pycom06g18190

COBRA-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013790
Physical Location & Seq
Forward (+)
22019409 .. 22020646
1238 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g18190.3

Sequence Viewer

Length: 735 bp
ATGATGACCTGGAACGTTACTTGCACGTACTCGCAATTCCTAGCTCAAAAGACACCCACGTGTTGTGTGTCGCTGTCTTCCTTCTATAATCAAACCATCGTGAGTTGTCCCACTTGTGCGTGTGGCTGTCAGAACAACGCTACAGATCCAGGAAGCTGCGTAGAGCCAAACAATCCATACTTGGCTTCAGCTGTATCTGGTTCCGGGAAATCTGCTAATACACCTCTTGTCCAGTGTACCAGTCATATGTGTCCCATCCGAGTCCATTGGCATGTTAAGCTCAATTACAAGGAGTACTGGAGGGTGAAAGTGACAATTACAAATTTCAATTACAGAATGAACTACACACTGTGGAATCTTGTCGTGCAACATCCGAACTTTGATAATTTGACCAAGATTTTCAGTTTCAATTACAAATCATTAACTCCTTATGTTGGCTTAAATGATACGGCCATGTTGTGGGGAGTAAAGTTTTATAACGATTTCCTCTCGCAAGCTGGTCCCCTTGGAAACGTGCAGTCAGAGCTCCTATTTCGAAAGGATACATCAACTTTTACTTTTGAAAAGGGTTGGGCTTTCCCTCGAAGGATTTATTTCAACGGAGACAACTGTGTCATGCCACCTCCAGATTCCTACCCATGGCTGCCTAATGCCAGTCCCAAACGAATCATTAGCGTACTTAGTCCAGTCATGACCGTCTTCACATCTCTGGTATTCTTATTGGCTTATGCATAG

Protein Analysis

245

Amino Acids

27.66

Weight (kDa)

8.84

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60920 AT5G60920
fragaria_vesca FvH4_5g13550
malus_domestica MD06G1203700.v1.1 MD14G1214800.v1.1
prunus_persica Prupe.5G207800_v2.0.a1
pyrus_communis pycom06g18190 pycom14g17790
rosa_chinensis RchiOBHm_Chr7g0183381
rosa_laevigata RLG00000005051
rosa_multiflora Rmu_sc0000951.1_g000012
rosa_roxburghii Rroxscaffold_3G00270490
rosa_rugosa Rorug06G0457600
rosa_samantha Rh7AG058700 Rh7BG058700 Rh7DG058400
rosa_wichuraiana Rw7G004830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 477
AasI GACNNNNNNGTC 1 cut(s) 611
AccB7I CCANNNNNTGG 1 cut(s) 459
AclI AACGTT 1 cut(s) 15
AclWI GGATC 1 cut(s) 140
AcoI YGGCCR 1 cut(s) 450
AcsI RAATTY 1 cut(s) 322
AcuI CTGAAG 1 cut(s) 171
AcvI CACGTG 1 cut(s) 60
AdeI CACNNNGTG 1 cut(s) 351
AfaI GTAC 4 cut(s) 29, 238, 296, 678
AfiI CCNNNNNNNGG 3 cut(s) 434, 459, 639
AflIII ACRYGT 1 cut(s) 59
AgsI TTSAA 4 cut(s) 328, 409, 563, 598
AjnI CCWGG 2 cut(s) 8, 148
AleI CACNNNNGTG 1 cut(s) 58
AluBI AGCT 6 cut(s) 44, 156, 191, 280, 497, 526
AluI AGCT 6 cut(s) 44, 156, 191, 280, 497, 526
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 1 cut(s) 597
AlwI GGATC 1 cut(s) 140
AoxI GGCC 1 cut(s) 450
ApeKI GCWGC 2 cut(s) 156, 643
ApoI RAATTY 1 cut(s) 322
AspS9I GGNCC 1 cut(s) 500
AsuC2I CCSGG 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 316
AsuII TTCGAA 1 cut(s) 535
AvaII GGWCC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 528
BbrPI CACGTG 1 cut(s) 60
BbsI GAAGAC 2 cut(s) 69, 691
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 2 cut(s) 143, 630
BccI CCATC 2 cut(s) 104, 263
BceAI ACGGC 1 cut(s) 465
BciT130I CCWGG 2 cut(s) 10, 150
BciVI GTATCC 1 cut(s) 535
BcnI CCSGG 1 cut(s) 205
BcoDI GTCTC 1 cut(s) 597
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 141
BfuI GTATCC 1 cut(s) 535
BisI GCNGC 2 cut(s) 157, 644
BlsI GCNGC 2 cut(s) 158, 645
BmcAI AGTACT 1 cut(s) 296
Bme1390I CCNGG 3 cut(s) 10, 150, 205
Bme18I GGWCC 1 cut(s) 500
BmgT120I GGNCC 1 cut(s) 500
BmiI GGNNCC 2 cut(s) 202, 502
BmrFI CCNGG 3 cut(s) 10, 150, 205
BpiI GAAGAC 2 cut(s) 69, 691
BpmI CTGGAG 2 cut(s) 319, 609
Bpu14I TTCGAA 1 cut(s) 535
BpuMI CCSGG 1 cut(s) 205
BsaAI YACGTR 2 cut(s) 27, 60
BsaJI CCNNGG 2 cut(s) 505, 638
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 459, 639
Bse1I ACTGG 5 cut(s) 232, 240, 302, 654, 686
BseBI CCWGG 2 cut(s) 10, 150
BseDI CCNNGG 2 cut(s) 505, 638
BseGI GGATG 2 cut(s) 255, 370
BseLI CCNNNNNNNGG 3 cut(s) 434, 459, 639
BseNI ACTGG 5 cut(s) 232, 240, 302, 654, 686
BseXI GCAGC 2 cut(s) 143, 630
BsgI GTGCAG 1 cut(s) 536
BshFI GGCC 1 cut(s) 452
BsiHKAI GWGCWC 1 cut(s) 528
BsiSI CCGG 1 cut(s) 204
BslFI GGGAC 4 cut(s) 93, 237, 486, 642
BslI CCNNNNNNNGG 3 cut(s) 434, 459, 639
BsmAI GTCTC 1 cut(s) 597
BsmFI GGGAC 4 cut(s) 93, 237, 486, 642
BsnI GGCC 1 cut(s) 452
Bsp119I TTCGAA 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 528
Bsp143I GATC 1 cut(s) 145
Bsp19I CCATGG 1 cut(s) 638
BspANI GGCC 1 cut(s) 452
BspHI TCATGA 1 cut(s) 690
BspLI GGNNCC 2 cut(s) 202, 502
BspPI GGATC 1 cut(s) 140
BspT104I TTCGAA 1 cut(s) 535
BsrI ACTGG 5 cut(s) 232, 240, 302, 654, 686
BssECI CCNNGG 2 cut(s) 505, 638
BssMI GATC 1 cut(s) 145
BssT1I CCWWGG 2 cut(s) 505, 638
Bst2UI CCWGG 2 cut(s) 10, 150
Bst4CI ACNGT 3 cut(s) 351, 611, 697
BstBAI YACGTR 2 cut(s) 27, 60
BstBI TTCGAA 1 cut(s) 535
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 1 cut(s) 680
BstDSI CCRYGG 1 cut(s) 638
BstF5I GGATG 2 cut(s) 255, 370
BstKTI GATC 1 cut(s) 148
BstMAI GTCTC 1 cut(s) 597
BstMBI GATC 1 cut(s) 145
BstMWI GCNNNNNNNGC 2 cut(s) 277, 523
BstNI CCWGG 2 cut(s) 10, 150
BstNSI RCATGY 1 cut(s) 275
BstSCI CCNGG 3 cut(s) 8, 148, 203
BstSFI CTRYAG 1 cut(s) 141
BstV1I GCAGC 2 cut(s) 143, 630
BstV2I GAAGAC 2 cut(s) 69, 691
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
BsuI GTATCC 1 cut(s) 535
BsuRI GGCC 1 cut(s) 452
BtgI CCRYGG 1 cut(s) 638
BtsCI GGATG 2 cut(s) 255, 370
BtsIMutI CAGTG 2 cut(s) 239, 347
Cac8I GCNNGC 1 cut(s) 495
CciI TCATGA 1 cut(s) 690
Cfr13I GGNCC 1 cut(s) 500
Csp6I GTAC 4 cut(s) 28, 237, 295, 677
CviAII CATG 5 cut(s) 272, 454, 616, 639, 691
CviQI GTAC 4 cut(s) 28, 237, 295, 677
DdeI CTNAG 1 cut(s) 680
DpnI GATC 1 cut(s) 147
DpnII GATC 1 cut(s) 145
DraIII CACNNNGTG 1 cut(s) 351
DrdI GACNNNNNNGTC 1 cut(s) 611
DseDI GACNNNNNNGTC 1 cut(s) 611
EaeI YGGCCR 1 cut(s) 450
Ecl136II GAGCTC 1 cut(s) 526
Eco130I CCWWGG 2 cut(s) 505, 638
Eco24I GRGCYC 1 cut(s) 528
Eco47I GGWCC 1 cut(s) 500
Eco53kI GAGCTC 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 171
Eco72I CACGTG 1 cut(s) 60
EcoICRI GAGCTC 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 8, 148
EcoT14I CCWWGG 2 cut(s) 505, 638
EcoT22I ATGCAT 1 cut(s) 733
EcoT38I GRGCYC 1 cut(s) 528
ErhI CCWWGG 2 cut(s) 505, 638
FaeI CATG 5 cut(s) 275, 457, 619, 642, 694
FaqI GGGAC 4 cut(s) 93, 237, 486, 642
FatI CATG 5 cut(s) 271, 453, 615, 638, 690
FauNDI CATATG 1 cut(s) 246
Fnu4HI GCNGC 2 cut(s) 157, 644
FokI GGATG 2 cut(s) 242, 357
FriOI GRGCYC 1 cut(s) 528
Fsp4HI GCNGC 2 cut(s) 157, 644
FspBI CTAG 1 cut(s) 41
GluI GCNGC 2 cut(s) 157, 644
GsuI CTGGAG 2 cut(s) 319, 609
HaeIII GGCC 1 cut(s) 452
HapII CCGG 1 cut(s) 204
Hin1II CATG 5 cut(s) 275, 457, 619, 642, 694
HinfI GANTC 4 cut(s) 261, 355, 629, 666
HpaII CCGG 1 cut(s) 204
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 4 cut(s) 132, 260, 375, 523
Hpy188III TCNNGA 3 cut(s) 100, 626, 691
Hpy8I GTNNAC 1 cut(s) 237
HpyAV CCTTC 2 cut(s) 91, 579
HpyCH4III ACNGT 3 cut(s) 351, 611, 697
HpyCH4IV ACGT 4 cut(s) 15, 26, 59, 513
HpyCH4V TGCA 4 cut(s) 24, 367, 517, 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 277, 523
HpyF3I CTNAG 1 cut(s) 680
HpySE526I ACGT 4 cut(s) 15, 26, 59, 513
Hsp92II CATG 5 cut(s) 275, 457, 619, 642, 694
Kzo9I GATC 1 cut(s) 145
LmnI GCTCC 1 cut(s) 531
Lsp1109I GCAGC 2 cut(s) 143, 630
MaeI CTAG 1 cut(s) 41
MaeII ACGT 4 cut(s) 15, 26, 59, 513
MaeIII GTNAC 2 cut(s) 16, 310
MalI GATC 1 cut(s) 147
MboI GATC 1 cut(s) 145
MboII GAAGA 2 cut(s) 69, 691
MflI RGATCY 1 cut(s) 145
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 7 cut(s) 35, 283, 315, 322, 328, 385, 409
MlyI GAGTC 1 cut(s) 270
MnlI CCTC 5 cut(s) 234, 294, 497, 591, 633
Mph1103I ATGCAT 1 cut(s) 733
MseI TTAA 3 cut(s) 276, 422, 440
MslI CAYNNNNRTG 2 cut(s) 58, 270
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 3 cut(s) 10, 150, 205
MvaI CCWGG 2 cut(s) 10, 150
MwoI GCNNNNNNNGC 2 cut(s) 277, 523
NciI CCSGG 1 cut(s) 205
NcoI CCATGG 1 cut(s) 638
NdeI CATATG 1 cut(s) 246
NdeII GATC 1 cut(s) 145
NlaIII CATG 5 cut(s) 275, 457, 619, 642, 694
NlaIV GGNNCC 2 cut(s) 202, 502
NmuCI GTSAC 1 cut(s) 310
NsiI ATGCAT 1 cut(s) 733
NspI RCATGY 1 cut(s) 275
NspV TTCGAA 1 cut(s) 535
OliI CACNNNNGTG 1 cut(s) 58
PagI TCATGA 1 cut(s) 690
PfeI GAWTC 3 cut(s) 355, 629, 666
PflMI CCANNNNNTGG 1 cut(s) 459
PfoI TCCNGGA 2 cut(s) 148, 203
PkrI GCNGC 2 cut(s) 158, 645
PleI GAGTC 1 cut(s) 269
PmaCI CACGTG 1 cut(s) 60
PmlI CACGTG 1 cut(s) 60
PpsI GAGTC 1 cut(s) 269
Ppu21I YACGTR 2 cut(s) 27, 60
PsiI TTATAA 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 528
Psp1406I AACGTT 1 cut(s) 15
Psp6I CCWGG 2 cut(s) 8, 148
PspCI CACGTG 1 cut(s) 60
PspGI CCWGG 2 cut(s) 8, 148
PspN4I GGNNCC 2 cut(s) 202, 502
PspPI GGNCC 1 cut(s) 500
PsuI RGATCY 1 cut(s) 145
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 4 cut(s) 29, 238, 296, 678
RsaNI GTAC 4 cut(s) 28, 237, 295, 677
RseI CAYNNNNRTG 2 cut(s) 58, 270
SacI GAGCTC 1 cut(s) 528
SaqAI TTAA 3 cut(s) 276, 422, 440
SatI GCNGC 2 cut(s) 157, 644
Sau3AI GATC 1 cut(s) 145
Sau96I GGNCC 1 cut(s) 500
ScaI AGTACT 1 cut(s) 296
SchI GAGTC 1 cut(s) 270
ScrFI CCNGG 3 cut(s) 10, 150, 205
SduI GDGCHC 1 cut(s) 528
SfcI CTRYAG 1 cut(s) 141
SfuI TTCGAA 1 cut(s) 535
SinI GGWCC 1 cut(s) 500
SmiMI CAYNNNNRTG 2 cut(s) 58, 270
Sse9I AATT 7 cut(s) 35, 283, 315, 322, 328, 385, 409
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 528
StyD4I CCNGG 3 cut(s) 8, 148, 203
StyI CCWWGG 2 cut(s) 505, 638
TaaI ACNGT 3 cut(s) 351, 611, 697
TaiI ACGT 4 cut(s) 18, 29, 62, 516
TaqI TCGA 2 cut(s) 535, 583
TasI AATT 7 cut(s) 35, 283, 315, 322, 328, 385, 409
TatI WGTACW 1 cut(s) 294
TfiI GAWTC 3 cut(s) 355, 629, 666
Tru1I TTAA 3 cut(s) 276, 422, 440
Tru9I TTAA 3 cut(s) 276, 422, 440
TscAI CASTG 2 cut(s) 239, 354
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 2 cut(s) 156, 643
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 1 cut(s) 353
TspGWI ACGGA 1 cut(s) 615
TspRI CASTG 2 cut(s) 239, 354
Van91I CCANNNNNTGG 1 cut(s) 459
VpaK11BI GGWCC 1 cut(s) 500
XapI RAATTY 1 cut(s) 322
XceI RCATGY 1 cut(s) 275
XspI CTAG 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 296
Zsp2I ATGCAT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.