pycom07g01340

Coiled-coil region of Oberon

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008405
Physical Location & Seq
Reverse (-)
1006058 .. 1009036
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g01340.1

Sequence Viewer

Length: 2979 bp
ATGAAGAGGCTGAGATCCAGCGACGATCTCGATTCCTTCGGGAAGGATCCGAATCCGAATCCTAACCCAAACTCCAGCTCCAACCCCAGCCGGACCTCCTCCAACTCGCACCGGAGCTTCTACTATAAGCCAGATACTGTGCGGAAGGGTTTGCTCTCTTCGGCCTCCTCGGCTTCTTCTCTGGTGCCGGCTCGCTCCTACGACGATCGGGAGCAGGCCGGTTCTGGCGGAGGGTCCAGAACTGTCCGGAAGAGGCCGGGGCAAGAGTTCGACGGTTTCGACCGGAGGAAGGGGACCGATCGATACAATAGAGATGGAGGAGGGTACGATCGTAATTTGATGCACCGGTCCGAGAGCTTCTCCGCGTCGAGGAGGTCGCCGGCGGATTTTCCGAAAGGGTTTCGATCGGAGCGGGACAGGTCGAGGCGCGAAGGCAGCGGGAGCGGAGCGTTGTCGTGGCGGAGGTTTGGGAAGGAGTTCGAGGAGAGAGGAGGAGGGAAGGGGTTGAGGGACGTGAGGTCGCCGACGTGGTCGAATTTGAGGGATTCAGGGAGCGAGCAGTCTAGGGTTAGGTCTCCGGTGAGGAGGTTGAGGGATGGGAAGGAGTCGAAATCGGAGTCGAAGTCCAAGTCGCCCACTTGGTCTAAGGACTCCGTGGGGAGTGAGCAGTCGAAGAGTGTTGAGGTGAGGAAGAGGGAGGTGGAGGCGGAGGAGGTTCAGGGTGAGCAGCCAAAGAATGTTGAGTTTAGGAAGAAGGAGACGGAGGAGGTTCCGGTTGAGCAGCCAAAGAGTGTTGAGGTTAGGAAGAGGGAGACGGAGGAGGTTCAGGCTGATAAGGGGAGTAGGACTAGTAGTGAAATAGAGGAGGGTGAGCTTGCGCCGGAAGCTGGAGCTGGTGTTGGAGGTGGAGGTGAAGGTGAGCCCCAATTGGGTCCTGAAGGTGGAGCTGAAACAGGAGAAACACGGATTCGAAGCGAGACTTCAGATAGGGTTGAGGAGAAAGGTGAGTCTCTGGATAAACAAGAGGCTAGGGAGGAGAAGGGTGAGTCCTCGGATAAACAAGAGGTGAAAGATGTGAGTAAAGAGAGTGTGTGTGAAAGGAAGGATGAGGAGGAGAAGAAAGATGATGACTTGCCAAATGGTGATAACGATATGATTGATAAAAGCGGGAATGTGGAAGCTTGTGAAGATGAGGATGATGGAAAAGGGAGTGTGAGGGAAGGTGATGAAGAAGTGGTCGAGATTCCGATGGAATTGGAAGATGAACCAAAGCAAGATAAGGGAATTGACCTTGAATTGAAGGCGGAGGATGATGACGATGAAATGATAGAGTCAGACAAGGTAGTAACAGAGGATGAGGAGGATAATGAAGTGGTTAAGCTAGATACGCTGAATATAAGGCAGAATTTTAAGGATAAAGGTAAAAGCGTCGCTGTGACACCAACTGATATGGTAGATTCAGCAGAAGATGGTCAGTGGACTGCTAGAGAATCCAGAGAGCTGTTAACTGGCATGGAAAATGATATAGAAGGACCCAGCACTAGGGGTTTCGAGTTGTTTTCAAGTTCTCCTGTTAGGAGACAGGAGAAAGCTGATCAATCTGGTTCCATTATGAAGGATGAAAAGCTGGCACTTGAGCCGCTTGATCTTTCTCTTAGCTTGCCTAATGTTTTGTTACCCGTTGGTGGTGCAGCTCCTGGTTCTCCTGACCAAGCAATGAGTGTTCAGTCTCTAAGTAATACTCTTCGTACTAACTCTGATGGATTTACTCAATCAGTATCCTTTTCAGGCTCTCAGTCGTTTTATCACAACCCTAGCTGTTCGCTAACCACCCAGAATTCGATGGACTTTGAACAGTCGGTTAAAAGTCGTCCGCTTTTCCAGGGAATCGATTGGCAGGCACTGGCTCAGAGTGAGGCTAAGGGTAAAGAAGTTCCTTCGCAGGCACTTGTTCAGAATGATGCTAAGAGCAAAGAAATCCCTTTGTATCAAAGAATCTTGATGAATGGAAACGGGTCTCATCAACAACAGTCTCAAGCATCGCAAGGCATTCCAAATGGTCAATCGGTCCAAGGGCAACAACATCGAAGGCATACTGAAGGAAGCTCTGAAGTGGAGAATGGAATGGAAAGGCAGCTGAGCTTCAATAAACAGTTGTCAGGAGGACAGACAAGGAACCATGAGGATGTTAGATCACCTTCGAATAGCGTTGGATCTCATGAAATGGGATCGAACTATAGTTTTGACAGAAAACGACTATTGAGAGAGAAAAATAGTGGTAGTTTATATAGGACTAGCAGTCAGAAGGAACACAAGAAGTTTCTCATTGGTGGAGCTGACTTTGTGGAGACAATCGTTGCCAGGATAGTCTCCGATCCCATACATGTAATGGCTAGAAAGTTTCATGAGATGACAGGACAATCGGCTTCATGTGTGAAGGAGACCATTCGCGATATGATGTTAAATATGGATAAGCGCATGCAACTGTTTGCATTTCAGAAGGCACTGCAGAGCAGGTCTGATATAACCATGGAGATGCTACTAAAAGCTCATCGTGCGCAACTGGAAATCTTGGTTGCTCTGAAGACTGGTCTACCGAATTATCTCCTGCAAGAGAATGGTGCATCATCTTCTGATTTGGCTCAAATTTTTCTGAACTCAAGATGCAGAAATCCTTCTTGTTGGAGTCCTGTGCCTGTGGATGAATGTGATTGCAAGGTTTGTTCGCCAAAGACTGGTTTTTGCAGTGCGTGTATGTGTCTTGTGTGCTCAAAGTTTGACATGGCCTCAAATACATGTAGTTGGATTGGGTGTGATGTTTGTCTCCATTGGTGCCACGCGGATTGCGCATTGCGGGAATCTTATATTAGAAATGGACGCAGTGCTACTGGATCTCAAGGGACGACTGAGATGCAGTTTCACTGTGTTGCCTGTCCACATTCTCGATCGATCATCCTTCTGAGATATTTGGGTTCGTGA

Protein Analysis

993

Amino Acids

109.96

Weight (kDa)

5.31

Isoelectric Point (pI)

63.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD_Oberon PF07227 889 - 985 5.2e-28 PHD - plant homeodomain finger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 2559, 2848
Acc36I ACCTGC 1 cut(s) 2505
AccB1I GGYRCC 2 cut(s) 184, 2832
AccB7I CCANNNNNTGG 1 cut(s) 2735
AccBSI CCGCTC 2 cut(s) 412, 444
AccI GTMKAC 1 cut(s) 2593
AccII CGCG 4 cut(s) 365, 429, 2451, 2840
AccIII TCCGGA 1 cut(s) 246
AclWI GGATC 7 cut(s) 9, 41, 54, 2221, 2236, 2369, 2899
AcsI RAATTY 4 cut(s) 535, 1405, 1837, 2646
AcuI CTGAAG 5 cut(s) 957, 966, 2118, 2130, 2603
AfaI GTAC 2 cut(s) 326, 1750
AfiI CCNNNNNNNGG 8 cut(s) 289, 369, 887, 929, 941, 1542, 1685, 2735
AflIII ACRYGT 2 cut(s) 2383, 2795
AgeI ACCGGT 1 cut(s) 345
AgsI TTSAA 5 cut(s) 1295, 1300, 1563, 1853, 2146
AhdI GACNNNNNGTC 2 cut(s) 517, 2298
AhlI ACTAGT 1 cut(s) 848
AjiI CACGTC 2 cut(s) 514, 528
AjnI CCWGG 3 cut(s) 1696, 1881, 2360
Alw21I GWGCWC 1 cut(s) 2771
AlwI GGATC 7 cut(s) 9, 41, 54, 2221, 2236, 2369, 2899
AlwNI CAGNNNCTG 3 cut(s) 137, 1697, 1903
Aor13HI TCCGGA 1 cut(s) 246
AoxI GGCC 4 cut(s) 162, 216, 254, 2784
ApeKI GCWGC 5 cut(s) 435, 727, 781, 1691, 2134
ApoI RAATTY 4 cut(s) 535, 1405, 1837, 2646
ArsI GACNNNNNNTTYG 4 cut(s) 602, 614, 634, 646
AsiGI ACCGGT 1 cut(s) 345
Asp700I GAANNNNTTC 2 cut(s) 476, 2674
AspLEI GCGC 5 cut(s) 429, 880, 2478, 2560, 2849
AspS9I GGNCC 7 cut(s) 93, 234, 294, 348, 932, 1532, 2068
AsuC2I CCSGG 1 cut(s) 258
AsuII TTCGAA 2 cut(s) 970, 2201
AvaII GGWCC 7 cut(s) 93, 234, 294, 348, 932, 1532, 2068
BaeI ACNNNNGTAYC 2 cut(s) 316, 349
BamHI GGATCC 1 cut(s) 46
BanI GGYRCC 2 cut(s) 184, 2832
BanII GRGCYC 1 cut(s) 924
BbsI GAAGAC 1 cut(s) 2591
Bbv12I GWGCWC 1 cut(s) 2771
BbvI GCAGC 5 cut(s) 447, 739, 793, 1703, 2146
BccI CCATC 7 cut(s) 308, 590, 1193, 1243, 1463, 1754, 1837
BcgI CGANNNNNNTGC 4 cut(s) 2066, 2100, 2893, 2927
BciT130I CCWGG 3 cut(s) 1698, 1883, 2362
BciVI GTATCC 1 cut(s) 1789
BclI TGATCA 1 cut(s) 1594
BcnI CCSGG 1 cut(s) 258
BcuI ACTAGT 1 cut(s) 848
BfaI CTAG 9 cut(s) 564, 849, 1029, 1382, 1485, 1542, 1815, 2295, 2394
BfmI CTRYAG 2 cut(s) 2236, 2507
BfuAI ACCTGC 1 cut(s) 2505
BfuI GTATCC 1 cut(s) 1789
BglI GCCNNNNNGGC 1 cut(s) 170
BisI GCNGC 6 cut(s) 436, 728, 782, 1640, 1692, 2135
BlpI GCTNAGC 1 cut(s) 2138
BlsI GCNGC 6 cut(s) 437, 729, 783, 1641, 1693, 2136
Bme1390I CCNGG 4 cut(s) 258, 1698, 1883, 2362
Bme18I GGWCC 7 cut(s) 93, 234, 294, 348, 932, 1532, 2068
BmeRI GACNNNNNGTC 2 cut(s) 517, 2298
BmgBI CACGTC 2 cut(s) 514, 528
BmgT120I GGNCC 7 cut(s) 93, 234, 294, 348, 932, 1532, 2068
BmrFI CCNGG 4 cut(s) 258, 1698, 1883, 2362
BmsI GCATC 7 cut(s) 330, 1951, 2048, 2526, 2633, 2654, 2901
BpiI GAAGAC 1 cut(s) 2591
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpmI CTGGAG 2 cut(s) 58, 909
Bpu10I CCTNAGC 1 cut(s) 1920
Bpu1102I GCTNAGC 1 cut(s) 2138
Bpu14I TTCGAA 2 cut(s) 970, 2201
BpuEI CTTGAG 4 cut(s) 1655, 2019, 2644, 2880
BpuMI CCSGG 1 cut(s) 258
Bsa29I ATCGAT 3 cut(s) 301, 1890, 2948
BsaBI GATNNNNATC 1 cut(s) 51
BsaI GGTCTC 3 cut(s) 579, 2022, 2435
BsaJI CCNNGG 7 cut(s) 168, 257, 654, 1050, 1882, 2071, 2529
BsaWI WCCGGW 6 cut(s) 111, 246, 282, 345, 577, 772
BsaXI ACNNNNNCTCC 8 cut(s) 56, 62, 86, 92, 608, 638, 882, 912
Bsc4I CCNNNNNNNGG 8 cut(s) 289, 369, 887, 929, 941, 1542, 1685, 2735
Bse118I RCCGGY 4 cut(s) 187, 218, 345, 379
Bse1I ACTGG 6 cut(s) 1513, 1908, 2568, 2593, 2740, 2893
Bse3DI GCAATG 2 cut(s) 1722, 2849
Bse8I GATNNNNATC 1 cut(s) 51
BseAI TCCGGA 1 cut(s) 246
BseBI CCWGG 3 cut(s) 1698, 1883, 2362
BseCI ATCGAT 3 cut(s) 301, 1890, 2948
BseDI CCNNGG 7 cut(s) 168, 257, 654, 1050, 1882, 2071, 2529
BseGI GGATG 9 cut(s) 601, 1111, 1201, 1315, 1360, 1624, 2191, 2707, 2952
BseJI GATNNNNATC 1 cut(s) 51
BseLI CCNNNNNNNGG 8 cut(s) 289, 369, 887, 929, 941, 1542, 1685, 2735
BseMI GCAATG 2 cut(s) 1722, 2849
BseMII CTCAG 5 cut(s) 1808, 1922, 2129, 2898, 2951
BseNI ACTGG 6 cut(s) 1513, 1908, 2568, 2593, 2740, 2893
BseXI GCAGC 5 cut(s) 447, 739, 793, 1703, 2146
BseYI CCCAGC 2 cut(s) 86, 1535
BsgI GTGCAG 1 cut(s) 1710
Bsh1236I CGCG 4 cut(s) 365, 429, 2451, 2840
Bsh1285I CGRYCG 6 cut(s) 208, 283, 301, 331, 407, 2948
BshFI GGCC 4 cut(s) 164, 218, 256, 2786
BshNI GGYRCC 2 cut(s) 184, 2832
BshTI ACCGGT 1 cut(s) 345
BshVI ATCGAT 3 cut(s) 301, 1890, 2948
BsiEI CGRYCG 6 cut(s) 208, 283, 301, 331, 407, 2948
BsiHKAI GWGCWC 1 cut(s) 2771
BslFI GGGAC 4 cut(s) 307, 428, 524, 2914
BslI CCNNNNNNNGG 8 cut(s) 289, 369, 887, 929, 941, 1542, 1685, 2735
BsmBI CGTCTC 2 cut(s) 752, 806
BsmFI GGGAC 4 cut(s) 307, 428, 524, 2914
BsmI GAATGC 1 cut(s) 2049
BsnI GGCC 4 cut(s) 164, 218, 256, 2786
Bso31I GGTCTC 3 cut(s) 579, 2022, 2435
Bsp119I TTCGAA 2 cut(s) 970, 2201
Bsp1286I GDGCHC 2 cut(s) 924, 2771
Bsp13I TCCGGA 1 cut(s) 246
Bsp1720I GCTNAGC 1 cut(s) 2138
Bsp19I CCATGG 1 cut(s) 2529
Bsp68I TCGCGA 1 cut(s) 2451
BspANI GGCC 4 cut(s) 164, 218, 256, 2786
BspCNI CTCAG 5 cut(s) 1807, 1921, 2130, 2899, 2952
BspDI ATCGAT 3 cut(s) 301, 1890, 2948
BspEI TCCGGA 1 cut(s) 246
BspFNI CGCG 4 cut(s) 365, 429, 2451, 2840
BspHI TCATGA 2 cut(s) 2218, 2404
BspMAI CTGCAG 1 cut(s) 2511
BspMI ACCTGC 1 cut(s) 2505
BspPI GGATC 7 cut(s) 9, 41, 54, 2221, 2236, 2369, 2899
BspT104I TTCGAA 2 cut(s) 970, 2201
BspT107I GGYRCC 2 cut(s) 184, 2832
BspTNI GGTCTC 3 cut(s) 579, 2022, 2435
BsrBI CCGCTC 2 cut(s) 412, 444
BsrDI GCAATG 2 cut(s) 1722, 2849
BsrFI RCCGGY 4 cut(s) 187, 218, 345, 379
BsrI ACTGG 6 cut(s) 1513, 1908, 2568, 2593, 2740, 2893
BssAI RCCGGY 4 cut(s) 187, 218, 345, 379
BssECI CCNNGG 7 cut(s) 168, 257, 654, 1050, 1882, 2071, 2529
BssT1I CCWWGG 2 cut(s) 2071, 2529
Bst2UI CCWGG 3 cut(s) 1698, 1883, 2362
Bst4CI ACNGT 8 cut(s) 139, 244, 275, 1857, 2031, 2154, 2487, 2924
Bst6I CTCTTC 6 cut(s) 163, 245, 668, 686, 800, 1749
BstBI TTCGAA 2 cut(s) 970, 2201
BstDSI CCRYGG 2 cut(s) 654, 2529
BstF5I GGATG 9 cut(s) 601, 1111, 1201, 1315, 1360, 1624, 2191, 2707, 2952
BstFNI CGCG 4 cut(s) 365, 429, 2451, 2840
BstHHI GCGC 5 cut(s) 429, 880, 2478, 2560, 2849
BstMCI CGRYCG 6 cut(s) 208, 283, 301, 331, 407, 2948
BstMWI GCNNNNNNNGC 7 cut(s) 170, 435, 441, 884, 1387, 2555, 2846
BstNI CCWGG 3 cut(s) 1698, 1883, 2362
BstNSI RCATGY 3 cut(s) 2387, 2482, 2799
BstSCI CCNGG 4 cut(s) 256, 1696, 1881, 2360
BstSFI CTRYAG 2 cut(s) 2236, 2507
BstUI CGCG 4 cut(s) 365, 429, 2451, 2840
BstV1I GCAGC 5 cut(s) 447, 739, 793, 1703, 2146
BstV2I GAAGAC 1 cut(s) 2591
BstX2I RGATCY 4 cut(s) 14, 46, 2213, 2891
BstYI RGATCY 4 cut(s) 14, 46, 2213, 2891
Bsu15I ATCGAT 3 cut(s) 301, 1890, 2948
BsuI GTATCC 1 cut(s) 1789
BsuRI GGCC 4 cut(s) 164, 218, 256, 2786
BsuTUI ATCGAT 3 cut(s) 301, 1890, 2948
BtgI CCRYGG 2 cut(s) 654, 2529
BtgZI GCGATG 1 cut(s) 2025
BtrI CACGTC 2 cut(s) 514, 528
BtsCI GGATG 9 cut(s) 601, 1111, 1201, 1315, 1360, 1624, 2191, 2707, 2952
BtsI GCAGTG 3 cut(s) 2504, 2752, 2887
BtsIMutI CAGTG 6 cut(s) 1481, 1901, 2504, 2752, 2887, 2920
BtuMI TCGCGA 1 cut(s) 2451
BveI ACCTGC 1 cut(s) 2505
CaiI CAGNNNCTG 3 cut(s) 137, 1697, 1903
CciI TCATGA 2 cut(s) 2218, 2404
CfoI GCGC 5 cut(s) 429, 880, 2478, 2560, 2849
Cfr10I RCCGGY 4 cut(s) 187, 218, 345, 379
Cfr13I GGNCC 7 cut(s) 93, 234, 294, 348, 932, 1532, 2068
ClaI ATCGAT 3 cut(s) 301, 1890, 2948
CpoI CGGWCCG 1 cut(s) 348
CseI GACGC 3 cut(s) 354, 1417, 2886
Csp6I GTAC 2 cut(s) 325, 1749
CspAI ACCGGT 1 cut(s) 345
CspCI CAANNNNNGTGG 2 cut(s) 2825, 2860
CspI CGGWCCG 1 cut(s) 348
CviQI GTAC 2 cut(s) 325, 1749
DriI GACNNNNNGTC 2 cut(s) 517, 2298
Eam1104I CTCTTC 6 cut(s) 163, 245, 668, 686, 800, 1749
Eam1105I GACNNNNNGTC 2 cut(s) 517, 2298
EarI CTCTTC 6 cut(s) 163, 245, 668, 686, 800, 1749
EciI GGCGGA 5 cut(s) 243, 398, 475, 722, 1319
Eco130I CCWWGG 2 cut(s) 2071, 2529
Eco24I GRGCYC 1 cut(s) 924
Eco31I GGTCTC 3 cut(s) 579, 2022, 2435
Eco47I GGWCC 7 cut(s) 93, 234, 294, 348, 932, 1532, 2068
Eco57I CTGAAG 5 cut(s) 957, 966, 2118, 2130, 2603
EcoO109I RGGNCCY 2 cut(s) 932, 1532
EcoRI GAATTC 1 cut(s) 1837
EcoRII CCWGG 3 cut(s) 1696, 1881, 2360
EcoT14I CCWWGG 2 cut(s) 2071, 2529
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 2 cut(s) 2071, 2529
Esp3I CGTCTC 2 cut(s) 752, 806
FalI AAGNNNNNCTT 2 cut(s) 964, 996
FaqI GGGAC 4 cut(s) 307, 428, 524, 2914
FauI CCCGC 4 cut(s) 405, 431, 1160, 2847
FbaI TGATCA 1 cut(s) 1594
FblI GTMKAC 1 cut(s) 2593
Fnu4HI GCNGC 6 cut(s) 436, 728, 782, 1640, 1692, 2135
FokI GGATG 9 cut(s) 608, 1118, 1208, 1322, 1367, 1631, 2198, 2714, 2939
FriOI GRGCYC 1 cut(s) 924
Fsp4HI GCNGC 6 cut(s) 436, 728, 782, 1640, 1692, 2135
FspBI CTAG 9 cut(s) 564, 849, 1029, 1382, 1485, 1542, 1815, 2295, 2394
FspI TGCGCA 2 cut(s) 2559, 2848
GlaI GCGC 5 cut(s) 428, 879, 2477, 2559, 2848
GluI GCNGC 6 cut(s) 436, 728, 782, 1640, 1692, 2135
GsaI CCCAGC 2 cut(s) 90, 1539
GsuI CTGGAG 2 cut(s) 58, 909
HaeIII GGCC 4 cut(s) 164, 218, 256, 2786
HgaI GACGC 3 cut(s) 354, 1417, 2886
HhaI GCGC 5 cut(s) 429, 880, 2478, 2560, 2849
Hin6I GCGC 5 cut(s) 427, 878, 2476, 2558, 2847
HinP1I GCGC 5 cut(s) 427, 878, 2476, 2558, 2847
HincII GTYRAC 1 cut(s) 1506
HindII GTYRAC 1 cut(s) 1506
HindIII AAGCTT 1 cut(s) 1179
HpaI GTTAAC 1 cut(s) 1506
Hpy166II GTNNAC 4 cut(s) 1479, 1506, 2594, 2936
Hpy8I GTNNAC 4 cut(s) 1479, 1506, 2594, 2936
Hpy99I CGWCG 6 cut(s) 26, 206, 275, 370, 529, 1433
HpyCH4III ACNGT 8 cut(s) 139, 244, 275, 1857, 2031, 2154, 2487, 2924
HpyCH4IV ACGT 2 cut(s) 513, 527
HpyF10VI GCNNNNNNNGC 7 cut(s) 170, 435, 441, 884, 1387, 2555, 2846
HpySE526I ACGT 2 cut(s) 513, 527
HspAI GCGC 5 cut(s) 427, 878, 2476, 2558, 2847
Kpn2I TCCGGA 1 cut(s) 246
KroI GCCGGC 2 cut(s) 187, 379
KroNI GCCGGC 2 cut(s) 189, 381
Ksp22I TGATCA 1 cut(s) 1594
KspAI GTTAAC 1 cut(s) 1506
Lsp1109I GCAGC 5 cut(s) 447, 739, 793, 1703, 2146
LweI GCATC 7 cut(s) 330, 1951, 2048, 2526, 2633, 2654, 2901
MaeI CTAG 9 cut(s) 564, 849, 1029, 1382, 1485, 1542, 1815, 2295, 2394
MaeII ACGT 2 cut(s) 513, 527
MaeIII GTNAC 3 cut(s) 1345, 1435, 1674
MbiI CCGCTC 2 cut(s) 412, 444
MfeI CAATTG 1 cut(s) 926
MflI RGATCY 4 cut(s) 14, 46, 2213, 2891
MhlI GDGCHC 2 cut(s) 924, 2771
MlyI GAGTC 7 cut(s) 614, 626, 644, 1016, 1055, 1340, 2695
MmeI TCCRAC 6 cut(s) 105, 126, 880, 2191, 2663, 2783
MreI CGCCGGCG 1 cut(s) 379
MroI TCCGGA 1 cut(s) 246
MroNI GCCGGC 2 cut(s) 187, 379
MroXI GAANNNNTTC 2 cut(s) 476, 2674
MseI TTAA 5 cut(s) 1377, 1410, 1505, 1863, 2462
MslI CAYNNNNRTG 2 cut(s) 2184, 2534
MspA1I CMGCKG 2 cut(s) 438, 2137
MspR9I CCNGG 4 cut(s) 258, 1698, 1883, 2362
MunI CAATTG 1 cut(s) 926
Mva1269I GAATGC 1 cut(s) 2049
MvaI CCWGG 3 cut(s) 1698, 1883, 2362
MvnI CGCG 4 cut(s) 365, 429, 2451, 2840
MwoI GCNNNNNNNGC 7 cut(s) 170, 435, 441, 884, 1387, 2555, 2846
NaeI GCCGGC 2 cut(s) 189, 381
NciI CCSGG 1 cut(s) 258
NcoI CCATGG 1 cut(s) 2529
NgoMIV GCCGGC 2 cut(s) 187, 379
NmeAIII GCCGAG 1 cut(s) 149
NmuCI GTSAC 1 cut(s) 1435
NruI TCGCGA 1 cut(s) 2451
NsbI TGCGCA 2 cut(s) 2559, 2848
NspI RCATGY 3 cut(s) 2387, 2482, 2799
NspV TTCGAA 2 cut(s) 970, 2201
PaeI GCATGC 1 cut(s) 2482
PagI TCATGA 2 cut(s) 2218, 2404
PciI ACATGT 2 cut(s) 2383, 2795
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 2049
PdiI GCCGGC 2 cut(s) 189, 381
PdmI GAANNNNTTC 2 cut(s) 476, 2674
PflFI GACNNNGTC 1 cut(s) 529
PflMI CCANNNNNTGG 1 cut(s) 2735
PinAI ACCGGT 1 cut(s) 345
PkrI GCNGC 6 cut(s) 437, 729, 783, 1641, 1693, 2136
Ple19I CGATCG 5 cut(s) 208, 301, 331, 407, 2948
PleI GAGTC 7 cut(s) 613, 625, 644, 1015, 1054, 1339, 2694
PpsI GAGTC 7 cut(s) 613, 625, 644, 1015, 1054, 1339, 2694
PpuMI RGGWCCY 2 cut(s) 932, 1532
PscI ACATGT 2 cut(s) 2383, 2795
Psp5II RGGWCCY 2 cut(s) 932, 1532
Psp6I CCWGG 3 cut(s) 1696, 1881, 2360
PspFI CCCAGC 2 cut(s) 86, 1535
PspGI CCWGG 3 cut(s) 1696, 1881, 2360
PspPI GGNCC 7 cut(s) 93, 234, 294, 348, 932, 1532, 2068
PspPPI RGGWCCY 2 cut(s) 932, 1532
PstI CTGCAG 1 cut(s) 2511
PstNI CAGNNNCTG 3 cut(s) 137, 1697, 1903
PsuI RGATCY 4 cut(s) 14, 46, 2213, 2891
PsyI GACNNNGTC 1 cut(s) 529
PvuI CGATCG 5 cut(s) 208, 301, 331, 407, 2948
PvuII CAGCTG 1 cut(s) 2137
RruI TCGCGA 1 cut(s) 2451
RsaI GTAC 2 cut(s) 326, 1750
RsaNI GTAC 2 cut(s) 325, 1749
RseI CAYNNNNRTG 2 cut(s) 2184, 2534
Rsr2I CGGWCCG 1 cut(s) 348
RsrII CGGWCCG 1 cut(s) 348
SaqAI TTAA 5 cut(s) 1377, 1410, 1505, 1863, 2462
SatI GCNGC 6 cut(s) 436, 728, 782, 1640, 1692, 2135
Sau96I GGNCC 7 cut(s) 93, 234, 294, 348, 932, 1532, 2068
SchI GAGTC 7 cut(s) 614, 626, 644, 1016, 1055, 1340, 2695
ScrFI CCNGG 4 cut(s) 258, 1698, 1883, 2362
SduI GDGCHC 2 cut(s) 924, 2771
SfaNI GCATC 7 cut(s) 330, 1951, 2048, 2526, 2633, 2654, 2901
SfcI CTRYAG 2 cut(s) 2236, 2507
SfuI TTCGAA 2 cut(s) 970, 2201
SgrAI CRCCGGYG 1 cut(s) 379
SinI GGWCC 7 cut(s) 93, 234, 294, 348, 932, 1532, 2068
SmiMI CAYNNNNRTG 2 cut(s) 2184, 2534
SmlI CTYRAG 4 cut(s) 1634, 2034, 2659, 2895
SmoI CTYRAG 4 cut(s) 1634, 2034, 2659, 2895
SpeI ACTAGT 1 cut(s) 848
SphI GCATGC 1 cut(s) 2482
SspMI CTAG 9 cut(s) 564, 849, 1029, 1382, 1485, 1542, 1815, 2295, 2394
StyD4I CCNGG 4 cut(s) 256, 1696, 1881, 2360
StyI CCWWGG 2 cut(s) 2071, 2529
TaaI ACNGT 8 cut(s) 139, 244, 275, 1857, 2031, 2154, 2487, 2924
TaiI ACGT 2 cut(s) 516, 530
TaqII GACCGA 2 cut(s) 311, 2056
TauI GCSGC 1 cut(s) 1642
Tru1I TTAA 5 cut(s) 1377, 1410, 1505, 1863, 2462
Tru9I TTAA 5 cut(s) 1377, 1410, 1505, 1863, 2462
TscAI CASTG 6 cut(s) 1481, 1908, 2511, 2752, 2887, 2927
TseFI GTSAC 1 cut(s) 1435
TseI GCWGC 5 cut(s) 435, 727, 781, 1691, 2134
Tsp45I GTSAC 1 cut(s) 1435
TspGWI ACGGA 4 cut(s) 643, 776, 830, 979
TspRI CASTG 6 cut(s) 1481, 1908, 2511, 2752, 2887, 2927
Tth111I GACNNNGTC 1 cut(s) 529
Van91I CCANNNNNTGG 1 cut(s) 2735
VpaK11BI GGWCC 7 cut(s) 93, 234, 294, 348, 932, 1532, 2068
XapI RAATTY 4 cut(s) 535, 1405, 1837, 2646
XceI RCATGY 3 cut(s) 2387, 2482, 2799
XcmI CCANNNNNNNNNTGG 1 cut(s) 2386
XmiI GTMKAC 1 cut(s) 2593
XmnI GAANNNNTTC 2 cut(s) 476, 2674
XspI CTAG 9 cut(s) 564, 849, 1029, 1382, 1485, 1542, 1815, 2295, 2394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.