1171
BssECI CCNNGG 2 cut(s) 182, 836
BssMI GATC 6 cut(s) 188, 564, 646, 739, 766, 1154
BssT1I CCWWGG 2 cut(s) 182, 836
Bst2UI CCWGG 2 cut(s) 633, 732
Bst6I CTCTTC 2 cut(s) 153, 231
BstAFI CTTAAG 1 cut(s) 1196
BstAUI TGTACA 1 cut(s) 594
BstBI TTCGAA 1 cut(s) 479
BstC8I GCNNGC 3 cut(s) 45, 76, 618
BstDEI CTNAG 1 cut(s) 708
BstF5I GGATG 4 cut(s) 220, 268, 320, 343
BstFNI CGCG 1 cut(s) 416
BstHHI GCGC 1 cut(s) 416
BstKTI GATC 6 cut(s) 191, 567, 649, 742, 769, 1157
BstMAI GTCTC 2 cut(s) 222, 1226
BstMBI GATC 6 cut(s) 188, 564, 646, 739, 766, 1154
BstMWI GCNNNNNNNGC 1 cut(s) 53
BstNI CCWGG 2 cut(s) 633, 732
BstSCI CCNGG 2 cut(s) 631, 730
BstSFI CTRYAG 2 cut(s) 309, 991
BstSLI GKGCMC 2 cut(s) 945, 1000
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 2 cut(s) 824, 1135
BstV2I GAAGAC 1 cut(s) 944
BstX2I RGATCY 2 cut(s) 188, 766
BstXI CCANNNNNNTGG 1 cut(s) 791
BstYI RGATCY 2 cut(s) 188, 766
BsuRI GGCC 2 cut(s) 56, 370
BtrI CACGTC 2 cut(s) 71, 562
BtsCI GGATG 4 cut(s) 220, 268, 320, 343
BtsIMutI CAGTG 5 cut(s) 175, 825, 1000, 1110, 1164
Cac8I GCNNGC 3 cut(s) 45, 76, 618
CciI TCATGA 1 cut(s) 154
CfoI GCGC 1 cut(s) 416
Cfr13I GGNCC 6 cut(s) 369, 629, 728, 781, 833, 1238
Csp6I GTAC 2 cut(s) 595, 795
CspCI CAANNNNNGTGG 2 cut(s) 164, 199
CviAII CATG 3 cut(s) 155, 501, 1212
CviQI GTAC 2 cut(s) 595, 795
DdeI CTNAG 1 cut(s) 708
DpnI GATC 6 cut(s) 190, 566, 648, 741, 768, 1156
DpnII GATC 6 cut(s) 188, 564, 646, 739, 766, 1154
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 2 cut(s) 153, 231
EarI CTCTTC 2 cut(s) 153, 231
Ecl136II GAGCTC 1 cut(s) 520
Eco130I CCWWGG 2 cut(s) 182, 836
Eco24I GRGCYC 2 cut(s) 522, 1056
Eco31I GGTCTC 1 cut(s) 1226
Eco47I GGWCC 5 cut(s) 629, 728, 781, 833, 1238
Eco53kI GAGCTC 1 cut(s) 520
EcoICRI GAGCTC 1 cut(s) 520
EcoO109I RGGNCCY 2 cut(s) 369, 629
EcoRII CCWGG 2 cut(s) 631, 730
EcoT14I CCWWGG 2 cut(s) 182, 836
EcoT38I GRGCYC 2 cut(s) 522, 1056
ErhI CCWWGG 2 cut(s) 182, 836
FaeI CATG 3 cut(s) 158, 504, 1215
FaqI GGGAC 4 cut(s) 106, 452, 913, 1034
FatI CATG 3 cut(s) 154, 500, 1211
FauI CCCGC 2 cut(s) 409, 1058
FbaI TGATCA 2 cut(s) 564, 646
Fnu4HI GCNGC 2 cut(s) 813, 1149
FokI GGATG 4 cut(s) 207, 255, 327, 350
FriOI GRGCYC 2 cut(s) 522, 1056
Fsp4HI GCNGC 2 cut(s) 813, 1149
FspBI CTAG 3 cut(s) 75, 444, 932
GlaI GCGC 1 cut(s) 415
GluI GCNGC 2 cut(s) 813, 1149
GsuI CTGGAG 3 cut(s) 654, 885, 1154
HaeIII GGCC 2 cut(s) 56, 370
HhaI GCGC 1 cut(s) 416
Hin1II CATG 3 cut(s) 158, 504, 1215
Hin6I GCGC 1 cut(s) 414
HinP1I GCGC 1 cut(s) 414
HincII GTYRAC 2 cut(s) 586, 1041
HindII GTYRAC 2 cut(s) 586, 1041
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 7 cut(s) 22, 151, 440, 602, 639, 860, 1267
Hpy166II GTNNAC 5 cut(s) 320, 586, 913, 998, 1041
Hpy188I TCNGA 6 cut(s) 33, 278, 901, 1101, 1234, 1266
Hpy188III TCNNGA 5 cut(s) 140, 155, 864, 1133, 1158
Hpy8I GTNNAC 5 cut(s) 320, 586, 913, 998, 1041
HpyAV CCTTC 2 cut(s) 280, 475
HpyCH4IV ACGT 3 cut(s) 70, 111, 561
HpyCH4V TGCA 7 cut(s) 107, 165, 194, 620, 754, 812, 998
HpyF10VI GCNNNNNNNGC 1 cut(s) 53
HpyF3I CTNAG 1 cut(s) 708
HpySE526I ACGT 3 cut(s) 70, 111, 561
Hsp92II CATG 3 cut(s) 158, 504, 1215
HspAI GCGC 1 cut(s) 414
Ksp22I TGATCA 2 cut(s) 564, 646
Kzo9I GATC 6 cut(s) 188, 564, 646, 739, 766, 1154
LmnI GCTCC 3 cut(s) 539, 869, 1059
Lsp1109I GCAGC 2 cut(s) 824, 1135
LweI GCATC 6 cut(s) 203, 721, 741, 1018, 1105, 1238
MaeI CTAG 3 cut(s) 75, 444, 932
MaeII ACGT 3 cut(s) 70, 111, 561
MaeIII GTNAC 4 cut(s) 376, 703, 1187, 1242
MalI GATC 6 cut(s) 190, 566, 648, 741, 768, 1156
MboI GATC 6 cut(s) 188, 564, 646, 739, 766, 1154
MboII GAAGA 5 cut(s) 170, 218, 662, 944, 965
MfeI CAATTG 1 cut(s) 621
MflI RGATCY 2 cut(s) 188, 766
MhlI GDGCHC 4 cut(s) 522, 945, 1000, 1056
MlsI TGGCCA 1 cut(s) 56
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 3 cut(s) 434, 596, 869
MmeI TCCRAC 4 cut(s) 46, 409, 517, 835
MnlI CCTC 6 cut(s) 234, 360, 393, 629, 860, 976
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MseI TTAA 8 cut(s) 203, 345, 465, 573, 876, 1092, 1122, 1197
MslI CAYNNNNRTG 2 cut(s) 908, 1003
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 2 cut(s) 882, 1148
MspCI CTTAAG 1 cut(s) 1196
MspR9I CCNGG 2 cut(s) 633, 732
MunI CAATTG 1 cut(s) 621
MvaI CCWGG 2 cut(s) 633, 732
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 1 cut(s) 53
NdeII GATC 6 cut(s) 188, 564, 646, 739, 766, 1154
NheI GCTAGC 1 cut(s) 74
NlaIII CATG 3 cut(s) 158, 504, 1215
NlaIV GGNNCC 6 cut(s) 371, 404, 729, 768, 871, 1055
NmuCI GTSAC 3 cut(s) 376, 703, 1187
NspV TTCGAA 1 cut(s) 479
OliI CACNNNNGTG 2 cut(s) 908, 1003
PagI TCATGA 1 cut(s) 154
PfeI GAWTC 4 cut(s) 22, 151, 639, 1267
PkrI GCNGC 2 cut(s) 814, 1150
PleI GAGTC 3 cut(s) 434, 596, 868
PpsI GAGTC 3 cut(s) 434, 596, 868
PpuMI RGGWCCY 1 cut(s) 629
PshBI ATTAAT 1 cut(s) 465
Psp124BI GAGCTC 1 cut(s) 522
Psp5II RGGWCCY 1 cut(s) 629
Psp6I CCWGG 2 cut(s) 631, 730
PspGI CCWGG 2 cut(s) 631, 730
PspN4I GGNNCC 6 cut(s) 371, 404, 729, 768, 871, 1055
PspPI GGNCC 6 cut(s) 369, 629, 728, 781, 833, 1238
PspPPI RGGWCCY 1 cut(s) 629
PsuI RGATCY 2 cut(s) 188, 766
PvuII CAGCTG 1 cut(s) 1148
RsaI GTAC 2 cut(s) 596, 796
RsaNI GTAC 2 cut(s) 595, 795
RseI CAYNNNNRTG 2 cut(s) 908, 1003
SacI GAGCTC 1 cut(s) 522
SaqAI TTAA 8 cut(s) 203, 345, 465, 573, 876, 1092, 1122, 1197
SatI GCNGC 2 cut(s) 813, 1149
Sau3AI GATC 6 cut(s) 188, 564, 646, 739, 766, 1154
Sau96I GGNCC 6 cut(s) 369, 629, 728, 781, 833, 1238
SchI GAGTC 3 cut(s) 434, 596, 869
ScrFI CCNGG 2 cut(s) 633, 732
SduI GDGCHC 4 cut(s) 522, 945, 1000, 1056
SfaNI GCATC 6 cut(s) 203, 721, 741, 1018, 1105, 1238
SfcI CTRYAG 2 cut(s) 309, 991
SfuI TTCGAA 1 cut(s) 479
SinI GGWCC 5 cut(s) 629, 728, 781, 833, 1238
SmiMI CAYNNNNRTG 2 cut(s) 908, 1003
SmlI CTYRAG 1 cut(s) 1196
SmoI CTYRAG 1 cut(s) 1196
SsiI CCGC 4 cut(s) 416, 882, 1044, 1065
SspMI CTAG 3 cut(s) 75, 444, 932
SstI GAGCTC 1 cut(s) 522
StyD4I CCNGG 2 cut(s) 631, 730
StyI CCWWGG 2 cut(s) 182, 836
TaiI ACGT 3 cut(s) 73, 114, 564
TaqI TCGA 4 cut(s) 479, 801, 845, 1153
TaqII GACCGA 1 cut(s) 1118
TatI WGTACW 1 cut(s) 594
TfiI GAWTC 4 cut(s) 22, 151, 639, 1267
Tru1I TTAA 8 cut(s) 203, 345, 465, 573, 876, 1092, 1122, 1197
Tru9I TTAA 8 cut(s) 203, 345, 465, 573, 876, 1092, 1122, 1197
TscAI CASTG 5 cut(s) 182, 825, 1000, 1117, 1171
TseFI GTSAC 3 cut(s) 376, 703, 1187
TseI GCWGC 2 cut(s) 812, 1148
Tsp45I GTSAC 3 cut(s) 376, 703, 1187
TspDTI ATGAA 2 cut(s) 171, 489
TspGWI ACGGA 2 cut(s) 846, 1206
TspRI CASTG 5 cut(s) 182, 825, 1000, 1117, 1171
Vha464I CTTAAG 1 cut(s) 1196
VneI GTGCAC 1 cut(s) 996
VpaK11BI GGWCC 5 cut(s) 629, 728, 781, 833, 1238
VspI ATTAAT 1 cut(s) 465
XapI RAATTY 3 cut(s) 205, 496, 1088
XcmI CCANNNNNNNNNTGG 1 cut(s) 670
XspI CTAG 3 cut(s) 75, 444, 932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.