pycom06g20290

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014745
Physical Location & Seq
Forward (+)
23384345 .. 23386646
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1446 bp
ATGGCATGCCAATCATCAACAATATGGAGAACAAAAGCTGAATCTGCTTCCAAAATTTCAAGCTGCTTTAACAACAGCTCCTCCATGCTCCTCTTCTCCTCCGCCATTCGCAACAAAAGCCGCCGTTTCCTCACTTGTTCGTTAGACCCCATCTCCACTTCATCAACAACATCTTCTTCTTCAGCCTCACAGAAAGCTAGTTGCAAGAACATAACAAACAGAAAGTCTCTTGAAGTACAAAGAACCAAGGATGGGGCTTCCTCGGTTTTGCATGGCGATGAGCGTAGGCAATCCGATAAGAAGATTAAGAAGAGCAAGAAGGATGAGACTAAGAATGGCGAAAGTATGGGATCGAAATCTGGATCAAGAAAGTGGCCATCGTCATGGCAGAAGGTTTTCTTCGCATCCAAGAAGTTTAGGAGTATTATGCTGCTCAATTTCATCACAATTGTATATGCAAGTGACATTCCAGTTATTAAAGAAGTTGAAGCTTTCATGGATCCAGCAGCTTTCTCAGCGGTACGATTTGTCATGTCAGCCATCCCATTTCTTCCATTTGTGTTTCGATCGCGAGGTGATGTCCAGACTCGCAATTCAGGCATGGAGTTGGGGTTCTGGATCAGTCTAGGATACCTTCTTGAGGCACTTGGTTTGCTTACATCTGATGCTGGTCGTGCATCATTTATTTCTCTGTTCACAGTTATCACGGTACCTTTGCTTGATGGCATGCTTGGAGCAACCATACCTACTCGTACCTGGTTCGGAGTCCTCATGTCTGCTCTCGGGATTGCTATGCTCGAATGCAGCGGATCACCTCCGAATGTTGGAGATCTTTTGAACTTCTTGAGTGCAATATTTTTCGGAATTCACATGCTTCGAACTGAACAGATTTCCAGAAGCACAAAGAAAGAAAACTTCTTAGCAATTCTTGGATTTGAGGTTTGTGTTGTTGCTATGTTATCAACAATTTGGGTTTTCATCGGAGGGTGGTTTGATGGTACCAACTATTCCAACCAATCCCCATGGACATGGACAGAGCTGTGGGACTGGCTTGTTGTATTTCCATGGATACCAGCAATTTATACCGGTGCATTTTCAACCGGATTATGCTTATGGATTGAGATTGCAGCGATGTGTGATGTTTCAGCAACAGAAACAGCCATAATTTACGGGATGGAACCACTCTGGGGTGCTGGTTTTGCTTGGTTTCTGCTTGGTGAAAGATGGGGTATGCTTGGATGGTTAGGCGCTGCTCTTGTTCTTGGTGGAAGCTTACTGGTGCAGATTTTCGGGTCCTCATCCCCCAGAGAATTTACGGTGGCTGAAGATGGTAATGAGAAAGGTGATCTCCAACGAGTTCCAGTCGAGCATAAATTTCAAAACGGACTCTCTACTTCACCGGTTGTTGTCAGATCCGAAAAGAATGTCACAGATTTCTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

482

Amino Acids

53.06

Weight (kDa)

9.02

Isoelectric Point (pI)

44.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 143 - 266 9.4e-10 EamA-like transporter family
EamA PF00892 276 - 427 3e-11 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014745)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g17290 FvH4_5g17290 FvH4_5g17290
malus_domestica MD06G1226600.v1.1
prunus_persica Prupe.5G232800_v2.0.a1 Prupe.5G232900_v2.0.a1
pyrus_communis pycom06g20290
rosa_chinensis RchiOBHm_Chr7g0178481
rosa_laevigata RLG00000005443
rosa_multiflora Rmu_sc0010235.1_g000003
rosa_roxburghii Rroxscaffold_3G00274860
rosa_rugosa Rorug06G0417500
rosa_samantha Rh7AG016000 Rh7BG015600 Rh7CG016300 Rh7DG016000
rosa_wichuraiana Rw7G001260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 709, 998
AccB1I GGYRCC 2 cut(s) 709, 998
AccII CGCG 1 cut(s) 571
AciI CCGC 4 cut(s) 102, 121, 518, 807
AclWI GGATC 7 cut(s) 358, 370, 494, 507, 626, 817, 1407
AcoI YGGCCR 1 cut(s) 374
AcsI RAATTY 4 cut(s) 54, 864, 1310, 1373
AcuI CTGAAG 2 cut(s) 165, 1344
AfaI GTAC 5 cut(s) 237, 522, 711, 754, 1000
AfiI CCNNNNNNNGG 4 cut(s) 252, 640, 824, 1187
AgeI ACCGGT 2 cut(s) 1085, 1399
AgsI TTSAA 7 cut(s) 60, 233, 488, 838, 1098, 1379, 1441
AjnI CCWGG 1 cut(s) 755
AloI GAACNNNNNNTCC 2 cut(s) 596, 628
AluBI AGCT 8 cut(s) 38, 63, 78, 197, 491, 509, 1039, 1272
AluI AGCT 8 cut(s) 38, 63, 78, 197, 491, 509, 1039, 1272
Alw26I GTCTC 2 cut(s) 231, 320
AlwI GGATC 7 cut(s) 358, 370, 494, 507, 626, 817, 1407
Ama87I CYCGRG 1 cut(s) 782
AoxI GGCC 1 cut(s) 374
ApeKI GCWGC 6 cut(s) 63, 430, 506, 804, 1127, 1250
ApoI RAATTY 4 cut(s) 54, 864, 1310, 1373
ArsI GACNNNNNNTTYG 4 cut(s) 209, 241, 1410, 1442
AsiGI ACCGGT 2 cut(s) 1085, 1399
Asp700I GAANNNNTTC 1 cut(s) 395
Asp718I GGTACC 2 cut(s) 709, 998
AspLEI GCGC 1 cut(s) 1250
AspS9I GGNCC 1 cut(s) 1293
AsuHPI GGTGA 5 cut(s) 587, 804, 1229, 1355, 1389
AsuII TTCGAA 1 cut(s) 877
AvaI CYCGRG 1 cut(s) 782
AvaII GGWCC 1 cut(s) 1293
BalI TGGCCA 1 cut(s) 376
BamHI GGATCC 1 cut(s) 499
BanI GGYRCC 2 cut(s) 709, 998
BbvI GCAGC 6 cut(s) 50, 417, 518, 816, 1139, 1237
BceAI ACGGC 1 cut(s) 108
BciT130I CCWGG 1 cut(s) 757
BciVI GTATCC 2 cut(s) 623, 1062
BcoDI GTCTC 2 cut(s) 231, 320
BfaI CTAG 2 cut(s) 198, 626
BfoI RGCGCY 1 cut(s) 1251
BfuI GTATCC 2 cut(s) 623, 1062
BglII AGATCT 1 cut(s) 829
BisI GCNGC 7 cut(s) 64, 121, 431, 507, 805, 1128, 1251
BlsI GCNGC 7 cut(s) 65, 122, 432, 508, 806, 1129, 1252
Bme1390I CCNGG 1 cut(s) 757
Bme18I GGWCC 1 cut(s) 1293
BmeT110I CYCGRG 1 cut(s) 782
BmgT120I GGNCC 1 cut(s) 1293
BmiI GGNNCC 5 cut(s) 501, 711, 1000, 1179, 1294
BmrFI CCNGG 1 cut(s) 757
BmsI GCATC 3 cut(s) 413, 655, 686
Bpu14I TTCGAA 1 cut(s) 877
BpuEI CTTGAG 2 cut(s) 659, 865
BsaBI GATNNNNATC 1 cut(s) 355
BsaJI CCNNGG 4 cut(s) 246, 261, 1022, 1064
BsaWI WCCGGW 3 cut(s) 1085, 1100, 1399
BsaXI ACNNNNNCTCC 8 cut(s) 62, 65, 92, 95, 137, 167, 596, 626
Bsc4I CCNNNNNNNGG 4 cut(s) 252, 640, 824, 1187
Bse118I RCCGGY 2 cut(s) 1085, 1399
Bse1I ACTGG 4 cut(s) 470, 1052, 1281, 1361
Bse8I GATNNNNATC 1 cut(s) 355
BseBI CCWGG 1 cut(s) 757
BseDI CCNNGG 4 cut(s) 246, 261, 1022, 1064
BseGI GGATG 7 cut(s) 256, 328, 404, 540, 1179, 1244, 1298
BseJI GATNNNNATC 1 cut(s) 355
BseLI CCNNNNNNNGG 4 cut(s) 252, 640, 824, 1187
BseMII CTCAG 1 cut(s) 528
BseNI ACTGG 4 cut(s) 470, 1052, 1281, 1361
BseRI GAGGAG 3 cut(s) 70, 80, 88
BseXI GCAGC 6 cut(s) 50, 417, 518, 816, 1139, 1237
BsgI GTGCAG 1 cut(s) 1301
Bsh1236I CGCG 1 cut(s) 571
Bsh1285I CGRYCG 1 cut(s) 569
BshFI GGCC 1 cut(s) 376
BshNI GGYRCC 2 cut(s) 709, 998
BshTI ACCGGT 2 cut(s) 1085, 1399
BsiEI CGRYCG 1 cut(s) 569
BsiHKCI CYCGRG 1 cut(s) 782
BsiSI CCGG 3 cut(s) 1086, 1101, 1400
BslFI GGGAC 1 cut(s) 1058
BslI CCNNNNNNNGG 4 cut(s) 252, 640, 824, 1187
BsmAI GTCTC 2 cut(s) 231, 320
BsmFI GGGAC 1 cut(s) 1058
BsmI GAATGC 1 cut(s) 806
BsnI GGCC 1 cut(s) 376
BsoBI CYCGRG 1 cut(s) 782
Bsp119I TTCGAA 1 cut(s) 877
Bsp143I GATC 9 cut(s) 350, 362, 499, 566, 618, 809, 829, 1345, 1412
Bsp19I CCATGG 2 cut(s) 1022, 1064
Bsp68I TCGCGA 1 cut(s) 571
BspACI CCGC 4 cut(s) 102, 121, 518, 807
BspANI GGCC 1 cut(s) 376
BspCNI CTCAG 1 cut(s) 527
BspFNI CGCG 1 cut(s) 571
BspLI GGNNCC 5 cut(s) 501, 711, 1000, 1179, 1294
BspPI GGATC 7 cut(s) 358, 370, 494, 507, 626, 817, 1407
BspQI GCTCTTC 1 cut(s) 305
BspT104I TTCGAA 1 cut(s) 877
BspT107I GGYRCC 2 cut(s) 709, 998
BsrFI RCCGGY 2 cut(s) 1085, 1399
BsrI ACTGG 4 cut(s) 470, 1052, 1281, 1361
BssAI RCCGGY 2 cut(s) 1085, 1399
BssECI CCNNGG 4 cut(s) 246, 261, 1022, 1064
BssMI GATC 9 cut(s) 350, 362, 499, 566, 618, 809, 829, 1345, 1412
BssT1I CCWWGG 3 cut(s) 246, 1022, 1064
Bst2UI CCWGG 1 cut(s) 757
Bst4CI ACNGT 3 cut(s) 700, 709, 1318
Bst6I CTCTTC 2 cut(s) 98, 305
BstBI TTCGAA 1 cut(s) 877
BstC8I GCNNGC 2 cut(s) 7, 728
BstDEI CTNAG 3 cut(s) 330, 514, 919
BstDSI CCRYGG 2 cut(s) 1022, 1064
BstENI CCTNNNNNAGG 1 cut(s) 638
BstF5I GGATG 7 cut(s) 256, 328, 404, 540, 1179, 1244, 1298
BstFNI CGCG 1 cut(s) 571
BstH2I RGCGCY 1 cut(s) 1251
BstHHI GCGC 1 cut(s) 1250
BstKTI GATC 9 cut(s) 353, 365, 502, 569, 621, 812, 832, 1348, 1415
BstMAI GTCTC 2 cut(s) 231, 320
BstMBI GATC 9 cut(s) 350, 362, 499, 566, 618, 809, 829, 1345, 1412
BstMCI CGRYCG 1 cut(s) 569
BstMWI GCNNNNNNNGC 6 cut(s) 44, 117, 515, 597, 674, 1199
BstNI CCWGG 1 cut(s) 757
BstNSI RCATGY 3 cut(s) 9, 730, 874
BstSCI CCNGG 1 cut(s) 755
BstUI CGCG 1 cut(s) 571
BstV1I GCAGC 6 cut(s) 50, 417, 518, 816, 1139, 1237
BstX2I RGATCY 3 cut(s) 499, 829, 1412
BstXI CCANNNNNNTGG 2 cut(s) 384, 1029
BstYI RGATCY 3 cut(s) 499, 829, 1412
BsuI GTATCC 2 cut(s) 623, 1062
BsuRI GGCC 1 cut(s) 376
BtgI CCRYGG 2 cut(s) 1022, 1064
BtgZI GCGATG 2 cut(s) 291, 1145
BtsCI GGATG 7 cut(s) 256, 328, 404, 540, 1179, 1244, 1298
BtuMI TCGCGA 1 cut(s) 571
Cac8I GCNNGC 2 cut(s) 7, 728
CfoI GCGC 1 cut(s) 1250
Cfr10I RCCGGY 2 cut(s) 1085, 1399
Cfr13I GGNCC 1 cut(s) 1293
CsiI ACCWGGT 1 cut(s) 755
Csp6I GTAC 5 cut(s) 236, 521, 710, 753, 999
CspAI ACCGGT 2 cut(s) 1085, 1399
CviQI GTAC 5 cut(s) 236, 521, 710, 753, 999
DdeI CTNAG 3 cut(s) 330, 514, 919
DpnI GATC 9 cut(s) 352, 364, 501, 568, 620, 811, 831, 1347, 1414
DpnII GATC 9 cut(s) 350, 362, 499, 566, 618, 809, 829, 1345, 1412
EaeI YGGCCR 1 cut(s) 374
Eam1104I CTCTTC 2 cut(s) 98, 305
EarI CTCTTC 2 cut(s) 98, 305
EciI GGCGGA 1 cut(s) 91
Eco130I CCWWGG 3 cut(s) 246, 1022, 1064
Eco47I GGWCC 1 cut(s) 1293
Eco57I CTGAAG 2 cut(s) 165, 1344
Eco88I CYCGRG 1 cut(s) 782
EcoNI CCTNNNNNAGG 1 cut(s) 638
EcoO109I RGGNCCY 1 cut(s) 1293
EcoRI GAATTC 1 cut(s) 864
EcoRII CCWGG 1 cut(s) 755
EcoT14I CCWWGG 3 cut(s) 246, 1022, 1064
ErhI CCWWGG 3 cut(s) 246, 1022, 1064
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FaqI GGGAC 1 cut(s) 1058
Fnu4HI GCNGC 7 cut(s) 64, 121, 431, 507, 805, 1128, 1251
FokI GGATG 7 cut(s) 263, 335, 391, 527, 1186, 1251, 1285
Fsp4HI GCNGC 7 cut(s) 64, 121, 431, 507, 805, 1128, 1251
FspBI CTAG 2 cut(s) 198, 626
GlaI GCGC 1 cut(s) 1249
GluI GCNGC 7 cut(s) 64, 121, 431, 507, 805, 1128, 1251
HaeII RGCGCY 1 cut(s) 1251
HaeIII GGCC 1 cut(s) 376
HapII CCGG 3 cut(s) 1086, 1101, 1400
HhaI GCGC 1 cut(s) 1250
Hin6I GCGC 1 cut(s) 1248
HinP1I GCGC 1 cut(s) 1248
HindIII AAGCTT 2 cut(s) 489, 1270
HinfI GANTC 4 cut(s) 41, 586, 765, 1386
HpaII CCGG 3 cut(s) 1086, 1101, 1400
HphI GGTGA 5 cut(s) 587, 804, 1229, 1355, 1389
Hpy166II GTNNAC 1 cut(s) 696
Hpy188I TCNGA 8 cut(s) 295, 664, 764, 819, 863, 983, 1412, 1417
Hpy8I GTNNAC 1 cut(s) 696
HpyAV CCTTC 3 cut(s) 313, 385, 644
HpyCH4III ACNGT 3 cut(s) 700, 709, 1318
HpyCH4V TGCA 9 cut(s) 204, 271, 458, 677, 804, 851, 1091, 1127, 1282
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 117, 515, 597, 674, 1199
HpyF3I CTNAG 3 cut(s) 330, 514, 919
HspAI GCGC 1 cut(s) 1248
KpnI GGTACC 2 cut(s) 713, 1002
Kzo9I GATC 9 cut(s) 350, 362, 499, 566, 618, 809, 829, 1345, 1412
LguI GCTCTTC 1 cut(s) 305
LmnI GCTCC 3 cut(s) 83, 93, 734
Lsp1109I GCAGC 6 cut(s) 50, 417, 518, 816, 1139, 1237
LweI GCATC 3 cut(s) 413, 655, 686
MabI ACCWGGT 1 cut(s) 755
MaeI CTAG 2 cut(s) 198, 626
MaeIII GTNAC 2 cut(s) 461, 1426
MalI GATC 9 cut(s) 352, 364, 501, 568, 620, 811, 831, 1347, 1414
MboI GATC 9 cut(s) 350, 362, 499, 566, 618, 809, 829, 1345, 1412
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 3 cut(s) 499, 829, 1412
MlsI TGGCCA 1 cut(s) 376
MluNI TGGCCA 1 cut(s) 376
MlyI GAGTC 3 cut(s) 580, 774, 1380
MmeI TCCRAC 3 cut(s) 805, 1035, 1375
Mox20I TGGCCA 1 cut(s) 376
MroXI GAANNNNTTC 1 cut(s) 395
MscI TGGCCA 1 cut(s) 376
MseI TTAA 3 cut(s) 69, 306, 477
MslI CAYNNNNRTG 3 cut(s) 276, 382, 1027
Msp20I TGGCCA 1 cut(s) 376
MspA1I CMGCKG 2 cut(s) 518, 807
MspI CCGG 3 cut(s) 1086, 1101, 1400
MspR9I CCNGG 1 cut(s) 757
MunI CAATTG 1 cut(s) 447
Mva1269I GAATGC 1 cut(s) 806
MvaI CCWGG 1 cut(s) 757
MvnI CGCG 1 cut(s) 571
MwoI GCNNNNNNNGC 6 cut(s) 44, 117, 515, 597, 674, 1199
NcoI CCATGG 2 cut(s) 1022, 1064
NdeII GATC 9 cut(s) 350, 362, 499, 566, 618, 809, 829, 1345, 1412
NlaIV GGNNCC 5 cut(s) 501, 711, 1000, 1179, 1294
NmuCI GTSAC 2 cut(s) 461, 1426
NruI TCGCGA 1 cut(s) 571
NspI RCATGY 3 cut(s) 9, 730, 874
NspV TTCGAA 1 cut(s) 877
PaeI GCATGC 2 cut(s) 9, 730
PciSI GCTCTTC 1 cut(s) 305
PctI GAATGC 1 cut(s) 806
PdmI GAANNNNTTC 1 cut(s) 395
PfeI GAWTC 1 cut(s) 41
PinAI ACCGGT 2 cut(s) 1085, 1399
PkrI GCNGC 7 cut(s) 65, 122, 432, 508, 806, 1129, 1252
Ple19I CGATCG 1 cut(s) 569
PleI GAGTC 3 cut(s) 580, 773, 1380
PpsI GAGTC 3 cut(s) 580, 773, 1380
PpuMI RGGWCCY 1 cut(s) 1293
Psp5II RGGWCCY 1 cut(s) 1293
Psp6I CCWGG 1 cut(s) 755
PspGI CCWGG 1 cut(s) 755
PspN4I GGNNCC 5 cut(s) 501, 711, 1000, 1179, 1294
PspPI GGNCC 1 cut(s) 1293
PspPPI RGGWCCY 1 cut(s) 1293
PsuI RGATCY 3 cut(s) 499, 829, 1412
PvuI CGATCG 1 cut(s) 569
RruI TCGCGA 1 cut(s) 571
RsaI GTAC 5 cut(s) 237, 522, 711, 754, 1000
RsaNI GTAC 5 cut(s) 236, 521, 710, 753, 999
RseI CAYNNNNRTG 3 cut(s) 276, 382, 1027
SapI GCTCTTC 1 cut(s) 305
SaqAI TTAA 3 cut(s) 69, 306, 477
SatI GCNGC 7 cut(s) 64, 121, 431, 507, 805, 1128, 1251
Sau3AI GATC 9 cut(s) 350, 362, 499, 566, 618, 809, 829, 1345, 1412
Sau96I GGNCC 1 cut(s) 1293
SchI GAGTC 3 cut(s) 580, 774, 1380
ScrFI CCNGG 1 cut(s) 757
SexAI ACCWGGT 1 cut(s) 755
SfaNI GCATC 3 cut(s) 413, 655, 686
SfuI TTCGAA 1 cut(s) 877
SinI GGWCC 1 cut(s) 1293
SmiMI CAYNNNNRTG 3 cut(s) 276, 382, 1027
SmlI CTYRAG 2 cut(s) 638, 844
SmoI CTYRAG 2 cut(s) 638, 844
SphI GCATGC 2 cut(s) 9, 730
SsiI CCGC 4 cut(s) 102, 121, 518, 807
SspI AATATT 1 cut(s) 855
SspMI CTAG 2 cut(s) 198, 626
StyD4I CCNGG 1 cut(s) 755
StyI CCWWGG 3 cut(s) 246, 1022, 1064
TaaI ACNGT 3 cut(s) 700, 709, 1318
TaqI TCGA 5 cut(s) 353, 565, 798, 877, 1365
TatI WGTACW 1 cut(s) 235
TauI GCSGC 1 cut(s) 123
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 3 cut(s) 69, 306, 477
Tru9I TTAA 3 cut(s) 69, 306, 477
TseFI GTSAC 2 cut(s) 461, 1426
TseI GCWGC 6 cut(s) 63, 430, 506, 804, 1127, 1250
Tsp45I GTSAC 2 cut(s) 461, 1426
TspDTI ATGAA 4 cut(s) 150, 430, 484, 967
TspGWI ACGGA 1 cut(s) 1398
VpaK11BI GGWCC 1 cut(s) 1293
XagI CCTNNNNNAGG 1 cut(s) 638
XapI RAATTY 4 cut(s) 54, 864, 1310, 1373
XceI RCATGY 3 cut(s) 9, 730, 874
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 2 cut(s) 198, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.