Rh7AG058700

COBRA-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0013790
Physical Location & Seq
Forward (+)
4040167 .. 4044125
3959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG058700.1

Sequence Viewer

Length: 1176 bp
ATGTTCAACTTCCAGCAGTATCGCCACATACAAGCGCCAGGTTGGACGTTAGGATGGACATGGGCAAAGAAGGAAGTAATCTGGAGCATGGTGGGAGCGCAAACCACCGAGCAAGGGGACTGTTCAAGATACAAAGGCAATGTACCACATTGCTGTAAGAAGGATCCCACAGTTGTGGATTTGTTACCCGGAACTCCTTATAACCAGCAGATTGCAAATTGCTGCAAAGCAGGGGTTATGCAATCATATATCCAAGACCCAGCGAATGCTGTGAGTTCATTCCAGATCAGTGTGGGTGCTGCAGGAACCACTAACAAGACTGTTAGACTTCCTAAAAACTTCACATTGAAAGCACCAGGGCCTGGATATTCATGTGGGCGAGCAGCGATTGTCAAGCCAACCAGATATTTAACAGCAGATAAAAGAAGAATGACCCAAGCCATGATGACCTGGAATGTTACTTGCACATACTCTCAATTCCTAGCTCAAAAGACACCTACTTGTTGTGTTTCTCTCTCCTCCTTCTATAATGACACCATAGTAAACTGTCCTACTTGTGCATGTGGTTGTGAGAACAACAGTACAGATCCCGGGAGCTGCGTAGAGCCAGACTCTCCGTACTTAGCTTCAGCTGTAGCAGGTTCTGGAAAAGCTACTAATTCTCCGTTGGTCCAGTGTACCAGCCATATGTGTCCAGTGAGAGTCCATTGGCATGTGAAGCTCAATTACAAGGAGTACTGGAGGGTGAAAGTTACAGTTACAAATTTCAATTACAGAATGAACTACACTCTCTGGAATATTGTTGTTCAACATCCCAACTTCGATAATTTGACCAAGATTTTCAGCTTTAATTACAAGGACTTGACTCCTTATGCTGGCTTGAATGATACTGCAATGTTGTGGGGAGTCAAGTTTTATAATGACTTGCTCATGCAAGCTGGTCCTCTTGGAAATGCGCAGTCAGAGCTTCTATTTCGGAAAGATGCGTCAACTTTTACTTTTGAAAAGGGATGGGCTTTCCCTCGAAGGATTTATTTTAACGGTGATAACTGTGTCATGCCACCTCCAGATGCTTATCCGTGGTTGCCAAATGCCAGTCCTAGACCAACCATTTCCCTACTTAGTCCGGTTATAACTATCTTGGCATCTCTGGTATTCTTATTGTCTTATGCGTAG

Protein Analysis

391

Amino Acids

43.67

Weight (kDa)

8.99

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COBRA PF04833 1 - 158 9.3e-66 COBRA-like protein
COB_C PF25079 167 - 360 3.8e-62 COBRA family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60920 AT5G60920
fragaria_vesca FvH4_5g13550
malus_domestica MD06G1203700.v1.1 MD14G1214800.v1.1
prunus_persica Prupe.5G207800_v2.0.a1
pyrus_communis pycom06g18190 pycom14g17790
rosa_chinensis RchiOBHm_Chr7g0183381
rosa_laevigata RLG00000005051
rosa_multiflora Rmu_sc0000951.1_g000012
rosa_roxburghii Rroxscaffold_3G00270490
rosa_rugosa Rorug06G0457600
rosa_samantha Rh7AG058700 Rh7BG058700 Rh7DG058400
rosa_wichuraiana Rw7G004830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 201, 918, 1133
Acc16I TGCGCA 1 cut(s) 957
Acc36I ACCTGC 1 cut(s) 629
AccB7I CCANNNNNTGG 1 cut(s) 362
AclWI GGATC 3 cut(s) 158, 171, 581
AcsI RAATTY 1 cut(s) 763
AcuI CTGAAG 1 cut(s) 612
AfaI GTAC 5 cut(s) 144, 583, 620, 679, 737
AfiI CCNNNNNNNGG 3 cut(s) 114, 362, 875
AgsI TTSAA 7 cut(s) 7, 126, 349, 769, 809, 883, 1004
AjnI CCWGG 4 cut(s) 37, 355, 361, 449
AleI CACNNNNGTG 1 cut(s) 173
AluBI AGCT 9 cut(s) 485, 597, 626, 632, 653, 721, 846, 938, 967
AluI AGCT 9 cut(s) 485, 597, 626, 632, 653, 721, 846, 938, 967
AlwI GGATC 3 cut(s) 158, 171, 581
AlwNI CAGNNNCTG 2 cut(s) 362, 644
Ama87I CYCGRG 1 cut(s) 590
AoxI GGCC 1 cut(s) 359
ApeKI GCWGC 4 cut(s) 222, 299, 383, 597
ApoI RAATTY 1 cut(s) 763
AspLEI GCGC 3 cut(s) 37, 100, 958
AspS9I GGNCC 3 cut(s) 359, 670, 941
AsuC2I CCSGG 3 cut(s) 189, 591, 592
AsuHPI GGTGA 2 cut(s) 757, 1055
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 2 cut(s) 670, 941
BamHI GGATCC 1 cut(s) 163
BarI GAAGNNNNNNTAC 2 cut(s) 710, 742
BbvI GCAGC 4 cut(s) 209, 286, 395, 584
BccI CCATC 2 cut(s) 48, 1005
BciT130I CCWGG 4 cut(s) 39, 357, 363, 451
BcnI CCSGG 3 cut(s) 189, 591, 592
BfaI CTAG 2 cut(s) 482, 1101
BfmI CTRYAG 2 cut(s) 300, 633
BfoI RGCGCY 1 cut(s) 38
BfuAI ACCTGC 1 cut(s) 629
BisI GCNGC 4 cut(s) 223, 300, 384, 598
BlsI GCNGC 4 cut(s) 224, 301, 385, 599
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 7 cut(s) 39, 189, 357, 363, 451, 591, 592
Bme18I GGWCC 2 cut(s) 670, 941
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 3 cut(s) 359, 670, 941
BmiI GGNNCC 2 cut(s) 165, 307
BmrFI CCNGG 7 cut(s) 39, 189, 357, 363, 451, 591, 592
BmsI GCATC 3 cut(s) 973, 1060, 1154
BplI GAGNNNNNCTC 2 cut(s) 596, 628
BpmI CTGGAG 3 cut(s) 103, 760, 1050
BpuMI CCSGG 3 cut(s) 189, 591, 592
BsaBI GATNNNNATC 1 cut(s) 1074
BsaJI CCNNGG 3 cut(s) 356, 590, 1079
BsaWI WCCGGW 1 cut(s) 1126
BsaXI ACNNNNNCTCC 2 cut(s) 646, 676
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 362, 875
Bse1I ACTGG 4 cut(s) 673, 695, 743, 1095
Bse3DI GCAATG 3 cut(s) 145, 148, 900
Bse8I GATNNNNATC 1 cut(s) 1074
BseBI CCWGG 4 cut(s) 39, 357, 363, 451
BseDI CCNNGG 3 cut(s) 356, 590, 1079
BseGI GGATG 3 cut(s) 59, 811, 1016
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 3 cut(s) 114, 362, 875
BseMI GCAATG 3 cut(s) 145, 148, 900
BseNI ACTGG 4 cut(s) 673, 695, 743, 1095
BseRI GAGGAG 1 cut(s) 508
BseXI GCAGC 4 cut(s) 209, 286, 395, 584
BseYI CCCAGC 1 cut(s) 259
BshFI GGCC 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 3 cut(s) 189, 591, 1127
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 3 cut(s) 114, 362, 875
BsmFI GGGAC 1 cut(s) 131
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 1 cut(s) 361
BsoBI CYCGRG 1 cut(s) 590
Bsp143I GATC 3 cut(s) 163, 285, 586
BspANI GGCC 1 cut(s) 361
BspLI GGNNCC 2 cut(s) 165, 307
BspMAI CTGCAG 1 cut(s) 304
BspMI ACCTGC 1 cut(s) 629
BspPI GGATC 3 cut(s) 158, 171, 581
BsrDI GCAATG 3 cut(s) 145, 148, 900
BsrI ACTGG 4 cut(s) 673, 695, 743, 1095
BssECI CCNNGG 3 cut(s) 356, 590, 1079
BssMI GATC 3 cut(s) 163, 285, 586
Bst2UI CCWGG 4 cut(s) 39, 357, 363, 451
Bst4CI ACNGT 8 cut(s) 122, 172, 322, 548, 581, 757, 1043, 1052
BstC8I GCNNGC 3 cut(s) 381, 877, 936
BstDEI CTNAG 2 cut(s) 622, 1121
BstDSI CCRYGG 1 cut(s) 1079
BstF5I GGATG 3 cut(s) 59, 811, 1016
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 3 cut(s) 37, 100, 958
BstKTI GATC 3 cut(s) 166, 288, 589
BstMBI GATC 3 cut(s) 163, 285, 586
BstMWI GCNNNNNNNGC 2 cut(s) 718, 964
BstNI CCWGG 4 cut(s) 39, 357, 363, 451
BstNSI RCATGY 2 cut(s) 564, 716
BstSCI CCNGG 7 cut(s) 37, 187, 355, 361, 449, 589, 590
BstSFI CTRYAG 2 cut(s) 300, 633
BstV1I GCAGC 4 cut(s) 209, 286, 395, 584
BstX2I RGATCY 2 cut(s) 163, 586
BstXI CCANNNNNNTGG 1 cut(s) 175
BstYI RGATCY 2 cut(s) 163, 586
BsuRI GGCC 1 cut(s) 361
BtgI CCRYGG 1 cut(s) 1079
BtsCI GGATG 3 cut(s) 59, 811, 1016
BtsIMutI CAGTG 3 cut(s) 295, 680, 702
BveI ACCTGC 1 cut(s) 629
Cac8I GCNNGC 3 cut(s) 381, 877, 936
CaiI CAGNNNCTG 2 cut(s) 362, 644
CfoI GCGC 3 cut(s) 37, 100, 958
Cfr13I GGNCC 3 cut(s) 359, 670, 941
Cfr9I CCCGGG 1 cut(s) 590
CseI GACGC 1 cut(s) 975
Csp6I GTAC 5 cut(s) 143, 582, 619, 678, 736
CviAII CATG 8 cut(s) 60, 88, 372, 442, 561, 713, 931, 1057
CviQI GTAC 5 cut(s) 143, 582, 619, 678, 736
DdeI CTNAG 2 cut(s) 622, 1121
DpnI GATC 3 cut(s) 165, 287, 588
DpnII GATC 3 cut(s) 163, 285, 586
Eco47I GGWCC 2 cut(s) 670, 941
Eco57I CTGAAG 1 cut(s) 612
Eco88I CYCGRG 1 cut(s) 590
EcoO109I RGGNCCY 1 cut(s) 359
EcoRII CCWGG 4 cut(s) 37, 355, 361, 449
FaeI CATG 8 cut(s) 63, 91, 375, 445, 564, 716, 934, 1060
FaqI GGGAC 1 cut(s) 131
FatI CATG 8 cut(s) 59, 87, 371, 441, 560, 712, 930, 1056
FauNDI CATATG 1 cut(s) 687
Fnu4HI GCNGC 4 cut(s) 223, 300, 384, 598
FokI GGATG 3 cut(s) 66, 798, 1023
Fsp4HI GCNGC 4 cut(s) 223, 300, 384, 598
FspBI CTAG 2 cut(s) 482, 1101
FspI TGCGCA 1 cut(s) 957
GlaI GCGC 3 cut(s) 36, 99, 957
GluI GCNGC 4 cut(s) 223, 300, 384, 598
GsaI CCCAGC 1 cut(s) 263
GsuI CTGGAG 3 cut(s) 103, 760, 1050
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 1 cut(s) 361
HapII CCGG 3 cut(s) 189, 591, 1127
HgaI GACGC 1 cut(s) 975
HhaI GCGC 3 cut(s) 37, 100, 958
Hin1II CATG 8 cut(s) 63, 91, 375, 445, 564, 716, 934, 1060
Hin6I GCGC 3 cut(s) 35, 98, 956
HinP1I GCGC 3 cut(s) 35, 98, 956
HincII GTYRAC 1 cut(s) 990
HindII GTYRAC 1 cut(s) 990
HinfI GANTC 4 cut(s) 611, 702, 865, 906
HpaII CCGG 3 cut(s) 189, 591, 1127
HphI GGTGA 2 cut(s) 757, 1055
Hpy166II GTNNAC 3 cut(s) 544, 678, 990
Hpy188I TCNGA 2 cut(s) 964, 978
Hpy188III TCNNGA 6 cut(s) 82, 126, 283, 645, 793, 1067
Hpy8I GTNNAC 3 cut(s) 544, 678, 990
HpyAV CCTTC 4 cut(s) 64, 154, 532, 1020
HpyCH4III ACNGT 8 cut(s) 122, 172, 322, 548, 581, 757, 1043, 1052
HpyCH4IV ACGT 1 cut(s) 47
HpyCH4V TGCA 8 cut(s) 215, 225, 241, 302, 465, 560, 893, 934
HpyF10VI GCNNNNNNNGC 2 cut(s) 718, 964
HpyF3I CTNAG 2 cut(s) 622, 1121
HpySE526I ACGT 1 cut(s) 47
Hsp92II CATG 8 cut(s) 63, 91, 375, 445, 564, 716, 934, 1060
HspAI GCGC 3 cut(s) 35, 98, 956
Kzo9I GATC 3 cut(s) 163, 285, 586
LmnI GCTCC 3 cut(s) 84, 95, 594
Lsp1109I GCAGC 4 cut(s) 209, 286, 395, 584
LweI GCATC 3 cut(s) 973, 1060, 1154
MaeI CTAG 2 cut(s) 482, 1101
MaeII ACGT 1 cut(s) 47
MaeIII GTNAC 4 cut(s) 183, 457, 751, 757
MalI GATC 3 cut(s) 165, 287, 588
MboI GATC 3 cut(s) 163, 285, 586
MboII GAAGA 1 cut(s) 438
MflI RGATCY 2 cut(s) 163, 586
MluCI AATT 8 cut(s) 217, 476, 658, 724, 763, 769, 826, 850
MlyI GAGTC 4 cut(s) 605, 711, 859, 915
MmeI TCCRAC 1 cut(s) 23
MnlI CCTC 5 cut(s) 529, 735, 954, 1032, 1074
MseI TTAA 3 cut(s) 410, 849, 1038
MslI CAYNNNNRTG 2 cut(s) 173, 711
MspA1I CMGCKG 1 cut(s) 632
MspI CCGG 3 cut(s) 189, 591, 1127
MspR9I CCNGG 7 cut(s) 39, 189, 357, 363, 451, 591, 592
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 4 cut(s) 39, 357, 363, 451
MwoI GCNNNNNNNGC 2 cut(s) 718, 964
NciI CCSGG 3 cut(s) 189, 591, 592
NdeI CATATG 1 cut(s) 687
NdeII GATC 3 cut(s) 163, 285, 586
NlaIII CATG 8 cut(s) 63, 91, 375, 445, 564, 716, 934, 1060
NlaIV GGNNCC 2 cut(s) 165, 307
NsbI TGCGCA 1 cut(s) 957
NspI RCATGY 2 cut(s) 564, 716
OliI CACNNNNGTG 1 cut(s) 173
PctI GAATGC 1 cut(s) 271
PflMI CCANNNNNTGG 1 cut(s) 362
PkrI GCNGC 4 cut(s) 224, 301, 385, 599
PleI GAGTC 4 cut(s) 605, 710, 859, 914
PpsI GAGTC 4 cut(s) 605, 710, 859, 914
PsiI TTATAA 3 cut(s) 201, 918, 1133
Psp6I CCWGG 4 cut(s) 37, 355, 361, 449
PspFI CCCAGC 1 cut(s) 259
PspGI CCWGG 4 cut(s) 37, 355, 361, 449
PspN4I GGNNCC 2 cut(s) 165, 307
PspPI GGNCC 3 cut(s) 359, 670, 941
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 2 cut(s) 362, 644
PsuI RGATCY 2 cut(s) 163, 586
PvuII CAGCTG 1 cut(s) 632
RsaI GTAC 5 cut(s) 144, 583, 620, 679, 737
RsaNI GTAC 5 cut(s) 143, 582, 619, 678, 736
RseI CAYNNNNRTG 2 cut(s) 173, 711
SaqAI TTAA 3 cut(s) 410, 849, 1038
SatI GCNGC 4 cut(s) 223, 300, 384, 598
Sau3AI GATC 3 cut(s) 163, 285, 586
Sau96I GGNCC 3 cut(s) 359, 670, 941
ScaI AGTACT 1 cut(s) 737
SchI GAGTC 4 cut(s) 605, 711, 859, 915
ScrFI CCNGG 7 cut(s) 39, 189, 357, 363, 451, 591, 592
SfaNI GCATC 3 cut(s) 973, 1060, 1154
SfcI CTRYAG 2 cut(s) 300, 633
SinI GGWCC 2 cut(s) 670, 941
SmaI CCCGGG 1 cut(s) 592
SmiMI CAYNNNNRTG 2 cut(s) 173, 711
Sse9I AATT 8 cut(s) 217, 476, 658, 724, 763, 769, 826, 850
SspI AATATT 1 cut(s) 799
SspMI CTAG 2 cut(s) 482, 1101
StyD4I CCNGG 7 cut(s) 37, 187, 355, 361, 449, 589, 590
TaaI ACNGT 8 cut(s) 122, 172, 322, 548, 581, 757, 1043, 1052
TaiI ACGT 1 cut(s) 50
TaqI TCGA 2 cut(s) 822, 1024
TasI AATT 8 cut(s) 217, 476, 658, 724, 763, 769, 826, 850
TatI WGTACW 2 cut(s) 581, 735
Tru1I TTAA 3 cut(s) 410, 849, 1038
Tru9I TTAA 3 cut(s) 410, 849, 1038
TscAI CASTG 3 cut(s) 295, 680, 702
TseI GCWGC 4 cut(s) 222, 299, 383, 597
TspDTI ATGAA 3 cut(s) 267, 360, 794
TspGWI ACGGA 3 cut(s) 606, 654, 1068
TspMI CCCGGG 1 cut(s) 590
TspRI CASTG 3 cut(s) 295, 680, 702
Van91I CCANNNNNTGG 1 cut(s) 362
VpaK11BI GGWCC 2 cut(s) 670, 941
XapI RAATTY 1 cut(s) 763
XceI RCATGY 2 cut(s) 564, 716
XmaI CCCGGG 1 cut(s) 590
XspI CTAG 2 cut(s) 482, 1101
ZrmI AGTACT 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.