Rorug01G0013900

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001532
Physical Location & Seq
Forward (+)
2422682 .. 2427019
4338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0013900.1

Sequence Viewer

Length: 1785 bp
ATGGGAGAGCAGCAAGATATGCATGAACAAGTGAGCTTTTGGAAGATGCCTGTTGATGTTTTCTTCAGGGATGCAAGGAATGTTTTCAAATGGGATGTGCTTGGTAAGGAGATACTGCAGATTGCAGTCCCTGCTGCTCTGGCTGTAGCTGCTGATCCTATCGCTTCCTTAATCGACACAGCTTTCATTGGTCATATAGGTCCAGTGGAACTAGCGGCAGCAGGAGTATCTATTGCCTTGTTCAATCAAGCTTCAAGGATTACTATATTCCCACTTGTCAGTATCACCACCTCCTTTGTTGCCGAGGAAGATACTGTAGCAAAAATGAAAATAAAAGCAGGAAAAGGCCAGAAATTGGAGAAGGGTTCAGACATGCTTGATGACCTGGAAAAAGGGGTTGCTAAACCAAAACCTGAGACGAAAGAGCCTGCACTAGATGATGAAAATGGTGAATCCTCAGAAATGGGCAAGCATCTGGGAAATGATGAAGACAAGGAAAATGGTGAGAACTCAGACAAGATACTAGATGAAGGCAGTGAAGTAAAGAATTCCATATCGGAAAATGCTAAGCTGGAGAATGGAGAGAAAGGTGTTCCTAAAAACAGTGAACCACAAGAAAATGGTTCCAAGACAAATATTGAAACCGGTCCATCTGTTGTTACGAGCAAAAATAAGAAGCCAAAGATGAAGAACAAAAAGCTTAATATTGCTTCCGCATCAACAGCACTCATCTTCGGTGCAATACTTGGCCTCCTGCAAGCTATATTTCTCATGTTAGGATCCAAAGTTCTTCTTGGTGTGATGGGTGTGAAACATGACTCCCCTATGCTAAAACCAGCAATGAAGTACTTGAGAGTAAGGTCGATCGGTGCACCTGCTGTTCTTCTTACATTGGCCATGCAAGGAATCTTCCGAGGGTTTAGGGATACTAAAACTCCTTTATATGTCATCGTTGCGGGATATGCAATTAACATTGCCTTGGATCCACTTCTCATCTTTGTATGCGGTTTTGGCATCAGAGGTGCAGCTATTGCACACGTTCTTTCTCAGTACTTGATGGCACTGGTCCTCTTCTTAATCTTAATGAGAAAAGTTAATCTCATGCCACCAAGTGTTAAAGACTTGCAGTTTGGCAAGTTTCTTAAGAATGGTTCTCTATTGTTGGCTAGGGTTGTTGCTGTGACATTCTGTGTCACCTTAGCTGCATCTTTGGCAGCACGGCAGGGTCCAACTCCCATGGCTGCATTCCAGACATGCTTACAAGTCTGGTTGACATCATCCCTTCTTGCTGATGGGCTAGCTGTTGCAGGACAGGCTATTCTAGCATGTGCATTTACTGAGAAAGACTACAAAAAGGCCACAGCTACCGCAACTCGGGTATTACAGATGGGATTTATCCTTGGTGTGGGACTCGCCCTTGTTGTTGGAGTTGGTTTGTACTTTGGAGCTGGAATCTTCTCAAGAGATGTCAATGTTTTGCACCTTATAAAAATTGGCCTCCCTTTTGTTGCAGCAACACAACCAATCAATTCCCTCTCTTTTGTGTTTGATGGTGTCAACTTTGGAGCATCTGACTTTGCATACTCTGCATATTCCTTGGTTCTGGTGGCTATAGCAAGCATTGTTTCATTGTTCCTTCTCTCTAAATCCGATGGTTTTGTTGGCATTTGGATTGCTTTGACTATCTATATGGGGTTGCGTGCATTCGCTGGCGTATGGAGGATGGGGACTGGAACCGGACCCTGGCGCTTTCTCAAGGGTCGTTCATCACCACAGCTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 map01524
Pfam Domains
Protein Families
DNA_gyraseB DNA_topoisoIV HATPase_c TOPRIM_C Toprim

Protein Analysis

594

Amino Acids

63.58

Weight (kDa)

9.1

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 238 - 330 1.2e-12 MatE
MatE PF01554 392 - 542 2.5e-10 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1487
AarI CACCTGC 1 cut(s) 883
Acc36I ACCTGC 1 cut(s) 883
AccB7I CCANNNNNTGG 1 cut(s) 355
AciI CCGC 5 cut(s) 215, 714, 956, 1005, 1368
AclWI GGATC 5 cut(s) 149, 774, 787, 977, 990
AcoI YGGCCR 1 cut(s) 894
AcsI RAATTY 1 cut(s) 547
AcuI CTGAAG 1 cut(s) 49
AdeI CACNNNGTG 1 cut(s) 1112
AfaI GTAC 3 cut(s) 848, 1052, 1439
AfiI CCNNNNNNNGG 4 cut(s) 355, 1374, 1405, 1743
AflII CTTAAG 1 cut(s) 1142
AflIII ACRYGT 1 cut(s) 1036
AgeI ACCGGT 1 cut(s) 644
AgsI TTSAA 4 cut(s) 88, 244, 255, 641
AjnI CCWGG 2 cut(s) 384, 1742
AloI GAACNNNNNNTCC 2 cut(s) 771, 803
Alw21I GWGCWC 1 cut(s) 874
Alw26I GTCTC 1 cut(s) 410
Alw44I GTGCAC 1 cut(s) 870
AlwI GGATC 5 cut(s) 149, 774, 787, 977, 990
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 1374
AoxI GGCC 5 cut(s) 346, 748, 894, 1356, 1495
ApaLI GTGCAC 1 cut(s) 870
ApeKI GCWGC 9 cut(s) 10, 134, 149, 218, 1025, 1202, 1214, 1241, 1511
ApoI RAATTY 1 cut(s) 547
ArsI GACNNNNNNTTYG 2 cut(s) 1112, 1144
AsiGI ACCGGT 1 cut(s) 644
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 1749
AspS9I GGNCC 5 cut(s) 200, 647, 1066, 1226, 1739
AsuHPI GGTGA 5 cut(s) 277, 461, 515, 1186, 1761
AsuNHI GCTAGC 1 cut(s) 1297
AvaI CYCGRG 1 cut(s) 1374
AvaII GGWCC 5 cut(s) 200, 647, 1066, 1226, 1739
BaeGI GKGCMC 1 cut(s) 874
BalI TGGCCA 1 cut(s) 896
BamHI GGATCC 2 cut(s) 779, 982
BbsI GAAGAC 1 cut(s) 495
Bbv12I GWGCWC 1 cut(s) 874
BbvI GCAGC 9 cut(s) 22, 121, 136, 230, 1037, 1189, 1226, 1228, 1523
BccI CCATC 8 cut(s) 658, 796, 1051, 1286, 1381, 1544, 1646, 1717
BceAI ACGGC 1 cut(s) 1235
BciT130I CCWGG 2 cut(s) 386, 1744
BciVI GTATCC 1 cut(s) 919
BcoDI GTCTC 1 cut(s) 410
BfaI CTAG 6 cut(s) 212, 434, 524, 1167, 1298, 1322
BfmI CTRYAG 4 cut(s) 116, 144, 315, 1611
BfoI RGCGCY 1 cut(s) 1750
BfrI CTTAAG 1 cut(s) 1142
BfuAI ACCTGC 1 cut(s) 883
BfuI GTATCC 1 cut(s) 919
BlpI GCTNAGC 1 cut(s) 567
BmcAI AGTACT 2 cut(s) 848, 1052
Bme1390I CCNGG 2 cut(s) 386, 1744
Bme18I GGWCC 5 cut(s) 200, 647, 1066, 1226, 1739
BmeT110I CYCGRG 1 cut(s) 1374
BmgT120I GGNCC 5 cut(s) 200, 647, 1066, 1226, 1739
BmiI GGNNCC 6 cut(s) 625, 781, 984, 1227, 1735, 1741
BmrFI CCNGG 2 cut(s) 386, 1744
BmsI GCATC 7 cut(s) 36, 61, 481, 725, 1023, 1214, 1577
BmtI GCTAGC 1 cut(s) 1301
BpiI GAAGAC 1 cut(s) 495
BpmI CTGGAG 1 cut(s) 593
Bpu10I CCTNAGC 1 cut(s) 1198
Bpu1102I GCTNAGC 1 cut(s) 567
BpuEI CTTGAG 3 cut(s) 871, 1444, 1739
BsaJI CCNNGG 7 cut(s) 303, 913, 978, 1236, 1399, 1596, 1742
BsaWI WCCGGW 2 cut(s) 644, 1736
BsaXI ACNNNNNCTCC 4 cut(s) 735, 765, 919, 949
Bsc4I CCNNNNNNNGG 4 cut(s) 355, 1374, 1405, 1743
Bse118I RCCGGY 1 cut(s) 644
Bse1I ACTGG 3 cut(s) 203, 1068, 1735
Bse3DI GCAATG 2 cut(s) 846, 972
BseBI CCWGG 2 cut(s) 386, 1744
BseDI CCNNGG 7 cut(s) 303, 913, 978, 1236, 1399, 1596, 1742
BseGI GGATG 4 cut(s) 76, 100, 1277, 1728
BseLI CCNNNNNNNGG 4 cut(s) 355, 1374, 1405, 1743
BseMI GCAATG 2 cut(s) 846, 972
BseMII CTCAG 5 cut(s) 405, 471, 525, 1061, 1329
BseNI ACTGG 3 cut(s) 203, 1068, 1735
BseSI GKGCMC 1 cut(s) 874
BseXI GCAGC 9 cut(s) 22, 121, 136, 230, 1037, 1189, 1226, 1228, 1523
BsgI GTGCAG 2 cut(s) 414, 1044
Bsh1285I CGRYCG 1 cut(s) 867
BshFI GGCC 5 cut(s) 348, 750, 896, 1358, 1497
BshTI ACCGGT 1 cut(s) 644
BsiEI CGRYCG 1 cut(s) 867
BsiHKAI GWGCWC 1 cut(s) 874
BsiHKCI CYCGRG 1 cut(s) 1374
BsiSI CCGG 2 cut(s) 645, 1737
BslFI GGGAC 3 cut(s) 113, 1422, 1741
BslI CCNNNNNNNGG 4 cut(s) 355, 1374, 1405, 1743
BsmAI GTCTC 1 cut(s) 410
BsmBI CGTCTC 1 cut(s) 410
BsmFI GGGAC 3 cut(s) 113, 1422, 1741
BsmI GAATGC 2 cut(s) 1244, 1703
BsnI GGCC 5 cut(s) 348, 750, 896, 1358, 1497
BsoBI CYCGRG 1 cut(s) 1374
Bsp1286I GDGCHC 1 cut(s) 874
Bsp143I GATC 4 cut(s) 154, 779, 864, 982
Bsp1720I GCTNAGC 1 cut(s) 567
Bsp19I CCATGG 1 cut(s) 1236
BspACI CCGC 5 cut(s) 215, 714, 956, 1005, 1368
BspANI GGCC 5 cut(s) 348, 750, 896, 1358, 1497
BspCNI CTCAG 5 cut(s) 406, 470, 524, 1060, 1330
BspLI GGNNCC 6 cut(s) 625, 781, 984, 1227, 1735, 1741
BspMAI CTGCAG 1 cut(s) 120
BspMI ACCTGC 1 cut(s) 883
BspOI GCTAGC 1 cut(s) 1301
BspPI GGATC 5 cut(s) 149, 774, 787, 977, 990
BspTI CTTAAG 1 cut(s) 1142
BsrDI GCAATG 2 cut(s) 846, 972
BsrFI RCCGGY 1 cut(s) 644
BsrI ACTGG 3 cut(s) 203, 1068, 1735
BssAI RCCGGY 1 cut(s) 644
BssECI CCNNGG 7 cut(s) 303, 913, 978, 1236, 1399, 1596, 1742
BssMI GATC 4 cut(s) 154, 779, 864, 982
BssT1I CCWWGG 4 cut(s) 978, 1236, 1399, 1596
Bst2UI CCWGG 2 cut(s) 386, 1744
Bst4CI ACNGT 2 cut(s) 316, 605
Bst6I CTCTTC 1 cut(s) 1076
BstAFI CTTAAG 1 cut(s) 1142
BstAPI GCANNNNNTGC 4 cut(s) 19, 131, 1031, 1586
BstC8I GCNNGC 7 cut(s) 429, 470, 759, 1299, 1618, 1701, 1711
BstDEI CTNAG 7 cut(s) 414, 457, 511, 567, 1047, 1198, 1338
BstDSI CCRYGG 1 cut(s) 1236
BstF5I GGATG 4 cut(s) 76, 100, 1277, 1728
BstH2I RGCGCY 1 cut(s) 1750
BstHHI GCGC 1 cut(s) 1749
BstKTI GATC 4 cut(s) 157, 782, 867, 985
BstMAI GTCTC 1 cut(s) 410
BstMBI GATC 4 cut(s) 154, 779, 864, 982
BstMCI CGRYCG 1 cut(s) 867
BstNI CCWGG 2 cut(s) 386, 1744
BstNSI RCATGY 3 cut(s) 376, 1257, 1329
BstSCI CCNGG 2 cut(s) 384, 1742
BstSFI CTRYAG 4 cut(s) 116, 144, 315, 1611
BstSLI GKGCMC 1 cut(s) 874
BstV1I GCAGC 9 cut(s) 22, 121, 136, 230, 1037, 1189, 1226, 1228, 1523
BstV2I GAAGAC 1 cut(s) 495
BstX2I RGATCY 2 cut(s) 779, 982
BstYI RGATCY 2 cut(s) 779, 982
BsuI GTATCC 1 cut(s) 919
BsuRI GGCC 5 cut(s) 348, 750, 896, 1358, 1497
BtgI CCRYGG 1 cut(s) 1236
BtsCI GGATG 4 cut(s) 76, 100, 1277, 1728
BtsI GCAGTG 1 cut(s) 541
BtsIMutI CAGTG 4 cut(s) 210, 541, 610, 1061
BveI ACCTGC 1 cut(s) 883
Cac8I GCNNGC 7 cut(s) 429, 470, 759, 1299, 1618, 1701, 1711
CaiI CAGNNNCTG 1 cut(s) 131
CfoI GCGC 1 cut(s) 1749
Cfr10I RCCGGY 1 cut(s) 644
Cfr13I GGNCC 5 cut(s) 200, 647, 1066, 1226, 1739
Csp6I GTAC 3 cut(s) 847, 1051, 1438
CspAI ACCGGT 1 cut(s) 644
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviAII CATG 9 cut(s) 23, 373, 772, 815, 898, 1102, 1237, 1254, 1326
CviQI GTAC 3 cut(s) 847, 1051, 1438
DdeI CTNAG 7 cut(s) 414, 457, 511, 567, 1047, 1198, 1338
DpnI GATC 4 cut(s) 156, 781, 866, 984
DpnII GATC 4 cut(s) 154, 779, 864, 982
DraIII CACNNNGTG 1 cut(s) 1112
EaeI YGGCCR 1 cut(s) 894
Eam1104I CTCTTC 1 cut(s) 1076
EarI CTCTTC 1 cut(s) 1076
Eco130I CCWWGG 4 cut(s) 978, 1236, 1399, 1596
Eco47I GGWCC 5 cut(s) 200, 647, 1066, 1226, 1739
Eco57I CTGAAG 1 cut(s) 49
Eco88I CYCGRG 1 cut(s) 1374
EcoRI GAATTC 1 cut(s) 547
EcoRII CCWGG 2 cut(s) 384, 1742
EcoT14I CCWWGG 4 cut(s) 978, 1236, 1399, 1596
EcoT22I ATGCAT 1 cut(s) 24
ErhI CCWWGG 4 cut(s) 978, 1236, 1399, 1596
Esp3I CGTCTC 1 cut(s) 410
FaeI CATG 9 cut(s) 26, 376, 775, 818, 901, 1105, 1240, 1257, 1329
FalI AAGNNNNNCTT 2 cut(s) 777, 809
FaqI GGGAC 3 cut(s) 113, 1422, 1741
FatI CATG 9 cut(s) 22, 372, 771, 814, 897, 1101, 1236, 1253, 1325
FauI CCCGC 1 cut(s) 949
FokI GGATG 4 cut(s) 83, 107, 1264, 1735
FspBI CTAG 6 cut(s) 212, 434, 524, 1167, 1298, 1322
GlaI GCGC 1 cut(s) 1748
GsuI CTGGAG 1 cut(s) 593
HaeII RGCGCY 1 cut(s) 1750
HaeIII GGCC 5 cut(s) 348, 750, 896, 1358, 1497
HapII CCGG 2 cut(s) 645, 1737
HhaI GCGC 1 cut(s) 1749
Hin1II CATG 9 cut(s) 26, 376, 775, 818, 901, 1105, 1240, 1257, 1329
Hin6I GCGC 1 cut(s) 1747
HinP1I GCGC 1 cut(s) 1747
HincII GTYRAC 2 cut(s) 1272, 1558
HindII GTYRAC 2 cut(s) 1272, 1558
HindIII AAGCTT 2 cut(s) 249, 698
HinfI GANTC 5 cut(s) 452, 818, 906, 1410, 1452
HpaII CCGG 2 cut(s) 645, 1737
HphI GGTGA 5 cut(s) 277, 461, 515, 1186, 1761
Hpy166II GTNNAC 4 cut(s) 608, 872, 1272, 1558
Hpy188I TCNGA 8 cut(s) 370, 460, 514, 559, 914, 1019, 1573, 1651
Hpy188III TCNNGA 2 cut(s) 1249, 1461
Hpy8I GTNNAC 4 cut(s) 608, 872, 1272, 1558
HpyAV CCTTC 4 cut(s) 355, 524, 1292, 1646
HpyCH4III ACNGT 2 cut(s) 316, 605
HpyCH4IV ACGT 1 cut(s) 1038
HpyF3I CTNAG 7 cut(s) 414, 457, 511, 567, 1047, 1198, 1338
HpySE526I ACGT 1 cut(s) 1038
Hsp92II CATG 9 cut(s) 26, 376, 775, 818, 901, 1105, 1240, 1257, 1329
HspAI GCGC 1 cut(s) 1747
Kzo9I GATC 4 cut(s) 154, 779, 864, 982
LmnI GCTCC 2 cut(s) 1445, 1565
Lsp1109I GCAGC 9 cut(s) 22, 121, 136, 230, 1037, 1189, 1226, 1228, 1523
LweI GCATC 7 cut(s) 36, 61, 481, 725, 1023, 1214, 1577
MaeI CTAG 6 cut(s) 212, 434, 524, 1167, 1298, 1322
MaeII ACGT 1 cut(s) 1038
MaeIII GTNAC 3 cut(s) 658, 1180, 1192
MalI GATC 4 cut(s) 156, 781, 866, 984
MboI GATC 4 cut(s) 154, 779, 864, 982
MflI RGATCY 2 cut(s) 779, 982
MhlI GDGCHC 1 cut(s) 874
MlsI TGGCCA 1 cut(s) 896
MluCI AATT 5 cut(s) 353, 547, 966, 1491, 1528
MluNI TGGCCA 1 cut(s) 896
MlyI GAGTC 2 cut(s) 812, 1404
MmeI TCCRAC 2 cut(s) 1253, 1405
Mox20I TGGCCA 1 cut(s) 896
Mph1103I ATGCAT 1 cut(s) 24
MroXI GAANNNNTTC 1 cut(s) 83
MscI TGGCCA 1 cut(s) 896
MseI TTAA 8 cut(s) 170, 702, 969, 1076, 1082, 1095, 1116, 1143
Msp20I TGGCCA 1 cut(s) 896
MspCI CTTAAG 1 cut(s) 1142
MspI CCGG 2 cut(s) 645, 1737
MspR9I CCNGG 2 cut(s) 386, 1744
Mva1269I GAATGC 2 cut(s) 1244, 1703
MvaI CCWGG 2 cut(s) 386, 1744
NcoI CCATGG 1 cut(s) 1236
NdeII GATC 4 cut(s) 154, 779, 864, 982
NheI GCTAGC 1 cut(s) 1297
NlaIII CATG 9 cut(s) 26, 376, 775, 818, 901, 1105, 1240, 1257, 1329
NlaIV GGNNCC 6 cut(s) 625, 781, 984, 1227, 1735, 1741
NmeAIII GCCGAG 1 cut(s) 328
NmuCI GTSAC 2 cut(s) 1180, 1192
NsiI ATGCAT 1 cut(s) 24
NspI RCATGY 3 cut(s) 376, 1257, 1329
PaqCI CACCTGC 1 cut(s) 883
PctI GAATGC 2 cut(s) 1244, 1703
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 3 cut(s) 452, 906, 1452
PflMI CCANNNNNTGG 1 cut(s) 355
PinAI ACCGGT 1 cut(s) 644
Ple19I CGATCG 1 cut(s) 867
PleI GAGTC 2 cut(s) 812, 1404
PpsI GAGTC 2 cut(s) 812, 1404
PsiI TTATAA 1 cut(s) 1487
Psp6I CCWGG 2 cut(s) 384, 1742
PspGI CCWGG 2 cut(s) 384, 1742
PspN4I GGNNCC 6 cut(s) 625, 781, 984, 1227, 1735, 1741
PspPI GGNCC 5 cut(s) 200, 647, 1066, 1226, 1739
PstI CTGCAG 1 cut(s) 120
PstNI CAGNNNCTG 1 cut(s) 131
PsuI RGATCY 2 cut(s) 779, 982
PvuI CGATCG 1 cut(s) 867
RsaI GTAC 3 cut(s) 848, 1052, 1439
RsaNI GTAC 3 cut(s) 847, 1051, 1438
SaqAI TTAA 8 cut(s) 170, 702, 969, 1076, 1082, 1095, 1116, 1143
Sau3AI GATC 4 cut(s) 154, 779, 864, 982
Sau96I GGNCC 5 cut(s) 200, 647, 1066, 1226, 1739
ScaI AGTACT 2 cut(s) 848, 1052
SchI GAGTC 2 cut(s) 812, 1404
ScrFI CCNGG 2 cut(s) 386, 1744
SduI GDGCHC 1 cut(s) 874
SfaNI GCATC 7 cut(s) 36, 61, 481, 725, 1023, 1214, 1577
SfcI CTRYAG 4 cut(s) 116, 144, 315, 1611
SinI GGWCC 5 cut(s) 200, 647, 1066, 1226, 1739
SmlI CTYRAG 4 cut(s) 850, 1142, 1459, 1754
SmoI CTYRAG 4 cut(s) 850, 1142, 1459, 1754
Sse9I AATT 5 cut(s) 353, 547, 966, 1491, 1528
SsiI CCGC 5 cut(s) 215, 714, 956, 1005, 1368
SspI AATATT 2 cut(s) 637, 706
SspMI CTAG 6 cut(s) 212, 434, 524, 1167, 1298, 1322
StyD4I CCNGG 2 cut(s) 384, 1742
StyI CCWWGG 4 cut(s) 978, 1236, 1399, 1596
TaaI ACNGT 2 cut(s) 316, 605
TaiI ACGT 1 cut(s) 1041
TaqI TCGA 2 cut(s) 174, 863
TasI AATT 5 cut(s) 353, 547, 966, 1491, 1528
TatI WGTACW 3 cut(s) 846, 1050, 1437
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 3 cut(s) 452, 906, 1452
Tru1I TTAA 8 cut(s) 170, 702, 969, 1076, 1082, 1095, 1116, 1143
Tru9I TTAA 8 cut(s) 170, 702, 969, 1076, 1082, 1095, 1116, 1143
TscAI CASTG 4 cut(s) 210, 541, 610, 1068
TseFI GTSAC 2 cut(s) 1180, 1192
TseI GCWGC 9 cut(s) 10, 134, 149, 218, 1025, 1202, 1214, 1241, 1511
Tsp45I GTSAC 2 cut(s) 1180, 1192
TspRI CASTG 4 cut(s) 210, 541, 610, 1068
Van91I CCANNNNNTGG 1 cut(s) 355
Vha464I CTTAAG 1 cut(s) 1142
VneI GTGCAC 1 cut(s) 870
VpaK11BI GGWCC 5 cut(s) 200, 647, 1066, 1226, 1739
XapI RAATTY 1 cut(s) 547
XceI RCATGY 3 cut(s) 376, 1257, 1329
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 6 cut(s) 212, 434, 524, 1167, 1298, 1322
ZrmI AGTACT 2 cut(s) 848, 1052
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.