pycom14g17220

heparanase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004478
Physical Location & Seq
Forward (+)
19259226 .. 19260680
1455 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g17220.4

Sequence Viewer

Length: 912 bp
ATGGAATTCCGTCTCTCCTTGGTCCTCTTTCTGGTTTCTCTCCCTGCAATTTTAGCTCAAGAATTTGGAAAAGCTAATAATGTTGAAAATGTTAAGGTTGTGGTTAACGGCATTTTATCTGTTGCACGAGTTGACAATAACTTTGTCTGTGCCACTATTGATTGGTGGAATAATGAAAAGTGCAACTACGGCCACTGTCCATGGGGACATTCATCCGCGTTAAATTTGAACTTGTCTCATCCTTTGCTAGCCAAGTCAATTCAAGCTTTCGATCAGTTGAGAATCAGAATTGGAGGTTCCTTGGAAGACCAACTATTGTACGATGTTGGAAATTTGAAATATCCTTGCCATCCTTTCAGAAAGCTGGAGAATGGCTTGTTTGGATTCTCTAGAGGATGTTTAAATATGAGTAGATGGGATGAGTTGAACCAGTTTTTCAGCAAAACAAGGTCCATCGTCACGTTTAGCTTGAATGCACTGTTGGGGAGGCACCACAAAGGTGGGGGAGTTTGGGTAGGAGAATGGGACTCTAGCAACGCTTATGATTTTATTAATTATACCGTCTCAAAGAGATACGAGATAGATTCATGGGAATTTGGAAATGAGCTCAGTGGAAGTGGTATTGGAGCTAGTGTTGGAGCTGGACAATATGGAAGAGATTTGATCAAGCTTAAACACATCATCAACCATTTGTACAATGATTCCGTTGCTAAACCTGCACTTATGGCACCTGGAGGATTCTTTGACCAATCATGGTTTGCCAGGCTTCTTCAGGTTACTGGTTCTGGCACAGTCGATATCATCTCTCAGCATATCTACAATCTGGGTGCAGGTGTTGATCCCAAGTTGGTGAAAAAAATATTGAATCCCCATTACTTGAATCGTGCATACGGGCTGTTCGATCGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko05205 map00531 map01100 map05205
Pfam Domains
Protein Families
Glyco_hydro_79n

Protein Analysis

304

Amino Acids

33.9

Weight (kDa)

8.51

Isoelectric Point (pI)

30.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 821
Acc36I ACCTGC 2 cut(s) 724, 821
AccB1I GGYRCC 2 cut(s) 489, 727
AccII CGCG 1 cut(s) 218
AciI CCGC 1 cut(s) 216
AclWI GGATC 1 cut(s) 833
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 5 cut(s) 5, 62, 223, 331, 593
AcuI CTGAAG 1 cut(s) 755
AfaI GTAC 2 cut(s) 320, 695
AfiI CCNNNNNNNGG 1 cut(s) 31
AgsI TTSAA 8 cut(s) 86, 229, 263, 337, 427, 472, 865, 880
AjnI CCWGG 2 cut(s) 730, 761
AjuI GAANNNNNNNTTGG 2 cut(s) 464, 496
AleI CACNNNNGTG 1 cut(s) 498
AluBI AGCT 9 cut(s) 56, 74, 266, 364, 468, 607, 629, 641, 670
AluI AGCT 9 cut(s) 56, 74, 266, 364, 468, 607, 629, 641, 670
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 3 cut(s) 17, 240, 568
AlwI GGATC 1 cut(s) 833
AoxI GGCC 1 cut(s) 190
ApoI RAATTY 5 cut(s) 5, 62, 223, 331, 593
ArsI GACNNNNNNTTYG 2 cut(s) 125, 157
AseI ATTAAT 1 cut(s) 552
AspS9I GGNCC 2 cut(s) 22, 450
AsuHPI GGTGA 1 cut(s) 862
AsuNHI GCTAGC 1 cut(s) 247
AvaII GGWCC 2 cut(s) 22, 450
BanI GGYRCC 2 cut(s) 489, 727
BanII GRGCYC 1 cut(s) 609
BauI CACGAG 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 609
BccI CCATC 3 cut(s) 357, 408, 461
BceAI ACGGC 2 cut(s) 124, 205
BciT130I CCWGG 2 cut(s) 732, 763
BclI TGATCA 1 cut(s) 663
BcoDI GTCTC 3 cut(s) 17, 240, 568
BfaI CTAG 4 cut(s) 248, 390, 531, 630
BfuAI ACCTGC 2 cut(s) 724, 821
Bme1390I CCNGG 2 cut(s) 732, 763
Bme18I GGWCC 2 cut(s) 22, 450
BmgT120I GGNCC 2 cut(s) 22, 450
BmiI GGNNCC 3 cut(s) 298, 491, 729
BmrFI CCNGG 2 cut(s) 732, 763
BmtI GCTAGC 1 cut(s) 251
BpiI GAAGAC 1 cut(s) 312
BpmI CTGGAG 2 cut(s) 386, 753
BpuEI CTTGAG 1 cut(s) 42
Bsa29I ATCGAT 1 cut(s) 904
BsaJI CCNNGG 3 cut(s) 18, 200, 300
BsaXI ACNNNNNCTCC 4 cut(s) 498, 528, 618, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse1I ACTGG 2 cut(s) 430, 784
BseBI CCWGG 2 cut(s) 732, 763
BseCI ATCGAT 1 cut(s) 904
BseDI CCNNGG 3 cut(s) 18, 200, 300
BseGI GGATG 5 cut(s) 212, 238, 349, 401, 424
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMII CTCAG 2 cut(s) 622, 821
BseNI ACTGG 2 cut(s) 430, 784
BsgI GTGCAG 2 cut(s) 702, 849
Bsh1236I CGCG 1 cut(s) 218
Bsh1285I CGRYCG 1 cut(s) 904
BshFI GGCC 1 cut(s) 192
BshNI GGYRCC 2 cut(s) 489, 727
BshVI ATCGAT 1 cut(s) 904
BsiEI CGRYCG 1 cut(s) 904
BsiHKAI GWGCWC 1 cut(s) 609
BslFI GGGAC 2 cut(s) 219, 539
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 3 cut(s) 17, 240, 568
BsmBI CGTCTC 2 cut(s) 17, 568
BsmFI GGGAC 2 cut(s) 219, 539
BsmI GAATGC 1 cut(s) 478
BsnI GGCC 1 cut(s) 192
Bsp1286I GDGCHC 1 cut(s) 609
Bsp1407I TGTACA 1 cut(s) 693
Bsp143I GATC 5 cut(s) 271, 663, 838, 901, 905
Bsp19I CCATGG 1 cut(s) 200
BspACI CCGC 1 cut(s) 216
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 2 cut(s) 621, 820
BspDI ATCGAT 1 cut(s) 904
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 3 cut(s) 298, 491, 729
BspMI ACCTGC 2 cut(s) 724, 821
BspOI GCTAGC 1 cut(s) 251
BspPI GGATC 1 cut(s) 833
BspT107I GGYRCC 2 cut(s) 489, 727
BsrGI TGTACA 1 cut(s) 693
BsrI ACTGG 2 cut(s) 430, 784
BssECI CCNNGG 3 cut(s) 18, 200, 300
BssMI GATC 5 cut(s) 271, 663, 838, 901, 905
BssSI CACGAG 1 cut(s) 126
BssT1I CCWWGG 3 cut(s) 18, 200, 300
Bst2BI CACGAG 1 cut(s) 126
Bst2UI CCWGG 2 cut(s) 732, 763
Bst4CI ACNGT 4 cut(s) 197, 480, 562, 793
Bst6I CTCTTC 1 cut(s) 649
BstAUI TGTACA 1 cut(s) 693
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 2 cut(s) 608, 807
BstDSI CCRYGG 1 cut(s) 200
BstF5I GGATG 5 cut(s) 212, 238, 349, 401, 424
BstFNI CGCG 1 cut(s) 218
BstKTI GATC 5 cut(s) 274, 666, 841, 904, 908
BstMAI GTCTC 3 cut(s) 17, 240, 568
BstMBI GATC 5 cut(s) 271, 663, 838, 901, 905
BstMCI CGRYCG 1 cut(s) 904
BstMWI GCNNNNNNNGC 4 cut(s) 53, 189, 716, 725
BstNI CCWGG 2 cut(s) 732, 763
BstSCI CCNGG 2 cut(s) 730, 761
BstUI CGCG 1 cut(s) 218
BstV2I GAAGAC 1 cut(s) 312
BstXI CCANNNNNNTGG 1 cut(s) 500
Bsu15I ATCGAT 1 cut(s) 904
BsuRI GGCC 1 cut(s) 192
BsuTUI ATCGAT 1 cut(s) 904
BtgI CCRYGG 1 cut(s) 200
BtsCI GGATG 5 cut(s) 212, 238, 349, 401, 424
BtsIMutI CAGTG 3 cut(s) 193, 476, 616
BveI ACCTGC 2 cut(s) 724, 821
Cac8I GCNNGC 1 cut(s) 249
Cfr13I GGNCC 2 cut(s) 22, 450
ClaI ATCGAT 1 cut(s) 904
Csp6I GTAC 2 cut(s) 319, 694
CviAII CATG 3 cut(s) 201, 588, 753
CviQI GTAC 2 cut(s) 319, 694
DdeI CTNAG 2 cut(s) 608, 807
DpnI GATC 5 cut(s) 273, 665, 840, 903, 907
DpnII GATC 5 cut(s) 271, 663, 838, 901, 905
DraI TTTAAA 1 cut(s) 402
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 649
EarI CTCTTC 1 cut(s) 649
Ecl136II GAGCTC 1 cut(s) 607
Eco130I CCWWGG 3 cut(s) 18, 200, 300
Eco24I GRGCYC 1 cut(s) 609
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 2 cut(s) 22, 450
Eco53kI GAGCTC 1 cut(s) 607
Eco57I CTGAAG 1 cut(s) 755
EcoICRI GAGCTC 1 cut(s) 607
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 2 cut(s) 730, 761
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 3 cut(s) 18, 200, 300
EcoT38I GRGCYC 1 cut(s) 609
ErhI CCWWGG 3 cut(s) 18, 200, 300
Esp3I CGTCTC 2 cut(s) 17, 568
FaeI CATG 3 cut(s) 204, 591, 756
FaqI GGGAC 2 cut(s) 219, 539
FatI CATG 3 cut(s) 200, 587, 752
FbaI TGATCA 1 cut(s) 663
FokI GGATG 5 cut(s) 199, 225, 336, 408, 431
FriOI GRGCYC 1 cut(s) 609
FspBI CTAG 4 cut(s) 248, 390, 531, 630
GsuI CTGGAG 2 cut(s) 386, 753
HaeIII GGCC 1 cut(s) 192
Hin1II CATG 3 cut(s) 204, 591, 756
HincII GTYRAC 2 cut(s) 106, 133
HindII GTYRAC 2 cut(s) 106, 133
HindIII AAGCTT 2 cut(s) 264, 668
HinfI GANTC 8 cut(s) 282, 384, 527, 584, 701, 738, 865, 880
HpaI GTTAAC 1 cut(s) 106
HphI GGTGA 1 cut(s) 862
Hpy166II GTNNAC 2 cut(s) 106, 133
Hpy188I TCNGA 2 cut(s) 287, 359
Hpy188III TCNNGA 3 cut(s) 59, 390, 909
Hpy8I GTNNAC 2 cut(s) 106, 133
HpyCH4III ACNGT 4 cut(s) 197, 480, 562, 793
HpyCH4IV ACGT 1 cut(s) 461
HpyCH4V TGCA 7 cut(s) 47, 125, 183, 476, 719, 830, 887
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 189, 716, 725
HpyF3I CTNAG 2 cut(s) 608, 807
HpySE526I ACGT 1 cut(s) 461
Hsp92II CATG 3 cut(s) 204, 591, 756
Ksp22I TGATCA 1 cut(s) 663
KspAI GTTAAC 1 cut(s) 106
Kzo9I GATC 5 cut(s) 271, 663, 838, 901, 905
LmnI GCTCC 2 cut(s) 626, 638
MaeI CTAG 4 cut(s) 248, 390, 531, 630
MaeII ACGT 1 cut(s) 461
MaeIII GTNAC 2 cut(s) 457, 775
MalI GATC 5 cut(s) 273, 665, 840, 903, 907
MboI GATC 5 cut(s) 271, 663, 838, 901, 905
MboII GAAGA 3 cut(s) 317, 666, 761
MhlI GDGCHC 1 cut(s) 609
MluCI AATT 9 cut(s) 5, 48, 62, 223, 258, 288, 331, 553, 593
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 2 cut(s) 307, 616
MnlI CCTC 5 cut(s) 35, 287, 386, 480, 728
MseI TTAA 6 cut(s) 93, 105, 221, 401, 552, 672
MslI CAYNNNNRTG 1 cut(s) 498
MspR9I CCNGG 2 cut(s) 732, 763
Mva1269I GAATGC 1 cut(s) 478
MvaI CCWGG 2 cut(s) 732, 763
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 4 cut(s) 53, 189, 716, 725
NcoI CCATGG 1 cut(s) 200
NdeII GATC 5 cut(s) 271, 663, 838, 901, 905
NheI GCTAGC 1 cut(s) 247
NlaIII CATG 3 cut(s) 204, 591, 756
NlaIV GGNNCC 3 cut(s) 298, 491, 729
NmuCI GTSAC 1 cut(s) 457
OliI CACNNNNGTG 1 cut(s) 498
PaqCI CACCTGC 1 cut(s) 821
PcsI WCGNNNNNNNCGW 1 cut(s) 897
PctI GAATGC 1 cut(s) 478
PfeI GAWTC 7 cut(s) 282, 384, 584, 701, 738, 865, 880
Ple19I CGATCG 1 cut(s) 904
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
PshBI ATTAAT 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 609
Psp6I CCWGG 2 cut(s) 730, 761
PspGI CCWGG 2 cut(s) 730, 761
PspN4I GGNNCC 3 cut(s) 298, 491, 729
PspPI GGNCC 2 cut(s) 22, 450
PvuI CGATCG 1 cut(s) 904
RsaI GTAC 2 cut(s) 320, 695
RsaNI GTAC 2 cut(s) 319, 694
RseI CAYNNNNRTG 1 cut(s) 498
SacI GAGCTC 1 cut(s) 609
SaqAI TTAA 6 cut(s) 93, 105, 221, 401, 552, 672
Sau3AI GATC 5 cut(s) 271, 663, 838, 901, 905
Sau96I GGNCC 2 cut(s) 22, 450
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 2 cut(s) 732, 763
SduI GDGCHC 1 cut(s) 609
SinI GGWCC 2 cut(s) 22, 450
SmiMI CAYNNNNRTG 1 cut(s) 498
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
Sse9I AATT 9 cut(s) 5, 48, 62, 223, 258, 288, 331, 553, 593
SsiI CCGC 1 cut(s) 216
SspI AATATT 1 cut(s) 861
SspMI CTAG 4 cut(s) 248, 390, 531, 630
SstI GAGCTC 1 cut(s) 609
StyD4I CCNGG 2 cut(s) 730, 761
StyI CCWWGG 3 cut(s) 18, 200, 300
TaaI ACNGT 4 cut(s) 197, 480, 562, 793
TaiI ACGT 1 cut(s) 464
TaqI TCGA 4 cut(s) 270, 795, 900, 904
TasI AATT 9 cut(s) 5, 48, 62, 223, 258, 288, 331, 553, 593
TatI WGTACW 1 cut(s) 693
TfiI GAWTC 7 cut(s) 282, 384, 584, 701, 738, 865, 880
Tru1I TTAA 6 cut(s) 93, 105, 221, 401, 552, 672
Tru9I TTAA 6 cut(s) 93, 105, 221, 401, 552, 672
TscAI CASTG 3 cut(s) 200, 483, 616
TseFI GTSAC 1 cut(s) 457
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 3 cut(s) 189, 201, 576
TspGWI ACGGA 1 cut(s) 694
TspRI CASTG 3 cut(s) 200, 483, 616
VpaK11BI GGWCC 2 cut(s) 22, 450
VspI ATTAAT 1 cut(s) 552
XapI RAATTY 5 cut(s) 5, 62, 223, 331, 593
XbaI TCTAGA 1 cut(s) 389
XspI CTAG 4 cut(s) 248, 390, 531, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.