Rh1CG417400

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0001532
Physical Location & Seq
Reverse (-)
67811499 .. 67813352
1854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG417400.1

Sequence Viewer

Length: 1176 bp
ATGGCGGTGGAGGTCCCGGAGGACGCCCCCGCCCCGGGAGTCGACGGCGACATCGCCGCAGACCTCGATATGCAAGTCTCAGACGACGACGACGACACCTCAGACGGCGAACGCGACGACGAGAACGGCACCTCAGGCGATGAGAACGACGACATCGAGATCACAGAAGCTGACGAGAACGACGACGACGCCGAGAATCCAGACGACGACGACGACGGCGACGAGCTCATCCACGAAGTCGACAACGACGAGTCGTCGGAGGAAGAGGTGGAGTGCGATGAGACGTGGCTAGAGGAAGCTGAATTCGCCGGAGGGCGCAAGTCCCAAGAGTGTACTTTGATTTTGACACAAGGAATGTCAACTTCAAGTCTCGCACTAAACGACGTTGTTTCCATGAAGGGTTGGGAAAGGTATGGTGTTTACCCACTGGTAGGTGAATTGCTGAACGTGAGAGAAGCTGGCTTCTTTGAGGTCATGTATAATCCGATTTTCAAGACGATTATGAAGACTCTCGGACTTGAGCTAGATGAAGAATTTACCAGTACCGAATCTTTGAGATATGGCCGGGTCGTTATTATGACTGCTGAACAGGATCATTATGGTAGTTACATCAAAGGGTTGTTGATCAATTTTCTTCATATGTTCTGGCCATCATTGCTAAAGCTTCCGTCATTTTTGGCCGAGCTTATAGCTCCTCTTACCATCAAGGCAACAAAAGAGAGTGAGGAAGGGAAAGGAAAACGTGTATTCTATTCCCTGCAAGAGTATGAAGCTTGGAGGGAAAGTTTGAGGGGCAATGCGAGTGGCTGGGCCATTGAGAAGGATGTAGCATGCGTTTATGATGAAGGGAAGGATTATTTTGAAAATCTTTTAATGCAGAAGAAAGATTTTGTTTGGGTAGATGAACGTGATGATGAGGCAATTGAGCTTGCTTTCAGCAAGAAGTTTGAGGTGGGAAGTTTGCAGTCTGGTATGCATCTTGATCATGAGGAGAAACTTGTAACTTATAGTGACTTTGTTGACAAGGAGCTTATACCCTTTTCCATGCTTGCTGTTCTGCAACGCTCAATTCCGTCAATGGTTGATGGCTTGAAGCCTCATCAAAGAAAGGTTCTATTCAGCTCTTTTAAGAGGGACGACTTTATCAAGGAAGAATTTTGGAAAAGCCTCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 map01524
Pfam Domains
Protein Families
DNA_gyraseB DNA_topoisoIV HATPase_c TOPRIM_C Toprim

Protein Analysis

391

Amino Acids

44.37

Weight (kDa)

4.16

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOPRIM_C PF16898 235 - 350 8.1e-19 C-terminal associated domain of TOPRIM
DNA_topoisoIV PF00521 355 - 378 6.2e-07 DNA gyrase/topoisomerase IV, subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 128
AccI GTMKAC 2 cut(s) 42, 240
AccII CGCG 1 cut(s) 114
AciI CCGC 3 cut(s) 5, 30, 57
AclWI GGATC 1 cut(s) 600
AcoI YGGCCR 3 cut(s) 562, 647, 678
AcsI RAATTY 3 cut(s) 302, 533, 1154
AcyI GRCGYC 2 cut(s) 24, 189
AfaI GTAC 2 cut(s) 334, 544
AfiI CCNNNNNNNGG 3 cut(s) 34, 35, 431
AflIII ACRYGT 1 cut(s) 742
AgsI TTSAA 4 cut(s) 366, 493, 863, 1093
AhdI GACNNNNNGTC 1 cut(s) 253
AjiI CACGTC 1 cut(s) 285
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 3 cut(s) 82, 275, 374
AlwI GGATC 1 cut(s) 600
AlwNI CAGNNNCTG 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 34
AoxI GGCC 4 cut(s) 562, 647, 678, 810
ApoI RAATTY 3 cut(s) 302, 533, 1154
AspLEI GCGC 1 cut(s) 318
AspS9I GGNCC 2 cut(s) 13, 810
AsuC2I CCSGG 4 cut(s) 17, 35, 36, 566
AsuHPI GGTGA 1 cut(s) 446
AvaI CYCGRG 1 cut(s) 34
AvaII GGWCC 1 cut(s) 13
AxyI CCTNAGG 1 cut(s) 133
BalI TGGCCA 1 cut(s) 649
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 228
BbsI GAAGAC 1 cut(s) 512
Bbv12I GWGCWC 1 cut(s) 228
BccI CCATC 3 cut(s) 658, 710, 1079
BceAI ACGGC 4 cut(s) 61, 121, 142, 232
BclI TGATCA 2 cut(s) 624, 982
BcnI CCSGG 4 cut(s) 17, 35, 36, 566
BcoDI GTCTC 3 cut(s) 82, 275, 374
BfaI CTAG 2 cut(s) 290, 524
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
Bme1390I CCNGG 4 cut(s) 17, 35, 36, 566
Bme18I GGWCC 1 cut(s) 13
BmeRI GACNNNNNGTC 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 34
BmgBI CACGTC 1 cut(s) 285
BmgT120I GGNCC 2 cut(s) 13, 810
BmiI GGNNCC 2 cut(s) 15, 130
BmrFI CCNGG 4 cut(s) 17, 35, 36, 566
BmsI GCATC 1 cut(s) 985
BpiI GAAGAC 1 cut(s) 512
BpuEI CTTGAG 1 cut(s) 539
BpuMI CCSGG 4 cut(s) 17, 35, 36, 566
BsaHI GRCGYC 2 cut(s) 24, 189
BsaJI CCNNGG 2 cut(s) 33, 34
BsaXI ACNNNNNCTCC 2 cut(s) 769, 799
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 35, 431
Bse1I ACTGG 2 cut(s) 432, 540
Bse21I CCTNAGG 1 cut(s) 133
Bse3DI GCAATG 2 cut(s) 653, 802
BseDI CCNNGG 2 cut(s) 33, 34
BseGI GGATG 2 cut(s) 228, 829
BseLI CCNNNNNNNGG 3 cut(s) 34, 35, 431
BseMI GCAATG 2 cut(s) 653, 802
BseMII CTCAG 3 cut(s) 93, 114, 147
BseNI ACTGG 2 cut(s) 432, 540
BseRI GAGGAG 2 cut(s) 684, 1004
BseYI CCCAGC 1 cut(s) 807
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 4 cut(s) 564, 649, 680, 812
BshNI GGYRCC 1 cut(s) 128
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 34
BsiSI CCGG 4 cut(s) 17, 35, 309, 565
BslFI GGGAC 2 cut(s) 307, 1148
BslI CCNNNNNNNGG 3 cut(s) 34, 35, 431
BsmAI GTCTC 3 cut(s) 82, 275, 374
BsmBI CGTCTC 1 cut(s) 275
BsmFI GGGAC 2 cut(s) 307, 1148
BsnI GGCC 4 cut(s) 564, 649, 680, 812
BsoBI CYCGRG 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 228
Bsp143I GATC 4 cut(s) 159, 592, 624, 982
BspACI CCGC 3 cut(s) 5, 30, 57
BspANI GGCC 4 cut(s) 564, 649, 680, 812
BspCNI CTCAG 3 cut(s) 92, 113, 146
BspFNI CGCG 1 cut(s) 114
BspHI TCATGA 1 cut(s) 985
BspLI GGNNCC 2 cut(s) 15, 130
BspPI GGATC 1 cut(s) 600
BspT107I GGYRCC 1 cut(s) 128
BsrDI GCAATG 2 cut(s) 653, 802
BsrI ACTGG 2 cut(s) 432, 540
BssECI CCNNGG 2 cut(s) 33, 34
BssMI GATC 4 cut(s) 159, 592, 624, 982
BssNI GRCGYC 2 cut(s) 24, 189
Bst6I CTCTTC 1 cut(s) 258
BstACI GRCGYC 2 cut(s) 24, 189
BstC8I GCNNGC 4 cut(s) 460, 832, 930, 1050
BstDEI CTNAG 3 cut(s) 79, 100, 133
BstF5I GGATG 2 cut(s) 228, 829
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 4 cut(s) 162, 595, 627, 985
BstMAI GTCTC 3 cut(s) 82, 275, 374
BstMBI GATC 4 cut(s) 159, 592, 624, 982
BstMWI GCNNNNNNNGC 3 cut(s) 135, 305, 655
BstNSI RCATGY 1 cut(s) 834
BstSCI CCNGG 4 cut(s) 15, 33, 34, 564
BstUI CGCG 1 cut(s) 114
BstV2I GAAGAC 1 cut(s) 512
Bsu36I CCTNAGG 1 cut(s) 133
BsuRI GGCC 4 cut(s) 564, 649, 680, 812
BtgZI GCGATG 3 cut(s) 37, 153, 291
BtrI CACGTC 1 cut(s) 285
BtsCI GGATG 2 cut(s) 228, 829
BtsIMutI CAGTG 1 cut(s) 425
Cac8I GCNNGC 4 cut(s) 460, 832, 930, 1050
CaiI CAGNNNCTG 1 cut(s) 170
CciI TCATGA 1 cut(s) 985
CfoI GCGC 1 cut(s) 318
Cfr13I GGNCC 2 cut(s) 13, 810
Cfr9I CCCGGG 1 cut(s) 34
CseI GACGC 2 cut(s) 32, 197
Csp6I GTAC 2 cut(s) 333, 543
CspCI CAANNNNNGTGG 2 cut(s) 784, 819
CviAII CATG 5 cut(s) 394, 475, 831, 986, 1045
CviQI GTAC 2 cut(s) 333, 543
DdeI CTNAG 3 cut(s) 79, 100, 133
DpnI GATC 4 cut(s) 161, 594, 626, 984
DpnII GATC 4 cut(s) 159, 592, 624, 982
DriI GACNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 3 cut(s) 562, 647, 678
Eam1104I CTCTTC 1 cut(s) 258
Eam1105I GACNNNNNGTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 258
Ecl136II GAGCTC 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 228
Eco47I GGWCC 1 cut(s) 13
Eco53kI GAGCTC 1 cut(s) 226
Eco81I CCTNAGG 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 302
EcoT22I ATGCAT 1 cut(s) 978
EcoT38I GRGCYC 1 cut(s) 228
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 5 cut(s) 397, 478, 834, 989, 1048
FaqI GGGAC 2 cut(s) 307, 1148
FatI CATG 5 cut(s) 393, 474, 830, 985, 1044
FauI CCCGC 1 cut(s) 37
FauNDI CATATG 1 cut(s) 639
FbaI TGATCA 2 cut(s) 624, 982
FblI GTMKAC 2 cut(s) 42, 240
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 2 cut(s) 215, 836
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 2 cut(s) 290, 524
GlaI GCGC 1 cut(s) 317
GluI GCNGC 1 cut(s) 57
GsaI CCCAGC 1 cut(s) 811
HaeIII GGCC 4 cut(s) 564, 649, 680, 812
HapII CCGG 4 cut(s) 17, 35, 309, 565
HgaI GACGC 2 cut(s) 32, 197
HhaI GCGC 1 cut(s) 318
Hin1I GRCGYC 2 cut(s) 24, 189
Hin1II CATG 5 cut(s) 397, 478, 834, 989, 1048
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HincII GTYRAC 4 cut(s) 43, 241, 360, 1021
HindII GTYRAC 4 cut(s) 43, 241, 360, 1021
HindIII AAGCTT 2 cut(s) 662, 771
HinfI GANTC 5 cut(s) 39, 196, 251, 508, 548
HpaII CCGG 4 cut(s) 17, 35, 309, 565
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 6 cut(s) 43, 241, 333, 360, 421, 1021
Hpy188I TCNGA 5 cut(s) 82, 103, 259, 486, 515
Hpy188III TCNNGA 5 cut(s) 157, 200, 493, 980, 986
Hpy8I GTNNAC 6 cut(s) 43, 241, 333, 360, 421, 1021
HpyAV CCTTC 5 cut(s) 391, 722, 814, 839, 844
HpyCH4IV ACGT 5 cut(s) 284, 384, 447, 742, 907
HpyCH4V TGCA 6 cut(s) 73, 760, 877, 964, 976, 1060
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 305, 655
HpyF3I CTNAG 3 cut(s) 79, 100, 133
HpySE526I ACGT 5 cut(s) 284, 384, 447, 742, 907
Hsp92I GRCGYC 2 cut(s) 24, 189
Hsp92II CATG 5 cut(s) 397, 478, 834, 989, 1048
HspAI GCGC 1 cut(s) 316
Ksp22I TGATCA 2 cut(s) 624, 982
Kzo9I GATC 4 cut(s) 159, 592, 624, 982
LmnI GCTCC 2 cut(s) 697, 1027
LweI GCATC 1 cut(s) 985
MaeI CTAG 2 cut(s) 290, 524
MaeII ACGT 5 cut(s) 284, 384, 447, 742, 907
MaeIII GTNAC 3 cut(s) 605, 1000, 1010
MalI GATC 4 cut(s) 161, 594, 626, 984
MboI GATC 4 cut(s) 159, 592, 624, 982
MboII GAAGA 6 cut(s) 275, 517, 542, 626, 892, 1163
MfeI CAATTG 1 cut(s) 921
MhlI GDGCHC 1 cut(s) 228
MlsI TGGCCA 1 cut(s) 649
MluCI AATT 7 cut(s) 302, 437, 533, 628, 921, 1068, 1154
MluNI TGGCCA 1 cut(s) 649
MlyI GAGTC 3 cut(s) 48, 260, 502
MmeI TCCRAC 1 cut(s) 237
Mox20I TGGCCA 1 cut(s) 649
Mph1103I ATGCAT 1 cut(s) 978
MscI TGGCCA 1 cut(s) 649
MseI TTAA 2 cut(s) 872, 1128
Msp20I TGGCCA 1 cut(s) 649
MspI CCGG 4 cut(s) 17, 35, 309, 565
MspR9I CCNGG 4 cut(s) 17, 35, 36, 566
MunI CAATTG 1 cut(s) 921
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 3 cut(s) 135, 305, 655
NciI CCSGG 4 cut(s) 17, 35, 36, 566
NdeI CATATG 1 cut(s) 639
NdeII GATC 4 cut(s) 159, 592, 624, 982
NlaIII CATG 5 cut(s) 397, 478, 834, 989, 1048
NlaIV GGNNCC 2 cut(s) 15, 130
NmeAIII GCCGAG 2 cut(s) 217, 706
NmuCI GTSAC 1 cut(s) 1010
NsiI ATGCAT 1 cut(s) 978
NspI RCATGY 1 cut(s) 834
PaeI GCATGC 1 cut(s) 834
PagI TCATGA 1 cut(s) 985
PfeI GAWTC 2 cut(s) 196, 548
PfoI TCCNGGA 1 cut(s) 15
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 3 cut(s) 47, 259, 502
PpsI GAGTC 3 cut(s) 47, 259, 502
PpuMI RGGWCCY 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 228
Psp5II RGGWCCY 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 807
PspN4I GGNNCC 2 cut(s) 15, 130
PspPI GGNCC 2 cut(s) 13, 810
PspPPI RGGWCCY 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 170
RsaI GTAC 2 cut(s) 334, 544
RsaNI GTAC 2 cut(s) 333, 543
SacI GAGCTC 1 cut(s) 228
SalI GTCGAC 2 cut(s) 41, 239
SaqAI TTAA 2 cut(s) 872, 1128
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 4 cut(s) 159, 592, 624, 982
Sau96I GGNCC 2 cut(s) 13, 810
SchI GAGTC 3 cut(s) 48, 260, 502
ScrFI CCNGG 4 cut(s) 17, 35, 36, 566
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 1 cut(s) 985
SinI GGWCC 1 cut(s) 13
SmaI CCCGGG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
SphI GCATGC 1 cut(s) 834
Sse9I AATT 7 cut(s) 302, 437, 533, 628, 921, 1068, 1154
SsiI CCGC 3 cut(s) 5, 30, 57
SspMI CTAG 2 cut(s) 290, 524
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 4 cut(s) 15, 33, 34, 564
TaiI ACGT 5 cut(s) 287, 387, 450, 745, 910
TaqI TCGA 4 cut(s) 42, 66, 156, 240
TasI AATT 7 cut(s) 302, 437, 533, 628, 921, 1068, 1154
TatI WGTACW 1 cut(s) 332
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 2 cut(s) 196, 548
Tru1I TTAA 2 cut(s) 872, 1128
Tru9I TTAA 2 cut(s) 872, 1128
TscAI CASTG 1 cut(s) 432
TseFI GTSAC 1 cut(s) 1010
Tsp45I GTSAC 1 cut(s) 1010
TspDTI ATGAA 7 cut(s) 410, 518, 543, 626, 783, 858, 918
TspGWI ACGGA 2 cut(s) 657, 1062
TspMI CCCGGG 1 cut(s) 34
TspRI CASTG 1 cut(s) 432
VpaK11BI GGWCC 1 cut(s) 13
XapI RAATTY 3 cut(s) 302, 533, 1154
XceI RCATGY 1 cut(s) 834
XmaI CCCGGG 1 cut(s) 34
XmiI GTMKAC 2 cut(s) 42, 240
XspI CTAG 2 cut(s) 290, 524
Zsp2I ATGCAT 1 cut(s) 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.