pycom06g19640

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012352
Physical Location & Seq
Forward (+)
22986232 .. 22987368
1137 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 681 bp
ATGTCTAGAAGGCCGGGATTAGTATATTCGTCGGATTCTGATGACGATGGAGTAATCCACGGTGCAAAGCTCTTCGGACGTGAAAGGCCGATATATCATGTTCTTGGAGGTGGAAAAGTGGCGGATGTGTTGTTGTGGAGGAATATAAATGTATCAGCTGGGCTTTTAATTGCTATGACGGTAATCTGGTTTCTCTTCGAAGTTGTTGAGTACAATTTCGTGACGCTCGTCTGTCATATCTCGATTACCGCATTGTTGGTAATCTTCATATGGCGCATGGCTGCAGAAATATTCAAATTTCCGCCGCCTATGGTACCTCAAATGATATTACATGAAAACACATTCAAACAAGTAGCTTCCACTGTCCATAAAGGATTCAACCACTTCTTATCAAGAGTTCTTGATAGTGCATGTGGAAGAGACTTCCCATTCTTCTTCTTGACAATCGTTTGTCTCTATATTTTGTCTGTGATCGGAAACTATTTCAGCTTTCTGAATCTTCTTTACTTGGGGTTCATCTGCTTGGGAACGCTACCATTCCTCTACGAGCGATTTGAGGAAGACGTTGATCGATTAGCCGGAAAAATCAGCCGAGAGATAAAGAGATCGTACAGAAAGTTTGATTCTCAATTTCTCAACAAAATTCCTAGAGGTCCAGTGAAAGACAAGAAAAACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

227

Amino Acids

26.01

Weight (kDa)

9.3

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 40 - 195 4.9e-48 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012352)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18260
fragaria_vesca FvH4_5g16680 FvH4_5g16680
malus_domestica MD06G1220200.v1.1 MD14G1229700.v1.1
prunus_persica Prupe.5G224500_v2.0.a1
pyrus_communis pycom06g19640 pycom14g19120
rosa_chinensis RchiOBHm_Chr7g0179441
rosa_laevigata RLG00000005356
rosa_multiflora Rmu_co8409275.1_g000001
rosa_roxburghii Rroxscaffold_3G00274020
rosa_rugosa Rorug06G0424200 Rorug06G0424200
rosa_samantha Rh7AG023800 Rh7BG023400 Rh7CG024800 Rh7DG024300
rosa_wichuraiana Rw7G001930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 313
AciI CCGC 4 cut(s) 122, 249, 302, 305
AcsI RAATTY 2 cut(s) 296, 642
AfaI GTAC 3 cut(s) 212, 315, 611
AgsI TTSAA 3 cut(s) 295, 346, 379
AjiI CACGTC 1 cut(s) 80
AluBI AGCT 4 cut(s) 70, 158, 356, 489
AluI AGCT 4 cut(s) 70, 158, 356, 489
Alw26I GTCTC 2 cut(s) 414, 458
AoxI GGCC 2 cut(s) 11, 86
ApeKI GCWGC 1 cut(s) 281
ApoI RAATTY 2 cut(s) 296, 642
Asp718I GGTACC 1 cut(s) 313
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 653
AsuC2I CCSGG 1 cut(s) 15
AsuII TTCGAA 1 cut(s) 198
AvaII GGWCC 1 cut(s) 653
BanI GGYRCC 1 cut(s) 313
BbsI GAAGAC 1 cut(s) 567
BbvI GCAGC 1 cut(s) 268
BccI CCATC 1 cut(s) 41
BcnI CCSGG 1 cut(s) 15
BcoDI GTCTC 2 cut(s) 414, 458
BfaI CTAG 2 cut(s) 6, 648
BfmI CTRYAG 1 cut(s) 282
BisI GCNGC 2 cut(s) 282, 305
BlsI GCNGC 2 cut(s) 283, 306
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 1 cut(s) 653
BmgBI CACGTC 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 653
BmiI GGNNCC 1 cut(s) 315
BmrFI CCNGG 1 cut(s) 15
BoxI GACNNNNGTC 1 cut(s) 227
BpiI GAAGAC 1 cut(s) 567
Bpu14I TTCGAA 1 cut(s) 198
BpuMI CCSGG 1 cut(s) 15
Bsa29I ATCGAT 1 cut(s) 571
BsaJI CCNNGG 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 656
BseCI ATCGAT 1 cut(s) 571
BseDI CCNNGG 1 cut(s) 58
BseGI GGATG 1 cut(s) 130
BseNI ACTGG 1 cut(s) 656
BseXI GCAGC 1 cut(s) 268
BseYI CCCAGC 1 cut(s) 158
BshFI GGCC 2 cut(s) 13, 88
BshNI GGYRCC 1 cut(s) 313
BshVI ATCGAT 1 cut(s) 571
BsiSI CCGG 2 cut(s) 14, 579
BsmAI GTCTC 2 cut(s) 414, 458
BsnI GGCC 2 cut(s) 13, 88
Bsp119I TTCGAA 1 cut(s) 198
Bsp143I GATC 3 cut(s) 471, 568, 605
BspACI CCGC 4 cut(s) 122, 249, 302, 305
BspANI GGCC 2 cut(s) 13, 88
BspDI ATCGAT 1 cut(s) 571
BspLI GGNNCC 1 cut(s) 315
BspMAI CTGCAG 1 cut(s) 286
BspQI GCTCTTC 1 cut(s) 77
BspT104I TTCGAA 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 313
BsrI ACTGG 1 cut(s) 656
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 3 cut(s) 471, 568, 605
Bst4CI ACNGT 3 cut(s) 62, 181, 364
Bst6I CTCTTC 3 cut(s) 77, 200, 412
BstBI TTCGAA 1 cut(s) 198
BstDSI CCRYGG 1 cut(s) 58
BstF5I GGATG 1 cut(s) 130
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 3 cut(s) 474, 571, 608
BstMAI GTCTC 2 cut(s) 414, 458
BstMBI GATC 3 cut(s) 471, 568, 605
BstNSI RCATGY 1 cut(s) 414
BstPAI GACNNNNGTC 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 268
BstV2I GAAGAC 1 cut(s) 567
Bsu15I ATCGAT 1 cut(s) 571
BsuRI GGCC 2 cut(s) 13, 88
BsuTUI ATCGAT 1 cut(s) 571
BtgI CCRYGG 1 cut(s) 58
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 1 cut(s) 130
BtsIMutI CAGTG 2 cut(s) 360, 663
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 653
ClaI ATCGAT 1 cut(s) 571
CseI GACGC 1 cut(s) 232
Csp6I GTAC 3 cut(s) 211, 314, 610
CviAII CATG 4 cut(s) 98, 277, 332, 411
CviQI GTAC 3 cut(s) 211, 314, 610
DpnI GATC 3 cut(s) 473, 570, 607
DpnII GATC 3 cut(s) 471, 568, 605
Eam1104I CTCTTC 3 cut(s) 77, 200, 412
EarI CTCTTC 3 cut(s) 77, 200, 412
EciI GGCGGA 2 cut(s) 137, 291
Eco47I GGWCC 1 cut(s) 653
FaeI CATG 4 cut(s) 101, 280, 335, 414
FatI CATG 4 cut(s) 97, 276, 331, 410
FauNDI CATATG 1 cut(s) 269
Fnu4HI GCNGC 2 cut(s) 282, 305
FokI GGATG 1 cut(s) 137
Fsp4HI GCNGC 2 cut(s) 282, 305
FspBI CTAG 2 cut(s) 6, 648
GlaI GCGC 1 cut(s) 275
GluI GCNGC 2 cut(s) 282, 305
GsaI CCCAGC 1 cut(s) 162
HaeIII GGCC 2 cut(s) 13, 88
HapII CCGG 2 cut(s) 14, 579
HgaI GACGC 1 cut(s) 232
HhaI GCGC 1 cut(s) 276
Hin1II CATG 4 cut(s) 101, 280, 335, 414
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HinfI GANTC 4 cut(s) 35, 375, 496, 623
HpaII CCGG 2 cut(s) 14, 579
Hpy188I TCNGA 5 cut(s) 34, 40, 77, 476, 495
Hpy188III TCNNGA 6 cut(s) 6, 220, 241, 393, 401, 439
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 1 cut(s) 3
HpyCH4III ACNGT 3 cut(s) 62, 181, 364
HpyCH4IV ACGT 2 cut(s) 79, 564
HpyCH4V TGCA 3 cut(s) 65, 284, 410
HpySE526I ACGT 2 cut(s) 79, 564
Hsp92II CATG 4 cut(s) 101, 280, 335, 414
HspAI GCGC 1 cut(s) 274
KpnI GGTACC 1 cut(s) 317
Kzo9I GATC 3 cut(s) 471, 568, 605
LguI GCTCTTC 1 cut(s) 77
LpnPI CCDG 5 cut(s) 27, 144, 172, 592, 669
Lsp1109I GCAGC 1 cut(s) 268
MaeI CTAG 2 cut(s) 6, 648
MaeII ACGT 2 cut(s) 79, 564
MaeIII GTNAC 1 cut(s) 220
MalI GATC 3 cut(s) 473, 570, 607
MboI GATC 3 cut(s) 471, 568, 605
MboII GAAGA 8 cut(s) 64, 187, 256, 424, 427, 429, 491, 572
MluCI AATT 5 cut(s) 168, 214, 296, 629, 642
MmeI TCCRAC 1 cut(s) 12
MnlI CCTC 6 cut(s) 101, 132, 327, 550, 551, 644
MseI TTAA 1 cut(s) 167
MspA1I CMGCKG 1 cut(s) 158
MspI CCGG 2 cut(s) 14, 579
MspR9I CCNGG 1 cut(s) 15
NciI CCSGG 1 cut(s) 15
NdeI CATATG 1 cut(s) 269
NdeII GATC 3 cut(s) 471, 568, 605
NlaIII CATG 4 cut(s) 101, 280, 335, 414
NlaIV GGNNCC 1 cut(s) 315
NmeAIII GCCGAG 1 cut(s) 617
NmuCI GTSAC 1 cut(s) 220
NspI RCATGY 1 cut(s) 414
NspV TTCGAA 1 cut(s) 198
PciSI GCTCTTC 1 cut(s) 77
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PfeI GAWTC 4 cut(s) 35, 375, 496, 623
PkrI GCNGC 2 cut(s) 283, 306
PshAI GACNNNNGTC 1 cut(s) 227
PspFI CCCAGC 1 cut(s) 158
PspN4I GGNNCC 1 cut(s) 315
PspPI GGNCC 1 cut(s) 653
PstI CTGCAG 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 158
RsaI GTAC 3 cut(s) 212, 315, 611
RsaNI GTAC 3 cut(s) 211, 314, 610
SapI GCTCTTC 1 cut(s) 77
SaqAI TTAA 1 cut(s) 167
SatI GCNGC 2 cut(s) 282, 305
Sau3AI GATC 3 cut(s) 471, 568, 605
Sau96I GGNCC 1 cut(s) 653
ScrFI CCNGG 1 cut(s) 15
SetI ASST 9 cut(s) 72, 82, 112, 160, 319, 358, 491, 567, 655
SfcI CTRYAG 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 198
SinI GGWCC 1 cut(s) 653
Sse9I AATT 5 cut(s) 168, 214, 296, 629, 642
SsiI CCGC 4 cut(s) 122, 249, 302, 305
SspI AATATT 1 cut(s) 291
SspMI CTAG 2 cut(s) 6, 648
StyD4I CCNGG 1 cut(s) 13
TaaI ACNGT 3 cut(s) 62, 181, 364
TaiI ACGT 2 cut(s) 82, 567
TaqI TCGA 3 cut(s) 198, 242, 571
TasI AATT 5 cut(s) 168, 214, 296, 629, 642
TatI WGTACW 1 cut(s) 210
TauI GCSGC 1 cut(s) 307
TfiI GAWTC 4 cut(s) 35, 375, 496, 623
Tru1I TTAA 1 cut(s) 167
Tru9I TTAA 1 cut(s) 167
TscAI CASTG 2 cut(s) 367, 663
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 281
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 3 cut(s) 256, 348, 505
TspRI CASTG 2 cut(s) 367, 663
VpaK11BI GGWCC 1 cut(s) 653
XapI RAATTY 2 cut(s) 296, 642
XbaI TCTAGA 1 cut(s) 5
XceI RCATGY 1 cut(s) 414
XspI CTAG 2 cut(s) 6, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.